pycom06g10070

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000675
Physical Location & Seq
Reverse (-)
15420861 .. 15422174
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10070.2

Sequence Viewer

Length: 1095 bp
ATGGCTACCATCGATACCTTCACATTCTTCTTATTCCTCTTCTTCCGTCCTCAAATCACCACCGCCTCGTGTGGTGAACCGAAATGCGCCCCTGAAGGCCCGTCAGTTCGATTCCCTTTCAGCAGGCTAAGTTACAACAACCATTTCTCAAAAAATTGTGGGTATCCAGGGTTTGGTCTCTCATGCAACGACCAACGTCAAACTATTCTCACTCTCCCATTTTCTGAGGACTTCATCGTCCAAAACATCAACTACTTGGATCAAGTTGTAACGATCAACGACCCCGATAACTGCCTCCCTAGACGGTTTTTCCACCGTGACCTCAGTCTTGTAATCGATACACCCTTTGTATACACATATGGCCCTCTAAACTACACGTTCCTCACATGCTCCATCTATAACGAAACCTGGTCTGCACCCTTGATCTCTTGCCTCAGCAGCCAGAAGAACTATGTCATTGTTGTGCCAACGAGTTTTTCATTGCCTCCTGATCAGGAACCCTACCATCGATTTTGCACTGTGATTTCCACAACTTCAGTTCCAAGATCATTTGATTGGCAACATCTTGAACTTGGCGCTCAGTTGACTTGGCATACGCCTGATTGCCGTTTTTGTGAATCAAGTGGTCATCTTTGTGGCTCAAAGAATGGCACAGGTTCCGCAGTTCAGTGCTTTGATTCTTCAAAACCCGGTCTATCAAGGGCTGCAATTTATTGCAGAATGATAAGCGTGGGAATGTCCGTATTGTTAATTGTGGGAATTGCAATTCATGTTCGGGACAAAAAGAGGGCTAGCAATGAGCCCCGCCAACCCAATATGGAATTGTCCATCATAACCGATCAACAACCTTCGGTACTCACAATGGGTCTCGATCGTCCGACCATCGAATTGTATCCCAAGATTCAACTTGGTAAGAGGTCGGAATTTCCAAAACAGCTCACTGACAACACTTGCCCAATATGTTTGGGTGAGTACCGGCCAAAGGAAACCGTGAGGACTATACCACAATGCAACCATTATTTCCATGCTAATTGCATAGATGAATGGCTTAGAAGGAATGCAACATGCCCACTTTGTCGACTTCCGCCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

365

Amino Acids

41.33

Weight (kDa)

