Rroxscaffold_3G00260920

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000675
Physical Location & Seq
Reverse (-)
55242223 .. 55243511
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260920.1

Sequence Viewer

Length: 1158 bp
ATGGCAACCATAATCACATTGCTGCTATTGTTCTTCTCCTTCTTCTTCTTCTTCCTTCCTCACATAGCAGCTACAACCTGCGGCGCATCAGCATGCGCTGCCAGCGGCCCGTCAGTTCGATTCCCGTTCCGGCTAACTGATCGCCAGCAAACGGGTTGTGGGTATTCCGGTAAGTTTGATGTATCGTGCAACGGACACAAACAAACCATTCTTAACCTCCCAGCTTCCGGAAACTTCACGGTTGAGAGCGTCAGTTACAGGGACCAACTCGTGTGGATCAACGATCGCGACAACTGCATCTTCAAGCGGTTTCTTGACAAGGACTTCAGTCTCATAGGTTCGCCCTTCCGGTACGCGCTTGACCTTAAAGAGTACACCTTCTACAAATGTTCGCCCCAACTTGTGCCACTGTGGTCTCCCATCTCCTGTCTCAGCAGTGACAACTATGAGGTCATTGGCGTTGCGAGCTGGTCAACTTTATCGAGACCACCTTCTCCCTATTGTGATGTGATCACCAGAGCTTTGGTTCCAGCAGAAAGTTATGAGTTAGGGAATTCTGATTTTGGTGTGGGGCTGACTTGGAAGGAGCCTGATTGTCGTTCTTGTGAAGCTAGTGGTAAGGTTTGTGGGTTTCAGAATGGTTCACAGTCGAAAATTCACTGCTCTAGTACTCTATCTTATGCCTCTTCAGGTCTCTCAAAAGCTACGAAGTATGGCATAGCGATTGGCGTATTAATTCCAGGGCTATTATCCATTACCTGTTGTGCACTTTATGTCTCAGCCCCGAGAAGGGATCATGGTCGAATCCAAGAATCTACCACCGAATTATCCGGTACTGTAACTACTCGTCAACCTCAAGTATTCATAGCAGGCCTTGATGGTCAGACAATTGAGTCATATCCCAAGACTCAACTCGGCGAGAGTTTGGAATTGCCTGACCCTAATGACAAAACTTGTCCAATATGTCTGGGTGAGTACCAACCGAAGGAAACTCTGAGGACTATACCAGAATGTAACCATTATTTTCATGCTAATTGCATTGACGAATGGCTTAGAAAAAATCCGACATGCCCGCTTTGTCGCAATCTACCCGGTCTTTGTCGAAATCCACTGGAGGTGTCATCAGTGAGACCACGTGATGATTATGCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

385

Amino Acids

42.6

Weight (kDa)

6.59

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 95 4.9e-14 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 316 - 361 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 318 - 361 1.2e-14 Ring finger domain
zf-C3HC4_2 PF13923 319 - 360 3e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 319 - 347 2.6e-09 RING-like zinc finger
zf-C3HC4 PF00097 319 - 360 1.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 319 - 358 6.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 892
Acc36I ACCTGC 1 cut(s) 86
AccII CGCG 2 cut(s) 288, 356
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 4 cut(s) 81, 105, 307, 1073
AclWI GGATC 2 cut(s) 284, 801
AcsI RAATTY 2 cut(s) 553, 654
AcuI CTGAAG 2 cut(s) 310, 672
AcvI CACGTG 1 cut(s) 1136
AfaI GTAC 5 cut(s) 353, 374, 670, 835, 977
AfiI CCNNNNNNNGG 5 cut(s) 151, 227, 789, 790, 985
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 1 cut(s) 739
AluBI AGCT 6 cut(s) 71, 224, 468, 521, 611, 704
AluI AGCT 6 cut(s) 71, 224, 468, 521, 611, 704
Alw21I GWGCWC 1 cut(s) 769
Alw26I GTCTC 7 cut(s) 335, 420, 434, 478, 698, 781, 1123
Alw44I GTGCAC 1 cut(s) 765
AlwI GGATC 2 cut(s) 284, 801
Ama87I CYCGRG 1 cut(s) 784
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 2 cut(s) 106, 871
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 3 cut(s) 22, 68, 98
ApoI RAATTY 2 cut(s) 553, 654
AseI ATTAAT 1 cut(s) 734
AspLEI GCGC 3 cut(s) 86, 98, 358
AspS9I GGNCC 2 cut(s) 107, 262
AsuC2I CCSGG 1 cut(s) 1092
AsuHPI GGTGA 2 cut(s) 505, 983
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 769
BauI CACGAG 1 cut(s) 269
BbrPI CACGTG 1 cut(s) 1136
Bbv12I GWGCWC 1 cut(s) 769
BbvI GCAGC 3 cut(s) 9, 80, 85
BccI CCATC 2 cut(s) 428, 872
BciT130I CCWGG 1 cut(s) 741
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 1 cut(s) 1092
BcoDI GTCTC 7 cut(s) 335, 420, 434, 478, 698, 781, 1123
BfaI CTAG 2 cut(s) 612, 666
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 5 cut(s) 23, 69, 82, 99, 106
BlsI GCNGC 5 cut(s) 24, 70, 83, 100, 107
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 741, 1092
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 784
BmgT120I GGNCC 2 cut(s) 107, 262
BmiI GGNNCC 3 cut(s) 263, 528, 588
BmrFI CCNGG 2 cut(s) 741, 1092
BmsI GCATC 2 cut(s) 95, 306
BoxI GACNNNNGTC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 1133
BpuEI CTTGAG 1 cut(s) 840
BpuMI CCSGG 1 cut(s) 1092
BsaAI YACGTR 1 cut(s) 1136
BsaI GGTCTC 4 cut(s) 420, 478, 698, 1123
BsaJI CCNNGG 1 cut(s) 740
BsaWI WCCGGW 4 cut(s) 167, 227, 348, 830
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 227, 789, 790, 985
Bse1I ACTGG 1 cut(s) 1116
Bse3DI GCAATG 1 cut(s) 17
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 740
BseLI CCNNNNNNNGG 5 cut(s) 151, 227, 789, 790, 985
BseMI GCAATG 1 cut(s) 17
BseMII CTCAG 3 cut(s) 445, 792, 986
BseNI ACTGG 1 cut(s) 1116
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 3 cut(s) 9, 80, 85
BseYI CCCAGC 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 288, 356
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 108, 873
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 769
BsiHKCI CYCGRG 1 cut(s) 784
BsiSI CCGG 6 cut(s) 130, 168, 228, 349, 831, 1092
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 5 cut(s) 151, 227, 789, 790, 985
BsmAI GTCTC 7 cut(s) 335, 420, 434, 478, 698, 781, 1123
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 2 cut(s) 108, 873
Bso31I GGTCTC 4 cut(s) 420, 478, 698, 1123
BsoBI CYCGRG 1 cut(s) 784
Bsp1286I GDGCHC 1 cut(s) 769
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 5 cut(s) 139, 276, 283, 510, 793
