Rh7CG150600

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000675
Physical Location & Seq
Forward (+)
12359022 .. 12389185
30164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG150600.1

Sequence Viewer

Length: 1194 bp
ATGGCTATAATTATCAAAATTATAGTTTGGAGCAACTTAATATCGGGCGAGGTTTACTACCAAACCTTCGGCCAAAATATGAATGGCCAATTGAGCCATGGTAATTGGCAAGGTTATAATTCTAATATAGATATGTATGAGCCAACTAACAATTCTATCGGCCAAAATTGTTACTCTCAATTGGCCGATAAAAATTATCATGAGCCGAGGGACCGTGGTCGCATCAATGAGCCCATCACAGAATTATCCAGTGTGACCATTCCACAGATTCATGTCGTTATAACAGGCCTTAATGATCTAACCATTGAATCATATCCAAAGACTCAACTTGGCGAGAAATGGGAATTTCCAAGTCCCGAGGACAGCACTTGCCCAATATGTTTGTGCAAGTACCAACCCGAGGAAACTATAAGGACAATACCTGAATGCAATCATCATTTTCATGCCAATTGCATTGATGAATGGCTCAGAAAGAATCCTACGTGCCCAGTTTGTCAAAAAGTTTCGCAAGAGGATAACAAAACAACTAAAGATGGAAAGCGGGCTGCATCCATCTCTTGCCTCAGCAGTGACTACTACAAGCTAGGTCCTTCTTGTGCCTACATCAGATTGGCCAACGTCATGTGGAGAGATCTTAATGACGTCCTTGATATTCTTTTGTCATGGGACAAGCCTGATTGTCGTTCTTGTGAAGCAGGAGGTCTACGTTGTAAGTTTGAGAATGCTAAAGGTTCGCAAGTTCGGTGCTCCGGTTCTTCACACATCAGTCTCTCAAGATCAGCAAAGTATGGCATAATGATAGGTGTCGGAATACCAGGGATATTGTGCATTCTTGGGCTTGGGCTTTATGTTTGTGACAGGGTAGGGAGAGCGCGAGATGGACTCCACCGGAGACAGTCCGTCACAGAATTGTCCACCGCAGTTGATCAACAAGTACAACCTCCCCGAGTCATAGTTGGGCTTGATGGTCCAACAATTGAGTCGTATCCCAAGATCCAACTAGATGAGAGCTTGGAGTTGCCCGAGCCCGATGATAAGACTAGTGCGATATGTTTGTGCGAGTCAGCCCAAGGAAACAATACGAAGTTACGAACCATACCTGACTGCAACCATTATTTTCATGCCAGTTGCATACACGAGTGGCTCAGAAAGAATCCGACGTGCCCTCTGTGTCGACATCCGCCAGAGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

397

Amino Acids

44.52

Weight (kDa)

