Rmu_sc0002434.1_g000007

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002434.1
OG0000593
Physical Location & Seq
Forward (+)
20119 .. 25639
5521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002434.1_g000007.1.cds

Sequence Viewer

Length: 1506 bp
atgggagactttgccgaaaccccgtcaccggccgccggaatcctgtcaactttcgccggattccggtcaactttcgccagattcctgtcacccactgcctcccaccggaatttgctgaaaatctcgtctgaaagtttttttagccccaatagacatctattgccccctaatagagggcctgggtcgagcaccggcggcgtccaggaggccatcgaagtcgtcgttgttggcaccggccaagctgctgcagcagaatccgggctaaagtcaacccgcaccggagaggctgttggactcggtcgagctcaccggcgagttgcacggaatctgggagttgcagcgaaaatccctagagcatttctccagtattttaatggaaaggtacctcccaagtgctttcttcaaaccctaggtcaaacttggcttgttgatgtggaaaaggttaatcacaagttctatttccaactggggtggaatcgctttgtgcaagacaatggcttgaagtatggtcaacttctagtcttccgttatgctggaaactcaaacttccatgtggacatatttggaataaatacctgcaaaaatctgtgtgtaattcctgagatggaagaagctaatgaatatgatgatgatgtatctcttgagatcttggacgactttccaccctgtcaaagaaaaacagaggagaaatctttgctttcatgtcctctgcctcgcaagaaaaacaaaacaagttctagtggtagagcagatatcacccctaactttagaaagagggttccacatcttgagaagtcacaaatgaaaaggaaaaaggcacattggttcaaatcagaaatgataggtgcggaatacaactataattctgatactgattctggtgatgaagatgatcatgatgaggaggatgacgacgaaaatgaagatgatgaagaagaagaagaagaaactcaagatgaagaggttgaggaggaagatgatggtgattctgttggaaccctcaacgattctctaccatgctcaagaacagtggggaaatatacattagcatcgaggctgcaccagagagggagaaagatttctagtgcaaaagcaaaaaccaacatcaaaactgttgatggcttgtctagagaggaaaagatcctaaaagttatagctctacaaagagctaatgctttcaaatctaattctgtcaacccttgtttcatggtttccttgtctgccacatatgcttgtggagcttttctgaatttgccacggagttttgctgcagtacattttactaatccgcagcctagccatgtcatcctttgggttgagaagagctcttggtttgtcaaggtcagttatattgggactgcaacgagattacaggccggctggtctgaatttgtgcatgtcaataatttgaagattggtgatgcttgtgtttttgtcttgacggatgccatcaattttcaatatgatgttgaaatattccgcgttacagatgctgtttgctcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

501

Amino Acids

55.82

Weight (kDa)

