Rorug07G0311900
NAC Family

(NAC) domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000497
Physical Location & Seq
Reverse (-)
31250668 .. 31254685
4018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0311900.1

Sequence Viewer

Length: 2256 bp
ATGGAAGACGTAGCGAAGCTGAAGAACATAATTTATAGGGAAGAAAATCAGGTCAGCGAGAGTGCTGTTGAAGTGGCCAGTCTGACAACTGATGATGATTGCAAGACATTGTCCGGTGTTAATGACGAATCAGACCTCGAGGTTACAATCAGAACAGCGTGTCGAGGAAAACACTTCAGAAGAACTATGTCTTCCCTGTCAGATGAAAAGGTAGCTGCGACATATGAAATTGGGTTTGGGTCACTGCACCGATTTCGTTGTGGCAAAGTGAACCTCCCACTGTGCCAAATGCTGGTTGAAAATTTTGATGTTGGGAACTCATGCATCAAGATACACGGAAGGAACCTGACAATCACACATGAAGACTTTCGGCGCATTATGGGCGTCATGGATGGAGGGTGCGATGTGAATCTTGAAGGAAGCACCGAGGACCCTGATATACTGGGGCTGAAGAGCAAGCTTTCAAGGAAGGAGAAGGAGATAACCATACATGGTCTGAGGAGGTTGTTGATTGAATCTGAAAGTACCGACGACACTTTTAAGGTCAGCTTTGCTTTGTATGCCCTTGCAACTCTGTTAATCCCAGTTACGGGAATCGAAGTTGATCTAAGATACCTTATACCACTTAAGGATCCTAATGCCCTGGGTTCAAAAAACTGGGCAAAGTTTGCATTCGCCAAGCTTGTTGAAGGGGTGTCATCATTCCAGGCCACGCGGACTGGTTTGGCAGGAGGTTGTATTCTGTTCCTTCAATTGTTCTACCTGGATGTTGTTGGCAATGGTATCTGTATATATCCAAAGATTGGGAAACCAGTGATGACGTGGAGCAGAGATGACTTGAAGAGAGTTTATGAAAAAGTGGAGAGCGAGGGCGGATTTAAAAGCGAGAATGTGCGTGTAACGAAGAAGTATTTCAGGGGAAATGTACTTTCGGGGACTGGATCAGTTGATCCCAGCACAGGGATTAAGACAACTGTAGTAGACGATTTATCAGAAGTGAGGTCTGATGTTGGTCTTGTGAAAGGGGATATAGAGAGTTTGAAGTTAACGGTTGGTCGGTTGGAGCCAGACATGGGTCGATTGAGGAGAGCAATTCTGACACTAGAATCCACGGTTGGGGAACTTAAGGCCAACGGTCTCGGCGGCATTGTTGCTGAAGCAATCAAGCAAGTGTTTGAAGACAAGTCCTACACTTACGGTGGTAATTTCGGGAACGATGGTCTTTTTATCAATGAAGAGCACCAAGGCACAGAAGCTGAGCAACATGACGATGAGATGCCTCATAACAAATCTATCATTGTCATCTCCACTGACAAGGATGCATATGGATGCGACAGCAGTGACATGGAAGAAGATGAGGCAGGGAGTTTCCCGGCGGTGTCTGAGATATCGCACAGCGACTCATACCTACTGACCTATCTATTCAGCAAGAAGGTTACCGCTGACGACAGTGTTGGGAGCAATGAAGTTGCTAGGCACGAGGAATGGTCTGTTTCTCGATGGGACATGAGGAGCTTGTCCCCAATGCAATGTCTGACCTATGAGATTGTTGACATATCGGCTGCTTACTTGTTTGATCGGGGATCGGACGACTGGTTCATGCCAACTTCATTCTCAGAACGTGCTAAGGAGTATTTAGCGGGTGTGCAGGTGGAAGGTCTCGTGCCATCTACCGTTAAACTGTGCCGACTGGGAAGATTCATTGGTAGATTTATGTCGTGCAAAAAGATTTTCTTTCCTATCCATGACGGCGTACTTAAGCATTGGTTCTTGGCAGTTCTTAAAATACCCAGTTGGCAATGCGAAATATGGGACAGCAACCCCGAGAAGTCAGCAAACAAACGCCGCGAGGAATATGTTGCTGCATCTATATCGATGATTGAAAAAGTTTTTGCTGCTGCAATGCAATTGTCACCTGGCATTCATGCCAAGATTGGTTCATTGTCCATCAAGTATCCACAGAAAAGCCCTAGGCATCCCAACAGCCAAGACTCGGGAATATTTCTGATTAGAAGCATGCAGTTTTACCGTCAGCGCTGGTACCAAGGGTTTAACTCGGGGGACCAGAGGGTTCGTATGGCACTTGAGACAGTGAATCACCCAAAGAATGAAGTTCTGGAACAGGTCTTTGAAGCCGCTGCAGAGGAAGTCCCATATGATGTAGCATCTGCATGGTTGGCACAAGAATATTTGAACAAAAATGGAAGTCTCCCAACTGCAGGCATGAAGTTCAACCCACGCCGGGCTAGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

