RLG00000017234

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000593
Physical Location & Seq
Reverse (-)
14404047 .. 14407054
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017234

Sequence Viewer

Length: 1212 bp
ATGGCAACGGAAAGAAACCCCTCGTTTTTCAAGGTTCTTCAGGGTGATCAGTTCTCTATCAAGTTGAAAATCCCTAGAGCATTTCTCCAGTATTTTAATGGAAAGGTACCTCCCAAGTGCTTTCTTCAAACACTAGGTCAAACTTGGCTTGTTGATGTGGAAAAGGTTAATCACAAGTTCTATTTCCAACTGGGGTGGAATCGCTTTGTGCAAGACAATGGCTTGAAGTATGGTCAACTTCTAGTCTTCCGTTATGCTGGAAACTCAGACTTCCATGTGGACATATTTGGAATAAATACCTGCAAAAATCTGTGTGTAATTCCTGAGATGGAAGAAGCTAATGAACATGATGATGATGTATCTCTTGAGATCTTGGACGACTTTCCACCCTGCCAAAGAAGAACAGAGGAGAAGTCTTTGCTTTCAAGTCCTCTGCCTCGCAAGAAAAACAAAACAAGTTCTAGTGGTAGAGCAGATATCACCCCCAACTTTCAAAAGAGTGTTCCACATCTTGGGAAGTCAGAAATGAACAGGAAAGAGGCACATTGGTTCAAAACAGAAATGATGAATAATTCTGATACTGATTTTGGTGATGAAGATGATCATGATGAGGAGGATGACAACGAAAATGAAGATGATGAAGAAGAAGAAGAAACTCAAGATGAAGAGGTTGAGGAGGAAGATGATGGTGATTCTGTTGGAACCCTCAACGATTCTCTACCATGCTCAAGAACAGTGGGGAAATATACATTAGCATCGAGGCTGCACCAGAGAGAGAGAAAGAGTTCTAGTGCAAAAGCAAAAACAAACATCAAAACTGTTGATGGCTTGTCTAGAGAGGAAAAGATCCTAAAAGTTATAGCTCTACAAAGAGCTAATGCTTTCAAATCTAATTCCGTCAACCCTTGTGTCATGGTTTCCTTGTCTGCCACATATGCTCGTGGAGCTTTTCTGAATTTGCCACGGAGTTTTGCTGCAATACATTTTACTAATCCGCGGCCTGGGCACGTCATCCTTTGGGTTGAGAAGAGCTCTTGGTTTGTCAAGGTCAGTTATATTGGGACTGCAACGAGATTACAGGCCGGCTGGTCTGAATTTGTGCATGCCAATAATTTGAAGATTGGTGATGCTTGTGTTTTTGTCTTGACGGATGCCATCAATTTTCAATATGATGTTGAAATATTCCGCGTTACAGATGCTGTTTGCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

404

Amino Acids

45.89

Weight (kDa)

