Rorug07G0059600

Blue copper

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000318
Physical Location & Seq
Reverse (-)
4271491 .. 4275687
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0059600.1

Sequence Viewer

Length: 2901 bp
ATGGCCTCTGTGGCACCACCACCACTCCCATTCTCATATCATCATCATCATCATCCCCTGCTTCCCAAACCGAACCCCAAACACTCCATTCTCACACCAAAATCTTCACTTTCTTTCTCTCCCACTCAAACCACCTCTCTCCCTCCAACCAAAACCAACTCACTCCAAGACCTCAACCACCTCTGCAACTCCGGCAACCTCTCCGCCGCCCTCACCCTCCTCCAAACCAATGCCTTCCCCACCTCACAGCACCTCAAAGACGCCATCGGCGCCCTCCTCCAGGCCTGTGGCCGCCACAAGGACATCGAAACCGGCCGCAAACTCCACCACCTCGTCTCCCAATCCACCCACTTCATCCACGACTTCGTCCTCAACACCCGCATCATCACCATGTACTCAATGTGCAACTCTCCTTCCGATTCCCGCCTCGTTTTCGAAGGGTTACAGAGGAGGAACTTGTTCCAGTGGAATGCTCTGGTTAGTGGGTACTCAAGAAACGAGCTTTATGAGGAAGCAATTGGTGCCTTTATTAAGTTGATAAGCGCCGCGGAGTTTAAACCCGATAATTTCACAATGCCTTGTGTGATCAAGGCCTGTGGTGGGCTTTTGGATGTGGGTTTGGGGCAGGGGGTTCATGGGATGGTTGTGAAGATGGGTTTGGTTTCGGATGTGTTTGTGGGGAATGCATTGATTGCGGTGTACGGCAAATTTGGGTTTGTTGGGGATTCAGTTAAGGTGTTTGAGAAAATGAGTGCGAGGAACTTGGTGTCTTGGAATTCGATGATTGGGGTGTTTTCGGAGAATGGGATGTGTGAAGAGAGTTGTAGGTTGTTGGTGAGGTTTTTGGAGGGTGAGGAAGGGTTTGTTCCGGATGAGGCGACGTTGGTGACTATATTACCTGTTTGTGCTGGGAAAGGGGATGTTAGAATGGGGATGGAGATTCATGGTTTGGCGGTGAAGTTGGGGCTGGATAAGGAGTTGATGTTGAGTAATGCCGTGATGGGCATGTATTTGAAATGTGGGTACTTGACGGAAGCTCGAGTTTCGTTTGAGAGAAATGAGAGGAAGAATGTGGTGTCTTGGAATGCTTTCGTAGGAGGTTATTCTAGACAAGGGGATGTTAGTGGAGCATTTGATCTTTTGCGAAAAATGCAGATGAAGGAGAAGGTGAATGAGGTCACCGTCTTAAATGTGTTACCGGTTTTTTTAGAGGAGTCGGAACTTTTGAGCTTGAAAGAGCTTCATGGTTACTCATTCAGACATGGGTTTCAAGATGATGAGCTGGTAGCCAATGCTTTTGTTGCAGCCTATGCGAAGTGTGGGTCGCTGAGTTCTGCTGAGCAGGTGTTTTATGGTATTGAGACGAAGACAGTGAGCTCCTGGAATGCAGTTATTGGTGGCCATGCCCAGAATGGAGATCCACAAAAGGCTTTAGATTTGTACCTTCAAATGAAACAATCAGGTTTGGATCCTGACAAGTTTAGCATCGGTAGCCTCCTTTTAGCTTGTGCCCACCTCAAATTTCTTCAATATGGTAAAGAGATTCATGGGTTTGTGCTACGTAATGGGTTAGAGTTGGATTCATTTATTGGTATTTCATTACTATCCTTTTACATCCAATGTGGTAAACTATCGAAGGCAAGAGCATTATTTGATAGGATGGAAGATAAAATTCCAGTGTCATGGAATGCAATAATCTCTGGTTACTCTCAGATTGGACTTCCTGATGAAGCCCTCGATCTCTTTCGACTAATGCTGTCTAATGGAATCCTACCTGGTGAAATAGCCACAATGAGTGCACTGGGGGCTTGTTCACAACTGTCAGCTCTGCGTCTAGGGAAAGAACTGCATTGTTTTGCTTTGAAAGCACGCCTAACAGAAGACCTGTTTGTTGGTTGTTCTCTCATAGATATGTATGCAAAAAGTGGCTGCATAGAACAATCTTACAGGGCATTTGACAGTTTAACAAAGAAAGATGTGGCATCATGGAATGTTATAATTGCAGGGTATGGAATTCATGGACATGGAAACAAGGCTTTAGAGCTATTTGGAGAAATGATAAGTTTGGGGCATAAGCCTGATGTCTTTACATTTCTTGGAATTCTAACTGCATGTAACCATGCTGGGCTTGTTAAAAATGGGCTAAAATATTTCAAAGAGATGCAAAGATTATATGGAATAGAGCCGAAGTTAGAGCATTATGCATGTGTAGTAGACATGCTGGGTAGGGCTGGTCAATTGGAGGAAGCTTTAAACCTGATAGATAGCATGCCTGAAGAACCAGATACTAGAATTTGGAGTTCATTGCTTAGTTCCTGTAGGAATTATAATGATTTGGGAATAGGACAGAAAATTGCTGAAAAATTACTAGAATTGGAGCCCGGAAAGGCAGAGAATTATGTCCTACTGTCGAACTTATATGCTGCATCAGGGAATTGGGATGATGTGAGATGGGTCCGGCAAAGGATGAAGGAGATTGGCCTTCAGAAAGAAGCTGGCCGCAGTTGGATTGAAATTGGAGGGCAAGTGTATAACTTTGTTGCTGGTGACAATTCTCTGCCAGAGTCGGCGGAGATTAGGAAGATGTGGACAAAAGTAGAGGAAAGAATAAGTAAACTCGGATATACACCCAACACAGATTCTGTGCTTCATGAACTGGACTATACAGAGAAGATTGAAATATTGAGGGGGCATAGTGAAAAGCTTGCAATTTCATTTGGATTGTTAAAGATGAATAAAGGTGCTACACTGAGAGTCTGTAAAAATCTGCGCATCTGCATTGATTGTCACAATGCTGCTAAGTTGATATCAAAGGCTGTTGAAACGGAGATAATTGTGAGAGACAACAAGCGCTTCCATCATTTCAAAGATGGCCTCTGTTCTTGTGGAGATTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

966

Amino Acids

107.05

Weight (kDa)

6.69

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 457 - 505 3.4e-10 PPR repeat family
PPR PF01535 460 - 489 3.9e-07 PPR repeat
PPR PF01535 560 - 590 7.5e-09 PPR repeat
PPR_2 PF13041 560 - 594 2.4e-06 PPR repeat family
PPR_2 PF13041 658 - 705 4.6e-11 PPR repeat family
PPR PF01535 662 - 690 2.6e-06 PPR repeat
E_motif PF20431 776 - 838 8.2e-18 E motif
DYW_deaminase PF14432 874 - 966 2.7e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000318)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17675
fragaria_vesca FvH4_4g34040 FvH4_5g01010 FvH4_5g01020 FvH4_5g01030 FvH4_5g01040 FvH4_6g39940
malus_domestica MD04G1075600.v1.1 MD04G1075800.v1.1 MD04G1075900.v1.1 MD06G1068300.v1.1 MD12G1007700.v1.1 MD12G1007800.v1.1 MD12G1007900.v1.1 MD14G1005400.v1.1
prunus_persica Prupe.5G084000_v2.0.a1 Prupe.5G084100_v2.0.a1 Prupe.5G084200_v2.0.a1 Prupe.5G084400_v2.0.a1 Prupe.7G008700_v2.0.a1 Prupe.7G009100_v2.0.a1
pyrus_communis pycom04g06640 pycom04g06650 pycom06g06620
rosa_chinensis RchiOBHm_Chr2g0154451 RchiOBHm_Chr2g0154471 RchiOBHm_Chr4g0443531 RchiOBHm_Chr7g0200311 RchiOBHm_Chr7g0200321 RchiOBHm_Chr7g0200341 RchiOBHm_Chr7g0200371
rosa_laevigata RLG00000003732 RLG00000003733 RLG00000003734 RLG00000003735 RLG00000003736 RLG00000003737 RLG00000005919 RLG00000020779
rosa_multiflora Rmu_co8434485.1_g000001 Rmu_sc0000648.1_g000005 Rmu_sc0001118.1_g000024 Rmu_sc0003808.1_g000012 Rmu_sc0005350.1_g000004 Rmu_sc0005350.1_g000005 Rmu_sc0005350.1_g000009 Rmu_sc0005350.1_g000010 Rmu_sc0005350.1_g000011 Rmu_sc0006475.1_g000003
rosa_roxburghii Rroxscaffold_2G00094310 Rroxscaffold_2G00094320 Rroxscaffold_3G00256060 Rroxscaffold_3G00256070 Rroxscaffold_3G00256090 Rroxscaffold_3G00256100 Rroxscaffold_3G00256110 Rroxscaffold_3G00256150 Rroxscaffold_3G00256160 Rroxscaffold_5G00384080
rosa_rugosa Rorug02G0444500 Rorug02G0444600 Rorug04G0347000 Rorug07G0059100 Rorug07G0059200 Rorug07G0059300 Rorug07G0059400 Rorug07G0059500 Rorug07G0059600 Rorug07G0059700 Rorug07G0059800
rosa_samantha Rh2AG509000 Rh2AG509100 Rh2BG520300 Rh2BG520400 Rh2CG494400 Rh2CG494500 Rh2DG530600 Rh2DG530700 Rh4AG402700 Rh4BG414400 Rh4CG430000 Rh4DG409900 Rh4DG411200 Rh7AG184500 Rh7AG184600 Rh7AG184700 Rh7AG184800 Rh7AG184900 Rh7AG185000 Rh7BG187200 Rh7BG187300 Rh7BG187400 Rh7BG187800 Rh7BG187900 Rh7BG188000 Rh7BG188100 Rh7CG194900 Rh7CG195000 Rh7CG195100 Rh7CG195200 Rh7CG195300 Rh7DG188500 Rh7DG188600 Rh7DG188700 Rh7DG188800 Rh7DG188900 Rh7DG189100
rosa_wichuraiana Rw2G041800 Rw2G041810 Rw4G034720 Rw7G016160 Rw7G016170 Rw7G016180 Rw7G016190 Rw7G016200 Rw7G016210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1997, 2328
AarI CACCTGC 1 cut(s) 1333
Acc16I TGCGCA 1 cut(s) 2771
Acc36I ACCTGC 1 cut(s) 1333
AccB1I GGYRCC 3 cut(s) 13, 269, 521
AccI GTMKAC 1 cut(s) 2214
AccII CGCG 1 cut(s) 548
AccIII TCCGGA 1 cut(s) 868
AclWI GGATC 3 cut(s) 1412, 1463, 1476
AcoI YGGCCR 4 cut(s) 289, 313, 1399, 2497
AcsI RAATTY 7 cut(s) 707, 775, 1520, 1671, 2013, 2100, 2292
AcuI CTGAAG 2 cut(s) 2295, 2468
AcyI GRCGYC 2 cut(s) 261, 270
AfaI GTAC 5 cut(s) 395, 488, 701, 1025, 1442
AfeI AGCGCT 1 cut(s) 2852
AfiI CCNNNNNNNGG 3 cut(s) 280, 298, 600
AgeI ACCGGT 1 cut(s) 1198
AjnI CCWGG 3 cut(s) 279, 1379, 1774
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
Alw21I GWGCWC 2 cut(s) 1379, 1801
Alw26I GTCTC 3 cut(s) 340, 1355, 2835
Alw44I GTGCAC 1 cut(s) 1797
AlwI GGATC 3 cut(s) 1412, 1463, 1476
AlwNI CAGNNNCTG 1 cut(s) 2642
Ama87I CYCGRG 1 cut(s) 1038
Aor13HI TCCGGA 1 cut(s) 868
Aor51HI AGCGCT 1 cut(s) 2852
AoxI GGCC 9 cut(s) 3, 282, 289, 313, 591, 1399, 2479, 2497, 2872
ApaLI GTGCAC 1 cut(s) 1797
ApeKI GCWGC 4 cut(s) 1304, 1929, 2423, 2795
ApoI RAATTY 7 cut(s) 707, 775, 1520, 1671, 2013, 2100, 2292
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
AsiGI ACCGGT 1 cut(s) 1198
Asp700I GAANNNNTTC 2 cut(s) 458, 1088
AspLEI GCGC 4 cut(s) 272, 545, 2772, 2853
AspS9I GGNCC 1 cut(s) 2455
AsuC2I CCSGG 1 cut(s) 2382
AsuII TTCGAA 1 cut(s) 435
AvaI CYCGRG 1 cut(s) 1038
AvaII GGWCC 1 cut(s) 2455
BaeGI GKGCMC 2 cut(s) 1513, 1801
BalI TGGCCA 1 cut(s) 1401
BamHI GGATCC 1 cut(s) 1468
BanI GGYRCC 3 cut(s) 13, 269, 521
BanII GRGCYC 2 cut(s) 1379, 2382
BbsI GAAGAC 2 cut(s) 1373, 1887
Bbv12I GWGCWC 2 cut(s) 1379, 1801
BbvI GCAGC 4 cut(s) 1316, 1916, 2410, 2782
BccI CCATC 9 cut(s) 272, 634, 646, 928, 994, 1654, 2445, 2864, 2865
BceAI ACGGC 2 cut(s) 718, 980
BciT130I CCWGG 3 cut(s) 281, 1381, 1776
BclI TGATCA 1 cut(s) 585
