Rroxscaffold_5G00364750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000949
Physical Location & Seq
Forward (+)
45925396 .. 45926166
771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364750.1

Sequence Viewer

Length: 444 bp
ATGCCGGGATCTGTTGGGACAAAGAAAATTTCCAAGGCTAATTATGGCAAAGTGAAAGGTGGAAAGAATCCCAATGCAAAGGGGTTGGACGTGCTAATCCCTACCCACGTGGCAATAATGCACTACATGGCAAGGGACATGGGGGTCATGATATGGGTCGTGGAGGTCCTCCCAATGTATGGCACAGAGATGGTGGTGCTGGCCCTAGTGGTCATGGAAACAAGGCAACTTGGCAAGGCCGGGAAGGTGGCTTCCGGCGACGTATTAGCCGCTTCGATACCGTGGCGTAGCTTGGACATCGGGCTACGGGATGCATGTCTCGGTTGGCCCCCCAAGAGGGTGTTACTTATGCTTGGTGACATAGAAAATAGCCTTGCTAGTAGAGGTATCTACAAGTCCCCAAAGTCCCCAAGTAAGAGGAGCTTCTTGGTTGGGGAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

15.85

Weight (kDa)

9.85

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 442
AasI GACNNNNNNGTC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 179
AciI CCGC 1 cut(s) 270
AclWI GGATC 1 cut(s) 16
AcsI RAATTY 1 cut(s) 27
AcvI CACGTG 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 179, 336, 337
AjiI CACGTC 1 cut(s) 91
AluBI AGCT 2 cut(s) 291, 423
AluI AGCT 2 cut(s) 291, 423
Alw26I GTCTC 1 cut(s) 323
AlwI GGATC 1 cut(s) 16
AoxI GGCC 3 cut(s) 201, 237, 326
ApoI RAATTY 1 cut(s) 27
AspS9I GGNCC 3 cut(s) 166, 202, 327
AsuC2I CCSGG 2 cut(s) 6, 241
AsuHPI GGTGA 1 cut(s) 368
AvaII GGWCC 1 cut(s) 166
BbrPI CACGTG 1 cut(s) 109
BccI CCATC 1 cut(s) 184
BcnI CCSGG 2 cut(s) 6, 241
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 2 cut(s) 206, 378
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 6, 241
Bme18I GGWCC 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 3 cut(s) 166, 202, 327
BmiI GGNNCC 1 cut(s) 329
BmrFI CCNGG 2 cut(s) 6, 241
BmsI GCATC 1 cut(s) 301
BpuMI CCSGG 2 cut(s) 6, 241
BsaAI YACGTR 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 33, 281
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 336, 337
BseDI CCNNGG 2 cut(s) 33, 281
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 3 cut(s) 179, 336, 337
BseRI GAGGAG 1 cut(s) 433
BshFI GGCC 3 cut(s) 203, 239, 328
BsiSI CCGG 3 cut(s) 5, 240, 255
BslFI GGGAC 4 cut(s) 31, 149, 382, 391
BslI CCNNNNNNNGG 3 cut(s) 179, 336, 337
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 4 cut(s) 31, 149, 382, 391
BsnI GGCC 3 cut(s) 203, 239, 328
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 1 cut(s) 270
BspANI GGCC 3 cut(s) 203, 239, 328
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 329
BspPI GGATC 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 33, 281
BssMI GATC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 33
Bst4CI ACNGT 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 109
BstC8I GCNNGC 1 cut(s) 201
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 8
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 2 cut(s) 4, 239
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 3 cut(s) 203, 239, 328
BtgI CCRYGG 1 cut(s) 281
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 147
Cfr13I GGNCC 3 cut(s) 166, 202, 327
CviAII CATG 5 cut(s) 127, 139, 148, 214, 315
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 166
Eco72I CACGTG 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 5 cut(s) 130, 142, 151, 217, 318
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 4 cut(s) 31, 149, 382, 391
FatI CATG 5 cut(s) 126, 138, 147, 213, 314
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 2 cut(s) 206, 378
GluI GCNGC 1 cut(s) 270
HaeIII GGCC 3 cut(s) 203, 239, 328
HapII CCGG 3 cut(s) 5, 240, 255
Hin1II CATG 5 cut(s) 130, 142, 151, 217, 318
HinfI GANTC 1 cut(s) 67
HpaII CCGG 3 cut(s) 5, 240, 255
HphI GGTGA 1 cut(s) 368
Hpy188III TCNNGA 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4IV ACGT 3 cut(s) 90, 108, 261
HpyCH4V TGCA 3 cut(s) 77, 121, 314
HpySE526I ACGT 3 cut(s) 90, 108, 261
Hsp92II CATG 5 cut(s) 130, 142, 151, 217, 318
Kzo9I GATC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 420
LpnPI CCDG 4 cut(s) 18, 185, 253, 268
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 206, 378
MaeII ACGT 3 cut(s) 90, 108, 261
MaeIII GTNAC 2 cut(s) 342, 356
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MflI RGATCY 1 cut(s) 8
MluCI AATT 2 cut(s) 27, 40
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 5 cut(s) 157, 179, 330, 377, 411
Mph1103I ATGCAT 1 cut(s) 316
MslI CAYNNNNRTG 1 cut(s) 188
MspI CCGG 3 cut(s) 5, 240, 255
MspR9I CCNGG 2 cut(s) 6, 241
NciI CCSGG 2 cut(s) 6, 241
NdeII GATC 1 cut(s) 8
NlaIII CATG 5 cut(s) 130, 142, 151, 217, 318
NlaIV GGNNCC 1 cut(s) 329
NmuCI GTSAC 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 1 cut(s) 318
PagI TCATGA 1 cut(s) 147
PfeI GAWTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 179
PkrI GCNGC 1 cut(s) 271
PmaCI CACGTG 1 cut(s) 109
PmlI CACGTG 1 cut(s) 109
Ppu21I YACGTR 1 cut(s) 109
PpuMI RGGWCCY 1 cut(s) 166
PsiI TTATAA 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 166
PspCI CACGTG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 329
PspPI GGNCC 3 cut(s) 166, 202, 327
PspPPI RGGWCCY 1 cut(s) 166
PsuI RGATCY 1 cut(s) 8
RseI CAYNNNNRTG 1 cut(s) 188
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 8
Sau96I GGNCC 3 cut(s) 166, 202, 327
ScrFI CCNGG 2 cut(s) 6, 241
SetI ASST 9 cut(s) 61, 93, 111, 168, 249, 264, 293, 388, 425
SfaNI GCATC 1 cut(s) 301
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 188
Sse9I AATT 2 cut(s) 27, 40
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 206, 378
StyD4I CCNGG 2 cut(s) 4, 239
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 282
TaiI ACGT 3 cut(s) 93, 111, 264
TaqI TCGA 1 cut(s) 275
TasI AATT 2 cut(s) 27, 40
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 1 cut(s) 67
TseFI GTSAC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 356
Van91I CCANNNNNTGG 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 318
XspI CTAG 2 cut(s) 206, 378
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.