MD15G1377500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000497
Physical Location & Seq
Reverse (-)
46229449 .. 46232386
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1377500.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGATCTTCCGCGGGAAATTGACGACTATATCAAAGAATCGATCGACCACTCCTTGGGCCTCCCTGTGTCGACGCACACCCTCGAATTGAAGCTTCGATGCTCCGAAGAAGTGAAACGGCGGCTTCAGAATCAGTATTCACTACTCCTCTCGAAACTGAAGGAGAAAGAGCAAGTCCTGGAGCGCTATAAGGCCGAAGCGAGTATGAATGCACAAGCTTTGAGGAAGTTCGTTGAGGAGAATCAGAGATTGGCGTCGGAATGTGCGCATCTGGTGACTCAGTGCAACAAGTGGGAGAGAGAATGCTCGCTCTACGATCACGACAGAGAGGCTTTAATGGATTTTGGGAACGAGGCTGATCAGCGTGCCAAGGAGGCGGAGATTCGTGTTCAGGAGTTGGAGGACGAAGTCAGAGAGTTGAGTGATGAATTGGGGTTCTACAAGCACAAATTGGAGATGCATTCGGTTGAATCATCTGCTGAAGACACAACCATTGAAGAGAAACTACTGGATTCTGTTTTGGCAACATTGATCAATACAGATGAAGCTGTGTCTGCACGTGCTTTTCTGGAGGCCAACAAAGGAAATGAGTCATGTGGAAGGTTGCTAAAAATGTGGAACTGCTTGAAGCCGTCATCTCAAAAAGTTCTATCACTAGTTGCTGAGGTAAAGACGCTTGAGAAGGATAAGGAACATCTAAGAATCAATCTTCGGACGGCTGAAGAAGAGGTCAAATTGCTTTTTGAAGAAAACAAAGTCCTAGATGTGGAGAACAAAAGATTACTGAGGCTATACCAGAAGGAAAGAAACCATTCTGCGTCTGGTGGAAAACATATTGACAGTGCATCAGCGAAGTCAAATAAGCGCAAATCAAGCTCTAACATAAGCAGCCCGATTCAAGGGAAGATTGATTTCAGTGAGCAAGAAGCAGCAAGACAGCCCCTATCACCCTTGCGCTGTAACTCCCCTAACTCTAGAACCTATAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

37.95

Weight (kDa)

5.96

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14680 AT4G09060 AT4G09060
fragaria_vesca FvH4_5g36300 FvH4_5g36300
malus_domestica MD08G1189700.v1.1 MD08G1190000.v1.1 MD08G1190200.v1.1 MD15G1377500.v1.1
prunus_persica Prupe.1G524200_v2.0.a1
pyrus_communis pycom08g16320 pycom15g33820
rosa_chinensis RchiOBHm_Chr3g0481851 RchiOBHm_Chr3g0481861 RchiOBHm_Chr5g0040961 RchiOBHm_Chr5g0044491 RchiOBHm_Chr5g0044811 RchiOBHm_Chr5g0045611 RchiOBHm_Chr5g0045621 RchiOBHm_Chr5g0049341 RchiOBHm_Chr5g0049351 RchiOBHm_Chr7g0238301 RchiOBHm_Chr7g0238321
rosa_laevigata RLG00000000918 RLG00000000921 RLG00000034299 RLG00000034300
rosa_multiflora Rmu_sc0001047.1_g000051 Rmu_sc0001371.1_g000020 Rmu_sc0001847.1_g000002 Rmu_sc0002988.1_g000004 Rmu_sc0003789.1_g000001 Rmu_sc0004567.1_g000016 Rmu_sc0004800.1_g000003 Rmu_sc0005102.1_g000001 Rmu_sc0006651.1_g000010 Rmu_sc0006651.1_g000011 Rmu_ssc0000398.1_g000017
rosa_roxburghii Rroxscaffold_153G00436700 Rroxscaffold_153G00436710 Rroxscaffold_1G00034810 Rroxscaffold_1G00036220 Rroxscaffold_1G00036230 Rroxscaffold_1G00074790 Rroxscaffold_2G00104790 Rroxscaffold_2G00104800 Rroxscaffold_3G00223120 Rroxscaffold_5G00337240 Rroxscaffold_6G00399830 Rroxscaffold_6G00400170
rosa_rugosa Rorug02G0148000 Rorug04G0003000 Rorug05G0218300 Rorug05G0218400 Rorug05G0219900 Rorug07G0311100 Rorug07G0311900
rosa_samantha Rh2BG555200 Rh3DG293700 Rh4BG116500 Rh5BG279700 Rh5BG308000 Rh5BG308100 Rh5BG313100 Rh5BG313200 Rh5BG335300 Rh5BG335400 Rh5CG335000 Rh5CG335100 Rh5DG287700 Rh5DG318100 Rh5DG318200 Rh5DG323600 Rh5DG323700 Rh5DG347600 Rh5DG347700 Rh6DG317800 Rh7BG437500 Rh7CG484600 Rh7DG453000
rosa_wichuraiana Rw0G004340 Rw5G025810 Rw5G027210 Rw5G027900 Rw5G028300 Rw5G030640 Rw7G038670 Rw7G038780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 55
AccI GTMKAC 1 cut(s) 71
AccII CGCG 1 cut(s) 13
AciI CCGC 4 cut(s) 11, 13, 121, 377
AclWI GGATC 1 cut(s) 12
AcuI CTGAAG 4 cut(s) 110, 179, 501, 741
AcvI CACGTG 1 cut(s) 560
AcyI GRCGYC 1 cut(s) 254
AfeI AGCGCT 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 55, 766, 899
AgsI TTSAA 6 cut(s) 91, 470, 497, 628, 746, 899
AhlI ACTAGT 1 cut(s) 655
AjnI CCWGG 1 cut(s) 177
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 4 cut(s) 94, 218, 548, 876
AluI AGCT 4 cut(s) 94, 218, 548, 876
AlwI GGATC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 185
AoxI GGCC 3 cut(s) 58, 192, 573
ApeKI GCWGC 2 cut(s) 888, 929
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 4 cut(s) 186, 268, 867, 957
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 286, 939
