Rorug07G0059200

Blue copper

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000318
Physical Location & Seq
Forward (+)
4258724 .. 4263650
4927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0059200.1

Sequence Viewer

Length: 2895 bp
ATGAAGGTCCGGCAAGTGAAACTCCGCGAGATCCACAAATCGGCGAGCAGCGCCGGAAAGCCGTCCTTCTGCTCCGTCGTGTGGGACCAGCAAGGCTTCCATTCCGTCACCGCCTCCTCCTCCGACGCCTCAATCGCCGTCCACAACGCTCTACTCTCCACATGTCCGCCGAAGCTCCTCCGCAACCACCGCGACGGCGTCACGGCGCTCGCTATGAGCCCCAACGCCACCTGCCTCGCCTCCGGCTCCGTCGACCACTCCGTCAAGCTCTACAAGTTTCCTAGTGGAGAGTTTGAGACTAATATTACCAGATTCACGCTGCCAATACGTGCCCTTGCGTTCAATAAGTCAGGGACACTCTTGGCCGCTGCTGGTGATGATGATGGCATTAAACTTATTAACACAGTAGACGGTACAATTTCAAAGGTTCTCAAAGCACACAAAGGACCTGTAACCGGCTTAGGTTTTGATCCCCAAAGTGAATACATGGCATCCATCGATTCAACTGGGACTGTAGTATACTGGGAACTCCAATCTGGAAGCACATTGCACACCCTTAAAAGCGTAGCTCCTAACTCTGGTTTAGACCTTTCTGTGTTGAATGTACTGAGCTGGAGTCCTGATGGACGGATGTTAGCTGTTCCAGGAGTGAGAAATGATGTTGTGGTGTATGATAAAGACACTGCTGAGAAGCTCTTTACATTGAGAGGTGAACATACACAGTCTATTTGTGCCTTGGCTTGGTCTCCTAATGGAAAATACTTGGCTACCTCTGGCTTGGATAAGCAGGTTCTCATCTGGGATGTTGATCGGAAGCAGGATATTGGCAGGCATAAATTTGATGAGAGGATAACTTGCTTGGCGTGGAAGCCAATTGACAATGTCCTAGCTGTAATTGATTGTATGGGCAGGTATGGTGTGTGGGAAGCAGTGATTCCTTCATCAATGAAATCTCCCACTGAAGATGTTCCGAATCAATCTAATAATGGTCTACTTTTGTTTGATGAAGACGAAGACGAGGGTCAGGATATTAGTACATCTGGTAGTTTAAGTGATCTTGGTGAAGATACATATGAGGAGTCTAAGCCACCTAGCCGGAAAAGACTAAGCAAGCAGTCTGACTCTCTGAGAGATTTAGATGAGGATGATAATGATGACTTTGGTCTGTCCCCTAAAGTTGAATCCCATAAAAAAGCACGTCGGAATCATAGTGAAAGCACTGACAATGGGAATGAGGGATTAAGAAGCAGACTGTCATCTGCCAGGCCAAAAATGCAGGAGGCATTTCAGCCAGGGGCCACTCCTGTGCAGCCTGGAAAAAGGCGCTTTTTGTGCTACAACATGCTTGGAGCTATAACTACGATTGAATACGATGGGTACTCCCATATTGAGATAGATTTTCATGATACAAGCCGAGGTCCACGAGTTCCATCAATGACAGACCATTTTGGTTTTACAATGGCTTCACTGAATGAGAATGGAAGTGTTTTTGCAAATCCCTGCAAGGGTGAAAAGAACATGAGCACTCTCATGTATCGCCCATTTAGTAGCTGGGCAAACAATAGCGAGTGGTCTATGCGATTGGAAGGGGAAGAAGTGAAGGTTGTGGCACTTGGTACAACTTGGGTGGCAGCAATTACTAGTCTTAACTTGATCCGCATCTTCACCATTGGTGGTTTGCAGAGACATGTTCTCTCCCTCGATGGACCTGTGGTGACTGCATCAGGCTTCATGGATGAGCTTGCCATTGTCACTCATGCTTCAGACAGTCTTCCCTCAAATGAACAGGTGCTTGAATTTAGGGTACTTAACATAAATAATAGTAGCCAGCCTCTTAGAGGACGTCTGCCTCTGACTCCTGGTTCAAGTTTAACATGGTTTGGCTTTAGTGAAGAAGGGAAATTAAGCTCCTATGACTCCAAGGGTGTGCTGAGAGTTTTTACAAGTGAATATGGTGGCAGTTGGTTCCCGCTCTTCAGTGCTAGTAAAGACAAGAAGTCAGATGAAAACTATTGGGTGATCGGTTTGAATGCAAGACAACTATTTTGTATAGTTTGCAAAAGCCCCGAGTTATATCCACAGGTGTTGCCCAAACCAGTGCTCACTCTACTAAATTTTTCAATTCCTCTTGCATCCTCTGATCTAGGAGCAGAAGCCCTTGAAAATGCATTTATACTAAACGACATGCATCTCGTACAGATTCAAAAGCAAATAGAAGAAATGGATGCTGCAGGTTTAGACACCAGTTCACTTGATGATGAAGCTTTTAAATTGGAAACAGCTCAAGATCAATGTATATTGAGGCTTATTGGATCCTGCTGCCATGGAGATAAGCTTGTGAGGGCTACTGAACTTGTGAAGCATCTATCCCTGGAAAAATCAGTTAGGGGTGCAATTAAGATTGTTACTTCTCTGAAGCTACCTAACTTGGCTGAAAGGTTCAATAGCATATTGGAGGAAAGGTTGCTCAATGATAGTAAAGAACCATTGCAAACAACTGTCCCAAACCCAAACTACAATGCATCATCCTCTACCGAGAAAAGCAAGTTATCAGGGAATGATATTCCATTACCAGCACCAAAATTGTCAGCTCCTACGTTTCTAAAGAAAGCCAAAACACAAGAAGCTAATGTTGGAACAGCGAAAACACAAGAAAAACAAACTGATCTTGAGAAGTCGACGACGACTGAGAGGGAAACTGCTGGTGCAGAGGTGAATAATGCAGCAGGTGACATGAAGAAGGTAGGGGAAATGCCTAAGGCACAACAGTCACATCGCCCGTCTAATCCATTTCTAAAGCGATCAAACAGTCAAGACATAGAGAAGAAGGAAGAAGTAGATCAGGTGAAAGCTCACCGCCCATCGAATCCGTTCTTCAAGAAATCGGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

964

Amino Acids

105.87

Weight (kDa)

6.03

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 9 - 138 1e-14 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 22 - 142 3.3e-10 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 25 - 307 4.3e-69 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 25 - 187 3e-23 WDR5 beta-propeller domain
WD40_Gbeta PF25391 36 - 157 5.8e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 37 - 186 1.3e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 59 - 91 5.5e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 59 - 187 1.1e-18 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 62 - 278 5.9e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 198 4.6e-10 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 64 - 298 1.6e-32 Prp19 WD40 domain
WDR55 PF24796 64 - 188 2.7e-10 WDR55
Beta-prop_WDR3_2nd PF25172 64 - 187 1e-10 WDR3 second beta-propeller domain
Beta-prop_Aladin PF25460 107 - 269 4.1e-10 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 116 - 270 7.9e-14 WDR19 first beta-propeller
Beta-prop_THOC3 PF25174 142 - 234 8.8e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 144 - 277 3.2e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 171 - 294 1.2e-11 WDR3 first beta-propeller domain
Beta-prop_IFT122_1st PF23381 200 - 310 2e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 201 - 299 9.1e-15 THOC3 beta-propeller domain
Beta-prop_IFT140_1st PF23383 201 - 285 1.9e-07 IFT140 first beta-propeller
Beta-prop_WDR5 PF25175 202 - 297 5.7e-12 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 203 - 296 3.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 203 - 277 1e-06 CAF1B/HIR1 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 2e-07 Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 205 - 299 5.3e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 230 - 302 8.6e-06 Aladin seven-bladed propeller
Beta-prop_TEP1_2nd PF25047 231 - 290 6.6e-06 TEP-1 second beta-propeller
WD40 PF00400 232 - 268 2.7e-08 WD domain, G-beta repeat
Mcl1_mid PF12341 427 - 710 4.9e-95 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 720 - 822 2.1e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17675
