FvH4_5g04550

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000675
Physical Location & Seq
Reverse (-)
2677082 .. 2682262
5181 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04550.t1

Sequence Viewer

Length: 3306 bp
ATGGCAACCATAATAACATTACTGCCATTCTTCTTCTGTTTCTTCTTCCTTCCTCAATTAGCAGCTAACTCATGCTATGTATCACCGTGCGCTGCCGGGGGCCAACCAATTCGATTCCCTTTCCGGCTAATTGATCAGCAACCTCCATCTTGTGGGTATTCTACTGATTTTGATCTCTCGTGCAACGAACTTAAACAAACCATTTTCACTCTCCCATCTTCCGGAGACTTCATAGTCGAGAGCATCAGTTATACAGACCAATTCATGCGGATCAACGACGGCGACAACTGCACCATCAAGCGGTTCCTTGACAAGAAATTCACTCTTCTAGGCTCGCCTTTTCAGTTCTCGCTTGACCTTCAAGAGTACACATTCTACAAGTGCAAGCCCCAACGTGTGCCACTGTGGTGGTATCCCATATCTTGCCTCAGCAATGACAACTACGAGGTTGTTGGTGTCGTGAGCCGGTCAACTTCGACAATACCTTCCGGCTTCTGTGATGTGATTACCACAGCTATGGTTCCAGCAGAAAGTTTTTATGAGTCGACGAACTCTGGTGTGAAGCTGACTTGGAACGTGCCTGATTGTGGTTCTTGCGAATTAAGTGGTAACGTTTGCGGGTTTCAGAATGGTTCACGTTCAAAAATTCACTGCTCTAGTAAAACATCTTATGGTCTCTCAAAAGCTGCAAAATATGGCATAGCTGTTGGCGTGTTAATTCCAGGGCTATTATCCATTACCTGTTGTGCACTTTATGTTTCATCACAAAGGGGGCAGCGGCCGGAGCGCAATCGTGGTCGGCTCCATGAAATCACCACAGAATTATCCGGTACTGTATTTAATCGTCACCCTCAAGTACTGTTCATTCCCGGCCTTGATAGTTTGACAATTGCGTCATATCCCAAGACTCAACTTGGTGAGAGTTTGGAATTGCCCAACCCTAATGACAAAACTTGTCCAATATGTCTGGGGGAATACCAACCGAAGGAAACTCTGAGGACTATACCAGAATGCAACCATTATTTCCATTCTAATTGCATAGACGAGTGGCTTGGAAAAAATCCGACATGTCCCCTTTGTCGCAAACTACCCGCTTTTTGCCGGAATCCACCGGAGCTCGATCTTATGCCTACCTTAGAAATCATCATCACCTTCTTCATCTTCTTGTTTTCCCTTTCTCCAAATCAAACATCAGCAACCTGTCATGAAACTAGATGCAAGGCCAAGGAACAATGGGTACGATTCCCTTTTCAACTAATAAGAGAAGGCCAAGACTTGGCTTGTGGCTATGATCGAGGGTTTGATCTCTCCTGCAGCAACCACCACAAGCGAACCATCCTCACTCTCCCATCTTCCGGAGACTTCATCGTGAAGGACATCAACTACATCGACCATATCATATGGATCGACGATCCCGAAAGCTGCTTCCCTAAGAAGCTCATGGATCATAACTTCAGTCTTGTAGGTTCACCCTTCTCATACTTTTACGGCCTTGAAAGCTACTTATTCCTCAACTGTCCGTCCGGAGAAGATATACTAGCACCGTCTTCACCAATTGATTGCCTCAGTAGTGATGAATACAAGGTCTTTGCAGTGCAATCTACTTGGTTCTATGCCGGTCTTAATACATCAAGTGTTTCATCGAGGCCACTGGCTCAGACATCGTTATCACGTCTTTGTTCCGTGATCTCTACTGCTTTGATTCCACTGTCAGGTTCATTTGACGGGTGGGATCTTAACTTTGGTGTTCGGTTGGCATGGAACCTGCCCGATTGTCGTCTATGCGAAGCAAGCGGGAGATTATGTGGGCTTGATATTGCCACAGGTTCTCAAGATGTGTGCTCTGGACCTTCAAATTCAGTACACCACGGCAATTCAAGAGGCGAGACGCTCACAACTGCAACGTATGTCATAGGCGGGGGAATACTTGTGCTGTTGTTCGTCTGCGTGCTTCCAATGTGTTGTTCTTCTAACCCACCTAGCGATGAACCCACCACACTGACCACAGGATGGTCGGGAGTAACAAATCATCAACAACCTCCTCTAGCATTGGATCCGGAGCTCAACTGTGTGACTACTGAAGCATATCCCAAGACTCAACTTGGAGATGAGAGTTTGGAACTGCCTAACCCTAATGACAAAACTTGCGCCATATGTTTGTGCGACTACCAACCAAAGGAAACAATAAAAACTATACCCCTATGTAACCATTACTTTCATATAGCTGCTACCGGATGTGGTGGTTCATCATGCTATCGGGGCGGTCCATCGATTCGATTCCCTTTCCGGCTAAGATACAACCAACCATTAAATTGTGGTTATTCCGTTCACTTTGACCTCTCATGCGACGTACAACAACGCACTATTCTCACCCTCCCATCTTCAGGAGACTTCGAGGTCGAGAGCATCAATTACAGTGACCAAACCTTCTGGATCAACGACCTCAACTGGTGCATGCACAAACCGTTCCTTGACAAAGACTTCAGCCTTGTTGGCACGCCCTTCCATTTCTGGCATCGCCTGGAAAACTATACCTACTTCAAGTGCCCGTCCGAAGTTGTGTCTATTGGGCCTTTTGCATCAATCTTTTGCCTCGGCGGTTCCGACTACAACGTCATTGCCGTCCCGTCAAGTTGGTCAACTACTCAGAAACCACCTCCTCAGTCCCACTGTCGTGCAATTTCCACAGCTTTGCTTCCGTCCGGGAGCTTTGACGCCTTTGAGTGGGGGGAGTTCTTTGCTGTAAAGTTGACTTGGAACGTTCCCGATTGTAGTTCTTGTGAAGCAACTGGTAGATTTTGTGGGTTTAATGTTACAAGTTCGCAAATTGTGTGCTCCAGTTCAAATCATGGTGTCTCAAGAGCTACAAAGAAAAGCATAGTATTTGGCGTGTTGATTAGTGTTGCAGTGCTGGTGTGTATCGTTGGATACGGACTCCATGTTTCCGGCAGGAGGAGGGATGAGGTTCGGGTCGACCAAGAAACAAATGGGCGTGTTCGGCTTCAAGAACTCGTCGCAGAATTATCCACCGTAGTCACAACCGGGCTTGATGCTCAGACTATTGAGTCATATCAGAAGACTCAACTTGGTGAGAGTTTGGATTTACCTGACCCTAATGACAAGACTTGCTCGATATGTTTGGGTGAGTACCAGCCTAAGGAAACGGTAAGGACTATACCGGAATGTAATCATTATTTTCATGTTGGTTGCATAGACGAGTGGCTTAGAAAGAACCCTACATGTCCACTTTGTCGGAATCTACCTCGAACACCCGAAGTACCCCTGGTGAGACAGCATGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

1102

Amino Acids

122.37

Weight (kDa)

5.67

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 30 - 93 2.1e-14 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 315 - 361 1.7e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 318 - 361 1.3e-13 Ring finger domain
zf-RING_11 PF17123 319 - 347 8.8e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 319 - 360 1.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 319 - 360 6.2e-08 Zinc finger, C3HC4 type (RING finger)
GUB_WAK_bind PF13947 401 - 471 8.3e-13 Wall-associated receptor kinase galacturonan-binding
GUB_WAK_bind PF13947 746 - 814 1.2e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 1041 - 1085 7.8e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 1042 - 1085 7.5e-14 Ring finger domain
