FvH4_5g04551

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000675
Physical Location & Seq
Reverse (-)
2682784 .. 2684159
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04551.t1

Sequence Viewer

Length: 1170 bp
ATGGCTAATTGCTTCGAAATCTTCACCTCCTTACTCTTCTTCTCCTTCCTTCCACATCTAGCTTCCGCCACCTGCCGCCTCAACTGCAACTCCGACGGCCCACCTGTTCGATTCCCATTCTGGATAGTAGATCACGACTCCTCACACTGCGGGTGGCTAGGGTTTAATCTCTCATGCAACAACCGAAAGCAAACCATTCTGAATCTCCCATCTTCCGGGGATTTCATTCTCGACAACATTGACTACTATGACCAAATCATAACGATCAACGACACCAATAACTGCCTCATTAAGCGGTTCCTTGATGGTGAAATGAATCTCACGCCTTTACCCTTCTCCTACCTTTATGACTTTACAAACTACACATTCTTCAATTGCTCACCCGAAGCAAAAAACCTAGATCAATATCCAACCCTGTCTTGCCTCAGCAGTGACAACTACAAGGTCATTGTTGCGCCAACGAATGCTTTACTTAATGCAGACCTTGTTACTAGTCCACCTGCGATATCATCATCTCAGGTTCCGTCACCGTTTGATCCTAATTGCTCTGTGATTTCAACAGCTATGGTTCCAATAGAACCAAAGATTTATGATCCAATTCCATCCGAGTGGGAGGACATTAACTTTGGTGTTCAATTGCGGTGGGACGACCCTGATTGTCGGTATTGTACTTCGGTTGATCAAGCTTGTGGGATTGTTCATACGGGTATAACGGGTTCGGAAGTCGGGTGCATACCTGGCTCTACAAGACTTGCAAAGATTGCCACAGTGATAGGGTTGGTATTGTCAGGGGTATTGTGGATGGTTGGGCTTGCACTGAGACTGACAATGACAAGGACTAATAGTCGTCAACAGTCTACCACAGAGATGTCGATTTTAACGATTGATGAACAACTTCCGGTGGGCGTAACTGGTGGTCTTGATCAGAAAACGATTGATTCATATCCAAAGACTCAACTTGGTGACAGTCGGGAATTCCCCAGCTCCGGTGACAATGCCTGCCCTATATGTTTAGGAGAGTATCAGGCTAGGGAAACAGTAAGGACTATACCAGAATGCAACCACTATTTCCATGCTAATTGCGTAGACAAGTGGCTTAGAAGGAATCCGACTTGCCCACTTTGTCGAAATCCACCGGAGGGAGAACGGGATAAGCCATTGAGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

390

Amino Acids

43.26

Weight (kDa)

