Rh7DG147800

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000675
Physical Location & Seq
Forward (+)
12700257 .. 12702204
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG147800.1

Sequence Viewer

Length: 1215 bp
ATGACTACCTTGGAAATCATCATCACCTTCTCCATCTTGTTTTTCCTTTTTCCGAATCAAACATCAGCAACCTGTCATGAAACAAGATGCAAGGCCAAGGAACCATGGGTACGATTCCCTTTTCAACTACCAAGAGAAGGCCAAGACTTGGATTGTGGCTATGATCGAGGATTTGATCTCTCCTGCAGCAACCACCATAAGCGAACTATCCTCACTCTCCCATCTTCGAGAGACTTCATCGTGAAGGACATCAACTACATCGACCATATCATATGGATCGACGATCCCGGAAGCTGCTTCCCTAAGAGGCTCATGGACCATGACTTCAGTCTTGTGGACTCACCCTTCTCATACTTTTACGGCCTCGAAAGCTACGTGTTCCTCAACTGTTCGTCGACGTCCCGAGAAGAGATACTAGCACCGTCTTCACCAATTGATTGCCTCAGTAGTGAGGACTACAAGGTCATAGCTGTGCAGTCTACTTGGTTCTATGCTGATCTTAATACATCAAGCACTTCATTGAGGCCACTGGCTCAGACATCGTTATCCAGTCTTTGTTCCGTGATCTCCACAGCTATGATTCCACTGTCAGGTTCATTTGACGGGTGGGATCTTAACTTTGGTGTTCGGTTGGCATGGGACATGCCCGATTGTCGTCTATGCGAAGCAAGCGGGAGATTATGTGGGTTTGATGTTGCTACAACTTCGCAAGTTGTTGTGTGCTCTGGACTTTCAAATTCAGTACACCAGAGCAAATCAGGCGTCTCTTTCCGTCTCTCAACTGCAACATACATAGGCGGGGGAATGCTTGTGCTATTGTTCATCTGCGTGTTTGCAATATGTTGTTTTGCTAAACCACCTAGCGATGTACCCACCACAGAATGGTTGGCGGTAACAAATCATCAACGAACTCCTTCGGCATTGGCGCTCAATAGTGTGACTACCGAAGCATATCCCAAGACTCAACTTGGTGATGAGAGTTTGGAACTGCCTAACCCCAATGAAAAAACTTGTGCAATATGTTTGTGCGAGTACCAGCGAAAGGAAACAATAAAGACTATACCACGATGCAACCATTACTTTCATGTTACTTGCATAGATGAGTGGTTTAAAACGAATGTGACGTGCCCACTTTGCCGAAACCCACCGAACAATGAGGGGCTTGGTTTCCCTGGACAACCTTTGCAAGATATGAGTGTTGGTTTCTCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

404

Amino Acids

45.08

Weight (kDa)

