Rroxscaffold_159G00432710

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000159
OG0000413
Physical Location & Seq
Forward (+)
314260 .. 328498
14239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_159G00432710.1

Sequence Viewer

Length: 1308 bp
ATGGTTTTGAGCGTGGGTAGTAAAGCTAGACACATTGTCCGGGCTCACTTCTGTGACATTGTTGGTCAGCCTGGTAACATTATATTTAACTTGTATGAAAATGGAAACTTCCGCAAGGAGATCGGCGCCACTCTTCAATCTTCGGCTGTTCCCTTCTACTATGACTTTCTGGTGAATTATAGTGAGTCTAAACTCCTTAATATCAGAAAAAGATCTAAGGATGGTGCTTTTGAACAAAATGCATTTCTAAATGGTCTGGAAATATTGGAGATAGTGGGGGGATTAGCTCCAATTCCCAATGTGAAAGAGTCCAAGAAGAAAGTTGTGGCTCCTGTGGTTGGTTCAGTTCTTAGAGGCCTAAAGGCAGAAAAGCATGTGGAGACTTCAGTTTGGTCACCAATGCTTGCAAATGGAGGAGGGCGTTCTCACAGCTCTGCTCTCAATCTAGATTATCTTGGGTTGAAGATATCTTTCAATGAAATTCAGTCTGCAACGAACAACTTTGACACAAAGTTGGTGATAGGTAAGGGTGGCTTTGGGAATGTTTATAGAGGCACTCTTTTGAATGGCACAAAAGTGGCTGTGAAGCGAGCTTATAAGCGAGATGAGCATGGGTCAGGATCAGGCCAAGGCCTCCTAGAATTCGCAACAGAAATCATAGTTTTATCGAAAATCCTCCACCGCCATCTTGTCTCCTTAATTGGTTACTGTAATGAAAGGTCTGAAATGATACTAGTGTATGAGTTCATGGAAAAAGGGATGTTGAGAGATCATTTGTATGATTCAGAGGTGCCTCGCTTGTCGTGGAATCAAATACTTGAAATTTGTACTGGAGCAGCAAGGGGTCTTCATTATCTCCACACAGGTGCAGCTAGGGGAATCATTCACCGAGATGTCAAGTCCACCAACATATTGCTTGATGAAAACCGTGTTGCCAAAGTTGCTGACTTTGGCCTTTCGAGATTTGGAGCTCTCGATGAAACGCATCTCTGCACTAATGTTAAAGGCACTTTTGGTTACCTTGATACTGAGTACATGATGTCTGAACAATTGACAGAAAAATCTGATGTTTACTCATTTGGTGTAGTTCTTCTTGAGCATGGGTTTTTCCCCGATGTCTACAGTAGACTTGTGCTTGTGTGGCAGCCTTTGTGGCGGTGCCGTAATGATTTTGTAGCAGCTTTTGTAGCAAGTGCTTATGTGACGGGCAGCTTGTGTAGCGGTGCTTGTATGGCGACCTTTGTGGCGGTGCCCCATCTTGAATACCCTTCTGTCTCCACCCTTATAAACTCATTCCAGTCCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

435

Amino Acids

48.13

Weight (kDa)

