RchiOBHm_Chr7g0226371

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000675
Physical Location & Seq
Forward (+)
49567643 .. 49568959
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20276

Sequence Viewer

Length: 1140 bp
ATGGCAACCTCAGACATATTATTCTCATTCTTCTTCTTCTTCTTCACCTTCTTACTTCCTCAGATAGCAGATAGCAGATGTGGTGGTTCAACATGCTATGGGAGCGGCCCACTGATTCGATTCCCTTTCCGGCTAAGATACCGCCAACCGCTAAATTGTGGGTATTCCATTCATTTTGACCTTTCATGCAACAGTACACAAAAAAGCACCATTCTCACCCTCCCTTCTTCAGGAGACTTCGAGGTCGAGAGCATCCATTACAGTGACCAAACCATGTGGATCAACGACCTAAACTGGTGCATGCACAAAAGATTCCTTGACAAAGACTTCGGTCTTGTAGGCACGCCTTTCCATTTCTGGCATAGCCTGGAAAACTATACCTACTTCAAGTGCCCGTCCAAAGTTGTGGCTATTGGGTCTTTTGCACCAATCTCTTGCCTTGACGATTACAATTACAACGTCATTGCTGTCCCGTCGAGTTGGTCAACGACTGAGAAACCAGCTCCTCAGTCTCTTTGTCTTGCAATTTCCACAGCTTTGCTTCCGGCGAGTTTTGGGTGGGAGGAGTTTGCTGTAAAGTTGACTTGGGGCGTTCCCGATTGTCGTTCTTGTGAAGCAAATGGTAGAGTTTGTAAGCCTAATGTGACAAGTTCGCACATTGAGTGCTCAACTGATGGTATCCCAATATCCTCAAAGACCGGCATAGCAATTAGCGTGTTGATTCCGGCAGGGCTGTTGTGCATTGTTGGATACGCACTCTATGTTGCGGGCAGGAGGGATGATGGTCTGGTCCAAGAAACAAACAGGCGTGGTCAGCTTCAAGAACTCATTCAAGCCAATGGACAACCTCAAGTAGTTACAGCGGGTCTTGATGCTCAGACTATTGAGTCATATTTGAAGACTCAACTTGGTGAGAGTTTAGAATTACCTGACCCTAATAACAAAACTTGCTCGATATGTTTGGGTGAGTACCAGTCCAAGGAAATGCTAAGGACTATACCAGAATGCAATCACTATTTTCATGTTGGTTGCATAGACGAGTGGCTTAGAAAGAACCCAACATGTCCACTTTGTCGAAATCTTCCTCGAACACCCGAAGTGTCATTAGTGAGACCTCACGATGATCATTCTATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

379

Amino Acids

42.39

Weight (kDa)

