MD02G1018900.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000413
Physical Location & Seq
Forward (+)
1376358 .. 1378964
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1018900.v1.1.491

Sequence Viewer

Length: 2562 bp
ATGGTCACTCAGATAGAACTCCATAGCCTAAACACTTGCAAAATTCAGCAACTCCATCAAATTTTCATGGAAAATCTTCCTATCCGTAAAACCCCTCCCCCTCTATTTTCCGTGCTTGTTTTTCTCCTCCATTTCTCATCCCTTCACTTTCCCTCGTTGGCTTACGATACCCCTGATAAGTACTTCATCAACTGTGGGTCAAATGCTAATGCCAACTTCAATAACCATGTCTTCACTGCAGATCGTTTCTTCCACTCGAAGGCGACAAGCAGTATCAATGGCGGCAACCAGTCGAATCTTTACCTTACAGCAAGAATTTTCGAGCAAGAATCCTACTATAAGTTCGACATCACTGAAAACGGTACTTATTATGTACGTCTGCATTTCTTGGCTTTCGCCTCTTCCTCAAGTAATCTCTCCGCTGCTATTTTTGATGTTTCGGCTTTTCCTAATAATTCAAATTCTGGATTCATGCTGTTGAAGAATTTCACTGCTAAGAATAGTAGTGGCAATTCTACGATACATGAGTTCTTTCTTGGCATTGATCCTGGCTCATTTAGGATATACTTTACTCCTCGTGGATTGTCTTTTGCATTTGTAAATGCCATTGAAGTCTTCCTTGCCCCTGCAAATTTCAGCCCTGAGAATTACCCAAGTAGTTCCCCATTAGTTCTACATACAATTTACAGGGTGAATGTTGGAGGTCATGAACTCGGACCAGATGAGGACAAACTATGGCGAAACTGGGAACCTGACGATCATTATCTGTTGAATTCAAACCCTGTAGGGGAAGTTGGACCCTCACAGAAGCCTAGGTACTTGGAGTCTAAAAATGATGGTTTTGTTGCTGCTGCTAATTATTTTATTGCCCCAGATTTAGTTTACGAGACTGCCAAAGTAATGAACAATGGTAGTAGAAACCCATCCACTTCGTTCAACATAACCTGGTCGTTTAATGTGCGCAGGAATGCTAAACATCTTGTCCGAGCACACTTTTGTGACCTTGTTGGCCAAGCTGCCGACATTGTTTTTAACTTGTATTCAAATGGAAACTTCATTAAGAATGTCAATAATTCTGTGTTTGTCAACCCGATACCTTACTACTATGATTTTGTGGTGGAGTCTAAGGAGTCTGAACTCATTAACATCAGCATAGGTCCTAATGTAGAAAAAACTTCCACTAACAATTCCTTTCTAAATGGACTGGAAATGTTGGAAATAATGGATGAATTGGCACAAATTCCAAATATGAAAGAGCACAAGAGCATAAATGTCCCTGTTGTGGTTGGTTCAGTTCTTGGAGGTCTGTCTCTTATCTGCATTTTGGTTGTCGGGTTCTTGTTCGGTTTGAAATACAGAAAGGCAAAGCATGTTGAAACTTCGGAATGGTCACCAATGCCTGCATTTGGGGGAGGGAGTACCCACAGTAGGGTCACTGAGGGAACGATTACTGGCTCCCCTATGACTTATCTAAATCTTGGGTTGAAGATATCTTTTGCTCAACTTCAGCAAGCAACGAACAACTTTGACACAAAATTTCTGATAGGCAAGGGTGGCTTTGGGAACGTCTATAGGGGAACTCTATCAGATGGGAGAATTGTAGCTGTCAAGCGAGGTAAGCGACATGAACATAGTTCAGGTCAAGGGCTTCCAGAGTTTGAAACAGAGATAATGGTTTTGTCCAGGATTCGTCATCGCCATCTTGTCTCCTTAATTGGGTACTGTGATGAAGGGTCTGAGATGATATTGGTCTACGAGTTCATGGAAAAGGGGACCTTGAGAGAACATTTATATGAATCAAATTTGCCTCGCTTGCCATGGAAGCAAAGACTTGAAATCTGCATTGGTGCAGCAAGGGGTCTTCATTACCTCCACAAGGGTGCAGCCGGAGGCATCATTCACCGCGATGTTAAGTCCACCAACATCTTGTTGGATGCAAAATGTGTTGCCAAAGTTGCTGATTTTGGCCTTTCAAGATCTGGTCCACTTGATGAAACCCATGTCAGCACAAATGTCAAAGGCACTTTTGGTTACCTAGATCCTGAGTACATACTGACTCAACAGTTGACAGAAAAATCAGATGTTTACTCATTCGGCGTTGTTCTCCTTGAGGTCTTATGTGCAAGACCTGCTCTGGCTAGGAATCTACCAAATGAGCAAATAAATTTAGCGGAATGGGGAATGAATTGCAAGAAGAACGGATTGCTTGAACAGATTGTTGATTCTTCACTCAAGGGTCAGATTGATCCTAGCTCGCTAAGAAAATTCATCGAGACAGCAGAGAAATGCTTGCAAGATGATGCTAGTGATAGGCCTACAATGGATGATGTACTGTGGGACTTGGACTATGCGTTACAGCTCCAGCAAACCGCAAAGCATAGAGAGCCCCACGAGGACAGCACAATCAATGCTTCATCAGAATTCGTATTGCCAAATGTTCAGCATTTTCCTTCCCATAGCTCTACAGCTAACCCAGATGATATGACCTTTTCCGGGGTGGATGAATCGGACACGGCAACAAACAAAGTATTCTCACAACTGAAGATTGGTGATGCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

854

Amino Acids

95.19

Weight (kDa)

6.09

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 61 - 146 3.3e-07 Malectin domain
Malectin_like PF12819 63 - 405 1.6e-24 Malectin-like domain
Pkinase PF00069 512 - 778 5.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 514 - 779 2.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23200
fragaria_vesca FvH4_1g16860 FvH4_1g16861
malus_domestica MD02G1018900.v1.1 MD15G1289500.v1.1 MD15G1289600.v1.1 MD15G1289700.v1.1
prunus_persica Prupe.6G227800_v2.0.a1 Prupe.6G228000_v2.0.a1 Prupe.6G228100_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228300_v2.0.a1
pyrus_communis pycom02g01680 pycom02g01690 pycom15g25310 pycom15g25330 pycom15g25340
rosa_chinensis RchiOBHm_Chr1g0324321 RchiOBHm_Chr1g0324331 RchiOBHm_Chr1g0324341 RchiOBHm_Chr1g0324401 RchiOBHm_Chr1g0324441 RchiOBHm_Chr2g0106751 RchiOBHm_Chr2g0106761 RchiOBHm_Chr2g0106771 RchiOBHm_Chr2g0106781 RchiOBHm_Chr2g0106801 RchiOBHm_Chr2g0106811 RchiOBHm_Chr2g0106821
rosa_laevigata RLG00000017539 RLG00000017540 RLG00000017541 RLG00000030200 RLG00000030201
rosa_multiflora Rmu_sc0000751.1_g000001 Rmu_sc0000751.1_g000002 Rmu_sc0000751.1_g000003 Rmu_sc0000751.1_g000005 Rmu_sc0000751.1_g000007 Rmu_sc0000751.1_g000008 Rmu_sc0000751.1_g000018 Rmu_sc0000751.1_g000019 Rmu_sc0000751.1_g000026 Rmu_sc0000751.1_g000027 Rmu_sc0000751.1_g000028 Rmu_sc0000751.1_g000029 Rmu_sc0003410.1_g000012 Rmu_sc0004000.1_g000023 Rmu_sc0004000.1_g000024 Rmu_sc0004082.1_g000015 Rmu_sc0004082.1_g000017 Rmu_sc0026507.1_g000001 Rmu_sc0031196.1_g000001 Rmu_sc0039591.1_g000001
rosa_roxburghii Rroxscaffold_159G00432710 Rroxscaffold_159G00432780 Rroxscaffold_159G00432840 Rroxscaffold_159G00433010 Rroxscaffold_2G00098070 Rroxscaffold_2G00136770 Rroxscaffold_4G00325540 Rroxscaffold_4G00325630 Rroxscaffold_4G00325680
rosa_rugosa Rorug01G0045700 Rorug02G0138500 Rorug02G0138600 Rorug02G0138700 Rorug02G0138800 Rorug02G0138900
rosa_samantha Rh1AG061400 Rh1BG052300 Rh1CG063400 Rh1CG063700 Rh1DG067800 Rh2AG189400 Rh2AG189800 Rh2AG190000 Rh2AG190100 Rh2BG201100 Rh2BG201300 Rh2CG194500 Rh2CG194600 Rh2CG194700 Rh2DG195700 Rh2DG195900 Rh2DG196300 Rh2DG196400 Rh2DG196500 Rh2DG196600
