MD13G1218200.v1.1

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000605
Physical Location & Seq
Forward (+)
20962764 .. 20964611
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1218200.v1.1.491

Sequence Viewer

Length: 702 bp
ATGAACAGATTGCGGATCTGGCGGTGGAGCTTATACATCAAAAGTAATTTTCCCTTAAACCGTGAACATGTTTTTGCTACTGATGAGGTGGTACTAACCTTAGGTCAGTCAAGAACCGTGAAAAAGTTCCTATGTGCTGCGAAGAAGAAACGATCATTCCATGTCTTTGTTGCTGAGGGTGCTCCAAAGTATCTGGGTCATGTTCTTGCAAAAGCGTTGGCTGCAAAAGGTTTGCAAACTACAATGATTACCGACTCTTCAGTTTTCGCCATGATTTCACGAGTAAACGTTGTCATAGTCGGGGTTCATGCCGTGATGGCCAATGGTGGGATCATAGCTCCTGTTGGGACGAATATGGTTGCACTTGCTGCAAAAAAGCATGCTGTTCCGTTTGTTGTGGTTGCTGGCACTCATAAGTTGTGTCCACTATATCCTAATAATCCAGAGGTTTTGCTGAATGATATGAGATGCCCATCAGAACTCTTATCTTTTGGGGAATTCTCAGATTTCGTGGATTTTAGTATCGGCAGTGGAGCTTCTCTTCTTAACATTGTTAATCCTGCATTTGATTATGTGCCACCGGAGCTAGTCAGCCTTTTTGTTACTGAAATAGGGGGGCACAATCCATCATATATCTATAGGCTCATTTCAGATTTTTACTGTGCTGATGATTTGCCTCGACAACAAAAACCGGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IF-2B

Protein Analysis

234

Amino Acids

25.58

Weight (kDa)