5.18

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 9 - 96 5.7e-14 B3 DNA binding domain
B3 PF02362 314 - 397 8.1e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 308
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 106
AccB7I CCANNNNNTGG 1 cut(s) 512
AccII CGCG 2 cut(s) 997, 1188
AciI CCGC 3 cut(s) 995, 997, 1186
AclWI GGATC 1 cut(s) 841
AcsI RAATTY 2 cut(s) 955, 1094
AcuI CTGAAG 1 cut(s) 23
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 512, 1001
AjiI CACGTC 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 1000
AluBI AGCT 5 cut(s) 338, 863, 875, 947, 1032
AluI AGCT 5 cut(s) 338, 863, 875, 947, 1032
Alw21I GWGCWC 1 cut(s) 1034
AlwI GGATC 1 cut(s) 841
AlwNI CAGNNNCTG 1 cut(s) 1199
AoxI GGCC 2 cut(s) 998, 1080
ApeKI GCWGC 2 cut(s) 763, 974
ApoI RAATTY 2 cut(s) 955, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
Asp700I GAANNNNTTC 1 cut(s) 784
Asp718I GGTACC 1 cut(s) 106
AsuHPI GGTGA 5 cut(s) 56, 472, 602, 701, 1136
BaeGI GKGCMC 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 1034
BauI CACGAG 1 cut(s) 939
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 1034
BbvI GCAGC 2 cut(s) 750, 961
BccI CCATC 4 cut(s) 322, 680, 818, 1163
BciT130I CCWGG 1 cut(s) 1002
BclI TGATCA 2 cut(s) 46, 601
BfaI CTAG 7 cut(s) 75, 134, 242, 462, 789, 834, 1210
BfuAI ACCTGC 1 cut(s) 308
BglII AGATCT 1 cut(s) 369
BisI GCNGC 3 cut(s) 764, 975, 998
BlsI GCNGC 3 cut(s) 765, 976, 999
Bme1390I CCNGG 1 cut(s) 1002
BmgBI CACGTC 1 cut(s) 1009
BmiI GGNNCC 2 cut(s) 108, 703
BmrFI CCNGG 1 cut(s) 1002
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 4 cut(s) 764, 1117, 1141, 1186
BmuI ACTGGG 1 cut(s) 200
BpiI GAAGAC 1 cut(s) 238
BplI GAGNNNNNCTC 4 cut(s) 69, 101, 1016, 1048
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 3 cut(s) 386, 642, 712
BsaJI CCNNGG 3 cut(s) 962, 995, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 1001
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 2 cut(s) 88, 195
BseBI CCWGG 1 cut(s) 1002
BseDI CCNNGG 3 cut(s) 962, 995, 1001
BseGI GGATG 3 cut(s) 622, 1011, 1156
BseLI CCNNNNNNNGG 2 cut(s) 512, 1001
BseMII CTCAG 2 cut(s) 279, 315
BseNI ACTGG 2 cut(s) 88, 195
BseRI GAGGAG 3 cut(s) 422, 626, 689
BseSI GKGCMC 1 cut(s) 1008
BseXI GCAGC 2 cut(s) 750, 961
BsgI GTGCAG 1 cut(s) 749
Bsh1236I CGCG 2 cut(s) 997, 1188
BshFI GGCC 2 cut(s) 1000, 1082
BshNI GGYRCC 1 cut(s) 106
BsiHKAI GWGCWC 1 cut(s) 1034
BsiSI CCGG 1 cut(s) 1083
BslFI GGGAC 1 cut(s) 1075
BslI CCNNNNNNNGG 2 cut(s) 512, 1001
BsmFI GGGAC 1 cut(s) 1075
BsnI GGCC 2 cut(s) 1000, 1082
Bsp1286I GDGCHC 2 cut(s) 1008, 1034
Bsp143I GATC 4 cut(s) 46, 369, 601, 846
BspACI CCGC 3 cut(s) 995, 997, 1186
BspANI GGCC 2 cut(s) 1000, 1082
BspCNI CTCAG 2 cut(s) 278, 316
BspFNI CGCG 2 cut(s) 997, 1188
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 108, 703
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 1 cut(s) 841
BspQI GCTCTTC 1 cut(s) 1022
BspT107I GGYRCC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 1082
BsrI ACTGG 2 cut(s) 88, 195
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 3 cut(s) 962, 995, 1001
