Prupe.5G119000_v2.0.a1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000675
Physical Location & Seq
Reverse (-)
11975885 .. 11977300
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G119000.1

Sequence Viewer

Length: 1119 bp
ATGTCTACCTTACAATTCTTGATCACCTTCCTCTTGCTCTTCTTCTTCATTCATCATAACGGCGCAAGCGCCCCAATCTGTAGCGAATCTACGTGCTCTTTCAATAGCCACCCATGGATTCGATTCCCTTTCCGGCTGCAAAACCTTCAACCCTCCCTTTGTGGGTACTCAAAAAGCTTTGATCTCTCATGCAACAACCAAAATGAAGCCATTCTCACTCTCCCATCTTCCGGTGACTTCATAGTCAAAACCATAGATTACACTGGCCAAAACATATGGATCACTGACCTCAATAGCTGCTTCCCTAGGTTGTTCCTCGACCATGGCTTGAGCCTAGAAGGTTCACCTTTCTCATATGCGAATGATCTTGTAAACTTCACGCTCCTCAATTGCTCCTCTTTCGCAGAAACACCATATCCACCCATCCCTTGCCTTAGTAATAATGAGCAGTACGTGATCACTGCTGTGCCGTCGTCGGATTCTACGGTACCACCTTTGTGTTCTGTGTTTTCAACAGCTTTGGCTCCCCTGAGAGATCCACGCGATTGGAGCATTATTGATCTTTATGGTGTAGAGTTGGCATGGGATGTGCCCGATTGTCGTTTTTGTGTTGAATTGGGAAAATTTTGTGGGTTTGAGAATGCTAAAAGCTCCAAGCTTATGTGCTACGATATTTATAAGTATTATCCTTCAAATAACGATACAGCAAGAACTTCAGAGATTGCTCTAATAACATGCTTGGGAGTTCTAGGGCTACTTTTTACCACTGCGATTTTCCTAAGGCTCATGAGGACTTGTGGTCGGGTGCAGCAACCCATCGTGGAATTATCCACCATAACCAACCAACAATCTCCCGCAGTCATGAAGGGCCTTGATGATGCTACTATCGAGTCATATCCCAAGATTCAACTTGGTCAGAGTTGGGAATTGCCCAAGCCAAATGACAATACTTGCCCAATATGTTTAGGCACATACAAGTCCAAGGAAACATTGAGAACGATTCCAGAATGCAACCATTATTTCCATGCTAATTGCATAGACGAGTGGCTTAGAAGAAATGCGACGTGCCCGCTTTGTCGAAACCCTTCGGAGGGTAACAAAGTGATTATTGAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

373

Amino Acids

41.75

Weight (kDa)

