Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000675

Physical Location & Seq

Genomic Coordinates Reverse (-)

20554655 .. 20555685

Gene Structure 1031 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1128700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 774 bp

ATGATACAAGTGGTATCGATCAGCGACCCCGATAACTGTCTCCCTAAACGGTTTCTCCACCATGAAACTAGTCTTGTGGAAAATTCACCCTTCCTCCACCGTACTTTCAGCAGCCTGGTGAACTACACGTTCTTCAACTGTTCCTCCTCTGAAGTAGGCAGCATGCCTTCACGCATCATATCTTGCCTCAGCACTGAGTACTTCAAGGTCATTGCTTTGCGTACAAGTTTGCTATTGCCACCCTATCCAATTCCATTGCCCCTTTGCTCTGTGATTTCGACAACTTTGGCTCCGAGTTTATTCGATTGGGATAACCATGACATCGAGTTGACATGGAAGGTGCCTGATTGTCTTGGAGTCTCTTCAGATCCCTCAAAAGCTAGAAAGCACGGCATAATGATGGGTGTGGGAATTCCTGTGCTTTTGTTAAGCATTTGGCTTGGACTTCATTGTTGTGGCAGAATGACGGAGGTCAGTGGACGGAACCAGCCCATCGTAGAATTATCTACCGTAACCAATCGGCAACCTCGCGCAATCACAACCGGCCTTGACGGTCCGACCATCGAATCATATCCCAAGACTCAACTTGGTGAGAGATTGGAATTGCCCAAGCCTAATGACAACACTTGCCCAATATGCTTGTGTGAGTACCAGTCCATGGAAACATTAAGGACCATACCTGATTGCGAACACTATTTCCATGCTAATTGTATAGATGAATGGCTTAAGTTGAACGCAACGTGCCCGCTTTGTCGAAATCCACAGGAGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

258

Amino Acids

28.85

Weight (kDa)

5.89

Isoelectric Point (pI)

