FvH4_5g29040

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000675
Physical Location & Seq
Forward (+)
20152103 .. 20153533
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29040.t1

Sequence Viewer

Length: 1290 bp
ATGCCTACCGTAGAAGTCTTCATCACCTTGCTCTTCTTCTCCTTCTTCGTCTTCCTCCTTCCTAATCAGATATCAGCAAGCACTACTAACTGTCGTAAATCAAAATGTATGAGAAACGAACCACCCGTGCATTTCCCGTTTCGGCTAGGAGACCGCCAAGCCACATATTGTGGTTATGGTCAGGGCTTCAATCTCTCATGCAGCTTGGAACAACGAACCATCCTCAGCCTCTCGTATTCAGGTGACTTCATTGTGCGGGGCATCGACTACGATGACCAGCTCGTGTGGATCGACGACCCCGAAAACTGCTTCCCTAAGCGGCTGCTCGACGGTGACTTTAGTCTTATGGACTCTCCCTTCACGTACTTGTACGGCCTTGAAAACTTTACCTTCCTCAATTGCTCCTCGCAAGCAGAAATTCGGTCTTCACCCATACCTTGCCTCAGTGATCAGTACTATGGTGTCCTTGTTGTTCCTTCCAGCTGGTTCTATGGTGATCCCAATTCAACATCAACGACATCATCACTTTCTCCGGCATCGCTGTCACCTCTTTGCTCTGTGATTTCCACAGCCTTGGTTCCAAGGTCAAGTCCAGCTGACCCGTGGGATATTAACATCGGAGTTCCGCTGGCGTGGGACATGCCTGAATGTCGTTCTTGTCAAGGAAGCAGTTGTGGATTCGAGAATTCCACAAGTTCGCAAATTGTGTGCACTGGTTCAGGTATGTCAAACGCTCTAAGGTATGCAATCGTGATAGGCTGTGCAGTACCAGCGCTAGTGTGCATCATCGGGGTCATAATTTGTGCTTACAAGATGAATGCTTCTAATAATCAAGCTCGCCAACCGGCCGTAACAGAATTATCAACCGTAGTAAGCCAACAACTTCCGAATGCAACCGCCGTCGGCCCTCCTACTGCAGTTAGAATGGGACTGGATGGTGAGACAATTGAATCATATCCCAAGACTCAACTAGGTGAGAGTTTGGAGTTACCCAACCCTAATGACAAAACTTGTTCGATATGTTTGTGTGAGTACCAACATAGAGAAATAATAAGGACTATACCTCAATGCAAGCATTATTTTCATGCTAATTGCATAGACGAGTGGTTCAAAAAGGATGTAACTTGCCCCCTCTGCCGAGATCTGCCCAATTATGAAGCGCTTGCATTGCCTAGACCGCCCTTGCATGATACGAGTGCTCTACCAAGTAACGGATCACTTGATTTGCCTAGACCCGTCTTGCAAAATACTGCTAATTTTCTGTATCTCGATCATATGTATTTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

430

Amino Acids

47.43

Weight (kDa)

