Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0000675

Physical Location & Seq

Genomic Coordinates Reverse (-)

2690601 .. 2691812

Gene Structure 1212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g04554.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1113 bp

ATGGCACTCTTGGAACTCTTTAGGTTCTTCTTCTTCTTGCTTCCTCACATAGCATCCGCCTCCTGCGGCACATCAAAATGCACCCACCAAGGGGTAAAGGTTAAATTCCCTTTCTGGCTGACAAACCGCCAAGACTCCCGTTGTGGGTATCCAGGGTTTGGTCTCACATGCGATAGTGAAAGGAAGCAAACCCTTGTCACTCTCCCGTCTACATCAGGGGACTTCTTCGTCAACAGCATCGACTACATGGACCAAGTCGTATCGATCATCGACACCACCGCGAAAGGCTGCCTCATCAAGCGGTTCATGGACCAGGAAATCAGCCTCAATGATTCCGCCTTCTTATATGCTAATGGTGGACAGTACAAGTTCTTCAATTGCTCGCCTAAAGCAGTGGAAGTACTAGCTTATTATCCAACAATCTCTTGCCTCAGCCGTGAGGACAGCAAGGTCATTCCTGTGCCATCCAGTTCAATTTTATTGCCGTCGTCTGCGCCACCAATACCATCTGATCTTTGCTCTCTGATGTCGAAAGCTTTTGTTCCGGTGGCCTACATACCATTTGTTCCACCAAATGCATCTGATTGGGAAAATATTAACTTTGGTTTTCGGTTGGCATGGTCAGTGCCTGATTGTCGTTACTGTGAAATAGTTCATAGAGCTTGTCGATTTGAGAGTCCTACAAATATGGAGATTGGCTGTTCTGCTCCAAAAGGTCTCTCAAGTGCTGCAAGAACTGGCCTAATTATAGGCCTGGGAATCCCAGGGTTTGTGGGAATCTCTGCGCTTGTAATGTTTATAATGGAAAGGGTGAGGATCTGTCGTCAGGGCAACCACACAACGGCGGAACTATCCACGGTAACAAATGAACAACCTCCAGTAGTCACAGTGGTCGGCCTTGATGCTGAAACCATCGAATCGTATCCCCAGACTCAACTTGGTGAGAGCGTCGATTTACCAAACCCAAGTGATAAAACTTGCTCAATATGTTTGGCCGAGTACCTGGAAAAGGAAACTCTCAGGACTATACCTGAATGCAACCATTATTTTCATGCTACATGTATAGACGAATGGCTTAGAAAGAATGCAACGTGTCCCCTTTGTCGAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind WAK_assoc zf-RING_2

Protein Analysis

371

Amino Acids

40.95

Weight (kDa)

5.75

Isoelectric Point (pI)

