Rw1G004230

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000413
Physical Location & Seq
Reverse (-)
7895643 .. 7898057
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004230.1

Sequence Viewer

Length: 2415 bp
ATGGAACATCTTCGTATCCCATTACTCTCTCTGATTGTTTTTCTCCTCAATCTCTCATCCCTTCACTTTCTCTCTTTAGCTGATGATTATTTCATCAACTGTGGTTCACATGATAATGTGCGCCTCACTCCTGGCCCGAATTTCTCTGGGGAATCGAAGTCCGGTTTCACTTTCTCCAAAAGCAAGGCTGTCAAAGACATCAACCAGTTGCTAGATATATCACCTCTATATAAGACAGCAAGAGGTTTCAATAAACCATTCTACTACCAGTTCAACATCACTGAAGATGGTATTTATCTGGTACGCTTGCATTTCTCAGCTTTCTCCTCCTCCTCAAATAATCTCTCCACGGCTGTTTTTGGTGTTTTGGATTCTAAGTATTTCACACTGTTGAACAATTTCACTGCCAAGAACACTACCAACTCCCCTGTGATAAAGGAATTCTTTCTCCGAATTGATCTTACAGACTCATTTAGACTATATTTTACTCCTCAACCATCATCTTTTGCATTTGTAAATGCCATAGAGTTATTCCTTGCCCCTATAGATTTCATCGCTGAGAACTATATCAGTAATCTCCCTTTACACACAATTTACAGACTCAACGTTGGAGGCTCACCAGTAAATGACAAAATAGGGAGAAACTGGGAAACAGATGATAGTCATATTTCTGATCAAAACTCTGCAAAGAAAGCCCCTTCTCAAAATTTACTCAATTTAAATTACTTTGGACAAATTTATGGTTTCGTTGCATCTAATGAGTCTGTAGCCCCACGTTTGGTTTACCAGACTGCTAGAGAGATGACGAGTGTCACTAGTAACATAACATGGTTTTTTAGTGTGAGTAGTAAAGCTAGACACATTGTCCGGGCTCACTTCTGTGACATTGTTGGTCAGCCTGGTAACATTATATTTAACTTGTATGCAAATGGCAACTTTCGCAAGGAGATCGGCAACATTCTTTCGGCTGTTCCCTTCTACTATGACTTTGTGGTGAATTATAGTGAGTCTGAACTCTTTAATATCAGCATAGGACCTAAGAATGGTGCTTCTGAACAAAATGCATTTCTAAATGGTCTGGAAATATTGGAGATAGTGGAGGGATTAGCTCCAATTCCCAATGTGAAAGATTCTAAGAAGAATGTTGTGGCTCCTGTGGTTGGTTCAGTTCTTGGAGGTCTGTCACTCATCTGCGTTTTAATAGTTGGATTTGTGTTCGGTTTTAGCCACAGAAAGGCAGAAAAGCCTGTGGAGACTTCAGTTTGGTCACCAATGCCTGCAAATGGAGGAGGGAGTTCTCACAGCTCCCCTCTCAATCTAAATTATCTCGGGTTGAAGATATCTTTCAATGAAATTCAGTCTGCAACGAACAACTTTGACACAAAGTTGGTGATAGGTAAGGGTGGCTTTGGGAATGTTTATAGAGGCACTCTTTTCAATGGCACAAAAGTGGCTGTGAAGCGAGCTTACAAGCAAGATGAGCATGGGTCAGGATCAGCCCAAGGCCTCCTAGAATTCGAAACAGAAATCATAGTGTTATCGAAAATCCGCTACCGCCATCTTGTCTCCTTAATTGGTTACTGTAATGAAAGGTCTGAAATGATACTAGTGTATGAGTTCATGGAAAAAGGGACGTTGAGAGATCATTTGTATGATTCAAACGTGCCTCGCTTGACTTGGAAGCAAAGACTTGAAATTTGTACTGGAGCAACAAGGGGTCTTCATTATCTCCACACAGGTGCAGCTGGGGGAATCATTCACCGAGATGTCAAGTCCACCAACATATTGCTTGATGAAAACCATGTTGCCAAAGTTGCTGACTTTGGCCTTTCGAGATCTAGAGCTCTTGATGAAACGCATGTCAGCACTAATGTTAAAGGCACTTTTGGTTACCTTGATCCTGAGTACATGATGTCTGAACAATTGACAGAAAAATCTGATGTTTACTCATTTGGTGTAGTTCTTCTTGAGGTGTTATGTGGAAGACCTGCTATTGATCCAACGCTTCCAAGAGAGCAAATGAACTTGGCTGAATGGGGAATGCTTTGCACGAAAAAAGGGTTGCTTGAACAGATTGTTGATTCTTCATTGAAAAATCAGATTGATCCTAGCTCACTCAGAATGTTTGCTGAGACGGCTGAGAAGTGTTTGCAAGAAGATGCTTCTGATAGGCCAACAATGGCCGATGTGCTGTGGGATTTGGAATATGCATTACAGCTTCAGAAAACAACAAAGCTTAAAGAGGCTCATGAGAACAGCACCACCATTGATGCTTCATCAGCAGCATTCAGTTTGCCAATTGTTCAGCGTTTTCCTTCACTTGGTTCGACAACAAATGGAGATGATATGAGGGACAGCGACTTGGACACAACAGGAAACAAAATTTTCTGCCAATTGAAAATTGGTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

