FvH4_5g04552

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000675
Physical Location & Seq
Reverse (-)
2685492 .. 2686492
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04552.t1

Sequence Viewer

Length: 780 bp
ATGGCTTGCTTGGAAATCTTCACCTCCTTATTCTTCTTCTCCTTCCTTCCACACCTAGCAGCCACCACATGCCGCGGTAAATGCAGTTCCGACGGCCCAGCTGTTCGATTTCCATTCTGGATAGTAGATTACCACTCCTCGCATTGCGGGCGGCTAGGGTTTAGTCTCTCATGCAGCAACCAAAAGCAAACCATTCTTACTCTCCCAAGTTCCAGGGATTTCATTTTCGACAGGATCAAGTACTTGGACAGAACCCTAACGATCAACGACACCAAAAACTGCCTCATTAAGCGATTTCTTGACGATGAAATGGATCTCATGCCTTCACCCTTCTCCTACCTTTATGACTTTGCAAACTACACATTCTTCAATTGCTCGCCCAATGCAAAAAAGCTAGATTGGTATCCAACCCTGTCTTGCCTCAGCAGTGACAACTACAAGGTCATTGCCCTGCCAACGAATCCTTTACTTCGTGCTGACCCTGTTACTACTCCATCTGCGACGTTGTCATCATCGCAGGCTCCATCACCTTTTGATCCTAACTGTTCTGTGATTTCAACAGCTAGGGTTCCAATAGAACCAAAGAATTATGATCCAGAACCACCAAATCCATCCGAGTGGGAGAACATTAACTTGGGTGTTCAGTTAAAGTGGGACGAGCCTGATTGTCGTCATTGTGATGAGCACTTCAAGAGCTGCAGAGATTGCCACAGTGATAGGGGTGGTAGTGTCAGTGGGACTGTGGTTGATCTGGTTTGCATTGAGGCTGATAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

260

Amino Acids

29.24

Weight (kDa)