5.81

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 96 5.9e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 315 - 359 6.8e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 316 - 359 3.5e-14 Ring finger domain
zf-RING_11 PF17123 317 - 345 7e-09 RING-like zinc finger
zf-C3HC4 PF00097 317 - 358 8.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 317 - 358 9.3e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 242, 886
AccBSI CCGCTC 1 cut(s) 105
AciI CCGC 5 cut(s) 105, 142, 149, 767, 863
AclWI GGATC 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 2 cut(s) 196, 971
AfiI CCNNNNNNNGG 2 cut(s) 230, 979
AflIII ACRYGT 1 cut(s) 1061
AgsI TTSAA 5 cut(s) 90, 388, 821, 833, 898
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 3 cut(s) 503, 536, 817
AluI AGCT 3 cut(s) 503, 536, 817
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 3 cut(s) 228, 516, 1105
AlwI GGATC 1 cut(s) 287
AoxI GGCC 1 cut(s) 106
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
Asp700I GAANNNNTTC 1 cut(s) 828
AspS9I GGNCC 2 cut(s) 107, 790
AsuHPI GGTGA 4 cut(s) 37, 208, 923, 977
AvaII GGWCC 1 cut(s) 790
BaeGI GKGCMC 1 cut(s) 395
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 668
BccI CCATC 2 cut(s) 668, 776
BciT130I CCWGG 1 cut(s) 368
BciVI GTATCC 2 cut(s) 689, 743
BclI TGATCA 1 cut(s) 1123
BcoDI GTCTC 3 cut(s) 228, 516, 1105
BfuI GTATCC 2 cut(s) 689, 743
BisI GCNGC 1 cut(s) 106
BlsI GCNGC 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 790
BmgT120I GGNCC 2 cut(s) 107, 790
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 2 cut(s) 261, 862
BoxI GACNNNNGTC 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 905
Bpu10I CCTNAGC 1 cut(s) 989
BpuEI CTTGAG 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 1105
BsaJI CCNNGG 1 cut(s) 978
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 979
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 2 cut(s) 299, 973
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 978
BseGI GGATG 2 cut(s) 252, 784
BseLI CCNNNNNNNGG 2 cut(s) 230, 979
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 5 cut(s) 24, 74, 483, 521, 890
BseNI ACTGG 2 cut(s) 299, 973
BseRI GAGGAG 2 cut(s) 495, 578
BseSI GKGCMC 1 cut(s) 395
BshFI GGCC 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 668
BsiSI CCGG 4 cut(s) 130, 545, 699, 725
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 2 cut(s) 230, 979
BsmAI GTCTC 3 cut(s) 228, 516, 1105
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 1105
Bsp1286I GDGCHC 2 cut(s) 395, 668
Bsp143I GATC 2 cut(s) 279, 1123
BspACI CCGC 5 cut(s) 105, 142, 149, 767, 863
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 5 cut(s) 23, 73, 484, 520, 889
BspPI GGATC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 1105
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 2 cut(s) 299, 973
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 1 cut(s) 978
BssMI GATC 2 cut(s) 279, 1123
BssT1I CCWWGG 1 cut(s) 978
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 194, 263
BstC8I GCNNGC 3 cut(s) 302, 344, 769
BstDEI CTNAG 8 cut(s) 10, 60, 134, 492, 507, 876, 989, 1046
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 252, 784
BstKTI GATC 2 cut(s) 282, 1126
BstMAI GTCTC 3 cut(s) 228, 516, 1105
BstMBI GATC 2 cut(s) 279, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 102, 814
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 3 cut(s) 96, 304, 1065
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 366
BstSLI GKGCMC 1 cut(s) 395
BstV2I GAAGAC 1 cut(s) 905
BstXI CCANNNNNNTGG 1 cut(s) 406
BsuI GTATCC 2 cut(s) 689, 743
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 252, 784
BtsIMutI CAGTG 2 cut(s) 110, 268
Cac8I GCNNGC 3 cut(s) 302, 344, 769
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 2 cut(s) 107, 790
Csp6I GTAC 2 cut(s) 195, 970
CspCI CAANNNNNGTGG 2 cut(s) 257, 292
CviAII CATG 6 cut(s) 93, 186, 274, 301, 1022, 1062
CviQI GTAC 2 cut(s) 195, 970
DdeI CTNAG 8 cut(s) 10, 60, 134, 492, 507, 876, 989, 1046
