Rw1G005210

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000413
Physical Location & Seq
Forward (+)
10060548 .. 10064025
3478 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005210.1

Sequence Viewer

Length: 2427 bp
ATGGAGCATCTTCGTATCCCATTACTCTCTCTGCTTGTTTTTCTCCTCAACCTCTCATCCCTTCACTTTCTCTCTTTAGCTGATGATTATTTCATCAACTGTGGCTCACATGATAATGTGAGCCTCGCTCCTGGCCAGAATTTCTCTGGGGAATCGAAGTCTGCAGGTTACTCTTTCTCCAAAAGCAAGGCTGTCAAAGACATCAACCAGTTGCCAGATATATCACCTCTATATAAGACAGCAAGAAGTTTCAATAAACCATTCTACTACCAGTTCAGCATCACTGAAGATGGTACTTATCTGGTACGCTTACATTTCTCAGCTTTCTCCACCTCCTCCTCCTCAAATAATCTCTCCACGGCTGTTTTTGGTGTTTTGGATTCTAAGAATTTCACACTGTTGAACAATTTCACCGCCAAGAACACTACCTACTCCCCTGTGATGAAGGAATTCTTTCTCCGAATTGATATCACCGACTCATTTAGACTATATTTTACTCCTCAACCATCATCTTTTGCATTTGTAAATGGCATAGAATTATTCCTTGCCCCTGCAGATTTCATCGCTGAGAACTATGCCAGTAATCTCCCTTTACACACAATTTACAGACTCAACGTTGGAGGCTCACCAGTAAATGACACAATTGGGAGAAAGTGGGAAACAGATGATAGTCATATCTCTGATCAAAACTCTGCAAAGAAAGCCACTCCTCAAAGTACACTCAATTTATATCGCTACGGAGAAATTGATGGTTTGGTTGTATCTAATGAGTCTGTAGCCCCATCTTTGGTTTACCAGACTGCTAGAGAGATGACGCATGTCACTAGTAACATAACATGGTTTTTTAGTGTGAGTAGTAAAGCTAGACACATTGTCCGGGCCCACTTCTGTGACATTGTTGGTCAGCCTGGTAACATTATATTTAACTTGTATGCAAATGGCAACTTTCGCAAGGAGATCGGCAACATTACTTCGGCTGTTCCCTTCTACTATGACTTTGTGGTGAATTACAGTGAGTCTGAACTCTTTAATATCAGCATAAGACCTAACAATGGTGCTTCTGAACAAAATGCATTTCTAAATGGTCTGGAAATATTGGAGATAGTGGAGGGATTAGCTCCAATTCCCAATGTGAAAGAGTCCAAGAAGAATGTTGTGGCTCCTGTGGTTGGTTCAGTTCTTGGAGGCCTGTCACTCATCTGCGTTTTAACAGTTGGATTTGTGTTTGGTTTCAGACACAGAAAGGCAGAAAAGCATGTGGAGACTTCAGTTTGGTCACCAATGCCTGCAAATGGAGGAGGGAGTTCTCACAGCTCCACTCTCAATCTAGATTATCTTGGGTTGAAGATATCTTTCAATGAAATTCAGTCTGCAACGAACAACTTTGACACAAAGTTGGTGATAGGTAAGGGTGGCTTTGGGAATGTTTATAGAGGCACTCTTTTGAATGGCACAAAAGTGGCTGTGAAGCGAGCTTATAAGCGAGATGAGCATGGGTCAGGATCAGGCCAAGGCCTCCTAGAATTCCAAACAGAAATCATAGTGTTATCGAAAATCCGCCACCGCCATCTTGTCTCCTTAATTGGTTATTGTAATGAAAGGTCTGAAATGATACTAGTGTATGAGTTCATGGAAAAAGGGACGTTGAGAGATCATTTGTATGATTCAGACGTGCCTCGCTTGTCGTGGAATCAAAGACTTGAAATTTGTACTGGAGCAGCAAGGGGTCTTCATTATCTCCACACAAGTGCAGCTAGGGGAATCATTCACCGAGATGTCAAGTCCACCAACATATTGCTTGATGAAAACCAGGTTGCCAAAGTTGCTGACTTTGGCCTTTCGAGATCTGGAGCTCTCGATGAAACGCATGTCAGCACTAATGTTAAAGGCACTTTTGGTTACCTTGATCCTGAGTACATGATGTCTGAACAATTGACAGAAAAATCTGATGTTTACTCATTTGGTGTAGTTCTTCTTGAGGTGTTATGTGGAAGACCTGCTATTGATCCAACGCTTCCAAGAGAGCAAATGAACTTGGCTGAATGGGGAATGCTTTGCAAGAAAAAAGGGTTGCTTGAGCAGATTGTTGATTCTTCTTTGAAAAATCAGATTGATCCTAGCTCACTAAGAACTTTTGGTGAGACGGCTGAGAAGTGTTTGCAAGATGATGCTTCTGATAGGCCAACAATGGCCGATGTGCTGTGGGATTTGGAATATGCATTACAGCTTCAGAAAACAACAAAGCTTAAAGAGGCTCATGAGGACAGCACCACCATTGATGCTTCATCAGCAGCATTCAGTTTGCCAATTGTTCAGCGTTTTCCTTCACTTGGTTCGACAACAAATGGAGATGATATGAGGGACAGCGACTTGGACACAACAGAAAACAAAATTTTCTCCCAATTGAAAATTGGTGAAGCCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