fragaria_vesca FvH4_4g34040 FvH4_5g01010 FvH4_5g01020 FvH4_5g01030 FvH4_5g01040 FvH4_6g39940
malus_domestica MD04G1075600.v1.1 MD04G1075800.v1.1 MD04G1075900.v1.1 MD06G1068300.v1.1 MD12G1007700.v1.1 MD12G1007800.v1.1 MD12G1007900.v1.1 MD14G1005400.v1.1
prunus_persica Prupe.5G084000_v2.0.a1 Prupe.5G084100_v2.0.a1 Prupe.5G084200_v2.0.a1 Prupe.5G084400_v2.0.a1 Prupe.7G008700_v2.0.a1 Prupe.7G009100_v2.0.a1
pyrus_communis pycom04g06640 pycom04g06650 pycom06g06620
rosa_chinensis RchiOBHm_Chr2g0154451 RchiOBHm_Chr2g0154471 RchiOBHm_Chr4g0443531 RchiOBHm_Chr7g0200311 RchiOBHm_Chr7g0200321 RchiOBHm_Chr7g0200341 RchiOBHm_Chr7g0200371
rosa_laevigata RLG00000003732 RLG00000003733 RLG00000003734 RLG00000003735 RLG00000003736 RLG00000003737 RLG00000005919 RLG00000020779
rosa_multiflora Rmu_co8434485.1_g000001 Rmu_sc0000648.1_g000005 Rmu_sc0001118.1_g000024 Rmu_sc0003808.1_g000012 Rmu_sc0005350.1_g000004 Rmu_sc0005350.1_g000005 Rmu_sc0005350.1_g000009 Rmu_sc0005350.1_g000010 Rmu_sc0005350.1_g000011 Rmu_sc0006475.1_g000003
rosa_roxburghii Rroxscaffold_2G00094310 Rroxscaffold_2G00094320 Rroxscaffold_3G00256060 Rroxscaffold_3G00256070 Rroxscaffold_3G00256090 Rroxscaffold_3G00256100 Rroxscaffold_3G00256110 Rroxscaffold_3G00256150 Rroxscaffold_3G00256160 Rroxscaffold_5G00384080
rosa_rugosa Rorug02G0444500 Rorug02G0444600 Rorug04G0347000 Rorug07G0059100 Rorug07G0059200 Rorug07G0059300 Rorug07G0059400 Rorug07G0059500 Rorug07G0059600 Rorug07G0059700 Rorug07G0059800
rosa_samantha Rh2AG509000 Rh2AG509100 Rh2BG520300 Rh2BG520400 Rh2CG494400 Rh2CG494500 Rh2DG530600 Rh2DG530700 Rh4AG402700 Rh4BG414400 Rh4CG430000 Rh4DG409900 Rh4DG411200 Rh7AG184500 Rh7AG184600 Rh7AG184700 Rh7AG184800 Rh7AG184900 Rh7AG185000 Rh7BG187200 Rh7BG187300 Rh7BG187400 Rh7BG187800 Rh7BG187900 Rh7BG188000 Rh7BG188100 Rh7CG194900 Rh7CG195000 Rh7CG195100 Rh7CG195200 Rh7CG195300 Rh7DG188500 Rh7DG188600 Rh7DG188700 Rh7DG188800 Rh7DG188900 Rh7DG189100
rosa_wichuraiana Rw2G041800 Rw2G041810 Rw4G034720 Rw7G016160 Rw7G016170 Rw7G016180 Rw7G016190 Rw7G016200 Rw7G016210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 2717
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1846
Acc36I ACCTGC 5 cut(s) 239, 778, 900, 2222, 2717
AccBSI CCGCTC 1 cut(s) 1972
AccI GTMKAC 5 cut(s) 252, 408, 519, 991, 2678
AccII CGCG 2 cut(s) 27, 192
AciI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
AclWI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
AcoI YGGCCR 1 cut(s) 363
AcsI RAATTY 3 cut(s) 836, 1796, 2113
AcuI CTGAAG 4 cut(s) 981, 1746, 1960, 2435
AcyI GRCGYC 3 cut(s) 126, 198, 1843
AfaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2196
AfiI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