8.04

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 173
AccI GTMKAC 2 cut(s) 351, 1078
AciI CCGC 4 cut(s) 63, 660, 805, 1085
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 977
AcsI RAATTY 1 cut(s) 923
AcuI CTGAAG 2 cut(s) 114, 519
AfaI GTAC 2 cut(s) 855, 974
AfiI CCNNNNNNNGG 2 cut(s) 173, 982
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 3 cut(s) 569, 684, 905
AjnI CCWGG 2 cut(s) 166, 407
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 1 cut(s) 937
AluI AGCT 1 cut(s) 937
Alw26I GTCTC 2 cut(s) 182, 872
AlwI GGATC 1 cut(s) 267
AoxI GGCC 3 cut(s) 97, 361, 977
ApeKI GCWGC 2 cut(s) 438, 704
ApoI RAATTY 1 cut(s) 923
AspLEI GCGC 2 cut(s) 89, 578
AspS9I GGNCC 2 cut(s) 98, 362
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 49, 86, 980
AsuNHI GCTAGC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 804
BauI CACGAG 1 cut(s) 67
BbvCI CCTCAGC 1 cut(s) 434
BbvI GCAGC 2 cut(s) 450, 691
BccI CCATC 5 cut(s) 17, 401, 513, 836, 890
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 2 cut(s) 168, 409
BciVI GTATCC 2 cut(s) 174, 903
BclI TGATCA 1 cut(s) 490
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 2 cut(s) 182, 872
BfaI CTAG 2 cut(s) 300, 792
BfoI RGCGCY 1 cut(s) 579
BfuI GTATCC 2 cut(s) 174, 903
BisI GCNGC 2 cut(s) 439, 705
BlsI GCNGC 2 cut(s) 440, 706
Bme1390I CCNGG 3 cut(s) 168, 409, 690
BmgT120I GGNCC 2 cut(s) 98, 362
BmiI GGNNCC 2 cut(s) 498, 658
BmrFI CCNGG 3 cut(s) 168, 409, 690
BmtI GCTAGC 1 cut(s) 795
BoxI GACNNNNGTC 2 cut(s) 195, 324
Bpu10I CCTNAGC 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 690
Bsa29I ATCGAT 3 cut(s) 12, 336, 508
BsaI GGTCTC 2 cut(s) 182, 872
BsaJI CCNNGG 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 982
Bse118I RCCGGY 1 cut(s) 975
Bse3DI GCAATG 2 cut(s) 479, 802
BseBI CCWGG 2 cut(s) 168, 409
BseCI ATCGAT 3 cut(s) 12, 336, 508
BseDI CCNNGG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 173, 982
BseMI GCAATG 2 cut(s) 479, 802
BseMII CTCAG 4 cut(s) 216, 337, 448, 593
BseXI GCAGC 2 cut(s) 450, 691
BsgI GTGCAG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 874
BshFI GGCC 3 cut(s) 99, 363, 979
BshVI ATCGAT 3 cut(s) 12, 336, 508
BsiEI CGRYCG 1 cut(s) 874
BsiSI CCGG 2 cut(s) 690, 976
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 2 cut(s) 173, 982
BsmAI GTCTC 2 cut(s) 182, 872
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 3 cut(s) 99, 363, 979
Bso31I GGTCTC 2 cut(s) 182, 872
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
BspACI CCGC 4 cut(s) 63, 660, 805, 1085
BspANI GGCC 3 cut(s) 99, 363, 979
BspCNI CTCAG 4 cut(s) 217, 336, 447, 592
BspDI ATCGAT 3 cut(s) 12, 336, 508
BspLI GGNNCC 2 cut(s) 498, 658
BspOI GCTAGC 1 cut(s) 795
BspPI GGATC 1 cut(s) 267
BspTNI GGTCTC 2 cut(s) 182, 872
BsrDI GCAATG 2 cut(s) 479, 802
BsrFI RCCGGY 1 cut(s) 975
BssAI RCCGGY 1 cut(s) 975
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
BssNAI GTATAC 1 cut(s) 352
BssSI CACGAG 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 352
Bst2BI CACGAG 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 168, 409
Bst4CI ACNGT 4 cut(s) 306, 317, 520, 991
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 125, 793
BstDEI CTNAG 6 cut(s) 128, 225, 323, 434, 579, 1049
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 2 cut(s) 89, 578
BstKTI GATC 7 cut(s) 262, 276, 426, 493, 548, 841, 874
BstMAI GTCTC 2 cut(s) 182, 872
BstMBI GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
BstMCI CGRYCG 1 cut(s) 874
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstNI CCWGG 2 cut(s) 168, 409
BstNSI RCATGY 2 cut(s) 390, 1068
BstPAI GACNNNNGTC 2 cut(s) 195, 324
BstSCI CCNGG 3 cut(s) 166, 407, 688
BstV1I GCAGC 2 cut(s) 450, 691
BstZ17I GTATAC 1 cut(s) 352
Bsu15I ATCGAT 3 cut(s) 12, 336, 508
BsuI GTATCC 2 cut(s) 174, 903
BsuRI GGCC 3 cut(s) 99, 363, 979
BsuTUI ATCGAT 3 cut(s) 12, 336, 508
BtsIMutI CAGTG 3 cut(s) 516, 674, 939
Cac8I GCNNGC 2 cut(s) 125, 793
CfoI GCGC 2 cut(s) 89, 578
Cfr10I RCCGGY 1 cut(s) 975
Cfr13I GGNCC 2 cut(s) 98, 362
ClaI ATCGAT 3 cut(s) 12, 336, 508
CsiI ACCWGGT 1 cut(s) 407
Csp6I GTAC 2 cut(s) 854, 973
CviAII CATG 5 cut(s) 183, 387, 770, 1025, 1065
CviQI GTAC 2 cut(s) 854, 973
DdeI CTNAG 6 cut(s) 128, 225, 323, 434, 579, 1049
DpnI GATC 7 cut(s) 261, 275, 425, 492, 547, 840, 873
