Rh4CG032100

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000949
Physical Location & Seq
Reverse (-)
6362895 .. 6363619
725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG032100.1

Sequence Viewer

Length: 540 bp
ATGGTTGGCACTGTCCCAGTCTATCTACCTAACACTGAGAGTGTGATGGTGACCTCTATTGAGGAGAAAGATGAAGACGAAGCTTCAGTGGCTCAAGAGTCCACTACCTCTTCACAAAGAGTTATCATACAGGAAGGTCAAAACATCGACCCAAAGCAAATAGCTGAAGGGGTAATCAAGGCCCTCGATGCACCATCAAGGCGTGCAGGGAGGCCAAAAGGGGCTAGAAACAAAGAGTATCCGGTTTTCAAAGATGACTTTTACTCAGGGCCAGTCACTCGCAACAAGGCTCACGAAATGACGCCTAGAAATGCTTCTTCAGTGGGCACTGCATCAGAAGATGAGGGACAAACGTGGAAAGATAATCCACTCTATGAGACATCTGTAAAAGAAAGAGTCTCCCAGCTGCAAACTGACGGCTCATCACAGGGTTCAGAAAGCGTTGAAAGCAGAGAACCCGATGGTGCACAAGTGCTGGTAGCTAACCCGCAAAGCCCTGAAGAACAGATGCAAGAGTTGCAGAGAAGAACGGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 Retrotrans_gag rve

Protein Analysis

179

Amino Acids

19.67

Weight (kDa)

4.66

Isoelectric Point (pI)