AflIII ACRYGT 2 cut(s) 161, 1687
AhdI GACNNNNNGTC 1 cut(s) 1996
AhlI ACTAGT 1 cut(s) 1640
AjiI CACGTC 1 cut(s) 1199
AjnI CCWGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
AjuI GAANNNNNNNTTGG 2 cut(s) 2616, 2648
AleI CACNNNNGTG 1 cut(s) 1304
Alw21I GWGCWC 2 cut(s) 1526, 2103
Alw26I GTCTC 3 cut(s) 290, 750, 1678
AlwI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
Ama87I CYCGRG 1 cut(s) 2066
AoxI GGCC 3 cut(s) 363, 1265, 1296
ApeKI GCWGC 8 cut(s) 48, 319, 368, 1309, 1631, 2228, 2319, 2723
ApoI RAATTY 3 cut(s) 836, 1796, 2113
ArsI GACNNNNNNTTYG 2 cut(s) 2028, 2060
Asp700I GAANNNNTTC 2 cut(s) 966, 2870
AspLEI GCGC 3 cut(s) 53, 208, 1326
AspS9I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
AxyI CCTNAGG 1 cut(s) 2757
BaeGI GKGCMC 1 cut(s) 334
BamHI GGATCC 1 cut(s) 2312
BanII GRGCYC 1 cut(s) 221
BauI CACGAG 1 cut(s) 1422
BbsI GAAGAC 3 cut(s) 1014, 1020, 1763
Bbv12I GWGCWC 2 cut(s) 1526, 2103
BbvI GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2215, 2306, 2735
BccI CCATC 7 cut(s) 377, 503, 617, 1367, 1438, 1697, 2869
BceAI ACGGC 4 cut(s) 46, 122, 211, 219
BciT130I CCWGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
BcoDI GTCTC 3 cut(s) 290, 750, 1678
BcuI ACTAGT 1 cut(s) 1640
BfaI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2144
BfmI CTRYAG 2 cut(s) 513, 2229
BfoI RGCGCY 3 cut(s) 54, 209, 1327
BfuAI ACCTGC 5 cut(s) 239, 778, 900, 2222, 2717
BisI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2229, 2320, 2724
BlsI GCNGC 9 cut(s) 50, 321, 367, 370, 1311, 1633, 2230, 2321, 2725
Bme1390I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
Bme18I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
BmeRI GACNNNNNGTC 1 cut(s) 1996
BmeT110I CYCGRG 1 cut(s) 2066
BmgBI CACGTC 1 cut(s) 1199
BmgT120I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
BmiI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
BmrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
BmrI ACTGGG 2 cut(s) 516, 532
BmsI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
BmuI ACTGGG 2 cut(s) 516, 532
BoxI GACNNNNGTC 2 cut(s) 1020, 1161
BpiI GAAGAC 3 cut(s) 1014, 1020, 1763
BpmI CTGGAG 1 cut(s) 634
Bpu10I CCTNAGC 1 cut(s) 460
BpuEI CTTGAG 2 cut(s) 2268, 2690
Bsa29I ATCGAT 1 cut(s) 498
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 807, 1658
BsaHI GRCGYC 3 cut(s) 126, 198, 1843
BsaI GGTCTC 1 cut(s) 750
BsaJI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BsaXI ACNNNNNCTCC 2 cut(s) 2447, 2477
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 4 cut(s) 511, 527, 2096, 2244
Bse21I CCTNAGG 1 cut(s) 2757
Bse3DI GCAATG 2 cut(s) 545, 2486
Bse8I GATNNNNATC 2 cut(s) 807, 1658
BseBI CCWGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BseGI GGATG 8 cut(s) 491, 636, 808, 1150, 1741, 2132, 2230, 2525
