FvH4_4g10331

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000949
Physical Location & Seq
Reverse (-)
13816213 .. 13823103
6891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10331.t1

Sequence Viewer

Length: 1377 bp
ATGGCACCACAAGTAACCATTTTCTTCCATAAGCTCCCAGCAACTACTGCTCACCCAAGCCGAATTGGTCATCAAATGTCATTCGTGATTGCCCATGCTCCTGTCACTTCTACAAAGGCAAAGCTTGAAACAAAACAAGACAAGCTAGAAAAGAGGAAGAGGATTGCAAGAGCTCAAAGGAAGAGAGATGCTCAAGCTAAGTCAATTAAAGCCATCCTGGCAGAGATTGCTCAGCCGAAACCTAGTGAGGAAGGAATGAAGATTCCCTCAAGGGAGGTGAAGACTCCCATGCAAGCCTATATCATGGTTGGTATGATGCGGGTCAACCTACCAAGTGTAGAAACCGTGATGGTGACTTCCACCAAGGGAGGTGAAGAAGACAACAAAGAGGGTGAACGTGAACCTCAACTTCCCCTCGAAGGCTCACAAAGGTCCACAATGTCCTCACAGCAAATAACATCTCAAACTAGCCAAGAACTTGATCCAAAGAAGCTAGCAGAGGAGATCTCAAAAGCTCTTGATGCACCAGCAAGAGGTCCAGGGAGGCCGAGGGGATCTAAAACTAGGGAATATAGTGTTTATGGAGATGATTCCTACAAAGGTCCAATTACTCGCAACATGGCTAAAAGGATGATGCTTGATACGTCGTCAGCAAGTACTACGTCAGAAGAAGGATATACGTCTCTAAAGAATAATCCTTTATTTGTCTCGTGGAAGGGTAATCCTTTATATGAGACCTCGACTCCAACTGAAGGCTTCTTACACGTTTTGATACCTCAATCAGTCGCAAGGTCTAGCGAGAGTTGTGATTTTGATTCTGACAATGTTCAAGTTCTCATGGCAAGCTCGTCAAACCTAGAAGAGCAATTGAAACAACAGATGGAGGAGTTGACAAAGAAGTTAGCTCAACTTGAACGCGATGCTCTAGATAGGCAAGTTGAAAGAGAAGCAGAAGCTAAAAGGATAGCAGATATTGAGATCGAGTCGAAGAGGAAATTGGCTGAAAAAGAAGCCGAGGTTGCTGAGCTTATAGCAAAAGTCTACACTCATGACCTCAAAAACACTCGGTCAAAGGATGGACATGCAAGACAAGCTGGTCATTGTGTCTCATTCGTCATTGATCAGGCTTCCATTTCAAGCAACATTTTGAGGTCTAACCATGACGTTAAAGGATGCAGGATTCGTGTGTTTGGTTTAAGGATTATCTTTGAAGGAGTCAAAGACGAAATTGGGTATTTCAACTTGAGATGGGGTGGTGGGATTGATTTTATGCACACCAGAAAAGTGAAAGGAGGAGAACAGCTAGAGGAAGAGTTAGGAGGGGTTGGAAAGCTGCCGAGCCTATTTGGATTGTGGAGATTAGAGCTTAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

459

Amino Acids

51.13

Weight (kDa)

