Rw7G012510

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000675
Physical Location & Seq
Forward (+)
11856753 .. 11879383
22631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G012510.1

Sequence Viewer

Length: 1107 bp
ATGACTACCTTGGAAATCATCATCACCTTCTCCATCTTGTTTTTCCTTTTTCCGAATCAAACATCAGCAACCTGTCATGAAACAAGATGCAAGGCCAAGGAACCATGGGTACGATTCCCTTTTCAACTACTAAGAGAAGGCCAAGACTTGGATTGTGGCTATGATCGAGGATTTGATCTCTCCTGCAGCAACCACCATAAGCGAACTATCCTCACTCTCCCATCTTCGAGAGACTTCATCGTGAAGGACATCAACTACATCGACCATATCATATGGATCGACGATCCCGGAAGCTGCTTCCCTAAGAGGCTCATGGACCATGACTTCAGTCTTGTGGACTCACCCTTCTCATACTTTTACGGCCTCGAAAGCTACGTGTTCCTCAACTGTTCGTCGACGTCCCGAGAAGAGATACTAGCACCGTCTTCACCAATTGATTGCCTCAGTAGTGAGGACTACAAGGTCATAGCTGTGCAGTCTACTTGGTTCTATGCTGATCTTAATACATCAAGCACTTCATTGAGGCCACTGGCTCAGACATCGTTATCCCGTCTTTGTTCCGTGATCTCCACAGCTATGATTCCACTGTCAGGTTCATTTGACGGGTGGGATCTTAACTTTGGTGTTCGGTTGGCATGGGACATGCCCGATTGTCGTCTATGCGAAGCAAGCGGGAGATTATGTGGGTTTGATGTTGCTACAACTTCGCAAGTTGTTGTGTGCTCTGGACTTTCAAATTCAGGTCTCTCAAATGCCAAAAAGTTCCTGTTACTAATAAGCGTGAGAGTTCCAGGGCTAGTGTGCATCACTATGTTCGCACTTTATATTTACACCTTAATCAGGGACCGTGGTCTCATCAATGAGCCCATCACAGAATTATCCAGTGTGACCATTCCACAGATTCATGTCGTTATAACATGCCTTAATGATCTAACCATTGAATCATATCTGAAGACTCAACTTGGCGAGAAATGGGAATTTCCAAGAAACAATACGAAGTTACGTACCATACCTGACTGCAACCATTATTTTCATGCTAGTTGCATATACGTGTGGCTCAGAAAGAATCCGACGTGCCCTCTGTGTCGACATCCGCCAGAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

368

Amino Acids

41.69

Weight (kDa)