5.19

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AasI GACNNNNNNGTC 1 cut(s) 242
Acc65I GGTACC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 543
AciI CCGC 2 cut(s) 855, 1070
AclWI GGATC 2 cut(s) 287, 530
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 3 cut(s) 167, 452, 489
AfiI CCNNNNNNNGG 4 cut(s) 162, 230, 1090, 1091
AgsI TTSAA 6 cut(s) 103, 149, 513, 614, 693, 908
AhdI GACNNNNNGTC 1 cut(s) 798
AjiI CACGTC 1 cut(s) 1065
AleI CACNNNNGTG 2 cut(s) 464, 496
AluBI AGCT 5 cut(s) 177, 297, 518, 651, 658
AluI AGCT 5 cut(s) 177, 297, 518, 651, 658
Alw21I GWGCWC 1 cut(s) 98
AlwI GGATC 2 cut(s) 287, 530
AoxI GGCC 2 cut(s) 265, 868
ApeKI GCWGC 3 cut(s) 136, 297, 808
ApoI RAATTY 1 cut(s) 623
Asp700I GAANNNNTTC 2 cut(s) 210, 1084
Asp718I GGTACC 1 cut(s) 487
AspA2I CCTAGG 1 cut(s) 305
AspLEI GCGC 2 cut(s) 65, 71
AspS9I GGNCC 1 cut(s) 868
AsuHPI GGTGA 3 cut(s) 16, 245, 336
AvrII CCTAGG 1 cut(s) 305
AxyI CCTNAGG 1 cut(s) 779
BaeGI GKGCMC 2 cut(s) 594, 1070
BalI TGGCCA 1 cut(s) 267
BanI GGYRCC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 123, 284, 820
BccI CCATC 3 cut(s) 232, 431, 824
BceAI ACGGC 2 cut(s) 76, 454
BclI TGATCA 2 cut(s) 21, 456
BfaI CTAG 3 cut(s) 306, 335, 749
BfmI CTRYAG 1 cut(s) 79
BfoI RGCGCY 1 cut(s) 72
BisI GCNGC 3 cut(s) 137, 298, 809
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 3 cut(s) 138, 299, 810
BmeRI GACNNNNNGTC 1 cut(s) 798
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 1 cut(s) 868
BmiI GGNNCC 2 cut(s) 489, 525
BmsI GCATC 1 cut(s) 868
BpuEI CTTGAG 1 cut(s) 349
BsaAI YACGTR 2 cut(s) 93, 454
BsaJI CCNNGG 4 cut(s) 113, 305, 322, 981
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 230, 1090, 1091
Bse1I ACTGG 1 cut(s) 268
Bse21I CCTNAGG 1 cut(s) 779
BseDI CCNNGG 4 cut(s) 113, 305, 322, 981
BseGI GGATG 2 cut(s) 423, 592
BseLI CCNNNNNNNGG 4 cut(s) 162, 230, 1090, 1091
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 2 cut(s) 374, 385
BseSI GKGCMC 2 cut(s) 594, 1070
BseXI GCAGC 3 cut(s) 123, 284, 820
BsgI GTGCAG 1 cut(s) 827
Bsh1236I CGCG 1 cut(s) 543
BshFI GGCC 2 cut(s) 267, 870
BshNI GGYRCC 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 2 cut(s) 133, 231
BslI CCNNNNNNNGG 4 cut(s) 162, 230, 1090, 1091
BsmI GAATGC 2 cut(s) 646, 1013
BsnI GGCC 2 cut(s) 267, 870
Bsp1286I GDGCHC 3 cut(s) 98, 594, 1070
Bsp143I GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
Bsp19I CCATGG 2 cut(s) 113, 322
BspACI CCGC 2 cut(s) 855, 1070
BspANI GGCC 2 cut(s) 267, 870
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 1 cut(s) 543
BspHI TCATGA 2 cut(s) 786, 861
BspLI GGNNCC 2 cut(s) 489, 525
BspPI GGATC 2 cut(s) 287, 530
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 487
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 4 cut(s) 113, 305, 322, 981
BssMI GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
BssT1I CCWWGG 4 cut(s) 113, 305, 322, 981
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 1 cut(s) 44
BstBAI YACGTR 2 cut(s) 93, 454
BstC8I GCNNGC 2 cut(s) 67, 1070
BstDEI CTNAG 4 cut(s) 434, 530, 779, 1049
BstDSI CCRYGG 2 cut(s) 113, 322
