Rh4DG049700

Belongs to the eIF-2B alpha beta delta subunits family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000605
Physical Location & Seq
Forward (+)
7975685 .. 7981607
5923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG049700.1

Sequence Viewer

Length: 1161 bp
ATGGCTGAAGTTCAAGCTCTCGTCAATGAACTCGCCACCAAGCTCCGGAAACGGAAGGTGGAGGGTTCACGAGAGACTGCTCGGCTGACGGCGGAGTTGTTACGGACGGTAATATCCCAGCAGAGGGTGACTTCAGCCGCCCTTGTTGAGGTCGTCAGAGATGTTGGTCAGCAGCTTATTGCTGCTAACCCTGTTGAGCTTGCTGTTGGGAACATTGTGAGGCGTGTTCTGCGCATCATTAGGGATGGGGATCTTTCACCGGAAGTTGGAGGATTGACTTTAGTCTCCCAGGGTGATGATGGAGATATTGTTGAGGAAGATGATAAACCATTTCTATTATCTGATGCTATTTCCCAAACAATTCTCCATCCACCTTCCGTGGTTGCACAGCTTGAAAGCAAACCCAACTTGGTTTGCCACTTTGAGGGAAAAGCAGATAAAACTCCGAGTAGCTGGCAATTTAAGCACCAGATCATTGAGGATATAAATACTTGCCATGAACTGATCGCAGACCAGGCATTGGAGCTTATACATCAAAATGAGGTGATACTAACCTTAGGTCACTCGAAGACTGTCAAGAAGTTCCTCTATGCTGCAAAGGAGAAGAAAAGATCTTTCCAGGTCTATGTGGCCGAGGGTGCTCCTAAGCATTTGGGGCATGTGCTTGCAAATGAGTTAACTGAAAAGCATTTGCAAACAACCGTCATTCCTGACTCTTCAGTGTTTGCCATGATTTCAAGAGTTAACATGGTCATAGTCGGGGTTCATGCTGTGATGGCCAATGGTGGGGTCATAGCCCCTGTTGGGATGAATATGGTTGCACTTGCTGCAAAAAGGCATGCTGTTCCATTTGTTGTGGTTGGTGGCACTCATAAGTTATGTCCACTGTTTCCTAACAATCCAGAGGTTTTGCTGAACGATATGAGATGTCCATCTGAGCTATTATCTTTTGGGGACTTCTCAGATTGCATGGATTTTAGTGTTGGCAGTGGATCTCCTCTTTTGCATGTTGTTAATCCTGCATTTGACTATGTGCCACCCGAGCTAGTCAGTCTTTTTGTTACTGAAATAGGGGGCTATCATCCATCATACATCTATAGGCTCATTTCGGATTATTACTCTGCTGACGATATAGTTCTGCAACAAAAACCTGCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
IF-2B

Protein Analysis

386

Amino Acids

42.22

Weight (kDa)