5.74

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 584
Acc65I GGTACC 1 cut(s) 382
AccB1I GGYRCC 2 cut(s) 230, 382
AccII CGCG 1 cut(s) 1482
AciI CCGC 6 cut(s) 33, 195, 274, 848, 1289, 1480
AclWI GGATC 1 cut(s) 1135
AcoI YGGCCR 2 cut(s) 30, 235
AcsI RAATTY 3 cut(s) 109, 1249, 1388
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 384, 1275
AfiI CCNNNNNNNGG 5 cut(s) 28, 35, 63, 105, 173
AgsI TTSAA 7 cut(s) 404, 502, 829, 1180, 1411, 1460, 1472
AjnI CCWGG 2 cut(s) 178, 201
AluBI AGCT 7 cut(s) 242, 305, 614, 1157, 1169, 1241, 1326
AluI AGCT 7 cut(s) 242, 305, 614, 1157, 1169, 1241, 1326
Alw21I GWGCWC 3 cut(s) 191, 307, 1328
AlwI GGATC 1 cut(s) 1135
AlwNI CAGNNNCTG 1 cut(s) 1493
AoxI GGCC 5 cut(s) 30, 176, 207, 235, 1374
ApeKI GCWGC 7 cut(s) 242, 245, 248, 338, 1057, 1268, 1291
ApoI RAATTY 3 cut(s) 109, 1249, 1388
ArsI GACNNNNNNTTYG 2 cut(s) 397, 429
Asp700I GAANNNNTTC 1 cut(s) 1078
Asp718I GGTACC 1 cut(s) 382
AspA2I CCTAGG 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 7 cut(s) 18, 81, 299, 748, 893, 995, 1430
AvrII CCTAGG 1 cut(s) 409
BanI GGYRCC 2 cut(s) 230, 382
BanII GRGCYC 2 cut(s) 307, 1328
BbsI GAAGAC 1 cut(s) 514
Bbv12I GWGCWC 3 cut(s) 191, 307, 1328
BbvI GCAGC 7 cut(s) 229, 232, 260, 350, 1044, 1255, 1303
BccI CCATC 5 cut(s) 218, 598, 974, 1112, 1457
BciT130I CCWGG 2 cut(s) 180, 203
BclI TGATCA 1 cut(s) 892
BcnI CCSGG 1 cut(s) 259
BfaI CTAG 8 cut(s) 351, 410, 518, 738, 1083, 1128, 1296, 1504
BfmI CTRYAG 2 cut(s) 246, 1269
BfuAI ACCTGC 1 cut(s) 584
BglII AGATCT 1 cut(s) 645
BisI GCNGC 9 cut(s) 33, 196, 243, 246, 249, 339, 1058, 1269, 1292
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 9 cut(s) 34, 197, 244, 247, 250, 340, 1059, 1270, 1293
Bme1390I CCNGG 3 cut(s) 180, 203, 259
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 4 cut(s) 232, 384, 780, 997
BmrFI CCNGG 3 cut(s) 180, 203, 259
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 4 cut(s) 1058, 1411, 1435, 1480
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 514
BplI GAGNNNNNCTC 4 cut(s) 345, 377, 1310, 1342
BpmI CTGGAG 1 cut(s) 347
BpuEI CTTGAG 4 cut(s) 662, 809, 936, 1006
BpuMI CCSGG 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 179, 409, 1256
BsaWI WCCGGW 3 cut(s) 63, 105, 278
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 35, 63, 105, 173
Bse118I RCCGGY 5 cut(s) 28, 191, 233, 309, 1376
Bse1I ACTGG 2 cut(s) 364, 471
BseBI CCWGG 2 cut(s) 180, 203
BseDI CCNNGG 3 cut(s) 179, 409, 1256
BseGI GGATG 3 cut(s) 913, 1305, 1450
BseLI CCNNNNNNNGG 5 cut(s) 28, 35, 63, 105, 173
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 364, 471
BseRI GAGGAG 3 cut(s) 698, 917, 983
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 7 cut(s) 229, 232, 260, 350, 1044, 1255, 1303