5.2

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 95 1.2e-13 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 337 - 380 9.6e-13 Ring finger domain
zf-rbx1 PF12678 337 - 380 2.9e-09 RING-H2 zinc finger domain
zf-RING_11 PF17123 338 - 366 3.9e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 401
AccB7I CCANNNNNTGG 2 cut(s) 148, 882
AccI GTMKAC 2 cut(s) 395, 479
AciI CCGC 3 cut(s) 672, 798, 890
AclWI GGATC 3 cut(s) 278, 284, 618
AcsI RAATTY 1 cut(s) 736
AcuI CTGAAG 1 cut(s) 310
AcyI GRCGYC 2 cut(s) 398, 762
AfaI GTAC 4 cut(s) 111, 744, 870, 1034
AfiI CCNNNNNNNGG 5 cut(s) 137, 148, 590, 882, 1042
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 2 cut(s) 125, 735
AjiI CACGTC 1 cut(s) 1125
AjnI CCWGG 1 cut(s) 1171
AluBI AGCT 4 cut(s) 294, 372, 470, 575
AluI AGCT 4 cut(s) 294, 372, 470, 575
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 3 cut(s) 225, 769, 779
AlwI GGATC 3 cut(s) 278, 284, 618
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 4 cut(s) 93, 139, 361, 524
ApeKI GCWGC 2 cut(s) 186, 294
ApoI RAATTY 1 cut(s) 736
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 1 cut(s) 913
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 4 cut(s) 16, 333, 420, 983
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 1130
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 2 cut(s) 198, 281
BccI CCATC 2 cut(s) 41, 229
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 1173
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 3 cut(s) 225, 769, 779
BfaI CTAG 2 cut(s) 416, 861
BfmI CTRYAG 2 cut(s) 184, 1211
BfoI RGCGCY 1 cut(s) 929
BisI GCNGC 2 cut(s) 187, 295
BlsI GCNGC 2 cut(s) 188, 296
Bme1390I CCNGG 2 cut(s) 288, 1173
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 1125
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 288, 1173
BmsI GCATC 2 cut(s) 77, 1058
BoxI GACNNNNGTC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 376
BsaHI GRCGYC 2 cut(s) 398, 762
BsaJI CCNNGG 4 cut(s) 9, 96, 104, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 5 cut(s) 137, 148, 590, 882, 1042
Bse1I ACTGG 2 cut(s) 534, 549
BseBI CCWGG 1 cut(s) 1173
BseDI CCNNGG 4 cut(s) 9, 96, 104, 1171
BseLI CCNNNNNNNGG 5 cut(s) 137, 148, 590, 882, 1042
BseMII CTCAG 2 cut(s) 457, 548
BseNI ACTGG 2 cut(s) 534, 549
BseSI GKGCMC 1 cut(s) 1130
BseXI GCAGC 2 cut(s) 198, 281
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 4 cut(s) 95, 141, 363, 526
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 2 cut(s) 385, 653
BslI CCNNNNNNNGG 5 cut(s) 137, 148, 590, 882, 1042
BsmAI GTCTC 3 cut(s) 225, 769, 779
BsmBI CGTCTC 2 cut(s) 769, 779
BsmFI GGGAC 2 cut(s) 385, 653
BsmI GAATGC 1 cut(s) 810
BsnI GGCC 4 cut(s) 95, 141, 363, 526
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 725, 1130
Bsp143I GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 3 cut(s) 672, 798, 890
BspANI GGCC 4 cut(s) 95, 141, 363, 526
BspCNI CTCAG 2 cut(s) 456, 547
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 188
BspPI GGATC 3 cut(s) 278, 284, 618
BsrI ACTGG 2 cut(s) 534, 549
BssECI CCNNGG 4 cut(s) 9, 96, 104, 1171
BssMI GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
BssNI GRCGYC 2 cut(s) 398, 762
BssT1I CCWWGG 3 cut(s) 9, 96, 104
Bst2UI CCWGG 1 cut(s) 1173
Bst4CI ACNGT 3 cut(s) 389, 423, 588
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 2 cut(s) 398, 762
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 3 cut(s) 303, 443, 534
BstDSI CCRYGG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 7 cut(s) 166, 178, 279, 286, 499, 567, 613
BstMAI GTCTC 3 cut(s) 225, 769, 779
BstMBI GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
BstMWI GCNNNNNNNGC 4 cut(s) 369, 669, 759, 1134
BstNI CCWGG 1 cut(s) 1173
BstNSI RCATGY 1 cut(s) 646
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 286, 1171
BstSFI CTRYAG 2 cut(s) 184, 1211
BstSLI GKGCMC 1 cut(s) 1130
BstV1I GCAGC 2 cut(s) 198, 281
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 4 cut(s) 95, 141, 363, 526
BtgI CCRYGG 1 cut(s) 104
BtgZI GCGATG 1 cut(s) 879
BtrI CACGTC 1 cut(s) 1125
BtsIMutI CAGTG 2 cut(s) 527, 584
Cac8I GCNNGC 1 cut(s) 670
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 928
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 751
Csp6I GTAC 4 cut(s) 110, 743, 869, 1033
CviAII CATG 7 cut(s) 77, 105, 313, 320, 636, 643, 1085
CviQI GTAC 4 cut(s) 110, 743, 869, 1033
DdeI CTNAG 3 cut(s) 303, 443, 534
DpnI GATC 7 cut(s) 165, 177, 278, 285, 498, 566, 612
DpnII GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
DraI TTTAAA 1 cut(s) 1111
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 3 cut(s) 9, 96, 104
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 1171