41.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	208 - 252	3.6e-13	Ring finger domain
zf-rbx1	PF12678	208 - 252	3.7e-08	RING-H2 zinc finger domain
zf-RING_11	PF17123	210 - 238	4.3e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	210 - 251	1.3e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000675)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g04540 FvH4_5g04550 FvH4_5g04551 FvH4_5g04552 FvH4_5g04554 FvH4_5g29040
malus_domestica	MD06G1106600.v1.1 MD14G1128600.v1.1 MD14G1128700.v1.1
prunus_persica	Prupe.5G118700_v2.0.a1 Prupe.5G118800_v2.0.a1 Prupe.5G119000_v2.0.a1 Prupe.5G119300_v2.0.a1 Prupe.5G119500_v2.0.a1 Prupe.5G119600_v2.0.a1 Prupe.5G119700_v2.0.a1 Prupe.5G119800_v2.0.a1 Prupe.5G119900_v2.0.a1
pyrus_communis	pycom06g10070 pycom06g10090
rosa_chinensis	RchiOBHm_Chr7g0194821 RchiOBHm_Chr7g0194841 RchiOBHm_Chr7g0194851 RchiOBHm_Chr7g0194861 RchiOBHm_Chr7g0194871 RchiOBHm_Chr7g0226371
rosa_laevigata	RLG00000001745 RLG00000004159 RLG00000004160 RLG00000004162 RLG00000004163 RLG00000004166 RLG00000013439
rosa_multiflora	Rmu_co8140360.1_g000001 Rmu_co8197174.1_g000001 Rmu_co8260763.1_g000001 Rmu_co8287607.1_g000001 Rmu_co8453499.1_g000001 Rmu_sc0011834.1_g000004 Rmu_sc0017428.1_g000003 Rmu_sc0040407.1_g000001
rosa_roxburghii	Rroxscaffold_3G00260910 Rroxscaffold_3G00260920
rosa_rugosa	Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0236700
rosa_samantha	Rh7AG146400 Rh7AG146500 Rh7AG146600 Rh7AG383700 Rh7BG146300 Rh7BG146400 Rh7BG146500 Rh7BG146600 Rh7BG146900 Rh7BG147100 Rh7BG147200 Rh7BG147300 Rh7BG370800 Rh7CG150400 Rh7CG150500 Rh7CG150600 Rh7CG403300 Rh7DG147600 Rh7DG147700 Rh7DG147800 Rh7DG382100 Rh7DG482500
rosa_wichuraiana	Rw7G012490 Rw7G012500 Rw7G012510 Rw7G032160 Rw7G032220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	340
AccB7I	CCANNNNNTGG	1 cut(s)	658
AccII	CGCG	1 cut(s)	531
AciI	CCGC	1 cut(s)	746
AclWI	GGATC	1 cut(s)	362
AcsI	RAATTY	2 cut(s)	82, 411
AcuI	CTGAAG	2 cut(s)	171, 348
AfaI	GTAC	4 cut(s)	103, 200, 223, 650
AfiI	CCNNNNNNNGG	1 cut(s)	658
AflII	CTTAAG	1 cut(s)	725
AflIII	ACRYGT	1 cut(s)	126
AgsI	TTSAA	3 cut(s)	136, 205, 733
AhlI	ACTAGT	1 cut(s)	68
AjnI	CCWGG	1 cut(s)	114
AloI	GAACNNNNNNTCC	2 cut(s)	681, 713
AluBI	AGCT	1 cut(s)	380
AluI	AGCT	1 cut(s)	380
Alw26I	GTCTC	2 cut(s)	44, 364
AlwI	GGATC	1 cut(s)	362
AoxI	GGCC	1 cut(s)	544
ApeKI	GCWGC	2 cut(s)	111, 159
ApoI	RAATTY	2 cut(s)	82, 411
AspLEI	GCGC	1 cut(s)	533