804

Amino Acids

89.33

Weight (kDa)

6.06

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 32 - 364 3e-19 Malectin-like domain
Pkinase PF00069 462 - 731 4.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 463 - 732 1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23200
fragaria_vesca FvH4_1g16860 FvH4_1g16861
malus_domestica MD02G1018900.v1.1 MD15G1289500.v1.1 MD15G1289600.v1.1 MD15G1289700.v1.1
prunus_persica Prupe.6G227800_v2.0.a1 Prupe.6G228000_v2.0.a1 Prupe.6G228100_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228300_v2.0.a1
pyrus_communis pycom02g01680 pycom02g01690 pycom15g25310 pycom15g25330 pycom15g25340
rosa_chinensis RchiOBHm_Chr1g0324321 RchiOBHm_Chr1g0324331 RchiOBHm_Chr1g0324341 RchiOBHm_Chr1g0324401 RchiOBHm_Chr1g0324441 RchiOBHm_Chr2g0106751 RchiOBHm_Chr2g0106761 RchiOBHm_Chr2g0106771 RchiOBHm_Chr2g0106781 RchiOBHm_Chr2g0106801 RchiOBHm_Chr2g0106811 RchiOBHm_Chr2g0106821
rosa_laevigata RLG00000017539 RLG00000017540 RLG00000017541 RLG00000030200 RLG00000030201
rosa_multiflora Rmu_sc0000751.1_g000001 Rmu_sc0000751.1_g000002 Rmu_sc0000751.1_g000003 Rmu_sc0000751.1_g000005 Rmu_sc0000751.1_g000007 Rmu_sc0000751.1_g000008 Rmu_sc0000751.1_g000018 Rmu_sc0000751.1_g000019 Rmu_sc0000751.1_g000026 Rmu_sc0000751.1_g000027 Rmu_sc0000751.1_g000028 Rmu_sc0000751.1_g000029 Rmu_sc0003410.1_g000012 Rmu_sc0004000.1_g000023 Rmu_sc0004000.1_g000024 Rmu_sc0004082.1_g000015 Rmu_sc0004082.1_g000017 Rmu_sc0026507.1_g000001 Rmu_sc0031196.1_g000001 Rmu_sc0039591.1_g000001
rosa_roxburghii Rroxscaffold_159G00432710 Rroxscaffold_159G00432780 Rroxscaffold_159G00432840 Rroxscaffold_159G00433010 Rroxscaffold_2G00098070 Rroxscaffold_2G00136770 Rroxscaffold_4G00325540 Rroxscaffold_4G00325630 Rroxscaffold_4G00325680
rosa_rugosa Rorug01G0045700 Rorug02G0138500 Rorug02G0138600 Rorug02G0138700 Rorug02G0138800 Rorug02G0138900
rosa_samantha Rh1AG061400 Rh1BG052300 Rh1CG063400 Rh1CG063700 Rh1DG067800 Rh2AG189400 Rh2AG189800 Rh2AG190000 Rh2AG190100 Rh2BG201100 Rh2BG201300 Rh2CG194500 Rh2CG194600 Rh2CG194700 Rh2DG195700 Rh2DG195900 Rh2DG196300 Rh2DG196400 Rh2DG196500 Rh2DG196600
