pycom06g10090

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000675
Physical Location & Seq
Reverse (-)
15457496 .. 15458879
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10090.2

Sequence Viewer

Length: 1113 bp
ATGGCTACCTTAGAAATCTTCACCATCTTATTCTTCTTGTTCCTCCTCCCTCATACAGCTACCACCTGTGGTATACCAAACTGTACAGCCGGCAGCCAACCAATTCGATTCCCGTTTCCGCTACGCAACTTTCAACTCTCAAATTGTGGGTATCCAGATTCATCGTTGAGAGCATCAACTACAGGCACCAAATCATATCCATCAACGACCCCAACAACTGTTTCCCTAGGGCGGTTTCTCGACCATGACATCAGTCTTAGAGATTCACCCTTCTTTTATCTGCATGGCCTCGAAAACTACACGTTCCTCGACTGCTCGTCTCAAGATGAAGCAGCATGGACTTACGCACCCATTTCTTGCCTCGGCAGTGAAATCTACAAGATCGTTGCAGTACCGACAAGTTTGTTATTGCCACCGGGTAACCAATCATCTAGGGGCAGGTGTTTTGTGATCTTAACAGCATCGGTTCCAAGTTTATTTGATCCATTGGAAAATGTTCAGTTGCAATGGGACAAGCCTGATTATCTTTCTTGCGAAGCAGTTGGTCTTTATTGTGGATTGAAGAGTGGTTCGCAAACAAAGTGCTTTGGTTCTTCAAATTCTTTTGGTGCCCCAAGATTTTTACTTGATGATGAAGATACGGTTACAATTTCAGTCTTTTCAGGTCTCTCAAGAATTGCGAAGTTTAGCATACTGGTAGGCGTGGGAATCCCAAGCTTTTGGTGTCTCATTGGCCTTGTGATTTATTTTTGTGATTTTATGAATGGTACAGTTCACCATCCTGCTACAGAACTATCCTCTGTAAACAGTCGACCACCTCCCATAATCGCAACGGGCCTTGATGGTCTGATCATCGAGTCATATCCTAAGACTCAGCTTGGTGAGAACTTGGAATTGCCGGAGCCGAAGGACAAGACTTGCTCCATATGTTTGGGTGAGTATAAGCCTAAAGCAACACTAAGGACCATCCCAGCATGCAACCATTATTTTTATGCTAGTTGCGTCGACGAGTGGCTTAGATTGAACGCGACGTGCCCTCTTTGTCGAAATGCACCGGACGATATTAAGTGCGTCTTAGACATGATTTTTCAAATGCCTGTCTTGTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

371

Amino Acids

40.74

Weight (kDa)