BstF5I GGATG 2 cut(s) 423, 592
BstFNI CGCG 1 cut(s) 543
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 2 cut(s) 65, 71
BstKTI GATC 7 cut(s) 24, 184, 282, 367, 459, 538, 562
BstMBI GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
BstMWI GCNNNNNNNGC 1 cut(s) 549
BstNSI RCATGY 1 cut(s) 738
BstSFI CTRYAG 1 cut(s) 79
BstSLI GKGCMC 2 cut(s) 594, 1070
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 3 cut(s) 123, 284, 820
BstX2I RGATCY 1 cut(s) 535
BstXI CCANNNNNNTGG 1 cut(s) 546
BstYI RGATCY 1 cut(s) 535
Bsu36I CCTNAGG 1 cut(s) 779
BsuRI GGCC 2 cut(s) 267, 870
BtgI CCRYGG 2 cut(s) 113, 322
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 2 cut(s) 423, 592
BtsI GCAGTG 2 cut(s) 459, 765
BtsIMutI CAGTG 4 cut(s) 261, 282, 459, 765
Cac8I GCNNGC 2 cut(s) 67, 1070
CciI TCATGA 2 cut(s) 786, 861
CfoI GCGC 2 cut(s) 65, 71
Cfr13I GGNCC 1 cut(s) 868
Csp6I GTAC 3 cut(s) 166, 451, 488
CviAII CATG 8 cut(s) 114, 189, 323, 582, 735, 787, 862, 1025
CviQI GTAC 3 cut(s) 166, 451, 488
DdeI CTNAG 4 cut(s) 434, 530, 779, 1049
DpnI GATC 7 cut(s) 23, 183, 281, 366, 458, 537, 561
DpnII GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
DrdI GACNNNNNNGTC 1 cut(s) 242
DriI GACNNNNNGTC 1 cut(s) 798
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 44
Eam1105I GACNNNNNGTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 44
Eco130I CCWWGG 4 cut(s) 113, 305, 322, 981
Eco57I CTGAAG 1 cut(s) 699
Eco81I CCTNAGG 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 868
EcoT14I CCWWGG 4 cut(s) 113, 305, 322, 981
ErhI CCWWGG 4 cut(s) 113, 305, 322, 981
FaeI CATG 8 cut(s) 117, 192, 326, 585, 738, 790, 865, 1028
FatI CATG 8 cut(s) 113, 188, 322, 581, 734, 786, 861, 1024
FauI CCCGC 2 cut(s) 862, 1077
FauNDI CATATG 2 cut(s) 275, 355
FbaI TGATCA 2 cut(s) 21, 456
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 137, 298, 809
FokI GGATG 2 cut(s) 410, 599
Fsp4HI GCNGC 3 cut(s) 137, 298, 809
FspBI CTAG 3 cut(s) 306, 335, 749
GlaI GCGC 2 cut(s) 64, 70
GluI GCNGC 3 cut(s) 137, 298, 809
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 2 cut(s) 267, 870
HapII CCGG 2 cut(s) 133, 231
HhaI GCGC 2 cut(s) 65, 71
Hin1II CATG 8 cut(s) 117, 192, 326, 585, 738, 790, 865, 1028
Hin6I GCGC 2 cut(s) 63, 69
HinP1I GCGC 2 cut(s) 63, 69
HindIII AAGCTT 2 cut(s) 175, 656
HinfI GANTC 7 cut(s) 86, 118, 123, 479, 890, 904, 1000
HpaII CCGG 2 cut(s) 133, 231
HphI GGTGA 3 cut(s) 16, 245, 336
Hpy166II GTNNAC 3 cut(s) 6, 344, 373
Hpy188I TCNGA 4 cut(s) 478, 718, 918, 1090
Hpy188III TCNNGA 4 cut(s) 19, 787, 862, 1004
Hpy8I GTNNAC 3 cut(s) 6, 344, 373
Hpy99I CGWCG 3 cut(s) 475, 478, 1066
HpyAV CCTTC 6 cut(s) 37, 155, 332, 699, 859, 1095
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 3 cut(s) 92, 453, 1064
HpyCH4V TGCA 5 cut(s) 139, 192, 808, 1011, 1035
HpyF10VI GCNNNNNNNGC 1 cut(s) 549
HpyF3I CTNAG 4 cut(s) 434, 530, 779, 1049
HpySE526I ACGT 3 cut(s) 92, 453, 1064
Hsp92II CATG 8 cut(s) 117, 192, 326, 585, 738, 790, 865, 1028
HspAI GCGC 2 cut(s) 63, 69
KpnI GGTACC 1 cut(s) 491
Ksp22I TGATCA 2 cut(s) 21, 456