9.02

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1095
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 1041
AccII CGCG 1 cut(s) 918
AciI CCGC 1 cut(s) 319
AclWI GGATC 2 cut(s) 476, 562
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 1 cut(s) 658
AfiI CCNNNNNNNGG 3 cut(s) 419, 533, 752
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 8 cut(s) 128, 830, 871, 914, 941, 1137, 1211, 1240
AjnI CCWGG 2 cut(s) 216, 538
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 4 cut(s) 687, 712, 728, 1111
AlwI GGATC 2 cut(s) 476, 562
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 1 cut(s) 1333
AspS9I GGNCC 3 cut(s) 432, 536, 602
AsuHPI GGTGA 5 cut(s) 44, 289, 364, 383, 404
AsuNHI GCTAGC 1 cut(s) 493
AvaII GGWCC 3 cut(s) 432, 536, 602
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 175
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BauI CACGAG 1 cut(s) 709
BbsI GAAGAC 2 cut(s) 287, 384
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 1 cut(s) 1320
BccI CCATC 5 cut(s) 221, 343, 874, 1070, 1242
BciT130I CCWGG 2 cut(s) 218, 540
BclI TGATCA 1 cut(s) 1120
BcoDI GTCTC 4 cut(s) 687, 712, 728, 1111
BfaI CTAG 9 cut(s) 146, 243, 468, 494, 564, 795, 857, 926, 1304
BfrI CTTAAG 1 cut(s) 1367
BglI GCCNNNNNGGC 1 cut(s) 218
BglII AGATCT 1 cut(s) 504
BisI GCNGC 1 cut(s) 1334
BlpI GCTNAGC 2 cut(s) 231, 1023
BlsI GCNGC 1 cut(s) 1335
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 2 cut(s) 218, 540
Bme18I GGWCC 3 cut(s) 432, 536, 602
BmgT120I GGNCC 3 cut(s) 432, 536, 602
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 218, 540
BmsI GCATC 6 cut(s) 178, 306, 511, 624, 910, 1163
BmtI GCTAGC 1 cut(s) 497
BpiI GAAGAC 2 cut(s) 287, 384
BplI GAGNNNNNCTC 4 cut(s) 175, 207, 491, 523
Bpu1102I GCTNAGC 2 cut(s) 231, 1023
BpuEI CTTGAG 3 cut(s) 177, 253, 1264
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 4 cut(s) 363, 539, 548, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 533, 752
BseBI CCWGG 2 cut(s) 218, 540
BseDI CCNNGG 4 cut(s) 363, 539, 548, 1014
BseGI GGATG 4 cut(s) 213, 636, 1081, 1178
BseLI CCNNNNNNNGG 3 cut(s) 419, 533, 752
BseMII CTCAG 2 cut(s) 245, 1014
BseRI GAGGAG 3 cut(s) 515, 899, 1308
BseXI GCAGC 1 cut(s) 1320
BseYI CCCAGC 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 918
BshFI GGCC 1 cut(s) 547
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 175
BslI CCNNNNNNNGG 3 cut(s) 419, 533, 752
BsmAI GTCTC 4 cut(s) 687, 712, 728, 1111
BsmBI CGTCTC 1 cut(s) 687
BsnI GGCC 1 cut(s) 547
Bso31I GGTCTC 1 cut(s) 728
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 5 cut(s) 481, 504, 554, 978, 1120
Bsp1720I GCTNAGC 2 cut(s) 231, 1023
BspACI CCGC 1 cut(s) 319
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 2 cut(s) 244, 1015
BspFNI CGCG 1 cut(s) 918
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 1 cut(s) 6
BspOI GCTAGC 1 cut(s) 497
BspPI GGATC 2 cut(s) 476, 562
BspQI GCTCTTC 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1367
BspTNI GGTCTC 1 cut(s) 728
BssECI CCNNGG 4 cut(s) 363, 539, 548, 1014
BssMI GATC 5 cut(s) 481, 504, 554, 978, 1120
BssSI CACGAG 1 cut(s) 709
BssT1I CCWWGG 1 cut(s) 363