751

Amino Acids

83.95

Weight (kDa)

5.7

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 571 - 679 3.3e-11 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000497)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14680 AT4G09060 AT4G09060
fragaria_vesca FvH4_5g36300 FvH4_5g36300
malus_domestica MD08G1189700.v1.1 MD08G1190000.v1.1 MD08G1190200.v1.1 MD15G1377500.v1.1
prunus_persica Prupe.1G524200_v2.0.a1
pyrus_communis pycom08g16320 pycom15g33820
rosa_chinensis RchiOBHm_Chr3g0481851 RchiOBHm_Chr3g0481861 RchiOBHm_Chr5g0040961 RchiOBHm_Chr5g0044491 RchiOBHm_Chr5g0044811 RchiOBHm_Chr5g0045611 RchiOBHm_Chr5g0045621 RchiOBHm_Chr5g0049341 RchiOBHm_Chr5g0049351 RchiOBHm_Chr7g0238301 RchiOBHm_Chr7g0238321
rosa_laevigata RLG00000000918 RLG00000000921 RLG00000034299 RLG00000034300
rosa_multiflora Rmu_sc0001047.1_g000051 Rmu_sc0001371.1_g000020 Rmu_sc0001847.1_g000002 Rmu_sc0002988.1_g000004 Rmu_sc0003789.1_g000001 Rmu_sc0004567.1_g000016 Rmu_sc0004800.1_g000003 Rmu_sc0005102.1_g000001 Rmu_sc0006651.1_g000010 Rmu_sc0006651.1_g000011 Rmu_ssc0000398.1_g000017
rosa_roxburghii Rroxscaffold_153G00436700 Rroxscaffold_153G00436710 Rroxscaffold_1G00034810 Rroxscaffold_1G00036220 Rroxscaffold_1G00036230 Rroxscaffold_1G00074790 Rroxscaffold_2G00104790 Rroxscaffold_2G00104800 Rroxscaffold_3G00223120 Rroxscaffold_5G00337240 Rroxscaffold_6G00399830 Rroxscaffold_6G00400170
rosa_rugosa Rorug02G0148000 Rorug04G0003000 Rorug05G0218300 Rorug05G0218400 Rorug05G0219900 Rorug07G0311100 Rorug07G0311900
rosa_samantha Rh2BG555200 Rh3DG293700 Rh4BG116500 Rh5BG279700 Rh5BG308000 Rh5BG308100 Rh5BG313100 Rh5BG313200 Rh5BG335300 Rh5BG335400 Rh5CG335000 Rh5CG335100 Rh5DG287700 Rh5DG318100 Rh5DG318200 Rh5DG323600 Rh5DG323700 Rh5DG347600 Rh5DG347700 Rh6DG317800 Rh7BG437500 Rh7CG484600 Rh7DG453000
rosa_wichuraiana Rw0G004340 Rw5G025810 Rw5G027210 Rw5G027900 Rw5G028300 Rw5G030640 Rw7G038670 Rw7G038780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1639
AbsI CCTCGAGG 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 1639
Acc65I GGTACC 1 cut(s) 2040
AccB1I GGYRCC 1 cut(s) 2040