zf-RING_11 PF17123 1043 - 1071 4.6e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 1043 - 1084 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 1043 - 1084 2.1e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 5 cut(s) 233, 892, 2396, 2612, 3064
Acc36I ACCTGC 1 cut(s) 1773
AccB7I CCANNNNNTGG 3 cut(s) 152, 1276, 2010
AccI GTMKAC 2 cut(s) 545, 2973
AccIII TCCGGA 4 cut(s) 221, 1355, 1523, 2056
AciI CCGC 9 cut(s) 268, 301, 618, 778, 1092, 1794, 1917, 2262, 2598
AclI AACGTT 2 cut(s) 612, 2760
AclWI GGATC 8 cut(s) 278, 1406, 1412, 1452, 1740, 2048, 2061, 2441
AcoI YGGCCR 1 cut(s) 779
AcsI RAATTY 3 cut(s) 317, 645, 1855
AcuI CTGAAG 4 cut(s) 1438, 2100, 2367, 2467
AcyI GRCGYC 1 cut(s) 2715
AfaI GTAC 8 cut(s) 368, 832, 858, 1239, 1863, 2352, 3149, 3279
AflIII ACRYGT 3 cut(s) 394, 1067, 3239
AgsI TTSAA 9 cut(s) 362, 642, 1253, 1496, 1854, 1878, 2542, 2844, 3005
AjiI CACGTC 1 cut(s) 1673
AjnI CCWGG 3 cut(s) 721, 2520, 3282
AjuI GAANNNNNNNTTGG 2 cut(s) 1948, 1980
AleI CACNNNNGTG 2 cut(s) 406, 2673
Alw21I GWGCWC 5 cut(s) 751, 1119, 1844, 2064, 2837
Alw26I GTCTC 7 cut(s) 219, 680, 1353, 1880, 2382, 2860, 3283
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 8 cut(s) 278, 1406, 1412, 1452, 1740, 2048, 2061, 2441
Aor13HI TCCGGA 4 cut(s) 221, 1355, 1523, 2056
AoxI GGCC 8 cut(s) 100, 779, 871, 1221, 1267, 1489, 1646, 2570
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 7 cut(s) 62, 92, 686, 775, 1314, 1422, 2225
ApoI RAATTY 3 cut(s) 317, 645, 1855
AspLEI GCGC 3 cut(s) 92, 789, 2150
AspS9I GGNCC 4 cut(s) 100, 1847, 2264, 2570
AsuC2I CCSGG 4 cut(s) 97, 870, 2704, 3043
AvaII GGWCC 2 cut(s) 1847, 2264
AxyI CCTNAGG 1 cut(s) 3156
BaeGI GKGCMC 2 cut(s) 751, 2549
BamHI GGATCC 1 cut(s) 2053
BanII GRGCYC 2 cut(s) 1119, 2064
BauI CACGAG 1 cut(s) 178
BbsI GAAGAC 2 cut(s) 1539, 3083
Bbv12I GWGCWC 5 cut(s) 751, 1119, 1844, 2064, 2837
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 7 cut(s) 74, 79, 673, 787, 1326, 1409, 2212
BccI CCATC 8 cut(s) 154, 223, 302, 1343, 1357, 2004, 2275, 2386
BceAI ACGGC 4 cut(s) 295, 1504, 1885, 2606
BciT130I CCWGG 3 cut(s) 723, 2522, 3284
BciVI GTATCC 2 cut(s) 423, 2921
BclI TGATCA 1 cut(s) 133
BcnI CCSGG 4 cut(s) 97, 870, 2704, 3043
BcoDI GTCTC 7 cut(s) 219, 680, 1353, 1880, 2382, 2860, 3283
BfaI CTAG 6 cut(s) 329, 657, 1212, 1538, 1980, 2045
