Rw4G004270

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000605
Physical Location & Seq
Forward (+)
8532066 .. 8535291
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004270.1

Sequence Viewer

Length: 1182 bp
ATGGCGGAAGTTCAAGCTCTCGTCAATGAACTCGCCACCAAGCTCCGGAAACGGAAGGTGGAGGGTTCACGAGAGACTGCTCGGCTGACGGCGGAGTTGTTACGGACGGTAATATCCCAGCAGAGGGTGACTTCAGCCGCCCTTGTTGAGGTCGTCAGAGATGTTGGTCAGCAGCTTATTGCTGCTAACCCTGTTGAGCTTGCTGTTGGGAACATTGTGAGGCGCGTGCTGCGCATCATTAGGGATGGGGATCTTTCACCGGAAGTTGGAGGATTGACTTTAGTCTCCCAGGGTGATGATGGAGATATTGTTGAGGAAGATGATAAACCATTTCTATTATCTGATGCTATTTCCCAAACAATTCTCCATCCACCTTCCTTGGTTGCACAGCTTGAAAGGAAACCCAACTTGGTTTGCCACTTTGAGGGAAAAGCAGAAAAAACTCCGAGTAGCTGGCAATTTAAACACCAGATCATTGAGGGAGTTAATGATCTCATTGAGGATATAAATACTTGCCATGAACTGATCGCAGACCAGTCGTTGGAGCTTATACATCAAAATGAGGTGATACTAACCTTAGGTCACTCGAGGACTGTCAAGAAGTTCCTCTATGCTGCAAAGGAGAAGAAAAGATCTTTCCAGGTCTATGTGGCCGAGGGTGCTCCTAAGCATTTGGGGCATGTGCTTGCAAACGAGTTAACTGAAAAGCATTTGCAAACAACCGTCATCCCTGACTCTTCAGTGTTTGCCATGATTTCAAGAGTTAACATGGTCATAGTCGGGGTTCATGCTGTGATGGCCAATGGTGGGGTCATAGCCCCTGTTGGGATGAATATGGTTGCACTTGCTGCAAAAAGGCATGCTGTTCCATTTGTTGTGGTCGGTGGCACTCATAAGTTATGTCCACTGTTTCCTAACAATCCAGAGGTTTTGCTGAACGATATGAGATGTCCATCTGAGCTATTATCTTTTGGGGACTTCTCAGATTGCATGGATTTTAGTGTTGGCAGTGGAGCTCCTCTTTTGCATGTTGTTAATCCTGCATTTGACTATGTGCCACCCGAGCTAGTCAGTCTTTTTGTTACTGAAACAGGGGGCTATCATCCATCATACATCTATAGGCTCATTTCTGATTATTACTCTGCTGAGGATATAGTTCTGCAACAAAAACCTGCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IF-2B

Protein Analysis

393

Amino Acids

43.09

Weight (kDa)

