Prupe.5G119600_v2.0.a1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000675
Physical Location & Seq
Reverse (-)
11993889 .. 11995296
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G119600.1

Sequence Viewer

Length: 1167 bp
ATGGAGAACATAAAAATCTTCACCTTCTTCATCTTATTCTTGTTCCTCCTCTTCCTTCCTCACACCGTTACCAAAACCACCTGCGGTAGCTCGACATGCACTGCCGGCGGCCCACTGGTTAAGTTCCCTTTCCAACTAAGAAACTACCAAGCTTCAAGTTGTGGGTATCCAGGGTTTGATGTCTCATGTCACAACAACAGTCAAACCATTCTCACTCTCCCATCTTTTGGGGACTTCACCGTTGAAAGCATCGACTACAATGGCCAAGTCGTAACGATCAACGATCCTGATAAATGCCTCCCTGCACGGCTCCTCAACCATGAAACCAGTCTTGTAGAATATTCACCCTTTTCATACTTAAGCTTTGGCGGCCCTATAAACTACACGTTCCTCAACTGCTCCTCCTCTAAACCATCCATCTTGCCTGCACGCATCATCTCTTGCCTCAGCACTGGCTACTACAAGGTCATTGCTTTGCCTACGAGTTGGCTTTTGCCACCGGATCCGATATTATTGCCTCATTGCAATGCAATTTCGACAATTTTGGTTCCAACTTCATTAGAGTGGGAGCATTTTAACGATGGTCTCGAGTTGACATGGGAGGTGCCTGATTGTCGTTCTTGTGAAGTAAGGGGTCAGGCTTGTGGGTTAAAAAATGGAAAAAGCTTGGAAATTCGATGCTTTGGTCCTTCCAAGATACACTCGGTTAGAGCCTCTTCCGGTCCCTCAAGAGCTGCAAAGTACGGCATAATGATGGCCATCGGAATTCCTCCGGTATTGTTCATCATTTGGCTTGTACTCTATATGTGCGGCAGAATGATGATCGAAGTTAATGGTCAAAACCACCGGCATATCACAGAATTATCCATCGTAACCAATCAGCAACTTCCCACAATCGTAACTGGCCTTGATGCTCCCACCATCGAATCATATTCCACGACTCTGCTAGGCGAGAGTTGGGAACTGCCCAAGCCTAATGACAACACTTGCCCAATATGTTTGTCTGAGTACCAATCCAAGGAAACACTGCGGACAATACCAAAATGCAACCATTATTTCCATGCTAATTGTGTAGACAAGTGGCTTCGATTGAATGCAACATGCCCGCTTTGTCGAAATCCACAGGATAGAGATAACAATATAAGTCATTTAGTGATTGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

389

Amino Acids

43.27

Weight (kDa)