4.89

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 99 2.9e-14 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 337 - 380 9.4e-12 Ring finger domain
zf-RING_11 PF17123 338 - 366 1e-05 RING-like zinc finger
zf-rbx1 PF12678 342 - 380 1e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 154, 256, 319, 626, 897, 1178
AclWI GGATC 3 cut(s) 296, 491, 1222
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 2 cut(s) 417, 685
AfaI GTAC 5 cut(s) 365, 371, 455, 768, 1034
AfeI AGCGCT 2 cut(s) 774, 1161
AfiI CCNNNNNNNGG 1 cut(s) 1211
AgsI TTSAA 5 cut(s) 190, 380, 507, 950, 1111
AluBI AGCT 5 cut(s) 204, 280, 483, 596, 836
AluI AGCT 5 cut(s) 204, 280, 483, 596, 836
Alw21I GWGCWC 2 cut(s) 713, 1201
Alw26I GTCTC 2 cut(s) 144, 935
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 3 cut(s) 296, 491, 1222
Aor51HI AGCGCT 2 cut(s) 774, 1161
AoxI GGCC 3 cut(s) 373, 846, 904
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 201, 322
ApoI RAATTY 2 cut(s) 417, 685
AspLEI GCGC 2 cut(s) 775, 1162
AspS9I GGNCC 1 cut(s) 905
AsuHPI GGTGA 8 cut(s) 16, 254, 344, 420, 506, 537, 950, 986
BaeGI GKGCMC 1 cut(s) 713
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 3 cut(s) 10, 43, 417
Bbv12I GWGCWC 2 cut(s) 713, 1201
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 2 cut(s) 213, 309
BccI CCATC 2 cut(s) 227, 929
BceAI ACGGC 3 cut(s) 388, 833, 884
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 144, 935
BfaI CTAG 5 cut(s) 146, 776, 971, 1173, 1230
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 2 cut(s) 776, 1163
BglII AGATCT 1 cut(s) 1141
BisI GCNGC 3 cut(s) 202, 320, 323
BlsI GCNGC 3 cut(s) 203, 321, 324
BmcAI AGTACT 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 1 cut(s) 579
BmsI GCATC 3 cut(s) 270, 545, 792
BoxI GACNNNNGTC 1 cut(s) 339
BpiI GAAGAC 3 cut(s) 10, 43, 417
Bpu10I CCTNAGC 2 cut(s) 224, 315
BsaAI YACGTR 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 573, 581, 602
Bsc4I CCNNNNNNNGG 1 cut(s) 1211
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 2 cut(s) 718, 936
Bse3DI GCAATG 1 cut(s) 1166
BseDI CCNNGG 3 cut(s) 573, 581, 602
BseGI GGATG 3 cut(s) 219, 940, 1123
BseLI CCNNNNNNNGG 1 cut(s) 1211
BseMI GCAATG 1 cut(s) 1166
BseMII CTCAG 2 cut(s) 238, 457
BseNI ACTGG 2 cut(s) 718, 936
BseRI GAGGAG 1 cut(s) 394
BseSI GKGCMC 1 cut(s) 713
BseX3I CGGCCG 1 cut(s) 846
BseXI GCAGC 2 cut(s) 213, 309
BsgI GTGCAG 1 cut(s) 783
Bsh1285I CGRYCG 1 cut(s) 849
BshFI GGCC 3 cut(s) 375, 848, 906
BsiEI CGRYCG 1 cut(s) 849
BsiHKAI GWGCWC 2 cut(s) 713, 1201
BsiSI CCGG 2 cut(s) 533, 845
BslFI GGGAC 2 cut(s) 650, 942
BslI CCNNNNNNNGG 1 cut(s) 1211
BsmAI GTCTC 2 cut(s) 144, 935
BsmFI GGGAC 2 cut(s) 650, 942
BsmI GAATGC 2 cut(s) 823, 895
BsnI GGCC 3 cut(s) 375, 848, 906
Bso31I GGTCTC 1 cut(s) 144
Bsp1286I GDGCHC 2 cut(s) 713, 1201
Bsp143I GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
BspACI CCGC 6 cut(s) 154, 256, 319, 626, 897, 1178
BspANI GGCC 3 cut(s) 375, 848, 906
BspCNI CTCAG 2 cut(s) 237, 456
BspLI GGNNCC 1 cut(s) 579
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 3 cut(s) 296, 491, 1222