808

Amino Acids

89.66

Weight (kDa)

5.93

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 30 - 112 2.2e-07 Malectin domain
Malectin_like PF12819 32 - 367 9.2e-22 Malectin-like domain
Pkinase PF00069 465 - 734 1.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 466 - 735 4.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23200
fragaria_vesca FvH4_1g16860 FvH4_1g16861
malus_domestica MD02G1018900.v1.1 MD15G1289500.v1.1 MD15G1289600.v1.1 MD15G1289700.v1.1
prunus_persica Prupe.6G227800_v2.0.a1 Prupe.6G228000_v2.0.a1 Prupe.6G228100_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228300_v2.0.a1
pyrus_communis pycom02g01680 pycom02g01690 pycom15g25310 pycom15g25330 pycom15g25340
rosa_chinensis RchiOBHm_Chr1g0324321 RchiOBHm_Chr1g0324331 RchiOBHm_Chr1g0324341 RchiOBHm_Chr1g0324401 RchiOBHm_Chr1g0324441 RchiOBHm_Chr2g0106751 RchiOBHm_Chr2g0106761 RchiOBHm_Chr2g0106771 RchiOBHm_Chr2g0106781 RchiOBHm_Chr2g0106801 RchiOBHm_Chr2g0106811 RchiOBHm_Chr2g0106821
rosa_laevigata RLG00000017539 RLG00000017540 RLG00000017541 RLG00000030200 RLG00000030201
rosa_multiflora Rmu_sc0000751.1_g000001 Rmu_sc0000751.1_g000002 Rmu_sc0000751.1_g000003 Rmu_sc0000751.1_g000005 Rmu_sc0000751.1_g000007 Rmu_sc0000751.1_g000008 Rmu_sc0000751.1_g000018 Rmu_sc0000751.1_g000019 Rmu_sc0000751.1_g000026 Rmu_sc0000751.1_g000027 Rmu_sc0000751.1_g000028 Rmu_sc0000751.1_g000029 Rmu_sc0003410.1_g000012 Rmu_sc0004000.1_g000023 Rmu_sc0004000.1_g000024 Rmu_sc0004082.1_g000015 Rmu_sc0004082.1_g000017 Rmu_sc0026507.1_g000001 Rmu_sc0031196.1_g000001 Rmu_sc0039591.1_g000001
rosa_roxburghii Rroxscaffold_159G00432710 Rroxscaffold_159G00432780 Rroxscaffold_159G00432840 Rroxscaffold_159G00433010 Rroxscaffold_2G00098070 Rroxscaffold_2G00136770 Rroxscaffold_4G00325540 Rroxscaffold_4G00325630 Rroxscaffold_4G00325680
rosa_rugosa Rorug01G0045700 Rorug02G0138500 Rorug02G0138600 Rorug02G0138700 Rorug02G0138800 Rorug02G0138900
rosa_samantha Rh1AG061400 Rh1BG052300 Rh1CG063400 Rh1CG063700 Rh1DG067800 Rh2AG189400 Rh2AG189800 Rh2AG190000 Rh2AG190100 Rh2BG201100 Rh2BG201300 Rh2CG194500 Rh2CG194600 Rh2CG194700 Rh2DG195700 Rh2DG195900 Rh2DG196300 Rh2DG196400 Rh2DG196500 Rh2DG196600