6.41

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 604
AccB7I CCANNNNNTGG 1 cut(s) 227
AccI GTMKAC 1 cut(s) 1074
AciI CCGC 6 cut(s) 84, 108, 369, 810, 1030, 1106
AclWI GGATC 3 cut(s) 278, 497, 510
AcoI YGGCCR 2 cut(s) 262, 756
AcsI RAATTY 2 cut(s) 672, 765
AfaI GTAC 3 cut(s) 743, 798, 1010
AfiI CCNNNNNNNGG 2 cut(s) 227, 1018
AflII CTTAAG 1 cut(s) 358
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 3 cut(s) 156, 245, 1093
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 5 cut(s) 90, 152, 363, 666, 734
AluI AGCT 5 cut(s) 90, 152, 363, 666, 734
Alw26I GTCTC 2 cut(s) 187, 590
AlwI GGATC 3 cut(s) 278, 497, 510
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 5 cut(s) 109, 262, 370, 756, 904
ApeKI GCWGC 1 cut(s) 734
ApoI RAATTY 2 cut(s) 672, 765
AspS9I GGNCC 4 cut(s) 110, 371, 686, 722
AsuHPI GGTGA 3 cut(s) 13, 229, 336
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 2 cut(s) 686, 722
BalI TGGCCA 2 cut(s) 264, 758
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 604
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 721
BccI CCATC 8 cut(s) 229, 421, 425, 575, 748, 767, 875, 929
BceAI ACGGC 2 cut(s) 323, 760
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 1 cut(s) 171
BciVI GTATCC 1 cut(s) 177
BcoDI GTCTC 2 cut(s) 187, 590
BfaI CTAG 1 cut(s) 947
BfrI CTTAAG 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 177
BisI GCNGC 4 cut(s) 109, 370, 735, 811
BlsI GCNGC 4 cut(s) 110, 371, 736, 812
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 2 cut(s) 686, 722
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 4 cut(s) 110, 371, 686, 722
BmiI GGNNCC 5 cut(s) 311, 504, 549, 606, 724
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 4 cut(s) 258, 441, 668, 901
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 712
BsaBI GATNNNNATC 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 2 cut(s) 170, 1017
BsaWI WCCGGW 3 cut(s) 499, 719, 772
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 1018
Bse118I RCCGGY 2 cut(s) 104, 846
Bse1I ACTGG 4 cut(s) 120, 327, 457, 907
Bse3DI GCAATG 3 cut(s) 468, 520, 532
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 170, 1017
BseGI GGATG 1 cut(s) 413
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 2 cut(s) 227, 1018
BseMI GCAATG 3 cut(s) 468, 520, 532
BseMII CTCAG 2 cut(s) 460, 996
BseNI ACTGG 4 cut(s) 120, 327, 457, 907
BseRI GAGGAG 4 cut(s) 38, 302, 391, 394
BseXI GCAGC 1 cut(s) 721
BsgI GTGCAG 2 cut(s) 288, 411
BshFI GGCC 5 cut(s) 111, 264, 372, 758, 906
BshNI GGYRCC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 5 cut(s) 105, 500, 720, 773, 847
BslFI GGGAC 2 cut(s) 245, 708
BslI CCNNNNNNNGG 2 cut(s) 227, 1018
BsmAI GTCTC 2 cut(s) 187, 590
BsmFI GGGAC 2 cut(s) 245, 708
BsmI GAATGC 1 cut(s) 1099
BsnI GGCC 5 cut(s) 111, 264, 372, 758, 906
Bso31I GGTCTC 1 cut(s) 590
BsoBI CYCGRG 1 cut(s) 587
Bsp143I GATC 4 cut(s) 276, 283, 502, 822
BspACI CCGC 6 cut(s) 84, 108, 369, 810, 1030, 1106
BspANI GGCC 5 cut(s) 111, 264, 372, 758, 906
BspCNI CTCAG 2 cut(s) 459, 997
BspLI GGNNCC 5 cut(s) 311, 504, 549, 606, 724
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 278, 497, 510
