RLG00000004162

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000675
Physical Location & Seq
Forward (+)
56796298 .. 56797575
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004162

Sequence Viewer

Length: 1131 bp
ATGGCTACCTTAGAATTCTTGACCTTGTTCTTCTTCGTCTTCCTTCCGCATATATCTGCTAAGAAATGCCATGAGTTGAGATGCAGGACTTATGACAGCCCACAGGTTAGATTTCCATTCCGGTTAAGAGAGCGCCAACCACCACGTTGTGGATATTCCAGCAACTTTGATCTCTCATGCAACCAACAAAAACAAACCATTCTCACTCTCCCATCATCTTCGGGGGACTTCACCGTCGAGAGCATCAGCTACACCGACCAAAGCATCTGGATCAACGACCAGAACAAGTGCATCCCCAAGCGGTTACTCGACAACGACTTCCGTCTTGCAGGCTCACCCTTCCAATTTGAGCATGGCCTGGAAAACTACACCTACTGCAACTGCTCGCCCCAAGCTCCGCTAATACGGCTTTATACGTATCATCCCATCACTTGCCTCAGCAGTGACAACTACAAGGTCATTGCTTTGCCAACGAGGTCATGGAGGTCGTCGCCTCAGTCTCTTTGTAACATGATTTCGACAGCTATGGTCCCGATCGTAAATTATGAGTGGCTTGACACTGACTTTGGTGTGAGGTTGACATGGGGTGCGCCTGATTGTCGTTCTTGTGAATCGAGTGGTCGAGTTTGTGGGTTTAAGAATGTTTCCAGTTTGCAACTTGACTGCTCTGCGCAAACTAACGGGTTTTCAGGTCTCTCAAATGCCAAAAAGTTCCGGTTACTACTAAGCTTGGGAGTTCCGGGGCTAGTGTGCATCACTATGTTCGCACTTTATATTTACACCTTAATCAGGGACCGTGGTCGCATCAATGAACCCATCACAGAATTATCCAGTGTGACCATTCCACAGATTCATGTCGTTATAACGGGCCTTAATGATCTAACCATTGAATCATATCCGAAGACTCAACTTGGCGAGAAATGGGAATTTCCCAGTCCCGAGGACAGCACTTGCCCAATTTGTTTGTGCGAGTACCAACCCGAGGAAACTATAAGGACAATACCTGAATGCAATCATCATTTTCATGCCAGTTGCATTGATGAATGGCTCAGAAAGAATCCTACGTGCCCAGTTTGTCGAAAAGTTCCGCAGGAGGATAACAAAACAAGTCTCTCCCCAATTGAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

377

Amino Acids

42.85

Weight (kDa)