BfmI CTRYAG 1 cut(s) 1312
BfuAI ACCTGC 1 cut(s) 1773
BfuI GTATCC 2 cut(s) 423, 2921
BglI GCCNNNNNGGC 1 cut(s) 2493
BisI GCNGC 8 cut(s) 63, 93, 687, 776, 779, 1315, 1423, 2226
BlsI GCNGC 8 cut(s) 64, 94, 688, 777, 780, 1316, 1424, 2227
BmcAI AGTACT 1 cut(s) 858
Bme1390I CCNGG 7 cut(s) 97, 723, 870, 2522, 2704, 3043, 3284
Bme18I GGWCC 2 cut(s) 1847, 2264
BmgBI CACGTC 1 cut(s) 1673
BmgT120I GGNCC 4 cut(s) 100, 1847, 2264, 2570
BmiI GGNNCC 7 cut(s) 101, 305, 522, 803, 1763, 2055, 2602
BmrFI CCNGG 7 cut(s) 97, 723, 870, 2522, 2704, 3043, 3284
BmsI GCATC 6 cut(s) 252, 1205, 2415, 2524, 2588, 3040
BpiI GAAGAC 2 cut(s) 1539, 3083
BpmI CTGGAG 1 cut(s) 2821
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 3 cut(s) 837, 1815, 2842
BpuMI CCSGG 4 cut(s) 97, 870, 2704, 3043
Bsa29I ATCGAT 2 cut(s) 2270, 3301
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 2715
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 6 cut(s) 96, 722, 1224, 1867, 2593, 3282
BsaWI WCCGGW 8 cut(s) 221, 827, 1111, 1355, 1523, 2056, 2231, 3178
BsaXI ACNNNNNCTCC 2 cut(s) 1789, 1819
Bse118I RCCGGY 2 cut(s) 465, 1616
Bse1I ACTGG 4 cut(s) 1656, 2453, 2794, 2838
Bse21I CCTNAGG 1 cut(s) 3156
Bse3DI GCAATG 2 cut(s) 439, 2616
Bse8I GATNNNNATC 1 cut(s) 171
BseAI TCCGGA 4 cut(s) 221, 1355, 1523, 2056
BseBI CCWGG 3 cut(s) 723, 2522, 3284
BseCI ATCGAT 2 cut(s) 2270, 3301
BseDI CCNNGG 6 cut(s) 96, 722, 1224, 1867, 2593, 3282
BseGI GGATG 4 cut(s) 1335, 2015, 2240, 2965
BseJI GATNNNNATC 1 cut(s) 171
BseMI GCAATG 2 cut(s) 439, 2616
BseMII CTCAG 7 cut(s) 442, 986, 1579, 1670, 2660, 2675, 3068
BseNI ACTGG 4 cut(s) 1656, 2453, 2794, 2838
BseRI GAGGAG 3 cut(s) 2031, 2649, 2968
BseSI GKGCMC 2 cut(s) 751, 2549
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 7 cut(s) 74, 79, 673, 787, 1326, 1409, 2212
BsgI GTGCAG 1 cut(s) 274
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 8 cut(s) 102, 781, 873, 1223, 1269, 1491, 1648, 2572
BshVI ATCGAT 2 cut(s) 2270, 3301
BsiEI CGRYCG 1 cut(s) 782
BsiHKAI GWGCWC 5 cut(s) 751, 1119, 1844, 2064, 2837
BslFI GGGAC 3 cut(s) 1056, 2609, 2650
BsmAI GTCTC 7 cut(s) 219, 680, 1353, 1880, 2382, 2860, 3283
BsmBI CGTCTC 1 cut(s) 1880
BsmFI GGGAC 3 cut(s) 1056, 2609, 2650