AspS9I	GGNCC	2 cut(s)	554, 672
AsuHPI	GGTGA	3 cut(s)	78, 130, 602
AvaII	GGWCC	2 cut(s)	554, 672
BaeGI	GKGCMC	1 cut(s)	746
BanI	GGYRCC	1 cut(s)	340
BbvCI	CCTCAGC	1 cut(s)	188
BbvI	GCAGC	2 cut(s)	123, 171
BccI	CCATC	3 cut(s)	394, 500, 569
BceAI	ACGGC	1 cut(s)	406
BciT130I	CCWGG	1 cut(s)	116
BcoDI	GTCTC	2 cut(s)	44, 364
BcuI	ACTAGT	1 cut(s)	68
BfaI	CTAG	2 cut(s)	69, 381
BfrI	CTTAAG	1 cut(s)	725
BisI	GCNGC	2 cut(s)	112, 160
BlsI	GCNGC	2 cut(s)	113, 161
BmcAI	AGTACT	1 cut(s)	200
Bme1390I	CCNGG	1 cut(s)	116
Bme18I	GGWCC	2 cut(s)	554, 672
BmgT120I	GGNCC	2 cut(s)	554, 672
BmiI	GGNNCC	3 cut(s)	291, 342, 485
BmrFI	CCNGG	1 cut(s)	116
BmsI	GCATC	1 cut(s)	183
BoxI	GACNNNNGTC	1 cut(s)	470
Bpu10I	CCTNAGC	1 cut(s)	188
Bsa29I	ATCGAT	1 cut(s)	17
BsaJI	CCNNGG	1 cut(s)	657
BsaXI	ACNNNNNCTCC	8 cut(s)	39, 69, 78, 108, 128, 158, 274, 304
Bsc4I	CCNNNNNNNGG	1 cut(s)	658
Bse118I	RCCGGY	1 cut(s)	542
Bse1I	ACTGG	1 cut(s)	652
Bse3DI	GCAATG	2 cut(s)	210, 254
BseBI	CCWGG	1 cut(s)	116
BseCI	ATCGAT	1 cut(s)	17
BseDI	CCNNGG	1 cut(s)	657
BseLI	CCNNNNNNNGG	1 cut(s)	658
BseMI	GCAATG	2 cut(s)	210, 254
BseMII	CTCAG	2 cut(s)	186, 202
BseNI	ACTGG	1 cut(s)	652
BseRI	GAGGAG	1 cut(s)	136
BseSI	GKGCMC	1 cut(s)	746
BseXI	GCAGC	2 cut(s)	123, 171
Bsh1236I	CGCG	1 cut(s)	531
BshFI	GGCC	1 cut(s)	546
BshNI	GGYRCC	1 cut(s)	340
BshVI	ATCGAT	1 cut(s)	17
BsiSI	CCGG	1 cut(s)	543
BslI	CCNNNNNNNGG	1 cut(s)	658
BsmAI	GTCTC	2 cut(s)	44, 364
BsnI	GGCC	1 cut(s)	546
Bsp1286I	GDGCHC	1 cut(s)	746
Bsp143I	GATC	2 cut(s)	18, 367
Bsp19I	CCATGG	1 cut(s)	657
BspACI	CCGC	1 cut(s)	746
BspANI	GGCC	1 cut(s)	546
BspCNI	CTCAG	2 cut(s)	187, 201
BspDI	ATCGAT	1 cut(s)	17
BspFNI	CGCG	1 cut(s)	531
BspLI	GGNNCC	3 cut(s)	291, 342, 485
BspPI	GGATC	1 cut(s)	362
BspT107I	GGYRCC	1 cut(s)	340
BspTI	CTTAAG	1 cut(s)	725
BsrDI	GCAATG	2 cut(s)	210, 254
BsrFI	RCCGGY	1 cut(s)	542
BsrI	ACTGG	1 cut(s)	652
BssAI	RCCGGY	1 cut(s)	542
BssECI	CCNNGG	1 cut(s)	657
BssMI	GATC	2 cut(s)	18, 367
BssT1I	CCWWGG	1 cut(s)	657
Bst2UI	CCWGG	1 cut(s)	116
Bst4CI	ACNGT	6 cut(s)	38, 51, 101, 140, 511, 554
Bst6I	CTCTTC	1 cut(s)	367
BstAFI	CTTAAG	1 cut(s)	725
BstC8I	GCNNGC	2 cut(s)	164, 746
BstDEI	CTNAG	2 cut(s)	188, 195
BstDSI	CCRYGG	1 cut(s)	657
BstFNI	CGCG	1 cut(s)	531
BstHHI	GCGC	1 cut(s)	533
BstKTI	GATC	2 cut(s)	21, 370
BstMAI	GTCTC	2 cut(s)	44, 364
BstMBI	GATC	2 cut(s)	18, 367
BstMWI	GCNNNNNNNGC	1 cut(s)	636
BstNI	CCWGG	1 cut(s)	116
BstNSI	RCATGY	1 cut(s)	166
BstPAI	GACNNNNGTC	1 cut(s)	470
