RLG00000017540

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000413
Physical Location & Seq
Reverse (-)
17997509 .. 18008450
10942 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017540

Sequence Viewer

Length: 6528 bp
ATGGAACATGTTCATATACCATTGCTCTCTCTGCTTGTTTTTCTCCTCAACCTCTCATCCCTTCACTTTCTCTCTTTAGCCGATGATTATTACATCAACTGTGGCTCACATGATAATGCGAGCCTCACTACTGGCCAGAATTTCTCCGGGGAATCGAAGGCTGGTTACTCTTTCTCCAACAGCAAGGCTGTCAAAGACATCAACCAGTTACTAGATATAGCACCTCTATATAAGACAGCAAGAGGTTTCAGAAAAGCATTCTACTACCAGTTCAACATCGCTCAAAATGGTACTTACCTGGTACGCCTACATTTCTTAGCTTTCTACTCCTCCTCCTCAAATAATCTCTCCACAGCTGTTTTTGGTGTCTTGGATTCTAAGAATTTCACACTGTTGAACAATTTCACTGCCAAGAACAGTACCAACTACCCTGTGATAAAGGAATTCTTTGTCCGAATTGATATTACCGACTCCTTTAGACTATATTTTATTCCTCAACCATCATCTTTTGCATTTGTAAATGCCATTGAATTATTCCTTGCCCATTCAGATTTCATCCCTGAGAACAATATCAGTAATCTCCCTTTACATACAATTTACCGACTGAACGTTGGAGGCTCACCAGTAACTGACTCAATATGGAGAAACTGGGAAACAGATGATAGTCATCTTTCTGATCCAAACTCTGCAAAGAATGTCAGTTCTGGTACGCTCAATGAAAATTACTTGCCAGGACCAGTTGATGGTCTATTCCTATCCAACTATACTTTGGCCCCAATTCAGGTTTACCAGACTGCTAAACATATGAGTAACAGTAGTAGCAGGCCAGCCATATCCTCCAACGTAACATGGTTTTTTAGTGTGAGTAGGAAAGCTAGACACATTGTCCGGGCTCACTTCTGTGACATTATTGGTCAGCCTGGTGAGATTATATTTAACTTGTATTCAAATGGCAACTTCCGCAAGGAGATCGTCAGCAACATTTCTCTACATCCGGCTGTTCCTTTCTACTATGACTTTGTGGTGAATTCTAGTGAGTCTGAACTCATTAACATCACCATAAAACCTAAGAGTGAATCTGCAGATGCTTTTCTAAATGGTCTGGAAATATTGGAGATAGTGGAGGGATTAGCTCCAATTCCTACTGTGACAGACTCCAAGAAGAATATTGTGGCTCCTGTGGTTGGTTCAGTTCTTGGAGGCCTGTCACTCATATGCGTTTTAATAGTTGGATTTCTGTTCGGTTTCAGACACAGAAAGGCGAAAAAACGTGTGGAAACTTCAGTTTGGTCACCAATGCCTGCAAATGGAGGAGGGAGTTCTCACGGCTCCGCTCTCAATCTTAATTACCTTGGGTTGAAGATATCTTTTAATGAAATTCAGTCTGCAACAAACAACTTTGACACAAAGTTGGTGATAGGTAAGGGTGGCTTTGGGAATGTTTATAGAGGGACTCTCTTCAATGGTACAAAAGTGGCTGTGAAGCGAGCTTATAAGCGAGATGAGCATGGATCAGGTTCAGGCCAAGGTCTCCCAGAATTCGAAACAGAAATCATAGTGTTGTCGAAAATCCGCCACCGCCATCTTGTCTCCTTAATTGGTTACTGTAATGAAAGGTCTGAGATGATACTAGTGTATGAGTTCATGGAAAAAGGGACGTTGAGAGATCATTTGTATGATTCGGATGTGCCTCGCTTGTCCTGGAAGCAGAGACTTGAAATTTGTACTGGAGCAGCAAGGGGTCTTCATTATCTCCACACAGGTGCAGCTGGGGGAATCATTCACCGTGATGTCAAGTCCACCAACATATTGCTTGATAAAAACCATGTTGCCAAAGTTGCTGACTTTGGCCTTTCGAGATCTGGAGCTCTTGATGAAACGCATGTCAGCACTAATGTTAAAGGCACTTTTGGTTATCTTGATCCTGAGTACATGATGTCTGAACAGTTGACAGAAAAATCTGATGTTTACTCATTTGGTGTAGTTCTACTTGAAGTGTTGTGTGCAAGACCAGCTATTGATCCAACACTTCCAAGAGAGCAAATGAACTTAGCTGAATGGGGAATTCTTTGCCAGAAAAGAGGGGTTCTTGAAGAGATTGTTGATTCTCCACTAAAGGGTCAGATTGATCCTAACTCACTGCGTAAGTTTGGTGAGACGGTTGAGAAGTGTTTGCAAGAAGATGCTTGTGATAGGCCAACAATGGAGGATGTGCTGTGGGATTTGGAGTATGCATTACAGCTTCAGCAAACAACAAAGCTTAAAGAGGCTCATGAGGACAGCACAAACATTGATGCTTCATCAGCAGCATTTGCTTTGCCAAATGTTCAGCGTTTTCCTTCACTTGGTTCGACGAGGAATGGAGATGATGTCAGGGAAGCCGACTTGGACACAACAGAAAGTGAAATTTTCTCTCAATTGAAAATTGCTTATGAACTCCAAGAAAAGTATTTCGTCAATTGTGGATCAGATGCTAATCTGGACCTCAATGGTCGGAATTTCATTGGGGATTTGAAGCCTGATTCCTTCTCTTTCTCAAAGGGAGGTGAGATTGTAAAAGGCACCAACCTTACGATATCAAGTCTCTACCAGACAGCAAGAGTTTTCAGTCAACAATCCTACTACAAGTTCGAAATCACTGAAACCGGTACTTATCTGGTACGCCTGCACTTCTTTCCCTTTTCTTCGGCCTCAAGTAATCTTTCTGCTGGAATTTTTAATGTTTCGGATTCCAAGTTCACTCTGTTGAACAATTTCACTGCCAAGACTGCTAGTAACTCTGCTATAATAAAGGAATTCTTTCTTGGAATTAATAGTACCGACTCATTTAAACTATATTTTACACCTCAAGCATCATCTTTTGCTTTTGTAAACGCCATTGAAGTCTTCCTTGCACCTTCAAATTTCACCCCTGAGAATTACAACAGTAATCTTCCTTTACACACAACTTACCGAGTGAATGTTGGAGGCCCAGAGCTCTCACCAGATAATGACGTCACAATATGGAGAAACTGGGAAACAGATGATATTTATCTGTCTGATCCCAACTCTGCCAAGAATGTCTCTAACAAAAATACCCAGCTTAATTATACGTACGATAATACGGATCAAGCTGATGATTTTGTTGCATCTACTAAGTTTATTGCCCCGGATTTGGTTTACCAGACAGCTAAAGAGATGACTAGCAATTCCTCCAACATAACATGGTCTTTTAGTGTGAGCAGGAATGCTAGGCACATTGTCCGTGTACACTTCTGTGACATTGTTTCTGGTTCCCCTTATACTGTAGTGTTTGACTTGTACTCGAATGGCAACTTCCGTAAGCAAATCGTTGGACAGGAGTCCCCTTTTGCTCTTGTGGTGGCTACTCCTTTCTACTATGATTTTGTGGTGAATGCTAGTGAGTCTAAACTCATTAACATCAGGATAGCCCCTAACTTGAAGAATACAACAGAAAAAAATGCCTTTCTGAATGGGCTGGAAATCTTGGAGATATTGGAGGGAACAGCTCTAATTTATAATTTGGAAGAGTCCAAGAAGGTAAATGTGGCTCTTGTGGTTGGTTCAGTTCTTGGAGGAGGCCTGTTTCTTTTCATCATTGGATTCATGTTGTGTTTGAGACACAGAAAAGCGATAAAGAGAAAGAGAGGTGGGATTTCCCACAACCTTGGGTTGAAGATATCTTTTGCTCAAATCCGGTCTGCAACACACAACTTTGATCCGAAAATGCAGATAGGTGTGGGTGGTTTTGGGTATGTTTATAAAGGAACTCTCTCTAATGGCACCAAAGTGGCTGTGAAGCGTTGTAAGCAAGAGGGGCAAAGGTCAGGCCAAGGCCTTCCAGAATTCGAAACAGAAATCATAGTGTTGTCCAAAATCCGCCACCGCCATCTTGTCTCCTTAATTGGTTACTGTGATGAAGGGTCTGAGATGATACTAGTGTATGAGTTCATGGAAAAAGGGACCTTGAGAGATCATTTGTATGAATCGGATGTGCCTCGCTTGTCGTGGAAGCAAAGACTTGAAATTTGCATAGGTTCAGCAACTGGACTTGATTATCTCCATAAAGGTGCAGCAGGGGGCATCATCCACCGTGATGTGAAGTCCACCAACATATTGCTTGATGAGAACCTTGTTGCTAAAGTCGCTGACTTCGGGCTTTCGAGGTCTGGCCCTCTTGAAGAAACTCATGTCAGCACGGCTGTGAAAGGCACTATCGGTTACCTTGATCCCGAGTACATGATGTCACAGCAGTTGACAGAGAAGTCTGATGTGTACTCATTTGGTGTAGTTCTTCTTGAGGTGTTATGTGGAAGACCTGCTATTGATATAACGCTTCCAAGAGAGAAAGTGAACTTAGCTGAATGGGTTGTGCTGTGCAAGAAAGAAGGGTTGCTAGAAGAGGTCATTGACGTTTCATTGAAGGGTCATATTGATCCCAGCTCACTGAGACATTTTATTGAGACTGCAGAGAAGTGTTTGCAACACGATTCTTCTGATAGGCCCACTATGGCTGAGGTGCTGTGGGATTTGGACTATACATTGAAGCTTCATCAAACTGCGAGGCTTGGAGAAGCCCATGAGGACAGCACTACCGGTGCTTCATCAGCGTTTTTACTACCAAATATTCGGCATTTTGCTTCACTTGATTCGACAGTTAACAGAGCTGATATGAGGGACGATGAGATGGAGTCCACAGAAAGCGAAATTTTCTCCCAACTGAGAATCAGTGAGGCCAGACACCCTGTTGCAGCCTGTCCAATGGACTTGGTGAAAGCTCAGAGAAGCCGAAAATGTGAGAAGAGAAATGTTAATGTGACCCTCACTTCTGGCCAGAATTTCTCTGGGGAATCGAAGTCTGGTTACTCTTTCTCCAAAAGCAAGGCTGTCAAAGACATCAACCAGTTGCCAGATATATCACCTCTGTATAAGACAGCAAGATGTTTCAGTGAACCATTCTACTACCAGTTCAACATCGCTCAAAATGGTACTTATCTGGTACGCCTACATTTCTTGTCCTTTTCTTCCACCTCAAGTGATCTCTCCACAGCTGTTTTTGATGTTTTGGATTCTACGAATTTCACACTGTTGAACAATTTCACTGCCAAGAATAGTACTAGTTCTCCTTTGATAAAGGAGTTCTTTCTCAGAATCAATACTACTGACTCATTTAGACTGTATTTTACACCCCGGACATCATCTTTGGCATTTGTAAATGCCATTGAATTCTTAGCCATCCCTTCAGATTTCACCAACTTCATTCCTGAGAATTACACCAGCAATTTCCCTTTACAGACAATTTACAGGGTGAATGTTGGGGGCCCTTCACATAATGATACATTGTGGAGAAGCTGGGAAACGGATGACACTTACCTGCCTGATCCAAACTCTGCTAAGGATAGCAACAAAGGTACCCTTGGTAGTAGAAATTACTACGGAGAAATTGATGGTTTGGTTGCATCTAATGCGTCTGTTGCTCCGGAATGGGTTTACCAGACTGCTAAAGTGATGAATATCACTGCTAGCAGGCCAGCCAATTCCTCCAACATAACTAGGTCTTTTAGAGTGAGTGGGAATGCTAGACACATTGTCCGGGCTCACTTCTGTGACATTGTTGATCAGCCTTATAATGTTGTATTTAACTTGTATTCAAATGGCAACTTGCGCAAGGAGATTGGCAGCAGCAATAACACGATTTCTGGACGTTCCGCCTTTCCTTTCTACTATGATTTTATGGTGAGCCCTAGTGAGTCTGAACTCATTAACATCAGCATAAGACCTAACTTGGAAGATGCTACAGTTCTGAATGCATTTCTAAATGGGCTAGAAATATTGGAGATAATGGAGGGTGTAGCTTCAATTCCTAATGTGAAAGAGTCCAAGAAGAATATGGTGGCTCCTGTTGTTGGTCCAGTTCTTGGAGGCCTGTCACTCATCAGCGTTTTAATAGTTGGATTTCTGTTCGGTTTCAGACACAGAAAGGCGAAAAAACGTGAGGAAACTTCAGTTTGGTCACCAATGCCTGCAAATGGAGGAGGGAGTTCTCACGGCTCCGCTCTCAATCTTAATTATCTTGGGTTGAAGATATCTTTTAATGAAATTCAGTCTGCAACAAACAACTTTGACACAAAGTTGGTGATAGGCAAGGGTGGCTTTGGGAATGTTTATAGAGGGACTCTCTTCAATGGTACAAAAGTGGCTGTGAAGCGAGCTTATAAGCGAGATGAGCATGGATCAGGTTCAGGCCAAGGTCTCCCAGAATTCGAAACAGAAATCATAGTGTTGTCGAAAATCCGCCACCGCCATCTTGTCTCCTTAGTTGGTTACTGTAATGAAAGGTCTGAGATGATACTAGTGTATGAGTTCATGGAAAAAGGGACGTTGAGAGATCATTTGTATGATTCGGATGTGCCTCGCTTGTCATGGAAGCAAAGACTTGAAATTTGTACTGGTGCAGCAAGGGGTCTTCATTATCTGCACACAGGTGCAGCTGGGGGAATTGATAAGAGCATATTTATGCGATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

2176

Amino Acids

242.06

Weight (kDa)

6.4

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 31 - 114 3.7e-06 Malectin domain
Pkinase PF00069 470 - 739 1.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 471 - 740 1.7e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 1242 - 1508 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1243 - 1509 7.3e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 2044 - 2161 4e-14 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 2045 - 2161 5.9e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23200
fragaria_vesca FvH4_1g16860 FvH4_1g16861
malus_domestica MD02G1018900.v1.1 MD15G1289500.v1.1 MD15G1289600.v1.1 MD15G1289700.v1.1
prunus_persica Prupe.6G227800_v2.0.a1 Prupe.6G228000_v2.0.a1 Prupe.6G228100_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228200_v2.0.a1 Prupe.6G228300_v2.0.a1
pyrus_communis pycom02g01680 pycom02g01690 pycom15g25310 pycom15g25330 pycom15g25340
rosa_chinensis RchiOBHm_Chr1g0324321 RchiOBHm_Chr1g0324331 RchiOBHm_Chr1g0324341 RchiOBHm_Chr1g0324401 RchiOBHm_Chr1g0324441 RchiOBHm_Chr2g0106751 RchiOBHm_Chr2g0106761 RchiOBHm_Chr2g0106771 RchiOBHm_Chr2g0106781 RchiOBHm_Chr2g0106801 RchiOBHm_Chr2g0106811 RchiOBHm_Chr2g0106821
rosa_laevigata RLG00000017539 RLG00000017540 RLG00000017541 RLG00000030200 RLG00000030201