BssMI GATC 4 cut(s) 46, 369, 601, 846
BssSI CACGAG 1 cut(s) 939
Bst2BI CACGAG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 1002
Bst4CI ACNGT 2 cut(s) 736, 820
Bst6I CTCTTC 2 cut(s) 660, 1022
BstC8I GCNNGC 2 cut(s) 1084, 1104
BstDEI CTNAG 2 cut(s) 265, 324
BstDSI CCRYGG 2 cut(s) 962, 995
BstF5I GGATG 3 cut(s) 622, 1011, 1156
BstFNI CGCG 2 cut(s) 997, 1188
BstKTI GATC 4 cut(s) 49, 372, 604, 849
BstMBI GATC 4 cut(s) 46, 369, 601, 846
BstMWI GCNNNNNNNGC 3 cut(s) 935, 944, 1003
BstNI CCWGG 1 cut(s) 1002
BstNSI RCATGY 1 cut(s) 1106
BstSCI CCNGG 1 cut(s) 1000
BstSLI GKGCMC 1 cut(s) 1008
BstUI CGCG 2 cut(s) 997, 1188
BstV1I GCAGC 2 cut(s) 750, 961
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 369, 846
BstYI RGATCY 2 cut(s) 369, 846
BsuRI GGCC 2 cut(s) 1000, 1082
BtgI CCRYGG 2 cut(s) 962, 995
BtrI CACGTC 1 cut(s) 1009
BtsCI GGATG 3 cut(s) 622, 1011, 1156
BtsIMutI CAGTG 1 cut(s) 741
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 2 cut(s) 1084, 1104
CaiI CAGNNNCTG 1 cut(s) 1199
CciI TCATGA 1 cut(s) 604
Cfr10I RCCGGY 1 cut(s) 1082
Cfr42I CCGCGG 1 cut(s) 998
Csp6I GTAC 1 cut(s) 107
CspCI CAANNNNNGTGG 4 cut(s) 176, 211, 717, 752
CviAII CATG 6 cut(s) 275, 347, 605, 723, 913, 1103
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 265, 324
DpnI GATC 4 cut(s) 48, 371, 603, 848
DpnII GATC 4 cut(s) 46, 369, 601, 846
Eam1104I CTCTTC 2 cut(s) 660, 1022
EarI CTCTTC 2 cut(s) 660, 1022
Ecl136II GAGCTC 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 1034
Eco32I GATATC 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 1032
Eco57I CTGAAG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 1032
EcoRII CCWGG 1 cut(s) 1000
EcoRV GATATC 1 cut(s) 478
EcoT38I GRGCYC 1 cut(s) 1034
FaeI CATG 6 cut(s) 278, 350, 608, 726, 916, 1106
FaqI GGGAC 1 cut(s) 1075
FatI CATG 6 cut(s) 274, 346, 604, 722, 912, 1102
FauNDI CATATG 1 cut(s) 934
FbaI TGATCA 2 cut(s) 46, 601
Fnu4HI GCNGC 3 cut(s) 764, 975, 998
FokI GGATG 3 cut(s) 629, 998, 1163
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 3 cut(s) 764, 975, 998
FspBI CTAG 7 cut(s) 75, 134, 242, 462, 789, 834, 1210
GluI GCNGC 3 cut(s) 764, 975, 998
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 1000, 1082
HapII CCGG 1 cut(s) 1083
Hin1II CATG 6 cut(s) 278, 350, 608, 726, 916, 1106
HincII GTYRAC 2 cut(s) 236, 901
HindII GTYRAC 2 cut(s) 236, 901
HinfI GANTC 3 cut(s) 199, 692, 713
HpaII CCGG 1 cut(s) 1083
HphI GGTGA 5 cut(s) 56, 472, 602, 701, 1136
Hpy166II GTNNAC 3 cut(s) 236, 280, 901
Hpy188I TCNGA 5 cut(s) 268, 523, 577, 954, 1093
Hpy188III TCNNGA 7 cut(s) 323, 365, 605, 659, 729, 834, 1144
Hpy8I GTNNAC 3 cut(s) 236, 280, 901
HpyCH4III ACNGT 2 cut(s) 736, 820
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 8 cut(s) 211, 303, 766, 794, 977, 1067, 1102, 1206
HpyF10VI GCNNNNNNNGC 3 cut(s) 935, 944, 1003
HpyF3I CTNAG 2 cut(s) 265, 324
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 6 cut(s) 278, 350, 608, 726, 916, 1106
KpnI GGTACC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 1082