rosa_wichuraiana Rw1G004230 Rw1G005210 Rw2G014900 Rw2G014910 Rw2G014930 Rw2G014940 Rw2G014950 Rw2G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 962
Acc36I ACCTGC 1 cut(s) 2137
AccI GTMKAC 1 cut(s) 1752
AccII CGCG 1 cut(s) 1905
AciI CCGC 5 cut(s) 282, 420, 1903, 2171, 2370
AclWI GGATC 3 cut(s) 539, 2033, 2240
AcoI YGGCCR 1 cut(s) 1009
AcuI CTGAAG 2 cut(s) 1490, 2561
AfaI GTAC 8 cut(s) 182, 364, 375, 818, 1420, 1721, 2048, 2331
AfiI CCNNNNNNNGG 9 cut(s) 259, 787, 1282, 1406, 1428, 1429, 1716, 1876, 2493
AjnI CCWGG 3 cut(s) 547, 944, 1682
AjuI GAANNNNNNNTTGG 2 cut(s) 1827, 1859
AleI CACNNNNGTG 2 cut(s) 996, 1878
AluBI AGCT 6 cut(s) 1016, 1604, 2253, 2359, 2460, 2468
AluI AGCT 6 cut(s) 1016, 1604, 2253, 2359, 2460, 2468
Alw21I GWGCWC 2 cut(s) 991, 1260
Alw26I GTCTC 4 cut(s) 881, 1314, 1711, 2267
AlwI GGATC 3 cut(s) 539, 2033, 2240
AoxI GGCC 3 cut(s) 1009, 1966, 2312
ApeKI GCWGC 6 cut(s) 422, 848, 851, 1016, 1850, 1883
Asp700I GAANNNNTTC 2 cut(s) 75, 485
AspA2I CCTAGG 1 cut(s) 812
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 5 cut(s) 716, 797, 1157, 1773, 1982
AsuC2I CCSGG 1 cut(s) 2494
AsuHPI GGTGA 4 cut(s) 703, 1383, 1892, 2561
AvaII GGWCC 5 cut(s) 716, 797, 1157, 1773, 1982
AvrII CCTAGG 1 cut(s) 812
BaeI ACNNNNGTAYC 2 cut(s) 512, 545
BalI TGGCCA 1 cut(s) 1011
BanII GRGCYC 1 cut(s) 2388
BarI GAAGNNNNNNTAC 2 cut(s) 800, 832
BauI CACGAG 2 cut(s) 576, 2390
BbsI GAAGAC 3 cut(s) 223, 607, 1853
Bbv12I GWGCWC 2 cut(s) 991, 1260
BbvI GCAGC 6 cut(s) 409, 835, 838, 1003, 1862, 1895
BccI CCATC 5 cut(s) 63, 830, 931, 1583, 1707
BceAI ACGGC 1 cut(s) 2529
BciT130I CCWGG 3 cut(s) 549, 946, 1684
BcnI CCSGG 1 cut(s) 2494
BcoDI GTCTC 4 cut(s) 881, 1314, 1711, 2267
BfaI CTAG 5 cut(s) 813, 2036, 2139, 2250, 2304
BfmI CTRYAG 4 cut(s) 237, 783, 1570, 2463
BfuAI ACCTGC 1 cut(s) 2137
BglII AGATCT 1 cut(s) 1976
BisI GCNGC 7 cut(s) 283, 423, 849, 852, 1017, 1851, 1884
BlnI CCTAGG 1 cut(s) 812
BlsI GCNGC 7 cut(s) 284, 424, 850, 853, 1018, 1852, 1885
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 4 cut(s) 549, 946, 1684, 2494
Bme18I GGWCC 5 cut(s) 716, 797, 1157, 1773, 1982
BmgT120I GGNCC 5 cut(s) 716, 797, 1157, 1773, 1982
BmiI GGNNCC 4 cut(s) 750, 799, 1456, 1774
BmrFI CCNGG 4 cut(s) 549, 946, 1684, 2494
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 4 cut(s) 1902, 1924, 2290, 2542
BmuI ACTGGG 1 cut(s) 754
