FvH4_5g04540

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000675
Physical Location & Seq
Reverse (-)
2673051 .. 2674348
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04540.t1

Sequence Viewer

Length: 1125 bp
ATGGCTGCCTTGAACATTTTCATCACCTTCTTCTTCTTCTTGTTCTTCTTCCTTCCTCATATAACAGCTGCCACATGCCCTCGATCGACATGCACTTCCCACAGCCCATCCATTCGATTCCCTTTCCGACTAGCAAATCAACAAAAACTCTCAAACTCTGGGTATTTAGGGTTTGATCTCTCACGCAATAACCAAAAACAAACCATCCTCACTCTCCCATCTTCGGGGGACTTCGTTGTCCAAAACATCAACTACAAAGAACAAATTGTAACGATCAATAACCCCGAAAACTGCCTCCCAAAACTGTTCCTCGATGGTAACTACTACTTCAACCTTACAGATTCACCCTTCACATTCTTAACTGACATCAGAAACTACACCTTCCTCAACTGTTCGACTCAAGATGGAACGTGGGCTGCATCCATCGCTTGCCTCAGCGATGACTACTATAAGGTCCTTCTTGTGCCTACACCACGTTGGCCAACGTCATGGTTTCCTTGCTCTATCATCTCCACGGCTTTGGTTCCAATATATTCACTTCAGTGGAGAGATCTTGACGACGTCCTTGGTATTCATCTGTCATGGAACGAACCTGATTGTCGTTCTTGTGAAGCAGGAGGTCTGCGTTGTGAGTTTCAGAATGCTACAAGCTCGCAAGTTCAGTGCTCCGTTTCTTCACACACTGGTCTTTCAGGATCAGCGAAGTATGGAATTATCATAGGCGTTGGAATACCAGGGATATTGTGCACTCTTGGGATTGGTCTTTATGTTTGTGACAGGGTAGGGAGAGTGCGAGATGGACTACTCCACCAGCGGCAGTCCATCACAGAATTATCCACCGCAGTTGATCAACAAGTAGAACCAGCTCCCCTGGTTATAGTTGGGCTTGATGGTCGGACAATCGAGTCGTATCCCAATTTCCAACTAGATGAGAGCTTGGAGTTGCCCGAGCCTGATGATAAGACATGTTCCATATGTTTGTGTGAGTACCAACCCAAGGAAACAATACGAATCATACCAGACTGCAACCATTATTTTCATGCTAGTTGCATATACGAGTGGCTTAGAAAGAATCCGACGTGCCCTCTTTGCCGAAATCCGCCGGAGGGCTTGCTGAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

375

Amino Acids

42.01

Weight (kDa)