BspT107I GGYRCC 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 590
BsrDI GCAATG 3 cut(s) 468, 520, 532
BsrFI RCCGGY 2 cut(s) 104, 846
BsrI ACTGG 4 cut(s) 120, 327, 457, 907
BssAI RCCGGY 2 cut(s) 104, 846
BssECI CCNNGG 2 cut(s) 170, 1017
BssMI GATC 4 cut(s) 276, 283, 502, 822
BssT1I CCWWGG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 67, 200, 241
Bst6I CTCTTC 2 cut(s) 56, 721
BstAFI CTTAAG 1 cut(s) 358
BstC8I GCNNGC 4 cut(s) 106, 426, 430, 1106
BstDEI CTNAG 3 cut(s) 137, 446, 1005
BstF5I GGATG 1 cut(s) 413
BstKTI GATC 4 cut(s) 279, 286, 505, 825
BstMAI GTCTC 2 cut(s) 187, 590
BstMBI GATC 4 cut(s) 276, 283, 502, 822
BstMWI GCNNNNNNNGC 3 cut(s) 96, 105, 369
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 2 cut(s) 99, 1104
BstSCI CCNGG 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 721
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuI GTATCC 1 cut(s) 177
BsuRI GGCC 5 cut(s) 111, 264, 372, 758, 906
BtsCI GGATG 1 cut(s) 413
BtsI GCAGTG 2 cut(s) 99, 1025
BtsIMutI CAGTG 4 cut(s) 99, 113, 450, 1025
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 4 cut(s) 106, 426, 430, 1106
Cfr10I RCCGGY 2 cut(s) 104, 846
Cfr13I GGNCC 4 cut(s) 110, 371, 686, 722
Csp6I GTAC 3 cut(s) 742, 797, 1009
CviAII CATG 6 cut(s) 96, 186, 320, 597, 1061, 1101
CviQI GTAC 3 cut(s) 742, 797, 1009
DdeI CTNAG 3 cut(s) 137, 446, 1005
DpnI GATC 4 cut(s) 278, 285, 504, 824
DpnII GATC 4 cut(s) 276, 283, 502, 822
EaeI YGGCCR 2 cut(s) 262, 756
Eam1104I CTCTTC 2 cut(s) 56, 721
EarI CTCTTC 2 cut(s) 56, 721
Eco130I CCWWGG 1 cut(s) 1017
Eco31I GGTCTC 1 cut(s) 590
Eco47I GGWCC 2 cut(s) 686, 722
Eco88I CYCGRG 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 1017
FaeI CATG 6 cut(s) 99, 189, 323, 600, 1064, 1104
FaqI GGGAC 2 cut(s) 245, 708
FatI CATG 6 cut(s) 95, 185, 319, 596, 1060, 1100
FauI CCCGC 1 cut(s) 1113
FblI GTMKAC 1 cut(s) 1074
Fnu4HI GCNGC 4 cut(s) 109, 370, 735, 811
FokI GGATG 1 cut(s) 400
Fsp4HI GCNGC 4 cut(s) 109, 370, 735, 811
FspBI CTAG 1 cut(s) 947
GluI GCNGC 4 cut(s) 109, 370, 735, 811
HaeIII GGCC 5 cut(s) 111, 264, 372, 758, 906
HapII CCGG 5 cut(s) 105, 500, 720, 773, 847
Hin1II CATG 6 cut(s) 99, 189, 323, 600, 1064, 1104
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 3 cut(s) 150, 361, 664
HinfI GANTC 2 cut(s) 926, 940
HpaII CCGG 5 cut(s) 105, 500, 720, 773, 847
HphI GGTGA 3 cut(s) 13, 229, 336
Hpy166II GTNNAC 2 cut(s) 594, 1075
Hpy188I TCNGA 3 cut(s) 507, 764, 1006
Hpy188III TCNNGA 3 cut(s) 287, 587, 729
Hpy8I GTNNAC 2 cut(s) 594, 1075
HpyAV CCTTC 3 cut(s) 34, 65, 699
HpyCH4III ACNGT 3 cut(s) 67, 200, 241
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 8 cut(s) 99, 305, 428, 525, 530, 737, 1047, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 105, 369
HpyF3I CTNAG 3 cut(s) 137, 446, 1005
HpySE526I ACGT 1 cut(s) 386
Hsp92II CATG 6 cut(s) 99, 189, 323, 600, 1064, 1104
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 276, 283, 502, 822
LmnI GCTCC 4 cut(s) 315, 404, 568, 919
Lsp1109I GCAGC 1 cut(s) 721
LweI GCATC 4 cut(s) 258, 441, 668, 901
MaeI CTAG 1 cut(s) 947
MaeII ACGT 1 cut(s) 386