rosa_multiflora Rmu_sc0000751.1_g000001 Rmu_sc0000751.1_g000002 Rmu_sc0000751.1_g000003 Rmu_sc0000751.1_g000005 Rmu_sc0000751.1_g000007 Rmu_sc0000751.1_g000008 Rmu_sc0000751.1_g000018 Rmu_sc0000751.1_g000019 Rmu_sc0000751.1_g000026 Rmu_sc0000751.1_g000027 Rmu_sc0000751.1_g000028 Rmu_sc0000751.1_g000029 Rmu_sc0003410.1_g000012 Rmu_sc0004000.1_g000023 Rmu_sc0004000.1_g000024 Rmu_sc0004082.1_g000015 Rmu_sc0004082.1_g000017 Rmu_sc0026507.1_g000001 Rmu_sc0031196.1_g000001 Rmu_sc0039591.1_g000001
rosa_roxburghii Rroxscaffold_159G00432710 Rroxscaffold_159G00432780 Rroxscaffold_159G00432840 Rroxscaffold_159G00433010 Rroxscaffold_2G00098070 Rroxscaffold_2G00136770 Rroxscaffold_4G00325540 Rroxscaffold_4G00325630 Rroxscaffold_4G00325680
rosa_rugosa Rorug01G0045700 Rorug02G0138500 Rorug02G0138600 Rorug02G0138700 Rorug02G0138800 Rorug02G0138900
rosa_samantha Rh1AG061400 Rh1BG052300 Rh1CG063400 Rh1CG063700 Rh1DG067800 Rh2AG189400 Rh2AG189800 Rh2AG190000 Rh2AG190100 Rh2BG201100 Rh2BG201300 Rh2CG194500 Rh2CG194600 Rh2CG194700 Rh2DG195700 Rh2DG195900 Rh2DG196300 Rh2DG196400 Rh2DG196500 Rh2DG196600
rosa_wichuraiana Rw1G004230 Rw1G005210 Rw2G014900 Rw2G014910 Rw2G014930 Rw2G014940 Rw2G014950 Rw2G014960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 1494, 3519, 3762, 5628, 6216
AasI GACNNNNNNGTC 1 cut(s) 4264
AatII GACGTC 1 cut(s) 2997
Acc16I TGCGCA 1 cut(s) 5666
Acc36I ACCTGC 2 cut(s) 4327, 5382
Acc65I GGTACC 1 cut(s) 5411
AccB1I GGYRCC 3 cut(s) 2561, 3782, 5411
AccB7I CCANNNNNTGG 2 cut(s) 743, 5918
AccBSI CCGCTC 2 cut(s) 1334, 6056
AccIII TCCGGA 1 cut(s) 5479
AclI AACGTT 1 cut(s) 609
AcoI YGGCCR 2 cut(s) 133, 4831
AcuI CTGAAG 4 cut(s) 1266, 2228, 5226, 5988
AcyI GRCGYC 1 cut(s) 2994
AflIII ACRYGT 2 cut(s) 7, 1270
AgeI ACCGGT 2 cut(s) 2645, 4595
AhdI GACNNNNNGTC 2 cut(s) 884, 5588
AhlI ACTAGT 4 cut(s) 1630, 3937, 5117, 6352
AjnI CCWGG 4 cut(s) 297, 730, 919, 1700
AjuI GAANNNNNNNTTGG 2 cut(s) 2787, 2819
AleI CACNNNNGTG 7 cut(s) 900, 1761, 3255, 3788, 4202, 5604, 6483
AloI GAACNNNNNNTCC 2 cut(s) 2720, 2752
Alw21I GWGCWC 2 cut(s) 1870, 2979
AlwNI CAGNNNCTG 2 cut(s) 629, 4046
Ama87I CYCGRG 1 cut(s) 4232
Aor13HI TCCGGA 1 cut(s) 5479
ApaI GGGCCC 1 cut(s) 5324
ApeKI GCWGC 9 cut(s) 1733, 1766, 2306, 4073, 4751, 5679, 5682, 6455, 6488
ArsI GACNNNNNNTTYG 8 cut(s) 1704, 1736, 4011, 4043, 5185, 5217, 6426, 6458
AseI ATTAAT 1 cut(s) 2811
AsiGI ACCGGT 2 cut(s) 2645, 4595
Asp700I GAANNNNTTC 5 cut(s) 9, 401, 2753, 2799, 5096
Asp718I GGTACC 1 cut(s) 5411
AspLEI GCGC 1 cut(s) 5667
AsuC2I CCSGG 5 cut(s) 148, 890, 3149, 5191, 5594
AsuII TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
AsuNHI GCTAGC 1 cut(s) 5522
AvaI CYCGRG 1 cut(s) 4232
AvaII GGWCC 4 cut(s) 734, 2482, 3963, 5909
BaeGI GKGCMC 1 cut(s) 5324
BalI TGGCCA 2 cut(s) 135, 4833
BanI GGYRCC 3 cut(s) 2561, 3782, 5411
BanII GRGCYC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
BarI GAAGNNNNNNTAC 4 cut(s) 149, 181, 4847, 4879