5.7

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 19 - 369 9.4e-62 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 541
AccII CGCG 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 3 cut(s) 5, 92, 138
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 2 cut(s) 651, 798
AcuI CTGAAG 2 cut(s) 117, 723
AfiI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 541, 807, 825
AgsI TTSAA 3 cut(s) 14, 395, 759
AjnI CCWGG 2 cut(s) 288, 639
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
Alw21I GWGCWC 2 cut(s) 664, 1018
Alw26I GTCTC 2 cut(s) 68, 289
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 2 cut(s) 586, 1061
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 2 cut(s) 651, 798
ApeKI GCWGC 5 cut(s) 172, 182, 229, 614, 848
AspLEI GCGC 2 cut(s) 225, 234
AsuHPI GGTGA 4 cut(s) 139, 249, 305, 577
AvaI CYCGRG 2 cut(s) 586, 1061
AxyI CCTNAGG 1 cut(s) 577
BalI TGGCCA 1 cut(s) 800
BanII GRGCYC 1 cut(s) 1018
BauI CACGAG 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 664, 1018
BbvCI CCTCAGC 1 cut(s) 1146
BbvI GCAGC 5 cut(s) 169, 184, 216, 601, 835
BccI CCATC 6 cut(s) 239, 293, 375, 790, 961, 1114
BceAI ACGGC 1 cut(s) 105
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciT130I CCWGG 2 cut(s) 290, 641
BcoDI GTCTC 2 cut(s) 68, 289
BfaI CTAG 1 cut(s) 1067
BfmI CTRYAG 1 cut(s) 1117
BglII AGATCT 1 cut(s) 632
BisI GCNGC 6 cut(s) 138, 173, 183, 230, 615, 849
BlsI GCNGC 6 cut(s) 139, 174, 184, 231, 616, 850
Bme1390I CCNGG 2 cut(s) 290, 641
BmeT110I CYCGRG 2 cut(s) 586, 1061
BmrFI CCNGG 2 cut(s) 290, 641
BmsI GCATC 2 cut(s) 243, 334
BoxI GACNNNNGTC 1 cut(s) 281
Bpu10I CCTNAGC 2 cut(s) 666, 1146
BsaBI GATNNNNATC 2 cut(s) 249, 952
BsaJI CCNNGG 4 cut(s) 288, 289, 378, 654
BsaWI WCCGGW 2 cut(s) 45, 259
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 541, 807, 825
Bse1I ACTGG 1 cut(s) 535
Bse21I CCTNAGG 1 cut(s) 577
Bse8I GATNNNNATC 2 cut(s) 249, 952
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 2 cut(s) 290, 641
BseDI CCNNGG 4 cut(s) 288, 289, 378, 654
BseGI GGATG 5 cut(s) 250, 367, 726, 834, 1102
BseJI GATNNNNATC 2 cut(s) 249, 952
BseLI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 541, 807, 825
BseMII CTCAG 3 cut(s) 948, 996, 1137
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 1 cut(s) 1008
BseXI GCAGC 5 cut(s) 169, 184, 216, 601, 835
BseYI CCCAGC 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 2 cut(s) 653, 800
BsiHKAI GWGCWC 2 cut(s) 664, 1018
BsiHKCI CYCGRG 2 cut(s) 586, 1061
BsiSI CCGG 2 cut(s) 46, 260
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 541, 807, 825
BsmAI GTCTC 2 cut(s) 68, 289
BsmFI GGGAC 1 cut(s) 989
BsnI GGCC 2 cut(s) 653, 800
BsoBI CYCGRG 2 cut(s) 586, 1061
Bsp1286I GDGCHC 2 cut(s) 664, 1018
Bsp13I TCCGGA 1 cut(s) 45
Bsp143I GATC 5 cut(s) 250, 471, 490, 525, 632
BspACI CCGC 3 cut(s) 5, 92, 138
BspANI GGCC 2 cut(s) 653, 800
BspCNI CTCAG 3 cut(s) 949, 995, 1138
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 1 cut(s) 225
BspPI GGATC 1 cut(s) 258
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 4 cut(s) 288, 289, 378, 654
BssMI GATC 5 cut(s) 250, 471, 490, 525, 632
