RchiOBHm_Chr4g0394571

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000605
Physical Location & Seq
Forward (+)
10225092 .. 10229097
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36706

Sequence Viewer

Length: 1185 bp
ATGGCGGAAGTTCAAGCTCTCGTCAATGAACTCGCCACCAAGCTCCGGAAACGGAAGGTGGAGGGTTCACGAGAGACTGCTCGGCTGACGGCGGAGTTGTTACGGACGGTAATATCCCAGCAGAGGGTGACTTCAGCCGCCCTTGTTGAGGTCGTCAGAGATGTTGGTCAGCAGCTTATTGCTGCTAACCCTGTTGAGCTTGCTGTTGGGAACATTGTGAGGCGTGTTCTGCGCATCATTAGGGATGGGGATCTTTCACCGGAAGTTGGAGGATTGACTTTAGTCTCCCAGGGTGATGATGGAGATATTGTTGAGGAAGATGATAAACCATTTCTATTATCTGATGCTATTTCCCAAACAATTCTCCATCCACCTTCCTTGGTTGCACAGCTTGAAAGCAAACCCAACTTGGTTTGCCACTTTGAGGGAAAAGCAGCAGATAAAACTCCGAGTAGCTGGCAATTTAAACACCAGATCATTGAGGGAGTTAATGATCTCATTGAGGATATAAATACTTGCCATGAACTGATCGCAGACCAGTCGTTGGAGCTTATACATCAAAATGAGGTGATACTAACCTTAGGTCACTCGAGGACTGTCAAGAAGTTCCTCTATGCTGCAAAGGAGAAGAAAAGATCTTTCCAGGTCTATGTAGCCGAGGGTGCTCCTAAGCATTTGGGGCATGTGCTTGCAAACGAGTTAACTGAAAAGCATTTGCAAACAACCGTCATCCCTGACTCTTCAGTGTTTGCCATGATTTCAAGAGTTAACATGGTCATAGTCGGGGTTCATGCTGTGATGGCCAATGGTGGGGTCATAGCCCCTGTTGGGATGAATATGGTTGCACTTGCTGCAAAAAGGCATGCTGTTCCATTTGTTGTGGTTGGTGGCACTCATAAGTTATGTCCACTGTTTCCTAACAATCCAGAGGTTTTGCTGAATGATATGAGATGTCCATCTGAGCTATTATCTTTTGGGGACTTCTCAGATTGCATGGATTTTAGTGTTGGCAGTGGAGCTGCTCTTTTGCATGTTGTTAATCCTGCATTTGATTATGTGCCACCCGAGCTAGTCAGTCTTTTTGTTACTGAAATAGGGGGTTATCATCCATCATACATCTACAGGCTCATTTCGGATTATTACTCTGCTGACGATATAGTTCTGCAACAAAAACCTGCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IF-2B

Protein Analysis

394

Amino Acids

43.05

Weight (kDa)