BbsI GAAGAC 4 cut(s) 1736, 2878, 4321, 6458
Bbv12I GWGCWC 2 cut(s) 1870, 2979
BbvCI CCTCAGC 1 cut(s) 4515
BbvI GCAGC 9 cut(s) 1745, 1778, 2318, 4085, 4763, 5691, 5694, 6467, 6500
BccI CCATC 8 cut(s) 508, 737, 1590, 3897, 4681, 5243, 5441, 6312
BceAI ACGGC 3 cut(s) 1342, 4215, 6064
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BciT130I CCWGG 4 cut(s) 299, 732, 921, 1702
BclI TGATCA 1 cut(s) 5617
BcnI CCSGG 5 cut(s) 148, 890, 3149, 5191, 5594
BcuI ACTAGT 4 cut(s) 1630, 3937, 5117, 6352
BfmI CTRYAG 4 cut(s) 1080, 3285, 4467, 5796
BfuAI ACCTGC 2 cut(s) 4327, 5382
BglII AGATCT 1 cut(s) 1859
BisI GCNGC 9 cut(s) 1734, 1767, 2307, 4074, 4752, 5680, 5683, 6456, 6489
BlsI GCNGC 9 cut(s) 1735, 1768, 2308, 4075, 4753, 5681, 5684, 6457, 6490
BmcAI AGTACT 1 cut(s) 5116
Bme1390I CCNGG 9 cut(s) 148, 299, 732, 890, 921, 1702, 3149, 5191, 5594
Bme18I GGWCC 4 cut(s) 734, 2482, 3963, 5909
BmeRI GACNNNNNGTC 2 cut(s) 884, 5588
BmeT110I CYCGRG 1 cut(s) 4232
BmrFI CCNGG 9 cut(s) 148, 299, 732, 890, 921, 1702, 3149, 5191, 5594
BmrI ACTGGG 2 cut(s) 658, 3022
BmsI GCATC 9 cut(s) 1075, 2173, 2284, 2461, 2861, 3137, 4092, 5468, 5782
BmtI GCTAGC 1 cut(s) 5526
BmuI ACTGGG 2 cut(s) 658, 3022
BoxI GACNNNNGTC 1 cut(s) 3340
BpiI GAAGAC 4 cut(s) 1736, 2878, 4321, 6458
BplI GAGNNNNNCTC 4 cut(s) 1440, 1472, 6162, 6194
BpmI CTGGAG 2 cut(s) 1749, 1884
Bpu10I CCTNAGC 2 cut(s) 4515, 5394
Bpu14I TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
BpuEI CTTGAG 5 cut(s) 2677, 2832, 3988, 4319, 5017
BpuMI CCSGG 5 cut(s) 148, 890, 3149, 5191, 5594
BsaAI YACGTR 1 cut(s) 3093
BsaBI GATNNNNATC 4 cut(s) 666, 2475, 3030, 5513
BsaHI GRCGYC 1 cut(s) 2994
BsaI GGTCTC 2 cut(s) 1535, 6257
BsaJI CCNNGG 9 cut(s) 147, 1351, 1525, 3147, 3667, 3832, 5189, 5416, 6247
BsaWI WCCGGW 4 cut(s) 2645, 3696, 4595, 5479
Bse118I RCCGGY 2 cut(s) 2645, 4595
Bse3DI GCAATG 1 cut(s) 20
Bse8I GATNNNNATC 4 cut(s) 666, 2475, 3030, 5513
BseAI TCCGGA 1 cut(s) 5479
BseBI CCWGG 4 cut(s) 299, 732, 921, 1702
BseDI CCNNGG 9 cut(s) 147, 1351, 1525, 3147, 3667, 3832, 5189, 5416, 6247
BseJI GATNNNNATC 4 cut(s) 666, 2475, 3030, 5513
BseMI GCAATG 1 cut(s) 20
BseRI GAGGAG 7 cut(s) 35, 319, 322, 325, 1326, 3591, 6048
BseSI GKGCMC 1 cut(s) 5324
BseXI GCAGC 9 cut(s) 1745, 1778, 2318, 4085, 4763, 5691, 5694, 6467, 6500
BseYI CCCAGC 5 cut(s) 1769, 3078, 4439, 5352, 6491
BsgI GTGCAG 6 cut(s) 1785, 2651, 4092, 6461, 6474, 6507
BshNI GGYRCC 3 cut(s) 2561, 3782, 5411
BshTI ACCGGT 2 cut(s) 2645, 4595
BsiHKAI GWGCWC 2 cut(s) 1870, 2979
BsiHKCI CYCGRG 1 cut(s) 4232
BsiWI CGTACG 1 cut(s) 3093
BslFI GGGAC 7 cut(s) 1465, 1669, 3328, 3976, 4691, 6187, 6391
BsmBI CGTCTC 1 cut(s) 2150
BsmFI GGGAC 7 cut(s) 1465, 1669, 3328, 3976, 4691, 6187, 6391
BsmI GAATGC 5 cut(s) 257, 3232, 3400, 5581, 5812
