Belongs to the eIF-2B alpha beta delta subunits family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1246 |
| AciI | CCGC | 2 cut(s) | 235, 401 |
| AclWI | GGATC | 1 cut(s) | 270 |
| AcoI | YGGCCR | 1 cut(s) | 864 |
| AcsI | RAATTY | 2 cut(s) | 668, 1043 |
| AcuI | CTGAAG | 3 cut(s) | 501, 548, 1011 |
| AfaI | GTAC | 3 cut(s) | 663, 945, 1184 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| AflIII | ACRYGT | 1 cut(s) | 1179 |
| AgsI | TTSAA | 6 cut(s) | 298, 425, 545, 566, 654, 898 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 929 |
| AjiI | CACGTC | 1 cut(s) | 10 |
| Alw21I | GWGCWC | 3 cut(s) | 615, 730, 853 |
| AlwI | GGATC | 1 cut(s) | 270 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 380 |
| Ama87I | CYCGRG | 1 cut(s) | 825 |
| AoxI | GGCC | 2 cut(s) | 864, 1162 |
| ApoI | RAATTY | 2 cut(s) | 668, 1043 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 29, 561 |
| AsuC2I | CCSGG | 1 cut(s) | 107 |
| AsuHPI | GGTGA | 4 cut(s) | 121, 265, 485, 611 |
| AsuII | TTCGAA | 1 cut(s) | 1066 |
| AvaI | CYCGRG | 1 cut(s) | 825 |
| BaeGI | GKGCMC | 2 cut(s) | 132, 971 |
| BalI | TGGCCA | 1 cut(s) | 866 |
| BanII | GRGCYC | 2 cut(s) | 68, 730 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 1225 |
| BbsI | GAAGAC | 2 cut(s) | 432, 1240 |
| Bbv12I | GWGCWC | 3 cut(s) | 615, 730, 853 |
| BccI | CCATC | 1 cut(s) | 1180 |
| BceAI | ACGGC | 2 cut(s) | 93, 914 |
| BciVI | GTATCC | 1 cut(s) | 987 |
| BclI | TGATCA | 1 cut(s) | 792 |
| BcnI | CCSGG | 1 cut(s) | 107 |
| BfaI | CTAG | 4 cut(s) | 513, 644, 665, 1031 |
| BfmI | CTRYAG | 2 cut(s) | 366, 1242 |
| BfuAI | ACCTGC | 1 cut(s) | 1246 |
| BfuI | GTATCC | 1 cut(s) | 987 |
| BglII | AGATCT | 1 cut(s) | 1013 |
| BlpI | GCTNAGC | 1 cut(s) | 597 |
| BmcAI | AGTACT | 1 cut(s) | 663 |
| Bme1390I | CCNGG | 1 cut(s) | 107 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 929 |
| BmeT110I | CYCGRG | 1 cut(s) | 825 |
| BmgBI | CACGTC | 1 cut(s) | 10 |
| BmiI | GGNNCC | 2 cut(s) | 151, 1081 |
| BmrFI | CCNGG | 1 cut(s) | 107 |
| BmsI | GCATC | 3 cut(s) | 151, 427, 754 |
| BpiI | GAAGAC | 2 cut(s) | 432, 1240 |
| BpmI | CTGGAG | 1 cut(s) | 1179 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 597 |
| Bpu14I | TTCGAA | 1 cut(s) | 1066 |
| BpuMI | CCSGG | 1 cut(s) | 107 |
| BsaJI | CCNNGG | 1 cut(s) | 731 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| Bse118I | RCCGGY | 1 cut(s) | 1185 |
| Bse1I | ACTGG | 2 cut(s) | 1081, 1162 |
| BseDI | CCNNGG | 1 cut(s) | 731 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| BseMII | CTCAG | 4 cut(s) | 84, 588, 1014, 1062 |
| BseNI | ACTGG | 2 cut(s) | 1081, 1162 |
| BseRI | GAGGAG | 1 cut(s) | 272 |
| BseSI | GKGCMC | 2 cut(s) | 132, 971 |
| BseYI | CCCAGC | 1 cut(s) | 290 |