rosa_wichuraiana Rw1G004230 Rw1G005210 Rw2G014900 Rw2G014910 Rw2G014930 Rw2G014940 Rw2G014950 Rw2G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1479
Acc36I ACCTGC 2 cut(s) 155, 2005
AciI CCGC 3 cut(s) 414, 1558, 1564
AclI AACGTT 1 cut(s) 615
AclWI GGATC 4 cut(s) 1510, 1901, 2000, 2108
AcoI YGGCCR 2 cut(s) 133, 2190
AcsI RAATTY 7 cut(s) 139, 388, 449, 1362, 1523, 1704, 2391
AcuI CTGAAG 3 cut(s) 306, 1251, 2213
AfaI GTAC 5 cut(s) 295, 306, 718, 1711, 1916
AfiI CCNNNNNNNGG 5 cut(s) 787, 1052, 1169, 1292, 2330
AgsI TTSAA 8 cut(s) 253, 403, 1345, 1357, 1447, 1703, 2101, 2407
AhdI GACNNNNNGTC 1 cut(s) 872
AhlI ACTAGT 2 cut(s) 824, 1615
AjiI CACGTC 1 cut(s) 1672
AjnI CCWGG 3 cut(s) 130, 907, 1809
AleI CACNNNNGTG 3 cut(s) 888, 1457, 1746
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 3 cut(s) 1256, 1579, 2135
AlwI GGATC 4 cut(s) 1510, 1901, 2000, 2108
AoxI GGCC 8 cut(s) 133, 879, 1186, 1507, 1513, 1834, 2180, 2190
ApaI GGGCCC 1 cut(s) 883
ApeKI GCWGC 3 cut(s) 1718, 1751, 2291
ApoI RAATTY 7 cut(s) 139, 388, 449, 1362, 1523, 1704, 2391
ArsI GACNNNNNNTTYG 2 cut(s) 1689, 1721
Asp700I GAANNNNTTC 3 cut(s) 407, 449, 453
AspS9I GGNCC 2 cut(s) 879, 880
AsuC2I CCSGG 1 cut(s) 878
BaeGI GKGCMC 1 cut(s) 883
BaeI ACNNNNGTAYC 2 cut(s) 1604, 1637
BalI TGGCCA 1 cut(s) 135
BanII GRGCYC 2 cut(s) 883, 1855
BbsI GAAGAC 2 cut(s) 1721, 1999
Bbv12I GWGCWC 1 cut(s) 1855
BbvI GCAGC 3 cut(s) 1730, 1763, 2303
BccI CCATC 5 cut(s) 284, 514, 743, 790, 1575
BceAI ACGGC 2 cut(s) 375, 2160
BcgI CGANNNNNNTGC 2 cut(s) 940, 974
BciT130I CCWGG 3 cut(s) 132, 909, 1811
BciVI GTATCC 1 cut(s) 26
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 1 cut(s) 878
BcoDI GTCTC 3 cut(s) 1256, 1579, 2135
BcuI ACTAGT 2 cut(s) 824, 1615
BfaI CTAG 8 cut(s) 804, 825, 864, 1328, 1520, 1616, 1755, 2118
BfmI CTRYAG 3 cut(s) 162, 552, 774
BfuAI ACCTGC 2 cut(s) 155, 2005
BfuI GTATCC 1 cut(s) 26
BglII AGATCT 1 cut(s) 1844
BisI GCNGC 3 cut(s) 1719, 1752, 2292
BlsI GCNGC 3 cut(s) 1720, 1753, 2293
Bme1390I CCNGG 4 cut(s) 132, 878, 909, 1811
BmeRI GACNNNNNGTC 1 cut(s) 872
BmgBI CACGTC 1 cut(s) 1672
BmgT120I GGNCC 2 cut(s) 879, 880
BmiI GGNNCC 2 cut(s) 881, 1161
BmrFI CCNGG 4 cut(s) 132, 878, 909, 1811
BmsI GCATC 4 cut(s) 16, 288, 2158, 2269
BoxI GACNNNNGTC 1 cut(s) 818
BpiI GAAGAC 2 cut(s) 1721, 1999
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpmI CTGGAG 2 cut(s) 1734, 1869