Bso31I GGTCTC 2 cut(s) 1535, 6257
BsoBI CYCGRG 1 cut(s) 4232
Bsp119I TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
Bsp120I GGGCCC 1 cut(s) 5320
Bsp1286I GDGCHC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
Bsp13I TCCGGA 1 cut(s) 5479
Bsp1407I TGTACA 1 cut(s) 3247
BspEI TCCGGA 1 cut(s) 5479
BspHI TCATGA 1 cut(s) 2272
BspMAI CTGCAG 2 cut(s) 1084, 4471
BspMI ACCTGC 2 cut(s) 4327, 5382
BspOI GCTAGC 1 cut(s) 5526
BspT104I TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
BspT107I GGYRCC 3 cut(s) 2561, 3782, 5411
BspTNI GGTCTC 2 cut(s) 1535, 6257
BsrBI CCGCTC 2 cut(s) 1334, 6056
BsrDI GCAATG 1 cut(s) 20
BsrFI RCCGGY 2 cut(s) 2645, 4595
BsrGI TGTACA 1 cut(s) 3247
BssAI RCCGGY 2 cut(s) 2645, 4595
BssECI CCNNGG 9 cut(s) 147, 1351, 1525, 3147, 3667, 3832, 5189, 5416, 6247
BssNI GRCGYC 1 cut(s) 2994
BssT1I CCWWGG 6 cut(s) 1351, 1525, 3667, 3832, 5416, 6247
Bst2UI CCWGG 4 cut(s) 299, 732, 921, 1702
Bst6I CTCTTC 6 cut(s) 1463, 2088, 3522, 4395, 4796, 6185
BstACI GRCGYC 1 cut(s) 2994
BstAPI GCANNNNNTGC 2 cut(s) 2312, 5465
BstAUI TGTACA 1 cut(s) 3247
BstBAI YACGTR 1 cut(s) 3093
BstBI TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
BstEII GGTNACC 3 cut(s) 1290, 4220, 6012
BstHHI GCGC 1 cut(s) 5667
BstNI CCWGG 4 cut(s) 299, 732, 921, 1702
BstNSI RCATGY 2 cut(s) 11, 1886
BstPAI GACNNNNGTC 1 cut(s) 3340
BstPI GGTNACC 3 cut(s) 1290, 4220, 6012
BstSCI CCNGG 9 cut(s) 146, 297, 730, 888, 919, 1700, 3147, 5189, 5592
BstSFI CTRYAG 4 cut(s) 1080, 3285, 4467, 5796
BstSLI GKGCMC 1 cut(s) 5324
BstSNI TACGTA 1 cut(s) 3093
BstV1I GCAGC 9 cut(s) 1745, 1778, 2318, 4085, 4763, 5691, 5694, 6467, 6500
BstV2I GAAGAC 4 cut(s) 1736, 2878, 4321, 6458
BstX2I RGATCY 1 cut(s) 1859
BstXI CCANNNNNNTGG 1 cut(s) 3668
BstYI RGATCY 1 cut(s) 1859
BtgZI GCGATG 2 cut(s) 262, 4960
BtsI GCAGTG 5 cut(s) 405, 2138, 2757, 5100, 5517
BveI ACCTGC 2 cut(s) 4327, 5382
CaiI CAGNNNCTG 2 cut(s) 629, 4046
CciI TCATGA 1 cut(s) 2272
CfoI GCGC 1 cut(s) 5667
Cfr10I RCCGGY 2 cut(s) 2645, 4595
CseI GACGC 1 cut(s) 5457
CsiI ACCWGGT 1 cut(s) 297
CspAI ACCGGT 2 cut(s) 2645, 4595
DraI TTTAAA 1 cut(s) 2830
DrdI GACNNNNNNGTC 1 cut(s) 4264
DriI GACNNNNNGTC 2 cut(s) 884, 5588
DseDI GACNNNNNNGTC 1 cut(s) 4264
EaeI YGGCCR 2 cut(s) 133, 4831
Eam1104I CTCTTC 6 cut(s) 1463, 2088, 3522, 4395, 4796, 6185
Eam1105I GACNNNNNGTC 2 cut(s) 884, 5588
EarI CTCTTC 6 cut(s) 1463, 2088, 3522, 4395, 4796, 6185
EciI GGCGGA 4 cut(s) 1562, 3869, 5698, 6284
Ecl136II GAGCTC 2 cut(s) 1868, 2977
Eco105I TACGTA 1 cut(s) 3093
Eco130I CCWWGG 6 cut(s) 1351, 1525, 3667, 3832, 5416, 6247
Eco147I AGGCCT 4 cut(s) 1203, 3582, 3837, 5925
Eco24I GRGCYC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
Eco31I GGTCTC 2 cut(s) 1535, 6257
Eco32I GATATC 4 cut(s) 1365, 2577, 3681, 6087
Eco47I GGWCC 4 cut(s) 734, 2482, 3963, 5909