BseJI GATNNNNATC 2 cut(s) 807, 1658
BseLI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BseMI GCAATG 2 cut(s) 545, 2486
BseMII CTCAG 5 cut(s) 599, 678, 1118, 1922, 2679
BseNI ACTGG 4 cut(s) 511, 527, 2096, 2244
BseRI GAGGAG 4 cut(s) 106, 109, 167, 1091
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2215, 2306, 2735
BseYI CCCAGC 1 cut(s) 1551
BsgI GTGCAG 2 cut(s) 1328, 2727
Bsh1236I CGCG 2 cut(s) 27, 192
BshFI GGCC 3 cut(s) 365, 1267, 1298
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 2 cut(s) 1526, 2103
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 5 cut(s) 10, 54, 243, 456, 1096
BslFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
BslI CCNNNNNNNGG 7 cut(s) 40, 81, 455, 578, 741, 1505, 1838
BsmAI GTCTC 3 cut(s) 290, 750, 1678
BsmFI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
BsmI GAATGC 1 cut(s) 2035
BsnI GGCC 3 cut(s) 365, 1267, 1298
Bso31I GGTCTC 1 cut(s) 750
BsoBI CYCGRG 1 cut(s) 2066
Bsp1286I GDGCHC 4 cut(s) 221, 334, 1526, 2103
Bsp19I CCATGG 1 cut(s) 2323
BspACI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
BspANI GGCC 3 cut(s) 365, 1267, 1298
BspCNI CTCAG 5 cut(s) 600, 679, 1119, 1923, 2680
BspDI ATCGAT 1 cut(s) 498
BspFNI CGCG 2 cut(s) 27, 192
BspHI TCATGA 1 cut(s) 1402
BspLI GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
BspMAI CTGCAG 1 cut(s) 2233
BspMI ACCTGC 5 cut(s) 239, 778, 900, 2222, 2717
BspPI GGATC 5 cut(s) 25, 464, 1648, 2307, 2320
BspQI GCTCTTC 1 cut(s) 1979
BspTNI GGTCTC 1 cut(s) 750
BsrBI CCGCTC 1 cut(s) 1972
BsrDI GCAATG 2 cut(s) 545, 2486
BsrFI RCCGGY 1 cut(s) 455
BsrI ACTGG 4 cut(s) 511, 527, 2096, 2244
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 6 cut(s) 735, 1292, 1414, 1920, 2323, 2370
BssNAI GTATAC 1 cut(s) 520
BssNI GRCGYC 3 cut(s) 126, 198, 1843
BssSI CACGAG 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 735, 1920, 2323
Bst1107I GTATAC 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 1422
Bst2UI CCWGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
Bst4CI ACNGT 9 cut(s) 406, 413, 514, 723, 1254, 1769, 2500, 2769, 2810
Bst6I CTCTTC 1 cut(s) 1979
BstACI GRCGYC 3 cut(s) 126, 198, 1843
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
BstDSI CCRYGG 1 cut(s) 2323
BstENI CCTNNNNNAGG 1 cut(s) 1836
BstF5I GGATG 8 cut(s) 491, 636, 808, 1150, 1741, 2132, 2230, 2525
BstFNI CGCG 2 cut(s) 27, 192
BstH2I RGCGCY 3 cut(s) 54, 209, 1327
BstHHI GCGC 3 cut(s) 53, 208, 1326
BstMAI GTCTC 3 cut(s) 290, 750, 1678
BstMWI GCNNNNNNNGC 5 cut(s) 50, 134, 189, 1273, 1332
BstNI CCWGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
BstNSI RCATGY 4 cut(s) 165, 1345, 1691, 2188
BstPAI GACNNNNGTC 2 cut(s) 1020, 1161