50.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	22 - 91	1.4e-15	Wall-associated receptor kinase galacturonan-binding
zf-rbx1	PF12678	323 - 369	9.3e-11	RING-H2 zinc finger domain
zf-RING_2	PF13639	326 - 369	2.8e-14	Ring finger domain
zf-RING_11	PF17123	327 - 355	1.8e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	327 - 368	2.9e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	327 - 368	9.6e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	327 - 369	6.6e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000675)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g04540 FvH4_5g04550 FvH4_5g04551 FvH4_5g04552 FvH4_5g04554 FvH4_5g29040
malus_domestica	MD06G1106600.v1.1 MD14G1128600.v1.1 MD14G1128700.v1.1
prunus_persica	Prupe.5G118700_v2.0.a1 Prupe.5G118800_v2.0.a1 Prupe.5G119000_v2.0.a1 Prupe.5G119300_v2.0.a1 Prupe.5G119500_v2.0.a1 Prupe.5G119600_v2.0.a1 Prupe.5G119700_v2.0.a1 Prupe.5G119800_v2.0.a1 Prupe.5G119900_v2.0.a1
pyrus_communis	pycom06g10070 pycom06g10090
rosa_chinensis	RchiOBHm_Chr7g0194821 RchiOBHm_Chr7g0194841 RchiOBHm_Chr7g0194851 RchiOBHm_Chr7g0194861 RchiOBHm_Chr7g0194871 RchiOBHm_Chr7g0226371
rosa_laevigata	RLG00000001745 RLG00000004159 RLG00000004160 RLG00000004162 RLG00000004163 RLG00000004166 RLG00000013439
rosa_multiflora	Rmu_co8140360.1_g000001 Rmu_co8197174.1_g000001 Rmu_co8260763.1_g000001 Rmu_co8287607.1_g000001 Rmu_co8453499.1_g000001 Rmu_sc0011834.1_g000004 Rmu_sc0017428.1_g000003 Rmu_sc0040407.1_g000001
rosa_roxburghii	Rroxscaffold_3G00260910 Rroxscaffold_3G00260920
rosa_rugosa	Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0018600 Rorug07G0236700
rosa_samantha	Rh7AG146400 Rh7AG146500 Rh7AG146600 Rh7AG383700 Rh7BG146300 Rh7BG146400 Rh7BG146500 Rh7BG146600 Rh7BG146900 Rh7BG147100 Rh7BG147200 Rh7BG147300 Rh7BG370800 Rh7CG150400 Rh7CG150500 Rh7CG150600 Rh7CG403300 Rh7DG147600 Rh7DG147700 Rh7DG147800 Rh7DG382100 Rh7DG482500
rosa_wichuraiana	Rw7G012490 Rw7G012500 Rw7G012510 Rw7G032160 Rw7G032220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	800
AasI	GACNNNNNNGTC	2 cut(s)	227, 449
AccB7I	CCANNNNNTGG	1 cut(s)	158
AccI	GTMKAC	1 cut(s)	209
AccII	CGCG	1 cut(s)	281
AciI	CCGC	7 cut(s)	57, 66, 127, 279, 301, 336, 845
AclWI	GGATC	1 cut(s)	824
