Rh7AG146600

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000675
Physical Location & Seq
Forward (+)
11889413 .. 11891344
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG146600.1

Sequence Viewer

Length: 1209 bp
ATGACTACCTTGGAAATCATCATCACCTTCTCCATCTTGTTTTTCCTTTTTCCGAATCAAACATCAGCAACCTGTCATGAAACAAGATGCAAGGCCAAGGAACCATGGGTACGATTCCCTTTTCAACTACTAAGAGAAGGCCAAGACTTGGATTGTGGCTATGATCGAGGATTTGATCTCTCCTGCAGCAACCACCATAAGCGAACTATCCTCACTCTCCCATCTTCGAGAGACTTCATCGTGAAGGACATCAACTACATCGACCATATCATATGGATCGACGATCCCGGAAGCTGCTTCCCTAAGAGGCTCATGGACCATGACTTCAGTCTTGTGGACTCACCCTTCTCATACTTTTACGGCCTCGAAAGCTACGTGTTCATCAACTGTTCGTCGACGTCCCGAGAAGAGATACTAGCACCGTCTTCACCAATTGATTGCCTCAGTAGTGAGGACTACAAGGTCATAGCTGTGCAGTCTACTTGGTTCTATGCTGATCTTAATACCACTTCATTGAGGCCACTGGCTCAGACATCGTTATCCCGTCTTTGTTCCGTGATCTCCACAGCTATGATTCCACTGTCAGGTTCATTTGACGGGTGGGATCTTAACTTTGGTGTTCGGTTGGCATGGGACATGCCCGATTGTCGTCTATGCGAAGCAAGCGGGAGATTATGTGGGTTTGATGTTGCTACAACTTCGCAAGTTCCTGTGTGCTCTGGACTTTCAAATTCAGTACACCAGAGAAAATCAGGCGTCTCTTTCGGTCTCTCAACTGCAACATACATAGGCGGGGGAATGCTTGTGCTATTGTTCATCTGCGTGTTTGCAATATGTTGTTTTGCTAAACCACCTAGCGATGTACCCACCACAGAATGGTTGGCGGTAACAAATCATCAACGAACTCCTTCGGCATTGGCGCTCAATAGTGTGACTACCGAAGCATATCCCAAGACTCAACTTGGTGATGAGAGTTTGGAACTGCCTAACCCCAATGAAAAAACTTGTGCAATATGTTTGTGCGAGTACCAGCGAAAGGAAACAATAAAGACTATACCACGATGCAACCATTACTTTCATGTTACTTGCATAGATGAGTGGTTTAAAACGAATGTGACGTGCCCACTTTGCCGAAACCCATCGACCAATGAGGGGCTTGGTTTCCCTGGACAACCTTTGCAAGATGTGAGTGTTGGTTTCTCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

402

Amino Acids

44.9

Weight (kDa)