4.73

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 91 3.2e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 331 - 376 8.1e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 333 - 376 8.7e-15 Ring finger domain
zf-C3HC4_2 PF13923 334 - 375 3.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 334 - 362 2.9e-08 RING-like zinc finger
zf-C3HC4 PF00097 334 - 375 4e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 334 - 373 7.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 80, 508
Acc36I ACCTGC 2 cut(s) 80, 508
AccI GTMKAC 2 cut(s) 857, 1086
AciI CCGC 5 cut(s) 66, 76, 150, 295, 640
AclWI GGATC 2 cut(s) 530, 587
AcsI RAATTY 1 cut(s) 974
AfaI GTAC 1 cut(s) 670
AfiI CCNNNNNNNGG 3 cut(s) 215, 986, 1139
AgsI TTSAA 3 cut(s) 373, 558, 635
AhdI GACNNNNNGTC 1 cut(s) 843
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 1 cut(s) 736
AluBI AGCT 4 cut(s) 62, 563, 686, 984
AluI AGCT 4 cut(s) 62, 563, 686, 984
Alw26I GTCTC 1 cut(s) 814
AlwI GGATC 2 cut(s) 530, 587
AoxI GGCC 1 cut(s) 97
ApoI RAATTY 1 cut(s) 974
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 1 cut(s) 894
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 1 cut(s) 98
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 6 cut(s) 16, 320, 372, 519, 974, 1001
AsuII TTCGAA 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 425
BccI CCATC 4 cut(s) 217, 299, 610, 796
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 738
BclI TGATCA 2 cut(s) 679, 922
BcnI CCSGG 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 814
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 5 cut(s) 59, 158, 398, 492, 1029
BfuAI ACCTGC 2 cut(s) 80, 508
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 217, 738
BmeRI GACNNNNNGTC 1 cut(s) 843
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 3 cut(s) 299, 522, 570
BmrFI CCNGG 2 cut(s) 217, 738
Bpu10I CCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 217
BsaBI GATNNNNATC 2 cut(s) 405, 942
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 3 cut(s) 898, 986, 1135
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 986, 1139
Bse1I ACTGG 1 cut(s) 916
Bse8I GATNNNNATC 2 cut(s) 405, 942
BseBI CCWGG 1 cut(s) 738
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 602, 807
BseJI GATNNNNATC 2 cut(s) 405, 942
BseLI CCNNNNNNNGG 3 cut(s) 215, 986, 1139
BseMII CTCAG 3 cut(s) 439, 530, 809
BseNI ACTGG 1 cut(s) 916
BseRI GAGGAG 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 980
BshFI GGCC 1 cut(s) 99
BsiSI CCGG 4 cut(s) 216, 899, 987, 1136
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 3 cut(s) 215, 986, 1139
BsmAI GTCTC 1 cut(s) 814
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 2 cut(s) 469, 1061
BsnI GGCC 1 cut(s) 99
Bsp119I TTCGAA 1 cut(s) 15
Bsp143I GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
BspACI CCGC 5 cut(s) 66, 76, 150, 295, 640
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 3 cut(s) 438, 529, 810
BspLI GGNNCC 3 cut(s) 299, 522, 570
BspMI ACCTGC 2 cut(s) 80, 508
BspPI GGATC 2 cut(s) 530, 587
BspT104I TTCGAA 1 cut(s) 15
BsrI ACTGG 1 cut(s) 916
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
Bst2UI CCWGG 1 cut(s) 738
Bst4CI ACNGT 5 cut(s) 531, 769, 855, 968, 1039
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 761
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 813, 1000
BstDEI CTNAG 4 cut(s) 425, 516, 818, 1097
BstF5I GGATG 2 cut(s) 602, 807
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 7 cut(s) 133, 267, 403, 538, 595, 682, 925
BstMAI GTCTC 1 cut(s) 814
BstMBI GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
BstMWI GCNNNNNNNGC 3 cut(s) 84, 738, 761
BstNI CCWGG 1 cut(s) 738
BstSCI CCNGG 2 cut(s) 215, 736
BstXI CCANNNNNNTGG 1 cut(s) 609
BsuRI GGCC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 602, 807
BtsI GCAGTG 2 cut(s) 145, 436
BtsIMutI CAGTG 4 cut(s) 145, 436, 774, 815
BveI ACCTGC 2 cut(s) 80, 508
Cac8I GCNNGC 2 cut(s) 813, 1000
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 669
CspCI CAANNNNNGTGG 2 cut(s) 623, 658
CviAII CATG 2 cut(s) 174, 1073
CviQI GTAC 1 cut(s) 669
DdeI CTNAG 4 cut(s) 425, 516, 818, 1097
DpnI GATC 7 cut(s) 132, 266, 402, 537, 594, 681, 924
DpnII GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
DriI GACNNNNNGTC 1 cut(s) 843
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 55
Eco32I GATATC 1 cut(s) 507
