Rw7G032220

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000675
Physical Location & Seq
Forward (+)
46656864 .. 46658225
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032220.1

Sequence Viewer

Length: 1188 bp
ATGTTGGTCTCTGGCCAAATAGTACGTACTGGTTTTAGTTTTCATATGGCAACCTCAGACATATTATTCTCATTCTTCTTCTTCTTCTTCACCTTCTTACTTCCTCAGATAGCAGATAGCAGATGTGGTGGTTCAACATGCTATGGGAGCGGCCCACTGATTCGATTCCCTTTCCGGCTAAGATACCGCCAACCGCTAAATTGTGGGTATTCCATTCATTTTGACCTTTCATGCAACAGTACACGGAAAAACACCATTCTCACCCTCCCTTCTTCAGGAGACTTCGAGGTCGAGAGCATCCATTACAGTGACCAAACCATGTGGATCAACGACCTAAACTGGTGCATGCACAAAAGATTCCTTGACAAAGACTTCAGTCTTGTAGGCACGCCTTTCCATTTCTGGCATAGCCTGGAAAACTATACCTACTTCAAGTGCCCGTCCAAAGTTGTGGCTATTGGGTCTTTTGCACCAATCTCTTGCCTTGGCGATTACAATTACAACGTCATTGCTGTCCCGTCGAGTTGGTCAACGACTGAGAAACCAGCTCCTCAGTCTCTTTGTCGTGCAATTTCCACAGCTTTGCTTCCGAAGAGTTTTGGGTGGGGGGAGTTTGCTGTAAAGTTGACTTGGGGCGTTCCCGATTGTCGTTCTTGTGAAGCAAATGGTAGAGTTTGCAAGCCTAATGTGACAAGTTCCCACATTGAGTGCTCAACTGATGGTATCCCAATATCCGCAAAGACCGGCATAGCAATTGGCGTGTTGATTCCGGCTGGGCTGTTGTGCATTGTTGGATACGCACTCTATGTTGCGGGCAGGAGGGATCATGATGGTCGGGTCCAAGAAACAAACAGGCGTGGTCAGCTTCAAGAACTCATTGAAGCCAATGGACAACCTCAAGTAGTTACAGCGGGTCTTGATGCTCAGACTATTGAGTCATATTTGAAGACTCAACTTGGTGACAGTTTAGAATTACCTGACCCTAATGACAAAACTTGCTCGATATGTTTGGGTGAGTACCAGTCCAAGGAAATGCTAAGGACTATACCAGAATGCAATCATTATTTTCATGTTGGTTGCATAGACGAGTGGCTTAGAAAGAACCCAACATGTCCACTTTGTCGAAATCTTCCTCGAACACCCGAAGTGTCATTAGTGAGACCTCATGATGATCATTCTATCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind zf-RING_2

Protein Analysis

395

Amino Acids

44.21

Weight (kDa)