BstSCI	CCNGG	1 cut(s)	114
BstSLI	GKGCMC	1 cut(s)	746
BstUI	CGCG	1 cut(s)	531
BstV1I	GCAGC	2 cut(s)	123, 171
BstX2I	RGATCY	1 cut(s)	367
BstYI	RGATCY	1 cut(s)	367
Bsu15I	ATCGAT	1 cut(s)	17
BsuRI	GGCC	1 cut(s)	546
BsuTUI	ATCGAT	1 cut(s)	17
BtgI	CCRYGG	1 cut(s)	657
BtsIMutI	CAGTG	2 cut(s)	192, 481
Cac8I	GCNNGC	2 cut(s)	164, 746
CfoI	GCGC	1 cut(s)	533
Cfr10I	RCCGGY	1 cut(s)	542
Cfr13I	GGNCC	2 cut(s)	554, 672
ClaI	ATCGAT	1 cut(s)	17
CpoI	CGGWCCG	1 cut(s)	554
Csp6I	GTAC	4 cut(s)	102, 199, 222, 649
CspI	CGGWCCG	1 cut(s)	554
CviAII	CATG	6 cut(s)	62, 163, 317, 333, 658, 701
CviJI	RGCY	8 cut(s)	114, 290, 380, 439, 490, 546, 613, 724
CviKI_1	RGCY	8 cut(s)	114, 290, 380, 439, 490, 546, 613, 724
CviQI	GTAC	4 cut(s)	102, 199, 222, 649
DdeI	CTNAG	2 cut(s)	188, 195
DpnI	GATC	2 cut(s)	20, 369
DpnII	GATC	2 cut(s)	18, 367
Eam1104I	CTCTTC	1 cut(s)	367
EarI	CTCTTC	1 cut(s)	367
Eco130I	CCWWGG	1 cut(s)	657
Eco47I	GGWCC	2 cut(s)	554, 672
Eco57I	CTGAAG	2 cut(s)	171, 348
EcoRI	GAATTC	1 cut(s)	411
EcoRII	CCWGG	1 cut(s)	114
EcoT14I	CCWWGG	1 cut(s)	657
ErhI	CCWWGG	1 cut(s)	657
FaeI	CATG	6 cut(s)	65, 166, 320, 336, 661, 704
FatI	CATG	6 cut(s)	61, 162, 316, 332, 657, 700
FauI	CCCGC	1 cut(s)	753
Fnu4HI	GCNGC	2 cut(s)	112, 160
Fsp4HI	GCNGC	2 cut(s)	112, 160
FspBI	CTAG	2 cut(s)	69, 381
GlaI	GCGC	1 cut(s)	532
GluI	GCNGC	2 cut(s)	112, 160
HaeIII	GGCC	1 cut(s)	546
HapII	CCGG	1 cut(s)	543
HhaI	GCGC	1 cut(s)	533
Hin1II	CATG	6 cut(s)	65, 166, 320, 336, 661, 704
Hin6I	GCGC	1 cut(s)	531
HinP1I	GCGC	1 cut(s)	531
HincII	GTYRAC	1 cut(s)	330
HindII	GTYRAC	1 cut(s)	330
HinfI	GANTC	3 cut(s)	357, 566, 580
HpaII	CCGG	1 cut(s)	543
HphI	GGTGA	3 cut(s)	78, 130, 602
Hpy166II	GTNNAC	3 cut(s)	121, 330, 479
Hpy188I	TCNGA	4 cut(s)	151, 294, 367, 558
Hpy8I	GTNNAC	3 cut(s)	121, 330, 479
HpyAV	CCTTC	3 cut(s)	100, 177, 331
HpyCH4III	ACNGT	6 cut(s)	38, 51, 101, 140, 511, 554
HpyCH4IV	ACGT	2 cut(s)	128, 740
HpyF10VI	GCNNNNNNNGC	1 cut(s)	636
HpyF3I	CTNAG	2 cut(s)	188, 195
HpySE526I	ACGT	2 cut(s)	128, 740
Hsp92II	CATG	6 cut(s)	65, 166, 320, 336, 661, 704
HspAI	GCGC	1 cut(s)	531
Kzo9I	GATC	2 cut(s)	18, 367
LmnI	GCTCC	1 cut(s)	295
LpnPI	CCDG	9 cut(s)	101, 128, 357, 429, 500, 556, 665, 693, 749
Lsp1109I	GCAGC	2 cut(s)	123, 171
LweI	GCATC	1 cut(s)	183
MaeI	CTAG	2 cut(s)	69, 381
MaeII	ACGT	2 cut(s)	128, 740
MaeIII	GTNAC	1 cut(s)	511
MalI	GATC	2 cut(s)	20, 369
MboI	GATC	2 cut(s)	18, 367
MboII	GAAGA	2 cut(s)	124, 354