66.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 488
AcuI CTGAAG 4 cut(s) 69, 186, 303, 519
AcyI GRCGYC 1 cut(s) 302
AgsI TTSAA 2 cut(s) 250, 446
AluBI AGCT 4 cut(s) 83, 164, 406, 482
AluI AGCT 4 cut(s) 83, 164, 406, 482
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 2 cut(s) 371, 403
Alw44I GTGCAC 1 cut(s) 465
AoxI GGCC 3 cut(s) 180, 212, 269
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 1 cut(s) 406
Asp700I GAANNNNTTC 1 cut(s) 313
AspS9I GGNCC 2 cut(s) 181, 269
AsuHPI GGTGA 1 cut(s) 61
BaeGI GKGCMC 2 cut(s) 329, 469
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 1 cut(s) 393
BccI CCATC 3 cut(s) 40, 202, 455
BceAI ACGGC 1 cut(s) 433
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 2 cut(s) 371, 403
BfaI CTAG 2 cut(s) 225, 306
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 1 cut(s) 407
BlsI GCNGC 1 cut(s) 408
BmgT120I GGNCC 2 cut(s) 181, 269
BmrI ACTGGG 1 cut(s) 11
BmsI GCATC 3 cut(s) 178, 341, 498
BmuI ACTGGG 1 cut(s) 11
BpiI GAAGAC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 302
BsaWI WCCGGW 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 17, 272
BseMII CTCAG 2 cut(s) 27, 279
BseNI ACTGG 2 cut(s) 17, 272
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 2 cut(s) 329, 469
BseXI GCAGC 1 cut(s) 393
BseYI CCCAGC 1 cut(s) 402
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 3 cut(s) 182, 214, 271
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 360
BsmAI GTCTC 2 cut(s) 371, 403
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 3 cut(s) 182, 214, 271
Bsp1286I GDGCHC 2 cut(s) 329, 469
BspACI CCGC 1 cut(s) 488
BspANI GGCC 3 cut(s) 182, 214, 271
BspCNI CTCAG 2 cut(s) 28, 278
BsrI ACTGG 2 cut(s) 17, 272
BssNI GRCGYC 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 2 cut(s) 36, 265
BstEII GGTNACC 1 cut(s) 49
BstMAI GTCTC 2 cut(s) 371, 403
BstMWI GCNNNNNNNGC 4 cut(s) 89, 188, 447, 517
BstPI GGTNACC 1 cut(s) 49
BstSLI GKGCMC 2 cut(s) 329, 469
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 1 cut(s) 81
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 3 cut(s) 182, 214, 271
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 5 cut(s) 9, 33, 93, 327, 327
Cac8I GCNNGC 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 181, 269
CseI GACGC 1 cut(s) 310
CviAII CATG 1 cut(s) 537
DdeI CTNAG 2 cut(s) 36, 265
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco57I CTGAAG 4 cut(s) 69, 186, 303, 519
Eco91I GGTNACC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 181
EcoO65I GGTNACC 1 cut(s) 49
FaeI CATG 1 cut(s) 540
FaiI YATR 3 cut(s) 128, 375, 538
FaqI GGGAC 1 cut(s) 360
FatI CATG 1 cut(s) 536
FauI CCCGC 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 407
Fsp4HI GCNGC 1 cut(s) 407
FspBI CTAG 2 cut(s) 225, 306
GluI GCNGC 1 cut(s) 407
GsaI CCCAGC 1 cut(s) 406
HaeIII GGCC 3 cut(s) 182, 214, 271
HapII CCGG 1 cut(s) 242
HgaI GACGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 1 cut(s) 540
HindIII AAGCTT 1 cut(s) 81
HinfI GANTC 2 cut(s) 98, 396
HpaII CCGG 1 cut(s) 242
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 102, 467
Hpy188I TCNGA 2 cut(s) 337, 436
Hpy188III TCNNGA 2 cut(s) 95, 293
Hpy8I GTNNAC 2 cut(s) 102, 467
HpyAV CCTTC 2 cut(s) 128, 161
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 7 cut(s) 191, 206, 332, 409, 467, 511, 520
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 188, 447, 517
HpyF3I CTNAG 2 cut(s) 36, 265
HpySE526I ACGT 1 cut(s) 353
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 1 cut(s) 540
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 3 cut(s) 178, 341, 498
MaeI CTAG 2 cut(s) 225, 306
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 2 cut(s) 49, 274
MboII GAAGA 6 cut(s) 86, 102, 309, 350, 512, 537
MhlI GDGCHC 2 cut(s) 329, 469
MlyI GAGTC 2 cut(s) 107, 405
MnlI CCTC 6 cut(s) 55, 64, 118, 194, 204, 337
MroXI GAANNNNTTC 1 cut(s) 313
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 1 cut(s) 242
MwoI GCNNNNNNNGC 4 cut(s) 89, 188, 447, 517
NlaIII CATG 1 cut(s) 540
NmuCI GTSAC 2 cut(s) 49, 274
PdmI GAANNNNTTC 1 cut(s) 313
PkrI GCNGC 1 cut(s) 408
PleI GAGTC 2 cut(s) 106, 404
PpsI GAGTC 2 cut(s) 106, 404
PspEI GGTNACC 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 402
PspPI GGNCC 2 cut(s) 181, 269
PvuII CAGCTG 1 cut(s) 406
SatI GCNGC 1 cut(s) 407
Sau96I GGNCC 2 cut(s) 181, 269
SchI GAGTC 2 cut(s) 107, 405
SduI GDGCHC 2 cut(s) 329, 469
SetI ASST 9 cut(s) 31, 56, 85, 110, 139, 166, 356, 408, 484
SfaNI GCATC 3 cut(s) 178, 341, 498
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
SsiI CCGC 1 cut(s) 488
SspMI CTAG 2 cut(s) 225, 306
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 147, 186
TscAI CASTG 5 cut(s) 16, 40, 93, 327, 334
TseFI GTSAC 2 cut(s) 49, 274
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 49, 274
TspDTI ATGAA 1 cut(s) 87
TspRI CASTG 5 cut(s) 16, 40, 93, 327, 334
VneI GTGCAC 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 313
XspI CTAG 2 cut(s) 225, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.