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 8 cut(s) 102, 781, 873, 1223, 1269, 1491, 1648, 2572
Bso31I GGTCTC 1 cut(s) 680
Bsp1286I GDGCHC 6 cut(s) 751, 1119, 1844, 2064, 2549, 2837
Bsp13I TCCGGA 4 cut(s) 221, 1355, 1523, 2056
BspACI CCGC 9 cut(s) 268, 301, 618, 778, 1092, 1794, 1917, 2262, 2598
BspANI GGCC 8 cut(s) 102, 781, 873, 1223, 1269, 1491, 1648, 2572
BspCNI CTCAG 7 cut(s) 441, 987, 1578, 1669, 2659, 2674, 3067
BspDI ATCGAT 2 cut(s) 2270, 3301
BspEI TCCGGA 4 cut(s) 221, 1355, 1523, 2056
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 7 cut(s) 101, 305, 522, 803, 1763, 2055, 2602
BspMAI CTGCAG 1 cut(s) 1316
BspMI ACCTGC 1 cut(s) 1773
BspPI GGATC 8 cut(s) 278, 1406, 1412, 1452, 1740, 2048, 2061, 2441
BspTNI GGTCTC 1 cut(s) 680
BsrDI GCAATG 2 cut(s) 439, 2616
BsrFI RCCGGY 2 cut(s) 465, 1616
BsrI ACTGG 4 cut(s) 1656, 2453, 2794, 2838
BssAI RCCGGY 2 cut(s) 465, 1616
BssECI CCNNGG 6 cut(s) 96, 722, 1224, 1867, 2593, 3282
BssNI GRCGYC 1 cut(s) 2715
BssSI CACGAG 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 1224
Bst2BI CACGAG 1 cut(s) 178
Bst2UI CCWGG 3 cut(s) 723, 2522, 3284
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 2715
BstC8I GCNNGC 6 cut(s) 335, 386, 1792, 1949, 2456, 2498
BstDSI CCRYGG 1 cut(s) 1867
BstF5I GGATG 4 cut(s) 1335, 2015, 2240, 2965
BstHHI GCGC 3 cut(s) 92, 789, 2150
BstMAI GTCTC 7 cut(s) 219, 680, 1353, 1880, 2382, 2860, 3283
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 7 cut(s) 288, 784, 1497, 1791, 2259, 2493, 2998
BstNI CCWGG 3 cut(s) 723, 2522, 3284
BstNSI RCATGY 3 cut(s) 1071, 2458, 3243
BstSCI CCNGG 7 cut(s) 95, 721, 868, 2520, 2702, 3041, 3282
BstSFI CTRYAG 1 cut(s) 1312
BstSLI GKGCMC 2 cut(s) 751, 2549
BstV1I GCAGC 7 cut(s) 74, 79, 673, 787, 1326, 1409, 2212
BstV2I GAAGAC 2 cut(s) 1539, 3083
BstX2I RGATCY 2 cut(s) 1732, 2053
BstXI CCANNNNNNTGG 2 cut(s) 408, 517
BstYI RGATCY 2 cut(s) 1732, 2053
BstZI CGGCCG 1 cut(s) 779
Bsu15I ATCGAT 2 cut(s) 2270, 3301
Bsu36I CCTNAGG 1 cut(s) 3156
BsuI GTATCC 2 cut(s) 423, 2921
BsuRI GGCC 8 cut(s) 102, 781, 873, 1223, 1269, 1491, 1648, 2572
BsuTUI ATCGAT 2 cut(s) 2270, 3301
BtgI CCRYGG 1 cut(s) 1867
BtgZI GCGATG 2 cut(s) 1998, 2501
BtrI CACGTC 1 cut(s) 1673
BtsCI GGATG 4 cut(s) 1335, 2015, 2240, 2965
