MD12G1007800.v1.1

Stellacyanin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000318
Physical Location & Seq
Reverse (-)
843843 .. 844610
768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1007800.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCTTCCAACAAATTTGTCACCCTAGTAACCCTTGTTATGATCCTTCTTCCTACAATCGCCATGGCAACCGACTTCATCGTCGGAGGTGATAGCGGCTGGACAACTGGTGTTGATTATTCAAATTGGGCCAAGGACAAAACGTTTCATGTTGGTGATGCACTAGTGTTCAAGTACTCGAACCCACCCCACAATGTGTTCAAGGTGAACGGAACTGGCTTCAAGGAATGTGTTAAACCAACAGCAAATGATCAAGCACCACTCACTTCTGGAAACGACAGAATAGAGCTGAAGACTCCTGGAAACAAATGGTACATTTGTACAGCTGCCAATCACTGTGCTGATTTGGAACAGAAGCTTGTTATTACTGTTATGGACGCCGCGCCTGCGCCCGCTCCATCCTCCGCCGTTCGCGGGATTATCTTTTCCGGATACCAAGTCTTCGCGGCCGCCGTAGTCGGAGTCTTCCTTGCTGTTGCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

162

Amino Acids

17.14

Weight (kDa)

6.81

Isoelectric Point (pI)