5.26

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 429
Acc36I ACCTGC 1 cut(s) 429
AccB1I GGYRCC 2 cut(s) 185, 608
AccI GTMKAC 3 cut(s) 73, 811, 1005
AccII CGCG 1 cut(s) 1028
AciI CCGC 2 cut(s) 119, 232
AclWI GGATC 1 cut(s) 476
AcsI RAATTY 1 cut(s) 598
AfaI GTAC 3 cut(s) 85, 393, 769
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 5 cut(s) 134, 562, 597, 1024, 1091
AhdI GACNNNNNGTC 1 cut(s) 316
AjiI CACGTC 1 cut(s) 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 205, 237
AluBI AGCT 3 cut(s) 59, 717, 877
AluI AGCT 3 cut(s) 59, 717, 877
Alw26I GTCTC 3 cut(s) 324, 671, 731
AlwI GGATC 1 cut(s) 476
AoxI GGCC 3 cut(s) 286, 733, 835
ApeKI GCWGC 2 cut(s) 93, 332
ApoI RAATTY 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 495
AspA2I CCTAGG 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 835, 963
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 5 cut(s) 13, 258, 767, 893, 947
AvaII GGWCC 1 cut(s) 963
AvrII CCTAGG 1 cut(s) 226
BaeGI GKGCMC 2 cut(s) 613, 1037
BanI GGYRCC 2 cut(s) 185, 608
BbvI GCAGC 2 cut(s) 105, 344
BccI CCATC 5 cut(s) 32, 208, 786, 836, 974
BciVI GTATCC 1 cut(s) 162
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 324, 671, 731
BfaI CTAG 3 cut(s) 227, 432, 996
BfmI CTRYAG 2 cut(s) 180, 786
BfuAI ACCTGC 1 cut(s) 429
BfuI GTATCC 1 cut(s) 162
BisI GCNGC 2 cut(s) 94, 333
BlnI CCTAGG 1 cut(s) 226
BlsI GCNGC 2 cut(s) 95, 334
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 1 cut(s) 963
BmeRI GACNNNNNGTC 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 1032
BmgT120I GGNCC 2 cut(s) 835, 963
BmiI GGNNCC 4 cut(s) 187, 468, 610, 903
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 182, 470
BoxI GACNNNNGTC 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 306, 655
BpuMI CCSGG 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 2 cut(s) 226, 361
BsaWI WCCGGW 1 cut(s) 1054
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 699
Bse3DI GCAATG 1 cut(s) 512
BseDI CCNNGG 2 cut(s) 226, 361
BseGI GGATG 2 cut(s) 778, 966
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 887
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 613, 1037
BseXI GCAGC 2 cut(s) 105, 344
BseYI CCCAGC 1 cut(s) 970
Bsh1236I CGCG 1 cut(s) 1028
BshFI GGCC 3 cut(s) 288, 735, 837
BshNI GGYRCC 2 cut(s) 185, 608
BsiSI CCGG 4 cut(s) 90, 416, 899, 1055
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 3 cut(s) 324, 671, 731
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 3 cut(s) 288, 735, 837
Bso31I GGTCTC 1 cut(s) 671
Bsp1286I GDGCHC 2 cut(s) 613, 1037
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 4 cut(s) 381, 450, 481, 849
BspACI CCGC 2 cut(s) 119, 232
BspANI GGCC 3 cut(s) 288, 735, 837
BspCNI CTCAG 1 cut(s) 886
BspFNI CGCG 1 cut(s) 1028
BspLI GGNNCC 4 cut(s) 187, 468, 610, 903
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 1 cut(s) 476
BspT107I GGYRCC 2 cut(s) 185, 608
BspTNI GGTCTC 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 1 cut(s) 699
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 226, 361
BssMI GATC 4 cut(s) 381, 450, 481, 849
BssNAI GTATAC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 226
Bst1107I GTATAC 1 cut(s) 74
Bst4CI ACNGT 5 cut(s) 83, 220, 643, 772, 809
Bst6I CTCTTC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 91, 976
BstDEI CTNAG 7 cut(s) 10, 257, 867, 873, 959, 1016, 1075
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 2 cut(s) 778, 966
BstFNI CGCG 1 cut(s) 1028
BstKTI GATC 4 cut(s) 384, 453, 484, 852
BstMAI GTCTC 3 cut(s) 324, 671, 731
BstMBI GATC 4 cut(s) 381, 450, 481, 849
BstNSI RCATGY 1 cut(s) 978
BstPAI GACNNNNGTC 1 cut(s) 252
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 2 cut(s) 180, 786
BstSLI GKGCMC 2 cut(s) 613, 1037
BstUI CGCG 1 cut(s) 1028
BstV1I GCAGC 2 cut(s) 105, 344
BstXI CCANNNNNNTGG 2 cut(s) 720, 931
BstZ17I GTATAC 1 cut(s) 74
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 3 cut(s) 288, 735, 837
BtrI CACGTC 1 cut(s) 1032
BtsCI GGATG 2 cut(s) 778, 966
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 373
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 2 cut(s) 91, 976
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 835, 963
CseI GACGC 2 cut(s) 991, 1060
Csp6I GTAC 3 cut(s) 84, 392, 768
CviAII CATG 5 cut(s) 245, 284, 336, 975, 1081
CviQI GTAC 3 cut(s) 84, 392, 768
DdeI CTNAG 7 cut(s) 10, 257, 867, 873, 959, 1016, 1075
DpnI GATC 4 cut(s) 383, 452, 483, 851
DpnII GATC 4 cut(s) 381, 450, 481, 849
DriI GACNNNNNGTC 1 cut(s) 316