EcoT14I CCWWGG 3 cut(s) 9, 96, 104
ErhI CCWWGG 3 cut(s) 9, 96, 104
Esp3I CGTCTC 2 cut(s) 769, 779
FaeI CATG 7 cut(s) 80, 108, 316, 323, 639, 646, 1088
FaqI GGGAC 2 cut(s) 385, 653
FatI CATG 7 cut(s) 76, 104, 312, 319, 635, 642, 1084
FauI CCCGC 2 cut(s) 665, 791
FauNDI CATATG 1 cut(s) 272
FblI GTMKAC 2 cut(s) 395, 479
Fnu4HI GCNGC 2 cut(s) 187, 295
Fsp4HI GCNGC 2 cut(s) 187, 295
FspBI CTAG 2 cut(s) 416, 861
GlaI GCGC 1 cut(s) 927
GluI GCNGC 2 cut(s) 187, 295
HaeII RGCGCY 1 cut(s) 929
HaeIII GGCC 4 cut(s) 95, 141, 363, 526
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 751
HhaI GCGC 1 cut(s) 928
Hin1I GRCGYC 2 cut(s) 398, 762
Hin1II CATG 7 cut(s) 80, 108, 316, 323, 639, 646, 1088
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 5 cut(s) 55, 114, 338, 580, 961
HpaII CCGG 1 cut(s) 288
HphI GGTGA 4 cut(s) 16, 333, 420, 983
Hpy166II GTNNAC 4 cut(s) 337, 396, 480, 745
Hpy188I TCNGA 2 cut(s) 54, 537
Hpy188III TCNNGA 5 cut(s) 77, 228, 241, 402, 726
Hpy8I GTNNAC 4 cut(s) 337, 396, 480, 745
Hpy99I CGWCG 3 cut(s) 284, 397, 400
HpyAV CCTTC 5 cut(s) 37, 131, 238, 355, 924
HpyCH4III ACNGT 3 cut(s) 389, 423, 588
HpyCH4IV ACGT 3 cut(s) 375, 398, 1124
HpyCH4V TGCA 9 cut(s) 90, 186, 475, 785, 836, 1016, 1071, 1095, 1186
HpyF10VI GCNNNNNNNGC 4 cut(s) 369, 669, 759, 1134
HpyF3I CTNAG 3 cut(s) 303, 443, 534
HpySE526I ACGT 3 cut(s) 375, 398, 1124
Hsp92I GRCGYC 2 cut(s) 398, 762
Hsp92II CATG 7 cut(s) 80, 108, 316, 323, 639, 646, 1088
HspAI GCGC 1 cut(s) 926
Kzo9I GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
Lsp1109I GCAGC 2 cut(s) 198, 281
LweI GCATC 2 cut(s) 77, 1058
MaeI CTAG 2 cut(s) 416, 861
MaeII ACGT 3 cut(s) 375, 398, 1124
MaeIII GTNAC 4 cut(s) 892, 937, 1087, 1120
MalI GATC 7 cut(s) 165, 177, 278, 285, 498, 566, 612
MboI GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
MboII GAAGA 3 cut(s) 216, 417, 419
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 2 cut(s) 725, 1130
MluCI AATT 2 cut(s) 432, 736
MlyI GAGTC 2 cut(s) 332, 955
MnlI CCTC 9 cut(s) 161, 221, 300, 374, 392, 445, 452, 516, 1150
MroXI GAANNNNTTC 1 cut(s) 913
MseI TTAA 3 cut(s) 501, 615, 1110
MslI CAYNNNNRTG 3 cut(s) 470, 575, 827
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 288, 1173
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 1 cut(s) 1173
MwoI GCNNNNNNNGC 4 cut(s) 369, 669, 759, 1134
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 104
NdeI CATATG 1 cut(s) 272
NdeII GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
NlaIII CATG 7 cut(s) 80, 108, 316, 323, 639, 646, 1088
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 2 cut(s) 937, 1120
NspI RCATGY 1 cut(s) 646
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 1121
PctI GAATGC 1 cut(s) 810
PdmI GAANNNNTTC 1 cut(s) 913
PfeI GAWTC 3 cut(s) 55, 114, 580
PflMI CCANNNNNTGG 2 cut(s) 148, 882
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 2 cut(s) 188, 296
PleI GAGTC 2 cut(s) 332, 955
PpsI GAGTC 2 cut(s) 332, 955
Ppu21I YACGTR 1 cut(s) 376
PshAI GACNNNNGTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 1171
PspGI CCWGG 1 cut(s) 1171
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 4 cut(s) 111, 744, 870, 1034
RsaNI GTAC 4 cut(s) 110, 743, 869, 1033
RseI CAYNNNNRTG 3 cut(s) 470, 575, 827
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 3 cut(s) 501, 615, 1110
SatI GCNGC 2 cut(s) 187, 295
Sau3AI GATC 7 cut(s) 163, 175, 276, 283, 496, 564, 610
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 2 cut(s) 332, 955
ScrFI CCNGG 2 cut(s) 288, 1173
SduI GDGCHC 2 cut(s) 725, 1130
SfaNI GCATC 2 cut(s) 77, 1058
SfcI CTRYAG 2 cut(s) 184, 1211
SgrDI CGTCGACG 1 cut(s) 394
SinI GGWCC 1 cut(s) 316
SmiMI CAYNNNNRTG 3 cut(s) 470, 575, 827
Sse9I AATT 2 cut(s) 432, 736
SsiI CCGC 3 cut(s) 672, 798, 890
SspMI CTAG 2 cut(s) 416, 861
StyD4I CCNGG 2 cut(s) 286, 1171
StyI CCWWGG 3 cut(s) 9, 96, 104
TaaI ACNGT 3 cut(s) 389, 423, 588
TaiI ACGT 3 cut(s) 378, 401, 1127
TaqI TCGA 6 cut(s) 166, 227, 261, 279, 366, 395
TasI AATT 2 cut(s) 432, 736
TatI WGTACW 1 cut(s) 742
TfiI GAWTC 3 cut(s) 55, 114, 580
Tru1I TTAA 3 cut(s) 501, 615, 1110
Tru9I TTAA 3 cut(s) 501, 615, 1110
TscAI CASTG 2 cut(s) 534, 591
TseFI GTSAC 2 cut(s) 937, 1120
TseI GCWGC 2 cut(s) 186, 294
Tsp45I GTSAC 2 cut(s) 937, 1120
TspDTI ATGAA 7 cut(s) 93, 226, 507, 585, 811, 1017, 1073
TspGWI ACGGA 2 cut(s) 550, 761
TspRI CASTG 2 cut(s) 534, 591
Van91I CCANNNNNTGG 2 cut(s) 148, 882
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 736
XceI RCATGY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 2 cut(s) 395, 479
XmnI GAANNNNTTC 1 cut(s) 913
XspI CTAG 2 cut(s) 416, 861
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.