5.61

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 19 - 370 5.2e-62 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 544
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 3 cut(s) 5, 92, 138
AclWI GGATC 1 cut(s) 258
AcoI YGGCCR 1 cut(s) 801
AcuI CTGAAG 2 cut(s) 117, 726
AfiI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 544, 810, 828
AgsI TTSAA 3 cut(s) 14, 395, 762
AjnI CCWGG 2 cut(s) 288, 642
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 2 cut(s) 68, 289
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 2 cut(s) 589, 1064
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 1 cut(s) 801
ApeKI GCWGC 6 cut(s) 172, 182, 434, 617, 851, 1019
AspLEI GCGC 1 cut(s) 234
AsuHPI GGTGA 4 cut(s) 139, 249, 305, 580
AvaI CYCGRG 2 cut(s) 589, 1064
AxyI CCTNAGG 1 cut(s) 580
BalI TGGCCA 1 cut(s) 803
BauI CACGAG 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 6 cut(s) 169, 184, 446, 604, 838, 1006
BccI CCATC 6 cut(s) 239, 293, 375, 793, 964, 1117
BceAI ACGGC 1 cut(s) 105
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BciT130I CCWGG 2 cut(s) 290, 644
BcoDI GTCTC 2 cut(s) 68, 289
BfaI CTAG 1 cut(s) 1070
BfmI CTRYAG 1 cut(s) 1120
BglII AGATCT 1 cut(s) 635
BisI GCNGC 7 cut(s) 138, 173, 183, 435, 618, 852, 1020
BlsI GCNGC 7 cut(s) 139, 174, 184, 436, 619, 853, 1021
Bme1390I CCNGG 2 cut(s) 290, 644
BmeT110I CYCGRG 2 cut(s) 589, 1064
BmrFI CCNGG 2 cut(s) 290, 644
BmsI GCATC 2 cut(s) 243, 334
BoxI GACNNNNGTC 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 669
BsaBI GATNNNNATC 2 cut(s) 249, 955
BsaJI CCNNGG 4 cut(s) 288, 289, 378, 657
BsaWI WCCGGW 2 cut(s) 45, 259
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 544, 810, 828
Bse1I ACTGG 1 cut(s) 538
Bse21I CCTNAGG 1 cut(s) 580
Bse8I GATNNNNATC 2 cut(s) 249, 955
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 2 cut(s) 290, 644
BseDI CCNNGG 4 cut(s) 288, 289, 378, 657
BseGI GGATG 5 cut(s) 250, 367, 729, 837, 1105
BseJI GATNNNNATC 2 cut(s) 249, 955
BseLI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 544, 810, 828
BseMII CTCAG 2 cut(s) 951, 999
BseNI ACTGG 1 cut(s) 538
BseXI GCAGC 6 cut(s) 169, 184, 446, 604, 838, 1006
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 1 cut(s) 803
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 2 cut(s) 589, 1064
BsiSI CCGG 2 cut(s) 46, 260
BslFI GGGAC 1 cut(s) 992
BslI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 544, 810, 828
BsmAI GTCTC 2 cut(s) 68, 289
BsmFI GGGAC 1 cut(s) 992
BsnI GGCC 1 cut(s) 803
BsoBI CYCGRG 2 cut(s) 589, 1064
Bsp1286I GDGCHC 1 cut(s) 667
Bsp13I TCCGGA 1 cut(s) 45
Bsp143I GATC 5 cut(s) 250, 474, 493, 528, 635
BspACI CCGC 3 cut(s) 5, 92, 138
BspANI GGCC 1 cut(s) 803
BspCNI CTCAG 2 cut(s) 952, 998
BspEI TCCGGA 1 cut(s) 45
BspPI GGATC 1 cut(s) 258
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 4 cut(s) 288, 289, 378, 657
BssMI GATC 5 cut(s) 250, 474, 493, 528, 635
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 290, 644