| BshFI | GGCC | 2 cut(s) | 866, 1164 |
| BsiHKAI | GWGCWC | 3 cut(s) | 615, 730, 853 |
| BsiHKCI | CYCGRG | 1 cut(s) | 825 |
| BsiSI | CCGG | 4 cut(s) | 107, 147, 520, 1186 |
| BslFI | GGGAC | 1 cut(s) | 917 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| BsmFI | GGGAC | 1 cut(s) | 917 |
| BsnI | GGCC | 2 cut(s) | 866, 1164 |
| BsoBI | CYCGRG | 1 cut(s) | 825 |
| Bsp119I | TTCGAA | 1 cut(s) | 1066 |
| Bsp1286I | GDGCHC | 6 cut(s) | 68, 132, 615, 730, 853, 971 |
| Bsp143I | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 597 |
| BspACI | CCGC | 2 cut(s) | 235, 401 |
| BspANI | GGCC | 2 cut(s) | 866, 1164 |
| BspCNI | CTCAG | 4 cut(s) | 85, 589, 1015, 1061 |
| BspLI | GGNNCC | 2 cut(s) | 151, 1081 |
| BspMI | ACCTGC | 1 cut(s) | 1246 |
| BspPI | GGATC | 1 cut(s) | 270 |
| BspT104I | TTCGAA | 1 cut(s) | 1066 |
| BsrFI | RCCGGY | 1 cut(s) | 1185 |
| BsrI | ACTGG | 2 cut(s) | 1081, 1162 |
| BssAI | RCCGGY | 1 cut(s) | 1185 |
| BssECI | CCNNGG | 1 cut(s) | 731 |
| BssMI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| BssT1I | CCWWGG | 1 cut(s) | 731 |
| Bst4CI | ACNGT | 2 cut(s) | 310, 661 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 127, 380, 812 |
| BstBI | TTCGAA | 1 cut(s) | 1066 |
| BstC8I | GCNNGC | 5 cut(s) | 68, 72, 213, 603, 952 |
| BstDEI | CTNAG | 4 cut(s) | 93, 597, 1023, 1048 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| BstKTI | GATC | 4 cut(s) | 265, 701, 795, 1016 |
| BstMBI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| BstNSI | RCATGY | 4 cut(s) | 433, 929, 1097, 1183 |
| BstSCI | CCNGG | 1 cut(s) | 105 |
| BstSFI | CTRYAG | 2 cut(s) | 366, 1242 |
| BstSLI | GKGCMC | 2 cut(s) | 132, 971 |
| BstV2I | GAAGAC | 2 cut(s) | 432, 1240 |
| BstX2I | RGATCY | 2 cut(s) | 262, 1013 |
| BstYI | RGATCY | 2 cut(s) | 262, 1013 |
| BsuI | GTATCC | 1 cut(s) | 987 |
| BsuRI | GGCC | 2 cut(s) | 866, 1164 |
| BtgZI | GCGATG | 1 cut(s) | 1177 |
| BtrI | CACGTC | 1 cut(s) | 10 |
| BtsI | GCAGTG | 1 cut(s) | 392 |
| BtsIMutI | CAGTG | 5 cut(s) | 319, 392, 795, 1074, 1149 |
| BveI | ACCTGC | 1 cut(s) | 1246 |
| Cac8I | GCNNGC | 5 cut(s) | 68, 72, 213, 603, 952 |
| CaiI | CAGNNNCTG | 1 cut(s) | 380 |
| Cfr10I | RCCGGY | 1 cut(s) | 1185 |
| CseI | GACGC | 1 cut(s) | 386 |
| Csp6I | GTAC | 3 cut(s) | 662, 944, 1183 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 186, 221 |
| CviQI | GTAC | 3 cut(s) | 662, 944, 1183 |
| DdeI | CTNAG | 4 cut(s) | 93, 597, 1023, 1048 |
| DpnI | GATC | 4 cut(s) | 264, 700, 794, 1015 |
| DpnII | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| DriI | GACNNNNNGTC | 1 cut(s) | 929 |
| EaeI | YGGCCR | 1 cut(s) | 864 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 929 |
| EciI | GGCGGA | 1 cut(s) | 390 |
| Ecl136II | GAGCTC | 1 cut(s) | 728 |