BcnI CCSGG 1 cut(s) 2382
BcoDI GTCTC 3 cut(s) 340, 1355, 2835
BfaI CTAG 4 cut(s) 1107, 1835, 2289, 2369
BfmI CTRYAG 1 cut(s) 2317
BfoI RGCGCY 3 cut(s) 273, 546, 2854
BfuAI ACCTGC 1 cut(s) 1333
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 9 cut(s) 207, 292, 316, 546, 1305, 1930, 2424, 2500, 2796
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 9 cut(s) 208, 293, 317, 547, 1306, 1931, 2425, 2501, 2797
Bme1390I CCNGG 4 cut(s) 281, 1381, 1776, 2382
Bme18I GGWCC 1 cut(s) 2455
BmeT110I CYCGRG 1 cut(s) 1038
BmgT120I GGNCC 1 cut(s) 2455
BmiI GGNNCC 6 cut(s) 15, 271, 523, 1470, 2379, 2456
BmrFI CCNGG 4 cut(s) 281, 1381, 1776, 2382
BmrI ACTGGG 1 cut(s) 1811
BmsI GCATC 6 cut(s) 390, 1494, 1991, 2151, 2435, 2781
BmuI ACTGGG 1 cut(s) 1811
BpiI GAAGAC 2 cut(s) 1373, 1887
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 1338
Bpu14I TTCGAA 1 cut(s) 435
BpuEI CTTGAG 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 2382
BsaAI YACGTR 1 cut(s) 1562
BsaHI GRCGYC 2 cut(s) 261, 270
BsaJI CCNNGG 1 cut(s) 546
BsaWI WCCGGW 2 cut(s) 868, 1198
BsaXI ACNNNNNCTCC 8 cut(s) 9, 39, 320, 350, 968, 998, 2511, 2541
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 298, 600
Bse118I RCCGGY 2 cut(s) 311, 1198
Bse1I ACTGG 4 cut(s) 463, 1676, 1806, 2661
Bse3DI GCAATG 1 cut(s) 2303
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 3 cut(s) 281, 1381, 1776
BseDI CCNNGG 1 cut(s) 546
BseLI CCNNNNNNNGG 3 cut(s) 280, 298, 600
BseMI GCAATG 1 cut(s) 2303
BseMII CTCAG 4 cut(s) 1319, 1329, 1724, 2741
BseNI ACTGG 4 cut(s) 463, 1676, 1806, 2661
BseRI GAGGAG 4 cut(s) 209, 266, 463, 1226
BseSI GKGCMC 2 cut(s) 1513, 1801
BseX3I CGGCCG 1 cut(s) 313
BseXI GCAGC 4 cut(s) 1316, 1916, 2410, 2782
BseYI CCCAGC 3 cut(s) 908, 2123, 2221
Bsh1236I CGCG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 9 cut(s) 5, 284, 291, 315, 593, 1401, 2481, 2499, 2874
BshNI GGYRCC 3 cut(s) 13, 269, 521
BshTI ACCGGT 1 cut(s) 1198
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 2 cut(s) 1379, 1801
BsiHKCI CYCGRG 1 cut(s) 1038
BsiSI CCGG 6 cut(s) 192, 312, 869, 1199, 2382, 2458
BslI CCNNNNNNNGG 3 cut(s) 280, 298, 600
BsmAI GTCTC 3 cut(s) 340, 1355, 2835
BsmBI CGTCTC 2 cut(s) 340, 1355
BsmI GAATGC 5 cut(s) 475, 688, 1090, 1390, 1693
BsnI GGCC 9 cut(s) 5, 284, 291, 315, 593, 1401, 2481, 2499, 2874
BsoBI CYCGRG 1 cut(s) 1038
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 4 cut(s) 1379, 1513, 1801, 2382
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
Bsp1720I GCTNAGC 1 cut(s) 1338
BspANI GGCC 9 cut(s) 5, 284, 291, 315, 593, 1401, 2481, 2499, 2874