Bst2BI CACGAG 1 cut(s) 709
Bst2UI CCWGG 2 cut(s) 218, 540
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 5 cut(s) 152, 176, 855, 983, 1305
BstAFI CTTAAG 1 cut(s) 1367
BstAPI GCANNNNNTGC 2 cut(s) 47, 227
BstC8I GCNNGC 3 cut(s) 294, 495, 844
BstDEI CTNAG 3 cut(s) 198, 231, 1023
BstF5I GGATG 4 cut(s) 213, 636, 1081, 1178
BstFNI CGCG 1 cut(s) 918
BstKTI GATC 5 cut(s) 484, 507, 557, 981, 1123
BstMAI GTCTC 4 cut(s) 687, 712, 728, 1111
BstMBI GATC 5 cut(s) 481, 504, 554, 978, 1120
BstMWI GCNNNNNNNGC 6 cut(s) 47, 218, 227, 521, 1019, 1091
BstNI CCWGG 2 cut(s) 218, 540
BstNSI RCATGY 1 cut(s) 1085
BstSCI CCNGG 2 cut(s) 216, 538
BstUI CGCG 1 cut(s) 918
BstV1I GCAGC 1 cut(s) 1320
BstV2I GAAGAC 2 cut(s) 287, 384
BstX2I RGATCY 2 cut(s) 504, 554
BstYI RGATCY 2 cut(s) 504, 554
BsuRI GGCC 1 cut(s) 547
BtgZI GCGATG 1 cut(s) 933
BtsCI GGATG 4 cut(s) 213, 636, 1081, 1178
Cac8I GCNNGC 3 cut(s) 294, 495, 844
CciI TCATGA 1 cut(s) 1048
Cfr13I GGNCC 3 cut(s) 432, 536, 602
Csp6I GTAC 1 cut(s) 657
CviAII CATG 9 cut(s) 95, 289, 304, 619, 838, 1049, 1082, 1160, 1374
CviQI GTAC 1 cut(s) 657
DdeI CTNAG 3 cut(s) 198, 231, 1023
DpnI GATC 5 cut(s) 483, 506, 556, 980, 1122
DpnII GATC 5 cut(s) 481, 504, 554, 978, 1120
DrdI GACNNNNNNGTC 1 cut(s) 1095
DseDI GACNNNNNNGTC 1 cut(s) 1095
Eam1104I CTCTTC 5 cut(s) 152, 176, 855, 983, 1305
EarI CTCTTC 5 cut(s) 152, 176, 855, 983, 1305
Ecl136II GAGCTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 728
Eco47I GGWCC 3 cut(s) 432, 536, 602
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 771
EcoICRI GAGCTC 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 216, 538
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 687
FaeI CATG 9 cut(s) 98, 292, 307, 622, 841, 1052, 1085, 1163, 1377
FatI CATG 9 cut(s) 94, 288, 303, 618, 837, 1048, 1081, 1159, 1373
FauI CCCGC 1 cut(s) 312
FbaI TGATCA 1 cut(s) 1120
FblI GTMKAC 1 cut(s) 1041
Fnu4HI GCNGC 1 cut(s) 1334
FokI GGATG 4 cut(s) 200, 643, 1088, 1185
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 1 cut(s) 1334
FspBI CTAG 9 cut(s) 146, 243, 468, 494, 564, 795, 857, 926, 1304
GluI GCNGC 1 cut(s) 1334
GsaI CCCAGC 1 cut(s) 41
HaeIII GGCC 1 cut(s) 547
Hin1II CATG 9 cut(s) 98, 292, 307, 622, 841, 1052, 1085, 1163, 1377
HincII GTYRAC 2 cut(s) 325, 891
HindII GTYRAC 2 cut(s) 325, 891
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 8 cut(s) 262, 283, 590, 742, 815, 983, 1180, 1215
HphI GGTGA 5 cut(s) 44, 289, 364, 383, 404
Hpy166II GTNNAC 6 cut(s) 325, 395, 401, 435, 891, 1042
Hpy188I TCNGA 2 cut(s) 667, 820
Hpy188III TCNNGA 4 cut(s) 85, 518, 926, 1049
Hpy8I GTNNAC 6 cut(s) 325, 395, 401, 435, 891, 1042
Hpy99I CGWCG 1 cut(s) 649
HpyAV CCTTC 6 cut(s) 245, 413, 665, 709, 746, 1205
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 6 cut(s) 397, 644, 662, 680, 765, 1164
HpyCH4V TGCA 6 cut(s) 167, 292, 524, 1085, 1176, 1273
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 218, 227, 521, 1019, 1091
HpyF3I CTNAG 3 cut(s) 198, 231, 1023
HpySE526I ACGT 6 cut(s) 397, 644, 662, 680, 765, 1164
Hsp92II CATG 9 cut(s) 98, 292, 307, 622, 841, 1052, 1085, 1163, 1377
Ksp22I TGATCA 1 cut(s) 1120