AccB7I CCANNNNNTGG 2 cut(s) 292, 803
AccI GTMKAC 1 cut(s) 981
AccII CGCG 2 cut(s) 715, 1848
AciI CCGC 8 cut(s) 715, 873, 1143, 1376, 1440, 1640, 1846, 2136
AclWI GGATC 5 cut(s) 626, 639, 944, 949, 1591
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 4 cut(s) 41, 160, 470, 1176
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 4 cut(s) 526, 927, 1755, 2042
AfeI AGCGCT 1 cut(s) 2036
AflII CTTAAG 3 cut(s) 626, 1124, 1757
AjiI CACGTC 1 cut(s) 822
AjnI CCWGG 4 cut(s) 642, 705, 762, 1915
AjuI GAANNNNNNNTTGG 4 cut(s) 219, 251, 1098, 1130
AloI GAACNNNNNNTCC 2 cut(s) 1580, 1612
AluBI AGCT 7 cut(s) 19, 215, 460, 549, 682, 1256, 1515
AluI AGCT 7 cut(s) 19, 215, 460, 549, 682, 1256, 1515
Alw21I GWGCWC 1 cut(s) 1242
Alw26I GTCTC 4 cut(s) 1142, 1664, 2081, 2213
AlwI GGATC 5 cut(s) 626, 639, 944, 949, 1591
AlwNI CAGNNNCTG 1 cut(s) 1256
Ama87I CYCGRG 4 cut(s) 137, 1823, 1993, 2056
Aor51HI AGCGCT 1 cut(s) 2036
AoxI GGCC 3 cut(s) 75, 708, 1128
ApeKI GCWGC 6 cut(s) 215, 1562, 1862, 1895, 1898, 2138
ApoI RAATTY 1 cut(s) 301
Asp700I GAANNNNTTC 2 cut(s) 366, 911
Asp718I GGTACC 1 cut(s) 2040
AspA2I CCTAGG 1 cut(s) 1970
AspLEI GCGC 3 cut(s) 375, 2037, 2253
AspS9I GGNCC 2 cut(s) 430, 2062
AsuC2I CCSGG 2 cut(s) 1373, 2243
AsuHPI GGTGA 2 cut(s) 1905, 2090
AvaI CYCGRG 4 cut(s) 137, 1823, 1993, 2056
AvaII GGWCC 2 cut(s) 430, 2062
AvrII CCTAGG 1 cut(s) 1970
BalI TGGCCA 1 cut(s) 77
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 2040
BauI CACGAG 2 cut(s) 1478, 1661
BbsI GAAGAC 4 cut(s) 12, 183, 369, 1185
Bbv12I GWGCWC 1 cut(s) 1242
BbvI GCAGC 6 cut(s) 202, 1549, 1849, 1882, 1885, 2125
BccI CCATC 5 cut(s) 386, 1211, 1494, 1675, 1955
BceAI ACGGC 1 cut(s) 1765
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 1854, 1888
BciT130I CCWGG 4 cut(s) 644, 707, 764, 1917
BciVI GTATCC 1 cut(s) 1965
BcnI CCSGG 2 cut(s) 1373, 2243
BcoDI GTCTC 4 cut(s) 1142, 1664, 2081, 2213
BfaI CTAG 4 cut(s) 1103, 1473, 1971, 2247
BfmI CTRYAG 3 cut(s) 975, 2139, 2217
BfoI RGCGCY 2 cut(s) 2038, 2254
BfrI CTTAAG 3 cut(s) 626, 1124, 1757
BfuAI ACCTGC 1 cut(s) 1639
BfuI GTATCC 1 cut(s) 1965
BisI GCNGC 9 cut(s) 216, 1144, 1563, 1846, 1863, 1896, 1899, 2136, 2139
BlnI CCTAGG 1 cut(s) 1970
BlpI GCTNAGC 1 cut(s) 1257
BlsI GCNGC 9 cut(s) 217, 1145, 1564, 1847, 1864, 1897, 1900, 2137, 2140
Bme1390I CCNGG 6 cut(s) 644, 707, 764, 1373, 1917, 2243
