FvH4_4g04150

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000605
Physical Location & Seq
Forward (+)
3584472 .. 3587714
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04150.t1

Sequence Viewer

Length: 1182 bp
ATGGTGGAAGGTGAAGCTCTCGTCAATGAACTCGCCACCAAGCTCAGGAAACGGAAGGTGGTGGGTTCTAGAGAGACTGCTCGGCTGACGGCGGAGTTGTTCCGGACGGTGATATCCCTGCAGAGGGTGACGTCAGCCGCCCTTGTTGAGGTAGTCAGAGATGTTGGCCAGAAGCTTATTGCTGCCAACCCTGTTGAGCTTGCTGTTGGGAACATTGTTAGGCGTGTTCTGCGCATCATTAGGGACAGTGATCTTTCACTGGAAGTTGAAGGATTAACTTTAGTCTCTCAAGGTGATGATGGAGATGTTGTTGAGGAAGATGATAAACCATTTCGATTATCTGATGCTATTTCCCAAACAATTTTCCATCCGCCTTCATTGGTGGCACAGCTTGAAAGCAAACCCAACTTGGTTCGCCACTTTGAGGGAAAAGCTGATAAAACTCCTAGGAGCTGGCAGTTTAAGCACCACATCATTGAGGGAGTTAATGATCTCATTGAGGATATAAATACTTGCCATGAACTGATCGCAGACCAGGCGTTGGAGCTTATACATCAAAATGAGGTGATACTAACATTAGGTCATTCAAGGACAGTGAAGAACTTCCTCTATGCTGCAAAGGAGAAGAAAAGATCGTTCCATGTCTATGTGGCCGAGGGTGCTCCTAAGCATTTGGGGCATGTGCTTGCAAATGAGTTGGCTGAAAAGCATTTGCGAACAACCGTTATTCCAGACTCTTCAGTGTTTGCCATGATTTCAAGAGTTAACATGGTTATAGTCGGGGTTCATGCTGTGATGGCCAACGGTGGGGTCATTGCGCCTGTTGGGATGAATATGGTTGCACTTGCTGCGAAAAGGCACGCTGTTCCATTTGTTGTGGTTGCTGGCACTCATAAGTTATGTCCCCTATTTCCAAACAATCCAGAGGTTTTGCTGAATGATATGAGATGTCCATCCGAGCTATTACCTTTTGGGGATTTCTCAGATTGCATGGATTATAGTGTTGGTAGTGGAGCTCCTCATTTGCATGTTGTTAATCCTGCATTTGATTATGTGCCTCCCGAGCTAGTCAGTCTTTTTGTTACTGAACTAGGGGGTTATCATCCATCGTACATCTATAGGCTCATTGCGGATTATTACTCTGATGATGATATAGTTCTGCAACAAAAACCTGCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IF-2B

Protein Analysis

394

Amino Acids

43.37

Weight (kDa)