6.07

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 123 - 166 7.6e-14 Ring finger domain
zf-rbx1 PF12678 123 - 166 1.8e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 124 - 165 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 124 - 152 2.6e-07 RING-like zinc finger
zf-C3HC4 PF00097 124 - 165 3.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 349 - 392 4.2e-11 Ring finger domain
zf-rbx1 PF12678 351 - 392 1.2e-09 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 366 - 391 8.8e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 281
AatII GACGTC 1 cut(s) 645
AccI GTMKAC 2 cut(s) 703, 1174
AccII CGCG 1 cut(s) 874
AciI CCGC 3 cut(s) 541, 918, 1181
AclWI GGATC 1 cut(s) 988
AcoI YGGCCR 5 cut(s) 70, 85, 160, 183, 612
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 642
AfaI GTAC 2 cut(s) 392, 936
AfiI CCNNNNNNNGG 2 cut(s) 400, 1187
AgsI TTSAA 1 cut(s) 308
AhdI GACNNNNNGTC 1 cut(s) 899
AhlI ACTAGT 1 cut(s) 1040
AjiI CACGTC 1 cut(s) 1161
AjnI CCWGG 1 cut(s) 814
AjuI GAANNNNNNNTTGG 2 cut(s) 136, 168
AluBI AGCT 2 cut(s) 583, 1011
AluI AGCT 2 cut(s) 583, 1011
Alw21I GWGCWC 1 cut(s) 749
Alw26I GTCTC 2 cut(s) 773, 886
AlwI GGATC 1 cut(s) 988
Ama87I CYCGRG 4 cut(s) 356, 398, 945, 1022
AoxI GGCC 6 cut(s) 70, 85, 160, 183, 286, 612
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 874
AspS9I GGNCC 3 cut(s) 211, 587, 968
AvaI CYCGRG 4 cut(s) 356, 398, 945, 1022
AvaII GGWCC 3 cut(s) 211, 587, 968
BaeGI GKGCMC 2 cut(s) 488, 1166
BalI TGGCCA 2 cut(s) 87, 614
BanII GRGCYC 2 cut(s) 234, 1029
BauI CACGAG 1 cut(s) 1136
Bbv12I GWGCWC 1 cut(s) 749
BbvCI CCTCAGC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 532
BccI CCATC 5 cut(s) 242, 527, 560, 872, 959
BciT130I CCWGG 1 cut(s) 816
BciVI GTATCC 1 cut(s) 996
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 2 cut(s) 773, 886
BcuI ACTAGT 1 cut(s) 1040
BfaI CTAG 4 cut(s) 584, 1001, 1041, 1192
BfuI GTATCC 1 cut(s) 996
BglII AGATCT 1 cut(s) 631
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 816
Bme18I GGWCC 3 cut(s) 211, 587, 968
BmeRI GACNNNNNGTC 1 cut(s) 899
BmeT110I CYCGRG 4 cut(s) 356, 398, 945, 1022
BmgBI CACGTC 1 cut(s) 1161
BmgT120I GGNCC 3 cut(s) 211, 587, 968
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 816
BmrI ACTGGG 1 cut(s) 482
BmsI GCATC 2 cut(s) 231, 557
BmuI ACTGGG 1 cut(s) 482
BoxI GACNNNNGTC 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 867, 899
Bpu10I CCTNAGC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 642
BsaJI CCNNGG 7 cut(s) 97, 206, 214, 357, 399, 815, 1069
BsaWI WCCGGW 2 cut(s) 749, 888
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 1187
Bse1I ACTGG 3 cut(s) 249, 488, 1125
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 7 cut(s) 97, 206, 214, 357, 399, 815, 1069
BseGI GGATG 2 cut(s) 548, 1177
BseLI CCNNNNNNNGG 2 cut(s) 400, 1187
BseMII CTCAG 3 cut(s) 481, 577, 1159
BseNI ACTGG 3 cut(s) 249, 488, 1125
BseSI GKGCMC 2 cut(s) 488, 1166
BseXI GCAGC 1 cut(s) 532
Bsh1236I CGCG 1 cut(s) 874
BshFI GGCC 6 cut(s) 72, 87, 162, 185, 288, 614
BsiHKAI GWGCWC 1 cut(s) 749
BsiHKCI CYCGRG 4 cut(s) 356, 398, 945, 1022
BsiSI CCGG 2 cut(s) 750, 889
BslFI GGGAC 3 cut(s) 224, 339, 680
BslI CCNNNNNNNGG 2 cut(s) 400, 1187
BsmAI GTCTC 2 cut(s) 773, 886
BsmFI GGGAC 3 cut(s) 224, 339, 680
BsmI GAATGC 3 cut(s) 431, 727, 828
BsnI GGCC 6 cut(s) 72, 87, 162, 185, 288, 614
BsoBI CYCGRG 4 cut(s) 356, 398, 945, 1022
Bsp1286I GDGCHC 5 cut(s) 234, 488, 749, 1029, 1166
Bsp143I GATC 5 cut(s) 295, 631, 776, 925, 993
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 3 cut(s) 541, 918, 1181