5.78

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF-2B PF01008 19 - 362 9.7e-61 Initiation factor 2 subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000605)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07300 AT3G07300 AT3G07300
fragaria_vesca FvH4_1g26370 FvH4_4g04150 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780 FvH4_7g02780
malus_domestica MD02G1290200.v1.1 MD07G1036800.v1.1 MD13G1218100.v1.1 MD13G1218200.v1.1
prunus_persica Prupe.1G044800_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1 Prupe.2G032300_v2.0.a1
pyrus_communis pycom07g02550
rosa_chinensis RchiOBHm_Chr1g0313651 RchiOBHm_Chr1g0322021 RchiOBHm_Chr1g0322871 RchiOBHm_Chr1g0322881 RchiOBHm_Chr1g0378031 RchiOBHm_Chr2g0146721 RchiOBHm_Chr4g0394571 RchiOBHm_Chr4g0444661
rosa_laevigata RLG00000009689 RLG00000014710 RLG00000020215 RLG00000028674 RLG00000030393
rosa_multiflora Rmu_sc0001168.1_g000008 Rmu_sc0001168.1_g000019 Rmu_sc0001986.1_g000042 Rmu_sc0005424.1_g000018
rosa_roxburghii Rroxscaffold_1G00066950 Rroxscaffold_4G00308160 Rroxscaffold_4G00327340 Rroxscaffold_4G00331500 Rroxscaffold_5G00339610 Rroxscaffold_5G00370190 Rroxscaffold_6G00400520 Rroxscaffold_7G00188200
rosa_rugosa Rorug01G0035100 Rorug01G0035200 Rorug01G0131800.1 Rorug01G0193700 Rorug03G0291700 Rorug03G0291800 Rorug03G0291800 Rorug03G0291900 Rorug03G0364400.1 Rorug04G0439300 Rorug04G0439400 Rorug05G0543000 Rorug07G0306800
rosa_samantha Rh1AG048400 Rh1AG309400 Rh1AG358100 Rh1AG424800 Rh1BG046200 Rh1CG052500 Rh1DG056700 Rh2AG452600 Rh2BG465300 Rh2CG439500 Rh2DG245700 Rh2DG245800 Rh2DG474300 Rh4AG053000 Rh4BG050900 Rh4CG057700 Rh4DG049700 Rh5BG052500 Rh5DG543800 Rh6DG164300 Rh7AG422900 Rh7BG328900
rosa_wichuraiana Rw1G004380 Rw4G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 520
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 2 cut(s) 92, 138
AclWI GGATC 2 cut(s) 258, 1000
AcoI YGGCCR 2 cut(s) 630, 777
AcuI CTGAAG 3 cut(s) 27, 117, 702
AfiI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 520, 786, 804
AgsI TTSAA 3 cut(s) 14, 395, 738
AjnI CCWGG 3 cut(s) 288, 513, 618
AluBI AGCT 9 cut(s) 17, 43, 175, 199, 391, 453, 526, 940, 1045
AluI AGCT 9 cut(s) 17, 43, 175, 199, 391, 453, 526, 940, 1045
Alw21I GWGCWC 1 cut(s) 643
Alw26I GTCTC 2 cut(s) 68, 289
AlwI GGATC 2 cut(s) 258, 1000
Ama87I CYCGRG 1 cut(s) 1040
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 2 cut(s) 630, 777
ApeKI GCWGC 4 cut(s) 172, 182, 593, 827
AspLEI GCGC 1 cut(s) 234
AsuHPI GGTGA 4 cut(s) 139, 249, 305, 556
AvaI CYCGRG 1 cut(s) 1040
AxyI CCTNAGG 1 cut(s) 556
BalI TGGCCA 1 cut(s) 779
BauI CACGAG 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 643
BbvI GCAGC 4 cut(s) 169, 184, 580, 814
BccI CCATC 6 cut(s) 239, 293, 375, 769, 940, 1093
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 3 cut(s) 290, 515, 620
BcoDI GTCTC 2 cut(s) 68, 289
BfaI CTAG 1 cut(s) 1046
BfmI CTRYAG 1 cut(s) 1096
BglII AGATCT 1 cut(s) 611
BisI GCNGC 5 cut(s) 138, 173, 183, 594, 828
BlsI GCNGC 5 cut(s) 139, 174, 184, 595, 829
Bme1390I CCNGG 3 cut(s) 290, 515, 620
BmeT110I CYCGRG 1 cut(s) 1040
BmrFI CCNGG 3 cut(s) 290, 515, 620
BmsI GCATC 2 cut(s) 243, 334
BoxI GACNNNNGTC 1 cut(s) 281
BpiI GAAGAC 1 cut(s) 575
Bpu10I CCTNAGC 1 cut(s) 645
BsaBI GATNNNNATC 2 cut(s) 249, 931
BsaJI CCNNGG 4 cut(s) 288, 289, 378, 633
BsaWI WCCGGW 2 cut(s) 45, 259
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 520, 786, 804