Bme18I GGWCC 2 cut(s) 430, 2062
BmeT110I CYCGRG 4 cut(s) 137, 1823, 1993, 2056
BmgBI CACGTC 1 cut(s) 822
BmgT120I GGNCC 2 cut(s) 430, 2062
BmiI GGNNCC 6 cut(s) 344, 432, 633, 1065, 2042, 2063
BmrFI CCNGG 6 cut(s) 644, 707, 764, 1373, 1917, 2243
BmrI ACTGGG 5 cut(s) 452, 578, 667, 1700, 1785
BmsI GCATC 7 cut(s) 333, 1266, 1309, 1319, 1874, 1984, 2174
BmuI ACTGGG 5 cut(s) 452, 578, 667, 1700, 1785
BoxI GACNNNNGTC 1 cut(s) 1074
BpiI GAAGAC 4 cut(s) 12, 183, 369, 1185
Bpu10I CCTNAGC 1 cut(s) 1626
Bpu1102I GCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 1 cut(s) 2105
BpuMI CCSGG 2 cut(s) 1373, 2243
Bsa29I ATCGAT 1 cut(s) 1874
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 2 cut(s) 1142, 1664
BsaJI CCNNGG 7 cut(s) 426, 642, 643, 1110, 1243, 1970, 2044
BsaWI WCCGGW 1 cut(s) 113
Bse3DI GCAATG 5 cut(s) 784, 1468, 1535, 1805, 1908
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 4 cut(s) 644, 707, 764, 1917
BseCI ATCGAT 1 cut(s) 1874
BseDI CCNNGG 7 cut(s) 426, 642, 643, 1110, 1243, 1970, 2044
BseGI GGATG 5 cut(s) 397, 772, 1324, 1334, 1975
BseJI GATNNNNATC 1 cut(s) 408
BseMI GCAATG 5 cut(s) 784, 1468, 1535, 1805, 1908
BseMII CTCAG 4 cut(s) 488, 1248, 1374, 1629
BseRI GAGGAG 3 cut(s) 514, 1099, 1525
BseXI GCAGC 6 cut(s) 202, 1549, 1849, 1882, 1885, 2125
BseYI CCCAGC 1 cut(s) 953
BsgI GTGCAG 2 cut(s) 230, 1667
Bsh1236I CGCG 2 cut(s) 715, 1848
BshFI GGCC 3 cut(s) 77, 710, 1130
BshNI GGYRCC 1 cut(s) 2040
BshVI ATCGAT 1 cut(s) 1874
BsiHKAI GWGCWC 1 cut(s) 1242
BsiHKCI CYCGRG 4 cut(s) 137, 1823, 1993, 2056
BsiSI CCGG 3 cut(s) 114, 1373, 2242
BslFI GGGAC 6 cut(s) 949, 1504, 1517, 1826, 2075, 2135
BsmAI GTCTC 4 cut(s) 1142, 1664, 2081, 2213
BsmFI GGGAC 6 cut(s) 949, 1504, 1517, 1826, 2075, 2135
BsmI GAATGC 2 cut(s) 671, 1920
BsnI GGCC 3 cut(s) 77, 710, 1130
Bso31I GGTCTC 2 cut(s) 1142, 1664
BsoBI CYCGRG 4 cut(s) 137, 1823, 1993, 2056
Bsp1286I GDGCHC 1 cut(s) 1242
Bsp143I GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
Bsp1720I GCTNAGC 1 cut(s) 1257
BspACI CCGC 8 cut(s) 715, 873, 1143, 1376, 1440, 1640, 1846, 2136
BspANI GGCC 3 cut(s) 77, 710, 1130
BspCNI CTCAG 4 cut(s) 489, 1249, 1375, 1628
BspDI ATCGAT 1 cut(s) 1874
BspFNI CGCG 2 cut(s) 715, 1848
BspLI GGNNCC 6 cut(s) 344, 432, 633, 1065, 2042, 2063
BspMAI CTGCAG 2 cut(s) 2143, 2221
BspMI ACCTGC 1 cut(s) 1639
