Rw5G028300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000497
Physical Location & Seq
Reverse (-)
42814337 .. 42816971
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028300.1

Sequence Viewer

Length: 963 bp
ATGGATCCTCTTCCTCAAGAAGTCGACGATTACATCAGAGAGTCGATTGACCACACCCTCGGCCTCCCCGTGTCCGCACAGACTCTCGAATTGAAGAAGCTCCGGTGCTCGGAAGAAGGATCAAGCTCTGGACCGATTTGGTTGGGTTGCTTATGTGCTTTGAGGAAGTTTGTGGAGGAGAATCAGAGACTAGCGGCAGAGTGTGCGCATCTGGTGAGTCGGTGTAACAAGTTGGAGAAGGAGTGCTCGCTCTATGATCATGACCGAGAGGCTTTGATGGATTTCGGAACCAGAGGGCCAAGGAGGCTGATCTCTTTTACTATGATTTTGCGAAGGCTGATGAATATTTCTATGTTGAAGGTTGATTCATCTGCTGAAGACTCACCTGTTGAAGAGAAACTAGTGGAGTCTGTTTTAGCAACATCGACCAAAGATGAGACTGGGTCATGTCAAAGGTTGCTAAATATGTGGAATGGCTTGAGGCCGCCATCTCAAAAAGTTTTGTCGCTAGTTGCTGAGTTGAAAACATTTCAGAAGAATAATGAAACACTTGAGAAGGATAAAGAACATCTAAGGATCAATCTTCTTTCTGCTGAAGAGGAGGTCAAATTGCTTTTCAAAGAAAACAAAGTGTTAGATGAAGCTAACAAAAGATTACTAAAGCAGTACCGCAAGGAAAGAAACAATTCTGGTTCTGATGGAAAGCATACTGATGTATCAACGAAGTCAAACAAGCGAAAATCTAGCTCTAAGGTGATGAGCAGCGGCCCGATTCAGGGGAAGATCGATTTCAGTGACCAAGAATCAGCAAGACAACCTCTCTCACCCTTGCGTTGTAACTCCCCTAACTCAAGACTGCATAAAAATCAAGGAAGCTTCCGAAATAGTCTCACCTCTCATTTGTACCTTTTTATGTACCCATCATATGGGTTGGTTGGCATAAGATTGCAGATTTTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

320

Amino Acids

36.39

Weight (kDa)

