MD14G1128600.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000675
Physical Location & Seq
Reverse (-)
20545245 .. 20546803
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1128600.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGCTACCATTGAAATCTTCACATTCTTCTTATTCTTATTCCTCTCCTTCCATCCTCAAAGCGCTGCCACCTGCGGTCAATCGAAATGCTCCTACGAGGGCCCGGTGGTTCGTTTCCCTTTCAGCTGGATAGGTTACCAACAAGCCTCAAAAAATTGTGGGTGGCCAGGGTTTTCTCTCTCATGCAACAGCAACAGTCAAACCATCATCACTCTGCAATCTTCTAGGGAATTCATTGTCCAAAACATCGACTACTTGTACCAAGTTGTAACGATCAACGATGGGGATAACTGCCTCCCTAAACGGCTTCTCAACAACGACCTCGATCTTGTAAATTCACCCTTCTATTACACGAACAGCCCTCTAAACTACACATTCCTAAACTGCTCGTTCTATTATAAAATTTGGTCTGCGCCCTTGATCTCTTGCCTTAGCAGTGAGACATACAAGGTCATTGTTGTGCCGACGAGCTTCTTATCGCCACCTGATCACGAACCGTACCGTCGTTATTGTGTTCCAATGTCGACAACTTTGGTTCCAAGCTTATTTGATTGGCTAGATCTTGACCTAGGAGCTCAGTTGACATGGTCTATGCCTGATTGTCGTTATTGTGAAGCAAGGGGTCTTGCTTGTGGGGCCAATAATGGCAGCATTTCCAGCCCTAGGTGCTTTGGTCCGTCGGAACCCGCTCTCTCAACAGTTGTAAGGTATTGCATAGCGATGGCCGCAGTAATTTTAGGGATATTGAGCATTGGGGCACTTGCAGTTTATATTTTTGACAAAAAAAGGGCTCGTGGTGAGCATCGCCAACCCAATATGGAATTATCTATCATCACCTATCAACAACCTTCTGTCGAGTTATTTGGTCTTGATCGTCCGACCATCGAATCGTATCCCAAGATTCAACTTGGTGAGAGGTCGGAATTTCCAAAGCAACTCGTTGATGACAATACTTGCCCTATATGTTTGGGTGAGTACCAGCCAAAGGAAACCCTGAGGACTATACCACAATGCAACCATTATTTTCACGCTAATTGCATAGATGAGTGGCTTAGAAGGAATGCAACGTGCCCGCTTTGTCGACTTCCACCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

366

Amino Acids

41.35

Weight (kDa)

6.22

Isoelectric Point (pI)