BspPI GGATC 5 cut(s) 626, 639, 944, 949, 1591
BspQI GCTCTTC 2 cut(s) 446, 1230
BspT107I GGYRCC 1 cut(s) 2040
BspTI CTTAAG 3 cut(s) 626, 1124, 1757
BspTNI GGTCTC 2 cut(s) 1142, 1664
BsrDI GCAATG 5 cut(s) 784, 1468, 1535, 1805, 1908
BssECI CCNNGG 7 cut(s) 426, 642, 643, 1110, 1243, 1970, 2044
BssMI GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 2 cut(s) 1478, 1661
BssT1I CCWWGG 3 cut(s) 1243, 1970, 2044
Bst2BI CACGAG 2 cut(s) 1478, 1661
Bst2UI CCWGG 4 cut(s) 644, 707, 764, 1917
Bst6I CTCTTC 3 cut(s) 446, 836, 1230
BstACI GRCGYC 1 cut(s) 384
BstAFI CTTAAG 3 cut(s) 626, 1124, 1757
BstAPI GCANNNNNTGC 1 cut(s) 668
BstC8I GCNNGC 3 cut(s) 458, 2018, 2221
BstDEI CTNAG 6 cut(s) 497, 608, 1257, 1383, 1615, 1626
BstDSI CCRYGG 1 cut(s) 1110
BstEII GGTNACC 1 cut(s) 1435
BstF5I GGATG 5 cut(s) 397, 772, 1324, 1334, 1975
BstFNI CGCG 2 cut(s) 715, 1848
BstH2I RGCGCY 2 cut(s) 2038, 2254
BstHHI GCGC 3 cut(s) 375, 2037, 2253
BstKTI GATC 6 cut(s) 607, 634, 944, 952, 1579, 1586
BstMAI GTCTC 4 cut(s) 1142, 1664, 2081, 2213
BstMBI GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
BstMWI GCNNNNNNNGC 4 cut(s) 381, 560, 668, 2177
BstNI CCWGG 4 cut(s) 644, 707, 764, 1917
BstNSI RCATGY 1 cut(s) 2020
BstPAI GACNNNNGTC 1 cut(s) 1074
BstPI GGTNACC 1 cut(s) 1435
BstSCI CCNGG 6 cut(s) 642, 705, 762, 1371, 1915, 2241
BstSFI CTRYAG 3 cut(s) 975, 2139, 2217
BstUI CGCG 2 cut(s) 715, 1848
BstV1I GCAGC 6 cut(s) 202, 1549, 1849, 1882, 1885, 2125
BstV2I GAAGAC 4 cut(s) 12, 183, 369, 1185
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
Bsu15I ATCGAT 1 cut(s) 1874
BsuI GTATCC 1 cut(s) 1965
BsuRI GGCC 3 cut(s) 77, 710, 1130
BsuTUI ATCGAT 1 cut(s) 1874
BtgI CCRYGG 1 cut(s) 1110
BtgZI GCGATG 1 cut(s) 417
BtrI CACGTC 1 cut(s) 822
BtsCI GGATG 5 cut(s) 397, 772, 1324, 1334, 1975
BtsI GCAGTG 2 cut(s) 242, 1345
BtsIMutI CAGTG 7 cut(s) 242, 278, 819, 1308, 1345, 1456, 2097
BveI ACCTGC 1 cut(s) 1639
Cac8I GCNNGC 3 cut(s) 458, 2018, 2221
CaiI CAGNNNCTG 1 cut(s) 1256
CfoI GCGC 3 cut(s) 375, 2037, 2253
Cfr13I GGNCC 2 cut(s) 430, 2062
ClaI ATCGAT 1 cut(s) 1874
CseI GACGC 1 cut(s) 373
Csp6I GTAC 4 cut(s) 525, 926, 1754, 2041
CviQI GTAC 4 cut(s) 525, 926, 1754, 2041
DdeI CTNAG 6 cut(s) 497, 608, 1257, 1383, 1615, 1626
DpnI GATC 6 cut(s) 606, 633, 943, 951, 1578, 1585
DpnII GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
DraI TTTAAA 1 cut(s) 880
