RLG00000004159

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000675
Physical Location & Seq
Forward (+)
56752876 .. 56754156
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004159

Sequence Viewer

Length: 1152 bp
ATGGCAACCGTAATCACATTACTGCTATTGTTCTTCTCCTTCTTCTTCTTCTTCTTCCTTCCTCACATAGCAGCTACAACCTGCGGCGCATCAGCATGCGCTGCCAGCGGCCCGTCAGTTCGATTCCCATTCCGGCTAACTGATCGCCAGCAAACGGGTTGTGGGTATTCCGGTAAGTTTGATGTATCGTGCAACGGACACAAACAAACCATTCTTAACCTCCCATCTTCCGGAAACTTCACGGTTGAGAGCGTCAGTTACAGGGACCAACTCGTGTGGATCAACGATCGAGACAACTGCATCTTCAAGCGGTTTCTTGACAAGGACTTCAGTCTCATAGGTTCACCCTTCCGGTACGCGCTTGACCTTCAAGAGTACACCTTCTACAAATGTTCGCCCCAACTTGTGCCACTGTGGTCTCCCATCTCCTGTCTCAGCAGTGACAATTATGAGGTCATTGGCGTCGCGAGCTGGTCAACGATATCGAGATCACCTTCTCCCTATTGTGATGTGATTACCAGAGCTTTGGTTCCAGCAGAAAGTAATGAGTTAGGGAATTCTGACTTTGGTGTGGGGCTGACTTGGAAGGAGCCTGATTGTCGTTCTTGTGAAGCGAGTGGTAAGGTTTGTGGGTTTCAGAATGGTTCACAGTCGAAAATTCACTGCTCTAGTACTCTATCTTATGGTCTATCAAAAGCTACGAAGTATGGCATAGCGATCGGCGTATTAATTCCAGGGCTATTATCCATTACCTGTTGTGCACTTTATGTCTCAGCCCCGAGAAGGGATCATGGTCGAATCCAAGAATCTACCACCGAATTATCCGGTACTGTAACTAATCGTCAACCTCAAGTATTCATAGCAGGCCTTGATGGTCAGACAATTGAGTCATATCCCAAGACTCAACTCGGCGAGAGTTTGGAATTGCCTGACCCTAATGACAAAACTTGTCCAATATGTCTGGGTGAGTACCAACCGAAGGAAACTCTGAGGACTATACCAGAATGTAACCATTATTTCCATGCTACTTGCATTGACGAATGGCTTAGAAAAAATCCGACATGCCCGCTTTGTCGCAATCTACCCGGTCTTTGTCGAAATCCACTGGAGGTGTCATCAGTGAGACCACGTGATGATTATGCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

384

Amino Acids

42.44

Weight (kDa)