Bsp68I TCGCGA 1 cut(s) 288
BspACI CCGC 4 cut(s) 81, 105, 307, 1073
BspANI GGCC 2 cut(s) 108, 873
BspCNI CTCAG 3 cut(s) 444, 791, 987
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 2 cut(s) 288, 356
BspLI GGNNCC 3 cut(s) 263, 528, 588
BspMI ACCTGC 1 cut(s) 86
BspPI GGATC 2 cut(s) 284, 801
BspTNI GGTCTC 4 cut(s) 420, 478, 698, 1123
BsrDI GCAATG 1 cut(s) 17
BsrI ACTGG 1 cut(s) 1116
BssECI CCNNGG 1 cut(s) 740
BssMI GATC 5 cut(s) 139, 276, 283, 510, 793
BssSI CACGAG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 4 cut(s) 241, 411, 648, 838
Bst6I CTCTTC 1 cut(s) 691
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 1136
BstC8I GCNNGC 6 cut(s) 94, 103, 146, 466, 871, 1073
BstDEI CTNAG 4 cut(s) 431, 778, 995, 1052
BstFNI CGCG 2 cut(s) 288, 356
BstHHI GCGC 3 cut(s) 86, 98, 358
BstKTI GATC 5 cut(s) 142, 279, 286, 513, 796
BstMAI GTCTC 7 cut(s) 335, 420, 434, 478, 698, 781, 1123
BstMBI GATC 5 cut(s) 139, 276, 283, 510, 793
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 4 cut(s) 98, 102, 294, 465
BstNI CCWGG 1 cut(s) 741
BstNSI RCATGY 2 cut(s) 96, 1071
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 739, 1090
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 2 cut(s) 288, 356
BstV1I GCAGC 3 cut(s) 9, 80, 85
BstXI CCANNNNNNTGG 1 cut(s) 523
BsuRI GGCC 2 cut(s) 108, 873
BtsI GCAGTG 2 cut(s) 442, 658
BtsIMutI CAGTG 5 cut(s) 407, 442, 658, 1109, 1131
BtuMI TCGCGA 1 cut(s) 288
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 6 cut(s) 94, 103, 146, 466, 871, 1073
CfoI GCGC 3 cut(s) 86, 98, 358
Cfr13I GGNCC 2 cut(s) 107, 262
CseI GACGC 1 cut(s) 238
Csp6I GTAC 5 cut(s) 352, 373, 669, 834, 976
CspCI CAANNNNNGTGG 2 cut(s) 254, 289
CviAII CATG 4 cut(s) 93, 797, 1028, 1068
CviQI GTAC 5 cut(s) 352, 373, 669, 834, 976
DdeI CTNAG 4 cut(s) 431, 778, 995, 1052
DpnI GATC 5 cut(s) 141, 278, 285, 512, 795
DpnII GATC 5 cut(s) 139, 276, 283, 510, 793
DrdI GACNNNNNNGTC 1 cut(s) 892
DseDI GACNNNNNNGTC 1 cut(s) 892
Eam1104I CTCTTC 1 cut(s) 691
EarI CTCTTC 1 cut(s) 691
Eco147I AGGCCT 1 cut(s) 873
Eco31I GGTCTC 4 cut(s) 420, 478, 698, 1123
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 2 cut(s) 310, 672
Eco72I CACGTG 1 cut(s) 1136
Eco88I CYCGRG 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 739
FaeI CATG 4 cut(s) 96, 800, 1031, 1071
FaqI GGGAC 1 cut(s) 275
FatI CATG 4 cut(s) 92, 796, 1027, 1067
FauI CCCGC 1 cut(s) 1080
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 5 cut(s) 23, 69, 82, 99, 106
Fsp4HI GCNGC 5 cut(s) 23, 69, 82, 99, 106
FspBI CTAG 2 cut(s) 612, 666
GlaI GCGC 3 cut(s) 85, 97, 357
GluI GCNGC 5 cut(s) 23, 69, 82, 99, 106
GsaI CCCAGC 1 cut(s) 224
GsuI CTGGAG 1 cut(s) 1133
HaeIII GGCC 2 cut(s) 108, 873
HapII CCGG 6 cut(s) 130, 168, 228, 349, 831, 1092
HgaI GACGC 1 cut(s) 238
HhaI GCGC 3 cut(s) 86, 98, 358
Hin1II CATG 4 cut(s) 96, 800, 1031, 1071
Hin6I GCGC 3 cut(s) 84, 96, 356
HinP1I GCGC 3 cut(s) 84, 96, 356
HincII GTYRAC 2 cut(s) 474, 851
HindII GTYRAC 2 cut(s) 474, 851
HinfI GANTC 5 cut(s) 120, 804, 812, 893, 907
HpaII CCGG 6 cut(s) 130, 168, 228, 349, 831, 1092
HphI GGTGA 2 cut(s) 505, 983
Hpy166II GTNNAC 5 cut(s) 375, 474, 644, 767, 851
Hpy188I TCNGA 5 cut(s) 559, 636, 885, 996, 1065
Hpy188III TCNNGA 4 cut(s) 228, 287, 314, 483
Hpy8I GTNNAC 5 cut(s) 375, 474, 644, 767, 851
HpyAV CCTTC 8 cut(s) 49, 65, 355, 388, 501, 577, 783, 979
HpyCH4III ACNGT 4 cut(s) 241, 411, 648, 838
HpyCH4IV ACGT 1 cut(s) 1135
HpyCH4V TGCA 4 cut(s) 189, 297, 767, 1038
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 102, 294, 465