5.85

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 95 1.1e-13 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 333 - 363 3.4e-09 Ring finger domain
zf-rbx1 PF12678 337 - 363 2.6e-09 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 337 - 362 3.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 914
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 148
AccI GTMKAC 3 cut(s) 395, 479, 1087
AciI CCGC 2 cut(s) 672, 1094
AclWI GGATC 3 cut(s) 278, 284, 618
AcsI RAATTY 2 cut(s) 736, 977
AcuI CTGAAG 2 cut(s) 310, 971
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 2 cut(s) 111, 1006
AfiI CCNNNNNNNGG 4 cut(s) 148, 590, 840, 1100
AflIII ACRYGT 2 cut(s) 375, 1050
AgsI TTSAA 3 cut(s) 125, 735, 941
AjiI CACGTC 1 cut(s) 1074
AjnI CCWGG 1 cut(s) 790
AluBI AGCT 4 cut(s) 294, 372, 470, 575
AluI AGCT 4 cut(s) 294, 372, 470, 575
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 3 cut(s) 225, 749, 857
AlwI GGATC 3 cut(s) 278, 284, 618
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 4 cut(s) 93, 139, 361, 524
ApeKI GCWGC 2 cut(s) 186, 294
ApoI RAATTY 2 cut(s) 736, 977
AspS9I GGNCC 2 cut(s) 316, 844
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 3 cut(s) 16, 333, 420
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 2 cut(s) 316, 844
BaeGI GKGCMC 1 cut(s) 1079
BanII GRGCYC 1 cut(s) 867
BbsI GAAGAC 2 cut(s) 417, 959
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 2 cut(s) 198, 281
BccI CCATC 3 cut(s) 41, 229, 875
BceAI ACGGC 1 cut(s) 376
BciT130I CCWGG 1 cut(s) 792
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 3 cut(s) 225, 749, 857
BfaI CTAG 4 cut(s) 416, 797, 1038, 1105
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 2 cut(s) 187, 295
BlsI GCNGC 2 cut(s) 188, 296
Bme1390I CCNGG 2 cut(s) 288, 792
Bme18I GGWCC 2 cut(s) 316, 844
BmeT110I CYCGRG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 1074
BmgT120I GGNCC 2 cut(s) 316, 844
BmiI GGNNCC 2 cut(s) 102, 845
BmrFI CCNGG 2 cut(s) 288, 792
BmsI GCATC 2 cut(s) 77, 813
BoxI GACNNNNGTC 2 cut(s) 327, 849
BpiI GAAGAC 2 cut(s) 417, 959
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 3 cut(s) 376, 1004, 1051
BsaHI GRCGYC 1 cut(s) 398
BsaI GGTCTC 2 cut(s) 749, 857
BsaJI CCNNGG 5 cut(s) 9, 96, 104, 791, 847
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 590, 840, 1100
Bse1I ACTGG 2 cut(s) 534, 882
BseBI CCWGG 1 cut(s) 792
BseDI CCNNGG 5 cut(s) 9, 96, 104, 791, 847
BseGI GGATG 1 cut(s) 1090
BseLI CCNNNNNNNGG 4 cut(s) 148, 590, 840, 1100
BseMII CTCAG 3 cut(s) 457, 548, 1072
BseNI ACTGG 2 cut(s) 534, 882
BseSI GKGCMC 1 cut(s) 1079
BseXI GCAGC 2 cut(s) 198, 281
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 4 cut(s) 95, 141, 363, 526
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 3 cut(s) 385, 653, 857
BslI CCNNNNNNNGG 4 cut(s) 148, 590, 840, 1100
BsmAI GTCTC 3 cut(s) 225, 749, 857
BsmFI GGGAC 3 cut(s) 385, 653, 857
BsnI GGCC 4 cut(s) 95, 141, 363, 526
Bso31I GGTCTC 2 cut(s) 749, 857
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 3 cut(s) 725, 867, 1079
Bsp143I GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
Bsp19I CCATGG 1 cut(s) 104
BspACI CCGC 2 cut(s) 672, 1094
BspANI GGCC 4 cut(s) 95, 141, 363, 526
BspCNI CTCAG 3 cut(s) 456, 547, 1071
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 102, 845
BspMAI CTGCAG 1 cut(s) 188
BspPI GGATC 3 cut(s) 278, 284, 618