5.39

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 26 - 93 6.7e-13 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 322 - 365 1.1e-13 Ring finger domain
zf-RING_11 PF17123 323 - 351 2.5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 323 - 364 2.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 327 - 365 2.5e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1123
AasI GACNNNNNNGTC 2 cut(s) 236, 904
AatII GACGTC 1 cut(s) 564
AciI CCGC 3 cut(s) 814, 840, 1100
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 1 cut(s) 479
AcuI CTGAAG 1 cut(s) 524
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 1 cut(s) 989
AfiI CCNNNNNNNGG 4 cut(s) 223, 224, 997, 1106
AflIII ACRYGT 1 cut(s) 965
AgsI TTSAA 2 cut(s) 13, 331
AjiI CACGTC 1 cut(s) 1080
AjnI CCWGG 2 cut(s) 733, 870
AleI CACNNNNGTG 1 cut(s) 541
AluBI AGCT 4 cut(s) 68, 651, 866, 936
AluI AGCT 4 cut(s) 68, 651, 866, 936
Alw21I GWGCWC 2 cut(s) 668, 749
Alw44I GTGCAC 1 cut(s) 745
AlwI GGATC 1 cut(s) 703
Ama87I CYCGRG 1 cut(s) 947
AoxI GGCC 1 cut(s) 479
ApaLI GTGCAC 1 cut(s) 745
ApeKI GCWGC 3 cut(s) 5, 68, 416
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 454
AsuHPI GGTGA 2 cut(s) 16, 336
AvaI CYCGRG 1 cut(s) 947
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 2 cut(s) 749, 1085
BalI TGGCCA 1 cut(s) 481
Bbv12I GWGCWC 2 cut(s) 668, 749
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 2 cut(s) 55, 403
BccI CCATC 9 cut(s) 115, 212, 226, 308, 398, 431, 791, 830, 884
BceAI ACGGC 1 cut(s) 531
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 2 cut(s) 735, 872
BciVI GTATCC 1 cut(s) 921
BclI TGATCA 1 cut(s) 847
BfaI CTAG 3 cut(s) 131, 926, 1044
BfuI GTATCC 1 cut(s) 921
BglII AGATCT 1 cut(s) 550
BisI GCNGC 4 cut(s) 6, 69, 417, 815
BlsI GCNGC 4 cut(s) 7, 70, 418, 816
Bme1390I CCNGG 2 cut(s) 735, 872
Bme18I GGWCC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 947
BmgBI CACGTC 1 cut(s) 1080
BmgT120I GGNCC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 2 cut(s) 735, 872
BmsI GCATC 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 434
BpuEI CTTGAG 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 5 cut(s) 513, 565, 734, 870, 996
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 224, 997, 1106
Bse1I ACTGG 1 cut(s) 688
BseBI CCWGG 2 cut(s) 735, 872
BseDI CCNNGG 5 cut(s) 513, 565, 734, 870, 996
BseGI GGATG 3 cut(s) 107, 204, 419
BseLI CCNNNNNNNGG 4 cut(s) 223, 224, 997, 1106
BseMII CTCAG 1 cut(s) 448
BseNI ACTGG 1 cut(s) 688
BseSI GKGCMC 2 cut(s) 749, 1085
BseXI GCAGC 2 cut(s) 55, 403
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 481
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 668, 749
BsiHKCI CYCGRG 1 cut(s) 947
BsiSI CCGG 1 cut(s) 1103
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 4 cut(s) 223, 224, 997, 1106
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 1 cut(s) 481
BsoBI CYCGRG 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 668, 749, 1085
Bsp143I GATC 6 cut(s) 83, 175, 273, 550, 695, 847
BspACI CCGC 3 cut(s) 814, 840, 1100
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 1 cut(s) 447
BspLI GGNNCC 1 cut(s) 525
BspPI GGATC 1 cut(s) 703