HpyF3I CTNAG 4 cut(s) 431, 778, 995, 1052
HpySE526I ACGT 1 cut(s) 1135
Hsp92II CATG 4 cut(s) 96, 800, 1031, 1071
HspAI GCGC 3 cut(s) 84, 96, 356
Kpn2I TCCGGA 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 5 cut(s) 139, 276, 283, 510, 793
LmnI GCTCC 1 cut(s) 586
Lsp1109I GCAGC 3 cut(s) 9, 80, 85
LweI GCATC 2 cut(s) 95, 306
MaeI CTAG 2 cut(s) 612, 666
MaeII ACGT 1 cut(s) 1135
MaeIII GTNAC 4 cut(s) 254, 437, 838, 1013
MalI GATC 5 cut(s) 141, 278, 285, 512, 795
MboI GATC 5 cut(s) 139, 276, 283, 510, 793
MboII GAAGA 7 cut(s) 25, 34, 37, 40, 43, 292, 678
MfeI CAATTG 1 cut(s) 888
MhlI GDGCHC 1 cut(s) 769
MluCI AATT 7 cut(s) 553, 654, 735, 824, 888, 929, 1033
MlyI GAGTC 2 cut(s) 901, 902
MmeI TCCRAC 1 cut(s) 1088
MnlI CCTC 7 cut(s) 69, 227, 442, 694, 864, 990, 1108
MroI TCCGGA 1 cut(s) 227
MseI TTAA 4 cut(s) 213, 366, 734, 1156
MslI CAYNNNNRTG 1 cut(s) 91
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 6 cut(s) 130, 168, 228, 349, 831, 1092
MspR9I CCNGG 2 cut(s) 741, 1092
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 741
MvnI CGCG 2 cut(s) 288, 356
MwoI GCNNNNNNNGC 4 cut(s) 98, 102, 294, 465
NciI CCSGG 1 cut(s) 1092
NdeII GATC 5 cut(s) 139, 276, 283, 510, 793
NlaIII CATG 4 cut(s) 96, 800, 1031, 1071
NlaIV GGNNCC 3 cut(s) 263, 528, 588
NmeAIII GCCGAG 1 cut(s) 894
NmuCI GTSAC 1 cut(s) 437
NruI TCGCGA 1 cut(s) 288
NspI RCATGY 2 cut(s) 96, 1071
PaeI GCATGC 1 cut(s) 96
PceI AGGCCT 1 cut(s) 873
PfeI GAWTC 3 cut(s) 120, 804, 812
PkrI GCNGC 5 cut(s) 24, 70, 83, 100, 107
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 2 cut(s) 901, 901
PmaCI CACGTG 1 cut(s) 1136
PmlI CACGTG 1 cut(s) 1136
PpsI GAGTC 2 cut(s) 901, 901
Ppu21I YACGTR 1 cut(s) 1136
PshAI GACNNNNGTC 1 cut(s) 327
PshBI ATTAAT 1 cut(s) 734
Psp6I CCWGG 1 cut(s) 739
PspCI CACGTG 1 cut(s) 1136
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 3 cut(s) 263, 528, 588
PspPI GGNCC 2 cut(s) 107, 262
PvuI CGATCG 1 cut(s) 286
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 5 cut(s) 353, 374, 670, 835, 977
RsaNI GTAC 5 cut(s) 352, 373, 669, 834, 976
RseI CAYNNNNRTG 1 cut(s) 91
SaqAI TTAA 4 cut(s) 213, 366, 734, 1156
SatI GCNGC 5 cut(s) 23, 69, 82, 99, 106
Sau3AI GATC 5 cut(s) 139, 276, 283, 510, 793
Sau96I GGNCC 2 cut(s) 107, 262
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 2 cut(s) 901, 902
ScrFI CCNGG 2 cut(s) 741, 1092
SduI GDGCHC 1 cut(s) 769
SfaNI GCATC 2 cut(s) 95, 306
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 855
SmoI CTYRAG 1 cut(s) 855
SphI GCATGC 1 cut(s) 96
Sse9I AATT 7 cut(s) 553, 654, 735, 824, 888, 929, 1033
SseBI AGGCCT 1 cut(s) 873
SsiI CCGC 4 cut(s) 81, 105, 307, 1073
SspMI CTAG 2 cut(s) 612, 666
StuI AGGCCT 1 cut(s) 873
StyD4I CCNGG 2 cut(s) 739, 1090
TaaI ACNGT 4 cut(s) 241, 411, 648, 838
TaiI ACGT 1 cut(s) 1138
TaqI TCGA 5 cut(s) 118, 482, 650, 802, 1102
TasI AATT 7 cut(s) 553, 654, 735, 824, 888, 929, 1033
TatI WGTACW 2 cut(s) 372, 668
TauI GCSGC 2 cut(s) 84, 108
TfiI GAWTC 3 cut(s) 120, 804, 812
Tru1I TTAA 4 cut(s) 213, 366, 734, 1156
Tru9I TTAA 4 cut(s) 213, 366, 734, 1156
TscAI CASTG 5 cut(s) 414, 442, 665, 1116, 1131
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 3 cut(s) 22, 68, 98
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 2 cut(s) 853, 1016
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 5 cut(s) 414, 442, 665, 1116, 1131
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 1 cut(s) 262
VspI ATTAAT 1 cut(s) 734
XapI RAATTY 2 cut(s) 553, 654
XceI RCATGY 2 cut(s) 96, 1071
XspI CTAG 2 cut(s) 612, 666
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.