8.34

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 171 - 366 1.3e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 172 - 366 3.1e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23200
fragaria_vesca FvH4_1g16860 FvH4_1g16861
malus_domestica MD02G1018900.v1.1 MD15G1289500.v1.1 MD15G1289600.v1.1 MD15G1289700.v1.1
prunus_persica Prupe.6G227800_v2.0.a1 Prupe.6G228000_v2.0.a1 Prupe.6G228100_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228300_v2.0.a1
pyrus_communis pycom02g01680 pycom02g01690 pycom15g25310 pycom15g25330 pycom15g25340
rosa_chinensis RchiOBHm_Chr1g0324321 RchiOBHm_Chr1g0324331 RchiOBHm_Chr1g0324341 RchiOBHm_Chr1g0324401 RchiOBHm_Chr1g0324441 RchiOBHm_Chr2g0106751 RchiOBHm_Chr2g0106761 RchiOBHm_Chr2g0106771 RchiOBHm_Chr2g0106781 RchiOBHm_Chr2g0106801 RchiOBHm_Chr2g0106811 RchiOBHm_Chr2g0106821
rosa_laevigata RLG00000017539 RLG00000017540 RLG00000017541 RLG00000030200 RLG00000030201
rosa_multiflora Rmu_sc0000751.1_g000001 Rmu_sc0000751.1_g000002 Rmu_sc0000751.1_g000003 Rmu_sc0000751.1_g000005 Rmu_sc0000751.1_g000007 Rmu_sc0000751.1_g000008 Rmu_sc0000751.1_g000018 Rmu_sc0000751.1_g000019 Rmu_sc0000751.1_g000026 Rmu_sc0000751.1_g000027 Rmu_sc0000751.1_g000028 Rmu_sc0000751.1_g000029 Rmu_sc0003410.1_g000012 Rmu_sc0004000.1_g000023 Rmu_sc0004000.1_g000024 Rmu_sc0004082.1_g000015 Rmu_sc0004082.1_g000017 Rmu_sc0026507.1_g000001 Rmu_sc0031196.1_g000001 Rmu_sc0039591.1_g000001
rosa_roxburghii Rroxscaffold_159G00432710 Rroxscaffold_159G00432780 Rroxscaffold_159G00432840 Rroxscaffold_159G00433010 Rroxscaffold_2G00098070 Rroxscaffold_2G00136770 Rroxscaffold_4G00325540 Rroxscaffold_4G00325630 Rroxscaffold_4G00325680
rosa_rugosa Rorug01G0045700 Rorug02G0138500 Rorug02G0138600 Rorug02G0138700 Rorug02G0138800 Rorug02G0138900
rosa_samantha Rh1AG061400 Rh1BG052300 Rh1CG063400 Rh1CG063700 Rh1DG067800 Rh2AG189400 Rh2AG189800 Rh2AG190000 Rh2AG190100 Rh2BG201100 Rh2BG201300 Rh2CG194500 Rh2CG194600 Rh2CG194700 Rh2DG195700 Rh2DG195900 Rh2DG196300 Rh2DG196400 Rh2DG196500 Rh2DG196600
rosa_wichuraiana Rw1G004230 Rw1G005210 Rw2G014900 Rw2G014910 Rw2G014930 Rw2G014940 Rw2G014950 Rw2G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 597, 1286
AccB1I GGYRCC 4 cut(s) 125, 790, 1158, 1249
AccI GTMKAC 2 cut(s) 1119, 1126
AciI CCGC 5 cut(s) 112, 682, 1156, 1221, 1247
AclWI GGATC 1 cut(s) 628
AcsI RAATTY 3 cut(s) 480, 641, 822
AcuI CTGAAG 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 829, 1034
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 7 cut(s) 137, 233, 463, 475, 565, 821, 1262
AhdI GACNNNNNGTC 1 cut(s) 35
AhlI ACTAGT 1 cut(s) 733
AjnI CCWGG 1 cut(s) 70
AleI CACNNNNGTG 3 cut(s) 51, 575, 864
AluBI AGCT 8 cut(s) 26, 287, 432, 593, 872, 971, 1181, 1212
AluI AGCT 8 cut(s) 26, 287, 432, 593, 872, 971, 1181, 1212
Alw21I GWGCWC 1 cut(s) 973
Alw26I GTCTC 3 cut(s) 374, 697, 1279
AlwI GGATC 1 cut(s) 628
AoxI GGCC 4 cut(s) 355, 625, 631, 952
ApeKI GCWGC 5 cut(s) 836, 869, 1144, 1178, 1209