BspCNI CTCAG 4 cut(s) 1320, 1330, 1723, 2742
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 1 cut(s) 548
BspHI TCATGA 1 cut(s) 2650
BspLI GGNNCC 6 cut(s) 15, 271, 523, 1470, 2379, 2456
BspMI ACCTGC 1 cut(s) 1333
BspPI GGATC 3 cut(s) 1412, 1463, 1476
BspT104I TTCGAA 1 cut(s) 435
BspT107I GGYRCC 3 cut(s) 13, 269, 521
BsrDI GCAATG 1 cut(s) 2303
BsrFI RCCGGY 2 cut(s) 311, 1198
BsrI ACTGG 4 cut(s) 463, 1676, 1806, 2661
BssAI RCCGGY 2 cut(s) 311, 1198
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
BssNI GRCGYC 2 cut(s) 261, 270
Bst2UI CCWGG 3 cut(s) 281, 1381, 1776
Bst4CI ACNGT 5 cut(s) 1183, 1372, 1821, 1961, 2409
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 2 cut(s) 261, 270
BstAPI GCANNNNNTGC 3 cut(s) 521, 692, 1310
BstBAI YACGTR 1 cut(s) 1562
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 4 cut(s) 1870, 2270, 2497, 2706
BstDEI CTNAG 6 cut(s) 1328, 1338, 1710, 2309, 2750, 2799
BstDSI CCRYGG 1 cut(s) 546
BstEII GGTNACC 1 cut(s) 1177
BstENI CCTNNNNNAGG 1 cut(s) 278
BstFNI CGCG 1 cut(s) 548
BstH2I RGCGCY 3 cut(s) 273, 546, 2854
BstHHI GCGC 4 cut(s) 272, 545, 2772, 2853
BstKTI GATC 5 cut(s) 588, 1138, 1420, 1471, 1741
BstMAI GTCTC 3 cut(s) 340, 1355, 2835
BstMBI GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
BstMCI CGRYCG 1 cut(s) 316
BstNI CCWGG 3 cut(s) 281, 1381, 1776
BstNSI RCATGY 5 cut(s) 1009, 2115, 2208, 2221, 2272
BstPI GGTNACC 1 cut(s) 1177
BstSCI CCNGG 4 cut(s) 279, 1379, 1774, 2380
BstSFI CTRYAG 1 cut(s) 2317
BstSLI GKGCMC 2 cut(s) 1513, 1801
BstSNI TACGTA 1 cut(s) 1562
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 4 cut(s) 1316, 1916, 2410, 2782
BstV2I GAAGAC 2 cut(s) 1373, 1887
BstX2I RGATCY 2 cut(s) 1417, 1468
BstXI CCANNNNNNTGG 2 cut(s) 287, 1683
BstYI RGATCY 2 cut(s) 1417, 1468
BstZI CGGCCG 1 cut(s) 313
BsuRI GGCC 9 cut(s) 5, 284, 291, 315, 593, 1401, 2481, 2499, 2874
BtgI CCRYGG 1 cut(s) 546
BtsIMutI CAGTG 5 cut(s) 470, 1377, 1683, 1799, 2747
BveI ACCTGC 1 cut(s) 1333
Cac8I GCNNGC 4 cut(s) 1870, 2270, 2497, 2706
CaiI CAGNNNCTG 1 cut(s) 2642
CciI TCATGA 1 cut(s) 2650
CfoI GCGC 4 cut(s) 272, 545, 2772, 2853
Cfr10I RCCGGY 2 cut(s) 311, 1198
Cfr13I GGNCC 1 cut(s) 2455
Cfr42I CCGCGG 1 cut(s) 549
CseI GACGC 2 cut(s) 269, 1820
CsiI ACCWGGT 1 cut(s) 1774
Csp6I GTAC 5 cut(s) 394, 487, 700, 1024, 1441
CspAI ACCGGT 1 cut(s) 1198
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviQI GTAC 5 cut(s) 394, 487, 700, 1024, 1441
DdeI CTNAG 6 cut(s) 1328, 1338, 1710, 2309, 2750, 2799
DinI GGCGCC 1 cut(s) 271
DpnI GATC 5 cut(s) 587, 1137, 1419, 1470, 1740