Eco53kI GAGCTC 2 cut(s) 1868, 2977
Eco57I CTGAAG 4 cut(s) 1266, 2228, 5226, 5988
Eco88I CYCGRG 1 cut(s) 4232
Eco91I GGTNACC 3 cut(s) 1290, 4220, 6012
EcoICRI GAGCTC 2 cut(s) 1868, 2977
EcoO109I RGGNCCY 3 cut(s) 3963, 5320, 5321
EcoO65I GGTNACC 3 cut(s) 1290, 4220, 6012
EcoRI GAATTC 8 cut(s) 443, 1027, 1538, 2064, 2795, 3845, 5225, 6260
EcoRII CCWGG 4 cut(s) 297, 730, 919, 1700
EcoRV GATATC 4 cut(s) 1365, 2577, 3681, 6087
EcoT14I CCWWGG 6 cut(s) 1351, 1525, 3667, 3832, 5416, 6247
EcoT22I ATGCAT 2 cut(s) 2236, 5812
EcoT38I GRGCYC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
ErhI CCWWGG 6 cut(s) 1351, 1525, 3667, 3832, 5416, 6247
Esp3I CGTCTC 1 cut(s) 2150
FaqI GGGAC 7 cut(s) 1465, 1669, 3328, 3976, 4691, 6187, 6391
FauNDI CATATG 2 cut(s) 804, 1214
FbaI TGATCA 1 cut(s) 5617
Fnu4HI GCNGC 9 cut(s) 1734, 1767, 2307, 4074, 4752, 5680, 5683, 6456, 6489
FriOI GRGCYC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
Fsp4HI GCNGC 9 cut(s) 1734, 1767, 2307, 4074, 4752, 5680, 5683, 6456, 6489
FspI TGCGCA 1 cut(s) 5666
GlaI GCGC 1 cut(s) 5666
GluI GCNGC 9 cut(s) 1734, 1767, 2307, 4074, 4752, 5680, 5683, 6456, 6489
GsaI CCCAGC 5 cut(s) 1773, 3082, 4443, 5356, 6495
GsuI CTGGAG 2 cut(s) 1749, 1884
HgaI GACGC 1 cut(s) 5457
HhaI GCGC 1 cut(s) 5667
Hin1I GRCGYC 1 cut(s) 2994
Hin6I GCGC 1 cut(s) 5665
HinP1I GCGC 1 cut(s) 5665
HincII GTYRAC 4 cut(s) 1950, 2612, 4257, 4660
HindII GTYRAC 4 cut(s) 1950, 2612, 4257, 4660
HindIII AAGCTT 2 cut(s) 2258, 4547
HpaI GTTAAC 1 cut(s) 4660
Hpy99I CGWCG 1 cut(s) 2356
Hsp92I GRCGYC 1 cut(s) 2994
HspAI GCGC 1 cut(s) 5665
Kpn2I TCCGGA 1 cut(s) 5479
KpnI GGTACC 1 cut(s) 5415
Ksp22I TGATCA 1 cut(s) 5617
KspAI GTTAAC 1 cut(s) 4660
LmnI GCTCC 8 cut(s) 1138, 1180, 1334, 1730, 1865, 5482, 5902, 6056
Lsp1109I GCAGC 9 cut(s) 1745, 1778, 2318, 4085, 4763, 5691, 5694, 6467, 6500
LweI GCATC 9 cut(s) 1075, 2173, 2284, 2461, 2861, 3137, 4092, 5468, 5782
MabI ACCWGGT 1 cut(s) 297
MbiI CCGCTC 2 cut(s) 1334, 6056
MfeI CAATTG 2 cut(s) 2417, 2458
MflI RGATCY 1 cut(s) 1859
MhlI GDGCHC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
MlsI TGGCCA 2 cut(s) 135, 4833
MluNI TGGCCA 2 cut(s) 135, 4833
Mox20I TGGCCA 2 cut(s) 135, 4833
Mph1103I ATGCAT 2 cut(s) 2236, 5812
MroI TCCGGA 1 cut(s) 5479
MroXI GAANNNNTTC 5 cut(s) 9, 401, 2753, 2799, 5096
MscI TGGCCA 2 cut(s) 135, 4833
Msp20I TGGCCA 2 cut(s) 135, 4833
MspA1I CMGCKG 4 cut(s) 356, 1769, 5051, 6491
MspR9I CCNGG 9 cut(s) 148, 299, 732, 890, 921, 1702, 3149, 5191, 5594
MunI CAATTG 2 cut(s) 2417, 2458
Mva1269I GAATGC 5 cut(s) 257, 3232, 3400, 5581, 5812
MvaI CCWGG 4 cut(s) 299, 732, 921, 1702
NciI CCSGG 5 cut(s) 148, 890, 3149, 5191, 5594
NdeI CATATG 2 cut(s) 804, 1214
NheI GCTAGC 1 cut(s) 5522
NsbI TGCGCA 1 cut(s) 5666
NsiI ATGCAT 2 cut(s) 2236, 5812
