Belongs to the eIF-2B alpha beta delta subunits family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1246 |
| Acc65I | GGTACC | 1 cut(s) | 127 |
| AccB1I | GGYRCC | 2 cut(s) | 127, 149 |
| AccBSI | CCGCTC | 1 cut(s) | 1087 |
| AccI | GTMKAC | 1 cut(s) | 305 |
| AciI | CCGC | 3 cut(s) | 92, 124, 1085 |
| AclWI | GGATC | 2 cut(s) | 270, 1008 |
| AcoI | YGGCCR | 1 cut(s) | 864 |
| AcsI | RAATTY | 2 cut(s) | 668, 1043 |
| AcuI | CTGAAG | 3 cut(s) | 501, 548, 1011 |
| AfaI | GTAC | 4 cut(s) | 129, 663, 945, 1184 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| AflIII | ACRYGT | 2 cut(s) | 300, 1179 |
| AgsI | TTSAA | 5 cut(s) | 298, 513, 545, 654, 898 |
| AjiI | CACGTC | 1 cut(s) | 10 |
| AjnI | CCWGG | 1 cut(s) | 18 |
| Alw21I | GWGCWC | 4 cut(s) | 339, 392, 615, 730 |
| AlwI | GGATC | 2 cut(s) | 270, 1008 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 446 |
| AoxI | GGCC | 3 cut(s) | 72, 742, 864 |
| ApoI | RAATTY | 2 cut(s) | 668, 1043 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 29, 99 |
| Asp718I | GGTACC | 1 cut(s) | 127 |
| AspLEI | GCGC | 2 cut(s) | 851, 919 |
| AspS9I | GGNCC | 3 cut(s) | 105, 742, 1225 |
| AsuHPI | GGTGA | 4 cut(s) | 34, 344, 485, 611 |
| AvaII | GGWCC | 2 cut(s) | 105, 1225 |
| BaeGI | GKGCMC | 1 cut(s) | 971 |
| BalI | TGGCCA | 1 cut(s) | 866 |
| BanI | GGYRCC | 2 cut(s) | 127, 149 |
| BanII | GRGCYC | 2 cut(s) | 68, 730 |
| BbsI | GAAGAC | 1 cut(s) | 1240 |
| Bbv12I | GWGCWC | 4 cut(s) | 339, 392, 615, 730 |
| BccI | CCATC | 5 cut(s) | 323, 354, 412, 753, 1180 |
| BceAI | ACGGC | 2 cut(s) | 105, 914 |
| BciT130I | CCWGG | 1 cut(s) | 20 |
| BciVI | GTATCC | 1 cut(s) | 987 |
| BclI | TGATCA | 1 cut(s) | 792 |
| BfaI | CTAG | 1 cut(s) | 752 |
| BfmI | CTRYAG | 2 cut(s) | 276, 1242 |
| BfoI | RGCGCY | 1 cut(s) | 852 |
| BfuAI | ACCTGC | 1 cut(s) | 1246 |
| BfuI | GTATCC | 1 cut(s) | 987 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 602 |
| BmcAI | AGTACT | 1 cut(s) | 663 |
| Bme1390I | CCNGG | 1 cut(s) | 20 |
| Bme18I | GGWCC | 2 cut(s) | 105, 1225 |
| BmgBI | CACGTC | 1 cut(s) | 10 |
| BmgT120I | GGNCC | 3 cut(s) | 105, 742, 1225 |
| BmiI | GGNNCC | 2 cut(s) | 129, 151 |
| BmrFI | CCNGG | 1 cut(s) | 20 |
| BmsI | GCATC | 2 cut(s) | 151, 427 |
| BpiI | GAAGAC | 1 cut(s) | 1240 |
| BpuEI | CTTGAG | 1 cut(s) | 443 |
| BsaJI | CCNNGG | 2 cut(s) | 18, 731 |