6.32

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 96 2.4e-14 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 314 - 359 1.2e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 316 - 359 1.3e-14 Ring finger domain
zf-C3HC4_2 PF13923 317 - 358 4.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 317 - 345 4.1e-09 RING-like zinc finger
zf-C3HC4 PF00097 317 - 358 2e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 886
Acc36I ACCTGC 1 cut(s) 89
AccII CGCG 2 cut(s) 359, 467
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 4 cut(s) 84, 108, 310, 1067
AclWI GGATC 2 cut(s) 287, 795
AcsI RAATTY 2 cut(s) 556, 657
AcuI CTGAAG 1 cut(s) 313
AcvI CACGTG 1 cut(s) 1130
AcyI GRCGYC 1 cut(s) 462
AfaI GTAC 5 cut(s) 356, 377, 673, 829, 971
AfiI CCNNNNNNNGG 5 cut(s) 154, 230, 783, 784, 979
AgsI TTSAA 2 cut(s) 307, 371
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 4 cut(s) 74, 471, 524, 698
AluI AGCT 4 cut(s) 74, 471, 524, 698
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 6 cut(s) 285, 338, 423, 437, 775, 1117
Alw44I GTGCAC 1 cut(s) 759
AlwI GGATC 2 cut(s) 287, 795
Ama87I CYCGRG 1 cut(s) 778
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 109, 865
ApaLI GTGCAC 1 cut(s) 759
ApeKI GCWGC 2 cut(s) 71, 101
ApoI RAATTY 2 cut(s) 556, 657
AseI ATTAAT 1 cut(s) 728
AspLEI GCGC 3 cut(s) 89, 101, 361
AspS9I GGNCC 2 cut(s) 110, 265
AsuC2I CCSGG 1 cut(s) 1086
AsuHPI GGTGA 3 cut(s) 336, 483, 977
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 1 cut(s) 265
BaeGI GKGCMC 1 cut(s) 763
BauI CACGAG 1 cut(s) 272
BbrPI CACGTG 1 cut(s) 1130
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 2 cut(s) 83, 88
BccI CCATC 3 cut(s) 232, 431, 866
BcgI CGANNNNNNTGC 4 cut(s) 279, 313, 700, 734
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 1 cut(s) 1086
BcoDI GTCTC 6 cut(s) 285, 338, 423, 437, 775, 1117
BfaI CTAG 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 89
BisI GCNGC 4 cut(s) 72, 85, 102, 109
BlsI GCNGC 4 cut(s) 73, 86, 103, 110
BmcAI AGTACT 1 cut(s) 673
Bme1390I CCNGG 2 cut(s) 735, 1086
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 110, 265
BmiI GGNNCC 3 cut(s) 266, 531, 591
BmrFI CCNGG 2 cut(s) 735, 1086
BmsI GCATC 2 cut(s) 98, 309
BoxI GACNNNNGTC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 1127
BpuEI CTTGAG 1 cut(s) 834
BpuMI CCSGG 1 cut(s) 1086
BsaAI YACGTR 1 cut(s) 1130
BsaHI GRCGYC 1 cut(s) 462
BsaI GGTCTC 2 cut(s) 423, 1117
BsaJI CCNNGG 1 cut(s) 734
BsaWI WCCGGW 4 cut(s) 170, 230, 351, 824
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 230, 783, 784, 979
Bse1I ACTGG 1 cut(s) 1110
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 1 cut(s) 734
BseLI CCNNNNNNNGG 5 cut(s) 154, 230, 783, 784, 979
BseMII CTCAG 3 cut(s) 448, 786, 980
BseNI ACTGG 1 cut(s) 1110
BseSI GKGCMC 1 cut(s) 763
BseXI GCAGC 2 cut(s) 83, 88
Bsh1236I CGCG 2 cut(s) 359, 467
Bsh1285I CGRYCG 2 cut(s) 289, 720
BshFI GGCC 2 cut(s) 111, 867
BsiEI CGRYCG 2 cut(s) 289, 720
BsiHKAI GWGCWC 1 cut(s) 763
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 6 cut(s) 133, 171, 231, 352, 825, 1086
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 5 cut(s) 154, 230, 783, 784, 979
BsmAI GTCTC 6 cut(s) 285, 338, 423, 437, 775, 1117
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 111, 867
Bso31I GGTCTC 2 cut(s) 423, 1117
BsoBI CYCGRG 1 cut(s) 778
Bsp1286I GDGCHC 1 cut(s) 763
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 6 cut(s) 142, 279, 286, 488, 717, 787
Bsp68I TCGCGA 1 cut(s) 467
BspACI CCGC 4 cut(s) 84, 108, 310, 1067