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 290, 641
Bst4CI ACNGT 4 cut(s) 109, 595, 724, 909
Bst6I CTCTTC 1 cut(s) 742
BstAPI GCANNNNNTGC 1 cut(s) 848
BstC8I GCNNGC 5 cut(s) 201, 227, 455, 687, 861
BstDEI CTNAG 5 cut(s) 577, 666, 957, 982, 1146
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 5 cut(s) 250, 367, 726, 834, 1102
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 2 cut(s) 225, 234
BstKTI GATC 5 cut(s) 253, 474, 493, 528, 635
BstMAI GTCTC 2 cut(s) 68, 289
BstMBI GATC 5 cut(s) 250, 471, 490, 525, 632
BstMWI GCNNNNNNNGC 7 cut(s) 229, 231, 659, 676, 797, 848, 1063
BstNI CCWGG 2 cut(s) 290, 641
BstNSI RCATGY 3 cut(s) 683, 863, 1031
BstPAI GACNNNNGTC 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 288, 639
BstSFI CTRYAG 1 cut(s) 1117
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 5 cut(s) 169, 184, 216, 601, 835
BstX2I RGATCY 2 cut(s) 250, 632
BstYI RGATCY 2 cut(s) 250, 632
Bsu36I CCTNAGG 1 cut(s) 577
BsuRI GGCC 2 cut(s) 653, 800
BtsCI GGATG 5 cut(s) 250, 367, 726, 834, 1102
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 3 cut(s) 747, 905, 1015
Cac8I GCNNGC 5 cut(s) 201, 227, 455, 687, 861
CfoI GCGC 2 cut(s) 225, 234
CspCI CAANNNNNGTGG 2 cut(s) 360, 395
CviAII CATG 8 cut(s) 518, 680, 751, 769, 788, 860, 991, 1028
DdeI CTNAG 5 cut(s) 577, 666, 957, 982, 1146
DpnI GATC 5 cut(s) 252, 473, 492, 527, 634
DpnII GATC 5 cut(s) 250, 471, 490, 525, 632
DraI TTTAAA 1 cut(s) 463
EaeI YGGCCR 2 cut(s) 651, 798
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
EciI GGCGGA 2 cut(s) 20, 107
Ecl136II GAGCTC 1 cut(s) 1016
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 1018
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 2 cut(s) 117, 723
Eco81I CCTNAGG 1 cut(s) 577
Eco88I CYCGRG 2 cut(s) 586, 1061
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 288, 639
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 1018
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 8 cut(s) 521, 683, 754, 772, 791, 863, 994, 1031
FaqI GGGAC 1 cut(s) 989
FatI CATG 8 cut(s) 517, 679, 750, 768, 787, 859, 990, 1027
Fnu4HI GCNGC 6 cut(s) 138, 173, 183, 230, 615, 849
FokI GGATG 5 cut(s) 257, 354, 713, 841, 1089
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 6 cut(s) 138, 173, 183, 230, 615, 849
FspBI CTAG 1 cut(s) 1067
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 2 cut(s) 224, 233
GluI GCNGC 6 cut(s) 138, 173, 183, 230, 615, 849
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 653, 800
HapII CCGG 2 cut(s) 46, 260
HhaI GCGC 2 cut(s) 225, 234
Hin1II CATG 8 cut(s) 521, 683, 754, 772, 791, 863, 994, 1031
Hin6I GCGC 2 cut(s) 223, 232
HinP1I GCGC 2 cut(s) 223, 232
HincII GTYRAC 2 cut(s) 699, 766
HindII GTYRAC 2 cut(s) 699, 766
HinfI GANTC 1 cut(s) 734
HpaI GTTAAC 2 cut(s) 699, 766
HpaII CCGG 2 cut(s) 46, 260
HphI GGTGA 4 cut(s) 139, 249, 305, 577
Hpy166II GTNNAC 4 cut(s) 68, 699, 766, 905
Hpy188I TCNGA 6 cut(s) 158, 343, 447, 958, 985, 1132
Hpy188III TCNNGA 6 cut(s) 46, 69, 598, 759, 923, 1179
Hpy8I GTNNAC 4 cut(s) 68, 699, 766, 905
HpyAV CCTTC 2 cut(s) 49, 384
HpyCH4III ACNGT 4 cut(s) 109, 595, 724, 909
HpyF10VI GCNNNNNNNGC 7 cut(s) 229, 231, 659, 676, 797, 848, 1063