KroNI GCCGGC 1 cut(s) 1084
Ksp22I TGATCA 2 cut(s) 46, 601
KspI CCGCGG 1 cut(s) 998
Kzo9I GATC 4 cut(s) 46, 369, 601, 846
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 1 cut(s) 944
Lsp1109I GCAGC 2 cut(s) 750, 961
LweI GCATC 4 cut(s) 764, 1117, 1141, 1186
MaeI CTAG 7 cut(s) 75, 134, 242, 462, 789, 834, 1210
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 1 cut(s) 1189
MalI GATC 4 cut(s) 48, 371, 603, 848
MboI GATC 4 cut(s) 46, 369, 601, 846
MflI RGATCY 2 cut(s) 369, 846
MhlI GDGCHC 2 cut(s) 1008, 1034
MluCI AATT 7 cut(s) 318, 571, 892, 955, 1094, 1111, 1159
MmeI TCCRAC 2 cut(s) 211, 679
MroNI GCCGGC 1 cut(s) 1082
MroXI GAANNNNTTC 1 cut(s) 784
MseI TTAA 2 cut(s) 96, 168
MslI CAYNNNNRTG 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 997
MspI CCGG 1 cut(s) 1083
MspR9I CCNGG 1 cut(s) 1002
MvaI CCWGG 1 cut(s) 1002
MvnI CGCG 2 cut(s) 997, 1188
MwoI GCNNNNNNNGC 3 cut(s) 935, 944, 1003
NaeI GCCGGC 1 cut(s) 1084
NdeI CATATG 1 cut(s) 934
NdeII GATC 4 cut(s) 46, 369, 601, 846
NgoMIV GCCGGC 1 cut(s) 1082
NlaIII CATG 6 cut(s) 278, 350, 608, 726, 916, 1106
NlaIV GGNNCC 2 cut(s) 108, 703
NspI RCATGY 1 cut(s) 1106
PaeI GCATGC 1 cut(s) 1106
PagI TCATGA 1 cut(s) 604
PciSI GCTCTTC 1 cut(s) 1022
PdiI GCCGGC 1 cut(s) 1084
PdmI GAANNNNTTC 1 cut(s) 784
PfeI GAWTC 3 cut(s) 199, 692, 713
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 3 cut(s) 765, 976, 999
Psp124BI GAGCTC 1 cut(s) 1034
Psp6I CCWGG 1 cut(s) 1000
PspGI CCWGG 1 cut(s) 1000
PspN4I GGNNCC 2 cut(s) 108, 703
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 369, 846
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 351
SacI GAGCTC 1 cut(s) 1034
SacII CCGCGG 1 cut(s) 998
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 2 cut(s) 96, 168
SatI GCNGC 3 cut(s) 764, 975, 998
Sau3AI GATC 4 cut(s) 46, 369, 601, 846
ScrFI CCNGG 1 cut(s) 1002
SduI GDGCHC 2 cut(s) 1008, 1034
SfaNI GCATC 4 cut(s) 764, 1117, 1141, 1186
Sfr303I CCGCGG 1 cut(s) 998
SgrBI CCGCGG 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 3 cut(s) 365, 657, 727
SmoI CTYRAG 3 cut(s) 365, 657, 727
SphI GCATGC 1 cut(s) 1106
Sse9I AATT 7 cut(s) 318, 571, 892, 955, 1094, 1111, 1159
SsiI CCGC 3 cut(s) 995, 997, 1186
SspI AATATT 1 cut(s) 1182
SspMI CTAG 7 cut(s) 75, 134, 242, 462, 789, 834, 1210
SstI GAGCTC 1 cut(s) 1034
StyD4I CCNGG 1 cut(s) 1000
TaaI ACNGT 2 cut(s) 736, 820
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 1 cut(s) 758
TasI AATT 7 cut(s) 318, 571, 892, 955, 1094, 1111, 1159
TauI GCSGC 1 cut(s) 1000
TfiI GAWTC 3 cut(s) 199, 692, 713
Tru1I TTAA 2 cut(s) 96, 168
Tru9I TTAA 2 cut(s) 96, 168
TscAI CASTG 1 cut(s) 741
TseI GCWGC 2 cut(s) 763, 974
TspDTI ATGAA 7 cut(s) 357, 542, 581, 609, 645, 654, 678
TspGWI ACGGA 5 cut(s) 23, 239, 886, 979, 1163
TspRI CASTG 1 cut(s) 741
Van91I CCANNNNNTGG 1 cut(s) 512
XapI RAATTY 2 cut(s) 955, 1094
XbaI TCTAGA 1 cut(s) 833
XceI RCATGY 1 cut(s) 1106
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 784
XspI CTAG 7 cut(s) 75, 134, 242, 462, 789, 834, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.