8.9

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14680 AT4G09060 AT4G09060
fragaria_vesca FvH4_5g36300 FvH4_5g36300
malus_domestica MD08G1189700.v1.1 MD08G1190000.v1.1 MD08G1190200.v1.1 MD15G1377500.v1.1
prunus_persica Prupe.1G524200_v2.0.a1
pyrus_communis pycom08g16320 pycom15g33820
rosa_chinensis RchiOBHm_Chr3g0481851 RchiOBHm_Chr3g0481861 RchiOBHm_Chr5g0040961 RchiOBHm_Chr5g0044491 RchiOBHm_Chr5g0044811 RchiOBHm_Chr5g0045611 RchiOBHm_Chr5g0045621 RchiOBHm_Chr5g0049341 RchiOBHm_Chr5g0049351 RchiOBHm_Chr7g0238301 RchiOBHm_Chr7g0238321
rosa_laevigata RLG00000000918 RLG00000000921 RLG00000034299 RLG00000034300
rosa_multiflora Rmu_sc0001047.1_g000051 Rmu_sc0001371.1_g000020 Rmu_sc0001847.1_g000002 Rmu_sc0002988.1_g000004 Rmu_sc0003789.1_g000001 Rmu_sc0004567.1_g000016 Rmu_sc0004800.1_g000003 Rmu_sc0005102.1_g000001 Rmu_sc0006651.1_g000010 Rmu_sc0006651.1_g000011 Rmu_ssc0000398.1_g000017
rosa_roxburghii Rroxscaffold_153G00436700 Rroxscaffold_153G00436710 Rroxscaffold_1G00034810 Rroxscaffold_1G00036220 Rroxscaffold_1G00036230 Rroxscaffold_1G00074790 Rroxscaffold_2G00104790 Rroxscaffold_2G00104800 Rroxscaffold_3G00223120 Rroxscaffold_5G00337240 Rroxscaffold_6G00399830 Rroxscaffold_6G00400170
rosa_rugosa Rorug02G0148000 Rorug04G0003000 Rorug05G0218300 Rorug05G0218400 Rorug05G0219900 Rorug07G0311100 Rorug07G0311900
rosa_samantha Rh2BG555200 Rh3DG293700 Rh4BG116500 Rh5BG279700 Rh5BG308000 Rh5BG308100 Rh5BG313100 Rh5BG313200 Rh5BG335300 Rh5BG335400 Rh5CG335000 Rh5CG335100 Rh5DG287700 Rh5DG318100 Rh5DG318200 Rh5DG323600 Rh5DG323700 Rh5DG347600 Rh5DG347700 Rh6DG317800 Rh7BG437500 Rh7CG484600 Rh7DG453000
rosa_wichuraiana Rw0G004340 Rw5G025810 Rw5G027210 Rw5G027900 Rw5G028300 Rw5G030640 Rw7G038670 Rw7G038780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 926
AccI GTMKAC 1 cut(s) 24
AciI CCGC 5 cut(s) 75, 194, 485, 670, 765
AclWI GGATC 3 cut(s) 12, 127, 584
AcuI CTGAAG 2 cut(s) 396, 615
AfaI GTAC 3 cut(s) 668, 905, 917
AfiI CCNNNNNNNGG 4 cut(s) 109, 775, 776, 926
AgsI TTSAA 6 cut(s) 94, 358, 392, 523, 619, 958
AhlI ACTAGT 1 cut(s) 400
AluBI AGCT 5 cut(s) 100, 126, 644, 747, 876
AluI AGCT 5 cut(s) 100, 126, 644, 747, 876
Alw21I GWGCWC 2 cut(s) 110, 248
Alw26I GTCTC 3 cut(s) 181, 431, 893
AlwI GGATC 3 cut(s) 12, 127, 584
AoxI GGCC 4 cut(s) 61, 296, 482, 766
ApeKI GCWGC 1 cut(s) 762
Asp700I GAANNNNTTC 1 cut(s) 685
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 3 cut(s) 131, 296, 767
AsuHPI GGTGA 5 cut(s) 226, 375, 766, 816, 883
AvaII GGWCC 1 cut(s) 131
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 2 cut(s) 110, 248
BbvI GCAGC 1 cut(s) 774
BccI CCATC 4 cut(s) 271, 496, 692, 928
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 181, 431, 893
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 4 cut(s) 191, 401, 509, 744
BglI GCCNNNNNGGC 1 cut(s) 304
BisI GCNGC 4 cut(s) 195, 485, 763, 766
BlsI GCNGC 4 cut(s) 196, 486, 764, 767
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 3 cut(s) 131, 296, 767
BmiI GGNNCC 2 cut(s) 6, 289
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 217
BmuI ACTGGG 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 384
BpuEI CTTGAG 3 cut(s) 499, 572, 835
Bsa29I ATCGAT 1 cut(s) 786
BsaJI CCNNGG 2 cut(s) 58, 299
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 775, 776, 926
Bse1I ACTGG 1 cut(s) 445
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 58, 299
BseLI CCNNNNNNNGG 4 cut(s) 109, 775, 776, 926
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 2 cut(s) 191, 614
BseXI GCAGC 1 cut(s) 774
BshFI GGCC 4 cut(s) 63, 298, 484, 768
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 2 cut(s) 110, 248
BsiSI CCGG 1 cut(s) 103
BslI CCNNNNNNNGG 4 cut(s) 109, 775, 776, 926
BsmAI GTCTC 3 cut(s) 181, 431, 893
BsnI GGCC 4 cut(s) 63, 298, 484, 768
Bsp1286I GDGCHC 2 cut(s) 110, 248
Bsp143I GATC 6 cut(s) 4, 119, 256, 309, 576, 783
BspACI CCGC 5 cut(s) 75, 194, 485, 670, 765
BspANI GGCC 4 cut(s) 63, 298, 484, 768
BspCNI CTCAG 1 cut(s) 508
BspDI ATCGAT 1 cut(s) 786
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 2 cut(s) 6, 289
BspPI GGATC 3 cut(s) 12, 127, 584
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 58, 299
BssMI GATC 6 cut(s) 4, 119, 256, 309, 576, 783
BssT1I CCWWGG 1 cut(s) 299
Bst6I CTCTTC 3 cut(s) 15, 387, 591
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 3 cut(s) 516, 572, 750
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 6 cut(s) 7, 122, 259, 312, 579, 786
BstMAI GTCTC 3 cut(s) 181, 431, 893