BpiI GAAGAC 3 cut(s) 223, 607, 1853
BpmI CTGGAG 1 cut(s) 2345
BpuEI CTTGAG 4 cut(s) 391, 1798, 2129, 2216
BpuMI CCSGG 1 cut(s) 2494
BsaBI GATNNNNATC 1 cut(s) 762
BsaJI CCNNGG 3 cut(s) 812, 1817, 2493
Bsc4I CCNNNNNNNGG 9 cut(s) 259, 787, 1282, 1406, 1428, 1429, 1716, 1876, 2493
Bse1I ACTGG 4 cut(s) 289, 749, 1209, 1456
Bse8I GATNNNNATC 1 cut(s) 762
BseBI CCWGG 3 cut(s) 549, 946, 1684
BseDI CCNNGG 3 cut(s) 812, 1817, 2493
BseGI GGATG 6 cut(s) 137, 923, 1231, 1939, 2329, 2506
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 9 cut(s) 259, 787, 1282, 1406, 1428, 1429, 1716, 1876, 2493
BseMII CTCAG 5 cut(s) 23, 633, 1428, 1728, 2034
BseNI ACTGG 4 cut(s) 289, 749, 1209, 1456
BseRI GAGGAG 2 cut(s) 116, 564
BseXI GCAGC 6 cut(s) 409, 835, 838, 1003, 1862, 1895
BsgI GTGCAG 2 cut(s) 1869, 1902
Bsh1236I CGCG 1 cut(s) 1905
BshFI GGCC 3 cut(s) 1011, 1968, 2314
BsiHKAI GWGCWC 2 cut(s) 991, 1260
BsiSI CCGG 2 cut(s) 1887, 2493
BslFI GGGAC 3 cut(s) 1259, 1786, 2351
BslI CCNNNNNNNGG 9 cut(s) 259, 787, 1282, 1406, 1428, 1429, 1716, 1876, 2493
BsmAI GTCTC 4 cut(s) 881, 1314, 1711, 2267
BsmFI GGGAC 3 cut(s) 1259, 1786, 2351
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 3 cut(s) 1011, 1968, 2314
Bsp1286I GDGCHC 3 cut(s) 991, 1260, 2388
Bsp143I GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
Bsp19I CCATGG 1 cut(s) 1817
BspACI CCGC 5 cut(s) 282, 420, 1903, 2171, 2370
BspANI GGCC 3 cut(s) 1011, 1968, 2314
BspCNI CTCAG 5 cut(s) 22, 634, 1429, 1729, 2035
BspFNI CGCG 1 cut(s) 1905
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 4 cut(s) 750, 799, 1456, 1774
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 2137
BspPI GGATC 3 cut(s) 539, 2033, 2240
BsrI ACTGG 4 cut(s) 289, 749, 1209, 1456
BssECI CCNNGG 3 cut(s) 812, 1817, 2493
BssMI GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
BssSI CACGAG 2 cut(s) 576, 2390
BssT1I CCWWGG 2 cut(s) 812, 1817
Bst2BI CACGAG 2 cut(s) 576, 2390
Bst2UI CCWGG 3 cut(s) 549, 946, 1684
Bst4CI ACNGT 6 cut(s) 194, 362, 1427, 1724, 2064, 2334
Bst6I CTCTTC 1 cut(s) 406
BstAPI GCANNNNNTGC 1 cut(s) 2129
BstC8I GCNNGC 5 cut(s) 1401, 1512, 1814, 2255, 2291
BstDEI CTNAG 8 cut(s) 9, 495, 642, 1125, 1437, 1737, 2043, 2258
BstDSI CCRYGG 1 cut(s) 1817
BstEII GGTNACC 2 cut(s) 1389, 2030
BstENI CCTNNNNNAGG 1 cut(s) 1874
BstF5I GGATG 6 cut(s) 137, 923, 1231, 1939, 2329, 2506
BstFNI CGCG 1 cut(s) 1905
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 6 cut(s) 244, 547, 760, 1979, 2041, 2248