5.29

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 95 1.3e-13 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 335 - 378 9.5e-13 Ring finger domain
zf-rbx1 PF12678 335 - 378 2.9e-09 RING-H2 zinc finger domain
zf-RING_11 PF17123 336 - 364 3.9e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 401
AccB7I CCANNNNNTGG 2 cut(s) 148, 876
AccI GTMKAC 2 cut(s) 395, 479
AciI CCGC 3 cut(s) 666, 792, 884
AclWI GGATC 3 cut(s) 278, 284, 612
AcsI RAATTY 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 310
AcyI GRCGYC 2 cut(s) 398, 756
AfaI GTAC 4 cut(s) 111, 738, 864, 1028
AfiI CCNNNNNNNGG 5 cut(s) 148, 584, 876, 1036, 1152
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 2 cut(s) 125, 729
AjiI CACGTC 1 cut(s) 1119
AjnI CCWGG 1 cut(s) 1165
AluBI AGCT 4 cut(s) 294, 372, 470, 569
AluI AGCT 4 cut(s) 294, 372, 470, 569
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 3 cut(s) 225, 763, 773
AlwI GGATC 3 cut(s) 278, 284, 612
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 4 cut(s) 93, 139, 361, 518
ApeKI GCWGC 2 cut(s) 186, 294
ApoI RAATTY 1 cut(s) 730
Asp700I GAANNNNTTC 1 cut(s) 907
AspLEI GCGC 1 cut(s) 922
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 4 cut(s) 16, 333, 420, 977
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 316
BaeGI GKGCMC 1 cut(s) 1124
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 719
BbvI GCAGC 2 cut(s) 198, 281
BccI CCATC 3 cut(s) 41, 229, 1147
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 1167
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 3 cut(s) 225, 763, 773
BfaI CTAG 2 cut(s) 416, 855
BfmI CTRYAG 2 cut(s) 184, 1205
BfoI RGCGCY 1 cut(s) 923
BisI GCNGC 2 cut(s) 187, 295
BlsI GCNGC 2 cut(s) 188, 296
Bme1390I CCNGG 2 cut(s) 288, 1167
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 1119
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 288, 1167
BmsI GCATC 2 cut(s) 77, 1052
BoxI GACNNNNGTC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 376
BsaHI GRCGYC 2 cut(s) 398, 756
BsaI GGTCTC 1 cut(s) 773
BsaJI CCNNGG 4 cut(s) 9, 96, 104, 1165
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 584, 876, 1036, 1152
Bse1I ACTGG 1 cut(s) 528
BseBI CCWGG 1 cut(s) 1167
BseDI CCNNGG 4 cut(s) 9, 96, 104, 1165
BseLI CCNNNNNNNGG 5 cut(s) 148, 584, 876, 1036, 1152
BseMII CTCAG 2 cut(s) 457, 542
BseNI ACTGG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 1124
BseXI GCAGC 2 cut(s) 198, 281
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 4 cut(s) 95, 141, 363, 520
BsiHKAI GWGCWC 1 cut(s) 719
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 2 cut(s) 385, 647
BslI CCNNNNNNNGG 5 cut(s) 148, 584, 876, 1036, 1152
BsmAI GTCTC 3 cut(s) 225, 763, 773
BsmBI CGTCTC 1 cut(s) 763
BsmFI GGGAC 2 cut(s) 385, 647
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 4 cut(s) 95, 141, 363, 520
Bso31I GGTCTC 1 cut(s) 773
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 719, 1124
Bsp143I GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 3 cut(s) 666, 792, 884
BspANI GGCC 4 cut(s) 95, 141, 363, 520
BspCNI CTCAG 2 cut(s) 456, 541
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 188
BspPI GGATC 3 cut(s) 278, 284, 612
BspTNI GGTCTC 1 cut(s) 773
BsrI ACTGG 1 cut(s) 528
BssECI CCNNGG 4 cut(s) 9, 96, 104, 1165