BstMBI GATC 6 cut(s) 4, 119, 256, 309, 576, 783
BstMWI GCNNNNNNNGC 2 cut(s) 203, 304
BstV1I GCAGC 1 cut(s) 774
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 786
BsuRI GGCC 4 cut(s) 63, 298, 484, 768
BsuTUI ATCGAT 1 cut(s) 786
BtsIMutI CAGTG 1 cut(s) 799
Cac8I GCNNGC 1 cut(s) 248
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 3 cut(s) 131, 296, 767
ClaI ATCGAT 1 cut(s) 786
Csp6I GTAC 3 cut(s) 667, 904, 916
CviAII CATG 2 cut(s) 260, 447
CviQI GTAC 3 cut(s) 667, 904, 916
DdeI CTNAG 3 cut(s) 516, 572, 750
DpnI GATC 6 cut(s) 6, 121, 258, 311, 578, 785
DpnII GATC 6 cut(s) 4, 119, 256, 309, 576, 783
Eam1104I CTCTTC 3 cut(s) 15, 387, 591
EarI CTCTTC 3 cut(s) 15, 387, 591
Eco130I CCWWGG 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 2 cut(s) 396, 615
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 2 cut(s) 263, 450
FatI CATG 2 cut(s) 259, 446
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 4 cut(s) 195, 485, 763, 766
Fsp4HI GCNGC 4 cut(s) 195, 485, 763, 766
FspAI RTGCGCAY 1 cut(s) 207
FspBI CTAG 4 cut(s) 191, 401, 509, 744
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 1 cut(s) 207
GluI GCNGC 4 cut(s) 195, 485, 763, 766
HaeIII GGCC 4 cut(s) 63, 298, 484, 768
HapII CCGG 1 cut(s) 103
HhaI GCGC 1 cut(s) 208
Hin1II CATG 2 cut(s) 263, 450
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 9 cut(s) 41, 82, 181, 217, 365, 380, 407, 772, 803
HpaII CCGG 1 cut(s) 103
HphI GGTGA 5 cut(s) 226, 375, 766, 816, 883
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 7 cut(s) 38, 112, 186, 287, 534, 697, 881
Hpy188III TCNNGA 5 cut(s) 17, 86, 129, 260, 852
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 5 cut(s) 110, 232, 327, 352, 550
HpyCH4V TGCA 2 cut(s) 859, 949
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 304
HpyF3I CTNAG 3 cut(s) 516, 572, 750
Hsp92II CATG 2 cut(s) 263, 450
HspAI GCGC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 6 cut(s) 4, 119, 256, 309, 576, 783
LmnI GCTCC 1 cut(s) 105
LpnPI CCDG 8 cut(s) 114, 116, 197, 304, 399, 426, 675, 761
Lsp1109I GCAGC 1 cut(s) 774
LweI GCATC 1 cut(s) 217
MaeI CTAG 4 cut(s) 191, 401, 509, 744
MaeIII GTNAC 3 cut(s) 224, 794, 836
MalI GATC 6 cut(s) 6, 121, 258, 311, 578, 785
MboI GATC 6 cut(s) 4, 119, 256, 309, 576, 783
MboII GAAGA 8 cut(s) 106, 125, 389, 404, 547, 575, 608, 793
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 110, 248
MluCI AATT 3 cut(s) 89, 608, 685
MlyI GAGTC 5 cut(s) 50, 76, 226, 374, 416
MmeI TCCRAC 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 685
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 1 cut(s) 765
MspI CCGG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 203, 304
NdeI CATATG 1 cut(s) 925
NdeII GATC 6 cut(s) 4, 119, 256, 309, 576, 783
NlaIII CATG 2 cut(s) 263, 450
NlaIV GGNNCC 2 cut(s) 6, 289
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 794
NsbI TGCGCA 1 cut(s) 207
PagI TCATGA 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 685
PfeI GAWTC 4 cut(s) 181, 365, 772, 803
PflFI GACNNNGTC 1 cut(s) 442
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 4 cut(s) 196, 486, 764, 767
PleI GAGTC 5 cut(s) 49, 76, 225, 374, 415
PpsI GAGTC 5 cut(s) 49, 76, 225, 374, 415
PspN4I GGNNCC 2 cut(s) 6, 289
PspPI GGNCC 3 cut(s) 131, 296, 767
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 442
RsaI GTAC 3 cut(s) 668, 905, 917
RsaNI GTAC 3 cut(s) 667, 904, 916
RseI CAYNNNNRTG 1 cut(s) 711
SalI GTCGAC 1 cut(s) 23
SatI GCNGC 4 cut(s) 195, 485, 763, 766
Sau3AI GATC 6 cut(s) 4, 119, 256, 309, 576, 783
Sau96I GGNCC 3 cut(s) 131, 296, 767
SchI GAGTC 5 cut(s) 50, 76, 226, 374, 416
SduI GDGCHC 2 cut(s) 110, 248
SfaNI GCATC 1 cut(s) 217
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 4 cut(s) 15, 478, 551, 850
SmoI CTYRAG 4 cut(s) 15, 478, 551, 850
SpeI ACTAGT 1 cut(s) 400
Sse9I AATT 3 cut(s) 89, 608, 685
SsiI CCGC 5 cut(s) 75, 194, 485, 670, 765
SspI AATATT 1 cut(s) 346
SspMI CTAG 4 cut(s) 191, 401, 509, 744
StyI CCWWGG 1 cut(s) 299
TaqI TCGA 5 cut(s) 24, 44, 87, 425, 786
TaqII GACCGA 2 cut(s) 148, 279
TasI AATT 3 cut(s) 89, 608, 685
TauI GCSGC 3 cut(s) 197, 487, 768
TfiI GAWTC 4 cut(s) 181, 365, 772, 803
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 1 cut(s) 762
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 4 cut(s) 356, 357, 558, 654
TspRI CASTG 1 cut(s) 799
Tth111I GACNNNGTC 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 24
XmnI GAANNNNTTC 1 cut(s) 685
XspI CTAG 4 cut(s) 191, 401, 509, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.