Rh7BG146500

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000675
Physical Location & Seq
Forward (+)
10888814 .. 10890194
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG146500.1

Sequence Viewer

Length: 1137 bp
ATGGCAACCATAATCACATTACTGCTATTGTTCTTCTTCCTTCCTCACATAGCAGCTACAACCTGCGGCGCATCAGCATGCTCTGCCAGCGGCCCGTCAGTTCGATTCCCATTCCGGCTAACTGATCGCCAGCAAACGGGTTGTGGGTATTCCGGTAAGTTTGGTGTATCGTGCAACGGACACAAACAAACCATTCTTAACCTCCCATCTTCCGGAAACTTCACGGTTGAGAGCATCAGTTACAAGGACCAACTCGTGTGGATCAACGACCTCGACAACTGCATCTTCAAGCGGTTTCTTGACAAGGACTTCAGTCTCATAGGTTCACCCTTCCGGTACGCGCTTGACCTTCAAGAGTACACCTTCTACAAATGTTCGCCCCAACTTGTGCCACTGTGGTCTCCCATCTCCTGTCTCAGCAGTGACAACTATGAGGTCATTGGCGTCGCGAGCTGGTCAACGTTATCGAGATCACCTTCTCCCTATTGTGATGTGATTACCAGAGCTTTGGTTCCAGCAGAAAGTTATGAGTTAGGGAATTCTGACTTTGGTGTGGGGCTGACTTGGAAGGAGCCTGATTGTCGTTCTTGTGAAGCGAGTGGTAAGGTTTGTGGGTTTCAGAATGGTTCACAGTCGAAAATTCACTGCTCTAGTACTCTATCTTATGGTCTCTCAAAAGCTACGAAGTATGGCATAGCGATTGGCGTATTAATTCCAGGGCTATTATCCATTACCTGTTGTGCACTTTATGTCTCAGCCCCGAGAAGGGATCATGGTCGAATCCAAGAATCTACCACCGAATTATCCGGTACTGTAACTAATTATCGTCAACCTCAAGTATTCATAGCAGGCCTTGATGGTCAGACAATTGAGTCATATCCCAAGACTCAACTCGGCGAGAGTTTGGAATTGCCTGACCCTAACGACAAAACTTGTCCAATATGTCTGGGTGAGTACCAACCGAAGGAAACTCTGAGGACTATACCAGAATGTAACCATTATTTCCATGCTAATTGCATTGACGAATGGCTTAGAAAAAATCCGACATGCCCGCTTTGTCGCAATCTACCCGGTCTTTGTCGAAATCCACTGGAGGTGTCATCAGTGAGACCACGTGATGATTATGCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

378

Amino Acids

41.76

Weight (kDa)

6.32

Isoelectric Point (pI)