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 3 cut(s) 446, 836, 1230
EarI CTCTTC 3 cut(s) 446, 836, 1230
EciI GGCGGA 1 cut(s) 888
Eco130I CCWWGG 3 cut(s) 1243, 1970, 2044
Eco31I GGTCTC 2 cut(s) 1142, 1664
Eco32I GATATC 1 cut(s) 1389
Eco47I GGWCC 2 cut(s) 430, 2062
Eco47III AGCGCT 1 cut(s) 2036
Eco57I CTGAAG 4 cut(s) 41, 160, 470, 1176
Eco88I CYCGRG 4 cut(s) 137, 1823, 1993, 2056
Eco91I GGTNACC 1 cut(s) 1435
EcoO109I RGGNCCY 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 1435
EcoRII CCWGG 4 cut(s) 642, 705, 762, 1915
EcoRV GATATC 1 cut(s) 1389
EcoT14I CCWWGG 3 cut(s) 1243, 1970, 2044
EcoT22I ATGCAT 2 cut(s) 326, 1324
ErhI CCWWGG 3 cut(s) 1243, 1970, 2044
FalI AAGNNNNNCTT 4 cut(s) 533, 565, 1718, 1750
FaqI GGGAC 6 cut(s) 949, 1504, 1517, 1826, 2075, 2135
FauI CCCGC 1 cut(s) 1633
FauNDI CATATG 3 cut(s) 223, 1324, 2155
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 9 cut(s) 216, 1144, 1563, 1846, 1863, 1896, 1899, 2136, 2139
FokI GGATG 5 cut(s) 404, 779, 1331, 1341, 1962
Fsp4HI GCNGC 9 cut(s) 216, 1144, 1563, 1846, 1863, 1896, 1899, 2136, 2139
FspBI CTAG 4 cut(s) 1103, 1473, 1971, 2247
GlaI GCGC 3 cut(s) 374, 2036, 2252
GluI GCNGC 9 cut(s) 216, 1144, 1563, 1846, 1863, 1896, 1899, 2136, 2139
GsaI CCCAGC 1 cut(s) 957
HaeII RGCGCY 2 cut(s) 2038, 2254
HaeIII GGCC 3 cut(s) 77, 710, 1130
HapII CCGG 3 cut(s) 114, 1373, 2242
HgaI GACGC 1 cut(s) 373
HhaI GCGC 3 cut(s) 375, 2037, 2253
Hin1I GRCGYC 1 cut(s) 384
Hin6I GCGC 3 cut(s) 373, 2035, 2251
HinP1I GCGC 3 cut(s) 373, 2035, 2251
HincII GTYRAC 2 cut(s) 1047, 1552
HindII GTYRAC 2 cut(s) 1047, 1552
HindIII AAGCTT 2 cut(s) 458, 680
HinfI GANTC 9 cut(s) 128, 409, 515, 594, 1106, 1400, 1698, 1991, 2095
HpaI GTTAAC 1 cut(s) 1047
HpaII CCGG 3 cut(s) 114, 1373, 2242
HphI GGTGA 2 cut(s) 1905, 2090
Hpy166II GTNNAC 4 cut(s) 271, 982, 1047, 1552
Hpy188III TCNNGA 6 cut(s) 328, 413, 1210, 1497, 1995, 2117
Hpy8I GTNNAC 4 cut(s) 271, 982, 1047, 1552
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 8 cut(s) 333, 410, 463, 469, 683, 758, 1426, 1649
HpyCH4IV ACGT 3 cut(s) 9, 821, 1621
HpyF10VI GCNNNNNNNGC 4 cut(s) 381, 560, 668, 2177
HpyF3I CTNAG 6 cut(s) 497, 608, 1257, 1383, 1615, 1626
HpySE526I ACGT 3 cut(s) 9, 821, 1621
Hsp92I GRCGYC 1 cut(s) 384
HspAI GCGC 3 cut(s) 373, 2035, 2251
KpnI GGTACC 1 cut(s) 2044
KspAI GTTAAC 1 cut(s) 1047