BssMI GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
BssNI GRCGYC 2 cut(s) 398, 756
BssT1I CCWWGG 3 cut(s) 9, 96, 104
Bst2UI CCWGG 1 cut(s) 1167
Bst4CI ACNGT 3 cut(s) 389, 423, 582
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 2 cut(s) 398, 756
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 4 cut(s) 131, 303, 443, 528
BstDSI CCRYGG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 923
BstHHI GCGC 1 cut(s) 922
BstKTI GATC 7 cut(s) 166, 178, 279, 286, 499, 561, 607
BstMAI GTCTC 3 cut(s) 225, 763, 773
BstMBI GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
BstMWI GCNNNNNNNGC 3 cut(s) 369, 663, 1128
BstNI CCWGG 1 cut(s) 1167
BstNSI RCATGY 1 cut(s) 640
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 286, 1165
BstSFI CTRYAG 2 cut(s) 184, 1205
BstSLI GKGCMC 1 cut(s) 1124
BstV1I GCAGC 2 cut(s) 198, 281
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 4 cut(s) 95, 141, 363, 520
BtgI CCRYGG 1 cut(s) 104
BtgZI GCGATG 1 cut(s) 873
BtrI CACGTC 1 cut(s) 1119
BtsIMutI CAGTG 2 cut(s) 521, 578
Cac8I GCNNGC 1 cut(s) 664
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 922
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 745
Csp6I GTAC 4 cut(s) 110, 737, 863, 1027
CviAII CATG 7 cut(s) 77, 105, 313, 320, 630, 637, 1079
CviQI GTAC 4 cut(s) 110, 737, 863, 1027
DdeI CTNAG 4 cut(s) 131, 303, 443, 528
DpnI GATC 7 cut(s) 165, 177, 278, 285, 498, 560, 606
DpnII GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
DraI TTTAAA 1 cut(s) 1105
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 3 cut(s) 9, 96, 104
Eco31I GGTCTC 1 cut(s) 773
Eco47I GGWCC 1 cut(s) 316
Eco57I CTGAAG 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 1165
EcoT14I CCWWGG 3 cut(s) 9, 96, 104
ErhI CCWWGG 3 cut(s) 9, 96, 104
Esp3I CGTCTC 1 cut(s) 763
FaeI CATG 7 cut(s) 80, 108, 316, 323, 633, 640, 1082
FaqI GGGAC 2 cut(s) 385, 647
FatI CATG 7 cut(s) 76, 104, 312, 319, 629, 636, 1078
FauI CCCGC 2 cut(s) 659, 785
FauNDI CATATG 1 cut(s) 272
FblI GTMKAC 2 cut(s) 395, 479
Fnu4HI GCNGC 2 cut(s) 187, 295
Fsp4HI GCNGC 2 cut(s) 187, 295
FspBI CTAG 2 cut(s) 416, 855
GlaI GCGC 1 cut(s) 921
GluI GCNGC 2 cut(s) 187, 295
HaeII RGCGCY 1 cut(s) 923
HaeIII GGCC 4 cut(s) 95, 141, 363, 520
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 745
HhaI GCGC 1 cut(s) 922
Hin1I GRCGYC 2 cut(s) 398, 756
Hin1II CATG 7 cut(s) 80, 108, 316, 323, 633, 640, 1082
Hin6I GCGC 1 cut(s) 920
HinP1I GCGC 1 cut(s) 920
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 5 cut(s) 55, 114, 338, 574, 955
HpaII CCGG 1 cut(s) 288
HphI GGTGA 4 cut(s) 16, 333, 420, 977
Hpy166II GTNNAC 4 cut(s) 337, 396, 480, 739
Hpy188I TCNGA 2 cut(s) 54, 531
Hpy188III TCNNGA 5 cut(s) 77, 228, 241, 402, 720
Hpy8I GTNNAC 4 cut(s) 337, 396, 480, 739
Hpy99I CGWCG 3 cut(s) 284, 397, 400
HpyAV CCTTC 5 cut(s) 37, 131, 238, 355, 918
HpyCH4III ACNGT 3 cut(s) 389, 423, 582
HpyCH4IV ACGT 3 cut(s) 375, 398, 1118
HpyCH4V TGCA 9 cut(s) 90, 186, 475, 779, 830, 1010, 1065, 1089, 1180
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 663, 1128
HpyF3I CTNAG 4 cut(s) 131, 303, 443, 528
HpySE526I ACGT 3 cut(s) 375, 398, 1118
Hsp92I GRCGYC 2 cut(s) 398, 756
Hsp92II CATG 7 cut(s) 80, 108, 316, 323, 633, 640, 1082
HspAI GCGC 1 cut(s) 920