HpyF3I CTNAG 5 cut(s) 577, 666, 957, 982, 1146
Hsp92II CATG 8 cut(s) 521, 683, 754, 772, 791, 863, 994, 1031
HspAI GCGC 2 cut(s) 223, 232
Kpn2I TCCGGA 1 cut(s) 45
KspAI GTTAAC 2 cut(s) 699, 766
Kzo9I GATC 5 cut(s) 250, 471, 490, 525, 632
LmnI GCTCC 5 cut(s) 48, 544, 667, 1013, 1021
Lsp1109I GCAGC 5 cut(s) 169, 184, 216, 601, 835
LweI GCATC 2 cut(s) 243, 334
MaeI CTAG 1 cut(s) 1067
MaeIII GTNAC 4 cut(s) 99, 127, 581, 1081
MalI GATC 5 cut(s) 252, 473, 492, 527, 634
MboI GATC 5 cut(s) 250, 471, 490, 525, 632
MboII GAAGA 3 cut(s) 329, 637, 729
MflI RGATCY 2 cut(s) 250, 632
MhlI GDGCHC 2 cut(s) 664, 1018
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 2 cut(s) 360, 458
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 2 cut(s) 247, 522
Mox20I TGGCCA 1 cut(s) 800
MroI TCCGGA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 800
MseI TTAA 5 cut(s) 462, 486, 698, 765, 1035
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 800
MspI CCGG 2 cut(s) 46, 260
MspR9I CCNGG 2 cut(s) 290, 641
MvaI CCWGG 2 cut(s) 290, 641
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 7 cut(s) 229, 231, 659, 676, 797, 848, 1063
NdeII GATC 5 cut(s) 250, 471, 490, 525, 632
NlaIII CATG 8 cut(s) 521, 683, 754, 772, 791, 863, 994, 1031
NmeAIII GCCGAG 2 cut(s) 61, 679
NmuCI GTSAC 2 cut(s) 127, 581
NsbI TGCGCA 1 cut(s) 233
NspI RCATGY 3 cut(s) 683, 863, 1031
PaeI GCATGC 1 cut(s) 863
PaeR7I CTCGAG 1 cut(s) 586
PasI CCCWGGG 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 6 cut(s) 139, 174, 184, 231, 616, 850
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
PshAI GACNNNNGTC 1 cut(s) 281
Psp124BI GAGCTC 1 cut(s) 1018
Psp6I CCWGG 2 cut(s) 288, 639
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 288, 639
PspXI VCTCGAGB 1 cut(s) 586
PsuI RGATCY 2 cut(s) 250, 632
RseI CAYNNNNRTG 1 cut(s) 558
SacI GAGCTC 1 cut(s) 1018
SaqAI TTAA 5 cut(s) 462, 486, 698, 765, 1035
SatI GCNGC 6 cut(s) 138, 173, 183, 230, 615, 849
Sau3AI GATC 5 cut(s) 250, 471, 490, 525, 632
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 2 cut(s) 290, 641
SduI GDGCHC 2 cut(s) 664, 1018
SfaNI GCATC 2 cut(s) 243, 334
SfcI CTRYAG 1 cut(s) 1117
Sfr274I CTCGAG 1 cut(s) 586
SlaI CTCGAG 1 cut(s) 586
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SphI GCATGC 1 cut(s) 863
Sse9I AATT 2 cut(s) 360, 458
SsiI CCGC 3 cut(s) 5, 92, 138
SspMI CTAG 1 cut(s) 1067
SstI GAGCTC 1 cut(s) 1018
StyD4I CCNGG 2 cut(s) 288, 639
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 109, 595, 724, 909
TaqI TCGA 1 cut(s) 587
TasI AATT 2 cut(s) 360, 458
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 5 cut(s) 462, 486, 698, 765, 1035
Tru9I TTAA 5 cut(s) 462, 486, 698, 765, 1035
TscAI CASTG 3 cut(s) 747, 912, 1015
TseFI GTSAC 2 cut(s) 127, 581
TseI GCWGC 5 cut(s) 172, 182, 229, 614, 848
Tsp45I GTSAC 2 cut(s) 127, 581
TspDTI ATGAA 4 cut(s) 42, 534, 776, 845
TspGWI ACGGA 2 cut(s) 67, 118
TspRI CASTG 3 cut(s) 747, 912, 1015
Van91I CCANNNNNTGG 1 cut(s) 541
XagI CCTNNNNNAGG 1 cut(s) 146
XceI RCATGY 3 cut(s) 683, 863, 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 586
XspI CTAG 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.