ApoI RAATTY 3 cut(s) 480, 641, 822
AspLEI GCGC 1 cut(s) 128
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 4 cut(s) 184, 387, 529, 878
BaeGI GKGCMC 1 cut(s) 1254
BaeI ACNNNNGTAYC 2 cut(s) 722, 755
BanI GGYRCC 4 cut(s) 125, 790, 1158, 1249
BanII GRGCYC 2 cut(s) 46, 973
BbsI GAAGAC 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 973
BbvI GCAGC 5 cut(s) 848, 881, 1156, 1190, 1221
BccI CCATC 3 cut(s) 215, 693, 1263
BceAI ACGGC 1 cut(s) 1146
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 1 cut(s) 72
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 3 cut(s) 374, 697, 1279
BcuI ACTAGT 1 cut(s) 733
BfaI CTAG 5 cut(s) 27, 446, 638, 734, 873
BfmI CTRYAG 1 cut(s) 1120
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 1153
BglII AGATCT 1 cut(s) 212
BisI GCNGC 5 cut(s) 837, 870, 1145, 1179, 1210
BlsI GCNGC 5 cut(s) 838, 871, 1146, 1180, 1211
Bme1390I CCNGG 2 cut(s) 41, 72
BmeRI GACNNNNNGTC 1 cut(s) 35
BmiI GGNNCC 5 cut(s) 127, 330, 792, 1160, 1251
BmrFI CCNGG 2 cut(s) 41, 72
BmsI GCATC 1 cut(s) 994
BpiI GAAGAC 1 cut(s) 839
BpmI CTGGAG 1 cut(s) 852
BpuEI CTTGAG 1 cut(s) 1115
BpuMI CCSGG 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 628
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 835, 1297
BseBI CCWGG 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 628
BseGI GGATG 2 cut(s) 226, 765
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 1020
BseNI ACTGG 2 cut(s) 835, 1297
BseRI GAGGAG 1 cut(s) 429
BseSI GKGCMC 1 cut(s) 1254
BseXI GCAGC 5 cut(s) 848, 881, 1156, 1190, 1221
BsgI GTGCAG 2 cut(s) 888, 976
BshFI GGCC 4 cut(s) 357, 627, 633, 954
BshNI GGYRCC 4 cut(s) 125, 790, 1158, 1249
BsiHKAI GWGCWC 1 cut(s) 973
BsiSI CCGG 1 cut(s) 40
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 3 cut(s) 374, 697, 1279
BsnI GGCC 4 cut(s) 357, 627, 633, 954
Bsp1286I GDGCHC 3 cut(s) 46, 973, 1254
Bsp143I GATC 4 cut(s) 120, 212, 620, 769
BspACI CCGC 5 cut(s) 112, 682, 1156, 1221, 1247
BspANI GGCC 4 cut(s) 357, 627, 633, 954
BspCNI CTCAG 1 cut(s) 1021
BspLI GGNNCC 5 cut(s) 127, 330, 792, 1160, 1251
BspPI GGATC 1 cut(s) 628
BspT107I GGYRCC 4 cut(s) 125, 790, 1158, 1249
BsrI ACTGG 2 cut(s) 835, 1297
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 4 cut(s) 120, 212, 620, 769
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 628
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 3 cut(s) 710, 929, 1124
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 405, 591
BstDEI CTNAG 3 cut(s) 216, 350, 1029
BstEII GGTNACC 2 cut(s) 393, 1016
BstF5I GGATG 2 cut(s) 226, 765
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 4 cut(s) 123, 215, 623, 772
BstMAI GTCTC 3 cut(s) 374, 697, 1279
BstMBI GATC 4 cut(s) 120, 212, 620, 769
BstMWI GCNNNNNNNGC 7 cut(s) 607, 941, 1141, 1153, 1187, 1218, 1232
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 1 cut(s) 377
BstPI GGTNACC 2 cut(s) 393, 1016
BstSCI CCNGG 2 cut(s) 39, 70
