pycom08g16320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000497
Physical Location & Seq
Reverse (-)
16217777 .. 16220512
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16320.6

Sequence Viewer

Length: 993 bp
ATGAATCTTCCGCAAGAAATCGACGACTATATCAAAGAATCGATCGACCACTCCTTGGGCCTCCCTGTGTCGACGCACACCCTCGAATTGAAGTTTCGATGCTCCGAAGAAGCGAAACGGCGGCTTCAGAATCAGTACTCGCTTCTTCTCTCGAAATTGAAGAAGAAAGAGCAAGTCCTGGAGCGCGCTAAGGCCGAAGCGAGTATGAATGCACAAGCTTTGAGGAAGTTCGTTGAGGAGAATCAGAAATTGGCATTGGAGTGTGCGCACCTGGTGACTCAATGCAACAAGTGGGAGAGGGAGTGCTCGCTCTACGATCATGACCGAGAGGCTTTGATGGATTTTGGGAACGAGGCTGATCAGCGTGCCAAGGAGGCCGAGATTCGTGTTCAAGAGTTGGAGGACGAAGTCAGAGAGTTGAGGGATGAATTGGGGTTATACAAGTACAAAATGGAGATGCATTCAGTTGATTCATCTGCTGAAGACACAGCAATTGAAGACAAACTACTGGATTCTGTTTTGGCAACATTGATCAGTAAAGATGAAGCTGTGTCTGCACGTGCGTTTCTGGAGGCCAACAAAGGCAATGAGTCATGTGCAAGATTACTAAAAATGTGGAACTGCTTGAAGCCGTCGTCTCAAAAAGTTCTATCACTAGTTGCTGAGGTAAAGACACTTGAGAAGGATAAGGAACATCTTAGGATCAATCTTCGTACGGCTGAAGAGGAGGTCAAATTGCTTTTTGAAGAAAACAAAGTCCTAGATGTGGAGAACAAAAGATTACTGAGGCTGTACCAGAAGGAAAGAAACCATTCTGCCTCTGGTGGAAAACATACTGACAGTGCATCAGCGAAGTCAAATAAGCGCAAATCAAGCTCTAAGACAAGCAGCCCGATTCAAGGGAAGATTGATTTCAGTGAGCAGGAAGCAGTAAGACAGCCCCTATCACCCTTGCTCTGTAACTCCCCTAACTCTAGAACCTCTAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

37.79

Weight (kDa)

6.5

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14680 AT4G09060 AT4G09060
fragaria_vesca FvH4_5g36300 FvH4_5g36300
malus_domestica MD08G1189700.v1.1 MD08G1190000.v1.1 MD08G1190200.v1.1 MD15G1377500.v1.1
prunus_persica Prupe.1G524200_v2.0.a1
pyrus_communis pycom08g16320 pycom15g33820
rosa_chinensis RchiOBHm_Chr3g0481851 RchiOBHm_Chr3g0481861 RchiOBHm_Chr5g0040961 RchiOBHm_Chr5g0044491 RchiOBHm_Chr5g0044811 RchiOBHm_Chr5g0045611 RchiOBHm_Chr5g0045621 RchiOBHm_Chr5g0049341 RchiOBHm_Chr5g0049351 RchiOBHm_Chr7g0238301 RchiOBHm_Chr7g0238321
rosa_laevigata RLG00000000918 RLG00000000921 RLG00000034299 RLG00000034300
rosa_multiflora Rmu_sc0001047.1_g000051 Rmu_sc0001371.1_g000020 Rmu_sc0001847.1_g000002 Rmu_sc0002988.1_g000004 Rmu_sc0003789.1_g000001 Rmu_sc0004567.1_g000016 Rmu_sc0004800.1_g000003 Rmu_sc0005102.1_g000001 Rmu_sc0006651.1_g000010 Rmu_sc0006651.1_g000011 Rmu_ssc0000398.1_g000017
rosa_roxburghii Rroxscaffold_153G00436700 Rroxscaffold_153G00436710 Rroxscaffold_1G00034810 Rroxscaffold_1G00036220 Rroxscaffold_1G00036230 Rroxscaffold_1G00074790 Rroxscaffold_2G00104790 Rroxscaffold_2G00104800 Rroxscaffold_3G00223120 Rroxscaffold_5G00337240 Rroxscaffold_6G00399830 Rroxscaffold_6G00400170
rosa_rugosa Rorug02G0148000 Rorug04G0003000 Rorug05G0218300 Rorug05G0218400 Rorug05G0219900 Rorug07G0311100 Rorug07G0311900
rosa_samantha Rh2BG555200 Rh3DG293700 Rh4BG116500 Rh5BG279700 Rh5BG308000 Rh5BG308100 Rh5BG313100 Rh5BG313200 Rh5BG335300 Rh5BG335400 Rh5CG335000 Rh5CG335100 Rh5DG287700 Rh5DG318100 Rh5DG318200 Rh5DG323600 Rh5DG323700 Rh5DG347600 Rh5DG347700 Rh6DG317800 Rh7BG437500 Rh7CG484600 Rh7DG453000
rosa_wichuraiana Rw0G004340 Rw5G025810 Rw5G027210 Rw5G027900 Rw5G028300 Rw5G030640 Rw7G038670 Rw7G038780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 55