56.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 94 2.3e-14 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 317 - 361 1.9e-14 Ring finger domain
zf-rbx1 PF12678 317 - 361 2.7e-09 RING-H2 zinc finger domain
zf-RING_11 PF17123 319 - 347 2.6e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 319 - 360 3.7e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 319 - 360 1.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 319 - 358 1.8e-06 RING-type zinc-finger
zf-RING_5 PF14634 319 - 361 4.6e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AarI CACCTGC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 689
AccI GTMKAC 2 cut(s) 524, 1081
AciI CCGC 4 cut(s) 75, 687, 726, 1073
AcoI YGGCCR 2 cut(s) 164, 723
AcsI RAATTY 4 cut(s) 230, 334, 402, 923
AfaI GTAC 3 cut(s) 260, 500, 977
AfeI AGCGCT 1 cut(s) 64
AfiI CCNNNNNNNGG 2 cut(s) 663, 985
AgsI TTSAA 2 cut(s) 14, 905
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 4 cut(s) 126, 471, 543, 575
AluI AGCT 4 cut(s) 126, 471, 543, 575
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 64
AoxI GGCC 4 cut(s) 100, 164, 636, 723
ApaI GGGCCC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 65, 648
ApoI RAATTY 4 cut(s) 230, 334, 402, 923
AspA2I CCTAGG 2 cut(s) 568, 662
AspLEI GCGC 2 cut(s) 65, 415
AspS9I GGNCC 4 cut(s) 100, 101, 636, 674
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 5 cut(s) 330, 809, 826, 923, 983
AvaII GGWCC 1 cut(s) 674
AvrII CCTAGG 2 cut(s) 568, 662
AxyI CCTNAGG 1 cut(s) 995
BaeGI GKGCMC 3 cut(s) 104, 760, 1073
BalI TGGCCA 1 cut(s) 166
BanII GRGCYC 3 cut(s) 104, 577, 793
BauI CACGAG 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 52, 660
BccI CCATC 5 cut(s) 60, 212, 275, 715, 890
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 1 cut(s) 903
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 4 cut(s) 225, 557, 569, 663
BfoI RGCGCY 1 cut(s) 66
BfuAI ACCTGC 1 cut(s) 80
BfuI GTATCC 1 cut(s) 903
BglII AGATCT 1 cut(s) 559
BisI GCNGC 3 cut(s) 66, 649, 726
BlnI CCTAGG 2 cut(s) 568, 662
BlsI GCNGC 3 cut(s) 67, 650, 727
Bme1390I CCNGG 2 cut(s) 104, 168
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 4 cut(s) 100, 101, 636, 674
BmiI GGNNCC 4 cut(s) 102, 537, 637, 684
BmrFI CCNGG 2 cut(s) 104, 168
BmsI GCATC 1 cut(s) 811
Bpu10I CCTNAGC 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 167, 568, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 663, 985
Bse21I CCTNAGG 1 cut(s) 995
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 167, 568, 662
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 2 cut(s) 663, 985
BseMII CTCAG 2 cut(s) 590, 986
BseSI GKGCMC 3 cut(s) 104, 760, 1073
BseXI GCAGC 2 cut(s) 52, 660
BshFI GGCC 4 cut(s) 102, 166, 638, 725
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 663, 985
BsmAI GTCTC 1 cut(s) 434
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 4 cut(s) 102, 166, 638, 725
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 5 cut(s) 104, 577, 760, 793, 1073
Bsp143I GATC 6 cut(s) 273, 325, 420, 487, 559, 871
BspACI CCGC 4 cut(s) 75, 687, 726, 1073
BspANI GGCC 4 cut(s) 102, 166, 638, 725
BspCNI CTCAG 2 cut(s) 589, 987
BspLI GGNNCC 4 cut(s) 102, 537, 637, 684
BspMI ACCTGC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 689
BssECI CCNNGG 3 cut(s) 167, 568, 662
BssMI GATC 6 cut(s) 273, 325, 420, 487, 559, 871
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 2 cut(s) 568, 662
Bst2BI CACGAG 1 cut(s) 792
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 4 cut(s) 197, 498, 503, 700
BstC8I GCNNGC 1 cut(s) 1073
BstDEI CTNAG 4 cut(s) 431, 576, 995, 1052
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 65, 415
BstKTI GATC 6 cut(s) 276, 328, 423, 490, 562, 874
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 6 cut(s) 273, 325, 420, 487, 559, 871
BstMWI GCNNNNNNNGC 4 cut(s) 635, 657, 666, 725
BstNI CCWGG 1 cut(s) 168
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 102, 166
BstSLI GKGCMC 3 cut(s) 104, 760, 1073
BstV1I GCAGC 2 cut(s) 52, 660
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu36I CCTNAGG 1 cut(s) 995
BsuI GTATCC 1 cut(s) 903
BsuRI GGCC 4 cut(s) 102, 166, 638, 725
BtgZI GCGATG 2 cut(s) 734, 788
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 442
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 1073
CfoI GCGC 2 cut(s) 65, 415
Cfr13I GGNCC 4 cut(s) 100, 101, 636, 674
Csp6I GTAC 3 cut(s) 259, 499, 976
CviAII CATG 2 cut(s) 183, 585
CviQI GTAC 3 cut(s) 259, 499, 976
DdeI CTNAG 4 cut(s) 431, 576, 995, 1052
DpnI GATC 6 cut(s) 275, 327, 422, 489, 561, 873
DpnII GATC 6 cut(s) 273, 325, 420, 487, 559, 871
EaeI YGGCCR 2 cut(s) 164, 723