BtsI GCAGTG 3 cut(s) 649, 1599, 2913
BtsIMutI CAGTG 9 cut(s) 401, 649, 1599, 1649, 1706, 1997, 2422, 2668, 2913
BveI ACCTGC 1 cut(s) 1773
Cac8I GCNNGC 6 cut(s) 335, 386, 1792, 1949, 2456, 2498
CciI TCATGA 1 cut(s) 1204
CfoI GCGC 3 cut(s) 92, 789, 2150
Cfr10I RCCGGY 2 cut(s) 465, 1616
Cfr13I GGNCC 4 cut(s) 100, 1847, 2264, 2570
ClaI ATCGAT 2 cut(s) 2270, 3301
CseI GACGC 3 cut(s) 882, 1897, 2723
Csp6I GTAC 8 cut(s) 367, 831, 857, 1238, 1862, 2351, 3148, 3278
CviQI GTAC 8 cut(s) 367, 831, 857, 1238, 1862, 2351, 3148, 3278
DrdI GACNNNNNNGTC 5 cut(s) 233, 892, 2396, 2612, 3064
DseDI GACNNNNNNGTC 5 cut(s) 233, 892, 2396, 2612, 3064
EaeI YGGCCR 1 cut(s) 779
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 2 cut(s) 1117, 2062
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 1 cut(s) 1224
Eco24I GRGCYC 2 cut(s) 1119, 2064
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 1847, 2264
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 2 cut(s) 1117, 2062
Eco57I CTGAAG 4 cut(s) 1438, 2100, 2367, 2467
Eco81I CCTNAGG 1 cut(s) 3156
EcoICRI GAGCTC 2 cut(s) 1117, 2062
EcoRII CCWGG 3 cut(s) 721, 2520, 3282
EcoT14I CCWWGG 1 cut(s) 1224
EcoT38I GRGCYC 2 cut(s) 1119, 2064
ErhI CCWWGG 1 cut(s) 1224
Esp3I CGTCTC 1 cut(s) 1880
FaqI GGGAC 3 cut(s) 1056, 2609, 2650
FauI CCCGC 4 cut(s) 611, 1099, 1787, 1910
FauNDI CATATG 2 cut(s) 1400, 2153
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 545, 2973
Fnu4HI GCNGC 8 cut(s) 63, 93, 687, 776, 779, 1315, 1423, 2226
FokI GGATG 4 cut(s) 1322, 2022, 2247, 2972
FriOI GRGCYC 2 cut(s) 1119, 2064
Fsp4HI GCNGC 8 cut(s) 63, 93, 687, 776, 779, 1315, 1423, 2226
FspBI CTAG 6 cut(s) 329, 657, 1212, 1538, 1980, 2045
GlaI GCGC 3 cut(s) 91, 788, 2149
GluI GCNGC 8 cut(s) 63, 93, 687, 776, 779, 1315, 1423, 2226
GsuI CTGGAG 1 cut(s) 2821
HaeIII GGCC 8 cut(s) 102, 781, 873, 1223, 1269, 1491, 1648, 2572
HgaI GACGC 3 cut(s) 882, 1897, 2723
HhaI GCGC 3 cut(s) 92, 789, 2150
Hin1I GRCGYC 1 cut(s) 2715
Hin6I GCGC 3 cut(s) 90, 787, 2148
HinP1I GCGC 3 cut(s) 90, 787, 2148
HincII GTYRAC 5 cut(s) 471, 546, 2640, 2751, 2974
HindII GTYRAC 5 cut(s) 471, 546, 2640, 2751, 2974
Hpy99I CGWCG 5 cut(s) 281, 550, 1412, 2351, 3017
HpyCH4IV ACGT 9 cut(s) 394, 576, 612, 637, 1672, 1904, 2349, 2613, 2760