BsgI GTGCAG 1 cut(s) 1043
Bsh1236I CGCG 1 cut(s) 1482
Bsh1285I CGRYCG 2 cut(s) 33, 301
BshFI GGCC 5 cut(s) 32, 178, 209, 237, 1376
BshNI GGYRCC 2 cut(s) 230, 382
BsiEI CGRYCG 2 cut(s) 33, 301
BsiHKAI GWGCWC 3 cut(s) 191, 307, 1328
BslFI GGGAC 1 cut(s) 1369
BslI CCNNNNNNNGG 5 cut(s) 28, 35, 63, 105, 173
BsmFI GGGAC 1 cut(s) 1369
BsnI GGCC 5 cut(s) 32, 178, 209, 237, 1376
Bsp1286I GDGCHC 3 cut(s) 191, 307, 1328
Bsp143I GATC 3 cut(s) 645, 892, 1140
BspACI CCGC 6 cut(s) 33, 195, 274, 848, 1289, 1480
BspANI GGCC 5 cut(s) 32, 178, 209, 237, 1376
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 1 cut(s) 1482
BspHI TCATGA 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 232, 384, 780, 997
BspMAI CTGCAG 2 cut(s) 250, 1273
BspMI ACCTGC 1 cut(s) 584
BspPI GGATC 1 cut(s) 1135
BspQI GCTCTTC 1 cut(s) 1316
BspT107I GGYRCC 2 cut(s) 230, 382
BsrFI RCCGGY 5 cut(s) 28, 191, 233, 309, 1376
BsrI ACTGG 2 cut(s) 364, 471
BssAI RCCGGY 5 cut(s) 28, 191, 233, 309, 1376
BssECI CCNNGG 3 cut(s) 179, 409, 1256
BssMI GATC 3 cut(s) 645, 892, 1140
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 2 cut(s) 180, 203
Bst4CI ACNGT 2 cut(s) 1030, 1114
Bst6I CTCTTC 2 cut(s) 954, 1316
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 1378
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 1256
BstENI CCTNNNNNAGG 1 cut(s) 171
BstF5I GGATG 3 cut(s) 913, 1305, 1450
BstFNI CGCG 1 cut(s) 1482
BstKTI GATC 3 cut(s) 648, 895, 1143
BstMBI GATC 3 cut(s) 645, 892, 1140
BstMCI CGRYCG 2 cut(s) 33, 301
BstMWI GCNNNNNNNGC 4 cut(s) 195, 248, 1229, 1238
BstNI CCWGG 2 cut(s) 180, 203
BstNSI RCATGY 1 cut(s) 1400
BstSCI CCNGG 3 cut(s) 178, 201, 257
BstSFI CTRYAG 2 cut(s) 246, 1269
BstUI CGCG 1 cut(s) 1482
BstV1I GCAGC 7 cut(s) 229, 232, 260, 350, 1044, 1255, 1303
BstV2I GAAGAC 1 cut(s) 514
BstX2I RGATCY 2 cut(s) 645, 1140
BstYI RGATCY 2 cut(s) 645, 1140
BstZI CGGCCG 1 cut(s) 30
BsuRI GGCC 5 cut(s) 32, 178, 209, 237, 1376
BtgI CCRYGG 1 cut(s) 1256
BtsCI GGATG 3 cut(s) 913, 1305, 1450
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 1035
BveI ACCTGC 1 cut(s) 584
Cac8I GCNNGC 1 cut(s) 1378
CaiI CAGNNNCTG 1 cut(s) 1493
CciI TCATGA 1 cut(s) 895
Cfr10I RCCGGY 5 cut(s) 28, 191, 233, 309, 1376
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 187
Csp6I GTAC 2 cut(s) 383, 1274
CspCI CAANNNNNGTGG 4 cut(s) 452, 487, 1011, 1046
CviAII CATG 7 cut(s) 551, 702, 896, 1017, 1207, 1301, 1397
CviQI GTAC 2 cut(s) 383, 1274
DdeI CTNAG 1 cut(s) 600
DpnI GATC 3 cut(s) 647, 894, 1142
DpnII GATC 3 cut(s) 645, 892, 1140
EaeI YGGCCR 2 cut(s) 30, 235
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 954, 1316
EarI CTCTTC 2 cut(s) 954, 1316
Ecl136II GAGCTC 2 cut(s) 305, 1326
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 2 cut(s) 307, 1328