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 2 cut(s) 568, 662
Eco24I GRGCYC 3 cut(s) 104, 577, 793
Eco47I GGWCC 1 cut(s) 674
Eco47III AGCGCT 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 575
Eco81I CCTNAGG 1 cut(s) 995
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 568, 662
EcoT38I GRGCYC 3 cut(s) 104, 577, 793
ErhI CCWWGG 2 cut(s) 568, 662
FaeI CATG 2 cut(s) 186, 588
FatI CATG 2 cut(s) 182, 584
FauI CCCGC 2 cut(s) 694, 1080
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 2 cut(s) 524, 1081
Fnu4HI GCNGC 3 cut(s) 66, 649, 726
FokI GGATG 1 cut(s) 39
FriOI GRGCYC 3 cut(s) 104, 577, 793
Fsp4HI GCNGC 3 cut(s) 66, 649, 726
FspBI CTAG 4 cut(s) 225, 557, 569, 663
GlaI GCGC 2 cut(s) 64, 414
GluI GCNGC 3 cut(s) 66, 649, 726
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 4 cut(s) 102, 166, 638, 725
HapII CCGG 1 cut(s) 104
HhaI GCGC 2 cut(s) 65, 415
Hin1II CATG 2 cut(s) 186, 588
Hin6I GCGC 2 cut(s) 63, 413
HinP1I GCGC 2 cut(s) 63, 413
HincII GTYRAC 3 cut(s) 525, 582, 1082
HindII GTYRAC 3 cut(s) 525, 582, 1082
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 2 cut(s) 887, 901
HpaII CCGG 1 cut(s) 104
HphI GGTGA 5 cut(s) 330, 809, 826, 923, 983
Hpy166II GTNNAC 3 cut(s) 525, 582, 1082
Hpy188I TCNGA 3 cut(s) 682, 879, 922
Hpy188III TCNNGA 3 cut(s) 491, 563, 869
Hpy8I GTNNAC 3 cut(s) 525, 582, 1082
Hpy99I CGWCG 3 cut(s) 469, 507, 682
HpyAV CCTTC 4 cut(s) 58, 352, 858, 1050
HpyCH4III ACNGT 4 cut(s) 197, 498, 503, 700
HpyCH4IV ACGT 1 cut(s) 1067
HpyCH4V TGCA 7 cut(s) 186, 217, 714, 764, 1014, 1038, 1064
HpyF10VI GCNNNNNNNGC 4 cut(s) 635, 657, 666, 725
HpyF3I CTNAG 4 cut(s) 431, 576, 995, 1052
HpySE526I ACGT 1 cut(s) 1067
Hsp92II CATG 2 cut(s) 186, 588
HspAI GCGC 2 cut(s) 63, 413
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 6 cut(s) 273, 325, 420, 487, 559, 871
LmnI GCTCC 2 cut(s) 95, 572
Lsp1109I GCAGC 2 cut(s) 52, 660
LweI GCATC 1 cut(s) 811
MaeI CTAG 4 cut(s) 225, 557, 569, 663
MaeII ACGT 1 cut(s) 1067
MaeIII GTNAC 2 cut(s) 134, 268
MalI GATC 6 cut(s) 275, 327, 422, 489, 561, 873
MbiI CCGCTC 1 cut(s) 689
MboI GATC 6 cut(s) 273, 325, 420, 487, 559, 871
MboII GAAGA 3 cut(s) 10, 19, 213
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 5 cut(s) 104, 577, 760, 793, 1073
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 8 cut(s) 154, 230, 334, 402, 732, 821, 923, 1033
MluNI TGGCCA 1 cut(s) 166
MmeI TCCRAC 3 cut(s) 660, 900, 902
MnlI CCTC 9 cut(s) 53, 66, 91, 157, 305, 332, 372, 909, 990
Mox20I TGGCCA 1 cut(s) 166
MscI TGGCCA 1 cut(s) 166
MslI CAYNNNNRTG 2 cut(s) 458, 719
Msp20I TGGCCA 1 cut(s) 166
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 2 cut(s) 104, 168
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 635, 657, 666, 725
NciI CCSGG 1 cut(s) 104
NdeII GATC 6 cut(s) 273, 325, 420, 487, 559, 871
NlaIII CATG 2 cut(s) 186, 588
NlaIV GGNNCC 4 cut(s) 102, 537, 637, 684
PaqCI CACCTGC 1 cut(s) 80
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 2 cut(s) 887, 901
PflFI GACNNNGTC 1 cut(s) 586
PkrI GCNGC 3 cut(s) 67, 650, 727
PsiI TTATAA 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 577
Psp6I CCWGG 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 102, 537, 637, 684
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 4 cut(s) 100, 101, 636, 674
PsuI RGATCY 1 cut(s) 559
PsyI GACNNNGTC 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 126
RsaI GTAC 3 cut(s) 260, 500, 977
RsaNI GTAC 3 cut(s) 259, 499, 976
RseI CAYNNNNRTG 2 cut(s) 458, 719
SacI GAGCTC 1 cut(s) 577
SalI GTCGAC 2 cut(s) 523, 1080
SatI GCNGC 3 cut(s) 66, 649, 726
Sau3AI GATC 6 cut(s) 273, 325, 420, 487, 559, 871
Sau96I GGNCC 4 cut(s) 100, 101, 636, 674
ScrFI CCNGG 2 cut(s) 104, 168
SduI GDGCHC 5 cut(s) 104, 577, 760, 793, 1073
SfaNI GCATC 1 cut(s) 811
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 2 cut(s) 458, 719
Sse9I AATT 8 cut(s) 154, 230, 334, 402, 732, 821, 923, 1033
SsiI CCGC 4 cut(s) 75, 687, 726, 1073
SspMI CTAG 4 cut(s) 225, 557, 569, 663
SstI GAGCTC 1 cut(s) 577
StyD4I CCNGG 2 cut(s) 102, 166
StyI CCWWGG 2 cut(s) 568, 662
TaaI ACNGT 4 cut(s) 197, 498, 503, 700
TaiI ACGT 1 cut(s) 1070
TaqI TCGA 7 cut(s) 83, 249, 324, 524, 855, 885, 1081
TasI AATT 8 cut(s) 154, 230, 334, 402, 732, 821, 923, 1033
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 2 cut(s) 887, 901
TscAI CASTG 1 cut(s) 442
TseI GCWGC 2 cut(s) 65, 648
TspDTI ATGAA 1 cut(s) 223
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 442
Tth111I GACNNNGTC 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 4 cut(s) 230, 334, 402, 923
XmaJI CCTAGG 2 cut(s) 568, 662
XmiI GTMKAC 2 cut(s) 524, 1081
XspI CTAG 4 cut(s) 225, 557, 569, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.