Rh4CG052300

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000949
Physical Location & Seq
Reverse (-)
10357924 .. 10358511
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG052300.1

Sequence Viewer

Length: 588 bp
ATGGAGGTTAACCAAAGCCAGGAGGCCCCTAAAGCTCCGAGGACCCTGATGCCCTCTCATTTCATGGTTGGCACTATCCCTGTTTACCTGCCCACCGTAGAGACTGTGATGGTAACTTCCACTAAGGGAAGTGATGAAGACAATTACAAGGATAAGCGTGAAGCTCGTCCTTCACATGGAAGCTCACAGAGGTCAACCATGCCTTCACAACAAGCAGCATCAGGGGAGAGCCAAGACCTGGACCCAAAGAAACTAGCTGATGAGATAGTGAAGGCCCTCGATGCACCACCAAAAGCTCCAGGGAGGCCAAAGGGAGCCAAGAACAAAGAGTATCCGGTTTATGGAGGGGATTCTTACCCCGGACCAGTTACTCGCAATATGGCTAAAAAGATGACGCCTGGTACATCATCAACAAGTACCACGTCAGAAGAGGGATATTTGTCACTGAAGGACAATCCTTTATATGTGTCCTGGAAGGGTAATCCTTTATATGAGGCATCAATGCCTAAAGAGGGCTCTACACAAGCGTTAATGCCTCAGTCACATGTTGCAACATCTAGTGAAAGTGGTGATTCTGGCGATGCACAT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

196

Amino Acids

21.03

Weight (kDa)