Eco32I GATATC 1 cut(s) 754
Eco52I CGGCCG 1 cut(s) 30
Eco53kI GAGCTC 2 cut(s) 305, 1326
EcoICRI GAGCTC 2 cut(s) 305, 1326
EcoNI CCTNNNNNAGG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 178, 201
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 1 cut(s) 409
EcoT38I GRGCYC 2 cut(s) 307, 1328
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 7 cut(s) 554, 705, 899, 1020, 1210, 1304, 1400
FaqI GGGAC 1 cut(s) 1369
FatI CATG 7 cut(s) 550, 701, 895, 1016, 1206, 1300, 1396
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 1 cut(s) 1228
FbaI TGATCA 1 cut(s) 892
Fnu4HI GCNGC 9 cut(s) 33, 196, 243, 246, 249, 339, 1058, 1269, 1292
FokI GGATG 3 cut(s) 920, 1292, 1457
FriOI GRGCYC 2 cut(s) 307, 1328
Fsp4HI GCNGC 9 cut(s) 33, 196, 243, 246, 249, 339, 1058, 1269, 1292
FspBI CTAG 8 cut(s) 351, 410, 518, 738, 1083, 1128, 1296, 1504
GluI GCNGC 9 cut(s) 33, 196, 243, 246, 249, 339, 1058, 1269, 1292
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 5 cut(s) 32, 178, 209, 237, 1376
HgaI GACGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 7 cut(s) 554, 705, 899, 1020, 1210, 1304, 1400
HincII GTYRAC 5 cut(s) 48, 69, 270, 512, 1195
HindII GTYRAC 5 cut(s) 48, 69, 270, 512, 1195
HphI GGTGA 7 cut(s) 18, 81, 299, 748, 893, 995, 1430
Hpy166II GTNNAC 6 cut(s) 48, 69, 270, 512, 556, 1195
Hpy188I TCNGA 5 cut(s) 130, 835, 868, 1248, 1387
Hpy188III TCNNGA 8 cut(s) 599, 641, 788, 896, 953, 1023, 1128, 1438
Hpy8I GTNNAC 6 cut(s) 48, 69, 270, 512, 556, 1195
Hpy99I CGWCG 2 cut(s) 224, 917
HpyCH4III ACNGT 2 cut(s) 1030, 1114
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 248, 1229, 1238
HpyF3I CTNAG 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 7 cut(s) 554, 705, 899, 1020, 1210, 1304, 1400
KpnI GGTACC 1 cut(s) 386
KroI GCCGGC 1 cut(s) 1376
KroNI GCCGGC 1 cut(s) 1378
Ksp22I TGATCA 1 cut(s) 892
Kzo9I GATC 3 cut(s) 645, 892, 1140
LguI GCTCTTC 1 cut(s) 1316
LmnI GCTCC 2 cut(s) 1238, 1505
Lsp1109I GCAGC 7 cut(s) 229, 232, 260, 350, 1044, 1255, 1303
LweI GCATC 4 cut(s) 1058, 1411, 1435, 1480
MaeI CTAG 8 cut(s) 351, 410, 518, 738, 1083, 1128, 1296, 1504
MaeIII GTNAC 4 cut(s) 24, 87, 795, 1483
MalI GATC 3 cut(s) 647, 894, 1142
MboI GATC 3 cut(s) 645, 892, 1140
MflI RGATCY 2 cut(s) 645, 1140
MhlI GDGCHC 3 cut(s) 191, 307, 1328
MluCI AATT 8 cut(s) 109, 594, 862, 1186, 1249, 1388, 1405, 1453
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 3 cut(s) 271, 487, 973
MroNI GCCGGC 1 cut(s) 1376
MroXI GAANNNNTTC 1 cut(s) 1078
MseI TTAA 2 cut(s) 372, 444
MspR9I CCNGG 3 cut(s) 180, 203, 259
MvaI CCWGG 2 cut(s) 180, 203
MvnI CGCG 1 cut(s) 1482
MwoI GCNNNNNNNGC 4 cut(s) 195, 248, 1229, 1238
NaeI GCCGGC 1 cut(s) 1378
NciI CCSGG 1 cut(s) 259
NdeI CATATG 1 cut(s) 1228
NdeII GATC 3 cut(s) 645, 892, 1140