rosa_wichuraiana Rw1G004230 Rw1G005210 Rw2G014900 Rw2G014910 Rw2G014930 Rw2G014940 Rw2G014950 Rw2G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1996
AciI CCGC 2 cut(s) 1549, 1555
AclI AACGTT 1 cut(s) 606
AclWI GGATC 4 cut(s) 1501, 1892, 1991, 2099
AcoI YGGCCR 1 cut(s) 2181
AcsI RAATTY 8 cut(s) 139, 440, 706, 735, 1353, 1514, 1695, 2382
AcuI CTGAAG 3 cut(s) 303, 1242, 2204
AfaI GTAC 3 cut(s) 303, 1702, 1907
AfiI CCNNNNNNNGG 6 cut(s) 778, 1043, 1160, 1234, 1283, 2321
AhdI GACNNNNNGTC 1 cut(s) 863
AhlI ACTAGT 2 cut(s) 815, 1606
AjnI CCWGG 2 cut(s) 130, 898
AleI CACNNNNGTG 3 cut(s) 879, 1448, 1737
Alw21I GWGCWC 1 cut(s) 1846
Alw26I GTCTC 3 cut(s) 1247, 1570, 2126
AlwI GGATC 4 cut(s) 1501, 1892, 1991, 2099
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 5 cut(s) 133, 1504, 1825, 2171, 2181
ApeKI GCWGC 2 cut(s) 1742, 2282
ApoI RAATTY 8 cut(s) 139, 440, 706, 735, 1353, 1514, 1695, 2382
ArsI GACNNNNNNTTYG 2 cut(s) 1680, 1712
Asp700I GAANNNNTTC 3 cut(s) 9, 398, 444
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 134, 1034
AsuC2I CCSGG 1 cut(s) 869
AsuHPI GGTGA 6 cut(s) 213, 609, 1006, 1260, 1402, 1751
AsuII TTCGAA 1 cut(s) 1518
AvaI CYCGRG 1 cut(s) 1328
AvaII GGWCC 1 cut(s) 1034
BaeI ACNNNNGTAYC 2 cut(s) 1595, 1628
BanII GRGCYC 2 cut(s) 874, 1846
BarI GAAGNNNNNNTAC 2 cut(s) 1451, 1483
BbsI GAAGAC 2 cut(s) 1712, 1990
Bbv12I GWGCWC 1 cut(s) 1846
BbvI GCAGC 2 cut(s) 1754, 2294
BccI CCATC 3 cut(s) 281, 505, 1566
BceAI ACGGC 2 cut(s) 366, 2151
BcgI CGANNNNNNTGC 2 cut(s) 931, 965
BciT130I CCWGG 2 cut(s) 132, 900
BciVI GTATCC 1 cut(s) 26
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 869
BcoDI GTCTC 3 cut(s) 1247, 1570, 2126
BcuI ACTAGT 2 cut(s) 815, 1606
BfaI CTAG 8 cut(s) 212, 795, 816, 855, 1511, 1607, 1839, 2109
BfmI CTRYAG 2 cut(s) 543, 765
BfuAI ACCTGC 1 cut(s) 1996
BfuI GTATCC 1 cut(s) 26
BglII AGATCT 1 cut(s) 1835
BisI GCNGC 2 cut(s) 1743, 2283
BlsI GCNGC 2 cut(s) 1744, 2284
Bme1390I CCNGG 3 cut(s) 132, 869, 900
Bme18I GGWCC 1 cut(s) 1034
BmeRI GACNNNNNGTC 1 cut(s) 863
BmeT110I CYCGRG 1 cut(s) 1328
BmgT120I GGNCC 2 cut(s) 134, 1034
BmiI GGNNCC 1 cut(s) 1152
BmrFI CCNGG 3 cut(s) 132, 869, 900
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 4 cut(s) 761, 2149, 2260, 2398
BmuI ACTGGG 1 cut(s) 655
BoxI GACNNNNGTC 1 cut(s) 809
BpiI GAAGAC 2 cut(s) 1712, 1990
BpmI CTGGAG 1 cut(s) 1725