Kzo9I GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
Lsp1109I GCAGC 2 cut(s) 198, 281
LweI GCATC 2 cut(s) 77, 1052
MaeI CTAG 2 cut(s) 416, 855
MaeII ACGT 3 cut(s) 375, 398, 1118
MaeIII GTNAC 4 cut(s) 886, 931, 1081, 1114
MalI GATC 7 cut(s) 165, 177, 278, 285, 498, 560, 606
MboI GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
MboII GAAGA 3 cut(s) 216, 417, 419
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 719, 1124
MluCI AATT 2 cut(s) 432, 730
MlyI GAGTC 2 cut(s) 332, 949
MnlI CCTC 8 cut(s) 161, 221, 300, 374, 445, 452, 510, 1144
MroXI GAANNNNTTC 1 cut(s) 907
MseI TTAA 3 cut(s) 501, 609, 1104
MslI CAYNNNNRTG 3 cut(s) 470, 569, 821
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 288, 1167
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 1 cut(s) 1167
MwoI GCNNNNNNNGC 3 cut(s) 369, 663, 1128
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 104
NdeI CATATG 1 cut(s) 272
NdeII GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
NlaIII CATG 7 cut(s) 80, 108, 316, 323, 633, 640, 1082
NlaIV GGNNCC 1 cut(s) 102
NmuCI GTSAC 2 cut(s) 931, 1114
NspI RCATGY 1 cut(s) 640
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 2 cut(s) 372, 1115
PctI GAATGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 3 cut(s) 55, 114, 574
PflMI CCANNNNNTGG 2 cut(s) 148, 876
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 2 cut(s) 188, 296
PleI GAGTC 2 cut(s) 332, 949
PpsI GAGTC 2 cut(s) 332, 949
Ppu21I YACGTR 1 cut(s) 376
PshAI GACNNNNGTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 1165
PspGI CCWGG 1 cut(s) 1165
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 316
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 4 cut(s) 111, 738, 864, 1028
RsaNI GTAC 4 cut(s) 110, 737, 863, 1027
RseI CAYNNNNRTG 3 cut(s) 470, 569, 821
SalI GTCGAC 1 cut(s) 394
SaqAI TTAA 3 cut(s) 501, 609, 1104
SatI GCNGC 2 cut(s) 187, 295
Sau3AI GATC 7 cut(s) 163, 175, 276, 283, 496, 558, 604
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 2 cut(s) 332, 949
ScrFI CCNGG 2 cut(s) 288, 1167
SduI GDGCHC 2 cut(s) 719, 1124
SfaNI GCATC 2 cut(s) 77, 1052
SfcI CTRYAG 2 cut(s) 184, 1205
SgrDI CGTCGACG 1 cut(s) 394
SinI GGWCC 1 cut(s) 316
SmiMI CAYNNNNRTG 3 cut(s) 470, 569, 821
Sse9I AATT 2 cut(s) 432, 730
SsiI CCGC 3 cut(s) 666, 792, 884
SspMI CTAG 2 cut(s) 416, 855
StyD4I CCNGG 2 cut(s) 286, 1165
StyI CCWWGG 3 cut(s) 9, 96, 104
TaaI ACNGT 3 cut(s) 389, 423, 582
TaiI ACGT 3 cut(s) 378, 401, 1121
TaqI TCGA 7 cut(s) 166, 227, 261, 279, 366, 395, 1142
TaqII GACCGA 1 cut(s) 755
TasI AATT 2 cut(s) 432, 730
TatI WGTACW 1 cut(s) 736
TfiI GAWTC 3 cut(s) 55, 114, 574
Tru1I TTAA 3 cut(s) 501, 609, 1104
Tru9I TTAA 3 cut(s) 501, 609, 1104
TscAI CASTG 2 cut(s) 528, 585
TseFI GTSAC 2 cut(s) 931, 1114
TseI GCWGC 2 cut(s) 186, 294
Tsp45I GTSAC 2 cut(s) 931, 1114
TspDTI ATGAA 8 cut(s) 93, 226, 370, 501, 579, 805, 1011, 1067
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 2 cut(s) 528, 585
Van91I CCANNNNNTGG 2 cut(s) 148, 876
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 1 cut(s) 730
XceI RCATGY 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XmiI GTMKAC 2 cut(s) 395, 479
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 2 cut(s) 416, 855
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.