NspI RCATGY 2 cut(s) 11, 1886
NspV TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
OliI CACNNNNGTG 7 cut(s) 900, 1761, 3255, 3788, 4202, 5604, 6483
PagI TCATGA 1 cut(s) 2272
PceI AGGCCT 4 cut(s) 1203, 3582, 3837, 5925
PciI ACATGT 1 cut(s) 7
PctI GAATGC 5 cut(s) 257, 3232, 3400, 5581, 5812
PdmI GAANNNNTTC 5 cut(s) 9, 401, 2753, 2799, 5096
Pfl23II CGTACG 1 cut(s) 3093
PflMI CCANNNNNTGG 2 cut(s) 743, 5918
PfoI TCCNGGA 1 cut(s) 1700
PinAI ACCGGT 2 cut(s) 2645, 4595
PkrI GCNGC 9 cut(s) 1735, 1768, 2308, 4075, 4753, 5681, 5684, 6457, 6490
Ppu21I YACGTR 1 cut(s) 3093
PpuMI RGGWCCY 1 cut(s) 3963
PscI ACATGT 1 cut(s) 7
PshAI GACNNNNGTC 1 cut(s) 3340
PshBI ATTAAT 1 cut(s) 2811
PsiI TTATAA 5 cut(s) 1494, 3519, 3762, 5628, 6216
Psp124BI GAGCTC 2 cut(s) 1870, 2979
Psp1406I AACGTT 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 3963
Psp6I CCWGG 4 cut(s) 297, 730, 919, 1700
PspEI GGTNACC 3 cut(s) 1290, 4220, 6012
PspFI CCCAGC 5 cut(s) 1769, 3078, 4439, 5352, 6491
PspGI CCWGG 4 cut(s) 297, 730, 919, 1700
PspLI CGTACG 1 cut(s) 3093
PspOMI GGGCCC 1 cut(s) 5320
PspPPI RGGWCCY 1 cut(s) 3963
PstI CTGCAG 2 cut(s) 1084, 4471
PstNI CAGNNNCTG 2 cut(s) 629, 4046
PsuI RGATCY 1 cut(s) 1859
PvuII CAGCTG 4 cut(s) 356, 1769, 5051, 6491
SacI GAGCTC 2 cut(s) 1870, 2979
SatI GCNGC 9 cut(s) 1734, 1767, 2307, 4074, 4752, 5680, 5683, 6456, 6489
ScaI AGTACT 1 cut(s) 5116
ScrFI CCNGG 9 cut(s) 148, 299, 732, 890, 921, 1702, 3149, 5191, 5594
SduI GDGCHC 6 cut(s) 895, 1870, 2979, 5324, 5599, 5744
SexAI ACCWGGT 1 cut(s) 297
SfaNI GCATC 9 cut(s) 1075, 2173, 2284, 2461, 2861, 3137, 4092, 5468, 5782
SfcI CTRYAG 4 cut(s) 1080, 3285, 4467, 5796
SfuI TTCGAA 4 cut(s) 1542, 2631, 3849, 6264
SinI GGWCC 4 cut(s) 734, 2482, 3963, 5909
SmlI CTYRAG 5 cut(s) 2692, 2847, 3967, 4298, 5032
SmoI CTYRAG 5 cut(s) 2692, 2847, 3967, 4298, 5032
SnaBI TACGTA 1 cut(s) 3093
SpeI ACTAGT 4 cut(s) 1630, 3937, 5117, 6352
SseBI AGGCCT 4 cut(s) 1203, 3582, 3837, 5925
SspI AATATT 4 cut(s) 1110, 1168, 4627, 5832
SstI GAGCTC 2 cut(s) 1870, 2979
StuI AGGCCT 4 cut(s) 1203, 3582, 3837, 5925
StyD4I CCNGG 9 cut(s) 146, 297, 730, 888, 919, 1700, 3147, 5189, 5592
StyI CCWWGG 6 cut(s) 1351, 1525, 3667, 3832, 5416, 6247
TatI WGTACW 8 cut(s) 1724, 1929, 3247, 3300, 4236, 4275, 5114, 6446
TseI GCWGC 9 cut(s) 1733, 1766, 2306, 4073, 4751, 5679, 5682, 6455, 6488
TspGWI ACGGA 5 cut(s) 3119, 3233, 3308, 5375, 5451
Van91I CCANNNNNTGG 2 cut(s) 743, 5918
VpaK11BI GGWCC 4 cut(s) 734, 2482, 3963, 5909
VspI ATTAAT 1 cut(s) 2811
XceI RCATGY 2 cut(s) 11, 1886
XcmI CCANNNNNNNNNTGG 3 cut(s) 766, 4841, 5887
XmnI GAANNNNTTC 5 cut(s) 9, 401, 2753, 2799, 5096
ZraI GACGTC 1 cut(s) 2995
ZrmI AGTACT 1 cut(s) 5116
Zsp2I ATGCAT 2 cut(s) 2236, 5812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.