RchiOBHm_Chr1g0378031

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000605
Physical Location & Seq
Reverse (-)
64656045 .. 64661632
5588 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60146

Sequence Viewer

Length: 387 bp
ATGGGGTGTCTTCTCCATCCATCAGAATACCTAGATTTATGTTATATTAACTCTAGGCAATTCAAAAACAGTACAGGAATTCCTTTTTGCTGCAAAGGAGAAAAAAAAAGATCATTTCGGGTATTTGTTGCAGAGGGAGCTCCAAGGTATCAGGGGCATCTTCTTGCAAAAGAATTGGCTGGAAGAGGTTTACAAACAGCGCTGATTACTGATTCTGCAATTTTTGCTATGATATCTCAAGTGATCATGGTTATAGTTGGTGCTCATGCTGTCATGGCCAATGGTGGTGTTATAGCACCTGTTGGGTTGAATATGGTTGCACTTGCAGCCCAAAGGCATGCTGTCCCTTTTGTTGTACTTGCTGGCAGTTACAAGTGGAGGCTATAA

Protein Analysis

128

Amino Acids

13.85

Weight (kDa)

9.59

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 34 - 125 1.2e-29 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 1 cut(s) 78
AfaI GTAC 2 cut(s) 73, 357
AfeI AGCGCT 1 cut(s) 201
AgsI TTSAA 2 cut(s) 64, 310
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw21I GWGCWC 2 cut(s) 142, 265
Aor51HI AGCGCT 1 cut(s) 201
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 90, 326
ApoI RAATTY 1 cut(s) 78
AspLEI GCGC 1 cut(s) 202
BalI TGGCCA 1 cut(s) 278
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 2 cut(s) 142, 265
BbvI GCAGC 2 cut(s) 77, 338
BccI CCATC 2 cut(s) 24, 28
BclI TGATCA 1 cut(s) 243
BfaI CTAG 2 cut(s) 32, 54
BfoI RGCGCY 1 cut(s) 203
BisI GCNGC 2 cut(s) 91, 327
BlsI GCNGC 2 cut(s) 92, 328
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 1 cut(s) 2
BpuEI CTTGAG 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 1 cut(s) 16
BseXI GCAGC 2 cut(s) 77, 338
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 142, 265
BslFI GGGAC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 142, 265
Bsp143I GATC 2 cut(s) 110, 243
BspANI GGCC 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 2 cut(s) 110, 243
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 224
BstC8I GCNNGC 2 cut(s) 339, 364
BstF5I GGATG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 113, 246
BstMBI GATC 2 cut(s) 110, 243
BstMWI GCNNNNNNNGC 4 cut(s) 137, 224, 275, 326
BstNSI RCATGY 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 77, 338
BstV2I GAAGAC 1 cut(s) 2
BsuRI GGCC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 339, 364
CfoI GCGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 72, 356
CviAII CATG 4 cut(s) 247, 266, 274, 338
CviJI RGCY 5 cut(s) 140, 179, 278, 329, 382
CviKI_1 RGCY 5 cut(s) 140, 179, 278, 329, 382
CviQI GTAC 2 cut(s) 72, 356
DpnI GATC 2 cut(s) 112, 245
DpnII GATC 2 cut(s) 110, 243
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 142
Eco32I GATATC 1 cut(s) 234
Eco47III AGCGCT 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 78
EcoRV GATATC 1 cut(s) 234
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 4 cut(s) 250, 269, 277, 341
FaqI GGGAC 1 cut(s) 329
FatI CATG 4 cut(s) 246, 265, 273, 337
FbaI TGATCA 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 91, 327
FokI GGATG 1 cut(s) 3
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 2 cut(s) 91, 327
FspBI CTAG 2 cut(s) 32, 54
GlaI GCGC 1 cut(s) 201
GluI GCNGC 2 cut(s) 91, 327
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 1 cut(s) 278
HhaI GCGC 1 cut(s) 202
Hin1II CATG 4 cut(s) 250, 269, 277, 341
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 1 cut(s) 25
Hpy8I GTNNAC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 6 cut(s) 93, 131, 167, 218, 320, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 224, 275, 326
Hsp92II CATG 4 cut(s) 250, 269, 277, 341
HspAI GCGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 2 cut(s) 110, 243
LmnI GCTCC 2 cut(s) 137, 145
LpnPI CCDG 5 cut(s) 60, 137, 165, 312, 348
Lsp1109I GCAGC 2 cut(s) 77, 338
LweI GCATC 1 cut(s) 166
MaeI CTAG 2 cut(s) 32, 54
MaeIII GTNAC 1 cut(s) 368
MalI GATC 2 cut(s) 112, 245
MboI GATC 2 cut(s) 110, 243
MboII GAAGA 2 cut(s) 152, 195
MhlI GDGCHC 2 cut(s) 142, 265
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 4 cut(s) 59, 78, 173, 219
MluNI TGGCCA 1 cut(s) 278
MnlI CCTC 3 cut(s) 127, 179, 372
Mox20I TGGCCA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 278
MseI TTAA 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 278
MwoI GCNNNNNNNGC 4 cut(s) 137, 224, 275, 326
NdeII GATC 2 cut(s) 110, 243
NlaIII CATG 4 cut(s) 250, 269, 277, 341
NspI RCATGY 1 cut(s) 341
PaeI GCATGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 2 cut(s) 92, 328
Psp124BI GAGCTC 1 cut(s) 142
RsaI GTAC 2 cut(s) 73, 357
RsaNI GTAC 2 cut(s) 72, 356
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 2 cut(s) 91, 327
Sau3AI GATC 2 cut(s) 110, 243
SduI GDGCHC 2 cut(s) 142, 265
SetI ASST 5 cut(s) 33, 142, 149, 190, 301
SfaNI GCATC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SphI GCATGC 1 cut(s) 341
Sse9I AATT 4 cut(s) 59, 78, 173, 219
SspMI CTAG 2 cut(s) 32, 54
SstI GAGCTC 1 cut(s) 142
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 71
TasI AATT 4 cut(s) 59, 78, 173, 219
TatI WGTACW 2 cut(s) 71, 355
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseI GCWGC 2 cut(s) 90, 326
XapI RAATTY 1 cut(s) 78
XceI RCATGY 1 cut(s) 341
XspI CTAG 2 cut(s) 32, 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.