Rh2BG465300

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000605
Physical Location & Seq
Reverse (-)
65718556 .. 65723473
4918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG465300.1

Sequence Viewer

Length: 366 bp
ATGGCATCTGTTATTAGCTTTACTGCCGTGTCAATGGAGAACTATCATGATGGGATTAATGAAGGAAACGATCTAGGTTCAATAGATTTTGATATTCTGGTCCCAAATCTTGAGGTTATAGTTGGTGCTCGTGCTGTCATGGCCAATGGTGGTGTTATAGCACCTGTTGGTTTGAATATGGTTGCACTTGCAGCCCAAAGGCATGCTGTCCCTTTTGATGTGCTTGCTGGCGGTTACAAGGTTGCAACTTTGGCACCAAATGTCCCGGCTATGCATGAGCTTCATTTTGCAGCTCCAATGGCTAGAGCAGTTCTTTGTACGCTAAATTCACGCCTTAGATTCAGCTATGATATCTGTTCTTCTTAG

Protein Analysis

121

Amino Acids

12.74

Weight (kDa)

5.34

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 39 - 86 5.1e-10 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AciI CCGC 1 cut(s) 231
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 1 cut(s) 325
AfaI GTAC 1 cut(s) 319
AgsI TTSAA 2 cut(s) 81, 175
AluBI AGCT 4 cut(s) 18, 280, 293, 345
AluI AGCT 4 cut(s) 18, 280, 293, 345
Alw21I GWGCWC 1 cut(s) 130
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 191, 290
ApoI RAATTY 1 cut(s) 325
AseI ATTAAT 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 100
BalI TGGCCA 1 cut(s) 143
BanI GGYRCC 1 cut(s) 253
BauI CACGAG 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 203, 302
BccI CCATC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 11
BcnI CCSGG 1 cut(s) 266
BfaI CTAG 2 cut(s) 74, 303
BisI GCNGC 2 cut(s) 192, 291
BlsI GCNGC 2 cut(s) 193, 292
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 102, 255
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 1 cut(s) 14
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 266
BseXI GCAGC 2 cut(s) 203, 302
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 3 cut(s) 86, 194, 248
BsmFI GGGAC 3 cut(s) 86, 194, 248
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 1 cut(s) 70
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 143
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 102, 255
BspT107I GGYRCC 1 cut(s) 253
BssMI GATC 1 cut(s) 70
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 204, 225, 229
BstDEI CTNAG 2 cut(s) 335, 363
BstKTI GATC 1 cut(s) 73
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 4 cut(s) 140, 191, 251, 299
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 203, 302
BsuRI GGCC 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 204, 225, 229
CciI TCATGA 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 318
CviAII CATG 4 cut(s) 47, 139, 203, 275
CviJI RGCY 8 cut(s) 18, 143, 194, 269, 280, 293, 302, 345
CviKI_1 RGCY 8 cut(s) 18, 143, 194, 269, 280, 293, 302, 345
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 2 cut(s) 335, 363
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 141
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 100
EcoRV GATATC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 276
FaeI CATG 4 cut(s) 50, 142, 206, 278
FaiI YATR 9 cut(s) 48, 119, 140, 158, 179, 204, 272, 276, 348
FaqI GGGAC 3 cut(s) 86, 194, 248
FatI CATG 4 cut(s) 46, 138, 202, 274
Fnu4HI GCNGC 2 cut(s) 192, 291
Fsp4HI GCNGC 2 cut(s) 192, 291
FspBI CTAG 2 cut(s) 74, 303
GluI GCNGC 2 cut(s) 192, 291
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 266
Hin1II CATG 4 cut(s) 50, 142, 206, 278
HinfI GANTC 1 cut(s) 339
HpaII CCGG 1 cut(s) 266
Hpy188III TCNNGA 2 cut(s) 47, 110
HpyAV CCTTC 1 cut(s) 56
HpyCH4V TGCA 5 cut(s) 185, 191, 245, 274, 290
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 191, 251, 299
HpyF3I CTNAG 2 cut(s) 335, 363
Hsp92II CATG 4 cut(s) 50, 142, 206, 278
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 4 cut(s) 83, 177, 213, 279
Lsp1109I GCAGC 2 cut(s) 203, 302
LweI GCATC 1 cut(s) 14
MaeI CTAG 2 cut(s) 74, 303
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 143
MluCI AATT 1 cut(s) 325
MluNI TGGCCA 1 cut(s) 143
MnlI CCTC 1 cut(s) 106
Mox20I TGGCCA 1 cut(s) 143
Mph1103I ATGCAT 1 cut(s) 276
MscI TGGCCA 1 cut(s) 143
MseI TTAA 1 cut(s) 57
Msp20I TGGCCA 1 cut(s) 143
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 266
MwoI GCNNNNNNNGC 4 cut(s) 140, 191, 251, 299
NciI CCSGG 1 cut(s) 266
NdeII GATC 1 cut(s) 70
NlaIII CATG 4 cut(s) 50, 142, 206, 278
NlaIV GGNNCC 2 cut(s) 102, 255
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 206
PaeI GCATGC 1 cut(s) 206
PagI TCATGA 1 cut(s) 46
PfeI GAWTC 1 cut(s) 339
PkrI GCNGC 2 cut(s) 193, 292
PshBI ATTAAT 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 102, 255
PspPI GGNCC 1 cut(s) 100
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 57
SatI GCNGC 2 cut(s) 192, 291
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 1 cut(s) 130
SetI ASST 8 cut(s) 20, 79, 117, 166, 243, 282, 295, 347
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SphI GCATGC 1 cut(s) 206
Sse9I AATT 1 cut(s) 325
SsiI CCGC 1 cut(s) 231
SspMI CTAG 2 cut(s) 74, 303
StyD4I CCNGG 1 cut(s) 264
TasI AATT 1 cut(s) 325
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 1 cut(s) 57
Tru9I TTAA 1 cut(s) 57
TseI GCWGC 2 cut(s) 191, 290
TspDTI ATGAA 2 cut(s) 75, 272
VpaK11BI GGWCC 1 cut(s) 100
VspI ATTAAT 1 cut(s) 57
XapI RAATTY 1 cut(s) 325
XceI RCATGY 1 cut(s) 206
XspI CTAG 2 cut(s) 74, 303
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.