Bpu14I TTCGAA 1 cut(s) 1518
BpuEI CTTGAG 1 cut(s) 1988
BpuMI CCSGG 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 348, 1501
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 6 cut(s) 778, 1043, 1160, 1234, 1283, 2321
Bse1I ACTGG 5 cut(s) 205, 268, 620, 650, 1708
BseBI CCWGG 2 cut(s) 132, 900
BseDI CCNNGG 2 cut(s) 348, 1501
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 6 cut(s) 778, 1043, 1160, 1234, 1283, 2321
BseMII CTCAG 6 cut(s) 330, 549, 1893, 2121, 2130, 2131
BseNI ACTGG 5 cut(s) 205, 268, 620, 650, 1708
BseRI GAGGAG 6 cut(s) 35, 316, 319, 322, 480, 1302
BseXI GCAGC 2 cut(s) 1754, 2294
BseYI CCCAGC 1 cut(s) 1745
BsgI GTGCAG 1 cut(s) 1761
BshFI GGCC 5 cut(s) 135, 1506, 1827, 2173, 2183
BsiHKAI GWGCWC 1 cut(s) 1846
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 2 cut(s) 162, 868
BslFI GGGAC 2 cut(s) 1645, 2366
BslI CCNNNNNNNGG 6 cut(s) 778, 1043, 1160, 1234, 1283, 2321
BsmAI GTCTC 3 cut(s) 1247, 1570, 2126
BsmBI CGTCTC 1 cut(s) 2126
BsmFI GGGAC 2 cut(s) 1645, 2366
BsmI GAATGC 2 cut(s) 2046, 2285
BsnI GGCC 5 cut(s) 135, 1506, 1827, 2173, 2183
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 1518
Bsp1286I GDGCHC 2 cut(s) 874, 1846
Bsp143I GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
BspACI CCGC 2 cut(s) 1549, 1555
BspANI GGCC 5 cut(s) 135, 1506, 1827, 2173, 2183
BspCNI CTCAG 6 cut(s) 329, 550, 1894, 2122, 2130, 2131
BspHI TCATGA 1 cut(s) 2248
BspLI GGNNCC 1 cut(s) 1152
BspMI ACCTGC 1 cut(s) 1996
BspPI GGATC 4 cut(s) 1501, 1892, 1991, 2099
BspT104I TTCGAA 1 cut(s) 1518
BsrI ACTGG 5 cut(s) 205, 268, 620, 650, 1708
BssECI CCNNGG 2 cut(s) 348, 1501
BssMI GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
BssT1I CCWWGG 1 cut(s) 1501
Bst2UI CCWGG 2 cut(s) 132, 900
Bst4CI ACNGT 3 cut(s) 101, 390, 1583
BstBI TTCGAA 1 cut(s) 1518
BstC8I GCNNGC 3 cut(s) 308, 1278, 1464
BstDEI CTNAG 9 cut(s) 316, 375, 558, 1038, 1134, 1902, 2117, 2130, 2139
BstDSI CCRYGG 1 cut(s) 348
BstEII GGTNACC 2 cut(s) 1266, 1889
BstF5I GGATG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 9 cut(s) 460, 676, 951, 1496, 1645, 1838, 1900, 1999, 2107
BstMAI GTCTC 3 cut(s) 1247, 1570, 2126
BstMBI GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
BstMWI GCNNNNNNNGC 6 cut(s) 692, 939, 1480, 1814, 2135, 2279
BstNI CCWGG 2 cut(s) 132, 900
BstNSI RCATGY 1 cut(s) 1862
BstPAI GACNNNNGTC 1 cut(s) 809
BstPI GGTNACC 2 cut(s) 1266, 1889