BspANI GGCC 6 cut(s) 72, 87, 162, 185, 288, 614
BspCNI CTCAG 3 cut(s) 480, 576, 1158
BspFNI CGCG 1 cut(s) 874
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 212
BspPI GGATC 1 cut(s) 988
BsrI ACTGG 3 cut(s) 249, 488, 1125
BssECI CCNNGG 7 cut(s) 97, 206, 214, 357, 399, 815, 1069
BssMI GATC 5 cut(s) 295, 631, 776, 925, 993
BssNI GRCGYC 1 cut(s) 642
BssSI CACGAG 1 cut(s) 1136
BssT1I CCWWGG 2 cut(s) 97, 1069
Bst2BI CACGAG 1 cut(s) 1136
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 2 cut(s) 215, 897
BstACI GRCGYC 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 3 cut(s) 467, 563, 1145
BstDSI CCRYGG 2 cut(s) 97, 214
BstF5I GGATG 2 cut(s) 548, 1177
BstFNI CGCG 1 cut(s) 874
BstHHI GCGC 1 cut(s) 874
BstKTI GATC 5 cut(s) 298, 634, 779, 928, 996
BstMAI GTCTC 2 cut(s) 773, 886
BstMBI GATC 5 cut(s) 295, 631, 776, 925, 993
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNI CCWGG 1 cut(s) 816
BstPAI GACNNNNGTC 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 814
BstSLI GKGCMC 2 cut(s) 488, 1166
BstUI CGCG 1 cut(s) 874
BstV1I GCAGC 1 cut(s) 532
BstX2I RGATCY 2 cut(s) 631, 993
BstYI RGATCY 2 cut(s) 631, 993
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 6 cut(s) 72, 87, 162, 185, 288, 614
BtgI CCRYGG 2 cut(s) 97, 214
BtrI CACGTC 1 cut(s) 1161
BtsCI GGATG 2 cut(s) 548, 1177
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 2 cut(s) 256, 574
Cac8I GCNNGC 1 cut(s) 543
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 874
Cfr13I GGNCC 3 cut(s) 211, 587, 968
Csp6I GTAC 2 cut(s) 391, 935
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 7 cut(s) 98, 200, 272, 443, 622, 663, 1121
CviQI GTAC 2 cut(s) 391, 935
DdeI CTNAG 3 cut(s) 467, 563, 1145
DpnI GATC 5 cut(s) 297, 633, 778, 927, 995
DpnII GATC 5 cut(s) 295, 631, 776, 925, 993
DriI GACNNNNNGTC 1 cut(s) 899
EaeI YGGCCR 5 cut(s) 70, 85, 160, 183, 612
Eam1105I GACNNNNNGTC 1 cut(s) 899
EciI GGCGGA 1 cut(s) 1170
Eco130I CCWWGG 2 cut(s) 97, 1069
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 2 cut(s) 234, 1029
Eco47I GGWCC 3 cut(s) 211, 587, 968
Eco88I CYCGRG 4 cut(s) 356, 398, 945, 1022
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 97, 1069
EcoT38I GRGCYC 2 cut(s) 234, 1029
ErhI CCWWGG 2 cut(s) 97, 1069
FaeI CATG 7 cut(s) 101, 203, 275, 446, 625, 666, 1124
FaqI GGGAC 3 cut(s) 224, 339, 680
FatI CATG 7 cut(s) 97, 199, 271, 442, 621, 662, 1120
FauI CCCGC 1 cut(s) 534
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 2 cut(s) 703, 1174
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 2 cut(s) 535, 1164
FriOI GRGCYC 2 cut(s) 234, 1029
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 4 cut(s) 584, 1001, 1041, 1192
GlaI GCGC 1 cut(s) 873
GluI GCNGC 1 cut(s) 546
HaeIII GGCC 6 cut(s) 72, 87, 162, 185, 288, 614
HapII CCGG 2 cut(s) 750, 889
HhaI GCGC 1 cut(s) 874
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 7 cut(s) 101, 203, 275, 446, 625, 666, 1124
Hin6I GCGC 1 cut(s) 872
HinP1I GCGC 1 cut(s) 872
HincII GTYRAC 1 cut(s) 1175
HindII GTYRAC 1 cut(s) 1175
HinfI GANTC 9 cut(s) 268, 308, 322, 475, 882, 948, 980, 1061, 1153
HpaII CCGG 2 cut(s) 750, 889
Hpy166II GTNNAC 4 cut(s) 55, 704, 915, 1175
Hpy188I TCNGA 5 cut(s) 470, 608, 809, 1148, 1158
Hpy188III TCNNGA 3 cut(s) 200, 356, 774
Hpy8I GTNNAC 4 cut(s) 55, 704, 915, 1175
Hpy99I CGWCG 1 cut(s) 1162
HpyAV CCTTC 2 cut(s) 76, 600
HpyCH4III ACNGT 2 cut(s) 215, 897
HpyCH4IV ACGT 5 cut(s) 482, 618, 642, 706, 1160
HpyCH4V TGCA 7 cut(s) 387, 429, 453, 548, 828, 1107, 1131