BstSCI CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
BstSFI CTRYAG 2 cut(s) 513, 2229
BstSLI GKGCMC 1 cut(s) 334
BstUI CGCG 2 cut(s) 27, 192
BstV1I GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2215, 2306, 2735
BstV2I GAAGAC 3 cut(s) 1014, 1020, 1763
BstX2I RGATCY 2 cut(s) 30, 2312
BstYI RGATCY 2 cut(s) 30, 2312
BstZ17I GTATAC 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 2757
BsuRI GGCC 3 cut(s) 365, 1267, 1298
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 2323
BtgZI GCGATG 1 cut(s) 2759
BtrI CACGTC 1 cut(s) 1199
BtsCI GGATG 8 cut(s) 491, 636, 808, 1150, 1741, 2132, 2230, 2525
BtsI GCAGTG 2 cut(s) 681, 936
BtsIMutI CAGTG 7 cut(s) 681, 936, 957, 1218, 1466, 1984, 2103
BveI ACCTGC 5 cut(s) 239, 778, 900, 2222, 2717
Cac8I GCNNGC 6 cut(s) 46, 210, 830, 1112, 1743, 1829
CciI TCATGA 1 cut(s) 1402
CfoI GCGC 3 cut(s) 53, 208, 1326
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
ClaI ATCGAT 1 cut(s) 498
CseI GACGC 2 cut(s) 134, 187
Csp6I GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
CspCI CAANNNNNGTGG 4 cut(s) 1608, 1643, 2067, 2102
CviQI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
DraI TTTAAA 1 cut(s) 2269
DrdI GACNNNNNNGTC 1 cut(s) 260
DriI GACNNNNNGTC 1 cut(s) 1996
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 1979
Eam1105I GACNNNNNGTC 1 cut(s) 1996
EarI CTCTTC 1 cut(s) 1979
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 735, 1920, 2323
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 750
Eco47I GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
Eco57I CTGAAG 4 cut(s) 981, 1746, 1960, 2435
Eco81I CCTNAGG 1 cut(s) 2757
Eco88I CYCGRG 1 cut(s) 2066
EcoNI CCTNNNNNAGG 1 cut(s) 1836
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
EcoT14I CCWWGG 3 cut(s) 735, 1920, 2323
EcoT22I ATGCAT 3 cut(s) 2170, 2190, 2524
EcoT38I GRGCYC 1 cut(s) 221
ErhI CCWWGG 3 cut(s) 735, 1920, 2323
FalI AAGNNNNNCTT 2 cut(s) 50, 82
FaqI GGGAC 5 cut(s) 98, 367, 523, 1153, 2486
FauI CCCGC 1 cut(s) 1977
FauNDI CATATG 1 cut(s) 1072
FblI GTMKAC 5 cut(s) 252, 408, 519, 991, 2678
Fnu4HI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2229, 2320, 2724
FokI GGATG 8 cut(s) 478, 643, 815, 1157, 1748, 2119, 2237, 2512
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2229, 2320, 2724
FspBI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2144
GlaI GCGC 3 cut(s) 52, 207, 1325
GluI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2229, 2320, 2724
GsaI CCCAGC 1 cut(s) 1555
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 3 cut(s) 54, 209, 1327
HaeIII GGCC 3 cut(s) 365, 1267, 1298
HapII CCGG 5 cut(s) 10, 54, 243, 456, 1096
HgaI GACGC 2 cut(s) 134, 187
HhaI GCGC 3 cut(s) 53, 208, 1326