HpyF10VI GCNNNNNNNGC 7 cut(s) 288, 784, 1497, 1791, 2259, 2493, 2998
HpySE526I ACGT 9 cut(s) 394, 576, 612, 637, 1672, 1904, 2349, 2613, 2760
Hsp92I GRCGYC 1 cut(s) 2715
HspAI GCGC 3 cut(s) 90, 787, 2148
Kpn2I TCCGGA 4 cut(s) 221, 1355, 1523, 2056
Ksp22I TGATCA 1 cut(s) 133
LmnI GCTCC 6 cut(s) 784, 807, 1114, 2059, 2706, 2840
Lsp1109I GCAGC 7 cut(s) 74, 79, 673, 787, 1326, 1409, 2212
LweI GCATC 6 cut(s) 252, 1205, 2415, 2524, 2588, 3040
MaeI CTAG 6 cut(s) 329, 657, 1212, 1538, 1980, 2045
MaeII ACGT 9 cut(s) 394, 576, 612, 637, 1672, 1904, 2349, 2613, 2760
MaeIII GTNAC 8 cut(s) 608, 845, 2020, 2071, 2204, 2417, 2812, 3034
MfeI CAATTG 2 cut(s) 888, 1554
MflI RGATCY 2 cut(s) 1732, 2053
MhlI GDGCHC 6 cut(s) 751, 1119, 1844, 2064, 2549, 2837
MlyI GAGTC 6 cut(s) 551, 901, 2089, 2928, 3073, 3074
MmeI TCCRAC 4 cut(s) 1088, 2628, 2905, 3233
MroI TCCGGA 4 cut(s) 221, 1355, 1523, 2056
MseI TTAA 8 cut(s) 192, 602, 716, 840, 1623, 1737, 2309, 2808
MslI CAYNNNNRTG 3 cut(s) 406, 515, 2673
MspA1I CMGCKG 1 cut(s) 778
MspR9I CCNGG 7 cut(s) 97, 723, 870, 2522, 2704, 3043, 3284
MunI CAATTG 2 cut(s) 888, 1554
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 3 cut(s) 723, 2522, 3284
MwoI GCNNNNNNNGC 7 cut(s) 288, 784, 1497, 1791, 2259, 2493, 2998
NciI CCSGG 4 cut(s) 97, 870, 2704, 3043
NdeI CATATG 2 cut(s) 1400, 2153
NlaIV GGNNCC 7 cut(s) 101, 305, 522, 803, 1763, 2055, 2602
NmeAIII GCCGAG 1 cut(s) 2574
NmuCI GTSAC 4 cut(s) 845, 2071, 2417, 3034
NspI RCATGY 3 cut(s) 1071, 2458, 3243
OliI CACNNNNGTG 2 cut(s) 406, 2673
PaeI GCATGC 1 cut(s) 2458
PagI TCATGA 1 cut(s) 1204
PciI ACATGT 2 cut(s) 1067, 3239
PcsI WCGNNNNNNNCGW 3 cut(s) 1413, 2601, 2619
PctI GAATGC 1 cut(s) 1016
PfeI GAWTC 7 cut(s) 114, 1105, 1242, 1702, 2272, 2277, 3256
PflMI CCANNNNNTGG 3 cut(s) 152, 1276, 2010
PfoI TCCNGGA 1 cut(s) 2702
PkrI GCNGC 8 cut(s) 64, 94, 688, 777, 780, 1316, 1424, 2227
PleI GAGTC 6 cut(s) 550, 901, 2089, 2928, 3073, 3073
PpsI GAGTC 6 cut(s) 550, 901, 2089, 2928, 3073, 3073
PscI ACATGT 2 cut(s) 1067, 3239
Psp124BI GAGCTC 2 cut(s) 1119, 2064
Psp1406I AACGTT 2 cut(s) 612, 2760
Psp6I CCWGG 3 cut(s) 721, 2520, 3282
PspGI CCWGG 3 cut(s) 721, 2520, 3282
PspN4I GGNNCC 7 cut(s) 101, 305, 522, 803, 1763, 2055, 2602