AcoI	YGGCCR	1 cut(s)	993
AcsI	RAATTY	1 cut(s)	104
AfaI	GTAC	3 cut(s)	365, 402, 1001
AfiI	CCNNNNNNNGG	6 cut(s)	90, 91, 144, 158, 841, 1009
AflIII	ACRYGT	2 cut(s)	1058, 1091
AgsI	TTSAA	2 cut(s)	376, 474
AjnI	CCWGG	5 cut(s)	151, 312, 753, 763, 1002
AluBI	AGCT	3 cut(s)	407, 536, 662
AluI	AGCT	3 cut(s)	407, 536, 662
Alw26I	GTCTC	2 cut(s)	167, 722
AlwI	GGATC	1 cut(s)	824
AlwNI	CAGNNNCTG	1 cut(s)	629
AoxI	GGCC	5 cut(s)	549, 739, 751, 895, 993
ApeKI	GCWGC	2 cut(s)	288, 728
ApoI	RAATTY	1 cut(s)	104
Asp700I	GAANNNNTTC	1 cut(s)	651
AspLEI	GCGC	2 cut(s)	496, 787
AspS9I	GGNCC	2 cut(s)	250, 310
AsuHPI	GGTGA	2 cut(s)	823, 953
AvaII	GGWCC	2 cut(s)	250, 310
BarI	GAAGNNNNNNTAC	2 cut(s)	356, 388
BbvCI	CCTCAGC	1 cut(s)	431
BbvI	GCAGC	2 cut(s)	275, 715
BccI	CCATC	3 cut(s)	472, 514, 920
BceAI	ACGGC	3 cut(s)	420, 469, 858
BciT130I	CCWGG	5 cut(s)	153, 314, 755, 765, 1004
BciVI	GTATCC	2 cut(s)	159, 933
BcoDI	GTCTC	2 cut(s)	167, 722
BfaI	CTAG	1 cut(s)	404
BfuI	GTATCC	2 cut(s)	159, 933
BisI	GCNGC	3 cut(s)	67, 289, 729
BlsI	GCNGC	3 cut(s)	68, 290, 730
BmcAI	AGTACT	1 cut(s)	402
Bme1390I	CCNGG	5 cut(s)	153, 314, 755, 765, 1004
Bme18I	GGWCC	2 cut(s)	250, 310
BmgT120I	GGNCC	2 cut(s)	250, 310
BmrFI	CCNGG	5 cut(s)	153, 314, 755, 765, 1004
BmsI	GCATC	4 cut(s)	62, 246, 587, 892
BpmI	CTGGAG	1 cut(s)	861
Bpu10I	CCTNAGC	1 cut(s)	431
BpuEI	CTTGAG	1 cut(s)	706
Bsa29I	ATCGAT	1 cut(s)	263
BsaI	GGTCTC	2 cut(s)	167, 722
BsaJI	CCNNGG	6 cut(s)	88, 152, 754, 763, 764, 855
BsaWI	WCCGGW	1 cut(s)	544
Bsc4I	CCNNNNNNNGG	6 cut(s)	90, 91, 144, 158, 841, 1009
Bse1I	ACTGG	3 cut(s)	468, 742, 878
BseBI	CCWGG	5 cut(s)	153, 314, 755, 765, 1004
BseCI	ATCGAT	1 cut(s)	263
BseDI	CCNNGG	6 cut(s)	88, 152, 754, 763, 764, 855
BseGI	GGATG	2 cut(s)	53, 464
BseLI	CCNNNNNNNGG	6 cut(s)	90, 91, 144, 158, 841, 1009
BseMII	CTCAG	2 cut(s)	445, 1033
BseNI	ACTGG	3 cut(s)	468, 742, 878
BseXI	GCAGC	2 cut(s)	275, 715
Bsh1236I	CGCG	1 cut(s)	281
BshFI	GGCC	5 cut(s)	551, 741, 753, 897, 995
BshVI	ATCGAT	1 cut(s)	263
BsiSI	CCGG	1 cut(s)	545
BslFI	GGGAC	2 cut(s)	233, 1080
BslI	CCNNNNNNNGG	6 cut(s)	90, 91, 144, 158, 841, 1009
BsmAI	GTCTC	2 cut(s)	167, 722
BsmFI	GGGAC	2 cut(s)	233, 1080
BsmI	GAATGC	2 cut(s)	1040, 1090
BsnI	GGCC	5 cut(s)	551, 741, 753, 897, 995