BstSFI CTRYAG 1 cut(s) 1120
BstSLI GKGCMC 1 cut(s) 1254
BstV1I GCAGC 5 cut(s) 848, 881, 1156, 1190, 1221
BstV2I GAAGAC 1 cut(s) 839
BstX2I RGATCY 1 cut(s) 212
BstYI RGATCY 1 cut(s) 212
BsuRI GGCC 4 cut(s) 357, 627, 633, 954
BtsCI GGATG 2 cut(s) 226, 765
Cac8I GCNNGC 2 cut(s) 405, 591
CfoI GCGC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 828, 1033
CviAII CATG 5 cut(s) 374, 611, 748, 1036, 1100
CviQI GTAC 2 cut(s) 828, 1033
DdeI CTNAG 3 cut(s) 216, 350, 1029
DinI GGCGCC 1 cut(s) 127
DpnI GATC 4 cut(s) 122, 214, 622, 771
DpnII GATC 4 cut(s) 120, 212, 620, 769
DriI GACNNNNNGTC 1 cut(s) 35
Eam1104I CTCTTC 1 cut(s) 138
Eam1105I GACNNNNNGTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 971
Eco130I CCWWGG 1 cut(s) 628
Eco147I AGGCCT 2 cut(s) 357, 633
Eco24I GRGCYC 2 cut(s) 46, 973
Eco32I GATATC 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 971
Eco57I CTGAAG 1 cut(s) 369
Eco91I GGTNACC 2 cut(s) 393, 1016
EcoICRI GAGCTC 1 cut(s) 971
EcoO65I GGTNACC 2 cut(s) 393, 1016
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 70
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 628
EcoT22I ATGCAT 1 cut(s) 244
EcoT38I GRGCYC 2 cut(s) 46, 973
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 628
FaeI CATG 5 cut(s) 377, 614, 751, 1039, 1103
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FatI CATG 5 cut(s) 373, 610, 747, 1035, 1099
FblI GTMKAC 2 cut(s) 1119, 1126
Fnu4HI GCNGC 5 cut(s) 837, 870, 1145, 1179, 1210
FokI GGATG 2 cut(s) 233, 772
FriOI GRGCYC 2 cut(s) 46, 973
Fsp4HI GCNGC 5 cut(s) 837, 870, 1145, 1179, 1210
FspBI CTAG 5 cut(s) 27, 446, 638, 734, 873
GlaI GCGC 1 cut(s) 127
GluI GCNGC 5 cut(s) 837, 870, 1145, 1179, 1210
GsuI CTGGAG 1 cut(s) 852
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 4 cut(s) 357, 627, 633, 954
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 5 cut(s) 377, 614, 751, 1039, 1103
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HinfI GANTC 5 cut(s) 185, 308, 782, 808, 879
HpaII CCGG 1 cut(s) 40
HphI GGTGA 4 cut(s) 184, 387, 529, 878
Hpy166II GTNNAC 4 cut(s) 903, 1072, 1120, 1127
Hpy188I TCNGA 5 cut(s) 206, 724, 787, 1045, 1066
Hpy188III TCNNGA 7 cut(s) 257, 446, 618, 960, 974, 1094, 1259
Hpy8I GTNNAC 4 cut(s) 903, 1072, 1120, 1127
HpyAV CCTTC 2 cut(s) 163, 1278
HpyCH4III ACNGT 3 cut(s) 710, 929, 1124
HpyCH4V TGCA 5 cut(s) 242, 407, 491, 869, 993
HpyF10VI GCNNNNNNNGC 7 cut(s) 607, 941, 1141, 1153, 1187, 1218, 1232
HpyF3I CTNAG 3 cut(s) 216, 350, 1029
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 5 cut(s) 377, 614, 751, 1039, 1103
HspAI GCGC 1 cut(s) 126
KasI GGCGCC 1 cut(s) 125
Kzo9I GATC 4 cut(s) 120, 212, 620, 769
LmnI GCTCC 4 cut(s) 292, 334, 833, 968
Lsp1109I GCAGC 5 cut(s) 848, 881, 1156, 1190, 1221
LweI GCATC 1 cut(s) 994
MaeI CTAG 5 cut(s) 27, 446, 638, 734, 873
MaeIII GTNAC 6 cut(s) 53, 74, 393, 704, 1016, 1201
MalI GATC 4 cut(s) 122, 214, 622, 771
MboI GATC 4 cut(s) 120, 212, 620, 769
MboII GAAGA 6 cut(s) 125, 132, 328, 475, 839, 1082
