RLG00000001745

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000675
Physical Location & Seq
Reverse (-)
20027480 .. 20028794
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001745

Sequence Viewer

Length: 1134 bp
ATGGCCTCAGACATATTATTCTCATTCTTCTTCTTCTTCACCTTCTTACTTGCTCAGATAGCAGATAGCAGATGTGGTGGTTCAAAATGCTATGGGAGCGGCCCACTGATTCGATTCCCTTTCCGGCTAAGATACCGCCAACCGCTAAATTGTGGGTATTCCATTCATTTTGACCTTTCATGCAACAGTACACAAAAAAACACCATTCTCACCCTCCCTTCTTCAGGAGACTTCGAGGTCGAGAGCATCCATTACACTGACCAAACCATGTGGATCAACGACCTAAACTGGTGCATGCACAAAAGATTTCTTGACAAAGACTTCAGTCTTGTAGGCACGCCTTTCCATTTCTGGCATAGCCTGGAAAACTATACCTACTTCAAGTGCCCGTCCAAAGTTGTGGCTATTGGGTCTTTTGCACCAATCTCTTGCCTTGGCGATTACAATTACAACGTCATTGCTGTCCCGTCGAGTTGGTCAACGACTGAGAAACCACCTCGTCAGTCTCTTTGTCGTGCAATTTCCACAGCTTTGCTTCCGGCGAGTTTTGGGTGGGGGGAGTTTGCTGTAAAGTTGACTTGGGGCGTTCCCGATTGTCGTTCTTGTGAAGCAACTGGTAGAGTTTGTAGGCCTACTGTGGCAAGTTCGCACATTGAGTGCTCAAATGATGGTATCCTAATATCCGCAAAAACCGGCATAGCAATTGGCGTGTTGATTCCGGCAGGGCTGTTGTGCATTGTTGGATACGCACTCTATGTTGCTGGCAGGAGGGATGATGGTCGGGTCCAAGAAACAAACAGGCGTGGTCAGCTTCAAGAACTCATTGAAGCCAATGGACAACCTCAAGTAGTTACAGCGGGTCTTGATGCTCAGACTATTGAGTCATATTTGAAGACTCAACTTGGTGAGAATTTAGAATTACCTGACCCTAATGACAAAACCTGCTCGATATGTTTGGGTGAGTACCAGTCCAAGGAAATGCTAAGGACTATACCAGAATGCAGTCATTATTTTCATGTTGGTTGCATAGACGAGTGGCTTAGAAAGAACCCGACATGTCCACTTTGTCGAAATCTTCCTCGAACACCCGAAGTGTCATTAGTGAGACCGCATGATGATCATTCTATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

378

Amino Acids

42.21

Weight (kDa)