Bso31I	GGTCTC	2 cut(s)	167, 722
Bsp143I	GATC	3 cut(s)	264, 511, 816
BspACI	CCGC	7 cut(s)	57, 66, 127, 279, 301, 336, 845
BspANI	GGCC	5 cut(s)	551, 741, 753, 897, 995
BspCNI	CTCAG	2 cut(s)	444, 1032
BspDI	ATCGAT	1 cut(s)	263
BspFNI	CGCG	1 cut(s)	281
BspPI	GGATC	1 cut(s)	824
BspTNI	GGTCTC	2 cut(s)	167, 722
BsrI	ACTGG	3 cut(s)	468, 742, 878
BssECI	CCNNGG	6 cut(s)	88, 152, 754, 763, 764, 855
BssMI	GATC	3 cut(s)	264, 511, 816
BssT1I	CCWWGG	1 cut(s)	88
Bst2UI	CCWGG	5 cut(s)	153, 314, 755, 765, 1004
Bst4CI	ACNGT	4 cut(s)	363, 644, 859, 889
BstC8I	GCNNGC	1 cut(s)	383
BstDEI	CTNAG	3 cut(s)	431, 1019, 1076
BstDSI	CCRYGG	1 cut(s)	855
BstENI	CCTNNNNNAGG	1 cut(s)	1007
BstF5I	GGATG	2 cut(s)	53, 464
BstFNI	CGCG	1 cut(s)	281
BstHHI	GCGC	2 cut(s)	496, 787
BstKTI	GATC	3 cut(s)	267, 514, 819
BstMAI	GTCTC	2 cut(s)	167, 722
BstMBI	GATC	3 cut(s)	264, 511, 816
BstNI	CCWGG	5 cut(s)	153, 314, 755, 765, 1004
BstNSI	RCATGY	2 cut(s)	171, 1062
BstSCI	CCNGG	5 cut(s)	151, 312, 753, 763, 1002
BstUI	CGCG	1 cut(s)	281
BstV1I	GCAGC	2 cut(s)	275, 715
BstX2I	RGATCY	1 cut(s)	816
BstYI	RGATCY	1 cut(s)	816
Bsu15I	ATCGAT	1 cut(s)	263
BsuI	GTATCC	2 cut(s)	159, 933
BsuRI	GGCC	5 cut(s)	551, 741, 753, 897, 995
BsuTUI	ATCGAT	1 cut(s)	263
BtgI	CCRYGG	1 cut(s)	855
BtsCI	GGATG	2 cut(s)	53, 464
BtsI	GCAGTG	1 cut(s)	399
BtsIMutI	CAGTG	3 cut(s)	399, 630, 894
Cac8I	GCNNGC	1 cut(s)	383
CaiI	CAGNNNCTG	1 cut(s)	629
CfoI	GCGC	2 cut(s)	496, 787
Cfr13I	GGNCC	2 cut(s)	250, 310
ClaI	ATCGAT	1 cut(s)	263
CseI	GACGC	1 cut(s)	937
Csp6I	GTAC	3 cut(s)	364, 401, 1000
CviAII	CATG	6 cut(s)	168, 247, 307, 618, 1052, 1059
CviQI	GTAC	3 cut(s)	364, 401, 1000
DdeI	CTNAG	3 cut(s)	431, 1019, 1076
DpnI	GATC	3 cut(s)	266, 513, 818
DpnII	GATC	3 cut(s)	264, 511, 816
DrdI	GACNNNNNNGTC	2 cut(s)	227, 449
DseDI	GACNNNNNNGTC	2 cut(s)	227, 449
EaeI	YGGCCR	1 cut(s)	993
EciI	GGCGGA	3 cut(s)	46, 325, 860
Eco130I	CCWWGG	1 cut(s)	88
Eco147I	AGGCCT	1 cut(s)	753
Eco31I	GGTCTC	2 cut(s)	167, 722
Eco47I	GGWCC	2 cut(s)	250, 310
EcoNI	CCTNNNNNAGG	1 cut(s)	1007
EcoRII	CCWGG	5 cut(s)	151, 312, 753, 763, 1002
EcoT14I	CCWWGG	1 cut(s)	88
EcoT22I	ATGCAT	1 cut(s)	580
ErhI	CCWWGG	1 cut(s)	88
FaeI	CATG	6 cut(s)	171, 250, 310, 621, 1055, 1062
FaqI	GGGAC	2 cut(s)	233, 1080
FatI	CATG	6 cut(s)	167, 246, 306, 617, 1051, 1058