BstMAI GTCTC 4 cut(s) 881, 1314, 1711, 2267
BstMBI GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
BstMWI GCNNNNNNNGC 7 cut(s) 1554, 1618, 1813, 1822, 1955, 2129, 2383
BstNI CCWGG 3 cut(s) 549, 946, 1684
BstNSI RCATGY 1 cut(s) 1373
BstPI GGTNACC 2 cut(s) 1389, 2030
BstSCI CCNGG 4 cut(s) 547, 944, 1682, 2492
BstSFI CTRYAG 4 cut(s) 237, 783, 1570, 2463
BstUI CGCG 1 cut(s) 1905
BstV1I GCAGC 6 cut(s) 409, 835, 838, 1003, 1862, 1895
BstV2I GAAGAC 3 cut(s) 223, 607, 1853
BstX2I RGATCY 2 cut(s) 1976, 2038
BstYI RGATCY 2 cut(s) 1976, 2038
BsuRI GGCC 3 cut(s) 1011, 1968, 2314
BtgI CCRYGG 1 cut(s) 1817
BtgZI GCGATG 2 cut(s) 1679, 1920
BtsCI GGATG 6 cut(s) 137, 923, 1231, 1939, 2329, 2506
BtsI GCAGTG 2 cut(s) 234, 489
BtsIMutI CAGTG 4 cut(s) 234, 351, 489, 1434
BveI ACCTGC 1 cut(s) 2137
Cac8I GCNNGC 5 cut(s) 1401, 1512, 1814, 2255, 2291
CciI TCATGA 1 cut(s) 706
CfoI GCGC 1 cut(s) 963
Cfr13I GGNCC 5 cut(s) 716, 797, 1157, 1773, 1982
CsiI ACCWGGT 1 cut(s) 944
Csp6I GTAC 8 cut(s) 181, 363, 374, 817, 1419, 1720, 2047, 2330
CviQI GTAC 8 cut(s) 181, 363, 374, 817, 1419, 1720, 2047, 2330
DdeI CTNAG 8 cut(s) 9, 495, 642, 1125, 1437, 1737, 2043, 2258
DpnI GATC 6 cut(s) 243, 546, 759, 1978, 2040, 2247
DpnII GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
EaeI YGGCCR 1 cut(s) 1009
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
Eco130I CCWWGG 2 cut(s) 812, 1817
Eco147I AGGCCT 1 cut(s) 2314
Eco24I GRGCYC 1 cut(s) 2388
Eco32I GATATC 1 cut(s) 1491
Eco47I GGWCC 5 cut(s) 716, 797, 1157, 1773, 1982
Eco57I CTGAAG 2 cut(s) 1490, 2561
Eco91I GGTNACC 2 cut(s) 1389, 2030
EcoNI CCTNNNNNAGG 1 cut(s) 1874
EcoO109I RGGNCCY 2 cut(s) 1157, 1773
EcoO65I GGTNACC 2 cut(s) 1389, 2030
EcoRI GAATTC 2 cut(s) 772, 2420
EcoRII CCWGG 3 cut(s) 547, 944, 1682
EcoRV GATATC 1 cut(s) 1491
EcoT14I CCWWGG 2 cut(s) 812, 1817
EcoT38I GRGCYC 1 cut(s) 2388
ErhI CCWWGG 2 cut(s) 812, 1817
FalI AAGNNNNNCTT 6 cut(s) 603, 635, 1541, 1573, 1760, 1792
FaqI GGGAC 3 cut(s) 1259, 1786, 2351
FblI GTMKAC 1 cut(s) 1752
Fnu4HI GCNGC 7 cut(s) 283, 423, 849, 852, 1017, 1851, 1884
FokI GGATG 6 cut(s) 124, 910, 1238, 1946, 2336, 2513
FriOI GRGCYC 1 cut(s) 2388
Fsp4HI GCNGC 7 cut(s) 283, 423, 849, 852, 1017, 1851, 1884
FspBI CTAG 5 cut(s) 813, 2036, 2139, 2250, 2304
FspI TGCGCA 1 cut(s) 962
GlaI GCGC 1 cut(s) 962
GluI GCNGC 7 cut(s) 283, 423, 849, 852, 1017, 1851, 1884
GsuI CTGGAG 1 cut(s) 2345
HaeIII GGCC 3 cut(s) 1011, 1968, 2314
HapII CCGG 2 cut(s) 1887, 2493
HhaI GCGC 1 cut(s) 963