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 5 cut(s) 513, 565, 734, 870, 996
BssMI GATC 6 cut(s) 83, 175, 273, 550, 695, 847
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 2 cut(s) 565, 996
Bst2UI CCWGG 2 cut(s) 735, 872
Bst4CI ACNGT 2 cut(s) 306, 392
BstACI GRCGYC 1 cut(s) 561
BstC8I GCNNGC 3 cut(s) 430, 653, 1112
BstDEI CTNAG 2 cut(s) 434, 1064
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 107, 204, 419
BstKTI GATC 6 cut(s) 86, 178, 276, 553, 698, 850
BstMBI GATC 6 cut(s) 83, 175, 273, 550, 695, 847
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 2 cut(s) 425, 1089
BstNI CCWGG 2 cut(s) 735, 872
BstNSI RCATGY 3 cut(s) 78, 93, 969
BstSCI CCNGG 2 cut(s) 733, 870
BstSLI GKGCMC 2 cut(s) 749, 1085
BstV1I GCAGC 2 cut(s) 55, 403
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 2 cut(s) 489, 520
BstYI RGATCY 1 cut(s) 550
BsuI GTATCC 1 cut(s) 921
BsuRI GGCC 1 cut(s) 481
BtgI CCRYGG 1 cut(s) 513
BtgZI GCGATG 2 cut(s) 409, 453
BtrI CACGTC 1 cut(s) 1080
BtsCI GGATG 3 cut(s) 107, 204, 419
BtsIMutI CAGTG 3 cut(s) 548, 668, 681
Cac8I GCNNGC 3 cut(s) 430, 653, 1112
Cfr13I GGNCC 1 cut(s) 454
Csp6I GTAC 1 cut(s) 988
CspCI CAANNNNNGTGG 2 cut(s) 826, 861
CviAII CATG 6 cut(s) 75, 90, 489, 582, 966, 1040
CviQI GTAC 1 cut(s) 988
DdeI CTNAG 2 cut(s) 434, 1064
DpnI GATC 6 cut(s) 85, 177, 275, 552, 697, 849
DpnII GATC 6 cut(s) 83, 175, 273, 550, 695, 847
DrdI GACNNNNNNGTC 2 cut(s) 236, 904
DseDI GACNNNNNNGTC 2 cut(s) 236, 904
EaeI YGGCCR 1 cut(s) 479
EciI GGCGGA 1 cut(s) 1089
Eco130I CCWWGG 2 cut(s) 565, 996
Eco47I GGWCC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 524
Eco88I CYCGRG 1 cut(s) 947
EcoO109I RGGNCCY 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 733, 870
EcoT14I CCWWGG 2 cut(s) 565, 996
ErhI CCWWGG 2 cut(s) 565, 996
FaeI CATG 6 cut(s) 78, 93, 492, 585, 969, 1043
FaqI GGGAC 1 cut(s) 242
FatI CATG 6 cut(s) 74, 89, 488, 581, 965, 1039
FauNDI CATATG 1 cut(s) 974
FbaI TGATCA 1 cut(s) 847
Fnu4HI GCNGC 4 cut(s) 6, 69, 417, 815
FokI GGATG 3 cut(s) 94, 191, 406
Fsp4HI GCNGC 4 cut(s) 6, 69, 417, 815
FspBI CTAG 3 cut(s) 131, 926, 1044
GluI GCNGC 4 cut(s) 6, 69, 417, 815
HaeIII GGCC 1 cut(s) 481
HapII CCGG 1 cut(s) 1103
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 6 cut(s) 78, 93, 492, 585, 969, 1043
HinfI GANTC 6 cut(s) 117, 341, 397, 905, 1011, 1072
HpaII CCGG 1 cut(s) 1103
HphI GGTGA 2 cut(s) 16, 336
Hpy166II GTNNAC 1 cut(s) 747
Hpy188I TCNGA 5 cut(s) 128, 371, 639, 897, 1077
Hpy188III TCNNGA 3 cut(s) 401, 554, 693
Hpy8I GTNNAC 1 cut(s) 747
Hpy99I CGWCG 2 cut(s) 563, 1081
HpyAV CCTTC 5 cut(s) 37, 62, 358, 391, 467
HpyCH4III ACNGT 2 cut(s) 306, 392
HpyCH4IV ACGT 5 cut(s) 410, 475, 485, 561, 1079
HpyCH4V TGCA 5 cut(s) 93, 419, 747, 1026, 1050
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 1089
HpyF3I CTNAG 2 cut(s) 434, 1064
HpySE526I ACGT 5 cut(s) 410, 475, 485, 561, 1079
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 6 cut(s) 78, 93, 492, 585, 969, 1043
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 6 cut(s) 83, 175, 273, 550, 695, 847
LmnI GCTCC 2 cut(s) 671, 871
Lsp1109I GCAGC 2 cut(s) 55, 403
LweI GCATC 1 cut(s) 428
MaeI CTAG 3 cut(s) 131, 926, 1044