Kzo9I GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
LguI GCTCTTC 2 cut(s) 446, 1230
LmnI GCTCC 4 cut(s) 825, 1063, 1458, 1512
Lsp1109I GCAGC 6 cut(s) 202, 1549, 1849, 1882, 1885, 2125
LweI GCATC 7 cut(s) 333, 1266, 1309, 1319, 1874, 1984, 2174
MaeI CTAG 4 cut(s) 1103, 1473, 1971, 2247
MaeII ACGT 3 cut(s) 9, 821, 1621
MaeIII GTNAC 7 cut(s) 142, 240, 586, 898, 1340, 1435, 1911
MalI GATC 6 cut(s) 606, 633, 943, 951, 1578, 1585
MboI GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
MfeI CAATTG 2 cut(s) 752, 1907
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 1242
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 7 cut(s) 30, 228, 301, 752, 1092, 1204, 1907
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 2 cut(s) 1394, 1985
MmeI TCCRAC 1 cut(s) 1041
Mox20I TGGCCA 1 cut(s) 77
Mph1103I ATGCAT 2 cut(s) 326, 1324
MroXI GAANNNNTTC 2 cut(s) 366, 911
MscI TGGCCA 1 cut(s) 77
MslI CAYNNNNRTG 3 cut(s) 1269, 1327, 2170
Msp20I TGGCCA 1 cut(s) 77
MspA1I CMGCKG 2 cut(s) 1442, 2138
MspCI CTTAAG 3 cut(s) 626, 1124, 1757
MspI CCGG 3 cut(s) 114, 1373, 2242
MspR9I CCNGG 6 cut(s) 644, 707, 764, 1373, 1917, 2243
MunI CAATTG 2 cut(s) 752, 1907
Mva1269I GAATGC 2 cut(s) 671, 1920
MvaI CCWGG 4 cut(s) 644, 707, 764, 1917
MvnI CGCG 2 cut(s) 715, 1848
MwoI GCNNNNNNNGC 4 cut(s) 381, 560, 668, 2177
NciI CCSGG 2 cut(s) 1373, 2243
NdeI CATATG 3 cut(s) 223, 1324, 2155
NdeII GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
NlaIV GGNNCC 6 cut(s) 344, 432, 633, 1065, 2042, 2063
NmeAIII GCCGAG 1 cut(s) 1119
NmuCI GTSAC 3 cut(s) 240, 1340, 1911
NsiI ATGCAT 2 cut(s) 326, 1324
NspI RCATGY 1 cut(s) 2020
PaeI GCATGC 1 cut(s) 2020
PaeR7I CTCGAG 1 cut(s) 137
PaqCI CACCTGC 1 cut(s) 1639
PasI CCCWGGG 1 cut(s) 643
PciSI GCTCTTC 2 cut(s) 446, 1230
PctI GAATGC 2 cut(s) 671, 1920
PdmI GAANNNNTTC 2 cut(s) 366, 911
PfeI GAWTC 7 cut(s) 128, 409, 515, 594, 1106, 1698, 2095
PflFI GACNNNGTC 1 cut(s) 109
PflMI CCANNNNNTGG 2 cut(s) 292, 803
PkrI GCNGC 9 cut(s) 217, 1145, 1564, 1847, 1864, 1897, 1900, 2137, 2140
PleI GAGTC 2 cut(s) 1394, 1985
PpsI GAGTC 2 cut(s) 1394, 1985
PpuMI RGGWCCY 1 cut(s) 430
PshAI GACNNNNGTC 1 cut(s) 1074
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 4 cut(s) 642, 705, 762, 1915
PspEI GGTNACC 1 cut(s) 1435
PspFI CCCAGC 1 cut(s) 953
PspGI CCWGG 4 cut(s) 642, 705, 762, 1915