| BsaWI | WCCGGW | 1 cut(s) | 349 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| Bse118I | RCCGGY | 2 cut(s) | 950, 1185 |
| Bse1I | ACTGG | 1 cut(s) | 669 |
| BseBI | CCWGG | 1 cut(s) | 20 |
| BseDI | CCNNGG | 2 cut(s) | 18, 731 |
| BseGI | GGATG | 1 cut(s) | 346 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| BseMII | CTCAG | 1 cut(s) | 1014 |
| BseNI | ACTGG | 1 cut(s) | 669 |
| BseRI | GAGGAG | 1 cut(s) | 272 |
| BseSI | GKGCMC | 1 cut(s) | 971 |
| BseYI | CCCAGC | 1 cut(s) | 290 |
| BsgI | GTGCAG | 1 cut(s) | 227 |
| BshFI | GGCC | 3 cut(s) | 74, 744, 866 |
| BshNI | GGYRCC | 2 cut(s) | 127, 149 |
| BsiHKAI | GWGCWC | 4 cut(s) | 339, 392, 615, 730 |
| BsiSI | CCGG | 3 cut(s) | 350, 951, 1186 |
| BslFI | GGGAC | 1 cut(s) | 917 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 628, 1244 |
| BsmFI | GGGAC | 1 cut(s) | 917 |
| BsmI | GAATGC | 1 cut(s) | 13 |
| BsnI | GGCC | 3 cut(s) | 74, 744, 866 |
| Bsp1286I | GDGCHC | 6 cut(s) | 68, 339, 392, 615, 730, 971 |
| Bsp143I | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| BspACI | CCGC | 3 cut(s) | 92, 124, 1085 |
| BspANI | GGCC | 3 cut(s) | 74, 744, 866 |
| BspCNI | CTCAG | 1 cut(s) | 1015 |
| BspLI | GGNNCC | 2 cut(s) | 129, 151 |
| BspMI | ACCTGC | 1 cut(s) | 1246 |
| BspPI | GGATC | 2 cut(s) | 270, 1008 |
| BspT107I | GGYRCC | 2 cut(s) | 127, 149 |
| BsrBI | CCGCTC | 1 cut(s) | 1087 |
| BsrFI | RCCGGY | 2 cut(s) | 950, 1185 |
| BsrI | ACTGG | 1 cut(s) | 669 |
| BssAI | RCCGGY | 2 cut(s) | 950, 1185 |
| BssECI | CCNNGG | 2 cut(s) | 18, 731 |
| BssMI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| BssT1I | CCWWGG | 1 cut(s) | 731 |
| Bst2UI | CCWGG | 1 cut(s) | 20 |
| Bst4CI | ACNGT | 2 cut(s) | 310, 661 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 812 |
| BstC8I | GCNNGC | 4 cut(s) | 213, 603, 927, 952 |
| BstDEI | CTNAG | 1 cut(s) | 1023 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| BstF5I | GGATG | 1 cut(s) | 346 |
| BstH2I | RGCGCY | 1 cut(s) | 852 |
| BstHHI | GCGC | 2 cut(s) | 851, 919 |
| BstKTI | GATC | 4 cut(s) | 265, 701, 795, 1016 |
| BstMBI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| BstNI | CCWGG | 1 cut(s) | 20 |
| BstNSI | RCATGY | 4 cut(s) | 304, 433, 929, 1183 |
| BstSCI | CCNGG | 1 cut(s) | 18 |
| BstSFI | CTRYAG | 2 cut(s) | 276, 1242 |
| BstSLI | GKGCMC | 1 cut(s) | 971 |
| BstV2I | GAAGAC | 1 cut(s) | 1240 |
| BstX2I | RGATCY | 2 cut(s) | 262, 1013 |
| BstYI | RGATCY | 2 cut(s) | 262, 1013 |