Bse21I CCTNAGG 1 cut(s) 556
Bse8I GATNNNNATC 2 cut(s) 249, 931
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 3 cut(s) 290, 515, 620
BseDI CCNNGG 4 cut(s) 288, 289, 378, 633
BseGI GGATG 4 cut(s) 250, 367, 813, 1081
BseJI GATNNNNATC 2 cut(s) 249, 931
BseLI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 520, 786, 804
BseMII CTCAG 2 cut(s) 927, 975
BseRI GAGGAG 1 cut(s) 987
BseXI GCAGC 4 cut(s) 169, 184, 580, 814
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 2 cut(s) 632, 779
BsiHKAI GWGCWC 1 cut(s) 643
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 2 cut(s) 46, 260
BslFI GGGAC 1 cut(s) 968
BslI CCNNNNNNNGG 9 cut(s) 45, 123, 124, 148, 266, 424, 520, 786, 804
BsmAI GTCTC 2 cut(s) 68, 289
BsmFI GGGAC 1 cut(s) 968
BsnI GGCC 2 cut(s) 632, 779
BsoBI CYCGRG 1 cut(s) 1040
Bsp1286I GDGCHC 1 cut(s) 643
Bsp13I TCCGGA 1 cut(s) 45
Bsp143I GATC 5 cut(s) 250, 471, 504, 611, 992
BspACI CCGC 2 cut(s) 92, 138
BspANI GGCC 2 cut(s) 632, 779
BspCNI CTCAG 2 cut(s) 928, 974
BspEI TCCGGA 1 cut(s) 45
BspPI GGATC 2 cut(s) 258, 1000
BssECI CCNNGG 4 cut(s) 288, 289, 378, 633
BssMI GATC 5 cut(s) 250, 471, 504, 611, 992
BssSI CACGAG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 3 cut(s) 290, 515, 620
Bst4CI ACNGT 4 cut(s) 109, 574, 703, 888
Bst6I CTCTTC 1 cut(s) 721
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 4 cut(s) 201, 455, 666, 840
BstDEI CTNAG 4 cut(s) 556, 645, 936, 961
BstDSI CCRYGG 1 cut(s) 378
BstENI CCTNNNNNAGG 1 cut(s) 146
BstF5I GGATG 4 cut(s) 250, 367, 813, 1081
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 5 cut(s) 253, 474, 507, 614, 995
BstMAI GTCTC 2 cut(s) 68, 289
BstMBI GATC 5 cut(s) 250, 471, 504, 611, 992
BstMWI GCNNNNNNNGC 8 cut(s) 229, 463, 515, 638, 655, 776, 827, 1042
BstNI CCWGG 3 cut(s) 290, 515, 620
BstNSI RCATGY 3 cut(s) 662, 842, 1010
BstPAI GACNNNNGTC 1 cut(s) 281
BstSCI CCNGG 3 cut(s) 288, 513, 618
BstSFI CTRYAG 1 cut(s) 1096
BstV1I GCAGC 4 cut(s) 169, 184, 580, 814
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 3 cut(s) 250, 611, 992
BstYI RGATCY 3 cut(s) 250, 611, 992
Bsu36I CCTNAGG 1 cut(s) 556
BsuRI GGCC 2 cut(s) 632, 779
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 4 cut(s) 250, 367, 813, 1081
BtsI GCAGTG 1 cut(s) 994
BtsIMutI CAGTG 3 cut(s) 726, 884, 994
Cac8I GCNNGC 4 cut(s) 201, 455, 666, 840
CfoI GCGC 1 cut(s) 234
CviAII CATG 8 cut(s) 497, 659, 730, 748, 767, 839, 970, 1007
DdeI CTNAG 4 cut(s) 556, 645, 936, 961
DpnI GATC 5 cut(s) 252, 473, 506, 613, 994
DpnII GATC 5 cut(s) 250, 471, 504, 611, 992
EaeI YGGCCR 2 cut(s) 630, 777
Eam1104I CTCTTC 1 cut(s) 721
EarI CTCTTC 1 cut(s) 721
EciI GGCGGA 1 cut(s) 107
Eco57I CTGAAG 3 cut(s) 27, 117, 702
Eco81I CCTNAGG 1 cut(s) 556
Eco88I CYCGRG 1 cut(s) 1040
EcoNI CCTNNNNNAGG 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 288, 513, 618
FaeI CATG 8 cut(s) 500, 662, 733, 751, 770, 842, 973, 1010
FaqI GGGAC 1 cut(s) 968
FatI CATG 8 cut(s) 496, 658, 729, 747, 766, 838, 969, 1006
Fnu4HI GCNGC 5 cut(s) 138, 173, 183, 594, 828
FokI GGATG 4 cut(s) 257, 354, 820, 1068
Fsp4HI GCNGC 5 cut(s) 138, 173, 183, 594, 828
FspBI CTAG 1 cut(s) 1046
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 1 cut(s) 233
GluI GCNGC 5 cut(s) 138, 173, 183, 594, 828
GsaI CCCAGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 632, 779
HapII CCGG 2 cut(s) 46, 260
HhaI GCGC 1 cut(s) 234
Hin1II CATG 8 cut(s) 500, 662, 733, 751, 770, 842, 973, 1010