49.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 22 - 90 5.2e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 309 - 354 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 311 - 354 1.2e-14 Ring finger domain
zf-C3HC4_2 PF13923 312 - 353 2.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 312 - 340 2.5e-09 RING-like zinc finger
zf-C3HC4 PF00097 312 - 353 1.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 312 - 351 6e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 871
Acc36I ACCTGC 1 cut(s) 71
AccII CGCG 2 cut(s) 341, 449
AccIII TCCGGA 1 cut(s) 212
AciI CCGC 4 cut(s) 66, 90, 292, 1052
AclI AACGTT 1 cut(s) 461
AclWI GGATC 2 cut(s) 269, 777
AcsI RAATTY 2 cut(s) 538, 639
AcuI CTGAAG 1 cut(s) 295
AcvI CACGTG 1 cut(s) 1115
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 5 cut(s) 338, 359, 655, 811, 956
AfiI CCNNNNNNNGG 5 cut(s) 136, 212, 765, 766, 964
AgsI TTSAA 2 cut(s) 289, 353
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 4 cut(s) 56, 453, 506, 680
AluI AGCT 4 cut(s) 56, 453, 506, 680
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 6 cut(s) 320, 405, 419, 674, 757, 1102
Alw44I GTGCAC 1 cut(s) 741
AlwI GGATC 2 cut(s) 269, 777
Ama87I CYCGRG 1 cut(s) 760
Aor13HI TCCGGA 1 cut(s) 212
AoxI GGCC 2 cut(s) 91, 850
ApaLI GTGCAC 1 cut(s) 741
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 538, 639
AseI ATTAAT 1 cut(s) 710
AspLEI GCGC 2 cut(s) 71, 343
AspS9I GGNCC 2 cut(s) 92, 247
AsuC2I CCSGG 1 cut(s) 1071
AsuHPI GGTGA 3 cut(s) 318, 465, 962
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 745
BauI CACGAG 1 cut(s) 254
BbrPI CACGTG 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 1 cut(s) 65
BccI CCATC 3 cut(s) 214, 413, 851
BciT130I CCWGG 1 cut(s) 717
BcnI CCSGG 1 cut(s) 1071
BcoDI GTCTC 6 cut(s) 320, 405, 419, 674, 757, 1102
BfaI CTAG 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 71
BisI GCNGC 3 cut(s) 54, 67, 91
BlsI GCNGC 3 cut(s) 55, 68, 92
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 2 cut(s) 717, 1071
Bme18I GGWCC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 2 cut(s) 92, 247
BmiI GGNNCC 2 cut(s) 513, 573
BmrFI CCNGG 2 cut(s) 717, 1071
BmsI GCATC 3 cut(s) 80, 243, 291
BoxI GACNNNNGTC 1 cut(s) 312
BpmI CTGGAG 1 cut(s) 1112
BpuEI CTTGAG 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 1071
BsaAI YACGTR 1 cut(s) 1115
BsaHI GRCGYC 1 cut(s) 444
BsaI GGTCTC 3 cut(s) 405, 674, 1102
BsaJI CCNNGG 1 cut(s) 716
BsaWI WCCGGW 4 cut(s) 152, 212, 333, 806
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 212, 765, 766, 964
Bse1I ACTGG 1 cut(s) 1095
BseAI TCCGGA 1 cut(s) 212
BseBI CCWGG 1 cut(s) 717
BseDI CCNNGG 1 cut(s) 716
BseLI CCNNNNNNNGG 5 cut(s) 136, 212, 765, 766, 964
BseMII CTCAG 3 cut(s) 430, 768, 965
BseNI ACTGG 1 cut(s) 1095
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 1 cut(s) 65
Bsh1236I CGCG 2 cut(s) 341, 449
BshFI GGCC 2 cut(s) 93, 852
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 6 cut(s) 115, 153, 213, 334, 807, 1071
BslI CCNNNNNNNGG 5 cut(s) 136, 212, 765, 766, 964
BsmAI GTCTC 6 cut(s) 320, 405, 419, 674, 757, 1102
BsnI GGCC 2 cut(s) 93, 852
Bso31I GGTCTC 3 cut(s) 405, 674, 1102
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 745
Bsp13I TCCGGA 1 cut(s) 212
Bsp143I GATC 4 cut(s) 124, 261, 470, 769
Bsp68I TCGCGA 1 cut(s) 449
BspACI CCGC 4 cut(s) 66, 90, 292, 1052