| BsuI | GTATCC | 1 cut(s) | 987 |
| BsuRI | GGCC | 3 cut(s) | 74, 744, 866 |
| BtgZI | GCGATG | 1 cut(s) | 1177 |
| BtrI | CACGTC | 1 cut(s) | 10 |
| BtsCI | GGATG | 1 cut(s) | 346 |
| BtsI | GCAGTG | 2 cut(s) | 392, 1074 |
| BtsIMutI | CAGTG | 4 cut(s) | 392, 795, 1074, 1149 |
| BveI | ACCTGC | 1 cut(s) | 1246 |
| Cac8I | GCNNGC | 4 cut(s) | 213, 603, 927, 952 |
| CaiI | CAGNNNCTG | 1 cut(s) | 446 |
| CfoI | GCGC | 2 cut(s) | 851, 919 |
| Cfr10I | RCCGGY | 2 cut(s) | 950, 1185 |
| Cfr13I | GGNCC | 3 cut(s) | 105, 742, 1225 |
| CpoI | CGGWCCG | 1 cut(s) | 105 |
| CseI | GACGC | 1 cut(s) | 386 |
| Csp6I | GTAC | 4 cut(s) | 128, 662, 944, 1183 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 390, 425 |
| CspI | CGGWCCG | 1 cut(s) | 105 |
| CviQI | GTAC | 4 cut(s) | 128, 662, 944, 1183 |
| DdeI | CTNAG | 1 cut(s) | 1023 |
| DpnI | GATC | 4 cut(s) | 264, 700, 794, 1015 |
| DpnII | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| EaeI | YGGCCR | 1 cut(s) | 864 |
| EciI | GGCGGA | 1 cut(s) | 107 |
| Ecl136II | GAGCTC | 1 cut(s) | 728 |
| Eco130I | CCWWGG | 1 cut(s) | 731 |
| Eco24I | GRGCYC | 2 cut(s) | 68, 730 |
| Eco32I | GATATC | 1 cut(s) | 822 |
| Eco47I | GGWCC | 2 cut(s) | 105, 1225 |
| Eco53kI | GAGCTC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 3 cut(s) | 501, 548, 1011 |
| EcoICRI | GAGCTC | 1 cut(s) | 728 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| EcoRI | GAATTC | 1 cut(s) | 1043 |
| EcoRII | CCWGG | 1 cut(s) | 18 |
| EcoRV | GATATC | 1 cut(s) | 822 |
| EcoT14I | CCWWGG | 1 cut(s) | 731 |
| EcoT38I | GRGCYC | 2 cut(s) | 68, 730 |
| ErhI | CCWWGG | 1 cut(s) | 731 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1003, 1035 |
| FaqI | GGGAC | 1 cut(s) | 917 |
| FauI | CCCGC | 2 cut(s) | 117, 1092 |
| FbaI | TGATCA | 1 cut(s) | 792 |
| FblI | GTMKAC | 1 cut(s) | 305 |
| FokI | GGATG | 1 cut(s) | 333 |
| FriOI | GRGCYC | 2 cut(s) | 68, 730 |
| FspBI | CTAG | 1 cut(s) | 752 |
| GlaI | GCGC | 2 cut(s) | 850, 918 |
| GsaI | CCCAGC | 1 cut(s) | 294 |
| HaeII | RGCGCY | 1 cut(s) | 852 |
| HaeIII | GGCC | 3 cut(s) | 74, 744, 866 |
| HapII | CCGG | 3 cut(s) | 350, 951, 1186 |
| HgaI | GACGC | 1 cut(s) | 386 |
| HhaI | GCGC | 2 cut(s) | 851, 919 |
| Hin6I | GCGC | 2 cut(s) | 849, 917 |
| HinP1I | GCGC | 2 cut(s) | 849, 917 |
| HincII | GTYRAC | 1 cut(s) | 306 |
| HindII | GTYRAC | 1 cut(s) | 306 |
| HindIII | AAGCTT | 2 cut(s) | 41, 1130 |