DpnII GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
DraI TTTAAA 2 cut(s) 556, 2253
EaeI YGGCCR 4 cut(s) 289, 313, 1399, 2497
EagI CGGCCG 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 2 cut(s) 193, 2585
Ecl136II GAGCTC 1 cut(s) 1377
EclXI CGGCCG 1 cut(s) 313
Eco105I TACGTA 1 cut(s) 1562
Eco147I AGGCCT 2 cut(s) 284, 593
Eco24I GRGCYC 2 cut(s) 1379, 2382
Eco32I GATATC 1 cut(s) 2808
Eco47I GGWCC 1 cut(s) 2455
Eco47III AGCGCT 1 cut(s) 2852
Eco52I CGGCCG 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 1377
Eco57I CTGAAG 2 cut(s) 2295, 2468
Eco88I CYCGRG 1 cut(s) 1038
Eco91I GGTNACC 1 cut(s) 1177
EcoICRI GAGCTC 1 cut(s) 1377
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO65I GGTNACC 1 cut(s) 1177
EcoRI GAATTC 3 cut(s) 775, 2013, 2100
EcoRII CCWGG 3 cut(s) 279, 1379, 1774
EcoRV GATATC 1 cut(s) 2808
EcoT22I ATGCAT 2 cut(s) 688, 2206
EcoT38I GRGCYC 2 cut(s) 1379, 2382
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
Esp3I CGTCTC 2 cut(s) 340, 1355
FauI CCCGC 2 cut(s) 386, 431
FbaI TGATCA 1 cut(s) 585
FblI GTMKAC 1 cut(s) 2214
Fnu4HI GCNGC 9 cut(s) 207, 292, 316, 546, 1305, 1930, 2424, 2500, 2796
FriOI GRGCYC 2 cut(s) 1379, 2382
Fsp4HI GCNGC 9 cut(s) 207, 292, 316, 546, 1305, 1930, 2424, 2500, 2796
FspBI CTAG 4 cut(s) 1107, 1835, 2289, 2369
FspI TGCGCA 1 cut(s) 2771
GlaI GCGC 4 cut(s) 271, 544, 2771, 2852
GluI GCNGC 9 cut(s) 207, 292, 316, 546, 1305, 1930, 2424, 2500, 2796
GsaI CCCAGC 3 cut(s) 912, 2127, 2225
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 3 cut(s) 273, 546, 2854
HaeIII GGCC 9 cut(s) 5, 284, 291, 315, 593, 1401, 2481, 2499, 2874
HapII CCGG 6 cut(s) 192, 312, 869, 1199, 2382, 2458
HgaI GACGC 2 cut(s) 269, 1820
HhaI GCGC 4 cut(s) 272, 545, 2772, 2853
Hin1I GRCGYC 2 cut(s) 261, 270
Hin6I GCGC 4 cut(s) 270, 543, 2770, 2851
HinP1I GCGC 4 cut(s) 270, 543, 2770, 2851
HindIII AAGCTT 2 cut(s) 2247, 2702
HpaII CCGG 6 cut(s) 192, 312, 869, 1199, 2382, 2458
Hpy166II GTNNAC 7 cut(s) 700, 1628, 1799, 1814, 2215, 2589, 2615
Hpy188I TCNGA 8 cut(s) 418, 667, 799, 1219, 1259, 1713, 2487, 2621
Hpy188III TCNNGA 7 cut(s) 492, 869, 1107, 1271, 1472, 1724, 2651
Hpy8I GTNNAC 7 cut(s) 700, 1628, 1799, 1814, 2215, 2589, 2615
Hpy99I CGWCG 1 cut(s) 883
HpyCH4III ACNGT 5 cut(s) 1183, 1372, 1821, 1961, 2409
HpyCH4IV ACGT 2 cut(s) 881, 1561
HpyF3I CTNAG 6 cut(s) 1328, 1338, 1710, 2309, 2750, 2799
HpySE526I ACGT 2 cut(s) 881, 1561
Hsp92I GRCGYC 2 cut(s) 261, 270
HspAI GCGC 4 cut(s) 270, 543, 2770, 2851
KasI GGCGCC 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 585
KspI CCGCGG 1 cut(s) 549