MaeIII GTNAC 5 cut(s) 67, 188, 271, 871, 898
MalI GATC 4 cut(s) 278, 285, 504, 824
MboI GATC 4 cut(s) 276, 283, 502, 822
MboII GAAGA 4 cut(s) 10, 19, 43, 708
MflI RGATCY 1 cut(s) 502
MlsI TGGCCA 2 cut(s) 264, 758
MluCI AATT 6 cut(s) 531, 540, 672, 765, 860, 1066
MluNI TGGCCA 2 cut(s) 264, 758
MlyI GAGTC 1 cut(s) 934
MmeI TCCRAC 2 cut(s) 157, 575
Mox20I TGGCCA 2 cut(s) 264, 758
MroNI GCCGGC 1 cut(s) 104
MscI TGGCCA 2 cut(s) 264, 758
MseI TTAA 5 cut(s) 120, 359, 576, 650, 831
MslI CAYNNNNRTG 3 cut(s) 525, 562, 752
Msp20I TGGCCA 2 cut(s) 264, 758
MspCI CTTAAG 1 cut(s) 358
MspI CCGG 5 cut(s) 105, 500, 720, 773, 847
MspR9I CCNGG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 1099
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 96, 105, 369
NaeI GCCGGC 1 cut(s) 106
NdeII GATC 4 cut(s) 276, 283, 502, 822
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 6 cut(s) 99, 189, 323, 600, 1064, 1104
NlaIV GGNNCC 5 cut(s) 311, 504, 549, 606, 724
NmuCI GTSAC 1 cut(s) 188
NspI RCATGY 2 cut(s) 99, 1104
PaeR7I CTCGAG 1 cut(s) 587
PaqCI CACCTGC 1 cut(s) 89
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PctI GAATGC 1 cut(s) 1099
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 926
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 4 cut(s) 110, 371, 736, 812
PleI GAGTC 1 cut(s) 934
PpsI GAGTC 1 cut(s) 934
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 5 cut(s) 311, 504, 549, 606, 724
PspPI GGNCC 4 cut(s) 110, 371, 686, 722
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 3 cut(s) 743, 798, 1010
RsaNI GTAC 3 cut(s) 742, 797, 1009
RseI CAYNNNNRTG 3 cut(s) 525, 562, 752
SaqAI TTAA 5 cut(s) 120, 359, 576, 650, 831
SatI GCNGC 4 cut(s) 109, 370, 735, 811
Sau3AI GATC 4 cut(s) 276, 283, 502, 822
Sau96I GGNCC 4 cut(s) 110, 371, 686, 722
SchI GAGTC 1 cut(s) 934
ScrFI CCNGG 1 cut(s) 171
SfaNI GCATC 4 cut(s) 258, 441, 668, 901
Sfr274I CTCGAG 1 cut(s) 587
SinI GGWCC 2 cut(s) 686, 722
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 525, 562, 752
SmlI CTYRAG 3 cut(s) 358, 587, 727
SmoI CTYRAG 3 cut(s) 358, 587, 727
Sse9I AATT 6 cut(s) 531, 540, 672, 765, 860, 1066
SsiI CCGC 6 cut(s) 84, 108, 369, 810, 1030, 1106
SspI AATATT 1 cut(s) 341
SspMI CTAG 1 cut(s) 947
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 3 cut(s) 67, 200, 241
TaiI ACGT 1 cut(s) 389
TaqI TCGA 9 cut(s) 92, 252, 536, 588, 676, 825, 924, 1087, 1114
TasI AATT 6 cut(s) 531, 540, 672, 765, 860, 1066
TatI WGTACW 1 cut(s) 796
TauI GCSGC 3 cut(s) 111, 372, 813
TfiI GAWTC 1 cut(s) 926
Tru1I TTAA 5 cut(s) 120, 359, 576, 650, 831
Tru9I TTAA 5 cut(s) 120, 359, 576, 650, 831
TscAI CASTG 4 cut(s) 106, 120, 457, 1032
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 1 cut(s) 734
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 5 cut(s) 19, 336, 342, 546, 772
TspRI CASTG 4 cut(s) 106, 120, 457, 1032
Van91I CCANNNNNTGG 1 cut(s) 227
Vha464I CTTAAG 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 686, 722
XapI RAATTY 2 cut(s) 672, 765
XceI RCATGY 2 cut(s) 99, 1104
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 1074
XspI CTAG 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.