EcoRI GAATTC 1 cut(s) 974
EcoRII CCWGG 1 cut(s) 736
EcoRV GATATC 1 cut(s) 507
FaeI CATG 2 cut(s) 177, 1076
FaqI GGGAC 1 cut(s) 659
FatI CATG 2 cut(s) 173, 1072
FauI CCCGC 1 cut(s) 143
FbaI TGATCA 2 cut(s) 679, 922
FblI GTMKAC 2 cut(s) 857, 1086
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 2 cut(s) 589, 814
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 5 cut(s) 59, 158, 398, 492, 1029
GlaI GCGC 1 cut(s) 456
GluI GCNGC 1 cut(s) 76
GsaI CCCAGC 1 cut(s) 984
HaeIII GGCC 1 cut(s) 99
HapII CCGG 4 cut(s) 216, 899, 987, 1136
HhaI GCGC 1 cut(s) 457
Hin1II CATG 2 cut(s) 177, 1076
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 1 cut(s) 851
HindII GTYRAC 1 cut(s) 851
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 7 cut(s) 111, 137, 202, 316, 938, 952, 1105
HpaII CCGG 4 cut(s) 216, 899, 987, 1136
HphI GGTGA 6 cut(s) 16, 320, 372, 519, 974, 1001
Hpy166II GTNNAC 4 cut(s) 497, 851, 858, 1087
Hpy188I TCNGA 6 cut(s) 94, 201, 607, 721, 927, 1110
Hpy188III TCNNGA 5 cut(s) 121, 134, 230, 920, 971
Hpy8I GTNNAC 4 cut(s) 497, 851, 858, 1087
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 4 cut(s) 55, 59, 343, 1095
HpyCH4III ACNGT 5 cut(s) 531, 769, 855, 968, 1039
HpyCH4V TGCA 7 cut(s) 87, 177, 479, 732, 755, 815, 1059
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 738, 761
HpyF3I CTNAG 4 cut(s) 425, 516, 818, 1097
Hsp92II CATG 2 cut(s) 177, 1076
HspAI GCGC 1 cut(s) 455
Ksp22I TGATCA 2 cut(s) 679, 922
Kzo9I GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
LmnI GCTCC 1 cut(s) 989
MaeI CTAG 5 cut(s) 59, 158, 398, 492, 1029
MaeIII GTNAC 7 cut(s) 431, 487, 525, 907, 962, 989, 1163
MalI GATC 7 cut(s) 132, 266, 402, 537, 594, 681, 924
MboI GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
MboII GAAGA 5 cut(s) 13, 28, 31, 204, 361
MfeI CAATTG 2 cut(s) 373, 635
MluCI AATT 7 cut(s) 7, 373, 541, 597, 635, 974, 1078
MlyI GAGTC 2 cut(s) 131, 946
MmeI TCCRAC 3 cut(s) 117, 434, 1133
MnlI CCTC 7 cut(s) 37, 89, 151, 296, 434, 607, 1132
MroXI GAANNNNTTC 1 cut(s) 894
MseI TTAA 5 cut(s) 165, 291, 474, 621, 878
MslI CAYNNNNRTG 2 cut(s) 607, 866
MspI CCGG 4 cut(s) 216, 899, 987, 1136
MspR9I CCNGG 2 cut(s) 217, 738
MunI CAATTG 2 cut(s) 373, 635
Mva1269I GAATGC 2 cut(s) 469, 1061
MvaI CCWGG 1 cut(s) 738
MwoI GCNNNNNNNGC 3 cut(s) 84, 738, 761
NciI CCSGG 1 cut(s) 217
NdeII GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
NlaIII CATG 2 cut(s) 177, 1076
NlaIV GGNNCC 3 cut(s) 299, 522, 570
NmuCI GTSAC 5 cut(s) 431, 525, 962, 989, 1163
NspV TTCGAA 1 cut(s) 15
PaqCI CACCTGC 2 cut(s) 80, 508
PcsI WCGNNNNNNNCGW 1 cut(s) 878
PctI GAATGC 2 cut(s) 469, 1061
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 5 cut(s) 111, 202, 316, 938, 1105
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 2 cut(s) 131, 946
PpsI GAGTC 2 cut(s) 131, 946
Psp6I CCWGG 1 cut(s) 736
PspFI CCCAGC 1 cut(s) 980
PspGI CCWGG 1 cut(s) 736
PspN4I GGNNCC 3 cut(s) 299, 522, 570
PspPI GGNCC 1 cut(s) 98
PsrI GAACNNNNNNTAC 2 cut(s) 700, 732
RsaI GTAC 1 cut(s) 670
RsaNI GTAC 1 cut(s) 669
RseI CAYNNNNRTG 2 cut(s) 607, 866
SaqAI TTAA 5 cut(s) 165, 291, 474, 621, 878
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 7 cut(s) 130, 264, 400, 535, 592, 679, 922
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 2 cut(s) 131, 946
ScrFI CCNGG 2 cut(s) 217, 738
SfuI TTCGAA 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 607, 866
SpeI ACTAGT 1 cut(s) 491
Sse9I AATT 7 cut(s) 7, 373, 541, 597, 635, 974, 1078
SsiI CCGC 5 cut(s) 66, 76, 150, 295, 640
SspMI CTAG 5 cut(s) 59, 158, 398, 492, 1029
StyD4I CCNGG 2 cut(s) 215, 736
TaaI ACNGT 5 cut(s) 531, 769, 855, 968, 1039
TaqI TCGA 5 cut(s) 15, 109, 231, 872, 1126
TasI AATT 7 cut(s) 7, 373, 541, 597, 635, 974, 1078
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 5 cut(s) 111, 202, 316, 938, 1105
Tru1I TTAA 5 cut(s) 165, 291, 474, 621, 878
Tru9I TTAA 5 cut(s) 165, 291, 474, 621, 878
TscAI CASTG 4 cut(s) 152, 436, 774, 822
TseFI GTSAC 5 cut(s) 431, 525, 962, 989, 1163
Tsp45I GTSAC 5 cut(s) 431, 525, 962, 989, 1163
TspDTI ATGAA 5 cut(s) 214, 329, 689, 903, 930
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 4 cut(s) 152, 436, 774, 822
XapI RAATTY 1 cut(s) 974
XmiI GTMKAC 2 cut(s) 857, 1086
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 5 cut(s) 59, 158, 398, 492, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.