6.45

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 23 - 93 5.3e-17 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 317 - 360 7.1e-15 Ring finger domain
zf-rbx1 PF12678 317 - 360 1.2e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 318 - 346 4e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 318 - 359 4.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 318 - 359 2.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 863
AasI GACNNNNNNGTC 1 cut(s) 233
Acc16I TGCGCA 1 cut(s) 672
AccB7I CCANNNNNTGG 1 cut(s) 149
AciI CCGC 4 cut(s) 47, 301, 398, 1088
AclWI GGATC 1 cut(s) 278
AcsI RAATTY 2 cut(s) 14, 926
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 1 cut(s) 974
AfiI CCNNNNNNNGG 3 cut(s) 149, 789, 982
AgsI TTSAA 1 cut(s) 890
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 4 cut(s) 249, 395, 524, 729
AluI AGCT 4 cut(s) 249, 395, 524, 729
Alw26I GTCTC 4 cut(s) 504, 698, 1115, 1118
AlwI GGATC 1 cut(s) 278
Ama87I CYCGRG 2 cut(s) 938, 980
AoxI GGCC 2 cut(s) 355, 868
ApoI RAATTY 2 cut(s) 14, 926
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AspLEI GCGC 3 cut(s) 135, 592, 673
AspS9I GGNCC 3 cut(s) 529, 793, 868
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 2 cut(s) 223, 327
AvaI CYCGRG 2 cut(s) 938, 980
AvaII GGWCC 2 cut(s) 529, 793
BaeGI GKGCMC 1 cut(s) 1070
BbsI GAAGAC 2 cut(s) 31, 908
BbvCI CCTCAGC 1 cut(s) 437
BccI CCATC 3 cut(s) 220, 434, 824
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 4 cut(s) 504, 698, 1115, 1118
BfaI CTAG 1 cut(s) 746
BfoI RGCGCY 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 359, 741
Bme18I GGWCC 2 cut(s) 529, 793
BmeT110I CYCGRG 2 cut(s) 938, 980
BmgT120I GGNCC 3 cut(s) 529, 793, 868
BmiI GGNNCC 2 cut(s) 531, 794
BmrFI CCNGG 2 cut(s) 359, 741
BmrI ACTGGG 2 cut(s) 927, 1064
BmsI GCATC 6 cut(s) 71, 252, 273, 300, 762, 813
BmuI ACTGGG 2 cut(s) 927, 1064
BoxI GACNNNNGTC 2 cut(s) 321, 798
BpiI GAAGAC 2 cut(s) 31, 908
Bpu10I CCTNAGC 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 741
BsaAI YACGTR 2 cut(s) 417, 1065
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 4 cut(s) 740, 796, 939, 981
BsaWI WCCGGW 2 cut(s) 120, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 789, 982
Bse1I ACTGG 5 cut(s) 648, 831, 933, 1029, 1070
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 4 cut(s) 740, 796, 939, 981
BseGI GGATG 2 cut(s) 291, 421
BseLI CCNNNNNNNGG 3 cut(s) 149, 789, 982
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 3 cut(s) 451, 509, 1063
BseNI ACTGG 5 cut(s) 648, 831, 933, 1029, 1070
BseSI GKGCMC 1 cut(s) 1070
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 2 cut(s) 357, 870
BsiEI CGRYCG 1 cut(s) 537
BsiHKCI CYCGRG 2 cut(s) 938, 980
BsiSI CCGG 3 cut(s) 121, 715, 740
BslFI GGGAC 4 cut(s) 239, 515, 806, 921
BslI CCNNNNNNNGG 3 cut(s) 149, 789, 982
BsmAI GTCTC 4 cut(s) 504, 698, 1115, 1118
BsmFI GGGAC 4 cut(s) 239, 515, 806, 921
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 2 cut(s) 357, 870