Kzo9I GATC 5 cut(s) 481, 504, 554, 978, 1120
LguI GCTCTTC 1 cut(s) 855
LmnI GCTCC 2 cut(s) 39, 103
Lsp1109I GCAGC 1 cut(s) 1320
LweI GCATC 6 cut(s) 178, 306, 511, 624, 910, 1163
MaeI CTAG 9 cut(s) 146, 243, 468, 494, 564, 795, 857, 926, 1304
MaeII ACGT 6 cut(s) 397, 644, 662, 680, 765, 1164
MaeIII GTNAC 3 cut(s) 13, 103, 352
MalI GATC 5 cut(s) 483, 506, 556, 980, 1122
MboI GATC 5 cut(s) 481, 504, 554, 978, 1120
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 2 cut(s) 504, 554
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 6 cut(s) 63, 204, 606, 866, 995, 1227
MlyI GAGTC 4 cut(s) 277, 736, 992, 1224
MmeI TCCRAC 2 cut(s) 770, 1306
MseI TTAA 4 cut(s) 207, 1167, 1196, 1368
MspCI CTTAAG 1 cut(s) 1367
MspR9I CCNGG 2 cut(s) 218, 540
MunI CAATTG 1 cut(s) 866
MvaI CCWGG 2 cut(s) 218, 540
MvnI CGCG 1 cut(s) 918
MwoI GCNNNNNNNGC 6 cut(s) 47, 218, 227, 521, 1019, 1091
NdeII GATC 5 cut(s) 481, 504, 554, 978, 1120
NheI GCTAGC 1 cut(s) 493
NlaIII CATG 9 cut(s) 98, 292, 307, 622, 841, 1052, 1085, 1163, 1377
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 3 cut(s) 573, 1039, 1362
NmuCI GTSAC 2 cut(s) 103, 352
NspI RCATGY 1 cut(s) 1085
PagI TCATGA 1 cut(s) 1048
PciSI GCTCTTC 1 cut(s) 855
PfeI GAWTC 4 cut(s) 262, 590, 815, 1180
PkrI GCNGC 1 cut(s) 1335
PleI GAGTC 4 cut(s) 277, 736, 991, 1223
PpsI GAGTC 4 cut(s) 277, 736, 991, 1223
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 216, 538
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 2 cut(s) 216, 538
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 432, 536, 602
PsuI RGATCY 2 cut(s) 504, 554
RsaI GTAC 1 cut(s) 658
RsaNI GTAC 1 cut(s) 657
SacI GAGCTC 1 cut(s) 175
SapI GCTCTTC 1 cut(s) 855
SaqAI TTAA 4 cut(s) 207, 1167, 1196, 1368
SatI GCNGC 1 cut(s) 1334
Sau3AI GATC 5 cut(s) 481, 504, 554, 978, 1120
Sau96I GGNCC 3 cut(s) 432, 536, 602
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 4 cut(s) 277, 736, 992, 1224
ScrFI CCNGG 2 cut(s) 218, 540
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 6 cut(s) 178, 306, 511, 624, 910, 1163
SinI GGWCC 3 cut(s) 432, 536, 602
SmlI CTYRAG 4 cut(s) 192, 268, 1243, 1367
SmoI CTYRAG 4 cut(s) 192, 268, 1243, 1367
Sse9I AATT 6 cut(s) 63, 204, 606, 866, 995, 1227
SsiI CCGC 1 cut(s) 319
SspMI CTAG 9 cut(s) 146, 243, 468, 494, 564, 795, 857, 926, 1304
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 216, 538
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 6 cut(s) 400, 647, 665, 683, 768, 1167
TaqI TCGA 4 cut(s) 417, 740, 981, 986
TaqII GACCGA 1 cut(s) 1056
TasI AATT 6 cut(s) 63, 204, 606, 866, 995, 1227
TatI WGTACW 1 cut(s) 656
TfiI GAWTC 4 cut(s) 262, 590, 815, 1180
Tru1I TTAA 4 cut(s) 207, 1167, 1196, 1368
Tru9I TTAA 4 cut(s) 207, 1167, 1196, 1368
TseFI GTSAC 2 cut(s) 103, 352
TseI GCWGC 1 cut(s) 1333
Tsp45I GTSAC 2 cut(s) 103, 352
TspDTI ATGAA 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 1367
VpaK11BI GGWCC 3 cut(s) 432, 536, 602
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 1085
XmiI GTMKAC 1 cut(s) 1041
XspI CTAG 9 cut(s) 146, 243, 468, 494, 564, 795, 857, 926, 1304
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.