BstSCI CCNGG 3 cut(s) 130, 867, 898
BstSFI CTRYAG 2 cut(s) 543, 765
BstV1I GCAGC 2 cut(s) 1754, 2294
BstV2I GAAGAC 2 cut(s) 1712, 1990
BstX2I RGATCY 1 cut(s) 1835
BstYI RGATCY 1 cut(s) 1835
BsuI GTATCC 1 cut(s) 26
BsuRI GGCC 5 cut(s) 135, 1506, 1827, 2173, 2183
BtgI CCRYGG 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 538
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 3 cut(s) 279, 386, 402
BveI ACCTGC 1 cut(s) 1996
Cac8I GCNNGC 3 cut(s) 308, 1278, 1464
CciI TCATGA 1 cut(s) 2248
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 134, 1034
Csp6I GTAC 3 cut(s) 302, 1701, 1906
CviAII CATG 8 cut(s) 110, 828, 1484, 1621, 1802, 1859, 1909, 2249
CviQI GTAC 3 cut(s) 302, 1701, 1906
DdeI CTNAG 9 cut(s) 316, 375, 558, 1038, 1134, 1902, 2117, 2130, 2139
DpnI GATC 9 cut(s) 459, 675, 950, 1495, 1644, 1837, 1899, 1998, 2106
DpnII GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
DraI TTTAAA 1 cut(s) 720
DriI GACNNNNNGTC 1 cut(s) 863
EaeI YGGCCR 1 cut(s) 2181
Eam1105I GACNNNNNGTC 1 cut(s) 863
Ecl136II GAGCTC 1 cut(s) 1844
Eco130I CCWWGG 1 cut(s) 1501
Eco147I AGGCCT 1 cut(s) 1506
Eco24I GRGCYC 2 cut(s) 874, 1846
Eco32I GATATC 1 cut(s) 1341
Eco47I GGWCC 1 cut(s) 1034
Eco53kI GAGCTC 1 cut(s) 1844
Eco57I CTGAAG 3 cut(s) 303, 1242, 2204
Eco88I CYCGRG 1 cut(s) 1328
Eco91I GGTNACC 2 cut(s) 1266, 1889
EcoICRI GAGCTC 1 cut(s) 1844
EcoO109I RGGNCCY 1 cut(s) 1034
EcoO65I GGTNACC 2 cut(s) 1266, 1889
EcoRI GAATTC 2 cut(s) 440, 1514
EcoRII CCWGG 2 cut(s) 130, 898
EcoRV GATATC 1 cut(s) 1341
EcoT14I CCWWGG 1 cut(s) 1501
EcoT22I ATGCAT 2 cut(s) 1066, 2212
EcoT38I GRGCYC 2 cut(s) 874, 1846
ErhI CCWWGG 1 cut(s) 1501
Esp3I CGTCTC 1 cut(s) 2126
FaeI CATG 8 cut(s) 113, 831, 1487, 1624, 1805, 1862, 1912, 2252
FalI AAGNNNNNCTT 6 cut(s) 428, 460, 1391, 1423, 2049, 2081
FaqI GGGAC 2 cut(s) 1645, 2366
FatI CATG 8 cut(s) 109, 827, 1483, 1620, 1801, 1858, 1908, 2248
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 1743, 2283
FokI GGATG 1 cut(s) 43
FriOI GRGCYC 2 cut(s) 874, 1846
Fsp4HI GCNGC 2 cut(s) 1743, 2283
FspBI CTAG 8 cut(s) 212, 795, 816, 855, 1511, 1607, 1839, 2109
GlaI GCGC 1 cut(s) 122
GluI GCNGC 2 cut(s) 1743, 2283
GsaI CCCAGC 1 cut(s) 1749
GsuI CTGGAG 1 cut(s) 1725
HaeIII GGCC 5 cut(s) 135, 1506, 1827, 2173, 2183
HapII CCGG 2 cut(s) 162, 868
HhaI GCGC 1 cut(s) 123
Hin1II CATG 8 cut(s) 113, 831, 1487, 1624, 1805, 1862, 1912, 2252