BpuEI CTTGAG 2 cut(s) 1997, 2096
BpuMI CCSGG 1 cut(s) 878
BsaJI CCNNGG 2 cut(s) 357, 1510
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 5 cut(s) 787, 1052, 1169, 1292, 2330
Bse1I ACTGG 5 cut(s) 208, 271, 579, 629, 1717
BseBI CCWGG 3 cut(s) 132, 909, 1811
BseDI CCNNGG 2 cut(s) 357, 1510
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 5 cut(s) 787, 1052, 1169, 1292, 2330
BseMII CTCAG 4 cut(s) 333, 558, 1902, 2139
BseNI ACTGG 5 cut(s) 208, 271, 579, 629, 1717
BseRI GAGGAG 7 cut(s) 35, 325, 328, 331, 489, 699, 1311
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 3 cut(s) 1730, 1763, 2303
BsgI GTGCAG 1 cut(s) 1770
BshFI GGCC 8 cut(s) 135, 881, 1188, 1509, 1515, 1836, 2182, 2192
BsiHKAI GWGCWC 1 cut(s) 1855
BsiSI CCGG 1 cut(s) 877
BslFI GGGAC 2 cut(s) 1654, 2375
BslI CCNNNNNNNGG 5 cut(s) 787, 1052, 1169, 1292, 2330
BsmAI GTCTC 3 cut(s) 1256, 1579, 2135
BsmBI CGTCTC 1 cut(s) 2135
BsmFI GGGAC 2 cut(s) 1654, 2375
BsmI GAATGC 2 cut(s) 2055, 2294
BsnI GGCC 8 cut(s) 135, 881, 1188, 1509, 1515, 1836, 2182, 2192
Bsp120I GGGCCC 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 883, 1855
Bsp143I GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
BspACI CCGC 3 cut(s) 414, 1558, 1564
BspANI GGCC 8 cut(s) 135, 881, 1188, 1509, 1515, 1836, 2182, 2192
BspCNI CTCAG 4 cut(s) 332, 559, 1903, 2140
BspHI TCATGA 1 cut(s) 2257
BspLI GGNNCC 2 cut(s) 881, 1161
BspMAI CTGCAG 2 cut(s) 166, 556
BspMI ACCTGC 2 cut(s) 155, 2005
BspPI GGATC 4 cut(s) 1510, 1901, 2000, 2108
BsrI ACTGG 5 cut(s) 208, 271, 579, 629, 1717
BssECI CCNNGG 2 cut(s) 357, 1510
BssMI GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
BssT1I CCWWGG 1 cut(s) 1510
Bst2UI CCWGG 3 cut(s) 132, 909, 1811
Bst4CI ACNGT 4 cut(s) 101, 399, 1013, 1213
BstC8I GCNNGC 2 cut(s) 1287, 1473
BstDEI CTNAG 6 cut(s) 319, 384, 567, 1911, 2126, 2148
BstDSI CCRYGG 1 cut(s) 357
BstEII GGTNACC 2 cut(s) 1275, 1898
BstF5I GGATG 1 cut(s) 56
BstKTI GATC 8 cut(s) 685, 960, 1505, 1654, 1847, 1909, 2008, 2116
BstMAI GTCTC 3 cut(s) 1256, 1579, 2135
BstMBI GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
BstMWI GCNNNNNNNGC 5 cut(s) 701, 948, 1489, 1823, 2288
BstNI CCWGG 3 cut(s) 132, 909, 1811
BstNSI RCATGY 3 cut(s) 821, 1259, 1871
BstPAI GACNNNNGTC 1 cut(s) 818
BstPI GGTNACC 2 cut(s) 1275, 1898
BstSCI CCNGG 4 cut(s) 130, 876, 907, 1809
BstSFI CTRYAG 3 cut(s) 162, 552, 774
BstSLI GKGCMC 1 cut(s) 883
BstV1I GCAGC 3 cut(s) 1730, 1763, 2303