5.93

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 19 - 369 1.7e-64 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
Acc16I TGCGCA 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 541
AccIII TCCGGA 1 cut(s) 102
AciI CCGC 4 cut(s) 92, 138, 371, 1130
AcoI YGGCCR 3 cut(s) 166, 651, 798
AcuI CTGAAG 1 cut(s) 723
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 1 cut(s) 1112
AfiI CCNNNNNNNGG 7 cut(s) 45, 123, 124, 148, 424, 541, 807
AgsI TTSAA 4 cut(s) 269, 395, 588, 759
AjnI CCWGG 1 cut(s) 534
Alw21I GWGCWC 2 cut(s) 664, 1018
Alw26I GTCTC 2 cut(s) 68, 289
Ama87I CYCGRG 1 cut(s) 1061
Aor13HI TCCGGA 1 cut(s) 102
AoxI GGCC 3 cut(s) 166, 651, 798
ApeKI GCWGC 3 cut(s) 182, 614, 848
Asp700I GAANNNNTTC 1 cut(s) 602
AspA2I CCTAGG 1 cut(s) 446
AspLEI GCGC 2 cut(s) 234, 820
AsuHPI GGTGA 5 cut(s) 23, 121, 139, 305, 577
AvaI CYCGRG 1 cut(s) 1061
AvrII CCTAGG 1 cut(s) 446
BalI TGGCCA 2 cut(s) 168, 800
BanII GRGCYC 1 cut(s) 1018
Bbv12I GWGCWC 2 cut(s) 664, 1018
BbvI GCAGC 3 cut(s) 169, 601, 835
BccI CCATC 5 cut(s) 293, 375, 790, 961, 1114
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 2 cut(s) 68, 289
BfaI CTAG 4 cut(s) 69, 447, 1067, 1091
BfmI CTRYAG 2 cut(s) 119, 1117
BisI GCNGC 4 cut(s) 138, 183, 615, 849
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 4 cut(s) 139, 184, 616, 850
Bme1390I CCNGG 1 cut(s) 536
BmeT110I CYCGRG 1 cut(s) 1061
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 243, 334
Bpu10I CCTNAGC 2 cut(s) 44, 666
BpuEI CTTGAG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 952
BsaHI GRCGYC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 446, 654
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 7 cut(s) 45, 123, 124, 148, 424, 541, 807
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 2 cut(s) 813, 1125
Bse8I GATNNNNATC 1 cut(s) 952
BseAI TCCGGA 1 cut(s) 102
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 446, 654
BseGI GGATG 4 cut(s) 367, 834, 953, 1102
BseJI GATNNNNATC 1 cut(s) 952
BseLI CCNNNNNNNGG 7 cut(s) 45, 123, 124, 148, 424, 541, 807
BseMI GCAATG 2 cut(s) 813, 1125
BseMII CTCAG 2 cut(s) 58, 996
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 1008
BseXI GCAGC 3 cut(s) 169, 601, 835
BshFI GGCC 3 cut(s) 168, 653, 800
BsiHKAI GWGCWC 2 cut(s) 664, 1018
BsiHKCI CYCGRG 1 cut(s) 1061
BsiSI CCGG 1 cut(s) 103
BslFI GGGAC 2 cut(s) 257, 888
BslI CCNNNNNNNGG 7 cut(s) 45, 123, 124, 148, 424, 541, 807
BsmAI GTCTC 2 cut(s) 68, 289
BsmFI GGGAC 2 cut(s) 257, 888
BsnI GGCC 3 cut(s) 168, 653, 800
BsoBI CYCGRG 1 cut(s) 1061
Bsp1286I GDGCHC 2 cut(s) 664, 1018
Bsp13I TCCGGA 1 cut(s) 102
Bsp143I GATC 4 cut(s) 250, 490, 525, 632
BspACI CCGC 4 cut(s) 92, 138, 371, 1130
BspANI GGCC 3 cut(s) 168, 653, 800
BspCNI CTCAG 2 cut(s) 57, 995
BspEI TCCGGA 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 123
BsrDI GCAATG 2 cut(s) 813, 1125
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 2 cut(s) 446, 654
BssMI GATC 4 cut(s) 250, 490, 525, 632
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 5 cut(s) 109, 248, 595, 724, 806
Bst6I CTCTTC 1 cut(s) 742
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 848
BstC8I GCNNGC 6 cut(s) 201, 455, 687, 861, 886, 1177
BstDEI CTNAG 3 cut(s) 44, 666, 982
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 4 cut(s) 367, 834, 953, 1102
BstHHI GCGC 2 cut(s) 234, 820
BstKTI GATC 4 cut(s) 253, 493, 528, 635
BstMAI GTCTC 2 cut(s) 68, 289
BstMBI GATC 4 cut(s) 250, 490, 525, 632
BstMWI GCNNNNNNNGC 8 cut(s) 229, 463, 536, 659, 676, 797, 848, 1063
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 2 cut(s) 683, 1031
BstSCI CCNGG 1 cut(s) 534
BstSFI CTRYAG 2 cut(s) 119, 1117
BstV1I GCAGC 3 cut(s) 169, 601, 835
BsuRI GGCC 3 cut(s) 168, 653, 800
BtsCI GGATG 4 cut(s) 367, 834, 953, 1102
BtsIMutI CAGTG 4 cut(s) 253, 257, 600, 747
Cac8I GCNNGC 6 cut(s) 201, 455, 687, 861, 886, 1177
CfoI GCGC 2 cut(s) 234, 820
Csp6I GTAC 1 cut(s) 1111
CviAII CATG 8 cut(s) 518, 641, 680, 751, 769, 788, 991, 1028
CviQI GTAC 1 cut(s) 1111
DdeI CTNAG 3 cut(s) 44, 666, 982
DpnI GATC 4 cut(s) 252, 492, 527, 634
DpnII GATC 4 cut(s) 250, 490, 525, 632
EaeI YGGCCR 3 cut(s) 166, 651, 798
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
EciI GGCGGA 2 cut(s) 107, 360
Ecl136II GAGCTC 1 cut(s) 1016
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 1018
Eco32I GATATC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 1 cut(s) 723
Eco88I CYCGRG 1 cut(s) 1061
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 1018