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 489
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 2 cut(s) 900, 941
BceAI ACGGC 3 cut(s) 134, 616, 732
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 2 cut(s) 358, 531
BcuI ACTAGT 1 cut(s) 655
BfaI CTAG 3 cut(s) 656, 761, 975
BfoI RGCGCY 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 3 cut(s) 122, 889, 930
BlsI GCNGC 3 cut(s) 123, 890, 931
Bme1390I CCNGG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 4 cut(s) 89, 277, 447, 854
BpiI GAAGAC 1 cut(s) 489
BpmI CTGGAG 2 cut(s) 200, 590
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 698
Bsa29I ATCGAT 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 560
BsaHI GRCGYC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 11, 54, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 766, 899
Bse1I ACTGG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 3 cut(s) 11, 54, 369
BseLI CCNNNNNNNGG 3 cut(s) 55, 766, 899
BseMII CTCAG 3 cut(s) 293, 654, 776
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 2 cut(s) 137, 251
BseXI GCAGC 2 cut(s) 900, 941
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 3 cut(s) 60, 194, 575
BshVI ATCGAT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 55, 766, 899
BsmI GAATGC 3 cut(s) 214, 308, 460
BsnI GGCC 3 cut(s) 60, 194, 575
Bsp143I GATC 5 cut(s) 4, 42, 316, 358, 531
BspACI CCGC 4 cut(s) 11, 13, 121, 377
BspANI GGCC 3 cut(s) 60, 194, 575
BspCNI CTCAG 3 cut(s) 292, 655, 777
BspDI ATCGAT 1 cut(s) 41
BspFNI CGCG 1 cut(s) 13
BspPI GGATC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 11, 54, 369
BssMI GATC 5 cut(s) 4, 42, 316, 358, 531
BssNI GRCGYC 1 cut(s) 254
BssT1I CCWWGG 2 cut(s) 54, 369
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 842
Bst6I CTCTTC 2 cut(s) 492, 720
BstACI GRCGYC 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 2 cut(s) 308, 366
BstDEI CTNAG 4 cut(s) 279, 663, 698, 785
BstDSI CCRYGG 1 cut(s) 11
BstFNI CGCG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 4 cut(s) 186, 268, 867, 957
BstKTI GATC 5 cut(s) 7, 45, 319, 361, 534
BstMBI GATC 5 cut(s) 4, 42, 316, 358, 531
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 374, 554, 873
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 177
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 900, 941
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 41
BsuRI GGCC 3 cut(s) 60, 194, 575
BsuTUI ATCGAT 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 11
BtsIMutI CAGTG 3 cut(s) 287, 847, 922
Cac8I GCNNGC 2 cut(s) 308, 366
CfoI GCGC 4 cut(s) 186, 268, 867, 957
Cfr13I GGNCC 1 cut(s) 58
Cfr42I CCGCGG 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 41
CseI GACGC 4 cut(s) 82, 243, 682, 807
CviAII CATG 1 cut(s) 594
DdeI CTNAG 4 cut(s) 279, 663, 698, 785
DpnI GATC 5 cut(s) 6, 44, 318, 360, 533
DpnII GATC 5 cut(s) 4, 42, 316, 358, 531
Eam1104I CTCTTC 2 cut(s) 492, 720
EarI CTCTTC 2 cut(s) 492, 720
EciI GGCGGA 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 54, 369
Eco47III AGCGCT 1 cut(s) 185
Eco57I CTGAAG 4 cut(s) 110, 179, 501, 741
Eco72I CACGTG 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 54, 369
EcoT22I ATGCAT 1 cut(s) 462
ErhI CCWWGG 2 cut(s) 54, 369
FaeI CATG 1 cut(s) 597
FaiI YATR 8 cut(s) 30, 189, 206, 595, 793, 834, 884, 984
FatI CATG 1 cut(s) 593
FauI CCCGC 1 cut(s) 6
FbaI TGATCA 2 cut(s) 358, 531
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 3 cut(s) 122, 889, 930
Fsp4HI GCNGC 3 cut(s) 122, 889, 930
FspAI RTGCGCAY 1 cut(s) 267
FspBI CTAG 3 cut(s) 656, 761, 975
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 4 cut(s) 185, 267, 866, 956
GluI GCNGC 3 cut(s) 122, 889, 930
GsuI CTGGAG 2 cut(s) 200, 590
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 3 cut(s) 60, 194, 575
HgaI GACGC 4 cut(s) 82, 243, 682, 807
HhaI GCGC 4 cut(s) 186, 268, 867, 957