Bso31I GGTCTC 1 cut(s) 698
BsoBI CYCGRG 2 cut(s) 938, 980
Bsp1286I GDGCHC 1 cut(s) 1070
Bsp143I GATC 4 cut(s) 169, 270, 534, 877
BspACI CCGC 4 cut(s) 47, 301, 398, 1088
BspANI GGCC 2 cut(s) 357, 870
BspCNI CTCAG 3 cut(s) 450, 508, 1062
BspLI GGNNCC 2 cut(s) 531, 794
BspPI GGATC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 5 cut(s) 648, 831, 933, 1029, 1070
BssECI CCNNGG 4 cut(s) 740, 796, 939, 981
BssMI GATC 4 cut(s) 169, 270, 534, 877
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 2 cut(s) 235, 797
BstBAI YACGTR 2 cut(s) 417, 1065
BstC8I GCNNGC 2 cut(s) 331, 386
BstDEI CTNAG 6 cut(s) 10, 60, 437, 495, 725, 1049
BstDSI CCRYGG 1 cut(s) 796
BstENI CCTNNNNNAGG 1 cut(s) 787
BstF5I GGATG 2 cut(s) 291, 421
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 3 cut(s) 135, 592, 673
BstKTI GATC 4 cut(s) 172, 273, 537, 880
BstMAI GTCTC 4 cut(s) 504, 698, 1115, 1118
BstMBI GATC 4 cut(s) 169, 270, 534, 877
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 1 cut(s) 406
BstNI CCWGG 1 cut(s) 359
BstPAI GACNNNNGTC 2 cut(s) 321, 798
BstSCI CCNGG 2 cut(s) 357, 739
BstSLI GKGCMC 1 cut(s) 1070
BstSNI TACGTA 1 cut(s) 417
BstV2I GAAGAC 2 cut(s) 31, 908
BsuRI GGCC 2 cut(s) 357, 870
BtgI CCRYGG 1 cut(s) 796
BtsCI GGATG 2 cut(s) 291, 421
BtsI GCAGTG 1 cut(s) 448
BtsIMutI CAGTG 3 cut(s) 448, 558, 838
Cac8I GCNNGC 2 cut(s) 331, 386
CfoI GCGC 3 cut(s) 135, 592, 673
Cfr13I GGNCC 3 cut(s) 529, 793, 868
Csp6I GTAC 1 cut(s) 973
CviAII CATG 9 cut(s) 71, 177, 353, 480, 511, 582, 854, 1025, 1128
CviQI GTAC 1 cut(s) 973
DdeI CTNAG 6 cut(s) 10, 60, 437, 495, 725, 1049
DpnI GATC 4 cut(s) 171, 272, 536, 879
DpnII GATC 4 cut(s) 169, 270, 534, 877
DraIII CACNNNGTG 1 cut(s) 149
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
Eco105I TACGTA 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 529, 793
Eco88I CYCGRG 2 cut(s) 938, 980
EcoNI CCTNNNNNAGG 1 cut(s) 787
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 357
FaeI CATG 9 cut(s) 74, 180, 356, 483, 514, 585, 857, 1028, 1131
FaqI GGGAC 4 cut(s) 239, 515, 806, 921
FatI CATG 9 cut(s) 70, 176, 352, 479, 510, 581, 853, 1024, 1127
FokI GGATG 2 cut(s) 278, 408
FspBI CTAG 1 cut(s) 746
FspI TGCGCA 1 cut(s) 672
GlaI GCGC 3 cut(s) 134, 591, 672
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 2 cut(s) 357, 870
HapII CCGG 3 cut(s) 121, 715, 740
HhaI GCGC 3 cut(s) 135, 592, 673
Hin1II CATG 9 cut(s) 74, 180, 356, 483, 514, 585, 857, 1028, 1131
Hin6I GCGC 3 cut(s) 133, 590, 671
HinP1I GCGC 3 cut(s) 133, 590, 671
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 727
HinfI GANTC 5 cut(s) 611, 850, 890, 904, 1057
HpaII CCGG 3 cut(s) 121, 715, 740
HphI GGTGA 2 cut(s) 223, 327
Hpy166II GTNNAC 1 cut(s) 579
Hpy188I TCNGA 2 cut(s) 900, 1052
Hpy188III TCNNGA 5 cut(s) 19, 238, 268, 532, 938
Hpy8I GTNNAC 1 cut(s) 579
Hpy99I CGWCG 2 cut(s) 239, 493
HpyAV CCTTC 2 cut(s) 53, 349
HpyCH4III ACNGT 2 cut(s) 235, 797
HpyCH4IV ACGT 3 cut(s) 145, 416, 1064