| HinfI | GANTC | 2 cut(s) | 476, 800 |
| HpaII | CCGG | 3 cut(s) | 350, 951, 1186 |
| HphI | GGTGA | 4 cut(s) | 34, 344, 485, 611 |
| Hpy166II | GTNNAC | 3 cut(s) | 306, 401, 832 |
| Hpy188I | TCNGA | 4 cut(s) | 481, 522, 1024, 1051 |
| Hpy188III | TCNNGA | 4 cut(s) | 422, 513, 825, 989 |
| Hpy8I | GTNNAC | 3 cut(s) | 306, 401, 832 |
| HpyAV | CCTTC | 2 cut(s) | 1029, 1100 |
| HpyCH4III | ACNGT | 2 cut(s) | 310, 661 |
| HpyCH4IV | ACGT | 2 cut(s) | 9, 52 |
| HpyF3I | CTNAG | 1 cut(s) | 1023 |
| HpySE526I | ACGT | 2 cut(s) | 9, 52 |
| HspAI | GCGC | 2 cut(s) | 849, 917 |
| KpnI | GGTACC | 1 cut(s) | 131 |
| KroI | GCCGGC | 1 cut(s) | 950 |
| KroNI | GCCGGC | 1 cut(s) | 952 |
| Ksp22I | TGATCA | 1 cut(s) | 792 |
| Kzo9I | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| LmnI | GCTCC | 5 cut(s) | 181, 387, 725, 733, 1092 |
| LweI | GCATC | 2 cut(s) | 151, 427 |
| MaeI | CTAG | 1 cut(s) | 752 |
| MaeII | ACGT | 2 cut(s) | 9, 52 |
| MalI | GATC | 4 cut(s) | 264, 700, 794, 1015 |
| MbiI | CCGCTC | 1 cut(s) | 1087 |
| MboI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| MboII | GAAGA | 5 cut(s) | 335, 453, 494, 740, 1245 |
| MflI | RGATCY | 2 cut(s) | 262, 1013 |
| MhlI | GDGCHC | 6 cut(s) | 68, 339, 392, 615, 730, 971 |
| MlsI | TGGCCA | 1 cut(s) | 866 |
| MluCI | AATT | 4 cut(s) | 95, 668, 761, 1043 |
| MluNI | TGGCCA | 1 cut(s) | 866 |
| MmeI | TCCRAC | 2 cut(s) | 500, 1252 |
| Mox20I | TGGCCA | 1 cut(s) | 866 |
| MroNI | GCCGGC | 1 cut(s) | 950 |
| MroXI | GAANNNNTTC | 2 cut(s) | 29, 99 |
| MscI | TGGCCA | 1 cut(s) | 866 |
| MseI | TTAA | 4 cut(s) | 45, 168, 638, 1010 |
| MslI | CAYNNNNRTG | 1 cut(s) | 624 |
| Msp20I | TGGCCA | 1 cut(s) | 866 |
| MspA1I | CMGCKG | 4 cut(s) | 124, 187, 281, 446 |
| MspI | CCGG | 3 cut(s) | 350, 951, 1186 |
| MspR9I | CCNGG | 1 cut(s) | 20 |
| Mva1269I | GAATGC | 1 cut(s) | 13 |
| MvaI | CCWGG | 1 cut(s) | 20 |
| NaeI | GCCGGC | 1 cut(s) | 952 |
| NdeII | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| NgoMIV | GCCGGC | 1 cut(s) | 950 |
| NlaIV | GGNNCC | 2 cut(s) | 129, 151 |
| NmeAIII | GCCGAG | 1 cut(s) | 622 |
| NspI | RCATGY | 4 cut(s) | 304, 433, 929, 1183 |
| PaeI | GCATGC | 1 cut(s) | 929 |
| PciI | ACATGT | 2 cut(s) | 300, 1179 |
| PctI | GAATGC | 1 cut(s) | 13 |
| PdiI | GCCGGC | 1 cut(s) | 952 |
| PdmI | GAANNNNTTC | 2 cut(s) | 29, 99 |
| PfeI | GAWTC | 2 cut(s) | 476, 800 |
| PscI | ACATGT | 2 cut(s) | 300, 1179 |