Kzo9I GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 5 cut(s) 387, 398, 529, 549, 656
LpnPI CCDG 5 cut(s) 146, 244, 249, 542, 1017
Lsp1109I GCAGC 3 cut(s) 123, 284, 820
LweI GCATC 1 cut(s) 868
MaeI CTAG 3 cut(s) 306, 335, 749
MaeII ACGT 3 cut(s) 92, 453, 1064
MaeIII GTNAC 2 cut(s) 233, 1094
MalI GATC 7 cut(s) 23, 183, 281, 366, 458, 537, 561
MboI GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
MboII GAAGA 5 cut(s) 31, 34, 37, 219, 1065
MfeI CAATTG 1 cut(s) 388
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 3 cut(s) 98, 594, 1070
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 7 cut(s) 14, 388, 614, 623, 824, 926, 1030
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 9 cut(s) 41, 163, 299, 326, 395, 406, 783, 1084, 1105
Mox20I TGGCCA 1 cut(s) 267
MroXI GAANNNNTTC 2 cut(s) 210, 1084
MscI TGGCCA 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 464, 496
Msp20I TGGCCA 1 cut(s) 267
MspI CCGG 2 cut(s) 133, 231
MunI CAATTG 1 cut(s) 388
Mva1269I GAATGC 2 cut(s) 646, 1013
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 1 cut(s) 549
NcoI CCATGG 2 cut(s) 113, 322
NdeI CATATG 2 cut(s) 275, 355
NdeII GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
NlaIII CATG 8 cut(s) 117, 192, 326, 585, 738, 790, 865, 1028
NlaIV GGNNCC 2 cut(s) 489, 525
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 738
OliI CACNNNNGTG 2 cut(s) 464, 496
PagI TCATGA 2 cut(s) 786, 861
PciSI GCTCTTC 1 cut(s) 44
PctI GAATGC 2 cut(s) 646, 1013
PdmI GAANNNNTTC 2 cut(s) 210, 1084
PfeI GAWTC 6 cut(s) 86, 118, 123, 479, 904, 1000
PkrI GCNGC 3 cut(s) 138, 299, 810
PleI GAGTC 1 cut(s) 898
PpsI GAGTC 1 cut(s) 898
Ppu21I YACGTR 2 cut(s) 93, 454
PsiI TTATAA 1 cut(s) 678
PspN4I GGNNCC 2 cut(s) 489, 525
PspPI GGNCC 1 cut(s) 868
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 3 cut(s) 167, 452, 489
RsaNI GTAC 3 cut(s) 166, 451, 488
RseI CAYNNNNRTG 2 cut(s) 464, 496
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 3 cut(s) 137, 298, 809
Sau3AI GATC 7 cut(s) 21, 181, 279, 364, 456, 535, 559
Sau96I GGNCC 1 cut(s) 868
SchI GAGTC 1 cut(s) 899
SduI GDGCHC 3 cut(s) 98, 594, 1070
SfaNI GCATC 1 cut(s) 868
SfcI CTRYAG 1 cut(s) 79
SmiMI CAYNNNNRTG 2 cut(s) 464, 496
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
Sse9I AATT 7 cut(s) 14, 388, 614, 623, 824, 926, 1030
SsiI CCGC 2 cut(s) 855, 1070
SspMI CTAG 3 cut(s) 306, 335, 749
StyI CCWWGG 4 cut(s) 113, 305, 322, 981
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 3 cut(s) 95, 456, 1067
TaqI TCGA 4 cut(s) 121, 318, 888, 1078
TasI AATT 7 cut(s) 14, 388, 614, 623, 824, 926, 1030
TfiI GAWTC 6 cut(s) 86, 118, 123, 479, 904, 1000
TscAI CASTG 4 cut(s) 268, 289, 466, 772
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 136, 297, 808
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 5 cut(s) 37, 41, 219, 229, 878
TspRI CASTG 4 cut(s) 268, 289, 466, 772
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 738
XmaJI CCTAGG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 210, 1084
XspI CTAG 3 cut(s) 306, 335, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.