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 8 cut(s) 521, 644, 683, 754, 772, 791, 994, 1031
FaqI GGGAC 2 cut(s) 257, 888
FatI CATG 8 cut(s) 517, 640, 679, 750, 768, 787, 990, 1027
Fnu4HI GCNGC 4 cut(s) 138, 183, 615, 849
FokI GGATG 4 cut(s) 354, 841, 940, 1089
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 4 cut(s) 138, 183, 615, 849
FspBI CTAG 4 cut(s) 69, 447, 1067, 1091
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 2 cut(s) 233, 819
GluI GCNGC 4 cut(s) 138, 183, 615, 849
HaeIII GGCC 3 cut(s) 168, 653, 800
HapII CCGG 1 cut(s) 103
HhaI GCGC 2 cut(s) 234, 820
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 8 cut(s) 521, 644, 683, 754, 772, 791, 994, 1031
Hin6I GCGC 2 cut(s) 232, 818
HinP1I GCGC 2 cut(s) 232, 818
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 734
HpaI GTTAAC 1 cut(s) 766
HpaII CCGG 1 cut(s) 103
HphI GGTGA 5 cut(s) 23, 121, 139, 305, 577
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 5 cut(s) 158, 343, 958, 985, 1144
Hpy188III TCNNGA 7 cut(s) 46, 69, 103, 731, 759, 923, 1061
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 3 cut(s) 49, 263, 384
HpyCH4III ACNGT 5 cut(s) 109, 248, 595, 724, 806
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 8 cut(s) 121, 617, 689, 842, 990, 1027, 1043, 1162
HpyF10VI GCNNNNNNNGC 8 cut(s) 229, 463, 536, 659, 676, 797, 848, 1063
HpyF3I CTNAG 3 cut(s) 44, 666, 982
HpySE526I ACGT 1 cut(s) 131
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 8 cut(s) 521, 644, 683, 754, 772, 791, 994, 1031
HspAI GCGC 2 cut(s) 232, 818
Kpn2I TCCGGA 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 766
Kzo9I GATC 4 cut(s) 250, 490, 525, 632
LmnI GCTCC 5 cut(s) 450, 544, 667, 1013, 1021
Lsp1109I GCAGC 3 cut(s) 169, 601, 835
LweI GCATC 2 cut(s) 243, 334
MaeI CTAG 4 cut(s) 69, 447, 1067, 1091
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 127, 1081
MalI GATC 4 cut(s) 252, 492, 527, 634
MboI GATC 4 cut(s) 250, 490, 525, 632
MboII GAAGA 4 cut(s) 329, 610, 637, 729
MhlI GDGCHC 2 cut(s) 664, 1018
MlsI TGGCCA 2 cut(s) 168, 800
MluCI AATT 1 cut(s) 360
MluNI TGGCCA 2 cut(s) 168, 800
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 1 cut(s) 522
Mox20I TGGCCA 2 cut(s) 168, 800
MroI TCCGGA 1 cut(s) 102
MroXI GAANNNNTTC 1 cut(s) 602
MscI TGGCCA 2 cut(s) 168, 800
MseI TTAA 5 cut(s) 275, 462, 486, 765, 1035
MslI CAYNNNNRTG 3 cut(s) 558, 645, 1026
Msp20I TGGCCA 2 cut(s) 168, 800
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 536
MwoI GCNNNNNNNGC 8 cut(s) 229, 463, 536, 659, 676, 797, 848, 1063
NdeII GATC 4 cut(s) 250, 490, 525, 632
NlaIII CATG 8 cut(s) 521, 644, 683, 754, 772, 791, 994, 1031
NmeAIII GCCGAG 2 cut(s) 61, 679
NmuCI GTSAC 1 cut(s) 127
NsbI TGCGCA 1 cut(s) 233
NspI RCATGY 2 cut(s) 683, 1031
PdmI GAANNNNTTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 4 cut(s) 139, 184, 616, 850
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
Psp124BI GAGCTC 1 cut(s) 1018
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PstI CTGCAG 1 cut(s) 123
RsaI GTAC 1 cut(s) 1112
RsaNI GTAC 1 cut(s) 1111
RseI CAYNNNNRTG 3 cut(s) 558, 645, 1026
SacI GAGCTC 1 cut(s) 1018
SaqAI TTAA 5 cut(s) 275, 462, 486, 765, 1035
SatI GCNGC 4 cut(s) 138, 183, 615, 849
Sau3AI GATC 4 cut(s) 250, 490, 525, 632
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 2 cut(s) 664, 1018
SfaNI GCATC 2 cut(s) 243, 334
SfcI CTRYAG 2 cut(s) 119, 1117
SmiMI CAYNNNNRTG 3 cut(s) 558, 645, 1026
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 1 cut(s) 360
SsiI CCGC 4 cut(s) 92, 138, 371, 1130
SspMI CTAG 4 cut(s) 69, 447, 1067, 1091
SstI GAGCTC 1 cut(s) 1018
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 5 cut(s) 109, 248, 595, 724, 806
TaiI ACGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 334
TasI AATT 1 cut(s) 360
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 5 cut(s) 275, 462, 486, 765, 1035
Tru9I TTAA 5 cut(s) 275, 462, 486, 765, 1035
TscAI CASTG 4 cut(s) 253, 264, 600, 747
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 3 cut(s) 182, 614, 848
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 5 cut(s) 42, 366, 534, 776, 845
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 4 cut(s) 253, 264, 600, 747
Van91I CCANNNNNTGG 1 cut(s) 541
XagI CCTNNNNNAGG 1 cut(s) 146
XbaI TCTAGA 1 cut(s) 68
XceI RCATGY 2 cut(s) 683, 1031
XmaJI CCTAGG 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 4 cut(s) 69, 447, 1067, 1091
ZraI GACGTC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.