BspANI GGCC 2 cut(s) 111, 867
BspCNI CTCAG 3 cut(s) 447, 785, 981
BspEI TCCGGA 1 cut(s) 230
BspFNI CGCG 2 cut(s) 359, 467
BspLI GGNNCC 3 cut(s) 266, 531, 591
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 2 cut(s) 287, 795
BspTNI GGTCTC 2 cut(s) 423, 1117
BsrI ACTGG 1 cut(s) 1110
BssECI CCNNGG 1 cut(s) 734
BssMI GATC 6 cut(s) 142, 279, 286, 488, 717, 787
BssNI GRCGYC 1 cut(s) 462
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 5 cut(s) 10, 244, 414, 651, 832
BstACI GRCGYC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 101
BstBAI YACGTR 1 cut(s) 1130
BstC8I GCNNGC 6 cut(s) 97, 106, 149, 469, 865, 1067
BstDEI CTNAG 4 cut(s) 434, 772, 989, 1046
BstFNI CGCG 2 cut(s) 359, 467
BstHHI GCGC 3 cut(s) 89, 101, 361
BstKTI GATC 6 cut(s) 145, 282, 289, 491, 720, 790
BstMAI GTCTC 6 cut(s) 285, 338, 423, 437, 775, 1117
BstMBI GATC 6 cut(s) 142, 279, 286, 488, 717, 787
BstMCI CGRYCG 2 cut(s) 289, 720
BstMWI GCNNNNNNNGC 3 cut(s) 101, 105, 468
BstNI CCWGG 1 cut(s) 735
BstNSI RCATGY 2 cut(s) 99, 1065
BstPAI GACNNNNGTC 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 733, 1084
BstSLI GKGCMC 1 cut(s) 763
BstUI CGCG 2 cut(s) 359, 467
BstV1I GCAGC 2 cut(s) 83, 88
BstXI CCANNNNNNTGG 1 cut(s) 526
BsuRI GGCC 2 cut(s) 111, 867
BtsI GCAGTG 2 cut(s) 445, 661
BtsIMutI CAGTG 5 cut(s) 410, 445, 661, 1103, 1125
BtuMI TCGCGA 1 cut(s) 467
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 6 cut(s) 97, 106, 149, 469, 865, 1067
CfoI GCGC 3 cut(s) 89, 101, 361
Cfr13I GGNCC 2 cut(s) 110, 265
CseI GACGC 2 cut(s) 241, 451
Csp6I GTAC 5 cut(s) 355, 376, 672, 828, 970
CspCI CAANNNNNGTGG 2 cut(s) 257, 292
CviAII CATG 4 cut(s) 96, 791, 1022, 1062
CviQI GTAC 5 cut(s) 355, 376, 672, 828, 970
DdeI CTNAG 4 cut(s) 434, 772, 989, 1046
DpnI GATC 6 cut(s) 144, 281, 288, 490, 719, 789
DpnII GATC 6 cut(s) 142, 279, 286, 488, 717, 787
DrdI GACNNNNNNGTC 1 cut(s) 886
DseDI GACNNNNNNGTC 1 cut(s) 886
Eco147I AGGCCT 1 cut(s) 867
Eco31I GGTCTC 2 cut(s) 423, 1117
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 313
Eco72I CACGTG 1 cut(s) 1130
Eco88I CYCGRG 1 cut(s) 778
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 483
FaeI CATG 4 cut(s) 99, 794, 1025, 1065
FaqI GGGAC 1 cut(s) 278
FatI CATG 4 cut(s) 95, 790, 1021, 1061
FauI CCCGC 1 cut(s) 1074
Fnu4HI GCNGC 4 cut(s) 72, 85, 102, 109
Fsp4HI GCNGC 4 cut(s) 72, 85, 102, 109
FspBI CTAG 1 cut(s) 669
GlaI GCGC 3 cut(s) 88, 100, 360
GluI GCNGC 4 cut(s) 72, 85, 102, 109
GsuI CTGGAG 1 cut(s) 1127
HaeIII GGCC 2 cut(s) 111, 867
HapII CCGG 6 cut(s) 133, 171, 231, 352, 825, 1086
HgaI GACGC 2 cut(s) 241, 451
HhaI GCGC 3 cut(s) 89, 101, 361
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 4 cut(s) 99, 794, 1025, 1065
Hin6I GCGC 3 cut(s) 87, 99, 359
HinP1I GCGC 3 cut(s) 87, 99, 359
HincII GTYRAC 2 cut(s) 477, 845
HindII GTYRAC 2 cut(s) 477, 845
HinfI GANTC 5 cut(s) 123, 798, 806, 887, 901
HpaII CCGG 6 cut(s) 133, 171, 231, 352, 825, 1086
HphI GGTGA 3 cut(s) 336, 483, 977
Hpy166II GTNNAC 6 cut(s) 344, 378, 477, 647, 761, 845
Hpy188I TCNGA 5 cut(s) 562, 639, 879, 990, 1059
Hpy188III TCNNGA 6 cut(s) 231, 290, 317, 371, 466, 486
Hpy8I GTNNAC 6 cut(s) 344, 378, 477, 647, 761, 845
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 9 cut(s) 49, 68, 358, 377, 391, 504, 580, 777, 973
HpyCH4III ACNGT 5 cut(s) 10, 244, 414, 651, 832
HpyCH4IV ACGT 1 cut(s) 1129
HpyCH4V TGCA 4 cut(s) 192, 300, 761, 1032
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 105, 468
HpyF3I CTNAG 4 cut(s) 434, 772, 989, 1046
HpySE526I ACGT 1 cut(s) 1129