6.36

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 94 3.5e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 313 - 357 9.1e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 314 - 357 3.7e-14 Ring finger domain
zf-RING_11 PF17123 315 - 343 3.8e-09 RING-like zinc finger
zf-C3HC4 PF00097 315 - 356 1.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 315 - 356 1.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 236, 880
Acc36I ACCTGC 1 cut(s) 950
AccBSI CCGCTC 1 cut(s) 99
AciI CCGC 6 cut(s) 99, 136, 143, 684, 857, 1109
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 910
AcuI CTGAAG 2 cut(s) 207, 307
AfaI GTAC 2 cut(s) 190, 965
AfiI CCNNNNNNNGG 2 cut(s) 224, 973
AflIII ACRYGT 1 cut(s) 1055
AgsI TTSAA 5 cut(s) 84, 382, 815, 827, 892
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 2 cut(s) 530, 811
AluI AGCT 2 cut(s) 530, 811
Alw21I GWGCWC 1 cut(s) 662
Alw26I GTCTC 3 cut(s) 222, 510, 1099
AlwI GGATC 1 cut(s) 281
AoxI GGCC 3 cut(s) 3, 100, 629
ApoI RAATTY 1 cut(s) 910
AspS9I GGNCC 2 cut(s) 101, 784
AsuHPI GGTGA 4 cut(s) 31, 202, 917, 971
AvaII GGWCC 1 cut(s) 784
BaeGI GKGCMC 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 662
BccI CCATC 2 cut(s) 662, 770
BciT130I CCWGG 1 cut(s) 362
BciVI GTATCC 2 cut(s) 683, 737
BclI TGATCA 1 cut(s) 1117
BcoDI GTCTC 3 cut(s) 222, 510, 1099
BfuAI ACCTGC 1 cut(s) 950
BfuI GTATCC 2 cut(s) 683, 737
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 784
BmgT120I GGNCC 2 cut(s) 101, 784
BmiI GGNNCC 1 cut(s) 785
BmrFI CCNGG 1 cut(s) 362
BmsI GCATC 2 cut(s) 255, 856
BoxI GACNNNNGTC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 899
Bpu10I CCTNAGC 1 cut(s) 983
BpuEI CTTGAG 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 1099
BsaJI CCNNGG 2 cut(s) 433, 972
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 973
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 3 cut(s) 293, 619, 967
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 1 cut(s) 362
BseDI CCNNGG 2 cut(s) 433, 972
BseGI GGATG 2 cut(s) 246, 778
BseLI CCNNNNNNNGG 2 cut(s) 224, 973
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 4 cut(s) 21, 68, 477, 884
BseNI ACTGG 3 cut(s) 293, 619, 967
BseSI GKGCMC 1 cut(s) 389
BshFI GGCC 3 cut(s) 5, 102, 631
BsiHKAI GWGCWC 1 cut(s) 662
BsiSI CCGG 4 cut(s) 124, 539, 693, 719
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 2 cut(s) 224, 973
BsmAI GTCTC 3 cut(s) 222, 510, 1099
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 1 cut(s) 1004
BsnI GGCC 3 cut(s) 5, 102, 631
Bso31I GGTCTC 1 cut(s) 1099
Bsp1286I GDGCHC 2 cut(s) 389, 662
Bsp143I GATC 2 cut(s) 273, 1117
BspACI CCGC 6 cut(s) 99, 136, 143, 684, 857, 1109
BspANI GGCC 3 cut(s) 5, 102, 631
BspCNI CTCAG 4 cut(s) 20, 67, 478, 883
BspLI GGNNCC 1 cut(s) 785
BspMI ACCTGC 1 cut(s) 950
BspPI GGATC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 1099
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 3 cut(s) 293, 619, 967
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 2 cut(s) 433, 972
BssMI GATC 2 cut(s) 273, 1117
BssT1I CCWWGG 2 cut(s) 433, 972
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 188, 637
BstC8I GCNNGC 3 cut(s) 296, 338, 763
BstDEI CTNAG 7 cut(s) 7, 54, 128, 486, 870, 983, 1040
BstENI CCTNNNNNAGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 246, 778
BstKTI GATC 2 cut(s) 276, 1120
BstMAI GTCTC 3 cut(s) 222, 510, 1099
BstMBI GATC 2 cut(s) 273, 1117
BstMWI GCNNNNNNNGC 3 cut(s) 59, 96, 808
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 2 cut(s) 298, 1059
BstPAI GACNNNNGTC 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 389
BstV2I GAAGAC 1 cut(s) 899
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuI GTATCC 2 cut(s) 683, 737
BsuRI GGCC 3 cut(s) 5, 102, 631
BtsCI GGATG 2 cut(s) 246, 778
BtsIMutI CAGTG 2 cut(s) 104, 255
BveI ACCTGC 1 cut(s) 950
Cac8I GCNNGC 3 cut(s) 296, 338, 763
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 2 cut(s) 101, 784
Csp6I GTAC 2 cut(s) 189, 964
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 6 cut(s) 180, 268, 295, 1016, 1056, 1112
CviQI GTAC 2 cut(s) 189, 964