DpnI GATC 2 cut(s) 281, 1125
DpnII GATC 2 cut(s) 279, 1123
DrdI GACNNNNNNGTC 2 cut(s) 242, 886
DseDI GACNNNNNNGTC 2 cut(s) 242, 886
Eco130I CCWWGG 1 cut(s) 978
Eco31I GGTCTC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 790
Eco57I CTGAAG 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 978
ErhI CCWWGG 1 cut(s) 978
FaeI CATG 6 cut(s) 96, 189, 277, 304, 1025, 1065
FaqI GGGAC 1 cut(s) 455
FatI CATG 6 cut(s) 92, 185, 273, 300, 1021, 1061
FauI CCCGC 2 cut(s) 760, 856
FbaI TGATCA 1 cut(s) 1123
Fnu4HI GCNGC 1 cut(s) 106
FokI GGATG 2 cut(s) 239, 791
Fsp4HI GCNGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 106
HaeIII GGCC 1 cut(s) 108
HapII CCGG 4 cut(s) 130, 545, 699, 725
Hin1II CATG 6 cut(s) 96, 189, 277, 304, 1025, 1065
HincII GTYRAC 2 cut(s) 486, 582
HindII GTYRAC 2 cut(s) 486, 582
HinfI GANTC 6 cut(s) 115, 120, 312, 721, 887, 901
HpaII CCGG 4 cut(s) 130, 545, 699, 725
HphI GGTGA 4 cut(s) 37, 208, 923, 977
Hpy166II GTNNAC 4 cut(s) 197, 486, 582, 1067
Hpy188I TCNGA 3 cut(s) 13, 63, 879
Hpy188III TCNNGA 6 cut(s) 231, 247, 596, 821, 869, 1118
Hpy8I GTNNAC 4 cut(s) 197, 486, 582, 1067
Hpy99I CGWCG 1 cut(s) 478
HpyAV CCTTC 2 cut(s) 58, 234
HpyCH4III ACNGT 2 cut(s) 194, 263
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 8 cut(s) 189, 300, 304, 425, 524, 741, 1008, 1032
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 814
HpyF3I CTNAG 8 cut(s) 10, 60, 134, 492, 507, 876, 989, 1046
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 6 cut(s) 96, 189, 277, 304, 1025, 1065
Ksp22I TGATCA 1 cut(s) 1123
Kzo9I GATC 2 cut(s) 279, 1123
LmnI GCTCC 2 cut(s) 102, 508
LweI GCATC 2 cut(s) 261, 862
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 263, 643, 856
MalI GATC 2 cut(s) 281, 1125
MbiI CCGCTC 1 cut(s) 105
MboI GATC 2 cut(s) 279, 1123
MboII GAAGA 8 cut(s) 22, 25, 28, 31, 34, 219, 910, 1073
MhlI GDGCHC 2 cut(s) 395, 668
MluCI AATT 5 cut(s) 154, 451, 525, 708, 923
MlyI GAGTC 2 cut(s) 895, 896
MmeI TCCRAC 1 cut(s) 727
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 1 cut(s) 1138
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 4 cut(s) 130, 545, 699, 725
MspR9I CCNGG 1 cut(s) 368
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 102, 814
NdeII GATC 2 cut(s) 279, 1123
NlaIII CATG 6 cut(s) 96, 189, 277, 304, 1025, 1065
NmuCI GTSAC 2 cut(s) 263, 643
NspI RCATGY 3 cut(s) 96, 304, 1065
PaeI GCATGC 1 cut(s) 304
PciI ACATGT 1 cut(s) 1061
PctI GAATGC 1 cut(s) 1010
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 4 cut(s) 115, 120, 312, 721
PkrI GCNGC 1 cut(s) 107
PleI GAGTC 2 cut(s) 895, 895
PpsI GAGTC 2 cut(s) 895, 895
PscI ACATGT 1 cut(s) 1061
PshAI GACNNNNGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspPI GGNCC 2 cut(s) 107, 790
RsaI GTAC 2 cut(s) 196, 971
RsaNI GTAC 2 cut(s) 195, 970
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 1 cut(s) 1138
SatI GCNGC 1 cut(s) 106
Sau3AI GATC 2 cut(s) 279, 1123
Sau96I GGNCC 2 cut(s) 107, 790
SchI GAGTC 2 cut(s) 895, 896
ScrFI CCNGG 1 cut(s) 368
SduI GDGCHC 2 cut(s) 395, 668
SfaNI GCATC 2 cut(s) 261, 862
SinI GGWCC 1 cut(s) 790
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
SphI GCATGC 1 cut(s) 304
Sse9I AATT 5 cut(s) 154, 451, 525, 708, 923
SsiI CCGC 5 cut(s) 105, 142, 149, 767, 863
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 978
TaaI ACNGT 2 cut(s) 194, 263
TaiI ACGT 1 cut(s) 462
TaqI TCGA 7 cut(s) 118, 240, 246, 476, 953, 1075, 1087
TaqII GACCGA 1 cut(s) 320
TasI AATT 5 cut(s) 154, 451, 525, 708, 923
TatI WGTACW 1 cut(s) 194
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 4 cut(s) 115, 120, 312, 721
Tru1I TTAA 1 cut(s) 1138
Tru9I TTAA 1 cut(s) 1138
TscAI CASTG 2 cut(s) 117, 268
TseFI GTSAC 2 cut(s) 263, 643
Tsp45I GTSAC 2 cut(s) 263, 643
TspDTI ATGAA 3 cut(s) 161, 174, 1010
TspRI CASTG 2 cut(s) 117, 268
VpaK11BI GGWCC 1 cut(s) 790
XagI CCTNNNNNAGG 1 cut(s) 228
XceI RCATGY 3 cut(s) 96, 304, 1065
XmnI GAANNNNTTC 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.