BspQI GCTCTTC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 144
BsrDI GCAATG 1 cut(s) 1166
BsrFI RCCGGY 1 cut(s) 844
BsrI ACTGG 2 cut(s) 718, 936
BssAI RCCGGY 1 cut(s) 844
BssECI CCNNGG 3 cut(s) 573, 581, 602
BssMI GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 573, 581
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 4 cut(s) 10, 92, 332, 868
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 1 cut(s) 363
BstC8I GCNNGC 6 cut(s) 79, 411, 630, 838, 1073, 1164
BstDEI CTNAG 4 cut(s) 224, 315, 443, 737
BstDSI CCRYGG 1 cut(s) 602
BstF5I GGATG 3 cut(s) 219, 940, 1123
BstH2I RGCGCY 2 cut(s) 776, 1163
BstHHI GCGC 2 cut(s) 775, 1162
BstKTI GATC 6 cut(s) 291, 451, 499, 1144, 1217, 1273
BstMAI GTCTC 2 cut(s) 144, 935
BstMBI GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
BstMCI CGRYCG 1 cut(s) 849
BstMWI GCNNNNNNNGC 4 cut(s) 770, 1134, 1168, 1177
BstNSI RCATGY 1 cut(s) 643
BstPAI GACNNNNGTC 1 cut(s) 339
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 713
BstV1I GCAGC 2 cut(s) 213, 309
BstV2I GAAGAC 3 cut(s) 10, 43, 417
BstX2I RGATCY 1 cut(s) 1141
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 1 cut(s) 1141
BstZI CGGCCG 1 cut(s) 846
BsuRI GGCC 3 cut(s) 375, 848, 906
BtgI CCRYGG 1 cut(s) 602
BtgZI GCGATG 1 cut(s) 522
BtsCI GGATG 3 cut(s) 219, 940, 1123
BtsIMutI CAGTG 2 cut(s) 451, 711
Cac8I GCNNGC 6 cut(s) 79, 411, 630, 838, 1073, 1164
CfoI GCGC 2 cut(s) 775, 1162
Cfr10I RCCGGY 1 cut(s) 844
Cfr13I GGNCC 1 cut(s) 905
Csp6I GTAC 5 cut(s) 364, 370, 454, 767, 1033
CviAII CATG 4 cut(s) 198, 640, 1085, 1187
CviQI GTAC 5 cut(s) 364, 370, 454, 767, 1033
DdeI CTNAG 4 cut(s) 224, 315, 443, 737
DpnI GATC 6 cut(s) 290, 450, 498, 1143, 1216, 1272
DpnII GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
EaeI YGGCCR 1 cut(s) 846
EagI CGGCCG 1 cut(s) 846
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
EclXI CGGCCG 1 cut(s) 846
Eco130I CCWWGG 2 cut(s) 573, 581
Eco31I GGTCTC 1 cut(s) 144
Eco32I GATATC 1 cut(s) 72
Eco47III AGCGCT 2 cut(s) 774, 1161
Eco52I CGGCCG 1 cut(s) 846
EcoRI GAATTC 1 cut(s) 685
EcoRV GATATC 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 573, 581
ErhI CCWWGG 2 cut(s) 573, 581
FaeI CATG 4 cut(s) 201, 643, 1088, 1190
FaqI GGGAC 2 cut(s) 650, 942
FatI CATG 4 cut(s) 197, 639, 1084, 1186
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 1275
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 202, 320, 323
FokI GGATG 3 cut(s) 206, 947, 1130
Fsp4HI GCNGC 3 cut(s) 202, 320, 323
FspBI CTAG 5 cut(s) 146, 776, 971, 1173, 1230
GlaI GCGC 2 cut(s) 774, 1161
GluI GCNGC 3 cut(s) 202, 320, 323
HaeII RGCGCY 2 cut(s) 776, 1163
HaeIII GGCC 3 cut(s) 375, 848, 906
HapII CCGG 2 cut(s) 533, 845
HhaI GCGC 2 cut(s) 775, 1162
Hin1II CATG 4 cut(s) 201, 643, 1088, 1190
Hin6I GCGC 2 cut(s) 773, 1160
HinP1I GCGC 2 cut(s) 773, 1160
HinfI GANTC 4 cut(s) 350, 678, 950, 964
HpaII CCGG 2 cut(s) 533, 845
HphI GGTGA 8 cut(s) 16, 254, 344, 420, 506, 537, 950, 986
Hpy166II GTNNAC 1 cut(s) 711
Hpy188I TCNGA 3 cut(s) 69, 620, 888