BstV2I GAAGAC 2 cut(s) 1721, 1999
BstX2I RGATCY 1 cut(s) 1844
BstYI RGATCY 1 cut(s) 1844
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 8 cut(s) 135, 881, 1188, 1509, 1515, 1836, 2182, 2192
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 547
BtrI CACGTC 1 cut(s) 1672
BtsCI GGATG 1 cut(s) 56
BtsIMutI CAGTG 3 cut(s) 282, 395, 1018
BveI ACCTGC 2 cut(s) 155, 2005
Cac8I GCNNGC 2 cut(s) 1287, 1473
CciI TCATGA 1 cut(s) 2257
Cfr13I GGNCC 2 cut(s) 879, 880
CseI GACGC 1 cut(s) 823
CsiI ACCWGGT 1 cut(s) 1809
Csp6I GTAC 5 cut(s) 294, 305, 717, 1710, 1915
CviAII CATG 9 cut(s) 110, 818, 837, 1256, 1493, 1630, 1868, 1918, 2258
CviQI GTAC 5 cut(s) 294, 305, 717, 1710, 1915
DdeI CTNAG 6 cut(s) 319, 384, 567, 1911, 2126, 2148
DpnI GATC 8 cut(s) 684, 959, 1504, 1653, 1846, 1908, 2007, 2115
DpnII GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
DriI GACNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 2 cut(s) 133, 2190
Eam1105I GACNNNNNGTC 1 cut(s) 872
EciI GGCGGA 1 cut(s) 1547
Ecl136II GAGCTC 1 cut(s) 1853
Eco130I CCWWGG 1 cut(s) 1510
Eco147I AGGCCT 2 cut(s) 1188, 1515
Eco24I GRGCYC 2 cut(s) 883, 1855
Eco32I GATATC 2 cut(s) 469, 1350
Eco53kI GAGCTC 1 cut(s) 1853
Eco57I CTGAAG 3 cut(s) 306, 1251, 2213
Eco91I GGTNACC 2 cut(s) 1275, 1898
EcoICRI GAGCTC 1 cut(s) 1853
EcoO65I GGTNACC 2 cut(s) 1275, 1898
EcoRI GAATTC 2 cut(s) 449, 1523
EcoRII CCWGG 3 cut(s) 130, 907, 1809
EcoRV GATATC 2 cut(s) 469, 1350
EcoT14I CCWWGG 1 cut(s) 1510
EcoT22I ATGCAT 2 cut(s) 1075, 2221
EcoT38I GRGCYC 2 cut(s) 883, 1855
ErhI CCWWGG 1 cut(s) 1510
Esp3I CGTCTC 1 cut(s) 2135
FaeI CATG 9 cut(s) 113, 821, 840, 1259, 1496, 1633, 1871, 1921, 2261
FalI AAGNNNNNCTT 6 cut(s) 437, 469, 1400, 1432, 2058, 2090
FaqI GGGAC 2 cut(s) 1654, 2375
FatI CATG 9 cut(s) 109, 817, 836, 1255, 1492, 1629, 1867, 1917, 2257
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 3 cut(s) 1719, 1752, 2292
FokI GGATG 1 cut(s) 43
FriOI GRGCYC 2 cut(s) 883, 1855
Fsp4HI GCNGC 3 cut(s) 1719, 1752, 2292
FspBI CTAG 8 cut(s) 804, 825, 864, 1328, 1520, 1616, 1755, 2118
GluI GCNGC 3 cut(s) 1719, 1752, 2292
GsuI CTGGAG 2 cut(s) 1734, 1869
HaeIII GGCC 8 cut(s) 135, 881, 1188, 1509, 1515, 1836, 2182, 2192
HapII CCGG 1 cut(s) 877
HgaI GACGC 1 cut(s) 823
Hin1II CATG 9 cut(s) 113, 821, 840, 1259, 1496, 1633, 1871, 1921, 2261
HindIII AAGCTT 1 cut(s) 2243
HpaII CCGG 1 cut(s) 877
Hpy166II GTNNAC 4 cut(s) 719, 793, 1785, 1954