MaeII ACGT 5 cut(s) 410, 475, 485, 561, 1079
MaeIII GTNAC 3 cut(s) 268, 317, 773
MalI GATC 6 cut(s) 85, 177, 275, 552, 697, 849
MboI GATC 6 cut(s) 83, 175, 273, 550, 695, 847
MboII GAAGA 7 cut(s) 22, 25, 28, 37, 40, 213, 666
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 3 cut(s) 668, 749, 1085
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 5 cut(s) 264, 711, 830, 916, 1118
MluNI TGGCCA 1 cut(s) 481
MlyI GAGTC 2 cut(s) 391, 914
MmeI TCCRAC 5 cut(s) 151, 706, 875, 946, 1100
Mox20I TGGCCA 1 cut(s) 481
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 1 cut(s) 481
MseI TTAA 1 cut(s) 359
MslI CAYNNNNRTG 1 cut(s) 541
Msp20I TGGCCA 1 cut(s) 481
MspA1I CMGCKG 2 cut(s) 68, 814
MspI CCGG 1 cut(s) 1103
MspR9I CCNGG 2 cut(s) 735, 872
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 735, 872
MwoI GCNNNNNNNGC 2 cut(s) 425, 1089
NdeI CATATG 1 cut(s) 974
NdeII GATC 6 cut(s) 83, 175, 273, 550, 695, 847
NlaIII CATG 6 cut(s) 78, 93, 492, 585, 969, 1043
NlaIV GGNNCC 1 cut(s) 525
NmuCI GTSAC 1 cut(s) 773
NspI RCATGY 3 cut(s) 78, 93, 969
OliI CACNNNNGTG 1 cut(s) 541
PciI ACATGT 1 cut(s) 965
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 4 cut(s) 117, 341, 1011, 1072
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 4 cut(s) 7, 70, 418, 816
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 2 cut(s) 391, 913
PpsI GAGTC 2 cut(s) 391, 913
PpuMI RGGWCCY 1 cut(s) 454
PscI ACATGT 1 cut(s) 965
PsiI TTATAA 1 cut(s) 1123
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 733, 870
PspGI CCWGG 2 cut(s) 733, 870
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 1 cut(s) 454
PspPPI RGGWCCY 1 cut(s) 454
PsuI RGATCY 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 560
PvuI CGATCG 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 989
RsaNI GTAC 1 cut(s) 988
RseI CAYNNNNRTG 1 cut(s) 541
SaqAI TTAA 1 cut(s) 359
SatI GCNGC 4 cut(s) 6, 69, 417, 815
Sau3AI GATC 6 cut(s) 83, 175, 273, 550, 695, 847
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 2 cut(s) 391, 914
ScrFI CCNGG 2 cut(s) 735, 872
SduI GDGCHC 3 cut(s) 668, 749, 1085
SfaNI GCATC 1 cut(s) 428
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 541
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
Sse9I AATT 5 cut(s) 264, 711, 830, 916, 1118
SsiI CCGC 3 cut(s) 814, 840, 1100
SspMI CTAG 3 cut(s) 131, 926, 1044
StyD4I CCNGG 2 cut(s) 733, 870
StyI CCWWGG 2 cut(s) 565, 996
TaaI ACNGT 2 cut(s) 306, 392
TaiI ACGT 5 cut(s) 413, 478, 488, 564, 1082
TaqI TCGA 6 cut(s) 82, 86, 115, 312, 395, 903
TasI AATT 5 cut(s) 264, 711, 830, 916, 1118
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 4 cut(s) 117, 341, 1011, 1072
Tru1I TTAA 1 cut(s) 359
Tru9I TTAA 1 cut(s) 359
TscAI CASTG 3 cut(s) 548, 668, 688
TseFI GTSAC 1 cut(s) 773
TseI GCWGC 3 cut(s) 5, 68, 416
Tsp45I GTSAC 1 cut(s) 773
TspDTI ATGAA 3 cut(s) 10, 563, 1028
TspGWI ACGGA 1 cut(s) 658
TspRI CASTG 3 cut(s) 548, 668, 688
Tth111I GACNNNGTC 1 cut(s) 560
VneI GTGCAC 1 cut(s) 745
VpaK11BI GGWCC 1 cut(s) 454
XceI RCATGY 3 cut(s) 78, 93, 969
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 3 cut(s) 131, 926, 1044
ZraI GACGTC 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.