Eam1104I CTCTTC 1 cut(s) 557
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 557
Eco130I CCWWGG 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 963
Eco91I GGTNACC 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 226
ErhI CCWWGG 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 5 cut(s) 248, 287, 339, 978, 1084
FalI AAGNNNNNCTT 2 cut(s) 1058, 1090
FaqI GGGAC 1 cut(s) 524
FatI CATG 5 cut(s) 244, 283, 335, 974, 1080
FauNDI CATATG 1 cut(s) 926
FbaI TGATCA 1 cut(s) 849
FblI GTMKAC 3 cut(s) 73, 811, 1005
Fnu4HI GCNGC 2 cut(s) 94, 333
FokI GGATG 2 cut(s) 765, 953
Fsp4HI GCNGC 2 cut(s) 94, 333
FspBI CTAG 3 cut(s) 227, 432, 996
GluI GCNGC 2 cut(s) 94, 333
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 3 cut(s) 288, 735, 837
HapII CCGG 4 cut(s) 90, 416, 899, 1055
HgaI GACGC 2 cut(s) 991, 1060
Hin1II CATG 5 cut(s) 248, 287, 339, 978, 1084
HincII GTYRAC 2 cut(s) 812, 1006
HindII GTYRAC 2 cut(s) 812, 1006
HindIII AAGCTT 1 cut(s) 715
HinfI GANTC 6 cut(s) 108, 158, 263, 708, 857, 871
HpaII CCGG 4 cut(s) 90, 416, 899, 1055
HphI GGTGA 5 cut(s) 13, 258, 767, 893, 947
Hpy166II GTNNAC 5 cut(s) 74, 775, 805, 812, 1006
Hpy188I TCNGA 1 cut(s) 849
Hpy188III TCNNGA 4 cut(s) 155, 239, 323, 672
Hpy8I GTNNAC 5 cut(s) 74, 775, 805, 812, 1006
Hpy99I CGWCG 3 cut(s) 1007, 1010, 1033
HpyAV CCTTC 2 cut(s) 280, 901
HpyCH4III ACNGT 5 cut(s) 83, 220, 643, 772, 809
HpyCH4IV ACGT 2 cut(s) 302, 1031
HpyCH4V TGCA 5 cut(s) 283, 389, 505, 978, 1052
HpyF3I CTNAG 7 cut(s) 10, 257, 867, 873, 959, 1016, 1075
HpySE526I ACGT 2 cut(s) 302, 1031
Hsp92II CATG 5 cut(s) 248, 287, 339, 978, 1084
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 849
Kzo9I GATC 4 cut(s) 381, 450, 481, 849
LmnI GCTCC 2 cut(s) 901, 926
Lsp1109I GCAGC 2 cut(s) 105, 344
LweI GCATC 2 cut(s) 182, 470
MaeI CTAG 3 cut(s) 227, 432, 996
MaeII ACGT 2 cut(s) 302, 1031
MaeIII GTNAC 2 cut(s) 419, 643
MalI GATC 4 cut(s) 383, 452, 483, 851
MboI GATC 4 cut(s) 381, 450, 481, 849
MboII GAAGA 5 cut(s) 10, 25, 574, 585, 647
MhlI GDGCHC 2 cut(s) 613, 1037
MluCI AATT 6 cut(s) 102, 142, 598, 648, 675, 893
MlyI GAGTC 2 cut(s) 865, 866
MnlI CCTC 9 cut(s) 53, 56, 60, 299, 317, 371, 808, 828, 1047
MroNI GCCGGC 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 3 cut(s) 455, 1065, 1111
MspI CCGG 4 cut(s) 90, 416, 899, 1055
MspR9I CCNGG 1 cut(s) 417
MvnI CGCG 1 cut(s) 1028
NaeI GCCGGC 1 cut(s) 91
NciI CCSGG 1 cut(s) 417
NdeI CATATG 1 cut(s) 926
NdeII GATC 4 cut(s) 381, 450, 481, 849
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 5 cut(s) 248, 287, 339, 978, 1084
NlaIV GGNNCC 4 cut(s) 187, 468, 610, 903
NmeAIII GCCGAG 1 cut(s) 342
NspI RCATGY 1 cut(s) 978
PaeI GCATGC 1 cut(s) 978
PaqCI CACCTGC 1 cut(s) 429
PdiI GCCGGC 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 4 cut(s) 108, 158, 263, 708
PkrI GCNGC 2 cut(s) 95, 334
PleI GAGTC 2 cut(s) 865, 865
PpsI GAGTC 2 cut(s) 865, 865
PshAI GACNNNNGTC 1 cut(s) 252
PspEI GGTNACC 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 970
PspN4I GGNNCC 4 cut(s) 187, 468, 610, 903
PspPI GGNCC 2 cut(s) 835, 963
RsaI GTAC 3 cut(s) 85, 393, 769
RsaNI GTAC 3 cut(s) 84, 392, 768
SalI GTCGAC 2 cut(s) 810, 1004
SaqAI TTAA 3 cut(s) 455, 1065, 1111
SatI GCNGC 2 cut(s) 94, 333
Sau3AI GATC 4 cut(s) 381, 450, 481, 849
Sau96I GGNCC 2 cut(s) 835, 963
SchI GAGTC 2 cut(s) 865, 866
ScrFI CCNGG 1 cut(s) 417
SduI GDGCHC 2 cut(s) 613, 1037
SfaNI GCATC 2 cut(s) 182, 470
SfcI CTRYAG 2 cut(s) 180, 786
SgrDI CGTCGACG 1 cut(s) 1004
SinI GGWCC 1 cut(s) 963
SmlI CTYRAG 2 cut(s) 321, 670
SmoI CTYRAG 2 cut(s) 321, 670
SphI GCATGC 1 cut(s) 978
Sse9I AATT 6 cut(s) 102, 142, 598, 648, 675, 893
SsiI CCGC 2 cut(s) 119, 232
SspMI CTAG 3 cut(s) 227, 432, 996
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 5 cut(s) 83, 220, 643, 772, 809
TaiI ACGT 2 cut(s) 305, 1034
TaqI TCGA 8 cut(s) 106, 240, 291, 309, 811, 855, 1005, 1045
TasI AATT 6 cut(s) 102, 142, 598, 648, 675, 893
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 4 cut(s) 108, 158, 263, 708
Tru1I TTAA 3 cut(s) 455, 1065, 1111
Tru9I TTAA 3 cut(s) 455, 1065, 1111
TscAI CASTG 1 cut(s) 373
TseI GCWGC 2 cut(s) 93, 332
TspDTI ATGAA 4 cut(s) 150, 342, 648, 776
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 963
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 978
XmaJI CCTAGG 1 cut(s) 226
XmiI GTMKAC 3 cut(s) 73, 811, 1005
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 227, 432, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.