PspN4I GGNNCC 6 cut(s) 344, 432, 633, 1065, 2042, 2063
PspPI GGNCC 2 cut(s) 430, 2062
PspPPI RGGWCCY 1 cut(s) 430
PspXI VCTCGAGB 1 cut(s) 137
PstI CTGCAG 2 cut(s) 2143, 2221
PstNI CAGNNNCTG 1 cut(s) 1256
PsuI RGATCY 1 cut(s) 631
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 4 cut(s) 526, 927, 1755, 2042
RsaNI GTAC 4 cut(s) 525, 926, 1754, 2041
RseI CAYNNNNRTG 3 cut(s) 1269, 1327, 2170
SapI GCTCTTC 2 cut(s) 446, 1230
SatI GCNGC 9 cut(s) 216, 1144, 1563, 1846, 1863, 1896, 1899, 2136, 2139
Sau3AI GATC 6 cut(s) 604, 631, 941, 949, 1576, 1583
Sau96I GGNCC 2 cut(s) 430, 2062
SchI GAGTC 2 cut(s) 1394, 1985
ScrFI CCNGG 6 cut(s) 644, 707, 764, 1373, 1917, 2243
SduI GDGCHC 1 cut(s) 1242
SfaNI GCATC 7 cut(s) 333, 1266, 1309, 1319, 1874, 1984, 2174
SfcI CTRYAG 3 cut(s) 975, 2139, 2217
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 2 cut(s) 430, 2062
SlaI CTCGAG 1 cut(s) 137
SmiMI CAYNNNNRTG 3 cut(s) 1269, 1327, 2170
SmlI CTYRAG 5 cut(s) 137, 626, 1124, 1757, 2084
SmoI CTYRAG 5 cut(s) 137, 626, 1124, 1757, 2084
SphI GCATGC 1 cut(s) 2020
Sse9I AATT 7 cut(s) 30, 228, 301, 752, 1092, 1204, 1907
SsiI CCGC 8 cut(s) 715, 873, 1143, 1376, 1440, 1640, 1846, 2136
SspI AATATT 2 cut(s) 2001, 2189
SspMI CTAG 4 cut(s) 1103, 1473, 1971, 2247
StyD4I CCNGG 6 cut(s) 642, 705, 762, 1371, 1915, 2241
StyI CCWWGG 3 cut(s) 1243, 1970, 2044
TaiI ACGT 3 cut(s) 12, 824, 1624
TaqI TCGA 6 cut(s) 138, 163, 597, 1078, 1498, 1874
TasI AATT 7 cut(s) 30, 228, 301, 752, 1092, 1204, 1907
TatI WGTACW 1 cut(s) 925
TauI GCSGC 3 cut(s) 1146, 1848, 2138
TfiI GAWTC 7 cut(s) 128, 409, 515, 594, 1106, 1698, 2095
TscAI CASTG 7 cut(s) 249, 285, 819, 1315, 1345, 1456, 2097
TseFI GTSAC 3 cut(s) 240, 1340, 1911
TseI GCWGC 6 cut(s) 215, 1562, 1862, 1895, 1898, 2138
Tsp45I GTSAC 3 cut(s) 240, 1340, 1911
TspGWI ACGGA 1 cut(s) 351
TspRI CASTG 7 cut(s) 249, 285, 819, 1315, 1345, 1456, 2097
Tth111I GACNNNGTC 1 cut(s) 109
Van91I CCANNNNNTGG 2 cut(s) 292, 803
Vha464I CTTAAG 3 cut(s) 626, 1124, 1757
VpaK11BI GGWCC 2 cut(s) 430, 2062
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 2020
XcmI CCANNNNNNNNNTGG 1 cut(s) 819
XhoI CTCGAG 1 cut(s) 137
XmaJI CCTAGG 1 cut(s) 1970
XmiI GTMKAC 1 cut(s) 981
XmnI GAANNNNTTC 2 cut(s) 366, 911
XspI CTAG 4 cut(s) 1103, 1473, 1971, 2247
Zsp2I ATGCAT 2 cut(s) 326, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.