21.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 114 8.8e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17675
fragaria_vesca FvH4_4g34040 FvH4_5g01010 FvH4_5g01020 FvH4_5g01030 FvH4_5g01040 FvH4_6g39940
malus_domestica MD04G1075600.v1.1 MD04G1075800.v1.1 MD04G1075900.v1.1 MD06G1068300.v1.1 MD12G1007700.v1.1 MD12G1007800.v1.1 MD12G1007900.v1.1 MD14G1005400.v1.1
prunus_persica Prupe.5G084000_v2.0.a1 Prupe.5G084100_v2.0.a1 Prupe.5G084200_v2.0.a1 Prupe.5G084400_v2.0.a1 Prupe.7G008700_v2.0.a1 Prupe.7G009100_v2.0.a1
pyrus_communis pycom04g06640 pycom04g06650 pycom06g06620
rosa_chinensis RchiOBHm_Chr2g0154451 RchiOBHm_Chr2g0154471 RchiOBHm_Chr4g0443531 RchiOBHm_Chr7g0200311 RchiOBHm_Chr7g0200321 RchiOBHm_Chr7g0200341 RchiOBHm_Chr7g0200371
rosa_laevigata RLG00000003732 RLG00000003733 RLG00000003734 RLG00000003735 RLG00000003736 RLG00000003737 RLG00000005919 RLG00000020779
rosa_multiflora Rmu_co8434485.1_g000001 Rmu_sc0000648.1_g000005 Rmu_sc0001118.1_g000024 Rmu_sc0003808.1_g000012 Rmu_sc0005350.1_g000004 Rmu_sc0005350.1_g000005 Rmu_sc0005350.1_g000009 Rmu_sc0005350.1_g000010 Rmu_sc0005350.1_g000011 Rmu_sc0006475.1_g000003
rosa_roxburghii Rroxscaffold_2G00094310 Rroxscaffold_2G00094320 Rroxscaffold_3G00256060 Rroxscaffold_3G00256070 Rroxscaffold_3G00256090 Rroxscaffold_3G00256100 Rroxscaffold_3G00256110 Rroxscaffold_3G00256150 Rroxscaffold_3G00256160 Rroxscaffold_5G00384080
rosa_rugosa Rorug02G0444500 Rorug02G0444600 Rorug04G0347000 Rorug07G0059100 Rorug07G0059200 Rorug07G0059300 Rorug07G0059400 Rorug07G0059500 Rorug07G0059600 Rorug07G0059700 Rorug07G0059800
rosa_samantha Rh2AG509000 Rh2AG509100 Rh2BG520300 Rh2BG520400 Rh2CG494400 Rh2CG494500 Rh2DG530600 Rh2DG530700 Rh4AG402700 Rh4BG414400 Rh4CG430000 Rh4DG409900 Rh4DG411200 Rh7AG184500 Rh7AG184600 Rh7AG184700 Rh7AG184800 Rh7AG184900 Rh7AG185000 Rh7BG187200 Rh7BG187300 Rh7BG187400 Rh7BG187800 Rh7BG187900 Rh7BG188000 Rh7BG188100 Rh7CG194900 Rh7CG195000 Rh7CG195100 Rh7CG195200 Rh7CG195300 Rh7DG188500 Rh7DG188600 Rh7DG188700 Rh7DG188800 Rh7DG188900 Rh7DG189100
rosa_wichuraiana Rw2G041800 Rw2G041810 Rw4G034720 Rw7G016160 Rw7G016170 Rw7G016180 Rw7G016190 Rw7G016200 Rw7G016210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 395
AccII CGCG 3 cut(s) 383, 414, 446
AccIII TCCGGA 1 cut(s) 428
AciI CCGC 7 cut(s) 96, 381, 393, 405, 414, 446, 450
AclI AACGTT 1 cut(s) 143
AclWI GGATC 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 3 cut(s) 176, 314, 322
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 4 cut(s) 123, 172, 202, 223
AhlI ACTAGT 1 cut(s) 163
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 3 cut(s) 289, 326, 358
AluI AGCT 3 cut(s) 289, 326, 358
AlwI GGATC 1 cut(s) 37
Aor13HI TCCGGA 1 cut(s) 428
AoxI GGCC 2 cut(s) 129, 447
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 1 cut(s) 14
AspLEI GCGC 2 cut(s) 385, 391
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 4 cut(s) 13, 101, 167, 217
BaeI ACNNNNGTAYC 2 cut(s) 304, 337
BbsI GAAGAC 3 cut(s) 299, 433, 457
BbvI GCAGC 1 cut(s) 313
BccI CCATC 1 cut(s) 406
BceAI ACGGC 2 cut(s) 392, 437
BciT130I CCWGG 1 cut(s) 300
BciVI GTATCC 1 cut(s) 425
BclI TGATCA 1 cut(s) 250
BcuI ACTAGT 1 cut(s) 163
BfaI CTAG 2 cut(s) 26, 164
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 5 cut(s) 97, 327, 381, 447, 450
BlsI GCNGC 5 cut(s) 98, 328, 382, 448, 451
BmcAI AGTACT 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 148
BpiI GAAGAC 3 cut(s) 299, 433, 457
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 63, 132
BsaWI WCCGGW 1 cut(s) 428
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 2 cut(s) 112, 220
BseAI TCCGGA 1 cut(s) 428
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 63, 132
BseGI GGATG 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 112, 220
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 1 cut(s) 313
Bsh1236I CGCG 3 cut(s) 383, 414, 446
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 2 cut(s) 131, 449
BsiEI CGRYCG 1 cut(s) 450
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 414
BsnI GGCC 2 cut(s) 131, 449
Bsp13I TCCGGA 1 cut(s) 428
Bsp1407I TGTACA 1 cut(s) 320
Bsp143I GATC 2 cut(s) 42, 250
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 7 cut(s) 96, 381, 393, 405, 414, 446, 450
BspANI GGCC 2 cut(s) 131, 449
BspEI TCCGGA 1 cut(s) 428
BspFNI CGCG 3 cut(s) 383, 414, 446
BspPI GGATC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 320
BsrI ACTGG 2 cut(s) 112, 220
BssECI CCNNGG 2 cut(s) 63, 132
BssMI GATC 2 cut(s) 42, 250
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 63, 132
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 338, 370