MfeI CAATTG 1 cut(s) 1049
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 3 cut(s) 46, 973, 1254
MluCI AATT 7 cut(s) 175, 291, 480, 641, 699, 822, 1049
Mly113I GGCGCC 1 cut(s) 126
MlyI GAGTC 2 cut(s) 194, 317
MnlI CCTC 8 cut(s) 347, 407, 410, 545, 644, 686, 781, 804
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 4 cut(s) 87, 198, 698, 1002
MslI CAYNNNNRTG 5 cut(s) 51, 575, 777, 864, 891
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 41, 72
MunI CAATTG 1 cut(s) 1049
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 7 cut(s) 607, 941, 1141, 1153, 1187, 1218, 1232
NarI GGCGCC 1 cut(s) 126
NciI CCSGG 1 cut(s) 41
NdeII GATC 4 cut(s) 120, 212, 620, 769
NlaIII CATG 5 cut(s) 377, 614, 751, 1039, 1103
NlaIV GGNNCC 5 cut(s) 127, 330, 792, 1160, 1251
NmuCI GTSAC 3 cut(s) 53, 393, 1201
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 3 cut(s) 51, 575, 864
PceI AGGCCT 2 cut(s) 357, 633
PfeI GAWTC 3 cut(s) 782, 808, 879
PkrI GCNGC 5 cut(s) 838, 871, 1146, 1180, 1211
PleI GAGTC 2 cut(s) 193, 316
PluTI GGCGCC 1 cut(s) 129
PpsI GAGTC 2 cut(s) 193, 316
PsiI TTATAA 2 cut(s) 597, 1286
Psp124BI GAGCTC 1 cut(s) 973
Psp6I CCWGG 1 cut(s) 70
PspEI GGTNACC 2 cut(s) 393, 1016
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 5 cut(s) 127, 330, 792, 1160, 1251
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 2 cut(s) 829, 1034
RsaNI GTAC 2 cut(s) 828, 1033
RseI CAYNNNNRTG 5 cut(s) 51, 575, 777, 864, 891
SacI GAGCTC 1 cut(s) 973
SaqAI TTAA 4 cut(s) 87, 198, 698, 1002
SatI GCNGC 5 cut(s) 837, 870, 1145, 1179, 1210
Sau3AI GATC 4 cut(s) 120, 212, 620, 769
SchI GAGTC 2 cut(s) 194, 317
ScrFI CCNGG 2 cut(s) 41, 72
SduI GDGCHC 3 cut(s) 46, 973, 1254
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 1120
SfoI GGCGCC 1 cut(s) 127
SmiMI CAYNNNNRTG 5 cut(s) 51, 575, 777, 864, 891
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
SpeI ACTAGT 1 cut(s) 733
Sse9I AATT 7 cut(s) 175, 291, 480, 641, 699, 822, 1049
SseBI AGGCCT 2 cut(s) 357, 633
SsiI CCGC 5 cut(s) 112, 682, 1156, 1221, 1247
SspDI GGCGCC 1 cut(s) 125
SspI AATATT 1 cut(s) 264
SspMI CTAG 5 cut(s) 27, 446, 638, 734, 873
SstI GAGCTC 1 cut(s) 973
StuI AGGCCT 2 cut(s) 357, 633
StyD4I CCNGG 2 cut(s) 39, 70
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 3 cut(s) 710, 929, 1124
TaqI TCGA 3 cut(s) 668, 959, 975
TasI AATT 7 cut(s) 175, 291, 480, 641, 699, 822, 1049
TatI WGTACW 2 cut(s) 827, 1032
TfiI GAWTC 3 cut(s) 782, 808, 879
Tru1I TTAA 4 cut(s) 87, 198, 698, 1002
Tru9I TTAA 4 cut(s) 87, 198, 698, 1002
TseFI GTSAC 3 cut(s) 53, 393, 1201
TseI GCWGC 5 cut(s) 836, 869, 1144, 1178, 1209
Tsp45I GTSAC 3 cut(s) 53, 393, 1201
TspDTI ATGAA 7 cut(s) 111, 492, 729, 736, 839, 936, 993
TspGWI ACGGA 1 cut(s) 1291
XapI RAATTY 3 cut(s) 480, 641, 822
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 1119, 1126
XspI CTAG 5 cut(s) 27, 446, 638, 734, 873
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.