Bst4CI ACNGT 4 cut(s) 109, 598, 727, 912
Bst6I CTCTTC 1 cut(s) 745
BstAPI GCANNNNNTGC 1 cut(s) 851
BstC8I GCNNGC 4 cut(s) 201, 458, 690, 864
BstDEI CTNAG 4 cut(s) 580, 669, 960, 985
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 5 cut(s) 250, 367, 729, 837, 1105
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 5 cut(s) 253, 477, 496, 531, 638
BstMAI GTCTC 2 cut(s) 68, 289
BstMBI GATC 5 cut(s) 250, 474, 493, 528, 635
BstMWI GCNNNNNNNGC 6 cut(s) 229, 662, 679, 800, 851, 1066
BstNI CCWGG 2 cut(s) 290, 644
BstNSI RCATGY 3 cut(s) 686, 866, 1034
BstPAI GACNNNNGTC 1 cut(s) 281
BstSCI CCNGG 2 cut(s) 288, 642
BstSFI CTRYAG 1 cut(s) 1120
BstV1I GCAGC 6 cut(s) 169, 184, 446, 604, 838, 1006
BstX2I RGATCY 2 cut(s) 250, 635
BstYI RGATCY 2 cut(s) 250, 635
Bsu36I CCTNAGG 1 cut(s) 580
BsuRI GGCC 1 cut(s) 803
BtsCI GGATG 5 cut(s) 250, 367, 729, 837, 1105
BtsI GCAGTG 1 cut(s) 1018
BtsIMutI CAGTG 3 cut(s) 750, 908, 1018
Cac8I GCNNGC 4 cut(s) 201, 458, 690, 864
CfoI GCGC 1 cut(s) 234
CspCI CAANNNNNGTGG 2 cut(s) 360, 395
CviAII CATG 8 cut(s) 521, 683, 754, 772, 791, 863, 994, 1031
DdeI CTNAG 4 cut(s) 580, 669, 960, 985
DpnI GATC 5 cut(s) 252, 476, 495, 530, 637
DpnII GATC 5 cut(s) 250, 474, 493, 528, 635
DraI TTTAAA 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 801
Eam1104I CTCTTC 1 cut(s) 745
EarI CTCTTC 1 cut(s) 745
EciI GGCGGA 2 cut(s) 20, 107
Eco130I CCWWGG 1 cut(s) 378
Eco57I CTGAAG 2 cut(s) 117, 726
Eco81I CCTNAGG 1 cut(s) 580
Eco88I CYCGRG 2 cut(s) 589, 1064
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 288, 642
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 8 cut(s) 524, 686, 757, 775, 794, 866, 997, 1034
FaqI GGGAC 1 cut(s) 992
FatI CATG 8 cut(s) 520, 682, 753, 771, 790, 862, 993, 1030
Fnu4HI GCNGC 7 cut(s) 138, 173, 183, 435, 618, 852, 1020
FokI GGATG 5 cut(s) 257, 354, 716, 844, 1092
Fsp4HI GCNGC 7 cut(s) 138, 173, 183, 435, 618, 852, 1020
FspBI CTAG 1 cut(s) 1070
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 1 cut(s) 233
GluI GCNGC 7 cut(s) 138, 173, 183, 435, 618, 852, 1020
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 1 cut(s) 803
HapII CCGG 2 cut(s) 46, 260
HhaI GCGC 1 cut(s) 234
Hin1II CATG 8 cut(s) 524, 686, 757, 775, 794, 866, 997, 1034
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 2 cut(s) 702, 769
HindII GTYRAC 2 cut(s) 702, 769
HinfI GANTC 1 cut(s) 737
HpaI GTTAAC 2 cut(s) 702, 769
HpaII CCGG 2 cut(s) 46, 260
HphI GGTGA 4 cut(s) 139, 249, 305, 580
Hpy166II GTNNAC 4 cut(s) 68, 702, 769, 908
Hpy188I TCNGA 6 cut(s) 158, 343, 450, 961, 988, 1135
Hpy188III TCNNGA 6 cut(s) 46, 69, 601, 762, 926, 1182
Hpy8I GTNNAC 4 cut(s) 68, 702, 769, 908
HpyAV CCTTC 2 cut(s) 49, 384
HpyCH4III ACNGT 4 cut(s) 109, 598, 727, 912
HpyF10VI GCNNNNNNNGC 6 cut(s) 229, 662, 679, 800, 851, 1066
HpyF3I CTNAG 4 cut(s) 580, 669, 960, 985
Hsp92II CATG 8 cut(s) 524, 686, 757, 775, 794, 866, 997, 1034
HspAI GCGC 1 cut(s) 232
Kpn2I TCCGGA 1 cut(s) 45