Kzo9I GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
LmnI GCTCC 3 cut(s) 1127, 1382, 2377
Lsp1109I GCAGC 4 cut(s) 1316, 1916, 2410, 2782
LweI GCATC 6 cut(s) 390, 1494, 1991, 2151, 2435, 2781
MabI ACCWGGT 1 cut(s) 1774
MaeI CTAG 4 cut(s) 1107, 1835, 2289, 2369
MaeII ACGT 2 cut(s) 881, 1561
MaeIII GTNAC 9 cut(s) 441, 886, 1177, 1194, 1247, 1703, 2114, 2546, 2786
MalI GATC 5 cut(s) 587, 1137, 1419, 1470, 1740
MboI GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
MfeI CAATTG 2 cut(s) 516, 2237
MflI RGATCY 2 cut(s) 1417, 1468
MhlI GDGCHC 4 cut(s) 1379, 1513, 1801, 2382
MlsI TGGCCA 1 cut(s) 1401
MluNI TGGCCA 1 cut(s) 1401
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 3 cut(s) 1223, 2573, 2763
MmeI TCCRAC 4 cut(s) 170, 1197, 1557, 2486
Mox20I TGGCCA 1 cut(s) 1401
Mph1103I ATGCAT 2 cut(s) 688, 2206
MroI TCCGGA 1 cut(s) 868
MroXI GAANNNNTTC 2 cut(s) 458, 1088
MscI TGGCCA 1 cut(s) 1401
MseI TTAA 8 cut(s) 531, 555, 732, 1187, 1964, 2133, 2252, 2726
MslI CAYNNNNRTG 3 cut(s) 389, 1910, 2022
Msp20I TGGCCA 1 cut(s) 1401
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 6 cut(s) 192, 312, 869, 1199, 2382, 2458
MspR9I CCNGG 4 cut(s) 281, 1381, 1776, 2382
MssI GTTTAAAC 1 cut(s) 556
MunI CAATTG 2 cut(s) 516, 2237
Mva1269I GAATGC 5 cut(s) 475, 688, 1090, 1390, 1693
MvaI CCWGG 3 cut(s) 281, 1381, 1776
MvnI CGCG 1 cut(s) 548
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 1 cut(s) 2382
NdeII GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
NlaIV GGNNCC 6 cut(s) 15, 271, 523, 1470, 2379, 2456
NmuCI GTSAC 4 cut(s) 886, 1177, 2546, 2786
NsbI TGCGCA 1 cut(s) 2771
NsiI ATGCAT 2 cut(s) 688, 2206
NspI RCATGY 5 cut(s) 1009, 2115, 2208, 2221, 2272
NspV TTCGAA 1 cut(s) 435
PaeI GCATGC 1 cut(s) 2272
PaeR7I CTCGAG 1 cut(s) 1038
PagI TCATGA 1 cut(s) 2650
PaqCI CACCTGC 1 cut(s) 1333
PceI AGGCCT 2 cut(s) 284, 593
PctI GAATGC 5 cut(s) 475, 688, 1090, 1390, 1693
PdmI GAANNNNTTC 2 cut(s) 458, 1088
PfeI GAWTC 7 cut(s) 419, 725, 940, 1543, 1580, 1767, 2639
PflFI GACNNNGTC 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 1379
PinAI ACCGGT 1 cut(s) 1198
PkrI GCNGC 9 cut(s) 208, 293, 317, 547, 1306, 1931, 2425, 2501, 2797
PleI GAGTC 3 cut(s) 1222, 2572, 2762
PluTI GGCGCC 1 cut(s) 273
PmeI GTTTAAAC 1 cut(s) 556
PpsI GAGTC 3 cut(s) 1222, 2572, 2762
Ppu21I YACGTR 1 cut(s) 1562
PsiI TTATAA 2 cut(s) 1997, 2328
Psp124BI GAGCTC 1 cut(s) 1379
Psp6I CCWGG 3 cut(s) 279, 1379, 1774
PspEI GGTNACC 1 cut(s) 1177
PspFI CCCAGC 3 cut(s) 908, 2123, 2221
PspGI CCWGG 3 cut(s) 279, 1379, 1774
PspN4I GGNNCC 6 cut(s) 15, 271, 523, 1470, 2379, 2456