AccI GTMKAC 1 cut(s) 71
AccII CGCG 1 cut(s) 186
AciI CCGC 2 cut(s) 11, 121
AclWI GGATC 1 cut(s) 710
AcuI CTGAAG 3 cut(s) 110, 501, 741
AcvI CACGTG 1 cut(s) 560
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 4 cut(s) 137, 446, 715, 794
AfiI CCNNNNNNNGG 4 cut(s) 55, 766, 899, 987
AgsI TTSAA 7 cut(s) 91, 160, 392, 497, 628, 746, 899
AhlI ACTAGT 1 cut(s) 655
AjnI CCWGG 2 cut(s) 177, 270
AjuI GAANNNNNNNTTGG 4 cut(s) 233, 239, 265, 271
AluBI AGCT 3 cut(s) 218, 548, 876
AluI AGCT 3 cut(s) 218, 548, 876
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 1 cut(s) 642
AlwI GGATC 1 cut(s) 710
AoxI GGCC 4 cut(s) 58, 192, 375, 573
ApeKI GCWGC 1 cut(s) 888
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 4 cut(s) 186, 188, 268, 867
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 286, 939
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbrPI CACGTG 1 cut(s) 560
BbsI GAAGAC 2 cut(s) 489, 504
Bbv12I GWGCWC 1 cut(s) 308
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 1 cut(s) 900
BccI CCATC 1 cut(s) 331
BceAI ACGGC 3 cut(s) 134, 616, 732
BciT130I CCWGG 2 cut(s) 179, 272
BclI TGATCA 2 cut(s) 358, 531
BcoDI GTCTC 1 cut(s) 642
BcuI ACTAGT 1 cut(s) 655
BfaI CTAG 3 cut(s) 656, 761, 975
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 2 cut(s) 122, 889
BlsI GCNGC 2 cut(s) 123, 890
BmcAI AGTACT 1 cut(s) 137
Bme1390I CCNGG 2 cut(s) 179, 272
BmgT120I GGNCC 1 cut(s) 58
BmrFI CCNGG 2 cut(s) 179, 272
BmsI GCATC 3 cut(s) 89, 447, 854
BpiI GAAGAC 2 cut(s) 489, 504
BpmI CTGGAG 2 cut(s) 200, 590
Bpu10I CCTNAGC 2 cut(s) 189, 663
BpuEI CTTGAG 1 cut(s) 698
Bsa29I ATCGAT 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 54, 369
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 766, 899, 987
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 592
BseBI CCWGG 2 cut(s) 179, 272
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 54, 369
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 4 cut(s) 55, 766, 899, 987
BseMI GCAATG 1 cut(s) 592
BseMII CTCAG 2 cut(s) 654, 776
BseNI ACTGG 1 cut(s) 513
BsePI GCGCGC 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 251, 740
BseXI GCAGC 1 cut(s) 900
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 4 cut(s) 60, 194, 377, 575
BshVI ATCGAT 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 308
BsiWI CGTACG 1 cut(s) 713
BslI CCNNNNNNNGG 4 cut(s) 55, 766, 899, 987
BsmAI GTCTC 1 cut(s) 642
BsmBI CGTCTC 1 cut(s) 642
BsmI GAATGC 2 cut(s) 214, 460
BsnI GGCC 4 cut(s) 60, 194, 377, 575
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 5 cut(s) 42, 316, 358, 531, 702
BspACI CCGC 2 cut(s) 11, 121
BspANI GGCC 4 cut(s) 60, 194, 377, 575
BspCNI CTCAG 2 cut(s) 655, 777
BspDI ATCGAT 1 cut(s) 41
BspFNI CGCG 1 cut(s) 186
BspHI TCATGA 1 cut(s) 319
BspPI GGATC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 592
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 54, 369
BssHII GCGCGC 1 cut(s) 184
BssMI GATC 5 cut(s) 42, 316, 358, 531, 702
BssT1I CCWWGG 2 cut(s) 54, 369
Bst2UI CCWGG 2 cut(s) 179, 272
Bst4CI ACNGT 1 cut(s) 842
Bst6I CTCTTC 1 cut(s) 717