BspANI GGCC 2 cut(s) 93, 852
BspCNI CTCAG 3 cut(s) 429, 767, 966
BspEI TCCGGA 1 cut(s) 212
BspFNI CGCG 2 cut(s) 341, 449
BspLI GGNNCC 2 cut(s) 513, 573
BspMI ACCTGC 1 cut(s) 71
BspPI GGATC 2 cut(s) 269, 777
BspTNI GGTCTC 3 cut(s) 405, 674, 1102
BsrI ACTGG 1 cut(s) 1095
BssECI CCNNGG 1 cut(s) 716
BssMI GATC 4 cut(s) 124, 261, 470, 769
BssNI GRCGYC 1 cut(s) 444
BssSI CACGAG 1 cut(s) 254
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 4 cut(s) 226, 396, 633, 814
BstACI GRCGYC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 1115
BstC8I GCNNGC 6 cut(s) 79, 88, 131, 451, 850, 1052
BstDEI CTNAG 4 cut(s) 416, 754, 974, 1031
BstFNI CGCG 2 cut(s) 341, 449
BstHHI GCGC 2 cut(s) 71, 343
BstKTI GATC 4 cut(s) 127, 264, 473, 772
BstMAI GTCTC 6 cut(s) 320, 405, 419, 674, 757, 1102
BstMBI GATC 4 cut(s) 124, 261, 470, 769
BstMWI GCNNNNNNNGC 3 cut(s) 83, 87, 450
BstNI CCWGG 1 cut(s) 717
BstNSI RCATGY 2 cut(s) 81, 1050
BstPAI GACNNNNGTC 1 cut(s) 312
BstSCI CCNGG 2 cut(s) 715, 1069
BstSLI GKGCMC 1 cut(s) 745
BstUI CGCG 2 cut(s) 341, 449
BstV1I GCAGC 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuRI GGCC 2 cut(s) 93, 852
BtsI GCAGTG 2 cut(s) 427, 643
BtsIMutI CAGTG 5 cut(s) 392, 427, 643, 1088, 1110
BtuMI TCGCGA 1 cut(s) 449
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 6 cut(s) 79, 88, 131, 451, 850, 1052
CfoI GCGC 2 cut(s) 71, 343
Cfr13I GGNCC 2 cut(s) 92, 247
CseI GACGC 1 cut(s) 433
Csp6I GTAC 5 cut(s) 337, 358, 654, 810, 955
CspCI CAANNNNNGTGG 2 cut(s) 239, 274
CviAII CATG 4 cut(s) 78, 773, 1007, 1047
CviQI GTAC 5 cut(s) 337, 358, 654, 810, 955
DdeI CTNAG 4 cut(s) 416, 754, 974, 1031
DpnI GATC 4 cut(s) 126, 263, 472, 771
DpnII GATC 4 cut(s) 124, 261, 470, 769
DrdI GACNNNNNNGTC 1 cut(s) 871
DseDI GACNNNNNNGTC 1 cut(s) 871
Eco147I AGGCCT 1 cut(s) 852
Eco31I GGTCTC 3 cut(s) 405, 674, 1102
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 295
Eco72I CACGTG 1 cut(s) 1115
Eco88I CYCGRG 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 715
FaeI CATG 4 cut(s) 81, 776, 1010, 1050
FatI CATG 4 cut(s) 77, 772, 1006, 1046
FauI CCCGC 1 cut(s) 1059
Fnu4HI GCNGC 3 cut(s) 54, 67, 91
Fsp4HI GCNGC 3 cut(s) 54, 67, 91
FspBI CTAG 1 cut(s) 651
GlaI GCGC 2 cut(s) 70, 342
GluI GCNGC 3 cut(s) 54, 67, 91
GsuI CTGGAG 1 cut(s) 1112
HaeIII GGCC 2 cut(s) 93, 852
HapII CCGG 6 cut(s) 115, 153, 213, 334, 807, 1071
HgaI GACGC 1 cut(s) 433
HhaI GCGC 2 cut(s) 71, 343
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 4 cut(s) 81, 776, 1010, 1050
Hin6I GCGC 2 cut(s) 69, 341
HinP1I GCGC 2 cut(s) 69, 341
HincII GTYRAC 2 cut(s) 459, 830
HindII GTYRAC 2 cut(s) 459, 830
HinfI GANTC 5 cut(s) 105, 780, 788, 872, 886
HpaII CCGG 6 cut(s) 115, 153, 213, 334, 807, 1071
HphI GGTGA 3 cut(s) 318, 465, 962
Hpy166II GTNNAC 6 cut(s) 326, 360, 459, 629, 743, 830
Hpy188I TCNGA 5 cut(s) 544, 621, 864, 975, 1044
Hpy188III TCNNGA 5 cut(s) 213, 299, 353, 448, 468
Hpy8I GTNNAC 6 cut(s) 326, 360, 459, 629, 743, 830
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 8 cut(s) 50, 340, 359, 373, 486, 562, 759, 958
HpyCH4III ACNGT 4 cut(s) 226, 396, 633, 814
HpyCH4IV ACGT 2 cut(s) 461, 1114
HpyCH4V TGCA 4 cut(s) 174, 282, 743, 1017
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 87, 450
HpyF3I CTNAG 4 cut(s) 416, 754, 974, 1031
HpySE526I ACGT 2 cut(s) 461, 1114