BspTNI GGTCTC 2 cut(s) 749, 857
BsrI ACTGG 2 cut(s) 534, 882
BssECI CCNNGG 5 cut(s) 9, 96, 104, 791, 847
BssMI GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 3 cut(s) 9, 96, 104
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 4 cut(s) 389, 423, 588, 848
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 398
BstBAI YACGTR 3 cut(s) 376, 1004, 1051
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 5 cut(s) 131, 303, 443, 534, 1058
BstDSI CCRYGG 2 cut(s) 104, 847
BstENI CCTNNNNNAGG 1 cut(s) 838
BstF5I GGATG 1 cut(s) 1090
BstKTI GATC 8 cut(s) 166, 178, 279, 286, 499, 567, 613, 931
BstMAI GTCTC 3 cut(s) 225, 749, 857
BstMBI GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
BstMWI GCNNNNNNNGC 2 cut(s) 369, 669
BstNI CCWGG 1 cut(s) 792
BstNSI RCATGY 2 cut(s) 646, 921
BstPAI GACNNNNGTC 2 cut(s) 327, 849
BstSCI CCNGG 2 cut(s) 286, 790
BstSFI CTRYAG 1 cut(s) 184
BstSLI GKGCMC 1 cut(s) 1079
BstSNI TACGTA 1 cut(s) 1004
BstV1I GCAGC 2 cut(s) 198, 281
BstV2I GAAGAC 2 cut(s) 417, 959
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 4 cut(s) 95, 141, 363, 526
BtgI CCRYGG 2 cut(s) 104, 847
BtrI CACGTC 1 cut(s) 1074
BtsCI GGATG 1 cut(s) 1090
BtsIMutI CAGTG 3 cut(s) 527, 584, 889
Cac8I GCNNGC 1 cut(s) 670
CciI TCATGA 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 316, 844
Csp6I GTAC 2 cut(s) 110, 1005
CviAII CATG 9 cut(s) 77, 105, 313, 320, 636, 643, 905, 918, 1034
CviQI GTAC 2 cut(s) 110, 1005
DdeI CTNAG 5 cut(s) 131, 303, 443, 534, 1058
DpnI GATC 8 cut(s) 165, 177, 278, 285, 498, 566, 612, 930
DpnII GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 1 cut(s) 1083
Eco105I TACGTA 1 cut(s) 1004
Eco130I CCWWGG 3 cut(s) 9, 96, 104
Eco24I GRGCYC 1 cut(s) 867
Eco31I GGTCTC 2 cut(s) 749, 857
Eco47I GGWCC 2 cut(s) 316, 844
Eco57I CTGAAG 2 cut(s) 310, 971
Eco88I CYCGRG 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 838
EcoRII CCWGG 1 cut(s) 790
EcoT14I CCWWGG 3 cut(s) 9, 96, 104
EcoT38I GRGCYC 1 cut(s) 867
ErhI CCWWGG 3 cut(s) 9, 96, 104
FaeI CATG 9 cut(s) 80, 108, 316, 323, 639, 646, 908, 921, 1037
FaqI GGGAC 3 cut(s) 385, 653, 857
FatI CATG 9 cut(s) 76, 104, 312, 319, 635, 642, 904, 917, 1033
FauI CCCGC 1 cut(s) 665
FauNDI CATATG 1 cut(s) 272
FblI GTMKAC 3 cut(s) 395, 479, 1087
Fnu4HI GCNGC 2 cut(s) 187, 295
FokI GGATG 1 cut(s) 1077
FriOI GRGCYC 1 cut(s) 867
Fsp4HI GCNGC 2 cut(s) 187, 295
FspBI CTAG 4 cut(s) 416, 797, 1038, 1105
GluI GCNGC 2 cut(s) 187, 295
HaeIII GGCC 4 cut(s) 95, 141, 363, 526
HapII CCGG 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 9 cut(s) 80, 108, 316, 323, 639, 646, 908, 921, 1037
HincII GTYRAC 2 cut(s) 396, 1088
HindII GTYRAC 2 cut(s) 396, 1088
HinfI GANTC 8 cut(s) 55, 114, 338, 580, 901, 941, 955, 1066
HpaII CCGG 1 cut(s) 288
HphI GGTGA 3 cut(s) 16, 333, 420
Hpy166II GTNNAC 4 cut(s) 337, 396, 480, 1088
Hpy188I TCNGA 5 cut(s) 54, 537, 951, 1061, 1071
Hpy188III TCNNGA 5 cut(s) 77, 228, 241, 402, 726
Hpy8I GTNNAC 4 cut(s) 337, 396, 480, 1088
Hpy99I CGWCG 4 cut(s) 284, 397, 400, 1075
HpyAV CCTTC 4 cut(s) 37, 131, 238, 355
HpyCH4III ACNGT 4 cut(s) 389, 423, 588, 848
HpyCH4IV ACGT 5 cut(s) 375, 398, 1003, 1050, 1073
HpyCH4V TGCA 6 cut(s) 90, 186, 475, 804, 1020, 1044
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 669
HpyF3I CTNAG 5 cut(s) 131, 303, 443, 534, 1058
HpySE526I ACGT 5 cut(s) 375, 398, 1003, 1050, 1073