Hpy188III TCNNGA 3 cut(s) 682, 751, 1268
Hpy8I GTNNAC 1 cut(s) 711
Hpy99I CGWCG 3 cut(s) 296, 332, 905
HpyAV CCTTC 5 cut(s) 52, 68, 367, 400, 486
HpyCH4III ACNGT 4 cut(s) 10, 92, 332, 868
HpyCH4IV ACGT 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 4 cut(s) 770, 1134, 1168, 1177
HpyF3I CTNAG 4 cut(s) 224, 315, 443, 737
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 4 cut(s) 201, 643, 1088, 1190
HspAI GCGC 2 cut(s) 773, 1160
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 2 cut(s) 213, 309
LweI GCATC 3 cut(s) 270, 545, 792
MaeI CTAG 5 cut(s) 146, 776, 971, 1173, 1230
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 7 cut(s) 242, 332, 543, 850, 987, 1120, 1208
MalI GATC 6 cut(s) 290, 450, 498, 1143, 1216, 1272
MboI GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
MboII GAAGA 6 cut(s) 10, 25, 28, 37, 43, 417
MfeI CAATTG 2 cut(s) 397, 945
MflI RGATCY 1 cut(s) 1141
MhlI GDGCHC 2 cut(s) 713, 1201
MlyI GAGTC 2 cut(s) 344, 958
MseI TTAA 1 cut(s) 612
MspA1I CMGCKG 3 cut(s) 483, 596, 628
MspI CCGG 2 cut(s) 533, 845
MunI CAATTG 2 cut(s) 397, 945
Mva1269I GAATGC 2 cut(s) 823, 895
MwoI GCNNNNNNNGC 4 cut(s) 770, 1134, 1168, 1177
NdeI CATATG 1 cut(s) 1275
NdeII GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
NlaIII CATG 4 cut(s) 201, 643, 1088, 1190
NlaIV GGNNCC 1 cut(s) 579
NmeAIII GCCGAG 1 cut(s) 1163
NmuCI GTSAC 3 cut(s) 242, 332, 543
NspI RCATGY 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 38
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 288, 297
PctI GAATGC 2 cut(s) 823, 895
PfeI GAWTC 2 cut(s) 678, 950
PkrI GCNGC 3 cut(s) 203, 321, 324
PleI GAGTC 2 cut(s) 344, 958
PpsI GAGTC 2 cut(s) 344, 958
Ppu21I YACGTR 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 1 cut(s) 905
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 1141
PvuII CAGCTG 2 cut(s) 483, 596
RsaI GTAC 5 cut(s) 365, 371, 455, 768, 1034
RsaNI GTAC 5 cut(s) 364, 370, 454, 767, 1033
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 3 cut(s) 202, 320, 323
Sau3AI GATC 6 cut(s) 288, 448, 496, 1141, 1214, 1270
Sau96I GGNCC 1 cut(s) 905
ScaI AGTACT 1 cut(s) 455
SchI GAGTC 2 cut(s) 344, 958
SduI GDGCHC 2 cut(s) 713, 1201
SfaNI GCATC 3 cut(s) 270, 545, 792
SfcI CTRYAG 1 cut(s) 915
SsiI CCGC 6 cut(s) 154, 256, 319, 626, 897, 1178
SspMI CTAG 5 cut(s) 146, 776, 971, 1173, 1230
StyI CCWWGG 2 cut(s) 573, 581
TaaI ACNGT 4 cut(s) 10, 92, 332, 868
TaiI ACGT 1 cut(s) 365
TaqI TCGA 6 cut(s) 264, 291, 327, 681, 1016, 1269
TaqII GACCGA 1 cut(s) 411
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 2 cut(s) 678, 950
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 2 cut(s) 451, 718
TseFI GTSAC 3 cut(s) 242, 332, 543
TseI GCWGC 2 cut(s) 201, 322
Tsp45I GTSAC 3 cut(s) 242, 332, 543
TspDTI ATGAA 5 cut(s) 10, 238, 830, 1073, 1170
TspGWI ACGGA 1 cut(s) 1227
TspRI CASTG 2 cut(s) 451, 718
VneI GTGCAC 1 cut(s) 709
XapI RAATTY 2 cut(s) 417, 685
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 600
XspI CTAG 5 cut(s) 146, 776, 971, 1173, 1230
ZrmI AGTACT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.