Hpy188III TCNNGA 9 cut(s) 1088, 1328, 1500, 1842, 1848, 1856, 1910, 1976, 2258
Hpy8I GTNNAC 4 cut(s) 719, 793, 1785, 1954
HpyAV CCTTC 4 cut(s) 71, 439, 994, 2334
HpyCH4III ACNGT 4 cut(s) 101, 399, 1013, 1213
HpyCH4IV ACGT 3 cut(s) 615, 1643, 1671
HpyF10VI GCNNNNNNNGC 5 cut(s) 701, 948, 1489, 1823, 2288
HpyF3I CTNAG 6 cut(s) 319, 384, 567, 1911, 2126, 2148
HpySE526I ACGT 3 cut(s) 615, 1643, 1671
Hsp92II CATG 9 cut(s) 113, 821, 840, 1259, 1496, 1633, 1871, 1921, 2261
Ksp22I TGATCA 1 cut(s) 682
Kzo9I GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
LmnI GCTCC 7 cut(s) 4, 133, 1123, 1165, 1319, 1715, 1850
Lsp1109I GCAGC 3 cut(s) 1730, 1763, 2303
LweI GCATC 4 cut(s) 16, 288, 2158, 2269
MabI ACCWGGT 1 cut(s) 1809
MaeI CTAG 8 cut(s) 804, 825, 864, 1328, 1520, 1616, 1755, 2118
MaeII ACGT 3 cut(s) 615, 1643, 1671
MaeIII GTNAC 8 cut(s) 167, 820, 827, 890, 911, 1191, 1275, 1898
MalI GATC 8 cut(s) 684, 959, 1504, 1653, 1846, 1908, 2007, 2115
MboI GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
MboII GAAGA 7 cut(s) 299, 1159, 1357, 1721, 1964, 2004, 2085
MfeI CAATTG 4 cut(s) 642, 1931, 2307, 2402
MflI RGATCY 1 cut(s) 1844
MhlI GDGCHC 2 cut(s) 883, 1855
MlsI TGGCCA 1 cut(s) 135
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 5 cut(s) 470, 603, 779, 1025, 1148
MmeI TCCRAC 3 cut(s) 598, 1195, 2033
Mox20I TGGCCA 1 cut(s) 135
Mph1103I ATGCAT 2 cut(s) 1075, 2221
MroXI GAANNNNTTC 3 cut(s) 407, 449, 453
MscI TGGCCA 1 cut(s) 135
MseI TTAA 6 cut(s) 924, 1029, 1208, 1580, 1884, 2247
MslI CAYNNNNRTG 6 cut(s) 114, 888, 1457, 1659, 1746, 1773
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 1 cut(s) 877
MspR9I CCNGG 4 cut(s) 132, 878, 909, 1811
MunI CAATTG 4 cut(s) 642, 1931, 2307, 2402
Mva1269I GAATGC 2 cut(s) 2055, 2294
MvaI CCWGG 3 cut(s) 132, 909, 1811
MwoI GCNNNNNNNGC 5 cut(s) 701, 948, 1489, 1823, 2288
NciI CCSGG 1 cut(s) 878
NdeII GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
NlaIII CATG 9 cut(s) 113, 821, 840, 1259, 1496, 1633, 1871, 1921, 2261
NlaIV GGNNCC 2 cut(s) 881, 1161
NmuCI GTSAC 4 cut(s) 820, 890, 1191, 1275
NsiI ATGCAT 2 cut(s) 1075, 2221
NspI RCATGY 3 cut(s) 821, 1259, 1871
OliI CACNNNNGTG 3 cut(s) 888, 1457, 1746
PagI TCATGA 1 cut(s) 2257
PceI AGGCCT 2 cut(s) 1188, 1515
PctI GAATGC 2 cut(s) 2055, 2294
PdmI GAANNNNTTC 3 cut(s) 407, 449, 453
PfeI GAWTC 6 cut(s) 152, 380, 1664, 1690, 1761, 2090
PkrI GCNGC 3 cut(s) 1720, 1753, 2293
PleI GAGTC 5 cut(s) 470, 603, 778, 1024, 1147