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 9 cut(s) 80, 108, 316, 323, 639, 646, 908, 921, 1037
Kzo9I GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
Lsp1109I GCAGC 2 cut(s) 198, 281
LweI GCATC 2 cut(s) 77, 813
MaeI CTAG 4 cut(s) 416, 797, 1038, 1105
MaeII ACGT 5 cut(s) 375, 398, 1003, 1050, 1073
MaeIII GTNAC 3 cut(s) 768, 886, 999
MalI GATC 8 cut(s) 165, 177, 278, 285, 498, 566, 612, 930
MboI GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
MboII GAAGA 4 cut(s) 216, 417, 419, 964
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 3 cut(s) 725, 867, 1079
MluCI AATT 4 cut(s) 432, 736, 875, 977
MlyI GAGTC 2 cut(s) 332, 949
MmeI TCCRAC 1 cut(s) 1094
MseI TTAA 4 cut(s) 501, 615, 836, 924
MslI CAYNNNNRTG 4 cut(s) 470, 575, 809, 1049
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 288, 792
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 792
MwoI GCNNNNNNNGC 2 cut(s) 369, 669
NciI CCSGG 1 cut(s) 288
NcoI CCATGG 1 cut(s) 104
NdeI CATATG 1 cut(s) 272
NdeII GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
NlaIII CATG 9 cut(s) 80, 108, 316, 323, 639, 646, 908, 921, 1037
NlaIV GGNNCC 2 cut(s) 102, 845
NmuCI GTSAC 1 cut(s) 886
NspI RCATGY 2 cut(s) 646, 921
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PfeI GAWTC 6 cut(s) 55, 114, 580, 901, 941, 1066
PflMI CCANNNNNTGG 1 cut(s) 148
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 2 cut(s) 188, 296
PleI GAGTC 2 cut(s) 332, 949
PpsI GAGTC 2 cut(s) 332, 949
Ppu21I YACGTR 3 cut(s) 376, 1004, 1051
PshAI GACNNNNGTC 2 cut(s) 327, 849
PsiI TTATAA 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 790
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 2 cut(s) 102, 845
PspPI GGNCC 2 cut(s) 316, 844
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 2 cut(s) 111, 1006
RsaNI GTAC 2 cut(s) 110, 1005
RseI CAYNNNNRTG 4 cut(s) 470, 575, 809, 1049
SalI GTCGAC 2 cut(s) 394, 1086
SaqAI TTAA 4 cut(s) 501, 615, 836, 924
SatI GCNGC 2 cut(s) 187, 295
Sau3AI GATC 8 cut(s) 163, 175, 276, 283, 496, 564, 610, 928
Sau96I GGNCC 2 cut(s) 316, 844
SchI GAGTC 2 cut(s) 332, 949
ScrFI CCNGG 2 cut(s) 288, 792
SduI GDGCHC 3 cut(s) 725, 867, 1079
SfaNI GCATC 2 cut(s) 77, 813
SfcI CTRYAG 1 cut(s) 184
SgrDI CGTCGACG 1 cut(s) 394
SinI GGWCC 2 cut(s) 316, 844
SmiMI CAYNNNNRTG 4 cut(s) 470, 575, 809, 1049
SnaBI TACGTA 1 cut(s) 1004
Sse9I AATT 4 cut(s) 432, 736, 875, 977
SsiI CCGC 2 cut(s) 672, 1094
SspMI CTAG 4 cut(s) 416, 797, 1038, 1105
StyD4I CCNGG 2 cut(s) 286, 790
StyI CCWWGG 3 cut(s) 9, 96, 104
TaaI ACNGT 4 cut(s) 389, 423, 588, 848
TaiI ACGT 5 cut(s) 378, 401, 1006, 1053, 1076
TaqI TCGA 7 cut(s) 166, 227, 261, 279, 366, 395, 1087
TasI AATT 4 cut(s) 432, 736, 875, 977
TfiI GAWTC 6 cut(s) 55, 114, 580, 901, 941, 1066
Tru1I TTAA 4 cut(s) 501, 615, 836, 924
Tru9I TTAA 4 cut(s) 501, 615, 836, 924
TscAI CASTG 3 cut(s) 534, 591, 889
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 2 cut(s) 186, 294
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 6 cut(s) 93, 226, 507, 585, 893, 1022
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 3 cut(s) 534, 591, 889
Van91I CCANNNNNTGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 316, 844
XagI CCTNNNNNAGG 1 cut(s) 838
XapI RAATTY 2 cut(s) 736, 977
XceI RCATGY 2 cut(s) 646, 921
XmiI GTMKAC 3 cut(s) 395, 479, 1087
XspI CTAG 4 cut(s) 416, 797, 1038, 1105
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.