Hin1I GRCGYC 1 cut(s) 254
Hin1II CATG 1 cut(s) 597
Hin6I GCGC 4 cut(s) 184, 266, 865, 955
HinP1I GCGC 4 cut(s) 184, 266, 865, 955
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HindIII AAGCTT 2 cut(s) 92, 216
HphI GGTGA 2 cut(s) 286, 939
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 6 cut(s) 106, 129, 246, 259, 413, 714
Hpy188III TCNNGA 5 cut(s) 151, 320, 392, 569, 975
Hpy8I GTNNAC 1 cut(s) 72
Hpy99I CGWCG 2 cut(s) 76, 259
HpyAV CCTTC 4 cut(s) 154, 594, 676, 793
HpyCH4III ACNGT 1 cut(s) 842
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 5 cut(s) 212, 285, 460, 557, 845
HpyF10VI GCNNNNNNNGC 3 cut(s) 374, 554, 873
HpyF3I CTNAG 4 cut(s) 279, 663, 698, 785
HpySE526I ACGT 1 cut(s) 559
Hsp92I GRCGYC 1 cut(s) 254
Hsp92II CATG 1 cut(s) 597
HspAI GCGC 4 cut(s) 184, 266, 865, 955
Ksp22I TGATCA 2 cut(s) 358, 531
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 5 cut(s) 4, 42, 316, 358, 531
LmnI GCTCC 2 cut(s) 107, 181
LpnPI CCDG 9 cut(s) 78, 164, 191, 257, 377, 494, 554, 807, 809
Lsp1109I GCAGC 2 cut(s) 900, 941
LweI GCATC 4 cut(s) 89, 277, 447, 854
MaeI CTAG 3 cut(s) 656, 761, 975
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 2 cut(s) 274, 959
MalI GATC 5 cut(s) 6, 44, 318, 360, 533
MboI GATC 5 cut(s) 4, 42, 316, 358, 531
MboII GAAGA 8 cut(s) 119, 494, 509, 701, 734, 737, 758, 916
MflI RGATCY 1 cut(s) 4
MluCI AATT 5 cut(s) 18, 86, 428, 449, 734
MlyI GAGTC 2 cut(s) 271, 599
MmeI TCCRAC 2 cut(s) 237, 378
Mph1103I ATGCAT 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 179
Mva1269I GAATGC 3 cut(s) 214, 308, 460
MvaI CCWGG 1 cut(s) 179
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 374, 554, 873
NdeII GATC 5 cut(s) 4, 42, 316, 358, 531
NlaIII CATG 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 274
NsbI TGCGCA 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 462
PctI GAATGC 3 cut(s) 214, 308, 460
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 8 cut(s) 38, 130, 241, 382, 470, 512, 702, 895
PflFI GACNNNGTC 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 55
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 3 cut(s) 123, 890, 931
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 2 cut(s) 271, 598
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 2 cut(s) 271, 598
Ppu21I YACGTR 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 177
PspCI CACGTG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 177
PspPI GGNCC 1 cut(s) 58
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 407
PvuI CGATCG 1 cut(s) 45
SacII CCGCGG 1 cut(s) 14
SalI GTCGAC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 3 cut(s) 122, 889, 930
Sau3AI GATC 5 cut(s) 4, 42, 316, 358, 531
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 2 cut(s) 271, 599
ScrFI CCNGG 1 cut(s) 179
SetI ASST 9 cut(s) 96, 220, 550, 562, 605, 669, 732, 878, 983
SfaNI GCATC 4 cut(s) 89, 277, 447, 854
Sfr303I CCGCGG 1 cut(s) 14
SgrBI CCGCGG 1 cut(s) 14
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
SpeI ACTAGT 1 cut(s) 655
Sse9I AATT 5 cut(s) 18, 86, 428, 449, 734
SsiI CCGC 4 cut(s) 11, 13, 121, 377
SspMI CTAG 3 cut(s) 656, 761, 975
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 2 cut(s) 54, 369
TaaI ACNGT 1 cut(s) 842
TaiI ACGT 1 cut(s) 562
TaqI TCGA 6 cut(s) 41, 45, 71, 84, 97, 152
TasI AATT 5 cut(s) 18, 86, 428, 449, 734
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 8 cut(s) 38, 130, 241, 382, 470, 512, 702, 895
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TscAI CASTG 3 cut(s) 287, 847, 922
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 888, 929
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 221, 441, 558
TspRI CASTG 3 cut(s) 287, 847, 922
Tth111I GACNNNGTC 1 cut(s) 407
Van91I CCANNNNNTGG 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 3 cut(s) 656, 761, 975
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.