NgoMIV GCCGGC 1 cut(s) 1376
NlaIII CATG 7 cut(s) 554, 705, 899, 1020, 1210, 1304, 1400
NlaIV GGNNCC 4 cut(s) 232, 384, 780, 997
NmuCI GTSAC 3 cut(s) 24, 87, 795
NspI RCATGY 1 cut(s) 1400
PagI TCATGA 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 1316
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 1 cut(s) 1378
PdmI GAANNNNTTC 1 cut(s) 1078
PfeI GAWTC 9 cut(s) 39, 60, 81, 254, 325, 475, 875, 986, 1007
PflFI GACNNNGTC 1 cut(s) 297
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 9 cut(s) 34, 197, 244, 247, 250, 340, 1059, 1270, 1293
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 307, 1328
Psp6I CCWGG 2 cut(s) 178, 201
PspGI CCWGG 2 cut(s) 178, 201
PspN4I GGNNCC 4 cut(s) 232, 384, 780, 997
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 2 cut(s) 250, 1273
PstNI CAGNNNCTG 1 cut(s) 1493
PsuI RGATCY 2 cut(s) 645, 1140
PsyI GACNNNGTC 1 cut(s) 297
RsaI GTAC 2 cut(s) 384, 1275
RsaNI GTAC 2 cut(s) 383, 1274
SacI GAGCTC 2 cut(s) 307, 1328
SapI GCTCTTC 1 cut(s) 1316
SaqAI TTAA 2 cut(s) 372, 444
SatI GCNGC 9 cut(s) 33, 196, 243, 246, 249, 339, 1058, 1269, 1292
Sau3AI GATC 3 cut(s) 645, 892, 1140
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 3 cut(s) 180, 203, 259
SduI GDGCHC 3 cut(s) 191, 307, 1328
SfaNI GCATC 4 cut(s) 1058, 1411, 1435, 1480
SfcI CTRYAG 2 cut(s) 246, 1269
SgrAI CRCCGGYG 2 cut(s) 191, 309
SmlI CTYRAG 4 cut(s) 641, 788, 951, 1021
SmoI CTYRAG 4 cut(s) 641, 788, 951, 1021
Sse9I AATT 8 cut(s) 109, 594, 862, 1186, 1249, 1388, 1405, 1453
SsiI CCGC 6 cut(s) 33, 195, 274, 848, 1289, 1480
SspI AATATT 1 cut(s) 1476
SspMI CTAG 8 cut(s) 351, 410, 518, 738, 1083, 1128, 1296, 1504
SstI GAGCTC 2 cut(s) 307, 1328
StyD4I CCNGG 3 cut(s) 178, 201, 257
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 1030, 1114
TaqI TCGA 4 cut(s) 185, 213, 301, 1052
TaqII GACCGA 1 cut(s) 287
TasI AATT 8 cut(s) 109, 594, 862, 1186, 1249, 1388, 1405, 1453
TatI WGTACW 1 cut(s) 1273
TauI GCSGC 2 cut(s) 35, 198
TfiI GAWTC 9 cut(s) 39, 60, 81, 254, 325, 475, 875, 986, 1007
Tru1I TTAA 2 cut(s) 372, 444
Tru9I TTAA 2 cut(s) 372, 444
TscAI CASTG 2 cut(s) 100, 1035
TseFI GTSAC 3 cut(s) 24, 87, 795
TseI GCWGC 7 cut(s) 242, 245, 248, 338, 1057, 1268, 1291
Tsp45I GTSAC 3 cut(s) 24, 87, 795
TspDTI ATGAA 8 cut(s) 633, 690, 818, 900, 936, 945, 972, 1195
TspGWI ACGGA 4 cut(s) 337, 515, 1273, 1457
TspRI CASTG 2 cut(s) 100, 1035
Tth111I GACNNNGTC 1 cut(s) 297
XagI CCTNNNNNAGG 1 cut(s) 171
XapI RAATTY 3 cut(s) 109, 1249, 1388
XbaI TCTAGA 1 cut(s) 1127
XceI RCATGY 1 cut(s) 1400
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XmaJI CCTAGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 1078
XspI CTAG 8 cut(s) 351, 410, 518, 738, 1083, 1128, 1296, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.