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 2234
HpaII CCGG 2 cut(s) 162, 868
HphI GGTGA 6 cut(s) 213, 609, 1006, 1260, 1402, 1751
Hpy166II GTNNAC 4 cut(s) 107, 784, 1776, 1945
Hpy188III TCNNGA 8 cut(s) 1079, 1491, 1833, 1839, 1847, 1901, 1967, 2249
Hpy8I GTNNAC 4 cut(s) 107, 784, 1776, 1945
HpyAV CCTTC 4 cut(s) 71, 708, 985, 2325
HpyCH4III ACNGT 3 cut(s) 101, 390, 1583
HpyCH4IV ACGT 4 cut(s) 606, 775, 1634, 1662
HpyF10VI GCNNNNNNNGC 6 cut(s) 692, 939, 1480, 1814, 2135, 2279
HpyF3I CTNAG 9 cut(s) 316, 375, 558, 1038, 1134, 1902, 2117, 2130, 2139
HpySE526I ACGT 4 cut(s) 606, 775, 1634, 1662
Hsp92II CATG 8 cut(s) 113, 831, 1487, 1624, 1805, 1862, 1912, 2252
HspAI GCGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
LmnI GCTCC 4 cut(s) 1114, 1156, 1310, 1706
Lsp1109I GCAGC 2 cut(s) 1754, 2294
LweI GCATC 4 cut(s) 761, 2149, 2260, 2398
MaeI CTAG 8 cut(s) 212, 795, 816, 855, 1511, 1607, 1839, 2109
MaeII ACGT 4 cut(s) 606, 775, 1634, 1662
MaeIII GTNAC 8 cut(s) 811, 818, 881, 902, 1182, 1266, 1577, 1889
MalI GATC 9 cut(s) 459, 675, 950, 1495, 1644, 1837, 1899, 1998, 2106
MboI GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
MboII GAAGA 8 cut(s) 296, 1150, 1348, 1712, 1955, 1995, 2076, 2168
MfeI CAATTG 3 cut(s) 1922, 2298, 2393
MflI RGATCY 1 cut(s) 1835
MhlI GDGCHC 2 cut(s) 874, 1846
MlyI GAGTC 4 cut(s) 461, 594, 770, 1016
MmeI TCCRAC 3 cut(s) 589, 1186, 2024
Mph1103I ATGCAT 2 cut(s) 1066, 2212
MroXI GAANNNNTTC 3 cut(s) 9, 398, 444
MseI TTAA 7 cut(s) 719, 915, 1020, 1199, 1571, 1875, 2238
MslI CAYNNNNRTG 6 cut(s) 114, 879, 1448, 1650, 1737, 1764
MspA1I CMGCKG 1 cut(s) 1745
MspI CCGG 2 cut(s) 162, 868
MspR9I CCNGG 3 cut(s) 132, 869, 900
MunI CAATTG 3 cut(s) 1922, 2298, 2393
Mva1269I GAATGC 2 cut(s) 2046, 2285
MvaI CCWGG 2 cut(s) 132, 900
MwoI GCNNNNNNNGC 6 cut(s) 692, 939, 1480, 1814, 2135, 2279
NciI CCSGG 1 cut(s) 869
NdeII GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
NlaIII CATG 8 cut(s) 113, 831, 1487, 1624, 1805, 1862, 1912, 2252
NlaIV GGNNCC 1 cut(s) 1152
NmuCI GTSAC 4 cut(s) 811, 881, 1182, 1266
NsiI ATGCAT 2 cut(s) 1066, 2212
NspI RCATGY 1 cut(s) 1862
NspV TTCGAA 1 cut(s) 1518
OliI CACNNNNGTG 3 cut(s) 879, 1448, 1737
PagI TCATGA 1 cut(s) 2248
PceI AGGCCT 1 cut(s) 1506
PctI GAATGC 2 cut(s) 2046, 2285
PdmI GAANNNNTTC 3 cut(s) 9, 398, 444
PfeI GAWTC 6 cut(s) 152, 371, 1130, 1655, 1752, 2081
PkrI GCNGC 2 cut(s) 1744, 2284