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 4 cut(s) 99, 794, 1025, 1065
HspAI GCGC 3 cut(s) 87, 99, 359
Kpn2I TCCGGA 1 cut(s) 230
Kzo9I GATC 6 cut(s) 142, 279, 286, 488, 717, 787
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 2 cut(s) 83, 88
LweI GCATC 2 cut(s) 98, 309
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 1129
MaeIII GTNAC 4 cut(s) 257, 440, 832, 1007
MalI GATC 6 cut(s) 144, 281, 288, 490, 719, 789
MboI GATC 6 cut(s) 142, 279, 286, 488, 717, 787
MboII GAAGA 8 cut(s) 25, 34, 37, 40, 43, 46, 219, 295
MfeI CAATTG 1 cut(s) 882
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 7 cut(s) 445, 556, 657, 729, 818, 882, 923
MlyI GAGTC 2 cut(s) 895, 896
MmeI TCCRAC 1 cut(s) 1082
MnlI CCTC 6 cut(s) 72, 230, 445, 858, 984, 1102
MroI TCCGGA 1 cut(s) 230
MseI TTAA 3 cut(s) 216, 728, 1150
MslI CAYNNNNRTG 1 cut(s) 94
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 6 cut(s) 133, 171, 231, 352, 825, 1086
MspR9I CCNGG 2 cut(s) 735, 1086
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 2 cut(s) 359, 467
MwoI GCNNNNNNNGC 3 cut(s) 101, 105, 468
NciI CCSGG 1 cut(s) 1086
NdeII GATC 6 cut(s) 142, 279, 286, 488, 717, 787
NlaIII CATG 4 cut(s) 99, 794, 1025, 1065
NlaIV GGNNCC 3 cut(s) 266, 531, 591
NmeAIII GCCGAG 1 cut(s) 888
NmuCI GTSAC 1 cut(s) 440
NruI TCGCGA 1 cut(s) 467
NspI RCATGY 2 cut(s) 99, 1065
PaeI GCATGC 1 cut(s) 99
PceI AGGCCT 1 cut(s) 867
PfeI GAWTC 3 cut(s) 123, 798, 806
PkrI GCNGC 4 cut(s) 73, 86, 103, 110
Ple19I CGATCG 2 cut(s) 289, 720
PleI GAGTC 2 cut(s) 895, 895
PmaCI CACGTG 1 cut(s) 1130
PmlI CACGTG 1 cut(s) 1130
PpsI GAGTC 2 cut(s) 895, 895
Ppu21I YACGTR 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 728
Psp6I CCWGG 1 cut(s) 733
PspCI CACGTG 1 cut(s) 1130
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 3 cut(s) 266, 531, 591
PspPI GGNCC 2 cut(s) 110, 265
PvuI CGATCG 2 cut(s) 289, 720
RruI TCGCGA 1 cut(s) 467
RsaI GTAC 5 cut(s) 356, 377, 673, 829, 971
RsaNI GTAC 5 cut(s) 355, 376, 672, 828, 970
RseI CAYNNNNRTG 1 cut(s) 94
SaqAI TTAA 3 cut(s) 216, 728, 1150
SatI GCNGC 4 cut(s) 72, 85, 102, 109
Sau3AI GATC 6 cut(s) 142, 279, 286, 488, 717, 787
Sau96I GGNCC 2 cut(s) 110, 265
ScaI AGTACT 1 cut(s) 673
SchI GAGTC 2 cut(s) 895, 896
ScrFI CCNGG 2 cut(s) 735, 1086
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 2 cut(s) 98, 309
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 94
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
SphI GCATGC 1 cut(s) 99
Sse9I AATT 7 cut(s) 445, 556, 657, 729, 818, 882, 923
SseBI AGGCCT 1 cut(s) 867
SsiI CCGC 4 cut(s) 84, 108, 310, 1067
SspMI CTAG 1 cut(s) 669
StuI AGGCCT 1 cut(s) 867
StyD4I CCNGG 2 cut(s) 733, 1084
TaaI ACNGT 5 cut(s) 10, 244, 414, 651, 832
TaiI ACGT 1 cut(s) 1132
TaqI TCGA 6 cut(s) 121, 289, 485, 653, 796, 1096
TasI AATT 7 cut(s) 445, 556, 657, 729, 818, 882, 923
TatI WGTACW 2 cut(s) 375, 671
TauI GCSGC 2 cut(s) 87, 111
TfiI GAWTC 3 cut(s) 123, 798, 806
Tru1I TTAA 3 cut(s) 216, 728, 1150
Tru9I TTAA 3 cut(s) 216, 728, 1150
TscAI CASTG 5 cut(s) 417, 445, 668, 1110, 1125
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 71, 101
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 1 cut(s) 847
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 5 cut(s) 417, 445, 668, 1110, 1125
VneI GTGCAC 1 cut(s) 759
VpaK11BI GGWCC 1 cut(s) 265
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 2 cut(s) 556, 657
XceI RCATGY 2 cut(s) 99, 1065
XspI CTAG 1 cut(s) 669
ZrmI AGTACT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.