PspPI GGNCC 4 cut(s) 100, 1847, 2264, 2570
PsrI GAACNNNNNNTAC 2 cut(s) 1221, 1253
PstI CTGCAG 1 cut(s) 1316
PsuI RGATCY 2 cut(s) 1732, 2053
RsaI GTAC 8 cut(s) 368, 832, 858, 1239, 1863, 2352, 3149, 3279
RsaNI GTAC 8 cut(s) 367, 831, 857, 1238, 1862, 2351, 3148, 3278
RseI CAYNNNNRTG 3 cut(s) 406, 515, 2673
SacI GAGCTC 2 cut(s) 1119, 2064
SalI GTCGAC 2 cut(s) 544, 2972
SaqAI TTAA 8 cut(s) 192, 602, 716, 840, 1623, 1737, 2309, 2808
SatI GCNGC 8 cut(s) 63, 93, 687, 776, 779, 1315, 1423, 2226
Sau96I GGNCC 4 cut(s) 100, 1847, 2264, 2570
ScaI AGTACT 1 cut(s) 858
SchI GAGTC 6 cut(s) 551, 901, 2089, 2928, 3073, 3074
ScrFI CCNGG 7 cut(s) 97, 723, 870, 2522, 2704, 3043, 3284
SduI GDGCHC 6 cut(s) 751, 1119, 1844, 2064, 2549, 2837
SfaNI GCATC 6 cut(s) 252, 1205, 2415, 2524, 2588, 3040
SfcI CTRYAG 1 cut(s) 1312
SinI GGWCC 2 cut(s) 1847, 2264
SmiMI CAYNNNNRTG 3 cut(s) 406, 515, 2673
SmlI CTYRAG 3 cut(s) 852, 1830, 2857
SmoI CTYRAG 3 cut(s) 852, 1830, 2857
SphI GCATGC 1 cut(s) 2458
SsiI CCGC 9 cut(s) 268, 301, 618, 778, 1092, 1794, 1917, 2262, 2598
SspMI CTAG 6 cut(s) 329, 657, 1212, 1538, 1980, 2045
SstI GAGCTC 2 cut(s) 1119, 2064
StyD4I CCNGG 7 cut(s) 95, 721, 868, 2520, 2702, 3041, 3282
StyI CCWWGG 1 cut(s) 1224
TaiI ACGT 9 cut(s) 397, 579, 615, 640, 1675, 1907, 2352, 2616, 2763
TatI WGTACW 3 cut(s) 366, 856, 1861
TauI GCSGC 1 cut(s) 781
TfiI GAWTC 7 cut(s) 114, 1105, 1242, 1702, 2272, 2277, 3256
Tru1I TTAA 8 cut(s) 192, 602, 716, 840, 1623, 1737, 2309, 2808
Tru9I TTAA 8 cut(s) 192, 602, 716, 840, 1623, 1737, 2309, 2808
TscAI CASTG 9 cut(s) 408, 656, 1599, 1656, 1713, 2004, 2422, 2675, 2913
TseFI GTSAC 4 cut(s) 845, 2071, 2417, 3034
TseI GCWGC 7 cut(s) 62, 92, 686, 775, 1314, 1422, 2225
Tsp45I GTSAC 4 cut(s) 845, 2071, 2417, 3034
TspGWI ACGGA 5 cut(s) 1509, 1672, 2314, 2688, 2946
TspRI CASTG 9 cut(s) 408, 656, 1599, 1656, 1713, 2004, 2422, 2675, 2913
Van91I CCANNNNNTGG 3 cut(s) 152, 1276, 2010
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 1847, 2264
XapI RAATTY 3 cut(s) 317, 645, 1855
XceI RCATGY 3 cut(s) 1071, 2458, 3243
XcmI CCANNNNNNNNNTGG 1 cut(s) 2984
XmiI GTMKAC 2 cut(s) 545, 2973
XspI CTAG 6 cut(s) 329, 657, 1212, 1538, 1980, 2045
ZrmI AGTACT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.