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 3 cut(s) 186, 308, 366
BstDEI CTNAG 6 cut(s) 189, 663, 698, 785, 879, 984
BstENI CCTNNNNNAGG 1 cut(s) 985
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 186
BstHHI GCGC 4 cut(s) 186, 188, 268, 867
BstKTI GATC 5 cut(s) 45, 319, 361, 534, 705
BstMAI GTCTC 1 cut(s) 642
BstMBI GATC 5 cut(s) 42, 316, 358, 531, 702
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 3 cut(s) 374, 554, 873
BstNI CCWGG 2 cut(s) 179, 272
BstSCI CCNGG 2 cut(s) 177, 270
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 900
BstV2I GAAGAC 2 cut(s) 489, 504
Bsu15I ATCGAT 1 cut(s) 41
BsuRI GGCC 4 cut(s) 60, 194, 377, 575
BsuTUI ATCGAT 1 cut(s) 41
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 847, 922
Cac8I GCNNGC 3 cut(s) 186, 308, 366
CciI TCATGA 1 cut(s) 319
CfoI GCGC 4 cut(s) 186, 188, 268, 867
Cfr13I GGNCC 1 cut(s) 58
ClaI ATCGAT 1 cut(s) 41
CseI GACGC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 270
Csp6I GTAC 4 cut(s) 136, 445, 714, 793
CviAII CATG 2 cut(s) 320, 594
CviQI GTAC 4 cut(s) 136, 445, 714, 793
DdeI CTNAG 6 cut(s) 189, 663, 698, 785, 879, 984
DpnI GATC 5 cut(s) 44, 318, 360, 533, 704
DpnII GATC 5 cut(s) 42, 316, 358, 531, 702
DraIII CACNNNGTG 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco130I CCWWGG 2 cut(s) 54, 369
Eco57I CTGAAG 3 cut(s) 110, 501, 741
Eco72I CACGTG 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 985
EcoRII CCWGG 2 cut(s) 177, 270
EcoT14I CCWWGG 2 cut(s) 54, 369
EcoT22I ATGCAT 1 cut(s) 462
ErhI CCWWGG 2 cut(s) 54, 369
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 2 cut(s) 323, 597
FaiI YATR 6 cut(s) 30, 206, 321, 439, 595, 834
FatI CATG 2 cut(s) 319, 593
FbaI TGATCA 2 cut(s) 358, 531
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 2 cut(s) 122, 889
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 122, 889
FspAI RTGCGCAY 1 cut(s) 267
FspBI CTAG 3 cut(s) 656, 761, 975
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 4 cut(s) 185, 187, 267, 866
GluI GCNGC 2 cut(s) 122, 889
GsuI CTGGAG 2 cut(s) 200, 590
HaeIII GGCC 4 cut(s) 60, 194, 377, 575
HgaI GACGC 1 cut(s) 82
HhaI GCGC 4 cut(s) 186, 188, 268, 867
Hin1II CATG 2 cut(s) 323, 597
Hin6I GCGC 4 cut(s) 184, 186, 266, 865
HinP1I GCGC 4 cut(s) 184, 186, 266, 865
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 216
HphI GGTGA 2 cut(s) 286, 939
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 4 cut(s) 106, 129, 246, 413
Hpy188III TCNNGA 5 cut(s) 151, 320, 392, 569, 975
Hpy8I GTNNAC 1 cut(s) 72
Hpy99I CGWCG 3 cut(s) 26, 76, 637
HpyAV CCTTC 2 cut(s) 676, 793
HpyCH4III ACNGT 1 cut(s) 842
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 6 cut(s) 212, 285, 460, 557, 599, 845
HpyF10VI GCNNNNNNNGC 3 cut(s) 374, 554, 873
HpyF3I CTNAG 6 cut(s) 189, 663, 698, 785, 879, 984
HpySE526I ACGT 1 cut(s) 559
Hsp92II CATG 2 cut(s) 323, 597
HspAI GCGC 4 cut(s) 184, 186, 266, 865
Ksp22I TGATCA 2 cut(s) 358, 531
Kzo9I GATC 5 cut(s) 42, 316, 358, 531, 702
LmnI GCTCC 2 cut(s) 107, 181
Lsp1109I GCAGC 1 cut(s) 900
LweI GCATC 3 cut(s) 89, 447, 854
MabI ACCWGGT 1 cut(s) 270
MaeI CTAG 3 cut(s) 656, 761, 975