KspAI GTTAAC 2 cut(s) 702, 769
Kzo9I GATC 5 cut(s) 250, 474, 493, 528, 635
LmnI GCTCC 4 cut(s) 48, 547, 670, 1016
Lsp1109I GCAGC 6 cut(s) 169, 184, 446, 604, 838, 1006
LweI GCATC 2 cut(s) 243, 334
MaeI CTAG 1 cut(s) 1070
MaeIII GTNAC 4 cut(s) 99, 127, 584, 1084
MalI GATC 5 cut(s) 252, 476, 495, 530, 637
MboI GATC 5 cut(s) 250, 474, 493, 528, 635
MboII GAAGA 3 cut(s) 329, 640, 732
MflI RGATCY 2 cut(s) 250, 635
MhlI GDGCHC 1 cut(s) 667
MlsI TGGCCA 1 cut(s) 803
MluCI AATT 2 cut(s) 360, 461
MluNI TGGCCA 1 cut(s) 803
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 2 cut(s) 247, 525
Mox20I TGGCCA 1 cut(s) 803
MroI TCCGGA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 803
MseI TTAA 5 cut(s) 465, 489, 701, 768, 1038
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 803
MspI CCGG 2 cut(s) 46, 260
MspR9I CCNGG 2 cut(s) 290, 644
MvaI CCWGG 2 cut(s) 290, 644
MwoI GCNNNNNNNGC 6 cut(s) 229, 662, 679, 800, 851, 1066
NdeII GATC 5 cut(s) 250, 474, 493, 528, 635
NlaIII CATG 8 cut(s) 524, 686, 757, 775, 794, 866, 997, 1034
NmeAIII GCCGAG 2 cut(s) 61, 682
NmuCI GTSAC 2 cut(s) 127, 584
NsbI TGCGCA 1 cut(s) 233
NspI RCATGY 3 cut(s) 686, 866, 1034
PaeI GCATGC 1 cut(s) 866
PaeR7I CTCGAG 1 cut(s) 589
PasI CCCWGGG 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 7 cut(s) 139, 174, 184, 436, 619, 853, 1021
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PshAI GACNNNNGTC 1 cut(s) 281
Psp6I CCWGG 2 cut(s) 288, 642
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 2 cut(s) 288, 642
PspXI VCTCGAGB 1 cut(s) 589
PsuI RGATCY 2 cut(s) 250, 635
RseI CAYNNNNRTG 1 cut(s) 561
SaqAI TTAA 5 cut(s) 465, 489, 701, 768, 1038
SatI GCNGC 7 cut(s) 138, 173, 183, 435, 618, 852, 1020
Sau3AI GATC 5 cut(s) 250, 474, 493, 528, 635
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 2 cut(s) 290, 644
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 2 cut(s) 243, 334
SfcI CTRYAG 1 cut(s) 1120
Sfr274I CTCGAG 1 cut(s) 589
SlaI CTCGAG 1 cut(s) 589
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 589
SmoI CTYRAG 1 cut(s) 589
SphI GCATGC 1 cut(s) 866
Sse9I AATT 2 cut(s) 360, 461
SsiI CCGC 3 cut(s) 5, 92, 138
SspMI CTAG 1 cut(s) 1070
StyD4I CCNGG 2 cut(s) 288, 642
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 4 cut(s) 109, 598, 727, 912
TaqI TCGA 1 cut(s) 590
TasI AATT 2 cut(s) 360, 461
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 5 cut(s) 465, 489, 701, 768, 1038
Tru9I TTAA 5 cut(s) 465, 489, 701, 768, 1038
TscAI CASTG 3 cut(s) 750, 915, 1018
TseFI GTSAC 2 cut(s) 127, 584
TseI GCWGC 6 cut(s) 172, 182, 434, 617, 851, 1019
Tsp45I GTSAC 2 cut(s) 127, 584
TspDTI ATGAA 4 cut(s) 42, 537, 779, 848
TspGWI ACGGA 2 cut(s) 67, 118
TspRI CASTG 3 cut(s) 750, 915, 1018
Van91I CCANNNNNTGG 1 cut(s) 544
XagI CCTNNNNNAGG 1 cut(s) 146
XceI RCATGY 3 cut(s) 686, 866, 1034
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XhoI CTCGAG 1 cut(s) 589
XspI CTAG 1 cut(s) 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.