| Eco130I | CCWWGG | 1 cut(s) | 731 |
| Eco24I | GRGCYC | 2 cut(s) | 68, 730 |
| Eco32I | GATATC | 1 cut(s) | 822 |
| Eco53kI | GAGCTC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 3 cut(s) | 501, 548, 1011 |
| Eco88I | CYCGRG | 1 cut(s) | 825 |
| EcoICRI | GAGCTC | 1 cut(s) | 728 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| EcoRI | GAATTC | 2 cut(s) | 668, 1043 |
| EcoRV | GATATC | 1 cut(s) | 822 |
| EcoT14I | CCWWGG | 1 cut(s) | 731 |
| EcoT38I | GRGCYC | 2 cut(s) | 68, 730 |
| ErhI | CCWWGG | 1 cut(s) | 731 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1003, 1035 |
| FaqI | GGGAC | 1 cut(s) | 917 |
| FauI | CCCGC | 1 cut(s) | 228 |
| FbaI | TGATCA | 1 cut(s) | 792 |
| FriOI | GRGCYC | 2 cut(s) | 68, 730 |
| FspBI | CTAG | 4 cut(s) | 513, 644, 665, 1031 |
| GsaI | CCCAGC | 1 cut(s) | 294 |
| GsuI | CTGGAG | 1 cut(s) | 1179 |
| HaeIII | GGCC | 2 cut(s) | 866, 1164 |
| HapII | CCGG | 4 cut(s) | 107, 147, 520, 1186 |
| HgaI | GACGC | 1 cut(s) | 386 |
| HincII | GTYRAC | 2 cut(s) | 306, 334 |
| HindII | GTYRAC | 2 cut(s) | 306, 334 |
| HindIII | AAGCTT | 1 cut(s) | 1130 |
| HinfI | GANTC | 3 cut(s) | 139, 476, 800 |
| HpaII | CCGG | 4 cut(s) | 107, 147, 520, 1186 |
| HphI | GGTGA | 4 cut(s) | 121, 265, 485, 611 |
| Hpy166II | GTNNAC | 4 cut(s) | 306, 334, 779, 832 |
| Hpy188I | TCNGA | 3 cut(s) | 481, 1024, 1051 |
| Hpy188III | TCNNGA | 4 cut(s) | 422, 513, 566, 825 |
| Hpy8I | GTNNAC | 4 cut(s) | 306, 334, 779, 832 |
| Hpy99I | CGWCG | 1 cut(s) | 121 |
| HpyAV | CCTTC | 4 cut(s) | 414, 428, 476, 1029 |
| HpyCH4III | ACNGT | 2 cut(s) | 310, 661 |
| HpyCH4IV | ACGT | 1 cut(s) | 9 |
| HpyF3I | CTNAG | 4 cut(s) | 93, 597, 1023, 1048 |
| HpySE526I | ACGT | 1 cut(s) | 9 |
| Ksp22I | TGATCA | 1 cut(s) | 792 |
| Kzo9I | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| LmnI | GCTCC | 6 cut(s) | 149, 181, 208, 725, 733, 1248 |
| LweI | GCATC | 3 cut(s) | 151, 427, 754 |
| MaeI | CTAG | 4 cut(s) | 513, 644, 665, 1031 |
| MaeII | ACGT | 1 cut(s) | 9 |
| MaeIII | GTNAC | 2 cut(s) | 96, 956 |
| MalI | GATC | 4 cut(s) | 264, 700, 794, 1015 |
| MboI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| MboII | GAAGA | 5 cut(s) | 335, 437, 453, 740, 1245 |
| MflI | RGATCY | 2 cut(s) | 262, 1013 |
| MhlI | GDGCHC | 6 cut(s) | 68, 132, 615, 730, 853, 971 |
| MlsI | TGGCCA | 1 cut(s) | 866 |
| MluCI | AATT | 4 cut(s) | 668, 761, 807, 1043 |
| MluNI | TGGCCA | 1 cut(s) | 866 |
| MmeI | TCCRAC | 1 cut(s) | 1252 |
| Mox20I | TGGCCA | 1 cut(s) | 866 |
| MroXI | GAANNNNTTC | 2 cut(s) | 29, 561 |
| MscI | TGGCCA | 1 cut(s) | 866 |
| MseI | TTAA | 2 cut(s) | 638, 1010 |
| MslI | CAYNNNNRTG | 1 cut(s) | 624 |
| Msp20I | TGGCCA | 1 cut(s) | 866 |
| MspA1I | CMGCKG | 3 cut(s) | 187, 281, 380 |