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 2 cut(s) 678, 745
HindII GTYRAC 2 cut(s) 678, 745
HinfI GANTC 1 cut(s) 713
HpaI GTTAAC 2 cut(s) 678, 745
HpaII CCGG 2 cut(s) 46, 260
HphI GGTGA 4 cut(s) 139, 249, 305, 556
Hpy166II GTNNAC 4 cut(s) 68, 678, 745, 884
Hpy188I TCNGA 6 cut(s) 158, 343, 447, 937, 964, 1111
Hpy188III TCNNGA 7 cut(s) 46, 69, 577, 710, 738, 902, 1158
Hpy8I GTNNAC 4 cut(s) 68, 678, 745, 884
HpyAV CCTTC 2 cut(s) 49, 384
HpyCH4III ACNGT 4 cut(s) 109, 574, 703, 888
HpyF10VI GCNNNNNNNGC 8 cut(s) 229, 463, 515, 638, 655, 776, 827, 1042
HpyF3I CTNAG 4 cut(s) 556, 645, 936, 961
Hsp92II CATG 8 cut(s) 500, 662, 733, 751, 770, 842, 973, 1010
HspAI GCGC 1 cut(s) 232
Kpn2I TCCGGA 1 cut(s) 45
KspAI GTTAAC 2 cut(s) 678, 745
Kzo9I GATC 5 cut(s) 250, 471, 504, 611, 992
LmnI GCTCC 3 cut(s) 48, 523, 646
Lsp1109I GCAGC 4 cut(s) 169, 184, 580, 814
LweI GCATC 2 cut(s) 243, 334
MaeI CTAG 1 cut(s) 1046
MaeIII GTNAC 4 cut(s) 99, 127, 560, 1060
MalI GATC 5 cut(s) 252, 473, 506, 613, 994
MboI GATC 5 cut(s) 250, 471, 504, 611, 992
MboII GAAGA 4 cut(s) 329, 580, 616, 708
MflI RGATCY 3 cut(s) 250, 611, 992
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 779
MluCI AATT 2 cut(s) 360, 458
MluNI TGGCCA 1 cut(s) 779
MlyI GAGTC 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 779
MroI TCCGGA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 779
MseI TTAA 4 cut(s) 462, 677, 744, 1014
MslI CAYNNNNRTG 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 779
MspI CCGG 2 cut(s) 46, 260
MspR9I CCNGG 3 cut(s) 290, 515, 620
MvaI CCWGG 3 cut(s) 290, 515, 620
MwoI GCNNNNNNNGC 8 cut(s) 229, 463, 515, 638, 655, 776, 827, 1042
NdeII GATC 5 cut(s) 250, 471, 504, 611, 992
NlaIII CATG 8 cut(s) 500, 662, 733, 751, 770, 842, 973, 1010
NmeAIII GCCGAG 2 cut(s) 61, 658
NmuCI GTSAC 2 cut(s) 127, 560
NsbI TGCGCA 1 cut(s) 233
NspI RCATGY 3 cut(s) 662, 842, 1010
PaeI GCATGC 1 cut(s) 842
PasI CCCWGGG 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 520
PkrI GCNGC 5 cut(s) 139, 174, 184, 595, 829
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
PshAI GACNNNNGTC 1 cut(s) 281
Psp6I CCWGG 3 cut(s) 288, 513, 618
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 3 cut(s) 288, 513, 618
PsuI RGATCY 3 cut(s) 250, 611, 992
RseI CAYNNNNRTG 1 cut(s) 537
SaqAI TTAA 4 cut(s) 462, 677, 744, 1014
SatI GCNGC 5 cut(s) 138, 173, 183, 594, 828
Sau3AI GATC 5 cut(s) 250, 471, 504, 611, 992
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 3 cut(s) 290, 515, 620
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 2 cut(s) 243, 334
SfcI CTRYAG 1 cut(s) 1096
SmiMI CAYNNNNRTG 1 cut(s) 537
SphI GCATGC 1 cut(s) 842
Sse9I AATT 2 cut(s) 360, 458
SsiI CCGC 2 cut(s) 92, 138
SspMI CTAG 1 cut(s) 1046
StyD4I CCNGG 3 cut(s) 288, 513, 618
TaaI ACNGT 4 cut(s) 109, 574, 703, 888
TaqI TCGA 1 cut(s) 566
TasI AATT 2 cut(s) 360, 458
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 4 cut(s) 462, 677, 744, 1014
Tru9I TTAA 4 cut(s) 462, 677, 744, 1014
TscAI CASTG 3 cut(s) 726, 891, 994
TseFI GTSAC 2 cut(s) 127, 560
TseI GCWGC 4 cut(s) 172, 182, 593, 827
Tsp45I GTSAC 2 cut(s) 127, 560
TspDTI ATGAA 4 cut(s) 42, 513, 755, 824
TspGWI ACGGA 3 cut(s) 67, 118, 367
TspRI CASTG 3 cut(s) 726, 891, 994
Van91I CCANNNNNTGG 1 cut(s) 520
XagI CCTNNNNNAGG 1 cut(s) 146
XceI RCATGY 3 cut(s) 662, 842, 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XspI CTAG 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.