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 4 cut(s) 81, 776, 1010, 1050
HspAI GCGC 2 cut(s) 69, 341
Kpn2I TCCGGA 1 cut(s) 212
Kzo9I GATC 4 cut(s) 124, 261, 470, 769
LmnI GCTCC 1 cut(s) 571
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 3 cut(s) 80, 243, 291
MaeI CTAG 1 cut(s) 651
MaeII ACGT 2 cut(s) 461, 1114
MaeIII GTNAC 4 cut(s) 239, 422, 814, 992
MalI GATC 4 cut(s) 126, 263, 472, 771
MboI GATC 4 cut(s) 124, 261, 470, 769
MboII GAAGA 4 cut(s) 25, 28, 201, 277
MfeI CAATTG 1 cut(s) 867
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 8 cut(s) 538, 639, 711, 800, 820, 867, 908, 1012
MlyI GAGTC 2 cut(s) 880, 881
MmeI TCCRAC 1 cut(s) 1067
MnlI CCTC 7 cut(s) 54, 212, 281, 427, 843, 969, 1087
MroI TCCGGA 1 cut(s) 212
MseI TTAA 3 cut(s) 198, 710, 1135
MslI CAYNNNNRTG 1 cut(s) 76
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 6 cut(s) 115, 153, 213, 334, 807, 1071
MspR9I CCNGG 2 cut(s) 717, 1071
MunI CAATTG 1 cut(s) 867
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 2 cut(s) 341, 449
MwoI GCNNNNNNNGC 3 cut(s) 83, 87, 450
NciI CCSGG 1 cut(s) 1071
NdeII GATC 4 cut(s) 124, 261, 470, 769
NlaIII CATG 4 cut(s) 81, 776, 1010, 1050
NlaIV GGNNCC 2 cut(s) 513, 573
NmeAIII GCCGAG 1 cut(s) 873
NmuCI GTSAC 1 cut(s) 422
NruI TCGCGA 1 cut(s) 449
NspI RCATGY 2 cut(s) 81, 1050
PaeI GCATGC 1 cut(s) 81
PceI AGGCCT 1 cut(s) 852
PfeI GAWTC 3 cut(s) 105, 780, 788
PkrI GCNGC 3 cut(s) 55, 68, 92
PleI GAGTC 2 cut(s) 880, 880
PmaCI CACGTG 1 cut(s) 1115
PmlI CACGTG 1 cut(s) 1115
PpsI GAGTC 2 cut(s) 880, 880
Ppu21I YACGTR 1 cut(s) 1115
PshAI GACNNNNGTC 1 cut(s) 312
PshBI ATTAAT 1 cut(s) 710
Psp1406I AACGTT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 715
PspCI CACGTG 1 cut(s) 1115
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 2 cut(s) 513, 573
PspPI GGNCC 2 cut(s) 92, 247
RruI TCGCGA 1 cut(s) 449
RsaI GTAC 5 cut(s) 338, 359, 655, 811, 956
RsaNI GTAC 5 cut(s) 337, 358, 654, 810, 955
RseI CAYNNNNRTG 1 cut(s) 76
SaqAI TTAA 3 cut(s) 198, 710, 1135
SatI GCNGC 3 cut(s) 54, 67, 91
Sau3AI GATC 4 cut(s) 124, 261, 470, 769
Sau96I GGNCC 2 cut(s) 92, 247
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 880, 881
ScrFI CCNGG 2 cut(s) 717, 1071
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 3 cut(s) 80, 243, 291
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 834
SmoI CTYRAG 1 cut(s) 834
SphI GCATGC 1 cut(s) 81
Sse9I AATT 8 cut(s) 538, 639, 711, 800, 820, 867, 908, 1012
SseBI AGGCCT 1 cut(s) 852
SsiI CCGC 4 cut(s) 66, 90, 292, 1052
SspMI CTAG 1 cut(s) 651
StuI AGGCCT 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 715, 1069
TaaI ACNGT 4 cut(s) 226, 396, 633, 814
TaiI ACGT 2 cut(s) 464, 1117
TaqI TCGA 6 cut(s) 103, 273, 467, 635, 778, 1081
TasI AATT 8 cut(s) 538, 639, 711, 800, 820, 867, 908, 1012
TatI WGTACW 2 cut(s) 357, 653
TauI GCSGC 2 cut(s) 69, 93
TfiI GAWTC 3 cut(s) 105, 780, 788
Tru1I TTAA 3 cut(s) 198, 710, 1135
Tru9I TTAA 3 cut(s) 198, 710, 1135
TscAI CASTG 5 cut(s) 399, 427, 650, 1095, 1110
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 832
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 5 cut(s) 399, 427, 650, 1095, 1110
VneI GTGCAC 1 cut(s) 741
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 710
XapI RAATTY 2 cut(s) 538, 639
XceI RCATGY 2 cut(s) 81, 1050
XspI CTAG 1 cut(s) 651
ZrmI AGTACT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.