| Psp124BI | GAGCTC | 1 cut(s) | 730 |
| Psp6I | CCWGG | 1 cut(s) | 18 |
| PspFI | CCCAGC | 1 cut(s) | 290 |
| PspGI | CCWGG | 1 cut(s) | 18 |
| PspN4I | GGNNCC | 2 cut(s) | 129, 151 |
| PspPI | GGNCC | 3 cut(s) | 105, 742, 1225 |
| PstNI | CAGNNNCTG | 1 cut(s) | 446 |
| PsuI | RGATCY | 2 cut(s) | 262, 1013 |
| PvuII | CAGCTG | 3 cut(s) | 187, 281, 446 |
| RsaI | GTAC | 4 cut(s) | 129, 663, 945, 1184 |
| RsaNI | GTAC | 4 cut(s) | 128, 662, 944, 1183 |
| RseI | CAYNNNNRTG | 1 cut(s) | 624 |
| Rsr2I | CGGWCCG | 1 cut(s) | 105 |
| RsrII | CGGWCCG | 1 cut(s) | 105 |
| SacI | GAGCTC | 1 cut(s) | 730 |
| SalI | GTCGAC | 1 cut(s) | 304 |
| SaqAI | TTAA | 4 cut(s) | 45, 168, 638, 1010 |
| Sau3AI | GATC | 4 cut(s) | 262, 698, 792, 1013 |
| Sau96I | GGNCC | 3 cut(s) | 105, 742, 1225 |
| ScaI | AGTACT | 1 cut(s) | 663 |
| ScrFI | CCNGG | 1 cut(s) | 20 |
| SduI | GDGCHC | 6 cut(s) | 68, 339, 392, 615, 730, 971 |
| SfaNI | GCATC | 2 cut(s) | 151, 427 |
| SfcI | CTRYAG | 2 cut(s) | 276, 1242 |
| SinI | GGWCC | 2 cut(s) | 105, 1225 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 624 |
| SmlI | CTYRAG | 1 cut(s) | 422 |
| SmoI | CTYRAG | 1 cut(s) | 422 |
| SphI | GCATGC | 1 cut(s) | 929 |
| Sse9I | AATT | 4 cut(s) | 95, 668, 761, 1043 |
| SsiI | CCGC | 3 cut(s) | 92, 124, 1085 |
| SspI | AATATT | 2 cut(s) | 58, 226 |
| SspMI | CTAG | 1 cut(s) | 752 |
| SstI | GAGCTC | 1 cut(s) | 730 |
| StyD4I | CCNGG | 1 cut(s) | 18 |
| StyI | CCWWGG | 1 cut(s) | 731 |
| TaaI | ACNGT | 2 cut(s) | 310, 661 |
| TaiI | ACGT | 2 cut(s) | 12, 55 |
| TaqI | TCGA | 1 cut(s) | 305 |
| TaqII | GACCGA | 1 cut(s) | 93 |
| TasI | AATT | 4 cut(s) | 95, 668, 761, 1043 |
| TatI | WGTACW | 2 cut(s) | 661, 943 |
| TfiI | GAWTC | 2 cut(s) | 476, 800 |
| Tru1I | TTAA | 4 cut(s) | 45, 168, 638, 1010 |
| Tru9I | TTAA | 4 cut(s) | 45, 168, 638, 1010 |
| TscAI | CASTG | 4 cut(s) | 399, 802, 1081, 1156 |
| TspDTI | ATGAA | 6 cut(s) | 105, 336, 453, 570, 600, 1082 |
| TspGWI | ACGGA | 1 cut(s) | 97 |
| TspRI | CASTG | 4 cut(s) | 399, 802, 1081, 1156 |
| VpaK11BI | GGWCC | 2 cut(s) | 105, 1225 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1242 |
| XapI | RAATTY | 2 cut(s) | 668, 1043 |
| XceI | RCATGY | 4 cut(s) | 304, 433, 929, 1183 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 832 |
| XmiI | GTMKAC | 1 cut(s) | 305 |
| XmnI | GAANNNNTTC | 2 cut(s) | 29, 99 |
| XspI | CTAG | 1 cut(s) | 752 |
| ZrmI | AGTACT | 1 cut(s) | 663 |