DdeI CTNAG 7 cut(s) 7, 54, 128, 486, 870, 983, 1040
DpnI GATC 2 cut(s) 275, 1119
DpnII GATC 2 cut(s) 273, 1117
DrdI GACNNNNNNGTC 2 cut(s) 236, 880
DseDI GACNNNNNNGTC 2 cut(s) 236, 880
Eco130I CCWWGG 2 cut(s) 433, 972
Eco147I AGGCCT 1 cut(s) 631
Eco31I GGTCTC 1 cut(s) 1099
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 207, 307
EcoNI CCTNNNNNAGG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 433, 972
ErhI CCWWGG 2 cut(s) 433, 972
FaeI CATG 6 cut(s) 183, 271, 298, 1019, 1059, 1115
FaqI GGGAC 1 cut(s) 449
FatI CATG 6 cut(s) 179, 267, 294, 1015, 1055, 1111
FauI CCCGC 1 cut(s) 850
FbaI TGATCA 1 cut(s) 1117
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 2 cut(s) 233, 785
Fsp4HI GCNGC 1 cut(s) 100
GluI GCNGC 1 cut(s) 100
HaeIII GGCC 3 cut(s) 5, 102, 631
HapII CCGG 4 cut(s) 124, 539, 693, 719
Hin1II CATG 6 cut(s) 183, 271, 298, 1019, 1059, 1115
HincII GTYRAC 2 cut(s) 480, 576
HindII GTYRAC 2 cut(s) 480, 576
HinfI GANTC 5 cut(s) 109, 114, 715, 881, 895
HpaII CCGG 4 cut(s) 124, 539, 693, 719
HphI GGTGA 4 cut(s) 31, 202, 917, 971
Hpy166II GTNNAC 4 cut(s) 191, 480, 576, 1061
Hpy188I TCNGA 3 cut(s) 10, 57, 873
Hpy188III TCNNGA 6 cut(s) 225, 241, 311, 590, 815, 863
Hpy8I GTNNAC 4 cut(s) 191, 480, 576, 1061
Hpy99I CGWCG 1 cut(s) 472
HpyAV CCTTC 2 cut(s) 52, 228
HpyCH4III ACNGT 2 cut(s) 188, 637
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 8 cut(s) 183, 294, 298, 419, 518, 735, 1002, 1026
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 96, 808
HpyF3I CTNAG 7 cut(s) 7, 54, 128, 486, 870, 983, 1040
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 6 cut(s) 183, 271, 298, 1019, 1059, 1115
Ksp22I TGATCA 1 cut(s) 1117
Kzo9I GATC 2 cut(s) 273, 1117
LmnI GCTCC 1 cut(s) 96
LweI GCATC 2 cut(s) 255, 856
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 850
MalI GATC 2 cut(s) 275, 1119
MbiI CCGCTC 1 cut(s) 99
MboI GATC 2 cut(s) 273, 1117
MboII GAAGA 7 cut(s) 19, 22, 25, 28, 213, 904, 1067
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 2 cut(s) 389, 662
MluCI AATT 6 cut(s) 148, 445, 519, 702, 910, 917
MlyI GAGTC 2 cut(s) 889, 890
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 7 cut(s) 16, 224, 229, 507, 762, 852, 1089
MseI TTAA 1 cut(s) 1132
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 4 cut(s) 124, 539, 693, 719
MspR9I CCNGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 1004
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 59, 96, 808
NdeII GATC 2 cut(s) 273, 1117
NlaIII CATG 6 cut(s) 183, 271, 298, 1019, 1059, 1115
NlaIV GGNNCC 1 cut(s) 785
NspI RCATGY 2 cut(s) 298, 1059
PaeI GCATGC 1 cut(s) 298
PceI AGGCCT 1 cut(s) 631
PciI ACATGT 1 cut(s) 1055
PctI GAATGC 1 cut(s) 1004
PfeI GAWTC 3 cut(s) 109, 114, 715
PkrI GCNGC 1 cut(s) 101
PleI GAGTC 2 cut(s) 889, 889
PpsI GAGTC 2 cut(s) 889, 889
PscI ACATGT 1 cut(s) 1055
PshAI GACNNNNGTC 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 1 cut(s) 785
PspPI GGNCC 2 cut(s) 101, 784
RsaI GTAC 2 cut(s) 190, 965
RsaNI GTAC 2 cut(s) 189, 964
SaqAI TTAA 1 cut(s) 1132
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 2 cut(s) 273, 1117
Sau96I GGNCC 2 cut(s) 101, 784
SchI GAGTC 2 cut(s) 889, 890
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 2 cut(s) 389, 662
SfaNI GCATC 2 cut(s) 255, 856
SinI GGWCC 1 cut(s) 784
SmlI CTYRAG 1 cut(s) 843
SmoI CTYRAG 1 cut(s) 843
SphI GCATGC 1 cut(s) 298
Sse9I AATT 6 cut(s) 148, 445, 519, 702, 910, 917
SseBI AGGCCT 1 cut(s) 631
SsiI CCGC 6 cut(s) 99, 136, 143, 684, 857, 1109
StuI AGGCCT 1 cut(s) 631
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 433, 972
TaaI ACNGT 2 cut(s) 188, 637
TaiI ACGT 1 cut(s) 456
TaqI TCGA 7 cut(s) 112, 234, 240, 470, 947, 1069, 1081
TasI AATT 6 cut(s) 148, 445, 519, 702, 910, 917
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 3 cut(s) 109, 114, 715
Tru1I TTAA 1 cut(s) 1132
Tru9I TTAA 1 cut(s) 1132
TscAI CASTG 2 cut(s) 111, 262
TspDTI ATGAA 3 cut(s) 155, 168, 1004
TspRI CASTG 2 cut(s) 111, 262
VpaK11BI GGWCC 1 cut(s) 784
XagI CCTNNNNNAGG 1 cut(s) 222
XapI RAATTY 1 cut(s) 910
XceI RCATGY 2 cut(s) 298, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.