| MspI | CCGG | 4 cut(s) | 107, 147, 520, 1186 |
| MspR9I | CCNGG | 1 cut(s) | 107 |
| NciI | CCSGG | 1 cut(s) | 107 |
| NdeII | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| NlaIV | GGNNCC | 2 cut(s) | 151, 1081 |
| NmuCI | GTSAC | 1 cut(s) | 956 |
| NspI | RCATGY | 4 cut(s) | 433, 929, 1097, 1183 |
| NspV | TTCGAA | 1 cut(s) | 1066 |
| PaeR7I | CTCGAG | 1 cut(s) | 825 |
| PciI | ACATGT | 1 cut(s) | 1179 |
| PdmI | GAANNNNTTC | 2 cut(s) | 29, 561 |
| PfeI | GAWTC | 3 cut(s) | 139, 476, 800 |
| PscI | ACATGT | 1 cut(s) | 1179 |
| Psp124BI | GAGCTC | 1 cut(s) | 730 |
| PspFI | CCCAGC | 1 cut(s) | 290 |
| PspN4I | GGNNCC | 2 cut(s) | 151, 1081 |
| PstNI | CAGNNNCTG | 1 cut(s) | 380 |
| PsuI | RGATCY | 2 cut(s) | 262, 1013 |
| PvuII | CAGCTG | 3 cut(s) | 187, 281, 380 |
| RsaI | GTAC | 3 cut(s) | 663, 945, 1184 |
| RsaNI | GTAC | 3 cut(s) | 662, 944, 1183 |
| RseI | CAYNNNNRTG | 1 cut(s) | 624 |
| SacI | GAGCTC | 1 cut(s) | 730 |
| SaqAI | TTAA | 2 cut(s) | 638, 1010 |
| Sau3AI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| ScaI | AGTACT | 1 cut(s) | 663 |
| ScrFI | CCNGG | 1 cut(s) | 107 |
| SduI | GDGCHC | 6 cut(s) | 68, 132, 615, 730, 853, 971 |
| SfaNI | GCATC | 3 cut(s) | 151, 427, 754 |
| SfcI | CTRYAG | 2 cut(s) | 366, 1242 |
| Sfr274I | CTCGAG | 1 cut(s) | 825 |
| SfuI | TTCGAA | 1 cut(s) | 1066 |
| SlaI | CTCGAG | 1 cut(s) | 825 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 624 |
| SmlI | CTYRAG | 1 cut(s) | 825 |
| SmoI | CTYRAG | 1 cut(s) | 825 |
| Sse9I | AATT | 4 cut(s) | 668, 761, 807, 1043 |
| SsiI | CCGC | 2 cut(s) | 235, 401 |
| SspI | AATATT | 1 cut(s) | 226 |
| SspMI | CTAG | 4 cut(s) | 513, 644, 665, 1031 |
| SstI | GAGCTC | 1 cut(s) | 730 |
| StyD4I | CCNGG | 1 cut(s) | 105 |
| StyI | CCWWGG | 1 cut(s) | 731 |
| TaaI | ACNGT | 2 cut(s) | 310, 661 |
| TaiI | ACGT | 1 cut(s) | 12 |
| TaqI | TCGA | 3 cut(s) | 60, 826, 1066 |
| TasI | AATT | 4 cut(s) | 668, 761, 807, 1043 |
| TatI | WGTACW | 2 cut(s) | 661, 943 |
| TfiI | GAWTC | 3 cut(s) | 139, 476, 800 |
| Tru1I | TTAA | 2 cut(s) | 638, 1010 |
| Tru9I | TTAA | 2 cut(s) | 638, 1010 |
| TscAI | CASTG | 5 cut(s) | 319, 399, 802, 1081, 1156 |
| TseFI | GTSAC | 1 cut(s) | 956 |
| Tsp45I | GTSAC | 1 cut(s) | 956 |
| TspDTI | ATGAA | 5 cut(s) | 22, 336, 453, 570, 600 |
| TspRI | CASTG | 5 cut(s) | 319, 399, 802, 1081, 1156 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| XapI | RAATTY | 2 cut(s) | 668, 1043 |
| XbaI | TCTAGA | 1 cut(s) | 512 |
| XceI | RCATGY | 4 cut(s) | 433, 929, 1097, 1183 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 283 |
| XhoI | CTCGAG | 1 cut(s) | 825 |
| XmnI | GAANNNNTTC | 2 cut(s) | 29, 561 |
| XspI | CTAG | 4 cut(s) | 513, 644, 665, 1031 |
| ZrmI | AGTACT | 1 cut(s) | 663 |