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 2 cut(s) 274, 959
MalI GATC 5 cut(s) 44, 318, 360, 533, 704
MboI GATC 5 cut(s) 42, 316, 358, 531, 702
MfeI CAATTG 1 cut(s) 492
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 6 cut(s) 86, 155, 248, 428, 492, 734
MlyI GAGTC 2 cut(s) 271, 599
MmeI TCCRAC 1 cut(s) 378
Mph1103I ATGCAT 1 cut(s) 462
MroXI GAANNNNTTC 1 cut(s) 811
MslI CAYNNNNRTG 1 cut(s) 259
MspR9I CCNGG 2 cut(s) 179, 272
MunI CAATTG 1 cut(s) 492
Mva1269I GAATGC 2 cut(s) 214, 460
MvaI CCWGG 2 cut(s) 179, 272
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 374, 554, 873
NdeII GATC 5 cut(s) 42, 316, 358, 531, 702
NlaIII CATG 2 cut(s) 323, 597
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 274
NsbI TGCGCA 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 462
PagI TCATGA 1 cut(s) 319
PauI GCGCGC 1 cut(s) 184
PctI GAATGC 2 cut(s) 214, 460
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 8 cut(s) 4, 38, 130, 241, 382, 470, 512, 895
Pfl23II CGTACG 1 cut(s) 713
PflFI GACNNNGTC 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 55
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 2 cut(s) 123, 890
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 2 cut(s) 271, 598
PmaCI CACGTG 1 cut(s) 560
PmlI CACGTG 1 cut(s) 560
PpsI GAGTC 2 cut(s) 271, 598
Ppu21I YACGTR 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 177, 270
PspCI CACGTG 1 cut(s) 560
PspGI CCWGG 2 cut(s) 177, 270
PspLI CGTACG 1 cut(s) 713
PspPI GGNCC 1 cut(s) 58
PsyI GACNNNGTC 1 cut(s) 407
PteI GCGCGC 1 cut(s) 184
PvuI CGATCG 1 cut(s) 45
RsaI GTAC 4 cut(s) 137, 446, 715, 794
RsaNI GTAC 4 cut(s) 136, 445, 714, 793
RseI CAYNNNNRTG 1 cut(s) 259
SalI GTCGAC 1 cut(s) 70
SatI GCNGC 2 cut(s) 122, 889
Sau3AI GATC 5 cut(s) 42, 316, 358, 531, 702
Sau96I GGNCC 1 cut(s) 58
ScaI AGTACT 1 cut(s) 137
SchI GAGTC 2 cut(s) 271, 599
ScrFI CCNGG 2 cut(s) 179, 272
SduI GDGCHC 1 cut(s) 308
SetI ASST 9 cut(s) 220, 273, 550, 562, 669, 732, 878, 983, 992
SexAI ACCWGGT 1 cut(s) 270
SfaNI GCATC 3 cut(s) 89, 447, 854
SmiMI CAYNNNNRTG 1 cut(s) 259
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
SpeI ACTAGT 1 cut(s) 655
Sse9I AATT 6 cut(s) 86, 155, 248, 428, 492, 734
SsiI CCGC 2 cut(s) 11, 121
SspMI CTAG 3 cut(s) 656, 761, 975
StyD4I CCNGG 2 cut(s) 177, 270
StyI CCWWGG 2 cut(s) 54, 369
TaaI ACNGT 1 cut(s) 842
TaiI ACGT 1 cut(s) 562
TaqI TCGA 7 cut(s) 21, 41, 45, 71, 84, 97, 152
TaqII GACCGA 1 cut(s) 339
TasI AATT 6 cut(s) 86, 155, 248, 428, 492, 734
TatI WGTACW 2 cut(s) 135, 444
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 8 cut(s) 4, 38, 130, 241, 382, 470, 512, 895
TscAI CASTG 2 cut(s) 847, 922
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 1 cut(s) 888
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 5 cut(s) 17, 221, 441, 462, 558
TspRI CASTG 2 cut(s) 847, 922
Tth111I GACNNNGTC 1 cut(s) 407
Van91I CCANNNNNTGG 1 cut(s) 55
XagI CCTNNNNNAGG 1 cut(s) 985
XbaI TCTAGA 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 3 cut(s) 656, 761, 975
ZrmI AGTACT 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.