6.1

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 2 cut(s) 403, 418
AfiI CCNNNNNNNGG 5 cut(s) 19, 176, 238, 341, 512
AflIII ACRYGT 1 cut(s) 544
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 5 cut(s) 18, 237, 298, 397, 470
AluBI AGCT 5 cut(s) 35, 164, 183, 257, 296
AluI AGCT 5 cut(s) 35, 164, 183, 257, 296
Alw26I GTCTC 1 cut(s) 95
AoxI GGCC 3 cut(s) 24, 273, 305
ApeKI GCWGC 1 cut(s) 215
AspS9I GGNCC 5 cut(s) 25, 42, 241, 274, 362
AsuC2I CCSGG 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 581
AvaII GGWCC 3 cut(s) 42, 241, 362
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 227
BccI CCATC 1 cut(s) 103
BciT130I CCWGG 5 cut(s) 20, 239, 300, 399, 472
BciVI GTATCC 1 cut(s) 342
BcnI CCSGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 2 cut(s) 254, 558
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 342
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 6 cut(s) 20, 239, 300, 360, 399, 472
Bme18I GGWCC 3 cut(s) 42, 241, 362
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 5 cut(s) 25, 42, 241, 274, 362
BmiI GGNNCC 4 cut(s) 27, 44, 243, 316
BmrFI CCNGG 6 cut(s) 20, 239, 300, 360, 399, 472
BmsI GCATC 5 cut(s) 39, 227, 271, 506, 571
BpiI GAAGAC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 395
BsaJI CCNNGG 3 cut(s) 38, 299, 358
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 176, 238, 341, 512
Bse1I ACTGG 1 cut(s) 365
BseBI CCWGG 5 cut(s) 20, 239, 300, 399, 472
BseDI CCNNGG 3 cut(s) 38, 299, 358
BseLI CCNNNNNNNGG 5 cut(s) 19, 176, 238, 341, 512
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 365
BseXI GCAGC 1 cut(s) 227
BshFI GGCC 3 cut(s) 26, 275, 307
BsiSI CCGG 2 cut(s) 335, 360
BslI CCNNNNNNNGG 5 cut(s) 19, 176, 238, 341, 512
BsmAI GTCTC 1 cut(s) 95
BsnI GGCC 3 cut(s) 26, 275, 307
Bsp1286I GDGCHC 1 cut(s) 518
BspANI GGCC 3 cut(s) 26, 275, 307
BspCNI CTCAG 1 cut(s) 550
BspLI GGNNCC 4 cut(s) 27, 44, 243, 316
BspMI ACCTGC 1 cut(s) 96
BsrI ACTGG 1 cut(s) 365
BssECI CCNNGG 3 cut(s) 38, 299, 358
BssNI GRCGYC 1 cut(s) 395
Bst2UI CCWGG 5 cut(s) 20, 239, 300, 399, 472
Bst4CI ACNGT 2 cut(s) 97, 106
Bst6I CTCTTC 1 cut(s) 423
BstACI GRCGYC 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 123, 537
BstMAI GTCTC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 32, 281
BstNI CCWGG 5 cut(s) 20, 239, 300, 399, 472
BstNSI RCATGY 1 cut(s) 548
BstSCI CCNGG 6 cut(s) 18, 237, 298, 358, 397, 470
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 144
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 3 cut(s) 26, 275, 307
BtrI CACGTC 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 443
BveI ACCTGC 1 cut(s) 96
Cfr13I GGNCC 5 cut(s) 25, 42, 241, 274, 362
CseI GACGC 1 cut(s) 403
Csp6I GTAC 2 cut(s) 402, 417
CviAII CATG 4 cut(s) 64, 176, 199, 545
CviQI GTAC 2 cut(s) 402, 417
DdeI CTNAG 2 cut(s) 123, 537
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 518
Eco47I GGWCC 3 cut(s) 42, 241, 362
Eco57I CTGAAG 1 cut(s) 467
EcoO109I RGGNCCY 3 cut(s) 25, 42, 274
EcoRII CCWGG 5 cut(s) 18, 237, 298, 397, 470
EcoT38I GRGCYC 1 cut(s) 518
FaeI CATG 4 cut(s) 67, 179, 202, 548
FatI CATG 4 cut(s) 63, 175, 198, 544
Fnu4HI GCNGC 1 cut(s) 216
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 254, 558
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 3 cut(s) 26, 275, 307
HapII CCGG 2 cut(s) 335, 360
HgaI GACGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 4 cut(s) 67, 179, 202, 548
HincII GTYRAC 2 cut(s) 10, 195
HindII GTYRAC 2 cut(s) 10, 195
HinfI GANTC 2 cut(s) 350, 572
HpaI GTTAAC 1 cut(s) 10
HpaII CCGG 2 cut(s) 335, 360
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 3 cut(s) 10, 85, 195
Hpy188I TCNGA 2 cut(s) 39, 427
Hpy8I GTNNAC 3 cut(s) 10, 85, 195
HpyAV CCTTC 5 cut(s) 180, 213, 265, 442, 469
HpyCH4III ACNGT 2 cut(s) 97, 106
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 3 cut(s) 284, 551, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 281
HpyF3I CTNAG 2 cut(s) 123, 537
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 4 cut(s) 67, 179, 202, 548
KspAI GTTAAC 1 cut(s) 10
LmnI GCTCC 3 cut(s) 40, 301, 314
Lsp1109I GCAGC 1 cut(s) 227
LweI GCATC 5 cut(s) 39, 227, 271, 506, 571
MaeI CTAG 2 cut(s) 254, 558
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 4 cut(s) 112, 367, 441, 540
MboII GAAGA 2 cut(s) 149, 440
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 1 cut(s) 142
MseI TTAA 2 cut(s) 9, 530
MspI CCGG 2 cut(s) 335, 360
MspR9I CCNGG 6 cut(s) 20, 239, 300, 360, 399, 472
MvaI CCWGG 5 cut(s) 20, 239, 300, 399, 472
MwoI GCNNNNNNNGC 2 cut(s) 32, 281
NciI CCSGG 1 cut(s) 360
NlaIII CATG 4 cut(s) 67, 179, 202, 548
NlaIV GGNNCC 4 cut(s) 27, 44, 243, 316
NmuCI GTSAC 2 cut(s) 441, 540
NspI RCATGY 1 cut(s) 548
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 2 cut(s) 350, 572
PflMI CCANNNNNTGG 1 cut(s) 238
PfoI TCCNGGA 1 cut(s) 470
PkrI GCNGC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 42
PscI ACATGT 1 cut(s) 544
Psp5II RGGWCCY 1 cut(s) 42
Psp6I CCWGG 5 cut(s) 18, 237, 298, 397, 470
PspGI CCWGG 5 cut(s) 18, 237, 298, 397, 470
PspN4I GGNNCC 4 cut(s) 27, 44, 243, 316
PspPI GGNCC 5 cut(s) 25, 42, 241, 274, 362
PspPPI RGGWCCY 1 cut(s) 42
RsaI GTAC 2 cut(s) 403, 418
RsaNI GTAC 2 cut(s) 402, 417
SaqAI TTAA 2 cut(s) 9, 530
SatI GCNGC 1 cut(s) 216
Sau96I GGNCC 5 cut(s) 25, 42, 241, 274, 362
ScrFI CCNGG 6 cut(s) 20, 239, 300, 360, 399, 472
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 5 cut(s) 39, 227, 271, 506, 571
SinI GGWCC 3 cut(s) 42, 241, 362
Sse9I AATT 1 cut(s) 142
SspMI CTAG 2 cut(s) 254, 558
StyD4I CCNGG 6 cut(s) 18, 237, 298, 358, 397, 470
TaaI ACNGT 2 cut(s) 97, 106
TaiI ACGT 1 cut(s) 425
TaqI TCGA 1 cut(s) 279
TasI AATT 1 cut(s) 142
TfiI GAWTC 2 cut(s) 350, 572
Tru1I TTAA 2 cut(s) 9, 530
Tru9I TTAA 2 cut(s) 9, 530
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 2 cut(s) 441, 540
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 441, 540
TspDTI ATGAA 2 cut(s) 52, 150
TspRI CASTG 1 cut(s) 450
Van91I CCANNNNNTGG 1 cut(s) 238
VpaK11BI GGWCC 3 cut(s) 42, 241, 362
XceI RCATGY 1 cut(s) 548
XspI CTAG 2 cut(s) 254, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.