Hin1I GRCGYC 3 cut(s) 126, 198, 1843
Hin6I GCGC 3 cut(s) 51, 206, 1324
HinP1I GCGC 3 cut(s) 51, 206, 1324
HincII GTYRAC 2 cut(s) 253, 2679
HindII GTYRAC 2 cut(s) 253, 2679
HindIII AAGCTT 2 cut(s) 2262, 2333
HpaII CCGG 5 cut(s) 10, 54, 243, 456, 1096
Hpy166II GTNNAC 9 cut(s) 142, 253, 409, 520, 713, 992, 1421, 2249, 2679
Hpy188III TCNNGA 8 cut(s) 537, 620, 1025, 1403, 2285, 2669, 2813, 2878
Hpy8I GTNNAC 9 cut(s) 142, 253, 409, 520, 713, 992, 1421, 2249, 2679
Hpy99I CGWCG 7 cut(s) 80, 128, 197, 254, 1203, 2683, 2686
HpyAV CCTTC 7 cut(s) 76, 948, 1580, 1594, 1889, 2734, 2821
HpyCH4III ACNGT 9 cut(s) 406, 413, 514, 723, 1254, 1769, 2500, 2769, 2810
HpyCH4IV ACGT 4 cut(s) 328, 1198, 1843, 2597
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 134, 189, 1273, 1332
HpySE526I ACGT 4 cut(s) 328, 1198, 1843, 2597
Hsp92I GRCGYC 3 cut(s) 126, 198, 1843
HspAI GCGC 3 cut(s) 51, 206, 1324
LguI GCTCTTC 1 cut(s) 1979
LmnI GCTCC 8 cut(s) 77, 180, 251, 574, 1349, 1913, 2147, 2596
Lsp1109I GCAGC 8 cut(s) 60, 306, 355, 1321, 1643, 2215, 2306, 2735
LweI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
MaeI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2144
MaeII ACGT 4 cut(s) 328, 1198, 1843, 2597
MaeIII GTNAC 8 cut(s) 106, 199, 451, 1714, 1750, 2404, 2729, 2769
MbiI CCGCTC 1 cut(s) 1972
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 30, 2312
MhlI GDGCHC 4 cut(s) 221, 334, 1526, 2103
MlyI GAGTC 5 cut(s) 625, 1088, 1115, 1849, 1910
MmeI TCCRAC 3 cut(s) 147, 1181, 2614
Mph1103I ATGCAT 3 cut(s) 2170, 2190, 2524
MroXI GAANNNNTTC 2 cut(s) 966, 2870
MslI CAYNNNNRTG 2 cut(s) 1304, 1529
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 5 cut(s) 10, 54, 243, 456, 1096
MspR9I CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 2035
MvaI CCWGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
MvnI CGCG 2 cut(s) 27, 192
MwoI GCNNNNNNNGC 5 cut(s) 50, 134, 189, 1273, 1332
NcoI CCATGG 1 cut(s) 2323
NdeI CATATG 1 cut(s) 1072
NlaIV GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
NmeAIII GCCGAG 1 cut(s) 1439
NmuCI GTSAC 6 cut(s) 106, 199, 1714, 1750, 2729, 2769
NsiI ATGCAT 3 cut(s) 2170, 2190, 2524
NspI RCATGY 4 cut(s) 165, 1345, 1691, 2188
OliI CACNNNNGTG 1 cut(s) 1304
PagI TCATGA 1 cut(s) 1402
PaqCI CACCTGC 2 cut(s) 239, 2717
PciI ACATGT 2 cut(s) 161, 1687
PciSI GCTCTTC 1 cut(s) 1979
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PctI GAATGC 1 cut(s) 2035
PdmI GAANNNNTTC 2 cut(s) 966, 2870
PfeI GAWTC 8 cut(s) 312, 500, 934, 973, 1181, 1204, 2200, 2866
PflFI GACNNNGTC 2 cut(s) 197, 881
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 9 cut(s) 50, 321, 367, 370, 1311, 1633, 2230, 2321, 2725
PleI GAGTC 5 cut(s) 624, 1087, 1115, 1849, 1910