6.62

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 42 - 111 6.8e-15 Wall-associated receptor kinase galacturonan-binding
zf-rbx1 PF12678 331 - 375 2e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 332 - 375 3.7e-14 Ring finger domain
zf-RING_11 PF17123 333 - 361 7.4e-09 RING-like zinc finger
zf-C3HC4 PF00097 333 - 374 9e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 333 - 374 9.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 287, 934
AccBSI CCGCTC 1 cut(s) 150
AciI CCGC 6 cut(s) 150, 187, 194, 735, 812, 911
AclWI GGATC 2 cut(s) 332, 831
AcoI YGGCCR 1 cut(s) 13
AcuI CTGAAG 2 cut(s) 258, 358
AfaI GTAC 4 cut(s) 24, 28, 241, 1019
AfiI CCNNNNNNNGG 2 cut(s) 275, 1027
AflIII ACRYGT 1 cut(s) 1109
AgsI TTSAA 5 cut(s) 135, 433, 869, 881, 946
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 3 cut(s) 548, 581, 865
AluI AGCT 3 cut(s) 548, 581, 865
Alw21I GWGCWC 1 cut(s) 713
Alw26I GTCTC 4 cut(s) 13, 273, 561, 1153
AlwI GGATC 2 cut(s) 332, 831
AoxI GGCC 2 cut(s) 13, 151
AspS9I GGNCC 2 cut(s) 152, 838
AsuHPI GGTGA 4 cut(s) 82, 253, 971, 1025
AvaII GGWCC 1 cut(s) 838
BaeGI GKGCMC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 713
BccI CCATC 2 cut(s) 713, 824
BciT130I CCWGG 1 cut(s) 413
BciVI GTATCC 2 cut(s) 734, 788
BclI TGATCA 1 cut(s) 1171
BcoDI GTCTC 4 cut(s) 13, 273, 561, 1153
BfuI GTATCC 2 cut(s) 734, 788
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 838
BmgT120I GGNCC 2 cut(s) 152, 838
BmiI GGNNCC 1 cut(s) 839
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 306, 910
BoxI GACNNNNGTC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 1037
BpuEI CTTGAG 1 cut(s) 882
BsaAI YACGTR 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 13, 1153
BsaJI CCNNGG 2 cut(s) 484, 1026
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 1027
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 3 cut(s) 34, 344, 1021
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 484, 1026
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 275, 1027
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 5 cut(s) 69, 119, 528, 566, 938
BseNI ACTGG 3 cut(s) 34, 344, 1021
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 440
BseYI CCCAGC 1 cut(s) 773
BshFI GGCC 2 cut(s) 15, 153
BsiHKAI GWGCWC 1 cut(s) 713
BsiSI CCGG 3 cut(s) 175, 744, 770
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 275, 1027
BsmAI GTCTC 4 cut(s) 13, 273, 561, 1153
BsmFI GGGAC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 1058
BsnI GGCC 2 cut(s) 15, 153
Bso31I GGTCTC 2 cut(s) 13, 1153
Bsp1286I GDGCHC 2 cut(s) 440, 713
Bsp143I GATC 3 cut(s) 324, 823, 1171
BspACI CCGC 6 cut(s) 150, 187, 194, 735, 812, 911
BspANI GGCC 2 cut(s) 15, 153
BspCNI CTCAG 5 cut(s) 68, 118, 529, 565, 937
BspHI TCATGA 2 cut(s) 826, 1165
BspLI GGNNCC 1 cut(s) 839
BspPI GGATC 2 cut(s) 332, 831
BspTNI GGTCTC 2 cut(s) 13, 1153
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 743
BsrI ACTGG 3 cut(s) 34, 344, 1021
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 2 cut(s) 484, 1026
BssMI GATC 3 cut(s) 324, 823, 1171
BssT1I CCWWGG 2 cut(s) 484, 1026
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 3 cut(s) 239, 308, 965
Bst6I CTCTTC 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 26
BstC8I GCNNGC 4 cut(s) 347, 389, 680, 814
BstDEI CTNAG 8 cut(s) 55, 105, 179, 537, 552, 924, 1037, 1094
BstENI CCTNNNNNAGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 297
BstKTI GATC 3 cut(s) 327, 826, 1174
BstMAI GTCTC 4 cut(s) 13, 273, 561, 1153
BstMBI GATC 3 cut(s) 324, 823, 1171
BstMWI GCNNNNNNNGC 2 cut(s) 147, 862
BstNI CCWGG 1 cut(s) 413
BstNSI RCATGY 3 cut(s) 141, 349, 1113
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 440
BstSNI TACGTA 1 cut(s) 26
BstV2I GAAGAC 1 cut(s) 953
BstXI CCANNNNNNTGG 1 cut(s) 451
BsuI GTATCC 2 cut(s) 734, 788
BsuRI GGCC 2 cut(s) 15, 153
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 155, 313
Cac8I GCNNGC 4 cut(s) 347, 389, 680, 814
CciI TCATGA 2 cut(s) 826, 1165
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 2 cut(s) 152, 838
Csp6I GTAC 4 cut(s) 23, 27, 240, 1018
CspCI CAANNNNNGTGG 2 cut(s) 302, 337
CviAII CATG 8 cut(s) 138, 231, 319, 346, 827, 1070, 1110, 1166
CviQI GTAC 4 cut(s) 23, 27, 240, 1018
DdeI CTNAG 8 cut(s) 55, 105, 179, 537, 552, 924, 1037, 1094
DpnI GATC 3 cut(s) 326, 825, 1173
DpnII GATC 3 cut(s) 324, 823, 1171
DrdI GACNNNNNNGTC 2 cut(s) 287, 934
DseDI GACNNNNNNGTC 2 cut(s) 287, 934
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Eco105I TACGTA 1 cut(s) 26