PspPI GGNCC 1 cut(s) 2455
PspXI VCTCGAGB 1 cut(s) 1038
PstNI CAGNNNCTG 1 cut(s) 2642
PsuI RGATCY 2 cut(s) 1417, 1468
PsyI GACNNNGTC 1 cut(s) 365
RsaI GTAC 5 cut(s) 395, 488, 701, 1025, 1442
RsaNI GTAC 5 cut(s) 394, 487, 700, 1024, 1441
RseI CAYNNNNRTG 3 cut(s) 389, 1910, 2022
SacI GAGCTC 1 cut(s) 1379
SacII CCGCGG 1 cut(s) 549
SaqAI TTAA 8 cut(s) 531, 555, 732, 1187, 1964, 2133, 2252, 2726
SatI GCNGC 9 cut(s) 207, 292, 316, 546, 1305, 1930, 2424, 2500, 2796
Sau3AI GATC 5 cut(s) 585, 1135, 1417, 1468, 1738
Sau96I GGNCC 1 cut(s) 2455
SchI GAGTC 3 cut(s) 1223, 2573, 2763
ScrFI CCNGG 4 cut(s) 281, 1381, 1776, 2382
SduI GDGCHC 4 cut(s) 1379, 1513, 1801, 2382
SexAI ACCWGGT 1 cut(s) 1774
SfaNI GCATC 6 cut(s) 390, 1494, 1991, 2151, 2435, 2781
SfcI CTRYAG 1 cut(s) 2317
SfoI GGCGCC 1 cut(s) 271
Sfr274I CTCGAG 1 cut(s) 1038
Sfr303I CCGCGG 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 435
SgrBI CCGCGG 1 cut(s) 549
SinI GGWCC 1 cut(s) 2455
SlaI CTCGAG 1 cut(s) 1038
SmiMI CAYNNNNRTG 3 cut(s) 389, 1910, 2022
SmlI CTYRAG 2 cut(s) 490, 1038
SmoI CTYRAG 2 cut(s) 490, 1038
SnaBI TACGTA 1 cut(s) 1562
SphI GCATGC 1 cut(s) 2272
SseBI AGGCCT 2 cut(s) 284, 593
SspDI GGCGCC 1 cut(s) 269
SspI AATATT 2 cut(s) 2150, 2682
SspMI CTAG 4 cut(s) 1107, 1835, 2289, 2369
SstI GAGCTC 1 cut(s) 1379
StuI AGGCCT 2 cut(s) 284, 593
StyD4I CCNGG 4 cut(s) 279, 1379, 1774, 2380
TaaI ACNGT 5 cut(s) 1183, 1372, 1821, 1961, 2409
TaiI ACGT 2 cut(s) 884, 1564
TaqI TCGA 8 cut(s) 306, 435, 779, 1039, 1634, 1737, 1747, 2411
TatI WGTACW 1 cut(s) 393
TauI GCSGC 5 cut(s) 209, 294, 318, 548, 2502
TfiI GAWTC 7 cut(s) 419, 725, 940, 1543, 1580, 1767, 2639
Tru1I TTAA 8 cut(s) 531, 555, 732, 1187, 1964, 2133, 2252, 2726
Tru9I TTAA 8 cut(s) 531, 555, 732, 1187, 1964, 2133, 2252, 2726
TscAI CASTG 5 cut(s) 470, 1377, 1683, 1806, 2754
TseFI GTSAC 4 cut(s) 886, 1177, 2546, 2786
TseI GCWGC 4 cut(s) 1304, 1929, 2423, 2795
Tsp45I GTSAC 4 cut(s) 886, 1177, 2546, 2786
TspGWI ACGGA 2 cut(s) 1046, 2840
TspRI CASTG 5 cut(s) 470, 1377, 1683, 1806, 2754
Tth111I GACNNNGTC 1 cut(s) 365
VneI GTGCAC 1 cut(s) 1797
VpaK11BI GGWCC 1 cut(s) 2455
XagI CCTNNNNNAGG 1 cut(s) 278
XapI RAATTY 7 cut(s) 707, 775, 1520, 1671, 2013, 2100, 2292
XbaI TCTAGA 1 cut(s) 1106
XceI RCATGY 5 cut(s) 1009, 2115, 2208, 2221, 2272
XcmI CCANNNNNNNNNTGG 1 cut(s) 1409
XhoI CTCGAG 1 cut(s) 1038
XmiI GTMKAC 1 cut(s) 2214
XmnI GAANNNNTTC 2 cut(s) 458, 1088
XspI CTAG 4 cut(s) 1107, 1835, 2289, 2369
Zsp2I ATGCAT 2 cut(s) 688, 2206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.