PpsI GAGTC 5 cut(s) 470, 603, 778, 1024, 1147
PshAI GACNNNNGTC 1 cut(s) 818
PsiI TTATAA 1 cut(s) 1479
Psp124BI GAGCTC 1 cut(s) 1855
Psp1406I AACGTT 1 cut(s) 615
Psp6I CCWGG 3 cut(s) 130, 907, 1809
PspEI GGTNACC 2 cut(s) 1275, 1898
PspGI CCWGG 3 cut(s) 130, 907, 1809
PspN4I GGNNCC 2 cut(s) 881, 1161
PspOMI GGGCCC 1 cut(s) 879
PspPI GGNCC 2 cut(s) 879, 880
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstI CTGCAG 2 cut(s) 166, 556
PsuI RGATCY 1 cut(s) 1844
RsaI GTAC 5 cut(s) 295, 306, 718, 1711, 1916
RsaNI GTAC 5 cut(s) 294, 305, 717, 1710, 1915
RseI CAYNNNNRTG 6 cut(s) 114, 888, 1457, 1659, 1746, 1773
SacI GAGCTC 1 cut(s) 1855
SaqAI TTAA 6 cut(s) 924, 1029, 1208, 1580, 1884, 2247
SatI GCNGC 3 cut(s) 1719, 1752, 2292
Sau3AI GATC 8 cut(s) 682, 957, 1502, 1651, 1844, 1906, 2005, 2113
Sau96I GGNCC 2 cut(s) 879, 880
SchI GAGTC 5 cut(s) 470, 603, 779, 1025, 1148
ScrFI CCNGG 4 cut(s) 132, 878, 909, 1811
SduI GDGCHC 2 cut(s) 883, 1855
SexAI ACCWGGT 1 cut(s) 1809
SfaNI GCATC 4 cut(s) 16, 288, 2158, 2269
SfcI CTRYAG 3 cut(s) 162, 552, 774
SmiMI CAYNNNNRTG 6 cut(s) 114, 888, 1457, 1659, 1746, 1773
SmlI CTYRAG 2 cut(s) 1976, 2075
SmoI CTYRAG 2 cut(s) 1976, 2075
SpeI ACTAGT 2 cut(s) 824, 1615
SseBI AGGCCT 2 cut(s) 1188, 1515
SsiI CCGC 3 cut(s) 414, 1558, 1564
SspI AATATT 1 cut(s) 1095
SspMI CTAG 8 cut(s) 804, 825, 864, 1328, 1520, 1616, 1755, 2118
SstI GAGCTC 1 cut(s) 1855
StuI AGGCCT 2 cut(s) 1188, 1515
StyD4I CCNGG 4 cut(s) 130, 876, 907, 1809
StyI CCWWGG 1 cut(s) 1510
TaaI ACNGT 4 cut(s) 101, 399, 1013, 1213
TaiI ACGT 3 cut(s) 618, 1646, 1674
TaqI TCGA 5 cut(s) 155, 1550, 1841, 1857, 2336
TatI WGTACW 3 cut(s) 716, 1709, 1914
TfiI GAWTC 6 cut(s) 152, 380, 1664, 1690, 1761, 2090
Tru1I TTAA 6 cut(s) 924, 1029, 1208, 1580, 1884, 2247
Tru9I TTAA 6 cut(s) 924, 1029, 1208, 1580, 1884, 2247
TscAI CASTG 3 cut(s) 289, 402, 1018
TseFI GTSAC 4 cut(s) 820, 890, 1191, 1275
TseI GCWGC 3 cut(s) 1718, 1751, 2291
Tsp45I GTSAC 4 cut(s) 820, 890, 1191, 1275
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 3 cut(s) 289, 402, 1018
XapI RAATTY 7 cut(s) 139, 388, 449, 1362, 1523, 1704, 2391
XbaI TCTAGA 1 cut(s) 1327
XceI RCATGY 3 cut(s) 821, 1259, 1871
XcmI CCANNNNNNNNNTGG 2 cut(s) 143, 2408
XmnI GAANNNNTTC 3 cut(s) 407, 449, 453
XspI CTAG 8 cut(s) 804, 825, 864, 1328, 1520, 1616, 1755, 2118
Zsp2I ATGCAT 2 cut(s) 1075, 2221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.