DpnII GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 977
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 2 cut(s) 182, 872
Eco57I CTGAAG 2 cut(s) 114, 519
EcoRII CCWGG 2 cut(s) 166, 407
EcoT38I GRGCYC 1 cut(s) 804
FaeI CATG 5 cut(s) 186, 390, 773, 1028, 1068
FaqI GGGAC 1 cut(s) 791
FatI CATG 5 cut(s) 182, 386, 769, 1024, 1064
FauI CCCGC 1 cut(s) 812
FauNDI CATATG 1 cut(s) 358
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 2 cut(s) 351, 1078
Fnu4HI GCNGC 2 cut(s) 439, 705
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 2 cut(s) 439, 705
FspBI CTAG 2 cut(s) 300, 792
GlaI GCGC 2 cut(s) 88, 577
GluI GCNGC 2 cut(s) 439, 705
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 3 cut(s) 99, 363, 979
HapII CCGG 2 cut(s) 690, 976
HhaI GCGC 2 cut(s) 89, 578
Hin1II CATG 5 cut(s) 186, 390, 773, 1028, 1068
Hin6I GCGC 2 cut(s) 87, 576
HinP1I GCGC 2 cut(s) 87, 576
HincII GTYRAC 2 cut(s) 585, 1079
HindII GTYRAC 2 cut(s) 585, 1079
HinfI GANTC 4 cut(s) 111, 617, 677, 901
HpaII CCGG 2 cut(s) 690, 976
HphI GGTGA 3 cut(s) 49, 86, 980
Hpy166II GTNNAC 4 cut(s) 77, 352, 585, 1079
Hpy188I TCNGA 3 cut(s) 226, 879, 922
Hpy188III TCNNGA 5 cut(s) 488, 494, 566, 776, 869
Hpy8I GTNNAC 4 cut(s) 77, 352, 585, 1079
HpyAV CCTTC 4 cut(s) 28, 89, 858, 1047
HpyCH4III ACNGT 4 cut(s) 306, 317, 520, 991
HpyCH4IV ACGT 2 cut(s) 196, 377
HpyCH4V TGCA 9 cut(s) 186, 416, 516, 707, 717, 764, 1011, 1035, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 6 cut(s) 128, 225, 323, 434, 579, 1049
HpySE526I ACGT 2 cut(s) 196, 377
Hsp92II CATG 5 cut(s) 186, 390, 773, 1028, 1068
HspAI GCGC 2 cut(s) 87, 576
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 2 cut(s) 450, 691
MabI ACCWGGT 1 cut(s) 407
MaeI CTAG 2 cut(s) 300, 792
MaeII ACGT 2 cut(s) 196, 377
MaeIII GTNAC 3 cut(s) 131, 268, 317
MalI GATC 7 cut(s) 261, 275, 425, 492, 547, 840, 873
MboI GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
MboII GAAGA 5 cut(s) 19, 31, 34, 457, 672
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 9 cut(s) 154, 708, 750, 759, 765, 821, 887, 923, 1030
MmeI TCCRAC 2 cut(s) 900, 902
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 2 cut(s) 461, 633
MspI CCGG 2 cut(s) 690, 976
MspR9I CCNGG 3 cut(s) 168, 409, 690
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 2 cut(s) 168, 409
MwoI GCNNNNNNNGC 1 cut(s) 438
NciI CCSGG 1 cut(s) 690
NdeI CATATG 1 cut(s) 358
NdeII GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
NheI GCTAGC 1 cut(s) 791
NlaIII CATG 5 cut(s) 186, 390, 773, 1028, 1068
NlaIV GGNNCC 2 cut(s) 498, 658
NmuCI GTSAC 1 cut(s) 317
NspI RCATGY 2 cut(s) 390, 1068
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 4 cut(s) 111, 617, 677, 901
PflMI CCANNNNNTGG 1 cut(s) 173
PkrI GCNGC 2 cut(s) 440, 706
Ple19I CGATCG 1 cut(s) 874
PshAI GACNNNNGTC 2 cut(s) 195, 324
Psp6I CCWGG 2 cut(s) 166, 407
PspGI CCWGG 2 cut(s) 166, 407
PspN4I GGNNCC 2 cut(s) 498, 658
PspPI GGNCC 2 cut(s) 98, 362
PvuI CGATCG 1 cut(s) 874
RsaI GTAC 2 cut(s) 855, 974
RsaNI GTAC 2 cut(s) 854, 973
RseI CAYNNNNRTG 2 cut(s) 461, 633
SalI GTCGAC 1 cut(s) 1077
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 2 cut(s) 439, 705
Sau3AI GATC 7 cut(s) 259, 273, 423, 490, 545, 838, 871
Sau96I GGNCC 2 cut(s) 98, 362
ScrFI CCNGG 3 cut(s) 168, 409, 690
SduI GDGCHC 1 cut(s) 804
SetI ASST 9 cut(s) 20, 199, 324, 380, 410, 658, 850, 920, 939
SexAI ACCWGGT 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 461, 633
Sse9I AATT 9 cut(s) 154, 708, 750, 759, 765, 821, 887, 923, 1030
SsiI CCGC 4 cut(s) 63, 660, 805, 1085
SspMI CTAG 2 cut(s) 300, 792
StyD4I CCNGG 3 cut(s) 166, 407, 688
TaaI ACNGT 4 cut(s) 306, 317, 520, 991
TaiI ACGT 2 cut(s) 199, 380
TaqI TCGA 7 cut(s) 12, 109, 336, 508, 870, 885, 1078
TasI AATT 9 cut(s) 154, 708, 750, 759, 765, 821, 887, 923, 1030
TfiI GAWTC 4 cut(s) 111, 617, 677, 901
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 3 cut(s) 523, 674, 946
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 2 cut(s) 438, 704
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 4 cut(s) 223, 468, 758, 1056
TspGWI ACGGA 2 cut(s) 35, 730
TspRI CASTG 3 cut(s) 523, 674, 946
Van91I CCANNNNNTGG 1 cut(s) 173
XapI RAATTY 1 cut(s) 923
XceI RCATGY 2 cut(s) 390, 1068
XmiI GTMKAC 2 cut(s) 351, 1078
XspI CTAG 2 cut(s) 300, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.