Eco130I CCWWGG 2 cut(s) 484, 1026
Eco31I GGTCTC 2 cut(s) 13, 1153
Eco47I GGWCC 1 cut(s) 838
Eco57I CTGAAG 2 cut(s) 258, 358
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 2 cut(s) 484, 1026
ErhI CCWWGG 2 cut(s) 484, 1026
FaeI CATG 8 cut(s) 141, 234, 322, 349, 830, 1073, 1113, 1169
FaqI GGGAC 1 cut(s) 500
FatI CATG 8 cut(s) 137, 230, 318, 345, 826, 1069, 1109, 1165
FauI CCCGC 2 cut(s) 805, 904
FauNDI CATATG 1 cut(s) 45
FbaI TGATCA 1 cut(s) 1171
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 151
GluI GCNGC 1 cut(s) 151
GsaI CCCAGC 1 cut(s) 777
HaeIII GGCC 2 cut(s) 15, 153
HapII CCGG 3 cut(s) 175, 744, 770
Hin1II CATG 8 cut(s) 141, 234, 322, 349, 830, 1073, 1113, 1169
HincII GTYRAC 2 cut(s) 531, 627
HindII GTYRAC 2 cut(s) 531, 627
HinfI GANTC 6 cut(s) 160, 165, 357, 766, 935, 949
HpaII CCGG 3 cut(s) 175, 744, 770
HphI GGTGA 4 cut(s) 82, 253, 971, 1025
Hpy166II GTNNAC 4 cut(s) 242, 531, 627, 1115
Hpy188I TCNGA 4 cut(s) 58, 108, 591, 927
Hpy188III TCNNGA 7 cut(s) 276, 292, 641, 827, 869, 917, 1166
Hpy8I GTNNAC 4 cut(s) 242, 531, 627, 1115
Hpy99I CGWCG 1 cut(s) 523
HpyAV CCTTC 2 cut(s) 103, 279
HpyCH4III ACNGT 3 cut(s) 239, 308, 965
HpyCH4IV ACGT 2 cut(s) 25, 504
HpyCH4V TGCA 9 cut(s) 234, 345, 349, 470, 569, 678, 786, 1056, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 862
HpyF3I CTNAG 8 cut(s) 55, 105, 179, 537, 552, 924, 1037, 1094
HpySE526I ACGT 2 cut(s) 25, 504
Hsp92II CATG 8 cut(s) 141, 234, 322, 349, 830, 1073, 1113, 1169
Ksp22I TGATCA 1 cut(s) 1171
Kzo9I GATC 3 cut(s) 324, 823, 1171
LmnI GCTCC 2 cut(s) 147, 553
LweI GCATC 2 cut(s) 306, 910
MaeII ACGT 2 cut(s) 25, 504
MaeIII GTNAC 4 cut(s) 308, 688, 904, 959
MalI GATC 3 cut(s) 326, 825, 1173
MbiI CCGCTC 1 cut(s) 150
MboI GATC 3 cut(s) 324, 823, 1171
MboII GAAGA 9 cut(s) 67, 70, 73, 76, 79, 264, 604, 958, 1121
MfeI CAATTG 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 440, 713
MlsI TGGCCA 1 cut(s) 15
MluCI AATT 5 cut(s) 199, 496, 570, 753, 971
MluNI TGGCCA 1 cut(s) 15
MlyI GAGTC 2 cut(s) 943, 944
MmeI TCCRAC 1 cut(s) 772
MnlI CCTC 9 cut(s) 64, 114, 275, 280, 561, 813, 906, 1143, 1173
Mox20I TGGCCA 1 cut(s) 15
MscI TGGCCA 1 cut(s) 15
MseI TTAA 1 cut(s) 1186
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 3 cut(s) 175, 744, 770
MspR9I CCNGG 1 cut(s) 413
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 1 cut(s) 1058
MvaI CCWGG 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 147, 862
NdeI CATATG 1 cut(s) 45
NdeII GATC 3 cut(s) 324, 823, 1171
NlaIII CATG 8 cut(s) 141, 234, 322, 349, 830, 1073, 1113, 1169
NlaIV GGNNCC 1 cut(s) 839
NmuCI GTSAC 3 cut(s) 308, 688, 959
NspI RCATGY 3 cut(s) 141, 349, 1113
PaeI GCATGC 1 cut(s) 349
PagI TCATGA 2 cut(s) 826, 1165
PciI ACATGT 1 cut(s) 1109
PctI GAATGC 1 cut(s) 1058
PfeI GAWTC 4 cut(s) 160, 165, 357, 766
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 2 cut(s) 943, 943
PpsI GAGTC 2 cut(s) 943, 943
Ppu21I YACGTR 1 cut(s) 26
PscI ACATGT 1 cut(s) 1109
PshAI GACNNNNGTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 773
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 839
PspPI GGNCC 2 cut(s) 152, 838
RsaI GTAC 4 cut(s) 24, 28, 241, 1019
RsaNI GTAC 4 cut(s) 23, 27, 240, 1018
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 1 cut(s) 1186
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 3 cut(s) 324, 823, 1171
Sau96I GGNCC 2 cut(s) 152, 838
SchI GAGTC 2 cut(s) 943, 944
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 440, 713
SfaNI GCATC 2 cut(s) 306, 910
SinI GGWCC 1 cut(s) 838
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 897
SmoI CTYRAG 1 cut(s) 897
SnaBI TACGTA 1 cut(s) 26
SphI GCATGC 1 cut(s) 349
Sse9I AATT 5 cut(s) 199, 496, 570, 753, 971
SsiI CCGC 6 cut(s) 150, 187, 194, 735, 812, 911
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 2 cut(s) 484, 1026
TaaI ACNGT 3 cut(s) 239, 308, 965
TaiI ACGT 2 cut(s) 28, 507
TaqI TCGA 7 cut(s) 163, 285, 291, 521, 1001, 1123, 1135
TasI AATT 5 cut(s) 199, 496, 570, 753, 971
TatI WGTACW 1 cut(s) 239
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 4 cut(s) 160, 165, 357, 766
Tru1I TTAA 1 cut(s) 1186
Tru9I TTAA 1 cut(s) 1186
TscAI CASTG 2 cut(s) 162, 313
TseFI GTSAC 3 cut(s) 308, 688, 959
Tsp45I GTSAC 3 cut(s) 308, 688, 959
TspDTI ATGAA 4 cut(s) 32, 206, 219, 1058
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 2 cut(s) 162, 313
VpaK11BI GGWCC 1 cut(s) 838
XagI CCTNNNNNAGG 1 cut(s) 273
XceI RCATGY 3 cut(s) 141, 349, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.