9.17

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 24 - 209 1.9e-49 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 13, 22
AclI AACGTT 1 cut(s) 288
AclWI GGATC 2 cut(s) 23, 338
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 497
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 327
AflIII ACRYGT 1 cut(s) 67
AluBI AGCT 4 cut(s) 30, 338, 536, 586
AluI AGCT 4 cut(s) 30, 338, 536, 586
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 2 cut(s) 23, 338
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 3 cut(s) 137, 221, 368
ApoI RAATTY 1 cut(s) 497
Asp700I GAANNNNTTC 1 cut(s) 125
AxyI CCTNAGG 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 621
BalI TGGCCA 1 cut(s) 320
BauI CACGAG 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 184
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 124, 208, 355
BccI CCATC 3 cut(s) 310, 481, 634
BceAI ACGGC 1 cut(s) 296
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 637
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 3 cut(s) 138, 222, 369
BlsI GCNGC 3 cut(s) 139, 223, 370
BmsI GCATC 1 cut(s) 458
Bpu10I CCTNAGC 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 472
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse118I RCCGGY 1 cut(s) 691
Bse21I CCTNAGG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 472
BseJI GATNNNNATC 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 165, 516
BseSI GKGCMC 1 cut(s) 621
BseXI GCAGC 3 cut(s) 124, 208, 355
BshFI GGCC 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 2 cut(s) 581, 692
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 184, 621
Bsp143I GATC 3 cut(s) 15, 152, 330
BspACI CCGC 2 cut(s) 13, 22
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 166, 515
BspPI GGATC 2 cut(s) 23, 338
BsrFI RCCGGY 1 cut(s) 691
BssAI RCCGGY 1 cut(s) 691
BssMI GATC 3 cut(s) 15, 152, 330
BssSI CACGAG 1 cut(s) 279
Bst2BI CACGAG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 62, 118, 662
Bst6I CTCTTC 2 cut(s) 262, 546
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 381, 406
BstDEI CTNAG 3 cut(s) 100, 174, 502
BstKTI GATC 3 cut(s) 18, 155, 333
BstMBI GATC 3 cut(s) 15, 152, 330
BstMWI GCNNNNNNNGC 6 cut(s) 19, 179, 221, 317, 368, 583
BstNSI RCATGY 2 cut(s) 71, 383
BstSFI CTRYAG 1 cut(s) 637
BstSLI GKGCMC 1 cut(s) 621
BstV1I GCAGC 3 cut(s) 124, 208, 355
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 100
BsuRI GGCC 1 cut(s) 320
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 2 cut(s) 381, 406
Cfr10I RCCGGY 1 cut(s) 691
Csp6I GTAC 1 cut(s) 92
CviAII CATG 6 cut(s) 68, 161, 200, 271, 308, 380
CviJI RGCY 9 cut(s) 30, 221, 320, 338, 536, 586, 594, 643, 695
CviKI_1 RGCY 9 cut(s) 30, 221, 320, 338, 536, 586, 594, 643, 695
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 3 cut(s) 100, 174, 502
DpnI GATC 3 cut(s) 17, 154, 332
DpnII GATC 3 cut(s) 15, 152, 330
EaeI YGGCCR 1 cut(s) 318
Eam1104I CTCTTC 2 cut(s) 262, 546
EarI CTCTTC 2 cut(s) 262, 546
Eco57I CTGAAG 1 cut(s) 243
Eco81I CCTNAGG 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 497
FaeI CATG 6 cut(s) 71, 164, 203, 274, 311, 383
FaqI GGGAC 1 cut(s) 361
FatI CATG 6 cut(s) 67, 160, 199, 270, 307, 379
Fnu4HI GCNGC 3 cut(s) 138, 222, 369
Fsp4HI GCNGC 3 cut(s) 138, 222, 369
FspBI CTAG 1 cut(s) 587
GluI GCNGC 3 cut(s) 138, 222, 369
HaeIII GGCC 1 cut(s) 320
HapII CCGG 2 cut(s) 581, 692
Hin1II CATG 6 cut(s) 71, 164, 203, 274, 311, 383
HinfI GANTC 1 cut(s) 254
HpaII CCGG 2 cut(s) 581, 692
Hpy166II GTNNAC 3 cut(s) 65, 286, 425
Hpy188I TCNGA 3 cut(s) 478, 505, 652
Hpy188III TCNNGA 4 cut(s) 111, 279, 443, 699
Hpy8I GTNNAC 3 cut(s) 65, 286, 425
HpyCH4III ACNGT 3 cut(s) 62, 118, 662
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 6 cut(s) 209, 224, 235, 362, 371, 563
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 179, 221, 317, 368, 583
HpyF3I CTNAG 3 cut(s) 100, 174, 502
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 6 cut(s) 71, 164, 203, 274, 311, 383
Kzo9I GATC 3 cut(s) 15, 152, 330
LmnI GCTCC 5 cut(s) 27, 187, 343, 533, 583
LpnPI CCDG 7 cut(s) 4, 179, 354, 390, 456, 573, 594
Lsp1109I GCAGC 3 cut(s) 124, 208, 355
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 601
MalI GATC 3 cut(s) 17, 154, 332
MboI GATC 3 cut(s) 15, 152, 330
MboII GAAGA 4 cut(s) 154, 157, 249, 533
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 2 cut(s) 184, 621
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 46, 497
MluNI TGGCCA 1 cut(s) 320
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 4 cut(s) 79, 169, 439, 687
Mox20I TGGCCA 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 125
MscI TGGCCA 1 cut(s) 320
MseI TTAA 3 cut(s) 56, 546, 555
Msp20I TGGCCA 1 cut(s) 320
MspI CCGG 2 cut(s) 581, 692
MwoI GCNNNNNNNGC 6 cut(s) 19, 179, 221, 317, 368, 583
NdeII GATC 3 cut(s) 15, 152, 330
NlaIII CATG 6 cut(s) 71, 164, 203, 274, 311, 383
NspI RCATGY 2 cut(s) 71, 383
PaeI GCATGC 1 cut(s) 383
PciI ACATGT 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 125
PkrI GCNGC 3 cut(s) 139, 223, 370
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PscI ACATGT 1 cut(s) 67
Psp1406I AACGTT 1 cut(s) 288
PsuI RGATCY 1 cut(s) 15
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 56, 546, 555
SatI GCNGC 3 cut(s) 138, 222, 369
Sau3AI GATC 3 cut(s) 15, 152, 330
SchI GAGTC 1 cut(s) 248
SduI GDGCHC 2 cut(s) 184, 621
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 637
SphI GCATGC 1 cut(s) 383
Sse9I AATT 2 cut(s) 46, 497
SsiI CCGC 2 cut(s) 13, 22
SspMI CTAG 1 cut(s) 587
TaaI ACNGT 3 cut(s) 62, 118, 662
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 679
TasI AATT 2 cut(s) 46, 497
Tru1I TTAA 3 cut(s) 56, 546, 555
Tru9I TTAA 3 cut(s) 56, 546, 555
TscAI CASTG 1 cut(s) 535
TseI GCWGC 3 cut(s) 137, 221, 368
TspDTI ATGAA 2 cut(s) 17, 296
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 1 cut(s) 535
XapI RAATTY 1 cut(s) 497
XceI RCATGY 2 cut(s) 71, 383
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.