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HincII GTYRAC 2 cut(s) 1087, 2067
HindII GTYRAC 2 cut(s) 1087, 2067
HpaII CCGG 2 cut(s) 1887, 2493
HphI GGTGA 4 cut(s) 703, 1383, 1892, 2561
Hpy166II GTNNAC 7 cut(s) 883, 1087, 1753, 1917, 1985, 2067, 2086
Hpy188III TCNNGA 6 cut(s) 465, 707, 1652, 1974, 2042, 2272
Hpy8I GTNNAC 7 cut(s) 883, 1087, 1753, 1917, 1985, 2067, 2086
HpyAV CCTTC 4 cut(s) 152, 253, 1724, 2460
HpyCH4III ACNGT 6 cut(s) 194, 362, 1427, 1724, 2064, 2334
HpyCH4IV ACGT 2 cut(s) 376, 1566
HpyF10VI GCNNNNNNNGC 7 cut(s) 1554, 1618, 1813, 1822, 1955, 2129, 2383
HpyF3I CTNAG 8 cut(s) 9, 495, 642, 1125, 1437, 1737, 2043, 2258
HpySE526I ACGT 2 cut(s) 376, 1566
HspAI GCGC 1 cut(s) 961
Kzo9I GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
LmnI GCTCC 2 cut(s) 1460, 2364
Lsp1109I GCAGC 6 cut(s) 409, 835, 838, 1003, 1862, 1895
LweI GCATC 4 cut(s) 1902, 1924, 2290, 2542
MabI ACCWGGT 1 cut(s) 944
MaeI CTAG 5 cut(s) 813, 2036, 2139, 2250, 2304
MaeII ACGT 2 cut(s) 376, 1566
MaeIII GTNAC 6 cut(s) 4, 998, 1389, 1432, 2030, 2352
MalI GATC 6 cut(s) 243, 546, 759, 1978, 2040, 2247
MboI GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
MflI RGATCY 2 cut(s) 1976, 2038
MhlI GDGCHC 3 cut(s) 991, 1260, 2388
MlsI TGGCCA 1 cut(s) 1011
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 4 cut(s) 833, 1130, 1139, 2050
MmeI TCCRAC 4 cut(s) 679, 775, 1194, 1911
Mox20I TGGCCA 1 cut(s) 1011
MroXI GAANNNNTTC 2 cut(s) 75, 485
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 6 cut(s) 954, 1032, 1059, 1143, 1712, 1911
MslI CAYNNNNRTG 4 cut(s) 996, 1791, 1878, 1905
Msp20I TGGCCA 1 cut(s) 1011
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 2 cut(s) 1887, 2493
MspR9I CCNGG 4 cut(s) 549, 946, 1684, 2494
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 3 cut(s) 549, 946, 1684
MvnI CGCG 1 cut(s) 1905
MwoI GCNNNNNNNGC 7 cut(s) 1554, 1618, 1813, 1822, 1955, 2129, 2383
NciI CCSGG 1 cut(s) 2494
NcoI CCATGG 1 cut(s) 1817
NdeII GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
NlaIV GGNNCC 4 cut(s) 750, 799, 1456, 1774
NmuCI GTSAC 4 cut(s) 4, 998, 1389, 1432
NsbI TGCGCA 1 cut(s) 962
NspI RCATGY 1 cut(s) 1373
OliI CACNNNNGTG 2 cut(s) 996, 1878
PagI TCATGA 1 cut(s) 706
PceI AGGCCT 1 cut(s) 2314
PctI GAATGC 1 cut(s) 973
PdmI GAANNNNTTC 2 cut(s) 75, 485
PfeI GAWTC 8 cut(s) 295, 329, 468, 1687, 1796, 2143, 2222, 2504
PfoI TCCNGGA 1 cut(s) 1682
PkrI GCNGC 7 cut(s) 284, 424, 850, 853, 1018, 1852, 1885