FblI	GTMKAC	1 cut(s)	209
Fnu4HI	GCNGC	3 cut(s)	67, 289, 729
FokI	GGATG	2 cut(s)	40, 451
Fsp4HI	GCNGC	3 cut(s)	67, 289, 729
FspBI	CTAG	1 cut(s)	404
GlaI	GCGC	2 cut(s)	495, 786
GluI	GCNGC	3 cut(s)	67, 289, 729
GsuI	CTGGAG	1 cut(s)	861
HaeIII	GGCC	5 cut(s)	551, 741, 753, 897, 995
HapII	CCGG	1 cut(s)	545
HgaI	GACGC	1 cut(s)	937
HhaI	GCGC	2 cut(s)	496, 787
Hin1II	CATG	6 cut(s)	171, 250, 310, 621, 1055, 1062
Hin6I	GCGC	2 cut(s)	494, 785
HinP1I	GCGC	2 cut(s)	494, 785
HincII	GTYRAC	1 cut(s)	232
HindII	GTYRAC	1 cut(s)	232
HindIII	AAGCTT	1 cut(s)	534
HinfI	GANTC	7 cut(s)	134, 332, 676, 759, 777, 917, 931
HpaII	CCGG	1 cut(s)	545
HphI	GGTGA	2 cut(s)	823, 953
Hpy166II	GTNNAC	3 cut(s)	210, 232, 359
Hpy188I	TCNGA	3 cut(s)	511, 525, 583
Hpy188III	TCNNGA	1 cut(s)	1021
Hpy8I	GTNNAC	3 cut(s)	210, 232, 359
Hpy99I	CGWCG	2 cut(s)	490, 953
HpyAV	CCTTC	1 cut(s)	349
HpyCH4III	ACNGT	4 cut(s)	363, 644, 859, 889
HpyCH4IV	ACGT	1 cut(s)	1091
HpyCH4V	TGCA	5 cut(s)	81, 578, 731, 1038, 1088
HpyF3I	CTNAG	3 cut(s)	431, 1019, 1076
HpySE526I	ACGT	1 cut(s)	1091
Hsp92II	CATG	6 cut(s)	171, 250, 310, 621, 1055, 1062
HspAI	GCGC	2 cut(s)	494, 785
Kzo9I	GATC	3 cut(s)	264, 511, 816
LmnI	GCTCC	1 cut(s)	712
Lsp1109I	GCAGC	2 cut(s)	275, 715
LweI	GCATC	4 cut(s)	62, 246, 587, 892
MaeI	CTAG	1 cut(s)	404
MaeII	ACGT	1 cut(s)	1091
MaeIII	GTNAC	4 cut(s)	196, 638, 859, 883
MalI	GATC	3 cut(s)	266, 513, 818
MboI	GATC	3 cut(s)	264, 511, 816
MboII	GAAGA	5 cut(s)	19, 22, 25, 217, 364
MfeI	CAATTG	1 cut(s)	376
MflI	RGATCY	1 cut(s)	816
MluCI	AATT	5 cut(s)	104, 376, 474, 744, 1108
MlyI	GAGTC	3 cut(s)	128, 685, 925
MmeI	TCCRAC	1 cut(s)	440
MnlI	CCTC	8 cut(s)	54, 70, 302, 335, 433, 440, 807, 885
Mph1103I	ATGCAT	1 cut(s)	580
MroXI	GAANNNNTTC	1 cut(s)	651
MseI	TTAA	2 cut(s)	102, 597
MslI	CAYNNNNRTG	2 cut(s)	76, 458
MspI	CCGG	1 cut(s)	545
MspR9I	CCNGG	5 cut(s)	153, 314, 755, 765, 1004
MunI	CAATTG	1 cut(s)	376
Mva1269I	GAATGC	2 cut(s)	1040, 1090
MvaI	CCWGG	5 cut(s)	153, 314, 755, 765, 1004
MvnI	CGCG	1 cut(s)	281
NdeII	GATC	3 cut(s)	264, 511, 816
NlaIII	CATG	6 cut(s)	171, 250, 310, 621, 1055, 1062
NmeAIII	GCCGAG	1 cut(s)	1021
NmuCI	GTSAC	2 cut(s)	196, 883
NsiI	ATGCAT	1 cut(s)	580
NspI	RCATGY	2 cut(s)	171, 1062
PasI	CCCWGGG	1 cut(s)	764