5.5

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 24 - 90 2.4e-13 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 75
AciI CCGC 4 cut(s) 73, 75, 147, 151
AclWI GGATC 4 cut(s) 242, 321, 530, 587
AfaI GTAC 1 cut(s) 242
AgsI TTSAA 3 cut(s) 370, 558, 691
AjnI CCWGG 1 cut(s) 212
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 4 cut(s) 101, 394, 563, 696
AluI AGCT 4 cut(s) 101, 394, 563, 696
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 4 cut(s) 242, 321, 530, 587
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 3 cut(s) 59, 174, 696
AspS9I GGNCC 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 13, 318, 519
Bbv12I GWGCWC 1 cut(s) 687
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 3 cut(s) 71, 186, 683
BccI CCATC 3 cut(s) 502, 532, 619
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 1 cut(s) 214
BciVI GTATCC 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 4 cut(s) 56, 155, 395, 564
BfmI CTRYAG 1 cut(s) 697
BfuI GTATCC 1 cut(s) 414
BisI GCNGC 5 cut(s) 60, 73, 152, 175, 697
BlsI GCNGC 5 cut(s) 61, 74, 153, 176, 698
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 522, 570
BmrFI CCNGG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 402
BsaJI CCNNGG 2 cut(s) 73, 213
Bse3DI GCAATG 2 cut(s) 142, 444
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 214
BseDI CCNNGG 2 cut(s) 73, 213
BseGI GGATG 1 cut(s) 611
BseJI GATNNNNATC 1 cut(s) 402
BseMI GCAATG 2 cut(s) 142, 444
BseMII CTCAG 1 cut(s) 436
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 3 cut(s) 71, 186, 683
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 687
BslFI GGGAC 2 cut(s) 668, 751
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 668, 751
BsnI GGCC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 6 cut(s) 234, 261, 313, 535, 592, 748
BspACI CCGC 4 cut(s) 73, 75, 147, 151
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 435
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 522, 570
BspMAI CTGCAG 1 cut(s) 701
BspPI GGATC 4 cut(s) 242, 321, 530, 587
BsrDI GCAATG 2 cut(s) 142, 444
BssECI CCNNGG 2 cut(s) 73, 213
BssMI GATC 6 cut(s) 234, 261, 313, 535, 592, 748
Bst2UI CCWGG 1 cut(s) 214
Bst4CI ACNGT 3 cut(s) 545, 713, 742
BstAPI GCANNNNNTGC 1 cut(s) 705
BstC8I GCNNGC 4 cut(s) 7, 149, 377, 519
BstDEI CTNAG 1 cut(s) 422
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 611
BstFNI CGCG 1 cut(s) 75
BstKTI GATC 6 cut(s) 237, 264, 316, 538, 595, 751
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 6 cut(s) 234, 261, 313, 535, 592, 748
BstMWI GCNNNNNNNGC 3 cut(s) 81, 148, 705
BstNI CCWGG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 212
BstSFI CTRYAG 1 cut(s) 697
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 71, 186, 683
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 618
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 73
BtgZI GCGATG 1 cut(s) 498
BtsCI GGATG 1 cut(s) 611
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 433, 718, 739
Cac8I GCNNGC 4 cut(s) 7, 149, 377, 519
Cfr13I GGNCC 1 cut(s) 95
Cfr42I CCGCGG 1 cut(s) 76
Csp6I GTAC 1 cut(s) 241
CviAII CATG 3 cut(s) 69, 171, 319
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 422
DpnI GATC 6 cut(s) 236, 263, 315, 537, 594, 750
DpnII GATC 6 cut(s) 234, 261, 313, 535, 592, 748
EcoRII CCWGG 1 cut(s) 212
FaeI CATG 3 cut(s) 72, 174, 322
FaiI YATR 5 cut(s) 70, 172, 320, 345, 591
FaqI GGGAC 2 cut(s) 668, 751
FatI CATG 3 cut(s) 68, 170, 318
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 5 cut(s) 60, 73, 152, 175, 697
FokI GGATG 1 cut(s) 598
Fsp4HI GCNGC 5 cut(s) 60, 73, 152, 175, 697
FspBI CTAG 4 cut(s) 56, 155, 395, 564
GluI GCNGC 5 cut(s) 60, 73, 152, 175, 697
GsaI CCCAGC 1 cut(s) 101
HaeIII GGCC 1 cut(s) 96
Hin1II CATG 3 cut(s) 72, 174, 322
HinfI GANTC 1 cut(s) 460
HphI GGTGA 3 cut(s) 13, 318, 519
Hpy188I TCNGA 2 cut(s) 91, 616
Hpy188III TCNNGA 4 cut(s) 118, 299, 596, 691
Hpy99I CGWCG 2 cut(s) 95, 505
HpyAV CCTTC 4 cut(s) 52, 56, 333, 340
HpyCH4III ACNGT 3 cut(s) 545, 713, 742
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 6 cut(s) 84, 174, 353, 386, 699, 759
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 148, 705
HpyF3I CTNAG 1 cut(s) 422
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 3 cut(s) 72, 174, 322
KspI CCGCGG 1 cut(s) 76
Kzo9I GATC 6 cut(s) 234, 261, 313, 535, 592, 748
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 3 cut(s) 71, 186, 683
MaeI CTAG 4 cut(s) 56, 155, 395, 564
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 2 cut(s) 428, 484
MalI GATC 6 cut(s) 236, 263, 315, 537, 594, 750
MboI GATC 6 cut(s) 234, 261, 313, 535, 592, 748
MboII GAAGA 4 cut(s) 10, 25, 28, 358
MfeI CAATTG 1 cut(s) 370
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 2 cut(s) 370, 586
MmeI TCCRAC 2 cut(s) 114, 431
MnlI CCTC 5 cut(s) 34, 148, 293, 431, 757
MseI TTAA 3 cut(s) 288, 630, 647
MslI CAYNNNNRTG 2 cut(s) 616, 678
MspA1I CMGCKG 2 cut(s) 75, 101
MspR9I CCNGG 1 cut(s) 214
MunI CAATTG 1 cut(s) 370
MvaI CCWGG 1 cut(s) 214
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 3 cut(s) 81, 148, 705
NdeII GATC 6 cut(s) 234, 261, 313, 535, 592, 748
NlaIII CATG 3 cut(s) 72, 174, 322
NlaIV GGNNCC 2 cut(s) 522, 570
NmuCI GTSAC 1 cut(s) 428
NspI RCATGY 1 cut(s) 72
PfeI GAWTC 1 cut(s) 460
PflFI GACNNNGTC 1 cut(s) 505
PkrI GCNGC 5 cut(s) 61, 74, 153, 176, 698
Psp6I CCWGG 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 2 cut(s) 522, 570
PspPI GGNCC 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 70, 102
PstI CTGCAG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 2 cut(s) 616, 678
SacII CCGCGG 1 cut(s) 76
SaqAI TTAA 3 cut(s) 288, 630, 647
SatI GCNGC 5 cut(s) 60, 73, 152, 175, 697
Sau3AI GATC 6 cut(s) 234, 261, 313, 535, 592, 748
Sau96I GGNCC 1 cut(s) 95
ScaI AGTACT 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 1 cut(s) 687
SfcI CTRYAG 1 cut(s) 697
Sfr303I CCGCGG 1 cut(s) 76
SgrBI CCGCGG 1 cut(s) 76
SmiMI CAYNNNNRTG 2 cut(s) 616, 678
Sse9I AATT 2 cut(s) 370, 586
SsiI CCGC 4 cut(s) 73, 75, 147, 151
SspMI CTAG 4 cut(s) 56, 155, 395, 564
StyD4I CCNGG 1 cut(s) 212
TaaI ACNGT 3 cut(s) 545, 713, 742
TaiI ACGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 106, 228
TasI AATT 2 cut(s) 370, 586
TatI WGTACW 1 cut(s) 240
TauI GCSGC 2 cut(s) 75, 154
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 3 cut(s) 288, 630, 647
Tru9I TTAA 3 cut(s) 288, 630, 647
TscAI CASTG 3 cut(s) 433, 718, 739
TseFI GTSAC 1 cut(s) 428
TseI GCWGC 3 cut(s) 59, 174, 696
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 2 cut(s) 211, 321
TspRI CASTG 3 cut(s) 433, 718, 739
Tth111I GACNNNGTC 1 cut(s) 505
XceI RCATGY 1 cut(s) 72
XspI CTAG 4 cut(s) 56, 155, 395, 564
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.