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpyF3I CTNAG 3 cut(s) 467, 563, 1145
HpySE526I ACGT 5 cut(s) 482, 618, 642, 706, 1160
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 7 cut(s) 101, 203, 275, 446, 625, 666, 1124
HspAI GCGC 1 cut(s) 872
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 5 cut(s) 295, 631, 776, 925, 993
LmnI GCTCC 2 cut(s) 30, 752
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 2 cut(s) 231, 557
MaeI CTAG 4 cut(s) 584, 1001, 1041, 1192
MaeII ACGT 5 cut(s) 482, 618, 642, 706, 1160
MaeIII GTNAC 6 cut(s) 170, 253, 569, 854, 901, 1086
MalI GATC 5 cut(s) 297, 633, 778, 927, 995
MboI GATC 5 cut(s) 295, 631, 776, 925, 993
MboII GAAGA 1 cut(s) 747
MfeI CAATTG 4 cut(s) 89, 179, 448, 975
MflI RGATCY 2 cut(s) 631, 993
MhlI GDGCHC 5 cut(s) 234, 488, 749, 1029, 1166
MlsI TGGCCA 2 cut(s) 87, 614
MluNI TGGCCA 2 cut(s) 87, 614
MlyI GAGTC 5 cut(s) 316, 876, 957, 989, 1070
MmeI TCCRAC 4 cut(s) 787, 995, 1021, 1181
Mox20I TGGCCA 2 cut(s) 87, 614
MscI TGGCCA 2 cut(s) 87, 614
MseI TTAA 3 cut(s) 38, 291, 636
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 2 cut(s) 87, 614
MspI CCGG 2 cut(s) 750, 889
MspR9I CCNGG 1 cut(s) 816
MunI CAATTG 4 cut(s) 89, 179, 448, 975
Mva1269I GAATGC 3 cut(s) 431, 727, 828
MvaI CCWGG 1 cut(s) 816
MvnI CGCG 1 cut(s) 874
MwoI GCNNNNNNNGC 1 cut(s) 93
NcoI CCATGG 1 cut(s) 97
NdeII GATC 5 cut(s) 295, 631, 776, 925, 993
NlaIII CATG 7 cut(s) 101, 203, 275, 446, 625, 666, 1124
NlaIV GGNNCC 1 cut(s) 212
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 4 cut(s) 253, 569, 854, 901
PagI TCATGA 1 cut(s) 199
PceI AGGCCT 1 cut(s) 288
PctI GAATGC 3 cut(s) 431, 727, 828
PfeI GAWTC 4 cut(s) 268, 308, 475, 1153
PkrI GCNGC 1 cut(s) 547
PleI GAGTC 5 cut(s) 316, 876, 956, 988, 1069
PpsI GAGTC 5 cut(s) 316, 876, 956, 988, 1069
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 587
PshAI GACNNNNGTC 1 cut(s) 216
PsiI TTATAA 2 cut(s) 117, 281
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 3 cut(s) 211, 587, 968
PspPPI RGGWCCY 1 cut(s) 587
PsuI RGATCY 2 cut(s) 631, 993
RsaI GTAC 2 cut(s) 392, 936
RsaNI GTAC 2 cut(s) 391, 935
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 1173
SaqAI TTAA 3 cut(s) 38, 291, 636
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 295, 631, 776, 925, 993
Sau96I GGNCC 3 cut(s) 211, 587, 968
SchI GAGTC 5 cut(s) 316, 876, 957, 989, 1070
ScrFI CCNGG 1 cut(s) 816
SduI GDGCHC 5 cut(s) 234, 488, 749, 1029, 1166
SfaNI GCATC 2 cut(s) 231, 557
SinI GGWCC 3 cut(s) 211, 587, 968
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
SpeI ACTAGT 1 cut(s) 1040
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 3 cut(s) 541, 918, 1181
SspMI CTAG 4 cut(s) 584, 1001, 1041, 1192
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 814
StyI CCWWGG 2 cut(s) 97, 1069
TaaI ACNGT 2 cut(s) 215, 897
TaiI ACGT 5 cut(s) 485, 621, 645, 709, 1163
TaqI TCGA 1 cut(s) 1174
TatI WGTACW 1 cut(s) 934
TfiI GAWTC 4 cut(s) 268, 308, 475, 1153
Tru1I TTAA 3 cut(s) 38, 291, 636
Tru9I TTAA 3 cut(s) 38, 291, 636
TscAI CASTG 2 cut(s) 256, 574
TseFI GTSAC 4 cut(s) 253, 569, 854, 901
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 4 cut(s) 253, 569, 854, 901
TspDTI ATGAA 5 cut(s) 95, 260, 431, 474, 1109
TspGWI ACGGA 1 cut(s) 889
TspRI CASTG 2 cut(s) 256, 574
VpaK11BI GGWCC 3 cut(s) 211, 587, 968
XapI RAATTY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 2 cut(s) 80, 95
XmiI GTMKAC 2 cut(s) 703, 1174
XspI CTAG 4 cut(s) 584, 1001, 1041, 1192
ZraI GACGTC 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.