PleI GAGTC 4 cut(s) 461, 594, 769, 1015
PpsI GAGTC 4 cut(s) 461, 594, 769, 1015
PpuMI RGGWCCY 1 cut(s) 1034
PshAI GACNNNNGTC 1 cut(s) 809
Psp124BI GAGCTC 1 cut(s) 1846
Psp1406I AACGTT 1 cut(s) 606
Psp5II RGGWCCY 1 cut(s) 1034
Psp6I CCWGG 2 cut(s) 130, 898
PspEI GGTNACC 2 cut(s) 1266, 1889
PspFI CCCAGC 1 cut(s) 1745
PspGI CCWGG 2 cut(s) 130, 898
PspN4I GGNNCC 1 cut(s) 1152
PspPI GGNCC 2 cut(s) 134, 1034
PspPPI RGGWCCY 1 cut(s) 1034
PsuI RGATCY 1 cut(s) 1835
PvuII CAGCTG 1 cut(s) 1745
RsaI GTAC 3 cut(s) 303, 1702, 1907
RsaNI GTAC 3 cut(s) 302, 1701, 1906
RseI CAYNNNNRTG 6 cut(s) 114, 879, 1448, 1650, 1737, 1764
SacI GAGCTC 1 cut(s) 1846
SaqAI TTAA 7 cut(s) 719, 915, 1020, 1199, 1571, 1875, 2238
SatI GCNGC 2 cut(s) 1743, 2283
Sau3AI GATC 9 cut(s) 457, 673, 948, 1493, 1642, 1835, 1897, 1996, 2104
Sau96I GGNCC 2 cut(s) 134, 1034
SchI GAGTC 4 cut(s) 461, 594, 770, 1016
ScrFI CCNGG 3 cut(s) 132, 869, 900
SduI GDGCHC 2 cut(s) 874, 1846
SfaNI GCATC 4 cut(s) 761, 2149, 2260, 2398
SfcI CTRYAG 2 cut(s) 543, 765
SfuI TTCGAA 1 cut(s) 1518
SinI GGWCC 1 cut(s) 1034
SmiI ATTTAAAT 1 cut(s) 720
SmiMI CAYNNNNRTG 6 cut(s) 114, 879, 1448, 1650, 1737, 1764
SmlI CTYRAG 1 cut(s) 1967
SmoI CTYRAG 1 cut(s) 1967
SpeI ACTAGT 2 cut(s) 815, 1606
SseBI AGGCCT 1 cut(s) 1506
SsiI CCGC 2 cut(s) 1549, 1555
SspI AATATT 1 cut(s) 1086
SspMI CTAG 8 cut(s) 212, 795, 816, 855, 1511, 1607, 1839, 2109
SstI GAGCTC 1 cut(s) 1846
StuI AGGCCT 1 cut(s) 1506
StyD4I CCNGG 3 cut(s) 130, 867, 898
StyI CCWWGG 1 cut(s) 1501
SwaI ATTTAAAT 1 cut(s) 720
TaaI ACNGT 3 cut(s) 101, 390, 1583
TaiI ACGT 4 cut(s) 609, 778, 1637, 1665
TaqI TCGA 5 cut(s) 155, 1518, 1541, 1832, 2327
TatI WGTACW 2 cut(s) 1700, 1905
TfiI GAWTC 6 cut(s) 152, 371, 1130, 1655, 1752, 2081
Tru1I TTAA 7 cut(s) 719, 915, 1020, 1199, 1571, 1875, 2238
Tru9I TTAA 7 cut(s) 719, 915, 1020, 1199, 1571, 1875, 2238
TscAI CASTG 3 cut(s) 286, 393, 409
TseFI GTSAC 4 cut(s) 811, 881, 1182, 1266
TseI GCWGC 2 cut(s) 1742, 2282
Tsp45I GTSAC 4 cut(s) 811, 881, 1182, 1266
TspRI CASTG 3 cut(s) 286, 393, 409
VpaK11BI GGWCC 1 cut(s) 1034
XapI RAATTY 8 cut(s) 139, 440, 706, 735, 1353, 1514, 1695, 2382
XbaI TCTAGA 1 cut(s) 1838
XceI RCATGY 1 cut(s) 1862
XcmI CCANNNNNNNNNTGG 1 cut(s) 2399
XmnI GAANNNNTTC 3 cut(s) 9, 398, 444
XspI CTAG 8 cut(s) 212, 795, 816, 855, 1511, 1607, 1839, 2109
Zsp2I ATGCAT 2 cut(s) 1066, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.