PpsI GAGTC 5 cut(s) 624, 1087, 1115, 1849, 1910
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 2 cut(s) 161, 1687
PshAI GACNNNNGTC 2 cut(s) 1020, 1161
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
PspFI CCCAGC 1 cut(s) 1551
PspGI CCWGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
PspN4I GGNNCC 5 cut(s) 86, 247, 1297, 1967, 2314
PspPI GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
PspPPI RGGWCCY 1 cut(s) 446
PstI CTGCAG 1 cut(s) 2233
PsuI RGATCY 2 cut(s) 30, 2312
PsyI GACNNNGTC 2 cut(s) 197, 881
RsaI GTAC 7 cut(s) 415, 606, 1036, 1379, 1618, 1806, 2196
RsaNI GTAC 7 cut(s) 414, 605, 1035, 1378, 1617, 1805, 2195
RseI CAYNNNNRTG 2 cut(s) 1304, 1529
SalI GTCGAC 2 cut(s) 251, 2677
SapI GCTCTTC 1 cut(s) 1979
SatI GCNGC 9 cut(s) 49, 320, 366, 369, 1310, 1632, 2229, 2320, 2724
Sau96I GGNCC 6 cut(s) 7, 85, 446, 1296, 1418, 1706
SchI GAGTC 5 cut(s) 625, 1088, 1115, 1849, 1910
ScrFI CCNGG 6 cut(s) 645, 1264, 1293, 1314, 1860, 2372
SduI GDGCHC 4 cut(s) 221, 334, 1526, 2103
SfaNI GCATC 8 cut(s) 500, 1668, 1730, 2141, 2197, 2215, 2371, 2531
SfcI CTRYAG 2 cut(s) 513, 2229
SinI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
SmiMI CAYNNNNRTG 2 cut(s) 1304, 1529
SmlI CTYRAG 2 cut(s) 2283, 2669
SmoI CTYRAG 2 cut(s) 2283, 2669
SpeI ACTAGT 1 cut(s) 1640
SsiI CCGC 9 cut(s) 25, 111, 167, 181, 190, 366, 1657, 1970, 2857
SspI AATATT 1 cut(s) 304
SspMI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2144
StyD4I CCNGG 6 cut(s) 643, 1262, 1291, 1312, 1858, 2370
StyI CCWWGG 3 cut(s) 735, 1920, 2323
TaaI ACNGT 9 cut(s) 406, 413, 514, 723, 1254, 1769, 2500, 2769, 2810
TaiI ACGT 4 cut(s) 331, 1201, 1846, 2600
TaqI TCGA 5 cut(s) 252, 498, 1701, 2678, 2864
TaqII GACCGA 1 cut(s) 2875
TatI WGTACW 2 cut(s) 604, 1034
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 8 cut(s) 312, 500, 934, 973, 1181, 1204, 2200, 2866
TscAI CASTG 7 cut(s) 688, 936, 964, 1225, 1473, 1984, 2103
TseFI GTSAC 6 cut(s) 106, 199, 1714, 1750, 2729, 2769
TseI GCWGC 8 cut(s) 48, 319, 368, 1309, 1631, 2228, 2319, 2723
Tsp45I GTSAC 6 cut(s) 106, 199, 1714, 1750, 2729, 2769
TspGWI ACGGA 6 cut(s) 64, 94, 238, 250, 643, 2859
TspRI CASTG 7 cut(s) 688, 936, 964, 1225, 1473, 1984, 2103
Tth111I GACNNNGTC 2 cut(s) 197, 881
VpaK11BI GGWCC 5 cut(s) 7, 85, 446, 1418, 1706
XagI CCTNNNNNAGG 1 cut(s) 1836
XapI RAATTY 3 cut(s) 836, 1796, 2113
XceI RCATGY 4 cut(s) 165, 1345, 1691, 2188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1548
XmiI GTMKAC 5 cut(s) 252, 408, 519, 991, 2678
XmnI GAANNNNTTC 2 cut(s) 966, 2870
XspI CTAG 6 cut(s) 282, 887, 1092, 1641, 1983, 2144
ZraI GACGTC 1 cut(s) 1844
Zsp2I ATGCAT 3 cut(s) 2170, 2190, 2524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.