PceI	AGGCCT	1 cut(s)	753
PciI	ACATGT	1 cut(s)	1058
PctI	GAATGC	2 cut(s)	1040, 1090
PdmI	GAANNNNTTC	1 cut(s)	651
PfeI	GAWTC	4 cut(s)	332, 759, 777, 917
PflFI	GACNNNGTC	1 cut(s)	254
PflMI	CCANNNNNTGG	1 cut(s)	158
PkrI	GCNGC	3 cut(s)	68, 290, 730
PleI	GAGTC	3 cut(s)	128, 684, 925
PpsI	GAGTC	3 cut(s)	128, 684, 925
PscI	ACATGT	1 cut(s)	1058
PsiI	TTATAA	1 cut(s)	800
Psp6I	CCWGG	5 cut(s)	151, 312, 753, 763, 1002
PspGI	CCWGG	5 cut(s)	151, 312, 753, 763, 1002
PspPI	GGNCC	2 cut(s)	250, 310
PstNI	CAGNNNCTG	1 cut(s)	629
PsuI	RGATCY	1 cut(s)	816
PsyI	GACNNNGTC	1 cut(s)	254
RsaI	GTAC	3 cut(s)	365, 402, 1001
RsaNI	GTAC	3 cut(s)	364, 401, 1000
RseI	CAYNNNNRTG	2 cut(s)	76, 458
SaqAI	TTAA	2 cut(s)	102, 597
SatI	GCNGC	3 cut(s)	67, 289, 729
Sau3AI	GATC	3 cut(s)	264, 511, 816
Sau96I	GGNCC	2 cut(s)	250, 310
ScaI	AGTACT	1 cut(s)	402
SchI	GAGTC	3 cut(s)	128, 685, 925
ScrFI	CCNGG	5 cut(s)	153, 314, 755, 765, 1004
SfaNI	GCATC	4 cut(s)	62, 246, 587, 892
SinI	GGWCC	2 cut(s)	250, 310
SmiMI	CAYNNNNRTG	2 cut(s)	76, 458
SmlI	CTYRAG	1 cut(s)	721
SmoI	CTYRAG	1 cut(s)	721
Sse9I	AATT	5 cut(s)	104, 376, 474, 744, 1108
SseBI	AGGCCT	1 cut(s)	753
SsiI	CCGC	7 cut(s)	57, 66, 127, 279, 301, 336, 845
SspI	AATATT	1 cut(s)	595
SspMI	CTAG	1 cut(s)	404
StuI	AGGCCT	1 cut(s)	753
StyD4I	CCNGG	5 cut(s)	151, 312, 753, 763, 1002
StyI	CCWWGG	1 cut(s)	88
TaaI	ACNGT	4 cut(s)	363, 644, 859, 889
TaiI	ACGT	1 cut(s)	1094
TaqI	TCGA	8 cut(s)	240, 263, 270, 530, 667, 915, 951, 1105
TasI	AATT	5 cut(s)	104, 376, 474, 744, 1108
TatI	WGTACW	2 cut(s)	363, 400
TauI	GCSGC	1 cut(s)	69
TfiI	GAWTC	4 cut(s)	332, 759, 777, 917
Tru1I	TTAA	2 cut(s)	102, 597
Tru9I	TTAA	2 cut(s)	102, 597
TscAI	CASTG	3 cut(s)	399, 630, 894
TseFI	GTSAC	2 cut(s)	196, 883
TseI	GCWGC	2 cut(s)	288, 728
Tsp45I	GTSAC	2 cut(s)	196, 883
TspDTI	ATGAA	4 cut(s)	295, 644, 882, 1040
TspRI	CASTG	3 cut(s)	399, 630, 894
Tth111I	GACNNNGTC	1 cut(s)	254
Van91I	CCANNNNNTGG	1 cut(s)	158
VpaK11BI	GGWCC	2 cut(s)	250, 310
XagI	CCTNNNNNAGG	1 cut(s)	1007
XapI	RAATTY	1 cut(s)	104
XceI	RCATGY	2 cut(s)	171, 1062
XcmI	CCANNNNNNNNNTGG	1 cut(s)	935
XmiI	GTMKAC	1 cut(s)	209
XmnI	GAANNNNTTC	1 cut(s)	651
XspI	CTAG	1 cut(s)	404
ZrmI	AGTACT	1 cut(s)	402
Zsp2I	ATGCAT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.