MflI	RGATCY	1 cut(s)	367
MhlI	GDGCHC	1 cut(s)	746
MluCI	AATT	6 cut(s)	82, 249, 411, 500, 602, 706
MlyI	GAGTC	2 cut(s)	366, 574
MmeI	TCCRAC	1 cut(s)	581
MnlI	CCTC	7 cut(s)	104, 154, 157, 197, 382, 463, 537
MseI	TTAA	3 cut(s)	428, 668, 726
MslI	CAYNNNNRTG	2 cut(s)	398, 453
MspCI	CTTAAG	1 cut(s)	725
MspI	CCGG	1 cut(s)	543
MspR9I	CCNGG	1 cut(s)	116
MvaI	CCWGG	1 cut(s)	116
MvnI	CGCG	1 cut(s)	531
MwoI	GCNNNNNNNGC	1 cut(s)	636
NcoI	CCATGG	1 cut(s)	657
NdeII	GATC	2 cut(s)	18, 367
NlaIII	CATG	6 cut(s)	65, 166, 320, 336, 661, 704
NlaIV	GGNNCC	3 cut(s)	291, 342, 485
NspI	RCATGY	1 cut(s)	166
PaeI	GCATGC	1 cut(s)	166
PfeI	GAWTC	1 cut(s)	566
PflMI	CCANNNNNTGG	1 cut(s)	658
PkrI	GCNGC	2 cut(s)	113, 161
PleI	GAGTC	2 cut(s)	365, 574
PpsI	GAGTC	2 cut(s)	365, 574
PshAI	GACNNNNGTC	1 cut(s)	470
Psp6I	CCWGG	1 cut(s)	114
PspGI	CCWGG	1 cut(s)	114
PspN4I	GGNNCC	3 cut(s)	291, 342, 485
PspPI	GGNCC	2 cut(s)	554, 672
PsuI	RGATCY	1 cut(s)	367
RsaI	GTAC	4 cut(s)	103, 200, 223, 650
RsaNI	GTAC	4 cut(s)	102, 199, 222, 649
RseI	CAYNNNNRTG	2 cut(s)	398, 453
Rsr2I	CGGWCCG	1 cut(s)	554
RsrII	CGGWCCG	1 cut(s)	554
SaqAI	TTAA	3 cut(s)	428, 668, 726
SatI	GCNGC	2 cut(s)	112, 160
Sau3AI	GATC	2 cut(s)	18, 367
Sau96I	GGNCC	2 cut(s)	554, 672
ScaI	AGTACT	1 cut(s)	200
SchI	GAGTC	2 cut(s)	366, 574
ScrFI	CCNGG	1 cut(s)	116
SduI	GDGCHC	1 cut(s)	746
SetI	ASST	8 cut(s)	131, 210, 342, 382, 474, 529, 682, 743
SfaNI	GCATC	1 cut(s)	183
SinI	GGWCC	2 cut(s)	554, 672
SmiMI	CAYNNNNRTG	2 cut(s)	398, 453
SmlI	CTYRAG	1 cut(s)	725
SmoI	CTYRAG	1 cut(s)	725
SpeI	ACTAGT	1 cut(s)	68
SphI	GCATGC	1 cut(s)	166
Sse9I	AATT	6 cut(s)	82, 249, 411, 500, 602, 706
SsiI	CCGC	1 cut(s)	746
SspMI	CTAG	2 cut(s)	69, 381
StyD4I	CCNGG	1 cut(s)	114
StyI	CCWWGG	1 cut(s)	657
TaaI	ACNGT	6 cut(s)	38, 51, 101, 140, 511, 554
TaiI	ACGT	2 cut(s)	131, 743
TaqI	TCGA	6 cut(s)	17, 278, 303, 324, 564, 754
TasI	AATT	6 cut(s)	82, 249, 411, 500, 602, 706
TatI	WGTACW	1 cut(s)	198
TfiI	GAWTC	1 cut(s)	566
Tru1I	TTAA	3 cut(s)	428, 668, 726
Tru9I	TTAA	3 cut(s)	428, 668, 726
TscAI	CASTG	2 cut(s)	199, 481
TseI	GCWGC	2 cut(s)	111, 159
TspDTI	ATGAA	3 cut(s)	78, 437, 732
TspGWI	ACGGA	2 cut(s)	482, 496
TspRI	CASTG	2 cut(s)	199, 481
Van91I	CCANNNNNTGG	1 cut(s)	658
Vha464I	CTTAAG	1 cut(s)	725
VpaK11BI	GGWCC	2 cut(s)	554, 672
XapI	RAATTY	2 cut(s)	82, 411
XceI	RCATGY	1 cut(s)	166
XspI	CTAG	2 cut(s)	69, 381
ZrmI	AGTACT	1 cut(s)	200

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.