HpyCH4V TGCA 9 cut(s) 84, 180, 291, 329, 378, 655, 753, 1011, 1035
HpyF10VI GCNNNNNNNGC 1 cut(s) 406
HpyF3I CTNAG 6 cut(s) 10, 60, 437, 495, 725, 1049
HpySE526I ACGT 3 cut(s) 145, 416, 1064
Hsp92II CATG 9 cut(s) 74, 180, 356, 483, 514, 585, 857, 1028, 1131
HspAI GCGC 3 cut(s) 133, 590, 671
Kzo9I GATC 4 cut(s) 169, 270, 534, 877
LmnI GCTCC 1 cut(s) 400
LweI GCATC 6 cut(s) 71, 252, 273, 300, 762, 813
MaeI CTAG 1 cut(s) 746
MaeII ACGT 3 cut(s) 145, 416, 1064
MaeIII GTNAC 5 cut(s) 303, 443, 506, 717, 835
MalI GATC 4 cut(s) 171, 272, 536, 879
MboI GATC 4 cut(s) 169, 270, 534, 877
MboII GAAGA 5 cut(s) 22, 25, 31, 210, 913
MfeI CAATTG 1 cut(s) 1119
MhlI GDGCHC 1 cut(s) 1070
MluCI AATT 7 cut(s) 14, 344, 541, 824, 926, 957, 1119
MlyI GAGTC 1 cut(s) 898
MnlI CCTC 8 cut(s) 446, 468, 477, 504, 567, 934, 976, 1087
MseI TTAA 4 cut(s) 125, 636, 785, 873
MslI CAYNNNNRTG 2 cut(s) 758, 1023
MspI CCGG 3 cut(s) 121, 715, 740
MspR9I CCNGG 2 cut(s) 359, 741
MunI CAATTG 1 cut(s) 1119
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 406
NciI CCSGG 1 cut(s) 741
NdeII GATC 4 cut(s) 169, 270, 534, 877
NlaIII CATG 9 cut(s) 74, 180, 356, 483, 514, 585, 857, 1028, 1131
NlaIV GGNNCC 2 cut(s) 531, 794
NmuCI GTSAC 2 cut(s) 443, 835
NsbI TGCGCA 1 cut(s) 672
PctI GAATGC 1 cut(s) 1013
PfeI GAWTC 4 cut(s) 611, 850, 890, 1057
PflMI CCANNNNNTGG 1 cut(s) 149
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 1 cut(s) 898
PpsI GAGTC 1 cut(s) 898
Ppu21I YACGTR 2 cut(s) 417, 1065
PshAI GACNNNNGTC 2 cut(s) 321, 798
PsiI TTATAA 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 531, 794
PspPI GGNCC 3 cut(s) 529, 793, 868
PvuI CGATCG 1 cut(s) 537
RsaI GTAC 1 cut(s) 974
RsaNI GTAC 1 cut(s) 973
RseI CAYNNNNRTG 2 cut(s) 758, 1023
SaqAI TTAA 4 cut(s) 125, 636, 785, 873
Sau3AI GATC 4 cut(s) 169, 270, 534, 877
Sau96I GGNCC 3 cut(s) 529, 793, 868
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 2 cut(s) 359, 741
SduI GDGCHC 1 cut(s) 1070
SfaNI GCATC 6 cut(s) 71, 252, 273, 300, 762, 813
SinI GGWCC 2 cut(s) 529, 793
SmiMI CAYNNNNRTG 2 cut(s) 758, 1023
SnaBI TACGTA 1 cut(s) 417
Sse9I AATT 7 cut(s) 14, 344, 541, 824, 926, 957, 1119
SsiI CCGC 4 cut(s) 47, 301, 398, 1088
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 2 cut(s) 357, 739
TaaI ACNGT 2 cut(s) 235, 797
TaiI ACGT 3 cut(s) 148, 419, 1067
TaqI TCGA 6 cut(s) 237, 309, 518, 614, 622, 1078
TasI AATT 7 cut(s) 14, 344, 541, 824, 926, 957, 1119
TfiI GAWTC 4 cut(s) 611, 850, 890, 1057
Tru1I TTAA 4 cut(s) 125, 636, 785, 873
Tru9I TTAA 4 cut(s) 125, 636, 785, 873
TscAI CASTG 3 cut(s) 448, 565, 838
TseFI GTSAC 2 cut(s) 443, 835
Tsp45I GTSAC 2 cut(s) 443, 835
TspDTI ATGAA 4 cut(s) 825, 842, 1013, 1056
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 3 cut(s) 448, 565, 838
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 2 cut(s) 529, 793
XagI CCTNNNNNAGG 1 cut(s) 787
XapI RAATTY 2 cut(s) 14, 926
XcmI CCANNNNNNNNNTGG 2 cut(s) 350, 477
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.