BstACI GRCGYC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 320
BstC8I GCNNGC 2 cut(s) 387, 393
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 398
BstFNI CGCG 3 cut(s) 383, 414, 446
BstHHI GCGC 2 cut(s) 385, 391
BstKTI GATC 2 cut(s) 45, 253
BstMBI GATC 2 cut(s) 42, 250
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstUI CGCG 3 cut(s) 383, 414, 446
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 3 cut(s) 299, 433, 457
BstZI CGGCCG 1 cut(s) 447
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 2 cut(s) 131, 449
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 398
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 2 cut(s) 387, 393
CciNI GCGGCCGC 1 cut(s) 447
CfoI GCGC 2 cut(s) 385, 391
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 1 cut(s) 386
Csp6I GTAC 3 cut(s) 175, 313, 321
CviAII CATG 2 cut(s) 64, 149
CviJI RGCY 8 cut(s) 5, 99, 131, 219, 289, 326, 358, 449
CviKI_1 RGCY 8 cut(s) 5, 99, 131, 219, 289, 326, 358, 449
CviQI GTAC 3 cut(s) 175, 313, 321
DpnI GATC 2 cut(s) 44, 252
DpnII GATC 2 cut(s) 42, 250
DraIII CACNNNGTG 1 cut(s) 196
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
EciI GGCGGA 1 cut(s) 394
EclXI CGGCCG 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 63, 132
Eco52I CGGCCG 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 63, 132
ErhI CCWWGG 2 cut(s) 63, 132
FaeI CATG 2 cut(s) 67, 152
FaiI YATR 4 cut(s) 41, 65, 150, 374
FatI CATG 2 cut(s) 63, 148
FauI CCCGC 2 cut(s) 400, 407
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 5 cut(s) 97, 327, 381, 447, 450
FokI GGATG 1 cut(s) 385
Fsp4HI GCNGC 5 cut(s) 97, 327, 381, 447, 450
FspBI CTAG 2 cut(s) 26, 164
GlaI GCGC 2 cut(s) 384, 390
GluI GCNGC 5 cut(s) 97, 327, 381, 447, 450
HaeIII GGCC 2 cut(s) 131, 449
HapII CCGG 1 cut(s) 429
HgaI GACGC 1 cut(s) 386
HhaI GCGC 2 cut(s) 385, 391
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 67, 152
Hin6I GCGC 2 cut(s) 383, 389
HinP1I GCGC 2 cut(s) 383, 389
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 2 cut(s) 295, 462
HpaII CCGG 1 cut(s) 429
HphI GGTGA 4 cut(s) 13, 101, 167, 217
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 3 cut(s) 86, 461, 485
Hpy188III TCNNGA 2 cut(s) 270, 429
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 338, 370
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 2 cut(s) 161, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 67, 152
HspAI GCGC 2 cut(s) 383, 389
Kpn2I TCCGGA 1 cut(s) 428
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 42, 250
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 8 cut(s) 85, 93, 201, 255, 285, 312, 399, 442
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 26, 164
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 19, 28
MalI GATC 2 cut(s) 44, 252
MbiI CCGCTC 1 cut(s) 395
MboI GATC 2 cut(s) 42, 250
MboII GAAGA 4 cut(s) 41, 304, 433, 457
MluCI AATT 2 cut(s) 14, 124
MlyI GAGTC 2 cut(s) 289, 471
MmeI TCCRAC 3 cut(s) 33, 64, 439
MnlI CCTC 2 cut(s) 80, 412
MroI TCCGGA 1 cut(s) 428
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 3 cut(s) 383, 414, 446
MwoI GCNNNNNNNGC 1 cut(s) 386
NcoI CCATGG 1 cut(s) 63
NdeII GATC 2 cut(s) 42, 250
NlaIII CATG 2 cut(s) 67, 152
NmuCI GTSAC 1 cut(s) 19
NotI GCGGCCGC 1 cut(s) 447
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PfoI TCCNGGA 1 cut(s) 298
PkrI GCNGC 5 cut(s) 98, 328, 382, 448, 451
PleI GAGTC 2 cut(s) 289, 470
PpsI GAGTC 2 cut(s) 289, 470
Psp1406I AACGTT 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspPI GGNCC 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 326
RsaI GTAC 3 cut(s) 176, 314, 322
RsaNI GTAC 3 cut(s) 175, 313, 321
RseI CAYNNNNRTG 1 cut(s) 153
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 5 cut(s) 97, 327, 381, 447, 450
Sau3AI GATC 2 cut(s) 42, 250
Sau96I GGNCC 1 cut(s) 129
ScaI AGTACT 1 cut(s) 176
SchI GAGTC 2 cut(s) 289, 471
ScrFI CCNGG 1 cut(s) 300
SetI ASST 6 cut(s) 91, 146, 207, 291, 328, 360
SfaNI GCATC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 153
SpeI ACTAGT 1 cut(s) 163
Sse9I AATT 2 cut(s) 14, 124
SsiI CCGC 7 cut(s) 96, 381, 393, 405, 414, 446, 450
SspMI CTAG 2 cut(s) 26, 164
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 2 cut(s) 63, 132
TaaI ACNGT 2 cut(s) 338, 370
TaiI ACGT 1 cut(s) 146
TaqI TCGA 1 cut(s) 179
TasI AATT 2 cut(s) 14, 124
TatI WGTACW 2 cut(s) 174, 320
TauI GCSGC 4 cut(s) 99, 383, 449, 452
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 1 cut(s) 341
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 67, 137
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 1 cut(s) 341
XapI RAATTY 1 cut(s) 14
XspI CTAG 2 cut(s) 26, 164
ZrmI AGTACT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.