PleI GAGTC 4 cut(s) 832, 1129, 1138, 2050
PpsI GAGTC 4 cut(s) 832, 1129, 1138, 2050
PpuMI RGGWCCY 2 cut(s) 1157, 1773
Psp5II RGGWCCY 2 cut(s) 1157, 1773
Psp6I CCWGG 3 cut(s) 547, 944, 1682
PspEI GGTNACC 2 cut(s) 1389, 2030
PspGI CCWGG 3 cut(s) 547, 944, 1682
PspN4I GGNNCC 4 cut(s) 750, 799, 1456, 1774
PspPI GGNCC 5 cut(s) 716, 797, 1157, 1773, 1982
PspPPI RGGWCCY 2 cut(s) 1157, 1773
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 1976, 2038
RsaI GTAC 8 cut(s) 182, 364, 375, 818, 1420, 1721, 2048, 2331
RsaNI GTAC 8 cut(s) 181, 363, 374, 817, 1419, 1720, 2047, 2330
RseI CAYNNNNRTG 4 cut(s) 996, 1791, 1878, 1905
SaqAI TTAA 6 cut(s) 954, 1032, 1059, 1143, 1712, 1911
SatI GCNGC 7 cut(s) 283, 423, 849, 852, 1017, 1851, 1884
Sau3AI GATC 6 cut(s) 241, 544, 757, 1976, 2038, 2245
Sau96I GGNCC 5 cut(s) 716, 797, 1157, 1773, 1982
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 4 cut(s) 833, 1130, 1139, 2050
ScrFI CCNGG 4 cut(s) 549, 946, 1684, 2494
SduI GDGCHC 3 cut(s) 991, 1260, 2388
SexAI ACCWGGT 1 cut(s) 944
SfaNI GCATC 4 cut(s) 1902, 1924, 2290, 2542
SfcI CTRYAG 4 cut(s) 237, 783, 1570, 2463
SinI GGWCC 5 cut(s) 716, 797, 1157, 1773, 1982
SmiMI CAYNNNNRTG 4 cut(s) 996, 1791, 1878, 1905
SmlI CTYRAG 4 cut(s) 406, 1777, 2108, 2231
SmoI CTYRAG 4 cut(s) 406, 1777, 2108, 2231
SseBI AGGCCT 1 cut(s) 2314
SsiI CCGC 5 cut(s) 282, 420, 1903, 2171, 2370
SspMI CTAG 5 cut(s) 813, 2036, 2139, 2250, 2304
StuI AGGCCT 1 cut(s) 2314
StyD4I CCNGG 4 cut(s) 547, 944, 1682, 2492
StyI CCWWGG 2 cut(s) 812, 1817
TaaI ACNGT 6 cut(s) 194, 362, 1427, 1724, 2064, 2334
TaiI ACGT 2 cut(s) 379, 1569
TaqI TCGA 5 cut(s) 257, 293, 321, 345, 2271
TatI WGTACW 3 cut(s) 180, 2046, 2329
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 8 cut(s) 295, 329, 468, 1687, 1796, 2143, 2222, 2504
Tru1I TTAA 6 cut(s) 954, 1032, 1059, 1143, 1712, 1911
Tru9I TTAA 6 cut(s) 954, 1032, 1059, 1143, 1712, 1911
TscAI CASTG 4 cut(s) 241, 358, 496, 1441
TseFI GTSAC 4 cut(s) 4, 998, 1389, 1432
TseI GCWGC 6 cut(s) 422, 848, 851, 1016, 1850, 1883
Tsp45I GTSAC 4 cut(s) 4, 998, 1389, 1432
TspGWI ACGGA 3 cut(s) 74, 100, 2214
TspRI CASTG 4 cut(s) 241, 358, 496, 1441
VpaK11BI GGWCC 5 cut(s) 716, 797, 1157, 1773, 1982
XagI CCTNNNNNAGG 1 cut(s) 1874
XceI RCATGY 1 cut(s) 1373
XcmI CCANNNNNNNNNTGG 1 cut(s) 1927
XmaJI CCTAGG 1 cut(s) 812
XmiI GTMKAC 1 cut(s) 1752
XmnI GAANNNNTTC 2 cut(s) 75, 485
XspI CTAG 5 cut(s) 813, 2036, 2139, 2250, 2304
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.